萜类化合物的生产方法

相关申请的交叉引用

本申请要求于2018年8月31日提交的新加坡申请No.10201807514P的优先权，其内容通过引用整体并入本文以用于所有目的。

技术领域

本发明属于生物技术领域。具体地，本发明涉及用于发现真菌萜烯合酶的方法以及真菌萜烯合酶在生产萜类化合物中的用途。

背景技术

萜类化合物构成天然产物中结构最多样化的类别之一，其具有广泛的应用：作为药物(例如紫杉醇和青蒿素)，作为食品色素(例如类胡萝卜素)、调味剂和芳香剂(例如诺卡酮和香紫苏醇)和生物燃料(例如法呢烯)。萜类多样性主要归因于萜烯合酶(TPS)，其将无环异戊二烯基二磷酸前体(acyclic prenyl diphosphate precursor)转化为多种环状和无环萜烯骨架。具体地，萜烯骨架多样性源于TPS的两个主要特征：大量具有不同功能的TPS和许多TPS能够从单一底物催化多种萜烯产物的能力。除了它们的主要产物之外，几乎一半的所表征的单萜烯和倍半萜烯合酶都产生显著量的额外产物。

在结构上，来自植物、真菌和细菌的所有倍半萜烯合酶(STS)具有保守的金属结合基序(与植物STS是DDXXD不同，真菌STS是DE(N)XXD)和NSE三联体。然而，在基序之外，在植物和微生物TPS之间存在有限的序列相似性。过去数十年来已经发现并研究了许多植物TPS，然而，真菌萜烯合酶的研究滞后。目前，仅克隆了少数真菌TPS并对其功能进行了表征(少于50个，表1)。

真菌具有巨大的多样性(约500万种)且其数量至少是植物的10倍。每个真菌在担子菌门中平均具有10至20个假定的TPS同源物，表明真菌萜类和TPS基因代表着丰富但尚未开发的自然资源。除了发现新的TPS基因和萜类化合物外，与具有相同功能或产物的现有TPS相比，鉴定其他潜在更有效的TPS也很有价值。这是由于萜类化合物的各种应用以及萜类化合物中的巨大商业利益。在一些情况下，萜类化合物的生产受到萜烯合酶活性不足的限制。鉴定具有新产品、优异的活性和选择性的TPS酶将极大地有益于工业生物技术界的萜类化合物生产。

发明内容

在一方面，提供了包含一种或多种载体的细菌菌株，该载体编码

a)产生一种或多种萜烯前体的一种或多种酶；和

b)真菌萜烯合酶(FTPS)。

在另一方面，提供了一种基因修饰的1-脱氧木酮糖-5-磷酸合酶(DXS)酶，其中该基因修饰是在一个或多个氨基酸位置处的突变。

在另一方面，提供了一种基因修饰的真菌萜烯合酶(FTPS)，其中基因修饰是在一个或多个氨基酸位置处的突变。

在另一方面，提供了生产萜类化合物的方法，包括

a)在表达培养基中培养本文所述的细菌菌株，以及b)从所述表达培养基中分离萜类化合物。

在另一方面，提供了生产萜类化合物的方法，包括a)在表达培养基中培养包含编码本文所述的基因修饰的FTPS的载体的细菌菌株，以及b)从所述表达培养基中分离萜类化合物。

在另一方面，提供了由多核苷酸编码的真菌萜烯合酶(FTPS)，该多核苷酸包含选自由SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38和SEQ ID NO:39组成的组的核酸序列。

在另一方面，提供了分离的核酸分子，其包含选自由SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38和SEQ ID NO:39组成的组的核酸序列。

定义

如本文所用，术语“萜烯(terpene)”是指由植物、细菌、真菌和昆虫产生的一类有机化合物。萜烯的构建单元具有五碳异戊二烯单元。

如本文所用，术语“萜类化合物(terpenoid)”是指一大类衍生自萜烯且种类繁多的有机化合物并且包括萜烯。萜烯的构建单元具有五碳异戊二烯单元并且含有另外的官能团，典型地为含氧官能团。萜烯是萜类化合物的子集。

如本文所用，术语“萜烯前体(terpene precursor)”是指被通过萜烯合酶转化为萜烯或萜类化合物的底物。该底物可以转化为萜烯或萜类化合物。

如本文所用，术语“萜烯合酶”(terpene synthase，TPS)是指将萜烯前体转化为萜烯和/或萜类化合物的酶。术语“真菌萜烯合酶”(FTPS)是指分离自真菌的萜烯合酶。

如本文所用，在真菌萜烯合酶(FTPS)的上下文中，术语“UP”是指FTPS的结构域。UP结构域位于DW结构域的上游。

如本文所用，在FTPS的上下文中，术语“DW”是指FTPS的结构域。DW结构域位于UP结构域的下游。

附图说明

当结合非限制性实例和附图考虑时，参考详细描述将更好地理解本发明，其中：

图1显示了由柱状田头菇(A.aegerita)产生的挥发性萜类化合物。通过固相微萃取(SPME)对来自柱状田头菇培养物的挥发性代谢物进行采样，并通过GC-MS进行分析。鉴定的萜烯类化合物的结构和质谱分别示于图4和5中。

图2显示了用于提高DXS酶的溶解度和活性的实验结果。在所有测定中，S0是来自大肠杆菌的野生型DXS。(A)显示了诱导后5小时的溶解度和可溶性DXS。(B)显示了DXS及其突变体的体外酶活性测定。(C)显示了当使用不同的DXS突变体时的特异性番茄红素的产量。(d)显示了DXS突变体的注释。

图3显示了用于筛选萜烯合酶的工程化大肠杆菌菌株的示意图。通路中的代谢产物为：GAP，甘油醛-3-磷酸；DXP，1-脱氧-D-木酮糖-5-磷酸；MEP，甲基赤藓醇磷酸酯；CDP-ME，4-二磷酸胞苷-2-C-甲基-D-赤藓醇；CDPMEP，4-二磷酸胞苷-2-C-甲基-D-赤藓醇-2-磷酸酯；MEC，2-C-甲基-D-赤藓醇-2,4-二磷酸酯；HMBPP，1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸酯；IPP，焦磷酸异戊烯酯；DMAPP，二甲基烯丙基焦磷酸酯；GPP，焦磷酸香叶酯；FPP，焦磷酸法尼酯和GGPP，焦磷酸香叶基香叶酯。酶为：dxs，DXP合酶；dxr，DXP还原酶；ispD，CDPME合酶；ispE，CDPME激酶；ispF，CDPMEP合酶；ispG，MBPP合酶；ispH，HMBPP还原酶；idi，IPP异构酶；STS，倍半萜烯合酶和MTS，单萜烯合酶。

图4显示了本研究中鉴定的萜烯类化合物。

图5显示了本研究中分析的萜烯类化合物的质谱。

图6显示了柱状田头菇中预测的生物合成基因簇。四个假定的TPS(AAE3J39008、AAE3J36743、AAE3J34444和AAE3J35024)编译成一个簇。STS基因中的4个(AAE3_10454、AAE3_12839、AAE3_04120和AAE3_13291)是簇的一部分，其中包括两个到五个P450单加氧酶。

图7显示了由表达来自柱状田头菇的TPS基因的大肠杆菌生产萜烯。代谢物用DB5柱通过GC-MS进行分析。用VF-WAXms柱通过GC-MS分析的代谢物谱图如图8所示。主要化合物峰由对应于以下所示结构的数字标记。参见图5的质谱和表3中通过DB5和VF-WAXms柱分析的萜烯化合物的总结。

图8显示了由表达来自柱状田头菇的TPS基因的大肠杆菌生产的萜烯的GC-MS谱图。代谢物用VF-WAXms柱进行分析。“对照(Ctrl)”是由具有空载体的大肠杆菌菌株产生的挥发性代谢物的GC-MS曲线。由大肠杆菌内源产生的吲哚(*)用作内部参考，以比较萜烯生产的相对量。

图9显示了AAE3_10454的产物的核磁共振(NMR)光谱分析。

图10显示了使用精油作为化学标准品来鉴定所鉴定的萜烯化合物。在该研究中使用VF-WAXms柱。

图11显示了使用精油作为化学标准品来鉴定所鉴定的萜烯化合物。在本研究中使用DB-5ms柱。

图12显示了由AAE3_9164生产的单萜烯的GC-MS谱图。

图13显示了来自四种真菌的已表征的TPS同系物的系统发生学。TPS来自柱状田头菇(AAE3)、灰盖鬼伞(C.cinereus,Cop)、发光类脐菇(O.olearius,Omp)、毛韧革菌(Stereumhirsutum,Stehi1)，以及来自高卢蜜环菌(Armillaria gallica,Pro1)的单一TPS。用于最终比对的序列可见于表1。用正方形(“■”)突出显示的TPS未检测到萜烯产物。

图14显示了用于萜烯分析的不同GC柱的比较。绿花白千层精油(Niaouliessential oil)作为喇叭烯(viridiflorene)6和绿花白千层醇(viridiflorol)7的标准品。如通过来自Santa Cruz生物技术的真实化学标准品(authentic chemical standards)所证实的，在DB-5柱中，绿花白千层醇7具有比喇叭烯6相对更低的信号(图17)。通过真实标准品进一步验证了DB-5和VF-WAXms中绿花白千层醇和石竹烯(caryophyllene，在结构上类似于喇叭烯)的相对强度的差异。

图15显示了来自番茄(Solanum lycopersicum)的AAE3_12839(SEQ ID NO:34)和TPS31(SEQ ID NO:75)的相似性分析。真菌喇叭烯合酶(AAE3_12839)与植物喇叭烯合酶几乎没有同一性和相似性。

图16显示了来自五脉白千层(Melaleuca quinquenervia)的AAE3_13291(SEQ IDNO:32)和MqTPS1(SEQ ID NO:76)的相似性分析。真菌绿花白千层醇合酶(AAE3_12839)与植物绿花白千层醇合酶几乎没有同一性和相似性。

图17显示了所提出的用于形成主要产物的反应机制。FPP电离产生的碳正离子经历两种不同的主环闭合(为1,10或1,11闭环)。重组柱状田头菇TPS产生的主要化合物用数字标记，包括Δ6-原伊鲁烯(protoilludene)1、γ-衣兰油烯(muurolene)2、β-杜松萜烯(cadinene)3、δ-杜松萜烯4、α-衣兰油烯5、喇叭烯6、绿花白千层醇7、δ-杜松醇(cadinol)8和表荜澄茄油烯醇(epicubenol)10。质谱参见图5。

图18显示了在85个担子菌门和239个子囊菌门基因组中鉴定的TPS同系物的系统发生树。(A)显示了所有真菌TPS聚集成7个不同的进化枝。本研究和文献中表征的TPS在图中标记。请参阅表1中的更多信息。特别地，这些TPS包括柱状田头菇TPS(AAE3)、灰盖鬼伞TPS(Cop)、发光类脐菇TPS(Omp)、毛韧革菌TPS(Stehi1)、香灰菌TPS(Hyp)、水稻恶苗病菌TPS(Ffsc4、Ffsc6、STC3和STC5)以及一些马兜铃烯合酶TPS(AtARS和PrARS)。大多数担子菌门TPS(包括所有11种柱状田头菇TPS)在进化枝I、II和III中聚类，但子囊菌门TPS分散于进化枝IV、V、VI和VII中。(B)显示了基于系统发生分析可能的Δ6-原伊鲁烯合酶。在本研究或文献中表征了用圆圈(“●·”)突出显示的TPS。

图19显示了生物信息学引导的预测框架——all-by-all BLAST。使用酶功能主动性(EFI)-酶相似性工具(EST)对1408个假定TPS候选物进行All-by-all BLAST。序列相似性网络(SSN)是通过以比对得分100来将序列过滤为簇而生成的。通过Cytoscape 3.5.1版使用SSN进行可视化。在本研究和文献中，将在此获得的模型与图13中的系统发生树一起使用以基于序列相似性和所表征的TPS来预测TPS功能。

图20显示了假定的真菌芳樟醇/橙花叔醇合酶(FNS)的聚类的预测性实例。基于EFI-EST分析，将一组TPS同系物与AAE3_9435聚类。通过将比对分数设置在80和90之间，选择较小的候选组。

图21显示了假定的真菌芳樟醇/橙花叔醇合酶的验证的预测性实例。选择的假定FNS在工程化大肠杆菌菌株中表达。在聚簇中选择的FNS来自于柱状田头菇(AAE3)、平田头菇(Agrped1)、纹缘盔孢伞(Galma)、砖红韧黑伞(Hypsu1)和粘滑菇(M413)。发现Agrped1_689675是一种新的单萜烯合酶，芳樟醇合酶(FS)，而其他的为双功能FNS。

图22显示了验证的FNS和FS的测序比对。测序比对表明这些TPS的类似位置是107/34＝31％。基于比对，鉴定了可影响FS(Agrped1_689675)活性的关键氨基酸F204。

图23显示了FS Agrped1_689675的工程化结果。三个突变体(F204D、F204G和F204R)具有与野生型酶不同的产物谱。它产生乙酸香叶酯(由美国国家标准技术研究院(NIST)文库预测)和芳樟醇。其他突变体(F204I、F204L和F204V)与野生型Agrped1_689675共享相同的产物(芳樟醇)。

图24显示了假定的真菌绿花白千层醇合酶的聚类和验证的预测性实例。根据图18和19中的系统发生树，15个真菌TPS同系物紧密聚类。将其中的4个(来自弹球菌的Sphst_47084、来自Dendrothele bispora的Denbi1_816208、来自纹缘盔孢伞的Galma_104215和来自Piloderma croceum的Pilcr_825684)在大肠杆菌中重组表达并分析其产物。系统发生分析和EFI-EST分析的预测都非常准确。在本研究中表征了用圆圈(“●”)突出显示的TPS。

图25显示了本研究和文献中表征的TPS的金属结合基序。

图26显示了Agrped1_689675和Agrped1_689675的同系物模型的晶体结构，其中突出显示了底物结合口袋。利用Swiss Model同源建模服务器，以5nx6和5nx5为模板进行比对生成模型。使用PyMol可视化蛋白质模型并与其模板结构比对。

图27显示DXS(PDB ID:2o1s)的晶体结构，其中突出显示了底物和突变的氨基酸。

具体实施方式

在第一方面，本发明涉及包含一种或多种载体的细菌菌株，该载体编码a)产生一种或多种萜烯前体的一种或多种酶，和b)真菌萜烯合酶(FTPS)。

本领域技术人员将理解，上述一种或多种载体包含编码产生一种或多种萜烯前体的一种或多种酶和FTPS的多核苷酸序列。

编码产生一种或多种萜烯前体的一种或多种酶和FTPS的多核苷酸可位于不同的载体上、单一载体上或其组合。在一个实施方案中，编码产生一种或多种萜烯前体的一种或多种酶的多核苷酸位于单一载体中，编码FTPS的多核苷酸位于不同的载体中。

在一个实施方案中，一种或多种载体包含与启动子可操作连接的编码一种或多种酶和FTPS的一种或多种核苷酸序列。

在一些实施方案中，启动子是组成型启动子或诱导型启动子。在一个优选的实施方案中，启动子是诱导型启动子。诱导型启动子的实例包括但不限于T7 RNA聚合酶启动子、araBAD启动子、lac启动子、trp启动子和Tac启动子(ptac)或这些启动子的变体。

在一个优选的实施方案中，诱导型启动子是T7 RNA聚合酶启动子。

在一个实施方案中，产生一种或多种萜烯前体的一种或多种酶是1-脱氧-D-木酮糖5-磷酸(DXP)途径的一部分。本领域技术人员将理解，DXP途径也称为非甲羟戊酸途径、甲羟戊酸非依赖性途径或2-C-甲基-D-赤藓醇4-磷酸/1-脱氧-D-木酮糖5-磷酸(MEP/DOXP)途径。DXP途径将丙酮酸和甘油醛-3-磷酸转化为萜烯前体，且该途径中的酶包括DOXP合酶(DXS)、DXP还原酶(DXR)、2-C-甲基-D-赤藓醇4-磷酸胞苷酰转移酶(IspD)、4-二磷酸胞苷-2-C-甲基-赤藓醇激酶(IspE)、2-C-甲基-D-赤藓醇2,4-环二磷酸合酶(IspF)、HMB-PP合酶(ispG)、HMB-PP还原酶(IspH)和异戊烯基二磷酸异构酶(IDI)。

在一个优选的实施方案中，酶是1-脱氧木酮糖-5-磷酸合酶(DXS)、异戊烯基二磷酸异构酶(IDI)或二者。

在一个实施方案中，DXS包含SEQ ID NO:6所示的氨基酸序列。

在一些实施方案中，DXS可以是基因修饰的。基因修饰可以是在编码DXS的氨基酸序列的一个或多个氨基酸位置处的突变。在一些实例中，突变是氨基酸取代、插入、缺失或其组合。

在一些实施方案中，基因修饰的DXS具有比未修饰的或野生型DXS更高的溶解度。

在一些实施方案中，突变选自由SEQ ID NO:6中的H105T、E210D、Q459L、L415T及其组合组成的组。

在一个优选的实施方案中，突变是H105T。

在另一个优选的实施方案中，突变是E210D、Q459L和L415T。

在一个实施方案中，基因修饰的DXS包含SEQ ID NO:24或SEQ ID NO:25中所示的氨基酸序列。

在一个实施方案中，DXS包含与SEQ ID NO:6、SEQ ID NO:24或SEQ ID NO:25中所示的氨基酸序列具有至少50％、至少60％、至少70％、至少80％、至少90％、至少95％、至少96％、至少97％、至少98％、至少99％或100％同一性的氨基酸序列。

在一个实施方案中，产生一种或多种萜烯前体的一种或多种酶与野生型酶相比表达水平升高。上述一种或多种酶可以是基因修饰的。

在一些实施方案中，本文所述的萜烯前体是焦磷酸法尼酯(FPP)、焦磷酸香叶酯(GPP)、焦磷酸香叶基香叶酯(GGPP)或其组合。

在优选实施方案中，萜烯前体是FPP和/或GPP。

本文所述的细菌菌株包含一种或多种编码真菌萜烯合酶(FTPS)的载体。在一个优选的实施方案中，FTPS是单萜烯合酶或倍半萜烯合酶。在进一步优选的实施方案中，FTPS是芳樟醇合酶(linalool synthase)、橙花叔醇合酶(nerolidol synthase)或芳樟醇和橙花叔醇合酶(FNS)。

在一些实施方案中，所述FTPS分离自柱状田头菇(Agrocybe aegerita)、平田头菇(Agrocybe pediades)、纹缘盔孢伞(Galerina marginata)、砖红韧黑伞(Hypholomasublateritium)、Dendrothele bispora、可可链疫孢荚腐病菌(Moniliophthora roreri)、Piloderma croceum、弹球菌(Sphaerobolus stellatus)、灰盖鬼伞(Coprinopsiscinerea)、发光类脐菇(Omphalotus olearius)、红缘层孔菌(Fomitopsis pinicola)、毛韧革菌(Stereum hirsutum)、禾谷镰刀菌(Fusarium graminearum)、水稻恶苗病菌(Fusariumfujikuroi)、拟枝孢镰刀菌(Fusarium sporotrichioides)、土曲霉(Aspergillusterreus)、娄地青霉(Penicillium roqueforti)、香灰菌(Hypoxylon sp.)、高卢蜜环菌(Armillaria gallica)、葡萄孢菌(Botrytis cinerea)、光轮层炭壳菌(Daldiniaeschscholzii)或其组合。

在一个实施方案中，所述FTPS分离自柱状田头菇(Agrocybe aegerita)、平田头菇(Agrocybe pediades)、纹缘盔孢伞(Galerina marginata)、砖红韧黑伞(Hypholomasublateritium)、粘滑菇(Hebeloma cylindrosporum)或其组合。

在一个优选的实施方案中，所述FTPS分离自柱状田头菇或平田头菇。

在一个实施方案中，FTPS包含选自由SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33和SEQ ID NO:34组成的组的氨基酸序列。

在一些实施方案中，FTPS包含选自由SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39组成的组的核酸序列。

在一个实例中，本文所述的细菌菌株包含的FTPS的表达水平高于野生型FTPS。

在一些实施方案中，FTPS可以是基因修饰的。基因修饰可以是氨基酸取代、插入、缺失、C末端截短、N末端截短或其组合。突变可以是FTPS的UP结构域中的一个或多个突变、FTPS的DW结构域中的一个或多个突变、或二者。本领域技术人员应理解，UP和DW结构域根据真菌菌株而变化。在一个实例中，分离自Agrped1_689675(SEQ ID NO:3)的FTPS的UP结构域的特征在于氨基酸位置1-170。在一个实施方案中，分离自Agrped1_689671(SEQ ID NO:2)的FTPS的UP结构域的特征为氨基酸位置1-169。在又一个实施方案中，分离自Agrped1_689675(SEQ ID NO:3)的FTPS的DW结构域的特征在于氨基酸位置171-325。在又一个实施方案中，分离自Agrped1_689671(SEQ ID NO:2)的FTPS的DW结构域的特征在于氨基酸位置170-324。

在一个优选的实施方案中，突变是SEQ ID NO:3中的F204D、F204G、F204R、F204I、F204L、F204V或其组合。

在一些实施方案中，基因修饰的FTPS包含选自由SEQ ID NO:7、SEQ ID NO:8、SEQID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQID NO:21、SEQ ID NO:22和SEQ ID NO:23的氨基酸序列。

在一些实施方案中，FTPS包含与SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ IDNO:4、SEQ ID NO:5、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ IDNO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ IDNO:23、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ IDNO:31、SEQ ID NO:32、SEQ ID NO:33和SEQ ID NO:34所示的氨基酸序列具有至少80％、至少90％、至少95％、至少96％、至少97％、至少98％、至少99％或100％同一性的氨基酸序列。

在一些实施方案中，细菌菌株是经修饰的或基因修饰的。

在一个实施方案中，本文描述的细菌菌株是大肠杆菌(Escherichia coli)。

在一个方面，本发明涉及基因修饰的1-脱氧木酮糖-5-磷酸合酶(DXS)，其中该基因修饰是在一个或多个氨基酸位置处的突变。在一个实施方案中，本文所述的突变是氨基酸取代或插入或缺失。在又一个实施方案中，突变选自由SEQ ID NO:6中的H105T、E210D、Q459L、L415T及其组合组成的组。

在一个优选的实施方案中，突变是E210D、Q459L和L415T。

在另一个优选的实施方案中，突变是H105T。

在另一方面，本发明涉及包含如SEQ ID NO:24或SEQ ID NO:25所示的氨基酸序列的基因修饰的DXS酶。

在又一个方面，本发明涉及包含氨基酸序列的基因修饰的DXS酶，该氨基酸序列与SEQ ID NO:24或SEQ ID NO:25所示的氨基酸序列具有至少50％、至少60％、至少70％、至少80％、至少90％、至少95％、至少96％、至少97％、至少98％、至少99％或100％的同一性。

在一个方面，本发明涉及基因修饰的真菌萜烯合酶(FTPS)，其中基因修饰是在一个或多个氨基酸位置的突变。

相对于野生型或未修饰的FTPS，基因修饰的FTPS进行了修饰。在一个实施方案中，未修饰的FTPS分离自柱状田头菇(Agrocybe aegerita)、平田头菇(Agrocybe pediades)、纹缘盔孢伞(Galerina marginata)、砖红韧黑伞(Hypholoma sublateritium)、粘滑菇(Hebeloma cylindrosporum)或其组合。

在一个优选的实施方案中，未修饰的FTPS分离自柱状田头菇或平田头菇。

在一些实施方案中，本文所述的突变是氨基酸取代、插入、缺失、C末端截短、N末端截短或其组合。突变可以是FTPS的UP结构域中的一个或多个突变、FTPS的DW结构域中的一个或多个突变、或二者。本领域技术人员应理解，UP和DW结构域根据真菌菌株而变化。在一个实例中，分离自Agrped1_689675(SEQ ID NO:3)的FTPS的UP结构域的特征在于氨基酸位置1-170。在一个实施方案中，分离自Agrped1_689671(SEQ ID NO:2)的FTPS的UP结构域的特征为氨基酸位置1-169。在又一个实施方案中，分离自Agrped1_689675(SEQ ID NO:3)的FTPS的DW结构域的特征在于氨基酸位置171-325。在又一个实施方案中，分离自Agrped1_689671(SEQ ID NO:2)的FTPS的DW结构域的特征在于氨基酸位置170-324。

在一个优选的实施方案中，突变选自由SEQ ID NO:3的F204D、F204G、F204R、F204I、F204L和F204V组成的组。

在一些实施方案中，本文所描述的基因修饰的FTPS是芳樟醇合酶、橙花叔醇合酶或二者。

在一个实施方案中，本发明涉及包含氨基酸序列的基因修饰的FTPS，该氨基酸序列与选自由SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16SEQ ID NO:17、SEQID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22和SEQ ID NO:23组成的组的氨基酸序列具有至少50％、至少60％、至少70％、至少80％、至少90％、至少95％、至少96％、至少97％、至少98％、至少99％或100％的同一性。

在一个方面，本发明涉及生产萜类化合物的方法，包括a)在表达培养基中培养如本文所述的细菌菌株，和b)从所述表达培养基中分离萜类化合物。

在表达培养基中培养细菌菌株将允许表达产生一种或多种萜烯前体的一种或多种酶和表达FTPS。

在另一方面，本发明涉及生产萜类化合物的方法，包括a)在表达培养基中培养包含编码本文所述的基因修饰的FTPS的载体的细菌菌株，和b)从所述表达培养基中分离萜类化合物。

表达培养基可以是支持细菌菌株生长的任何培养基。上述表达培养基可以包含能够在一种或多种载体中诱导上述诱导型启动子的诱导物。表达培养基也可以是自诱导培养基。在一个实例中，自诱导培养基是ZYM5052。在其他实例中，自诱导培养基是溶原性肉汤(LB琼脂)、极品肉汤(TB)或2xPY。

表达培养基可进一步补充球形C18树脂或Ni-次氮基三乙酸树脂。

在一个实施方案中，本文所述的方法还包括从细菌细胞分离FTPS并将分离的FTPS与一种或多种萜烯前体混合以产生萜类化合物的步骤。分离的FTPS可在同一细胞培养容器中或与原始培养物不同的容器中与一种或多种萜烯前体混合。可以使用各种方法分离FTPS。在一些实施方案中，FTPS是通过Ni-次氮基三乙酸树脂、基于柱的方法或同时通过这两种方法分离自细菌细胞。

在另一个实施方案中，在与一种或多种萜烯前体混合之前，将本文所述的分离的FTPS进一步纯化。

在另一个实施方案中，在与一种或多种萜烯前体混合之前，将分离的FTPS进一步与一种或多种另外的酶混合。

在一个实施方案中，本文描述的一种或多种另外的酶是乙酰-CoA乙酰基转移酶、羟甲基戊二酰-CoA合酶(HMGS)、羟甲基戊二酰-CoA合酶还原酶(HMGR)、IDI、甲羟戊酸激酶(MK)、磷酸甲羟戊酸激酶(PMK)或甲羟戊酸二磷酸脱羧酶(MVD1)。在一些实施方案中，乙酰-CoA乙酰基转移酶是PhaA或atoB。在一个实施方案中，萜烯或萜类化合物可以使用本发明的FTPS制备如下：1.使用粗制细胞裂解物，收获仅表达FTPS的细菌细胞并通过冻/融方法和/或超声处理方法裂解。将含有可溶性FTPS的裂解细胞上清液与底物(GPP或FPP)、2.5mMMgCl

在一个实施方案中，本文所述的方法的产物是单萜类化合物、倍半萜类化合物或二者的混合物。在一些实施方案中，所述单萜类化合物选自由β-月桂烯、芳樟醇、乙酸香叶酯及其组合。

在一些实施方案中，所述倍半萜类化合物选自Δ6-原伊鲁烯(Δ6-protoilludene)、α-衣兰油烯(α-muurolene)、γ-衣兰油烯、β-杜松萜烯(β-cadinene)、β-古巴烯(β-copaene)、δ-杜松萜烯(δ-cadinene)、γ-杜松萜烯、表姜烯酮(epizonarene)、α-荜澄茄油烯(α-cubebene)、荜澄茄醇(cubebol)、表荜澄茄油烯醇(epicubenol)、橙花叔醇(nerolidol)、绿花白千层醇(viridiflorol)、喇叭烯(viridiflorene)、α-杜松醇(α-cadinol)、α-表-杜松醇、β-芹子烯(β-selinene)、α-芹子烯、T-依兰油醇(muurolol)、β-榄香烯(β-elemene)、β-古芸烯(β-gurjunene)、吉玛烯A(germacrene A)、吉玛烯D及其组合。

在一个优选的实施方案中，产物是Δ6-原伊鲁烯、芳樟醇、乙酸香叶酯或其组合。

本发明还公开了使用本文所述的FTPS生产一种或多种萜类化合物。

在一些实施方案中，所述一种或多种萜类化合物选自由β-月桂烯、芳樟醇、乙酸香叶酯、Δ6-原伊鲁烯、α-衣兰油烯、γ-衣兰油烯、β-杜松萜烯、β-古巴烯、δ-杜松萜烯、γ-杜松萜烯、表姜烯酮、α-荜澄茄油烯、荜澄茄醇、表荜澄茄油烯醇、橙花叔醇、绿花白千层醇、喇叭烯、α-杜松醇、α-表-杜松醇、β-芹子烯、α-芹子烯、T-衣兰油醇、β-榄香烯、β-古芸烯、吉玛烯A、吉玛烯D、反式-β-罗勒烯、β-荜澄茄油烯、α-isocomene、长叶烯、杜松-3,5-二烯、石竹烯、α-蛇麻烯、荜澄茄油宁烯、卡拉烯、荜澄茄油烯醇、δ-杜松醇、杜松-1(6),4-二烯及其组合组成的组。

在另一个实施方案中，本文所述的一种或多种萜类化合物在体外或体内产生。在一些实施方案中，所述一种或多种萜类化合物在细菌细胞、酵母细胞、植物细胞、动物细胞或真菌细胞中体内产生。在一个实施方案中，细菌细胞是大肠杆菌细胞。在另一个实例中，酵母细胞是酿酒酵母(Saccharomyces cerevisiae)或解脂假丝酵母(Yarrowialipolitica)细胞。

在另一个实施方案中，本文所述的一种或多种萜类化合物在体外产生。

在一些实施方案中，本文所述的FTPS分离自细菌细胞、酵母细胞、植物细胞、动物细胞或真菌细胞，并与一种或多种萜烯前体混合以生产一种或多种萜类化合物。

在另一个实施方案中，在与一种或多种萜烯前体混合之前，将分离的FTPS进一步与一种或多种另外的酶混合。在一个实例中，一种或多种另外的酶是IDI、MK、PMK或PMD。

本发明还公开了一种载体，其包含编码1-脱氧木酮糖-5-磷酸合酶(DXS)酶的多核苷酸序列，其中所述1-脱氧木酮糖-5-磷酸合酶包含SEQ ID NO:6所示的氨基酸序列；或编码如本文所述的基因修饰的1-脱氧木酮糖-5-磷酸合酶(DXS)酶的多核苷酸序列。

在另一个实例中，本发明涉及载体，其包含：编码真菌萜烯合酶(FTPS)的多核苷酸序列，其中所述真菌萜烯合酶(FTPS)包含选自由SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ ID NO:4和SEQ ID NO:5组成的组的氨基酸序列；或编码本文所述的基因修饰的真菌萜烯合酶(FTPS)的多核苷酸序列。

从氨基酸序列生成的多核苷酸序列可以被优化以改善宿主细胞或表达载体中的表达。所述DNA序列可由氨基酸序列产生，以具有优化的密码子适应指数(>0.6)和GC百分比(40-60％)。密码子使用频率表可以基于细菌细胞的菌株，例如，基于大肠杆菌K-12MG1655菌株。在大多数情况下，可以使用基于蒙特卡罗算法(Monte Carlo algorithm)的引导随机方法。然而，可以引入手动调节以去除具有复杂二级结构或重复序列的某些区域。通常应理解，可使用各种密码子优化方法来改善蛋白质或多肽在宿主细胞或表达载体中的表达。

在一个方面，本发明涉及一种由多核苷酸编码的FTPS，该多核苷酸包含选自由SEQID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38和SEQ ID NO:39组成的组的核酸序列。

另一方面，本发明涉及分离的核酸分子，其包含选自由SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38和SEQ ID NO:39组成的组的核酸序列。

在一些实施方案中，核酸序列与选自由SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38和SEQ ID NO:39组成的组的核酸序列具有至少50％、至少60％、至少70％、至少80％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％或100％同一性。

本文公开的细菌菌株可用于表征FTPS。在另一个实施方案中，FTPS可以通过由FTPS产生的产物、FTPS的活性或二者来表征。

在缺乏未在本文中具体公开的任何要素或限制的情况下，可以适当地实施本文示例性描述的本发明。因此，例如，术语“包含(comprising)”、“包括(including)”、“含有(containing)”等应被广义而非限制性地理解。另外，本文使用的术语和表达已经用作描述而非限制的术语，并且使用这些术语和表达并非意图排除所示出和描述的特征的任何等同物或其部分，但是应认识到，在所要求保护的本发明的范围内各种修改是可能的。因此，应当理解，尽管已经通过优选实施方案和可选特征具体公开了本发明，但是本领域技术人员可以对在此公开的本发明实施方案进行修改和变化，并且这些修改和变化被认为是在本发明的范围内。

本文已经广泛地和一般性地描述了本发明。落入一般公开范围内的每个较窄的种类和亚类分组也形成本公开的一部分。这包括带有从该类属中除去任何主题名称的附带条件或否定限制的发明的一般性描述，无论所去除的材料是否在本文中具体叙述。

其他实施方案在所附权利要求书和非限制性实例内。此外，当本发明的特征或方面以马库什组的形式描述时，本领域技术人员将认识到，本发明也由此以马库什组的任何单独的成员或成员的亚组的形式描述。

实验部分

将通过参考具体实施例更详细地进一步描述本发明的非限制性实例和比较例，这些实施例不应被解释为以任何方式限制本发明的范围。

已克隆和功能表征的真菌TPS示于表1中。

表1.公开的功能表征的真菌萜烯合酶

柱状田头菇的栽培及其子实体分析

柱状田头菇野生型菌株AAE-3在改良的结晶皿中于24℃黑暗中生长(图1；下盘：直径70mm，上盘：直径80mm；连接到上盘的玻璃管：外径16mm，内径14mm)，含16mL 1.5％MEA(每升含有15g麦芽提取物和15g琼脂)，并用Parafilm密封。接种后10天，菌丝体覆盖了完整的琼脂表面。除去Parafilm并将样品转移至人工气候室(24℃，95％rH，12/12小时昼/夜节奏)并在玻璃板上再培养16天。使用二乙烯基苯-carboxen-聚二甲基硅氧烷(50/30μm DVB/CAR/PDMS)纤维通过固相微萃取(SPME)收集挥发性有机化合物。从接种后第10天开始，挥发物直接在结晶皿中吸收14h(7/7小时昼/夜循环)。该提取每隔两天进行。对于GC-MS分析，使用配备有VF WAXms色谱柱(Agilent Technologies；30m x 0.25mm,0.25μm)并连接到Agilent 5975C MSD三轴质谱仪(MS)的Agilent Technologies 7890A气相色谱仪(安捷伦科技，瓦尔德布龙，德国)。氦气用作气体载体，流速为1.2ml x min

萜类化合物的气相色谱-质谱分析

在室温下，用DVB/CAR/PDMS(50/30pm二乙烯基苯/carboxen/聚二甲基硅氧烷)纤维(长度1cm；Supelco，施泰因海姆,德国)通过SPME对顶部空间中的挥发性化合物进行了15分钟的采样。将化合物在配有Agilent 7200精确质量四极飞行时间(GC/MS-TOF；安捷伦科技,新加坡)的Agilent 7980B气相色谱的分流/不分流进样口(250℃或150℃；SPME衬管,0.75mm i.d.；Supelco)中解吸1分钟。此外，对于液体培养分析，使用十二烷(20％v/v)提取在大肠杆菌培养物中生产的萜类化合物。将获得的十二烷以1:100稀释在己烷中用于GC-MS分析。GC/MS-TOF配备有VF-WAXms柱(安捷伦科技；30m x 0.25mm i.d.,0.25pm薄膜厚度)或DB-5ms柱(安捷伦科技；30m x 0.25mm i.d.,0.25pm薄膜厚度)，且系统在以下条件下操作：(1)VF-WAXms，以10:1的分流比在分流模式下检测化合物，将GC烘箱温度编程为以10℃×min

萜类化合物的结构鉴定

由电子电离方式获得的质谱通过与美国国家标准技术研究院(NIST)数据库中的萜类化合物的谱图和已发表的萜类谱图进行比较来初步鉴定化合物。此外，通过用C8-C30烷烃混合物的GC-MS校准来鉴定所产生的化合物的Kovats保留指数，并将其与文献中或NIST数据库中公开的保留指数进行比较。只要可能，通过保留时间和质谱与真实标准或具有已知萜烯组合物的精油的比较来验证主要萜烯产物。绿花白千层精油[喇叭烯6(10.1％w/w)、绿花白千层醇7(18.1％w/w)]、香椿树油[α-衣兰油烯5(1％w/w)、δ-杜松萜烯4(11.7％w/w)]、荜澄茄油[吉玛烯D(1％w/w)、γ-衣兰油烯2(4.2％w/w)、β-荜澄茄油烯(4.4％w/w)、荜澄茄醇(15.2％w/w)]、阿米香树油[β-榄香烯(吉玛烯A)(0.1％w/w)、δ-杜松烯醇，0.2％]。此外，通过核磁共振谱进一步证实了Δ6-原伊鲁烯的结构。

柱状田头菇基因组中萜烯合酶及其基因簇的功能注释

使用

萜烯合酶基因在大肠杆菌中的克隆及其表达

通过集成DNA技术合成候选真菌TPS基因，并进行密码子优化以在大肠杆菌中表达。将基因克隆到pET11a载体中，在T7启动子控制下表达。将所得质粒转化到BL 21菌株中，该菌株携带从质粒pACM-T7-dxs-T7-idi-T7-ADS-ispA中重新设计的质粒p15A-cam-T7-dxs-idi。此外，质粒中的dxS突变为SL3或SL5(图2)以提高溶解度和活性。将转化的大肠杆菌细胞的单菌落接种到含有氨苄青霉素(100mg/L)和氯霉素(33mg/L)的4ml ZYM5052自诱导培养基(1％胰蛋白胨，0.5％酵母提取物，25mM Na2HPO4，25mM KH2PO4，50mM NH4Cl，5mMNa2SO4，2mM MgSO4，0.5％甘油，0.05％葡萄糖，0.2％α-乳糖)(Studier，2005)中。在28℃和250rpm在振荡培养箱中培养14小时后，将培养液转移到20mL顶部空间螺旋盖小瓶(Merck)中，并通过SPME在50℃对顶部空间进行采样15分钟。

同源性检索和系统发生树构建

用11种柱状田头菇TPS在担子菌门和子囊菌门基因组中搜索其他真菌TPS，该基因组由联合基因组研究所在真菌基因组程序(http://genome.jgi-psf.org/programs/fungi/index.jsf)下和UniProt数据库中通过基本局部比对搜索工具程序(http://www.uniprot.org/blast/)测序和公布。另外，先前公布的392个担子菌门TPS被并入。人工检查组合的TPS候选物的重复序列、错误的蛋白质预测，例如偏离预期蛋白质长度(200-800aa，除了两个假定的TPS，Disac1_349444和EXIGL_831178)或缺乏保守的金属结合DxxxD和NSE/DTE三联体、或具有预测的额外结构域(例如焦磷酸香叶基香叶酯合酶功能)的不完全序列。在鉴定假定的TPS氨基酸序列后，使用Clustal Omega进行比对，并且使用ClustalOmega或MEGA 7.0.26版用邻接法进行系统发生分析。

通过序列相似性网络分析TPS同系物

通过酶功能主动性-酶相似性工具(EFI-EST)Web工具(http://efi.igb.illinois.edu/efi-est/)分析了经过整理的真菌TPS，以生成序列相似性网络(SSN)。使用开源软件Cytoscape(http://www.cytoscape.org/)可视化生成的SSN。检查生成的SSN对于获得同功能簇(isofunctional cluster)是必要的。基于由EFI-EST产生的SSN和顺序地改变一系列独立于数据库的比对得分，获得一组假定同功能组(putativeisofunctional group，PIG)。比较PIG和传统系统发生树的数据以选择假定的同功能TPS。这里，选择3种新型的TPS(绿花白千层醇合酶AAE3_13291、喇叭烯合酶AAE3_12839和芳樟醇/橙花叔醇合酶AAE3_9435)来探查其他的假定同功能TPS，这些TPS均通过实验进一步验证。

Δ6-原伊鲁烯提取和其结构的NMR验证

将AAE3_10454重组大肠杆菌菌株在200mL的ZYM5052自诱导培养基中培养，该培养基补充有100mg的球形C18树脂(VersaFlash球形C18结合闪光键合硅45-75μm，Sigma-Aldrich)。在28℃和150rpm培养24小时后，通过C18柱(C18 cartridge)手动过滤细胞培养物，随后用去离子水洗涤两次。过滤后，从C18柱除去细胞和液体培养基。用10mL己烷洗脱与C18树脂结合的萜烯化合物。将洗脱的萜烯溶液在4℃蒸发，随后使用配备有z-梯度的5-mmBBI(1H，G-COSY，多重编辑的G-HSQC和G-HMBC光谱)或BBO(13C光谱)探头在具有Cryoprobe的Bruker DRX-400 NMR光谱仪上进行分析。根据残余质子化溶剂信号CHCl3δH 7.24和CDCl3δC 77.23)对光谱进行校准。通过将NMR光谱数据与文献中报道的数据进行比较，萜烯化合物被确认为Δ6-原伊鲁烯。

实施例1

工程化大肠杆菌菌株以进行TPS表征

野生型大肠杆菌BL21产生少量萜烯前体(GPP和FPP)，因此，其不适合作为TPS表征平台。为了提高检测的灵敏度和准确度，DXS和IDI被过表达以提高胞内前体(图3)。与现有方法不同，基于随机诱变和筛选鉴定了两种DXS突变体(SL3和SL5)。如图2所示，与野生型DXS相比，SL3和SL5具有更高的溶解度，因此具有比野生型DXS更高的活性。更重要的是，SL5比野生型DXS具有更高的比活性(图2B)。因此，过表达SL3或SL5的菌株的番茄红素产量高于野生型DXS。这里，使用番茄红素作为指标来证明菌株(SL3和SL5)中的GPP和FPP更高。使用DXS突变体，可以进一步提高细胞平台的检测灵敏度。因此，将大肠杆菌菌株(SL3或SL5)用作表征TPS的平台。图27显示了DXS的晶体结构，其中已经突出了有益突变。这些突变体与通过酶工程方法提高DXS的溶解度有关。配位体焦磷酸盐呈鲑鱼色，且镁呈耐火砖色。

实施例2

柱状田头菇中生产的萜烯的分析

为了获得在柱状田头菇中生产的萜烯的评估，分析了其液体培养物产生的挥发性化合物。隐杯伞素(illudin)前体Δ(6)原伊鲁烯1是柱状田头菇中产生的主要代谢物(图1)。此外，培养26天后，观察到少量的α-ioscomene

实施例3

柱状田头菇的倍半萜烯醇

在柱状田头菇的结实期间，通过GC/MS分析检测出20种假定的萜类化合物，其中试验性鉴定的Δ6-原伊鲁烯

表2柱状田头菇STS基因的详细情况

实施例4

11种预测的倍半萜烯合酶的表征

对所有11种预测的STS进行密码子优化，并将其克隆到pET载体中，该载体被转化到过量生产倍半萜烯的前体法尼基焦磷酸酯(FPP)的工程化的大肠杆菌BL21菌株中。与方法中描述的文献和数据库相比，根据其保留指数(RI)和质谱用井号(#)标记初步鉴定的化合物。

除AAE3_09008和AAE3_05024以外的所有STS(TPS)在相应大肠杆菌克隆的液体培养物中均产生一种或多种倍半萜烯(图7(DB-5ms柱)、图8(VFWAXms柱)和表3)。AAE3_04120和AAE3_10454产生与仅有的产物相同的倍半萜烯。NIST数据库检索以及与先前报道(图5和表3)的真菌倍半萜烯的质谱进行比较揭示了该化合物可能是Δ6-原伊鲁烯1。并通过NMR分析进一步验证了其结构，该NMR分析与先前的报道具有相同的光谱(图9)。Δ6-原伊鲁烯是显示抗肿瘤和抗微生物作用的隐杯伞素的前体。到目前为止，已经报道了来自3种真菌物种的6种Δ6-原伊鲁烯合酶，来自发光类脐菇的Omp6和Omp7，来自高卢蜜环菌的Prol和来自毛韧革菌的Stehi1_25180、Stehi1_64702和Stehi1_73029。有趣的是，AAE3_04120和AAE3_10454与系统发生聚类中报道的6种合酶形成紧密相关的亚组(图1)，表明6-原伊鲁烯合酶在不同真菌物种之间高度相关。

表3.本研究中TPS的萜烯产物

表达AAE3_0444(SEQ ID NO:27)和AAE3_6743(SEQ ID NO:29)的大肠杆菌菌株生产几种倍半萜烯化合物(图7、图8和表3)。γ-衣兰油烯2和β-杜松萜烯

在表达AAE3_09164(SEQ ID NO:30)的大肠杆菌培养物中检测到多种倍半萜烯(图7、图8和表3)。其中以δ-杜松萜烯4(37％)为主要产物，同时还包括γ-衣兰油烯2(15％)、表姜烯酮

表达AAE3_13190(SEQ ID NO:33)的大肠杆菌菌株生产四种主要产物，α-衣兰油烯5(32％)和γ-衣兰油烯2(22％)、δ-杜松萜烯4(24％)和δ-杜松醇

AAE3_12839(SEQ ID NO:34)的主要产物是喇叭烯6。相反，表达AAE3_13291(SEQID NO:32)的大肠杆菌菌株生产绿花白千层醇7作为主要产物(绿花白千层醇7和喇叭烯6被绿花白千层精油所证实，图14)，其中有少量喇叭烯6(8.6％，表3和图8中VFWAXms)。这里，使用VFWAXms柱而不是DB5柱的数据来定量绿花白千层醇7，因为DB5中绿花白千层醇7的定量不准确，信号显著低于6(图7和表3)。据我们所知，在真菌中没有报道过喇叭烯合酶或绿花白千层醇合酶。即使在植物中，从番茄(Solanum lycopersicum)和烟草(Nicotianatabacum)(普通烟草)中也仅鉴定出了6种喇叭烯合酶。AAE3_12839和番茄喇叭烯合酶的比对表明存在有限的序列相似性(图15)。类似地，AAE3_13291与来自五脉白千层(Melaleucaquinquenervia)的绿花白千层醇合酶共享仅11％的同一性和小于30％的相似性，其是迄今为止报道的仅有的绿花白千层醇合酶(图16)。

此外，柱状田头菇中鉴定的TPS与灰盖鬼伞和发光类脐菇中的TPS共享相同的第一环化步骤。对于Δ-6-原伊鲁烯(AAE3_4120(SEQ ID NO:26)和AAE3_10454(SEQ ID NO:31)),喇叭烯合酶(AAE3_12839(SEQ ID NO:34))和绿花白千层醇合酶(AAE3_13291(SEQ IDNO:32))，它们都通过FPP的1,11环化反应形成三环倍半萜烯(图17)。相反，其他TPS(AAE3J34444(SEQ ID NO:27),AAE3_6595(SEQ ID NO:28)、AAE3_9164(SEQ ID NO:30)、AAE3_13190(SEQ ID NO:33)和AAE3_6743(SEQ ID NO:29))优先催化FPP的1,10环化以形成双环倍半萜烯。

实施例5

TPS功能研究的真菌基因组分析

图7和13中的结果巩固了某些类型的真菌TPS具有由相同产物强化的高度保守序列，例如八种表征的Δ6-原伊鲁烯合酶和四种表征的δ-杜松萜烯合酶。因此，系统发生分析提供了一种预测性框架，以鉴定具有新的或相似功能的新的萜烯合酶。模型的预测精度随着实验表征的TPS数量的累积而增加。以前，通过生物信息学分析正确预测和验证了三种Δ6-原伊鲁烯合酶(来自毛韧革菌Stehi1_25180、Stehi1_64702和Stehi1_73029)。自此，许多新真菌物种的基因组已被测序，但它们的TPS基因尚未被研究。这里，目的是建立新的预测框架，用于用新的表征的柱状田头菇TPS和先前研究的真菌TPS对未表征的真菌TPS进行功能研究。通过在联合基因组研究所((JGI,http://genome.jgi-psf.org/programs/fungi/index.jsf))和通用蛋白质资源(UniProt,http://www.uniprot.org/)中的真菌基因组数据库中进行BLAST搜索，发现了大约2,000个假定的TPS基因。在一系列规整后(如以上方法中所述)，从85个担子菌门和239个子囊菌门基因组获得总共1,408个假定的TPS(表4)。平均而言，担子菌门每个基因组平均具有10-15个TPS(来自84个担子菌门的800个TPS)，但是约80％的子囊菌门每个基因组仅具有1-3个TPS(来自236个子囊菌门的594个TPS)。

表4.本研究中关于1408个假定真菌TPS的信息

使用1408个TPS，构建具有7个主要不同TPS进化枝的系统发生树(图18)，并使用酶功能主动性(EFI)-酶相似性工具(EST)进行all-by-all BLAST分析(图19)。所有的进化枝具有至少两种表征的TPS酶。产生一系列衣兰油烯和杜松萜烯化合物(例如Omp1-3、Cop1-3、AAE3_13190和AAE3_6595)的混杂TPS在进化枝I中聚类(图18)。有趣的是，在进化枝I中所有表征的TPS催化FPP的1,10环化(图17)。所有8种表征的Δ6-原伊鲁烯(FPP的1,11环化，图17)合酶，连同其他32种假定的TPS，紧密地分组在进化枝II中。此外，四种TPS AAE3_9008、AAE3_6743、AAE3_0444、AAE3_5024在进化枝II中紧密地分隔，其中多种产物包括衣兰油烯和杜松萜烯(FPP的1,10环化，图17)。以杜松萜烯为主要产物的TPS(Cop4、Stehi1_128017、Omp4-5a,b和AAE3_9164)在进化枝III中聚类在一起。喇叭烯和绿花白千层醇合酶(AAE3_12839和AAE3_13291，FPP的1,11环化，图17)也在进化枝III中，但与碳二烯(cadiene)合酶不同。Hyp1、2和5松散地分散在进化枝IV中，因为它们具有不同的产物和不同的催化机理。Hyp1产生线性萜橙花叔醇，但Hyp2和Hyp5分别催化杜松萜烯和布藜烯(FPP的1,10环化)。Ffsc4(Koraiol，1,11环化)和负责FPP(Omp8-10、Cop6和FsTDS(单端孢霉烯等))的1,6或1,7环化的一些TPS在进化枝V中聚类。此外，Hyp4(未知的倍半萜烯产物)和单萜烯合酶Hyp3(1,8-桉叶素)在进化枝V中分隔。在进化枝VI中，仅表征了两种马兜铃烯(FPP的1,10环化)合酶(AtARS(Cane和Kang，2000)和PrARS(Hohn和Plattner，1989)。最后，一些具有不同环化机制的特征化TPS，包括STC3((+)-雅榄蓝烯，FPP的1,10环化)、STC5((-)-愈创木-6,10(14)-二烯，FPP的1,10环化)、BcBOT2(presilphiperfolan-8β-醇，FPP的1,11环化)和Ffsc6((-)-a-菖蒲烯醇，FPP的1,6环化)，分散在进化枝VII中。总之，大多数担子菌门TPS(包括所有11种柱状田头菇TPS)在进化枝I、II和III中分组，但子囊菌门TPS分散于进化枝IV、V、VI和VII中。担子菌门TPS，特别是紧密聚类的TPS，在每个进化枝中通常共享类似的环化机制。相反，相同进化枝中的子囊菌门TPS可具有多种环化机制。

实施例6

揭示其他真菌绿花白千层醇合酶的预测性框架

通过研究TPS产物获得的知识和每个不同进化枝中的序列保守性为不同活性的机理理解提供了有价值的基础。并且它可以用于工程改造和设计更有效的酶，以及探测和甚至预测未知TPS的功能。为验证该框架的预测能力，对其他物种中的绿花白千层醇合酶进行了鉴定。选择绿花白千层醇合酶的原因是在所有种类的物种中仅报道了一种类型的植物绿花白千层醇合酶。通过图18中的系统发生树和图19中的all-by-all BLAST分析，15个真菌TPS同系物紧密聚类。将其中的4个(来自弹球菌的Sphst_47084、来自Dendrothele bispora的Denbi1_816208、来自纹缘盔孢伞的Galma_104215和来自Piloderma croceum的Pilcr_825684)在大肠杆菌中重组表达并分析其产物。本研究中表征了以圆圈(“●”)突出显示的TPS。其中，4个TPS(来自弹球菌的Sphst_47084、来自Dendrothele bispora的Denbi1_816208、来自纹缘盔孢伞的Galma_104215和来自Piloderma croceum的Pilcr_825684)被克隆并在底盘菌株(chassis strain)中表达。表达与AAE3_13291最密切相关的Sphst_47084和Denbi1_816208的大肠杆菌培养物生产与AAE3_13291相同的产物，如绿花白千层醇7(～90％)和喇叭烯6(～10％)(图24)。有趣的是，Galma_104215的主要产物被初步鉴定为β-古芸烯，一种结构类似于喇叭烯的化合物(图24)。然而，表达Pilcr_825684的细胞生产作为主要产物的γ-杜松萜烯和少量的倍半萜烯，包括喇叭烯。

结果表明，该系统发生树可用于功能相似的新型TPS的鉴定。

实施例7

真菌芳樟醇和橙花叔醇合酶(LNS)的预测与验证

从AAE3_9435的序列开始，通过在联合基因组研究所(JGI,http://jgi.doe.gov/fungi)和通用蛋白质资源(UniProt,http://www.uniprot.org/)的数据库中进行BLAST搜索，可以鉴定出不同真菌物种中的其他NLS。进行EFI-EST分析，并显示一组TPS同系物与AAE3_9435聚类。通过将比对分数设置在80和90之间，选择较小的候选组。使用图20中所选择的TPS聚簇。聚焦比对表明11种真菌TPS与AAE3_9435紧密聚类，包括来自柱状田头菇的两种其他TPS，AAE3_05024和AAE3_04444(SEQ ID NO：27)；来自平田头菇的三种TPS(Agrped1_689671(SEQ ID NO:2)、Agrped1_689675(SEQ ID NO:3)、Agrped1_820868)；来自纹缘盔孢伞的三种TPS(Galma_223690(SEQ ID NO:4)、Galma_266794(SEQ ID NO:77)、Galma_63556)；来自砖红韧黑伞的两种TPS(Hypsu1_148365、Hypsu1_148385(SEQ ID NO:5))；以及来自粘滑菇的M413_27416(图20)。在我们以前的研究中表征的，与AAE3_9435最密切相关的TPS，AAE3_05024，似乎是假基因。AAE3_04444的主要产物为γ-衣兰油烯(33％)和β-杜松萜烯(21％)。图21中的其他TPS未被功能性注释。作为概念的证明，从九个未表征的TPS中选择五个测试它们的功能。当在过量产生IPP和DMAPP的大肠杆菌菌株中表达时，所有五种TPS生产至少一种萜烯。在表达Agrped1_689675的大肠杆菌菌株中，在培养物的顶部空间中仅发现芳樟醇。与AAE3_9435类似，Ageped1_689671、Galma_223690和Hypsu1_148385显示产生橙花叔醇和芳樟醇的双功能NLS功能(图21B)。Galma_266794克隆导致作为主要产物的倍半萜烯吉玛烯D(62％，经毕澄茄精油验证)和一些其他倍半萜烯，包括δ-杜松萜烯(17％)、γ-衣兰油烯(8％)，但没有单萜烯。由于大肠杆菌菌株中缺乏GPP合酶，胞内FPP的浓度远高于胞内GPP的浓度。因此，所有四种TPS(AAE3_9435、Agrped1_689671、Galma_223690和Hypsu1_148385)生产橙花叔醇作为主要产物(88-96％)和芳樟醇作为次要产物(2-10％，图21)。然而，当在相同的大肠杆菌中表达时，Agrped1_689675仅生产芳樟醇，但不产生倍半萜烯。数据表明Agrped1_689675是专一单萜烯合酶，没有倍半萜烯合酶活性，因此被命名为‘Ape_LS'。有趣的是，尽管具有不同的产物，所有六个TPS在金属结合基序中共享一些非常保守的区域(例如‘DEYTD'和‘NDMHSYxxE’区域)。图25显示了倍半萜烯和单萜烯合酶的保守区。2个保守区分别为DD(E/N/Y/S)XXD和NDSE。这两个保守结构域是重要的萜烯合酶同系物的预筛选。那些缺失或结构域不完整的同系物通常没有活性，因此在我们的筛选过程中被排除在外。

实施例8

使LS突变以获得不同的功能

作为专一单萜烯合酶，假设Agrped1_689675(SEQ ID NO:3)的点突变可以改变其功能和产物。为了检验此假设，突出显示了Agrped1_689675和其他位置不同的几个位置(图22)。图26中的晶体结构用于指导工程化Agrped_689675。在不同的氨基酸中，选择F204作为第一突变。发现6个突变体中的3个(F204D、F204G和F204R)具有不同的产物谱。不同于野生型仅产生芳樟醇，它们产生乙酸香叶酯(由NIST文库预测)和芳樟醇(图23)，而其他三种突变体(F204I、F204F和F204V)对酶活性和功能没有显著影响。更有趣的是，乙酸香叶酯的产生与芳樟醇的产生逆相关(图23C)。

基于棒状链霉菌(Streptomyces clavuligerus)的1,8-桉叶素合酶(PDB ID:5nx5,5nx6)的结构，构建了Agr1(Agrped1_689675)和Agr3(Agrped1_689671)的同源模型。通过PyMOL软件v2.1.1测定在离底物

序列表的总结可见于表5。

表5序列表的总结

前述实施例是为了说明本发明的目的而提出的，并且不应被解释为对本发明的范围施加任何限制。很明显，在不偏离本发明的原理的情况下，可以对上述和实施例中说明的本发明的具体实施方案进行许多修改和改变。所有这些修改和改变都包括在本申请中。

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<212> PRT

<213> 大肠杆菌

<400> 6

Met Ser Phe Asp Ile Ala Lys Tyr Pro Thr Leu Ala Leu Val Asp Ser

1 5 10 15

Thr Gln Glu Leu Arg Leu Leu Pro Lys Glu Ser Leu Pro Lys Leu Cys

20 25 30

Asp Glu Leu Arg Arg Tyr Leu Leu Asp Ser Val Ser Arg Ser Ser Gly

35 40 45

His Phe Ala Ser Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His

50 55 60

Tyr Val Tyr Asn Thr Pro Phe Asp Gln Leu Ile Trp Asp Val Gly His

65 70 75 80

Gln Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Arg Asp Lys Ile Gly

85 90 95

Thr Ile Arg Gln Lys Gly Gly Leu His Pro Phe Pro Trp Arg Gly Glu

100 105 110

Ser Glu Tyr Asp Val Leu Ser Val Gly His Ser Ser Thr Ser Ile Ser

115 120 125

Ala Gly Ile Gly Ile Ala Val Ala Ala Glu Lys Glu Gly Lys Asn Arg

130 135 140

Arg Thr Val Cys Val Ile Gly Asp Gly Ala Ile Thr Ala Gly Met Ala

145 150 155 160

Phe Glu Ala Met Asn His Ala Gly Asp Ile Arg Pro Asp Met Leu Val

165 170 175

Ile Leu Asn Asp Asn Glu Met Ser Ile Ser Glu Asn Val Gly Ala Leu

180 185 190

Asn Asn His Leu Ala Gln Leu Leu Ser Gly Lys Leu Tyr Ser Ser Leu

195 200 205

Arg Glu Gly Gly Lys Lys Val Phe Ser Gly Val Pro Pro Ile Lys Glu

210 215 220

Leu Leu Lys Arg Thr Glu Glu His Ile Lys Gly Met Val Val Pro Gly

225 230 235 240

Thr Leu Phe Glu Glu Leu Gly Phe Asn Tyr Ile Gly Pro Val Asp Gly

245 250 255

His Asp Val Leu Gly Leu Ile Thr Thr Leu Lys Asn Met Arg Asp Leu

260 265 270

Lys Gly Pro Gln Phe Leu His Ile Met Thr Lys Lys Gly Arg Gly Tyr

275 280 285

Glu Pro Ala Glu Lys Asp Pro Ile Thr Phe His Ala Val Pro Lys Phe

290 295 300

Asp Pro Ser Ser Gly Cys Leu Pro Lys Ser Ser Gly Gly Leu Pro Ser

305 310 315 320

Tyr Ser Lys Ile Phe Gly Asp Trp Leu Cys Glu Thr Ala Ala Lys Asp

325 330 335

Asn Lys Leu Met Ala Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Met

340 345 350

Val Glu Phe Ser Arg Lys Phe Pro Asp Arg Tyr Phe Asp Val Ala Ile

355 360 365

Ala Glu Gln His Ala Val Thr Phe Ala Ala Gly Leu Ala Ile Gly Gly

370 375 380

Tyr Lys Pro Ile Val Ala Ile Tyr Ser Thr Phe Leu Gln Arg Ala Tyr

385 390 395 400

Asp Gln Val Leu His Asp Val Ala Ile Gln Lys Leu Pro Val Leu Phe

405 410 415

Ala Ile Asp Arg Ala Gly Ile Val Gly Ala Asp Gly Gln Thr His Gln

420 425 430

Gly Ala Phe Asp Leu Ser Tyr Leu Arg Cys Ile Pro Glu Met Val Ile

435 440 445

Met Thr Pro Ser Asp Glu Asn Glu Cys Arg Gln Met Leu Tyr Thr Gly

450 455 460

Tyr His Tyr Asn Asp Gly Pro Ser Ala Val Arg Tyr Pro Arg Gly Asn

465 470 475 480

Ala Val Gly Val Glu Leu Thr Pro Leu Glu Lys Leu Pro Ile Gly Lys

485 490 495

Gly Ile Val Lys Arg Arg Gly Glu Lys Leu Ala Ile Leu Asn Phe Gly

500 505 510

Thr Leu Met Pro Glu Ala Ala Lys Val Ala Glu Ser Leu Asn Ala Thr

515 520 525

Leu Val Asp Met Arg Phe Val Lys Pro Leu Asp Glu Ala Leu Ile Leu

530 535 540

Glu Met Ala Ala Ser His Glu Ala Leu Val Thr Val Glu Glu Asn Ala

545 550 555 560

Ile Met Gly Gly Ala Gly Ser Gly Val Asn Glu Val Leu Met Ala His

565 570 575

Arg Lys Pro Val Pro Val Leu Asn Ile Gly Leu Pro Asp Phe Phe Ile

580 585 590

Pro Gln Gly Thr Gln Glu Glu Met Arg Ala Glu Leu Gly Leu Asp Ala

595 600 605

Ala Gly Met Glu Ala Lys Ile Lys Ala Trp Leu Ala

610 615 620

<210> 7

<211> 336

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut1

<400> 7

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Phe Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

<210> 8

<211> 325

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut2

<400> 8

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Phe Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu

325

<210> 9

<211> 303

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut3

<400> 9

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Phe Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser

290 295 300

<210> 10

<211> 332

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut4

<400> 10

Met Trp Pro Trp Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln

1 5 10 15

Asp Glu Ala Asn Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu

20 25 30

Gln Phe Glu Lys Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu

35 40 45

Val Gly Pro Leu Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu

50 55 60

Met Asn Phe Tyr Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala

65 70 75 80

Asp Glu Ala Lys Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His

85 90 95

Thr Asp Lys Pro Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe

100 105 110

Phe Glu Arg Thr Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu

115 120 125

Gln Phe Ile Ala Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu

130 135 140

Ala Asp Asp Arg Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe

145 150 155 160

Ile Leu Arg Arg Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe

165 170 175

Gly Leu Gly Leu Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Phe

180 185 190

Ile Ser Met Thr Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp

195 200 205

Met His Ser Tyr Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn

210 215 220

Val Ile Thr Ala Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala

225 230 235 240

Leu Tyr Trp Leu Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile

245 250 255

Ser Asp Arg Lys Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala

260 265 270

Val Glu Gln Tyr Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp

275 280 285

Ala Trp Ser Tyr Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu

290 295 300

Ile Gln Lys Thr Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr

305 310 315 320

Val Thr Lys Glu Gln Leu Gln Val Ser Met Lys Ala

325 330

<210> 11

<211> 316

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut5

<400> 11

Met Glu Ala Asn Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu

1 5 10 15

Gln Phe Glu Lys Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu

20 25 30

Val Gly Pro Leu Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu

35 40 45

Met Asn Phe Tyr Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala

50 55 60

Asp Glu Ala Lys Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His

65 70 75 80

Thr Asp Lys Pro Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe

85 90 95

Phe Glu Arg Thr Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu

100 105 110

Gln Phe Ile Ala Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu

115 120 125

Ala Asp Asp Arg Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe

130 135 140

Ile Leu Arg Arg Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe

145 150 155 160

Gly Leu Gly Leu Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Phe

165 170 175

Ile Ser Met Thr Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp

180 185 190

Met His Ser Tyr Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn

195 200 205

Val Ile Thr Ala Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala

210 215 220

Leu Tyr Trp Leu Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile

225 230 235 240

Ser Asp Arg Lys Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala

245 250 255

Val Glu Gln Tyr Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp

260 265 270

Ala Trp Ser Tyr Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu

275 280 285

Ile Gln Lys Thr Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr

290 295 300

Val Thr Lys Glu Gln Leu Gln Val Ser Met Lys Ala

305 310 315

<210> 12

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut6

<400> 12

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Gly Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 13

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut7

<400> 13

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Val Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 14

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut8

<400> 14

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Ile Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 15

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut9

<400> 15

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Asp Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 16

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut10

<400> 16

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Leu Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 17

<211> 344

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut11

<400> 17

Met Ser Ser Gln Ile Tyr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Lys Val Arg Asn Pro Leu Leu Gln Glu Val Gln Asp Glu Ala Asn

20 25 30

Glu Trp Val Lys Ser Phe Val Leu Phe Glu Pro Glu Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly Ala Leu Val Gly Pro Leu

50 55 60

Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala Ser Ala Asp Glu Ala Lys

85 90 95

Val Ile Ala Arg Asp Val Met Glu Ser Phe Arg His Thr Asp Lys Pro

100 105 110

Ser His Asn Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Glu Arg Thr

115 120 125

Ile Asn Thr Val Gly Asn Asp Pro Thr Gly Ile Glu Gln Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Ala Ser Gly His Ile Arg Ser Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu

180 185 190

Asn Ile Pro Lys Glu Val Phe Ala His Pro Met Arg Ile Ser Met Thr

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr

210 215 220

Asn Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr

275 280 285

Phe Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr

305 310 315 320

Arg Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu

325 330 335

Gln Leu Gln Val Ser Met Lys Ala

340

<210> 18

<211> 331

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut12

<400> 18

Met His His His His His His Ser Ser Gln Ile Tyr Ile Pro Asp Leu

1 5 10 15

Leu Ile Thr Trp Pro Trp Gln Lys Val Arg Asn Pro Leu Leu Gln Glu

20 25 30

Val Gln Asp Glu Ala Asn Glu Trp Val Lys Ser Phe Val Leu Phe Glu

35 40 45

Pro Glu Gln Phe Glu Lys Phe Lys Ala Cys Asp Phe Asn Leu Leu Gly

50 55 60

Ala Leu Val Gly Pro Leu Gly Thr Lys Glu Glu Leu Arg Ile Ser Cys

65 70 75 80

Asp Leu Met Asn Phe Tyr Phe Ala Phe Asp Glu Tyr Thr Asp Leu Ala

85 90 95

Ser Ala Asp Glu Ala Lys Val Ile Ala Arg Asp Val Met Glu Ser Phe

100 105 110

Arg His Thr Asp Lys Pro Ser His Asn Lys Ile Thr Glu Met Ala Arg

115 120 125

Gln Phe Phe Glu Arg Thr Ile Asn Thr Val Gly Asn Asp Pro Thr Gly

130 135 140

Ile Glu Gln Phe Ile Ala Asp Phe Asp Ala Tyr Thr Thr Ser Ile Ile

145 150 155 160

Gln Glu Ala Asp Asp Arg Ala Ser Gly His Ile Arg Ser Val Glu Asp

165 170 175

Tyr Phe Thr Leu Arg Arg Asp Thr Cys Gly Ala Lys Pro Ser Phe Ser

180 185 190

Phe Phe Gly Leu Gly Leu Asn Ile Pro Asp Glu Val Phe His His Pro

195 200 205

Val Val Ile Ser Met Ile Glu Gly Ala Thr Asp Leu Ile Ala Val Thr

210 215 220

Asn Asp Met His Ser Tyr Asn Leu Glu His Ser Arg Gly Leu Asp Gly

225 230 235 240

His Asn Val Ile Thr Ala Ile Met His Glu Tyr Gln Leu Asp Leu Gln

245 250 255

Gly Ala Leu Tyr Trp Leu Ser Gly Tyr Ala Thr His Thr Ile Ala Asn

260 265 270

Phe Leu Ser Asn Arg Arg Asn Leu Pro Ser Trp Gly Pro Ala Val Asp

275 280 285

Lys Ala Val Glu Glu Phe Phe Asp Arg Val Gly Arg Cys Val Arg Gly

290 295 300

Tyr Asp Ala Trp Ser Tyr Glu Thr Lys Arg Tyr Tyr Gly Lys Asn Gly

305 310 315 320

Leu Gln Val Gln Lys Thr Arg Arg Ile Thr Leu

325 330

<210> 19

<211> 343

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689675_mut13

<400> 19

Met Ser Gln Phe Ile Ile Pro Asp Leu Leu Ser Thr Trp Pro Trp Gln

1 5 10 15

Arg Val Ser Asn Pro Met Trp Arg Glu Ile Asp Glu Glu Ala Asn Ala

20 25 30

Trp Val Gln Ser Phe Asp Leu Phe Glu Pro Ser Gln Phe Glu Lys Phe

35 40 45

Lys Met Cys Asp Phe Asn Leu Leu Gly Ser Met Ile Gly Thr Val Glu

50 55 60

Thr Lys Asp His Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr Phe

65 70 75 80

Ala Phe Asp Glu Tyr Thr Asp Met Ala Ser Lys Asp Glu Ala Arg Lys

85 90 95

Ile Ala Arg Asp Val Met Asp Ala Phe Arg Asn Thr Glu Lys Pro Ala

100 105 110

His Asn Gln Ile Thr Glu Met Ala Arg Gln Phe Phe Lys Arg Thr Ile

115 120 125

Asp Thr Val Gly Lys Asp Leu Pro Gly Val Glu Arg Phe Ile Ala Asp

130 135 140

Phe Asp Ala Tyr Thr His Ser Val Ile Gln Glu Ala Asp Asp Arg Ala

145 150 155 160

Thr Gly His Ile Arg Ser Val Asn Asp Tyr Phe Ile Leu Arg Arg Asp

165 170 175

Thr Cys Gly Gly Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu Asn

180 185 190

Ile Pro Lys Glu Val Phe Ala His Pro Met Phe Ile Ser Met Thr Glu

195 200 205

Ser Ala Thr Asp Leu Ile Ala Ile Thr Asn Asp Met His Ser Tyr Asn

210 215 220

Leu Glu Gln Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala Ile

225 230 235 240

Met His Glu Tyr Lys Ile Asn Leu Gln Gly Ala Leu Tyr Trp Leu Ser

245 250 255

Gly Tyr Ala Thr Lys Thr Ile Ala Lys Phe Ile Ser Asp Arg Lys Asn

260 265 270

Leu Pro Ser Trp Gly Pro Val Val Asp Arg Ala Val Glu Gln Tyr Phe

275 280 285

Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr Glu

290 295 300

Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Glu Ile Gln Lys Thr Arg

305 310 315 320

Gln Ile Thr Leu Arg Pro Leu Asp Pro Ala Tyr Val Thr Lys Glu Gln

325 330 335

Leu Gln Val Ser Met Lys Ala

340

<210> 20

<211> 356

<212> PRT

<213> 人工序列

<220>

<223> AAE3_109435_mut1

<400> 20

Met His Ser Ser Thr Gln Leu Phe Thr His Ser Pro Thr Lys Met Gln

1 5 10 15

Ile Ile Leu Pro Asp Leu Leu Cys Ser Trp Gly Tyr Lys Gly Phe Leu

20 25 30

Asn Pro His Tyr Glu Gly Ala Lys Ala Glu Ser Asn Thr Trp Ile Arg

35 40 45

Pro Leu Val Val Lys Leu Phe Asp Glu Arg Gly Gln Lys Ala Phe Ala

50 55 60

Lys Asp Tyr Thr Gly Leu Leu Ala Ser Met Thr Tyr Pro His His Asn

65 70 75 80

Lys Glu Phe Leu Cys Val Ala Cys Asp Met Met Asn Leu Phe Phe Val

85 90 95

Tyr Asp Glu Tyr Thr Asp Ile Thr Pro Pro Glu Thr Ala Gln Arg Leu

100 105 110

Ala Lys Ile Val Val Asn Ala Met Arg Asn Pro Asp Glu Ile Ala Ala

115 120 125

Leu Gly Glu Asp Ser Ile Gly Thr Met Thr Lys Gln Phe Trp Arg Arg

130 135 140

Ala Met Thr Leu Leu Pro Pro Asn Gly Cys Asn Ser Glu Ser Cys Ile

145 150 155 160

Gln His Phe Ile Asp Tyr Thr Glu Glu Tyr Leu Thr Ala Val Thr Arg

165 170 175

Glu Ala Cys Asp Arg Ser Ser Gly Ser Val His Ala Val Lys Asp Tyr

180 185 190

Leu Ala Met Arg Arg Ala Thr Ser Gly Ala Gly Leu Met Val Gly Leu

195 200 205

Leu Glu Phe Gly Leu Asp Leu Pro Glu Glu Val Met Lys His Glu Val

210 215 220

Ile Gln Glu Leu Ser Thr Gly Ala Ile Asp Met Tyr Cys Leu Leu Asn

225 230 235 240

Asp Met His Ser Tyr Ala Ser Glu Leu Ser Ser Gly Gln Ala Ser His

245 250 255

Asn Val Ile Thr Val Val Met His Glu Arg Asn Leu Ser Leu Gln Glu

260 265 270

Ala Phe Asp Trp Leu Ala Ser Tyr Ala Ala Gly Val Val Lys Gly Phe

275 280 285

Lys Thr Asn Leu Asn Arg Val Pro Ser Phe Ser Glu Leu Glu Asp His

290 295 300

Ser Leu His Gly Glu Gly Met Leu Arg Asp Arg Ile Gln Arg Tyr Ile

305 310 315 320

Asp Gly Leu Gly Gln Ala Val Arg Ala Glu Asp Asp Trp Ala Phe Glu

325 330 335

Thr Thr Arg Tyr His Gly Gln Asn Gly Pro Gln Ile Lys Leu Thr Arg

340 345 350

Met Leu Thr Ile

355

<210> 21

<211> 324

<212> PRT

<213> 人工序列

<220>

<223> Agrped1_689671_mut1

<400> 21

Met Ser Gln Phe Ile Ile Pro Asp Leu Leu Ser Thr Trp Pro Trp Gln

1 5 10 15

Arg Val Ser Asn Pro Met Trp Arg Glu Ile Asp Glu Glu Ala Asn Ala

20 25 30

Trp Val Gln Ser Phe Asp Leu Phe Glu Pro Ser Gln Phe Glu Lys Phe

35 40 45

Lys Met Cys Asp Phe Asn Leu Leu Gly Ser Met Ile Gly Thr Val Glu

50 55 60

Thr Lys Asp His Leu Arg Ile Ser Cys Asp Leu Met Asn Phe Tyr Phe

65 70 75 80

Ala Phe Asp Glu Tyr Thr Asp Met Ala Ser Lys Asp Glu Ala Arg Lys

85 90 95

Ile Ala Arg Asp Val Met Asp Ala Phe Arg Asn Thr Glu Lys Pro Ala

100 105 110

His Asn Gln Ile Thr Glu Met Ala Arg Gln Phe Phe Lys Arg Thr Ile

115 120 125

Asp Thr Val Gly Lys Asp Leu Pro Gly Val Glu Arg Phe Ile Ala Asp

130 135 140

Phe Asp Ala Tyr Thr His Ser Val Ile Gln Glu Ala Asp Asp Arg Ala

145 150 155 160

Thr Gly His Ile Arg Ser Val Asn Asp Tyr Phe Thr Leu Arg Arg Asp

165 170 175

Thr Cys Gly Ala Lys Pro Ser Phe Ser Phe Phe Gly Leu Gly Leu Asn

180 185 190

Ile Pro Asp Glu Val Phe His His Pro Val Val Ile Ser Met Ile Glu

195 200 205

Gly Ala Thr Asp Leu Ile Ala Val Thr Asn Asp Met His Ser Tyr Asn

210 215 220

Leu Glu His Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala Ile

225 230 235 240

Met His Glu Tyr Gln Leu Asp Leu Gln Gly Ala Leu Tyr Trp Leu Ser

245 250 255

Gly Tyr Ala Thr His Thr Ile Ala Asn Phe Leu Ser Asn Arg Arg Asn

260 265 270

Leu Pro Ser Trp Gly Pro Ala Val Asp Lys Ala Val Glu Glu Phe Phe

275 280 285

Asp Arg Val Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr Glu

290 295 300

Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Gln Val Gln Lys Thr Arg

305 310 315 320

Arg Ile Thr Leu

<210> 22

<211> 325

<212> PRT

<213> 人工序列

<220>

<223> Galma_223690_mut1

<400> 22

Met Pro Ser Gln Phe Thr Ile Pro Asp Leu Leu Ile Thr Trp Pro Trp

1 5 10 15

Gln Glu Ile Thr Asn Pro Met Leu His Glu Val Asp Ala Glu Ala Asn

20 25 30

Glu Trp Val Gln Ser Leu Asn Leu Phe Glu Pro Lys Gln Phe Glu Lys

35 40 45

Phe Lys Ala Cys Asn Phe Asn Leu Leu Gly Ser Leu Val Gly Pro Leu

50 55 60

Pro Ser Arg Asp His Leu Arg Val Ser Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Met Ala Asn Lys Asp Glu Ala Met

85 90 95

Arg Ile Ala Arg Asp Val Met Gln Ala Phe Arg Asn Thr Asp Thr Pro

100 105 110

Ser Asn Ser Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Lys Arg Thr

115 120 125

Ile Glu Val Val Gly Glu Asp Leu Pro Gly Ile Glu Arg Phe Ile Ala

130 135 140

Asp Phe Asp Ala Tyr Thr Arg Ser Val Ile Gln Glu Ala Asp Asp Arg

145 150 155 160

Val Ala Gly His Ile Arg Asn Val Glu Asp Tyr Phe Ile Leu Arg Arg

165 170 175

Asp Thr Cys Gly Ala Lys Pro Ser Phe Ser Phe Tyr Gly Leu Gly Leu

180 185 190

Asn Ile Pro Thr Glu Val Phe Glu His Pro Leu Leu Ile Ser Met Val

195 200 205

Glu Ser Ala Thr Asp Leu Ile Ala Val Thr Asn Asp Met His Ser Tyr

210 215 220

Gly Leu Glu His Ser Arg Gly Leu Asp Gly His Asn Val Ile Thr Ala

225 230 235 240

Ile Met His Glu Tyr Gln Leu Asp Leu Gln Gly Ala Leu Tyr Trp Leu

245 250 255

Ser Gly Tyr Ala Thr Lys Thr Ile Ser Lys Phe Leu Thr Asp Arg Lys

260 265 270

Asn Leu Pro Ser Trp Gly Pro Thr Ile Asp Lys Ala Leu Glu Ile Tyr

275 280 285

Leu Asp Arg Leu Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser Tyr

290 295 300

Ser Thr Lys Arg Tyr Tyr Gly Lys Asn Gly Leu Lys Val Gln Lys Thr

305 310 315 320

Arg Arg Ile Thr Leu

325

<210> 23

<211> 326

<212> PRT

<213> 人工序列

<220>

<223> Hypsu_148385_mut1

<400> 23

Met Ser Ala Gln Tyr Thr Ile Pro Asp Leu Leu Ala Asn Trp Pro Trp

1 5 10 15

Gln Arg Val Thr Asn Ser Met Leu Asp Glu Val Arg Asp Glu Ala Asn

20 25 30

Glu Trp Val Met Ser Leu Gly Leu Phe Glu Pro Ala Gln Phe Lys Lys

35 40 45

Phe Lys Ala Cys Asp Phe Asn Leu Leu Ala Ser Phe Ile Gly Pro Leu

50 55 60

Glu Ser Lys Glu His Leu Arg Val Ala Cys Asp Leu Met Asn Phe Tyr

65 70 75 80

Phe Ala Phe Asp Glu Tyr Thr Asp Val Ala Asn Arg Glu Glu Ala Lys

85 90 95

Lys Ile Ser Gln Gly Val Met His Ala Phe Lys Thr Arg Ser Ala Glu

100 105 110

Pro Ser Ser Ser Lys Ile Thr Glu Met Ala Arg Gln Phe Phe Arg Arg

115 120 125

Thr Val Asp Val Val Gly Glu Asp Ser Pro Ala Ile Asn Gln Phe Ile

130 135 140

Thr Asp Phe Asp Thr Tyr Thr Thr Ala Val Ile Gln Glu Ala Asp Asp

145 150 155 160

Arg Thr Glu Gly Thr Ile Arg Asn Val Glu Asp Tyr Phe Thr Leu Arg

165 170 175

Arg Asp Thr Cys Gly Ala Lys Pro Ser Phe Ser Phe Phe Ala Leu Gly

180 185 190

Leu Asn Met Pro Thr Glu Val Phe Glu His Pro Leu Ile Met Ser Leu

195 200 205

Val Glu Arg Ala Thr Asp Leu Ile Ala Ile Val Asn Asp Met His Ser

210 215 220

Tyr Gly Leu Glu Arg Ala Arg Gly Leu Asp Gly His Asn Val Val Thr

225 230 235 240

Ser Ile Met Tyr Glu His Gln Leu Asp Leu Gln Gly Ala Leu His Trp

245 250 255

Leu Ala Gly Tyr Ala Glu Asp Thr Ile Ala Lys Phe Leu Ser Glu Lys

260 265 270

Glu Arg Leu Pro Ser Trp Gly Pro Ala Val Asp Val Ser Val Gln Glu

275 280 285

Phe Val Asp Arg Leu Gly Arg Cys Val Arg Gly Tyr Asp Ala Trp Ser

290 295 300

Tyr Glu Thr Asn Arg Tyr Tyr Gly Ser His Gly Leu Gln Ile Arg Gln

305 310 315 320

Thr Arg Gln Ile Thr Leu

325

<210> 24

<211> 620

<212> PRT

<213> 人工序列

<220>

<223> Ec.dxs_SL3

<400> 24

Met Ser Phe Asp Ile Ala Lys Tyr Pro Thr Leu Ala Leu Val Asp Ser

1 5 10 15

Thr Gln Glu Leu Arg Leu Leu Pro Lys Glu Ser Leu Pro Lys Leu Cys

20 25 30

Asp Glu Leu Arg Arg Tyr Leu Leu Asp Ser Val Ser Arg Ser Ser Gly

35 40 45

His Phe Ala Ser Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His

50 55 60

Tyr Val Tyr Asn Thr Pro Phe Asp Gln Leu Ile Trp Asp Val Gly His

65 70 75 80

Gln Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Arg Asp Lys Ile Gly

85 90 95

Thr Ile Arg Gln Lys Gly Gly Leu Thr Pro Phe Pro Trp Arg Gly Glu

100 105 110

Ser Glu Tyr Asp Val Leu Ser Val Gly His Ser Ser Thr Ser Ile Ser

115 120 125

Ala Gly Ile Gly Ile Ala Val Ala Ala Glu Lys Glu Gly Lys Asn Arg

130 135 140

Arg Thr Val Cys Val Ile Gly Asp Gly Ala Ile Thr Ala Gly Met Ala

145 150 155 160

Phe Glu Ala Met Asn His Ala Gly Asp Ile Arg Pro Asp Met Leu Val

165 170 175

Ile Leu Asn Asp Asn Glu Met Ser Ile Ser Glu Asn Val Gly Ala Leu

180 185 190

Asn Asn His Leu Ala Gln Leu Leu Ser Gly Lys Leu Tyr Ser Ser Leu

195 200 205

Arg Glu Gly Gly Lys Lys Val Phe Ser Gly Val Pro Pro Ile Lys Glu

210 215 220

Leu Leu Lys Arg Thr Glu Glu His Ile Lys Gly Met Val Val Pro Gly

225 230 235 240

Thr Leu Phe Glu Glu Leu Gly Phe Asn Tyr Ile Gly Pro Val Asp Gly

245 250 255

His Asp Val Leu Gly Leu Ile Thr Thr Leu Lys Asn Met Arg Asp Leu

260 265 270

Lys Gly Pro Gln Phe Leu His Ile Met Thr Lys Lys Gly Arg Gly Tyr

275 280 285

Glu Pro Ala Glu Lys Asp Pro Ile Thr Phe His Ala Val Pro Lys Phe

290 295 300

Asp Pro Ser Ser Gly Cys Leu Pro Lys Ser Ser Gly Gly Leu Pro Ser

305 310 315 320

Tyr Ser Lys Ile Phe Gly Asp Trp Leu Cys Glu Thr Ala Ala Lys Asp

325 330 335

Asn Lys Leu Met Ala Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Met

340 345 350

Val Glu Phe Ser Arg Lys Phe Pro Asp Arg Tyr Phe Asp Val Ala Ile

355 360 365

Ala Glu Gln His Ala Val Thr Phe Ala Ala Gly Leu Ala Ile Gly Gly

370 375 380

Tyr Lys Pro Ile Val Ala Ile Tyr Ser Thr Phe Leu Gln Arg Ala Tyr

385 390 395 400

Asp Gln Val Leu His Asp Val Ala Ile Gln Lys Leu Pro Val Leu Phe

405 410 415

Ala Ile Asp Arg Ala Gly Ile Val Gly Ala Asp Gly Gln Thr His Gln

420 425 430

Gly Ala Phe Asp Leu Ser Tyr Leu Arg Cys Ile Pro Glu Met Val Ile

435 440 445

Met Thr Pro Ser Asp Glu Asn Glu Cys Arg Gln Met Leu Tyr Thr Gly

450 455 460

Tyr His Tyr Asn Asp Gly Pro Ser Ala Val Arg Tyr Pro Arg Gly Asn

465 470 475 480

Ala Val Gly Val Glu Leu Thr Pro Leu Glu Lys Leu Pro Ile Gly Lys

485 490 495

Gly Ile Val Lys Arg Arg Gly Glu Lys Leu Ala Ile Leu Asn Phe Gly

500 505 510

Thr Leu Met Pro Glu Ala Ala Lys Val Ala Glu Ser Leu Asn Ala Thr

515 520 525

Leu Val Asp Met Arg Phe Val Lys Pro Leu Asp Glu Ala Leu Ile Leu

530 535 540

Glu Met Ala Ala Ser His Glu Ala Leu Val Thr Val Glu Glu Asn Ala

545 550 555 560

Ile Met Gly Gly Ala Gly Ser Gly Val Asn Glu Val Leu Met Ala His

565 570 575

Arg Lys Pro Val Pro Val Leu Asn Ile Gly Leu Pro Asp Phe Phe Ile

580 585 590

Pro Gln Gly Thr Gln Glu Glu Met Arg Ala Glu Leu Gly Leu Asp Ala

595 600 605

Ala Gly Met Glu Ala Lys Ile Lys Ala Trp Leu Ala

610 615 620

<210> 25

<211> 620

<212> PRT

<213> 人工序列

<220>

<223> Ec.dxs_SL5

<400> 25

Met Ser Phe Asp Ile Ala Lys Tyr Pro Thr Leu Ala Leu Val Asp Ser

1 5 10 15

Thr Gln Glu Leu Arg Leu Leu Pro Lys Glu Ser Leu Pro Lys Leu Cys

20 25 30

Asp Glu Leu Arg Arg Tyr Leu Leu Asp Ser Val Ser Arg Ser Ser Gly

35 40 45

His Phe Ala Ser Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His

50 55 60

Tyr Val Tyr Asn Thr Pro Phe Asp Gln Leu Ile Trp Asp Val Gly His

65 70 75 80

Gln Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Arg Asp Lys Ile Gly

85 90 95

Thr Ile Arg Gln Lys Gly Gly Leu Phe Pro Phe Pro Trp Arg Gly Glu

100 105 110

Ser Glu Tyr Asp Val Leu Ser Val Gly His Ser Ser Thr Ser Ile Ser

115 120 125

Ala Gly Ile Gly Ile Ala Val Ala Ala Glu Lys Glu Gly Lys Asn Arg

130 135 140

Arg Thr Val Cys Val Ile Gly Asp Gly Ala Ile Thr Ala Gly Met Ala

145 150 155 160

Phe Glu Ala Met Asn His Ala Gly Asp Ile Arg Pro Asp Met Leu Val

165 170 175

Ile Leu Asn Asp Asn Glu Met Ser Ile Ser Glu Asn Val Gly Ala Leu

180 185 190

Asn Asn His Leu Ala Gln Leu Leu Ser Gly Lys Leu Tyr Ser Ser Leu

195 200 205

Arg Asp Gly Gly Lys Lys Val Phe Ser Gly Val Pro Pro Ile Lys Glu

210 215 220

Leu Leu Lys Arg Thr Glu Glu His Ile Lys Gly Met Val Val Pro Gly

225 230 235 240

Thr Leu Phe Glu Glu Leu Gly Phe Asn Tyr Ile Gly Pro Val Asp Gly

245 250 255

His Asp Val Leu Gly Leu Ile Thr Thr Leu Lys Asn Met Arg Asp Leu

260 265 270

Lys Gly Pro Gln Phe Leu His Ile Met Thr Lys Lys Gly Arg Gly Tyr

275 280 285

Glu Pro Ala Glu Lys Asp Pro Ile Thr Phe His Ala Val Pro Lys Phe

290 295 300

Asp Pro Ser Ser Gly Cys Leu Pro Lys Ser Ser Gly Gly Leu Pro Ser

305 310 315 320

Tyr Ser Lys Ile Phe Gly Asp Trp Leu Cys Glu Thr Ala Ala Lys Asp

325 330 335

Asn Lys Leu Met Ala Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Met

340 345 350

Val Glu Phe Ser Arg Lys Phe Pro Asp Arg Tyr Phe Asp Val Ala Ile

355 360 365

Ala Glu Gln His Ala Val Thr Phe Ala Ala Gly Leu Ala Ile Gly Gly

370 375 380

Tyr Lys Pro Ile Val Ala Ile Tyr Ser Thr Phe Leu Gln Arg Ala Tyr

385 390 395 400

Asp Gln Val Leu His Asp Val Ala Ile Gln Lys Leu Pro Val Thr Phe

405 410 415

Ala Ile Asp Arg Ala Gly Ile Val Gly Ala Asp Gly Gln Thr His Gln

420 425 430

Gly Ala Phe Asp Leu Ser Tyr Leu Arg Cys Ile Pro Glu Met Val Ile

435 440 445

Met Thr Pro Ser Asp Glu Asn Glu Cys Arg Leu Met Leu Tyr Thr Gly

450 455 460

Tyr His Tyr Asn Asp Gly Pro Ser Ala Val Arg Tyr Pro Arg Gly Asn

465 470 475 480

Ala Val Gly Val Glu Leu Thr Pro Leu Glu Lys Leu Pro Ile Gly Lys

485 490 495

Gly Ile Val Lys Arg Arg Gly Glu Lys Leu Ala Ile Leu Asn Phe Gly

500 505 510

Thr Leu Met Pro Glu Ala Ala Lys Val Ala Glu Ser Leu Asn Ala Thr

515 520 525

Leu Val Asp Met Arg Phe Val Lys Pro Leu Asp Glu Ala Leu Ile Leu

530 535 540

Glu Met Ala Ala Ser His Glu Ala Leu Val Thr Val Glu Glu Asn Ala

545 550 555 560

Ile Met Gly Gly Ala Gly Ser Gly Val Asn Glu Val Leu Met Ala His

565 570 575

Arg Lys Pro Val Pro Val Leu Asn Ile Gly Leu Pro Asp Phe Phe Ile

580 585 590

Pro Gln Gly Thr Gln Glu Glu Met Arg Ala Glu Leu Gly Leu Asp Ala

595 600 605

Ala Gly Met Glu Ala Lys Ile Lys Ala Trp Leu Ala

610 615 620

<210> 26

<211> 659

<212> PRT

<213> 柱状田头菇

<400> 26

Met Pro Gly Ser Ala Asn Trp Thr Ala Asp Arg Phe Tyr Ile Pro Asp

1 5 10 15

Thr Leu Ala Asn Trp Pro Trp Pro Arg Ala Ile Asn Pro Ala Tyr Glu

20 25 30

Glu Cys Lys Ala Ala Ser Ala Ala Trp Cys Glu Lys Tyr Gly Ala Phe

35 40 45

Ser Ala Arg Ala Gln Lys Ala Phe Asn Leu Cys Asp Phe Asn Leu Leu

50 55 60

Ala Ser Leu Ala Tyr Ala Gly Leu Pro Ala Asp Val Asn Arg Val Gly

65 70 75 80

Cys Asp Leu Met Asn Leu Phe Phe Val Val Asp Glu His Thr Asp Ala

85 90 95

Met Asp Ala Arg Ser Val Gln Asp Trp Val Asp Ile Val Val Asp Ala

100 105 110

Leu His His Pro Arg Thr Pro Arg Pro Ala Gly Glu Pro Lys Val Gly

115 120 125

Glu Ile Ala Arg Thr Phe Trp Glu Asn Gly Ile Lys Cys Met Gly Pro

130 135 140

Thr Ala Gln Arg Arg Phe Val Glu Thr Phe Thr Thr Tyr Leu Gln Ser

145 150 155 160

Val Val Thr Gln Ala Gln Asp Arg Asp Lys His Leu Phe Arg Asp Val

165 170 175

Asp Ser Tyr Met Glu Val Arg Arg Asp Thr Ile Gly Ala Lys Pro Ser

180 185 190

Phe Ala Leu Leu Glu His Asp Met Glu Leu Pro Asp Asp Val Phe Tyr

195 200 205

His Pro Leu Leu Glu Lys Leu Arg Glu Trp Ala Ile Asp Met Leu Ile

210 215 220

Leu Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ser Arg Gly Asp

225 230 235 240

Asp Gly His Asn Ile Ile Arg Leu Ala Met Leu Gln Glu Asn Thr Asn

245 250 255

Val His Gly Ala Leu Arg Phe Val Ser Lys Met His Asp Asp Leu Ala

260 265 270

Glu Lys Phe Leu Ser Asn Tyr Gln Gly Met Pro Ser Phe Thr Pro Gln

275 280 285

Ile Asp Ala Trp Val Thr Arg Tyr Ile Asp Gly Leu Gly Asn Trp Val

290 295 300

Arg Ala Asn Asp Ser Trp Ser Phe Glu Ser Trp Arg Tyr Phe Lys Gly

305 310 315 320

Asp Val Leu Arg Val Gln Ala Glu Arg Trp Val Glu Leu Leu Pro Pro

325 330 335

Ala Pro Lys Asp Glu Leu Thr Ser Ser Ile Pro Pro Glu Ser Arg Trp

340 345 350

Ile Lys Pro Ala Val Glu Pro Ser Arg Ala Arg Pro Asn Asn Val Gly

355 360 365

Ile Val Ala Leu Asp Thr Tyr Thr Pro Thr Ser Glu Asp Asp Phe Gln

370 375 380

Thr Leu Ala Val Lys Thr Val Ser Ser Leu Leu Ser Lys Tyr Asn Ile

385 390 395 400

Asn Pro Val Ser Val Gly Arg Leu Asp Ile Cys Ile Glu Arg Ala Ala

405 410 415

Asp Pro Tyr Ile Ile Tyr Ala Leu Arg Asp Ala Phe Ala Ser Ala Gly

420 425 430

Asn Thr Asp Val Glu Ala Ile Val Ser Ser Ser Lys Ser Val Val Gly

435 440 445

Leu Phe Asn Ala Ile Asn Trp Val Glu Ser Ser Ser Trp Asp Gly Arg

450 455 460

Tyr Ala Ile Val Phe Ala Gly Asp Leu Ser Ser Gly Val Ser Ala Ala

465 470 475 480

Leu Val Gly Pro Asp Ala Pro Ile Val Val Glu Pro Thr Arg Gly Thr

485 490 495

Tyr Leu Gly Asp Pro Ile Ala Ser Thr Asp Glu Ala Gln Gly Ser Tyr

500 505 510

Ile Asp Ser Leu Phe Gln Ser Tyr Ser His Tyr Arg Lys Lys His Pro

515 520 525

Gln Phe Ser Lys Thr Ser Gly Ala Pro Asn Gly Ala His Thr Pro Thr

530 535 540

Thr Thr Asn Gly Ser Ile Lys Ser Asn Gly Phe Val Ser Gly Asp Thr

545 550 555 560

Asn Gly His Ala Asn Gly Asn Gly His Val Gln Thr Arg Ser Ser Thr

565 570 575

Pro Ser Ser Ser Ser Ser Ser Thr Ser Ser Pro Ser Phe Asp Tyr Met

580 585 590

Ile Leu His Asp Arg His Gly Lys Ile Pro Thr Gly Ala Gly Ser Ile

595 600 605

Tyr Leu Gly Leu Ala Ser Leu Ile Thr Asp Ile Ala Pro Glu Thr Leu

610 615 620

Ala Gly Lys Ser Ile Gly Val Phe Gly Phe Ala Asn Ser Thr Ser Thr

625 630 635 640

Phe Phe Gly Ile Arg Val Ala Gly Asp Cys Ser Val Ile Cys Lys Gln

645 650 655

Leu Gln Ala

<210> 27

<211> 353

<212> PRT

<213> 柱状田头菇

<400> 27

Met Ser Glu Gln Gln Tyr Thr Leu Pro Asp Leu Leu Gln Asn Trp Pro

1 5 10 15

Trp Asn Arg His Leu Ser Pro Tyr Tyr Glu Glu Ala Lys Arg Glu Ser

20 25 30

Ser Ala Trp Val Glu Ser Phe Lys Pro Phe Asp Gln Asp Gly Gln Arg

35 40 45

Ala Phe Asp Ala Tyr Leu Leu Ala Ser Leu Thr Tyr Ser His Gly Ser

50 55 60

Arg Glu Phe Val Arg Leu Gly Cys Asp Leu Met Asn Phe Tyr Phe Val

65 70 75 80

Tyr Asp Glu Tyr Thr Asp Val Ser Asp Ser Ala Val Ala Asp Arg Leu

85 90 95

Ala Asn Ile Val Ile Asp Ala Met Arg Asn Pro Glu Asn Ser Ser Gln

100 105 110

Ser Gly Asp His Leu Leu Gly Lys Met Thr Lys His Phe Trp Thr Arg

115 120 125

Ala Leu Ala Met Ala Pro Ala Gly Ser Pro Cys Phe Glu His Phe Ile

130 135 140

Thr Thr Ser Glu Thr Tyr Leu Arg Ala Val Thr Gln Glu Ala Glu Asp

145 150 155 160

Arg Ala Asn Lys Arg Val Arg Lys Val Asp Asp Tyr Leu Arg Leu Arg

165 170 175

Arg Asp Thr Cys Gly Ala Arg Pro Thr Leu Ala Leu Ile Glu Phe Gly

180 185 190

Leu Asn Leu Pro Asn Glu Val Val Arg His Pro Ser Leu Val Ala Leu

195 200 205

Thr Glu Ala Ala Val Asp Leu Ile Ile Leu Val Asn Asp Met His Ser

210 215 220

Tyr Val Arg Glu Leu Ser Cys Gly His Glu Asn His Asn Leu Ile Thr

225 230 235 240

Ala Ile Met Leu Glu His Arg Leu Asn Gln Gln Asp Ala Phe His Trp

245 250 255

Leu Gly Ser His Cys Ser Arg Val Val Asp Gln Phe Leu Ser Asp Leu

260 265 270

Asp Glu Leu Pro Ser Trp Gly Glu Pro Thr Asp Ser Gly Val Arg Asp

275 280 285

Tyr Ile Asn Gly Leu Gly Gln Trp Val Arg Gly Asn Asp Asp Trp Ser

290 295 300

Thr Glu Ser Lys Arg Tyr Tyr Gly Glu Asp Gly Glu Thr Ile Arg Gln

305 310 315 320

Glu Arg Leu Val Thr Thr Arg Ser Gly Glu Ser Asn Tyr Ile Lys Phe

325 330 335

Gly Gln Val Gly Val Gln Asp Ser Val Arg Ile Gln Pro Ile Glu Ala

340 345 350

Asn

<210> 28

<211> 346

<212> PRT

<213> 柱状田头菇

<400> 28

Met Cys Ala Ser Ala Thr Arg Pro Gln Pro Ser Ala Ser Asn Asn Val

1 5 10 15

Lys Lys Ile Ile Leu Pro Asp Leu Val Ser His Cys Thr Phe Lys Leu

20 25 30

Arg His Asn Arg His Arg Lys Gln Val Thr Thr Glu Thr Lys Lys Trp

35 40 45

Leu Phe Lys Asp Gly Asn Leu Leu Gly Gln Lys Glu Arg Ala Tyr His

50 55 60

Gly Leu Lys Cys Gly Leu Leu Thr Ser Met Cys Tyr Pro Asp Ala Gly

65 70 75 80

Tyr Pro Gln Leu Arg Val Val Asn Asp Phe Leu Thr Tyr Leu Phe His

85 90 95

Leu Asp Asn Leu Ser Asp Glu Met Asp Asn Arg Gly Thr Thr Thr Thr

100 105 110

Ala Asp Glu Val Leu Asn Ser Leu Tyr His Pro His Thr Trp Arg Ser

115 120 125

Ser Ala Arg Val Gly Lys Met Thr Arg Asp Phe Tyr Lys Arg Leu Val

130 135 140

Leu Thr Ala Ser Pro Gly Ala Gln Gln Arg Phe Ile Glu Thr Phe Asp

145 150 155 160

Phe Phe Phe Gln Ser Val Thr Gln Gln Ala Leu Asp Arg Ala Ser Gly

165 170 175

Val Ile Pro Asp Leu Glu Ser Tyr Ile Ser Leu Arg Arg Asp Thr Ser

180 185 190

Gly Cys Lys Pro Cys Trp Ala Met Ile Glu Tyr Ala Asn Asn Leu Asp

195 200 205

Ile Pro Asp Glu Val Met Asp His Pro Ile Ile Arg Ser Leu Gly Glu

210 215 220

Ala Thr Asn Asp Leu Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Ser

225 230 235 240

Val Glu Gln Ser Lys Gly His Thr His Asn Met Ile Pro Val Val Met

245 250 255

Tyr Gln Glu Gly Leu Asp Leu Gln Ala Ala Val Asp Phe Val Gly Asp

260 265 270

Met Cys Arg Gln Ser Ile Asn Arg Phe Val Glu Glu Lys Ala Arg Leu

275 280 285

Pro Ser Trp Gly Pro Lys Ile Asp Gln Asp Val Ala Ile Tyr Val Gln

290 295 300

Gly Leu Ala Asp Trp Ile Val Gly Ser Leu His Trp Ser Phe Glu Thr

305 310 315 320

Glu Arg Tyr Phe Gly Lys Ser Gly Arg Gln Val Lys Ala Ser Arg Ile

325 330 335

Val Asp Leu Leu Pro Arg Gln Arg Leu Pro

340 345

<210> 29

<211> 372

<212> PRT

<213> 柱状田头菇

<400> 29

Met Thr Ala Ala Pro Leu Thr Phe Thr Leu Pro Asp Leu Leu Ala Asn

1 5 10 15

Phe Pro Trp Lys Arg Asn Leu Ser Glu Tyr Tyr Pro Glu Cys Lys Thr

20 25 30

Glu Ser Ser Ala Trp Thr Glu Ser Phe His Pro Phe Asp Asp Glu Gly

35 40 45

Leu Lys Gly Phe Asn Leu Cys Asp Phe Asn Leu Leu Ala Ser Leu Ala

50 55 60

Tyr Ser Pro Arg Glu Arg Glu Ile Ile Arg Leu Gly Cys Asp Leu Met

65 70 75 80

Asn Ile Phe Tyr Val Phe Asp Glu Tyr Thr Asp Ile Ala Asp Gly Asp

85 90 95

Gly Ala Asp Lys Ile Arg Asp Ile Ile Met Asp Ala Phe Arg Asn Pro

100 105 110

His Lys Pro Arg Pro Glu Gly Glu Leu Leu Val Gly Glu Met Ala Arg

115 120 125

Asp Phe Trp Ile Arg Ala Ser Gly Tyr Val Ser Pro Asp Ala His Cys

130 135 140

Leu Thr His Phe Leu Arg Asp Phe Asp Thr Tyr Thr Ala Ala Val Val

145 150 155 160

Arg Glu Ala Asp Asp Arg Ala Lys Arg Val Tyr Arg Thr Phe Glu Asp

165 170 175

Tyr Leu Ser Ile Arg Arg Asp Ser Ser Gly Cys Leu Pro Ser Phe Ala

180 185 190

Leu Cys Glu Phe Gly Leu Asp Leu Pro Glu Glu Ala Tyr His His Pro

195 200 205

Arg Met Ala Ala Leu Arg Glu Gln Ser Thr Asp Leu Ile Ala Ile Gly

210 215 220

Asn Asp Ile Asp Ser Tyr Ala Met Glu Lys Ala Arg Gly Leu Glu Leu

225 230 235 240

His Asn Ser Val Glu Leu Ile Ile Asn Glu His Gly Leu Asp Val Gln

245 250 255

Gly Ala Ile Asn Trp Leu Glu Arg Tyr Ala Ala Gly Val His Ala Ser

260 265 270

Phe Leu Asp Asn Val Ala Asn Met Pro Ser Trp Gly Glu Asp Val Asp

275 280 285

Arg Arg Val Lys Met Tyr Ile Asp Gly Leu Ala Gln Trp Val Arg Gly

290 295 300

Asn Asp Asp Trp Thr Phe Glu Ser Gly Arg Tyr Phe Gly Asp Lys Gly

305 310 315 320

Leu Glu Val Gln Lys Thr Arg Val Met Ser Leu Leu Pro Ala Ser Lys

325 330 335

Val Ser Leu Arg Ser Arg Pro Lys Ala Val Gly His Val Pro Lys Lys

340 345 350

Leu Leu Arg Tyr Phe Arg Tyr Ser Thr Met Tyr Phe Phe Gly Phe His

355 360 365

Val Leu Ala Lys

370

<210> 30

<211> 342

<212> PRT

<213> 柱状田头菇

<400> 30

Met Ser Ala Leu Pro Ser Gln Phe Lys Leu Pro Asp Leu Leu Ser Thr

1 5 10 15

Cys Pro Leu Lys Asp Gly Thr Asn Pro Ala Tyr Lys Lys Ala Ala Ala

20 25 30

Glu Ser Arg Ala Trp Ile Gly Ser Tyr Asn Met Phe Ala Asp Arg Lys

35 40 45

Arg Ala Phe Phe Ile Gln Gly Gln Asn Glu Leu Leu Cys Ser His Val

50 55 60

Tyr Cys Tyr Ala Gly Tyr Glu Gln Leu Arg Thr Thr Cys Asp Phe Val

65 70 75 80

Asn Leu Leu Phe Val Val Asp Glu Val Ser Asp Glu Gln Ser Gly Glu

85 90 95

Asp Ala Arg Ala Thr Gly Gln Val Phe Val Asn Ala Met Lys Tyr Ala

100 105 110

Asp Trp His Asp Gly Ser Lys Leu Ala Lys Leu Thr Lys Asp Phe Arg

115 120 125

Val Arg Phe Leu Arg Leu Ala Gly Pro Lys Asn Val Ala Arg Phe Val

130 135 140

Ala Leu Cys Glu Ser Tyr Thr Ala Cys Val Gly Lys Glu Ala Glu Leu

145 150 155 160

Arg Glu Ser Gly Gln Val Leu Gly Val Lys Glu Phe Ile Pro Leu Arg

165 170 175

Arg Gln Asn Ser Ala Val Leu Leu Cys Phe Ser Leu Val Glu Tyr Ile

180 185 190

Leu Gly Ile Asp Leu Asp Asp Glu Val Tyr Arg Asp Glu Asn Phe Leu

195 200 205

Asn Ala Tyr Trp Ala Ala Cys Asp His Val Cys Trp Ala Asn Asp Val

210 215 220

Tyr Ser Tyr Asp Met Glu Gln Ser Lys Gly Leu Ser Asn Asn Asn Ile

225 230 235 240

Val Thr Val Leu Met Glu Glu Asn His Thr Ser Leu Gln Asp Thr Ser

245 250 255

Asp Tyr Ile Gly Glu Lys Cys Ala Glu Phe Val Gln Ile Tyr Leu Thr

260 265 270

Ser Lys Lys Arg Leu Ser Pro Ser Leu Gly Pro Asp Ala Ala Leu Phe

275 280 285

Leu Glu Ser Ile Gly Ser Trp Met Val Gly Asn Leu Ala Trp Ser Phe

290 295 300

Glu Thr Ser Arg Tyr Phe Gly Ser Arg His Leu Glu Val Lys Glu Thr

305 310 315 320

Gly Ile Val Ile Leu Arg Pro Arg Glu Leu Pro Glu Asp Gly Ser Ser

325 330 335

Ser Asp Ser Asp Glu Glu

340

<210> 31

<211> 387

<212> PRT

<213> 柱状田头菇

<400> 31

Met Ser Phe Phe Lys Ser Ser Gln Pro Thr Ile Tyr Ile Pro Asp Thr

1 5 10 15

Leu Arg Asn Trp Pro Trp Pro Arg Glu Ile Asn Pro His Tyr Glu Glu

20 25 30

Cys Lys Arg Glu Ser Ala Ala Trp Val Glu Lys Phe Gly Ala Phe Ser

35 40 45

Ala Lys Ala Gln Lys Ala Phe Asn Lys Cys Asp Phe Asn Leu Leu Ala

50 55 60

Ser Leu Ala Trp Ser Arg Val Asn Arg Asp Gly Cys Arg Ile Gly Cys

65 70 75 80

Asp Leu Met Asn Leu Phe Phe Val Phe Asp Glu Trp Ser Asp Val Ser

85 90 95

Asp Ala Glu Glu Thr Arg Arg Met Ala Asp Ile Ile Met Asp Ala Leu

100 105 110

Tyr Asp Pro His Lys Pro Arg Pro Thr Gly Glu Trp Val Gly Gly Glu

115 120 125

Val Thr Arg Gln Tyr Trp Leu Asn Ala Ile Arg Thr Ala Thr Pro Ser

130 135 140

Ala Gln Lys Arg Phe Ile Lys Ala Phe Lys Leu Tyr Thr Asp Ser Val

145 150 155 160

Val Gln Gln Ser Ala Asp Arg Asp Lys His Leu Ile Arg Asp Ile Asp

165 170 175

Ser Tyr Phe Glu Val Arg Arg Asp Thr Ile Gly Ala Lys Pro Ser Phe

180 185 190

Ala Ile Asn Glu Val His Met Asn Leu Pro Asp Tyr Val Met Glu His

195 200 205

Pro Val Ile Lys Asn Leu Thr Ala Tyr Cys Ile Asp Met Leu Cys Ile

210 215 220

Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ser Arg Gly Asp Asp

225 230 235 240

Gly His Asn Leu Val Thr Ile Val Met His Gln Leu Asn Leu Asp Val

245 250 255

Gln Gly Ala Phe Asp Trp Ile Gly Lys Leu His Asp Glu Leu Val Asp

260 265 270

Lys Phe Leu Glu Glu Tyr Lys Asn Val Pro Thr Phe Lys Asp Lys Gln

275 280 285

Val Thr Lys Glu Cys Ala Glu Tyr Ala Phe Gly Leu Gly Asn Trp Val

290 295 300

Arg Gly Asn Asp Gln Trp Ser Phe Glu Ser Glu Arg Tyr Phe Gly Lys

305 310 315 320

Asp Gly Met Arg Val Leu Thr Glu Arg Thr Val Val Leu Leu Pro Lys

325 330 335

Lys Arg Glu Pro Pro Pro Lys Pro Leu Asp Glu Asp Pro Ile Tyr Ser

340 345 350

Ala Leu Pro Trp Trp Gly Trp Thr Ala Leu Phe Gly Phe Leu Ala Thr

355 360 365

Ala Phe Thr Phe Ser Ala Arg Gln Leu Ser Thr Arg Ile Ser Ala Asn

370 375 380

Leu Ile Ala

385

<210> 32

<211> 430

<212> PRT

<213> 柱状田头菇

<400> 32

Met Ala Ser Ser Leu Leu Glu Pro Ser Leu Ala Ala Ile Ala Leu Val

1 5 10 15

Ile Leu Leu Ala Ser Val Ser Leu Ser Arg Lys Lys Arg Pro Ala Ala

20 25 30

Pro Glu Pro Gln Gly Leu Ser Val Leu Gly Asn Leu Phe Asp Ile Pro

35 40 45

Lys Arg Ala Ser Ser Ile Ile Tyr Leu Ala Leu Gly Lys Pro Tyr Asn

50 55 60

Thr Leu Thr Lys Arg Ala Val Ser Gln Leu Gln Gly Tyr Thr Pro Gly

65 70 75 80

Ser His Ile Asp Ala Thr Ser His Ser Pro Arg Val Phe Arg Leu Pro

85 90 95

Asn Leu Glu Glu Thr Phe Ser Val Phe Pro Asp His Gly Leu Asn Pro

100 105 110

Asn Tyr Thr Ser Ala Arg Thr Asp Ser Arg Ala Trp Ile Asn Gln Tyr

115 120 125

Thr Lys Val Val Cys Gly Pro Lys Met Val Ala Phe Met Asn Asn Cys

130 135 140

Glu Phe Glu Leu Ser Asn Ser His Cys Tyr Pro Tyr Ala Gly Tyr Lys

145 150 155 160

Gly Leu Lys Ala Thr Met Asp Leu Thr Asn Ile Leu Trp Leu Tyr Asp

165 170 175

Glu Tyr Thr Asp Thr Gly Ser Gly Ala Glu Ala Val Lys Ala Ala Gly

180 185 190

Ile Val Ala Arg Ala Leu Arg Glu Pro Asp Tyr Asp Asp Gly Thr Trp

195 200 205

Val Cys Arg Met Met Lys Ser Phe Lys Gln Asn His Ile Asp Lys Ala

210 215 220

Gly Pro Gly Val Ala Arg Arg Phe Ile Asp Asn Phe Cys Asn Tyr Val

225 230 235 240

Glu Val Val Gly Arg Glu Ala Glu Leu Arg Glu Lys Asn Glu Val Leu

245 250 255

Asp Ile Pro Asn Tyr Val Thr Phe Arg Arg Glu Thr Ser Ala Val Arg

260 265 270

Thr Cys Phe Asp Leu Val Glu Tyr Cys Leu Asp Leu Asp Leu Pro Gln

275 280 285

Tyr Val His Asp Asp Pro Val Phe Ile Ser Gly Tyr Asn Ala Gly Met

290 295 300

Asp Leu Val Phe Trp Ala Asn Asp Leu Val Ser Tyr Asn Met Glu Gln

305 310 315 320

Ser Lys Gly His Ser Gly Ala Asn Val Val Thr Val Ile Met Lys Ser

325 330 335

Lys Gly Val Asp Leu Gln Thr Ala Val Asp Phe Leu Gly Gly Tyr Cys

340 345 350

Glu Ala Leu Thr Ala Gln Leu Leu Glu Ala Lys Arg Ile Leu Gln Ala

355 360 365

Arg Ser Asp Ala Ala Tyr Ser Arg Asp Val Val Arg Leu Met Asp Ala

370 375 380

Phe Gly Asp Trp Val Arg Gly Asn Val Ala Trp Ser Phe Glu Thr Glu

385 390 395 400

Arg Tyr Phe Gly Lys Glu Asn Lys Arg Val Lys Glu Thr Leu Leu Val

405 410 415

Glu Leu Lys Glu Pro Phe Val Gly Ala Leu Ala Leu Lys Glu

420 425 430

<210> 33

<211> 358

<212> PRT

<213> 柱状田头菇

<400> 33

Met Asn Ala Ser Pro Phe Leu Asn Glu Ser Ser Pro Thr Arg Pro Thr

1 5 10 15

Ser Phe Val Leu Pro Asp Leu Val Ser His Cys Lys Phe Pro Leu Ser

20 25 30

Tyr His Pro Asn Gly Asp Glu Ile Ala Gln Glu Ser Val Asp Trp Leu

35 40 45

Asp Ser Ser Cys Pro Asp Leu Thr Ala Lys Gln Arg Arg Ala Leu Arg

50 55 60

Val Leu Gln Ser Gly Glu Leu Thr Ala Tyr Cys Tyr Asn Gln Ala Thr

65 70 75 80

Ser Pro Glu Arg Leu Arg Val Val Ser Asp Phe Leu Thr Tyr Leu Phe

85 90 95

His Leu Asp Asn Ile Ser Asp Gly Met Met Thr Arg Glu Thr Asp Val

100 105 110

Leu Ala Asp Val Val Met Asn Ala Phe Trp Phe Thr Asp Lys Tyr Met

115 120 125

Pro Thr Arg Gly Pro Gly Lys Glu Gln Leu Asp Glu Glu Leu Asn Pro

130 135 140

Gly Lys Leu Ala Arg Asp Phe Trp Ser Arg Ala Ile Ala Asp Cys Gly

145 150 155 160

Val Gly Val Gln Ala Arg Phe Lys Glu Thr Met Gly Leu Phe Phe Glu

165 170 175

Ala Val Asn Ile Gln Ala Arg Met Arg Asp Glu Asp Thr Ile Pro Asp

180 185 190

Leu Glu Ser Tyr Ile Asp Val Arg Arg Asp Thr Ser Gly Cys Lys Pro

195 200 205

Ser Trp Val Leu Ile Glu Tyr Ala Leu Gly Ile Asp Leu Pro Asp His

210 215 220

Val Val Asp His Pro Ile Met Gln Ala Leu Asn Gln Gly Thr Asn Asp

225 230 235 240

Leu Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Asn Val Glu Gln Ser

245 250 255

Arg Gly Asp Thr His Asn Met Ile Val Ile Leu Met Glu Tyr His Gly

260 265 270

His Thr Leu Gln Ser Ala Val Asp Tyr Val Gly Glu Leu Cys Ala Gln

275 280 285

Thr Ile Asp Thr Phe Cys Glu Asn Lys Glu Arg Leu Pro Ser Trp Gly

290 295 300

Pro Glu Ile Asp Asp Met Val Ala Arg Tyr Val Lys Gly Leu Gln Asp

305 310 315 320

Trp Ile Val Gly Ser Leu His Trp Ser Phe Gln Thr Gln Arg Tyr Phe

325 330 335

Gly Lys Asp Gly Leu Asp Ile Lys Lys His Arg Phe Val Lys Leu Leu

340 345 350

Pro Leu Glu Ala Ala Lys

355

<210> 34

<211> 389

<212> PRT

<213> 柱状田头菇

<400> 34

Met Val Trp Asp Phe Val Leu Ser Leu Phe His Ser Leu Leu Ala Ala

1 5 10 15

Phe Gln Thr Leu Thr Ser Trp Leu Thr Gly Ser Phe Leu Phe Asn Asn

20 25 30

Lys Met Ala Pro Ala Pro Asn Pro Ala Pro Val Thr Phe Ile Leu Pro

35 40 45

Asp Leu Glu Lys Thr Phe Asn Ser Leu Pro Asp Asp Gly Leu Asn Pro

50 55 60

His His Asp Val Ala Cys Ala Glu Ser Arg Glu Trp Phe Ala Lys Tyr

65 70 75 80

Asn Lys Lys Val Leu Gly Ala Gln Met Gln Glu Phe Phe Arg Arg Cys

85 90 95

Lys Phe Glu Leu Ile Thr Ser Tyr Thr Tyr Pro Tyr Val Asp Lys Glu

100 105 110

Gly Leu Arg Ala Thr Met Asp Trp His Asn Ile Leu Trp Phe Phe Asp

115 120 125

Glu Val Thr Asp Thr Glu Thr Gly Lys Asp Ala His Lys Ser Ala Ile

130 135 140

Ile Thr Ile Arg Thr Leu Arg Glu Pro Asp Phe Asp Asp Gly Ser Ser

145 150 155 160

Leu Cys Arg Met Val Arg Asp Phe Arg Leu Ser His Leu Ser Arg Ala

165 170 175

Gly Pro Glu Cys Thr Arg Arg Phe Leu Glu His Cys Asp Val Ala Phe

180 185 190

His Ala Gly Ala Val Glu Ala Glu Leu Arg Glu Lys Gly Glu Val Leu

195 200 205

Ser Ile Glu Gly Tyr Leu Lys Leu Arg Arg Glu Thr Ser Gly Ala Arg

210 215 220

Thr Cys Phe Asp Met Ala Glu Tyr Leu Met Asp Ile Asp Leu Pro Gln

225 230 235 240

Asp Met Tyr Asp Asp Pro Val Phe Gln Lys Gly Tyr Ile Ala Ala Leu

245 250 255

Asp Leu Ile Phe Leu Ala Asn Asp Leu Tyr Ser Tyr Asn Met Glu Gln

260 265 270

Ala Lys Gly His Asn Gly Ala Asn Val Leu Thr Val Val Met Lys Glu

275 280 285

Thr Lys Leu Asn Leu Gln Ser Ala Ala Asp Tyr Val Gly Val Leu Cys

290 295 300

Glu Lys Leu Ile Lys Gln Phe Gln Glu Ala Lys Ser Thr Leu Glu Asn

305 310 315 320

Arg Leu Ala Lys Glu Lys Asn Pro Ala Lys Ala Ala Ala Leu Lys Asp

325 330 335

Ala Ile Arg Ser Leu Val Gly Tyr Gly His Trp Val Arg Gly Asn Val

340 345 350

Glu Trp Ser Phe Glu Thr Glu Arg Tyr Phe Gly Lys Lys Asn Lys Glu

355 360 365

Ile Lys Lys Ser Arg Val Val Thr Leu Thr Pro Thr Asn Ser Val Asn

370 375 380

Arg Ala Leu Lys Ala

385

<210> 35

<211> 1173

<212> DNA

<213> 柱状田头菇

<400> 35

atgcatcacc atcaccatca ccacagctcg acccagctgt tcactcactc tccgaccaaa 60

atgcagatca tcctgccgga tctgctgtgc tcctggggat ataaagggtt tctcaaccct 120

cactatgagg gcgctaaggc ggaatccaac acctggattc gcccgctcgt agtgaaactt 180

ttcgacgaac gtggccagaa agcgttcgcc aaagattaca ccggtctgct ggcgtctatg 240

acttacccgc accacaacaa agaattcctg tgtgtggctt gcgatatgat gaacctgttc 300

tttgtttatg acgagtatac cgatattacc ccgccggaaa ccgcgcagcg tctggctaaa 360

atcgttgtaa atgctatgcg caatccggat gagatcgccg ctctgggtga agacagcatc 420

ggtactatga ccaagcagtt ctggcgccgc gctatgactc tgctgccacc gaacggttgt 480

aactctgaat cttgtatcca gcacttcatt gactacaccg aagaatatct gactgcagtg 540

acgcgtgaag cttgtgatcg tagcagcggc tctgtccacg ctgtaaaaga ctatctggcg 600

atgcgtcgtg caacctctgg cgcgggtctg atggttggtt tactggaatt tggcctcgat 660

ctgccggaag aagtcatgaa acatgaagtg atccaggaac tgtctactgg cgcgatcgac 720

atgtactgtc tgctgaacga catgcatagc tacgcatctg aactgtcgtc tggtcaggcc 780

tctcacaacg ttattaccgt cgtgatgcac gaacgtaacc tgtccctgca agaagctttt 840

gattggctgg cctcttacgc ggcaggcgta gtgaagggtt tcaaaaccaa cctgaaccgc 900

gttccttctt ttagcgaact ggaggaccac tctctgcacg gcgaaggcat gctccgtgat 960

cgcattcagc gttacatcga cggcctgggt caggcggttc gcgcggaaga tgattgggct 1020

tttgaaacta cccgctatca cggtcagaac ggcccgcaga ttaaactgac ccgtatgctg 1080

accatccgtc cgcaggttaa ggacaactac gctaaacagg ctccgcgcgt cgaggctcct 1140

gaacgcccga tgttactgga agtgcgtagt taa 1173

<210> 36

<211> 1050

<212> DNA

<213> 平田头菇

<400> 36

atgcatcacc atcaccatca ctcccaattt attatcccgg atctgttatc cacttggcct 60

tggcagcgcg tgtctaaccc gatgtggcgt gagattgatg aagaagcaaa cgcgtgggtg 120

cagtctttcg acctgtttga accgtcccag ttcgagaaat tcaagatgtg tgatttcaac 180

ctgctgggct cgatgattgg taccgtggaa accaaagatc atctgcgcat cagttgcgac 240

ctgatgaact tctatttcgc attcgacgaa tacacggaca tggcatccaa agacgaagcg 300

cgtaagatcg cccgtgacgt gatggatgca tttcgcaaca ccgaaaaacc agctcacaac 360

caaatcaccg aaatggcccg ccagtttttt aaacgtacga tcgacactgt aggtaaagac 420

ttgccgggcg tggaacgttt tatcgctgac ttcgatgctt atacgcattc cgtgatccag 480

gaggccgacg accgtgctac tggccacatc cgcagcgtca acgactattt cacgttacgc 540

cgcgacactt gcggcgcgaa accaagcttc agcttctttg gcctgggtct gaacattccg 600

gatgaggttt tccaccatcc tgtggttatt tccatgatcg aaggcgccac cgatctgatt 660

gctgttacga atgacatgca ctcatataac ctcgaacact cccgtggtct ggatggacac 720

aacgtaatca ctgctatcat gcacgaatac cagctggacc tgcagggcgc actgtactgg 780

ctatccggtt acgctaccca tacgattgct aacttcctgt cgaaccgccg taacctgccg 840

tcctggggcc cggctgtcga taaagcggta gaagaatttt tcgaccgtgt tggtcgttgt 900

gttcgtggct acgacgcttg gagctacgaa accaaacgct attacgggaa aaacggcctg 960

caggtgcaga agacccgtcg tatcaccctg cgcccgcgtg acgcgtctta cattactaag 1020

gagcagctgc aggtatctat caaagcataa 1050

<210> 37

<211> 1053

<212> DNA

<213> 平田头菇

<400> 37

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgttc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 38

<211> 1050

<212> DNA

<213> 纹缘盔孢伞

<400> 38

atgcatcacc atcaccatca cccgagccag ttcaccatcc ctgacctgct gatcacttgg 60

ccatggcagg aaatcaccaa cccgatgctg cacgaagtag acgctgaagc caatgaatgg 120

gttcagtcac tgaacctgtt cgaaccgaaa cagttcgaaa agttcaaagc ctgtaacttc 180

aacctgctgg gctccctggt tggaccgctg ccaagccgtg accaccttcg tgtgtcctgc 240

gacctgatga acttctactt cgctttcgat gagtacacgg acatggcaaa caaagatgaa 300

gctatgcgta tcgcccgtga cgtaatgcaa gcattccgta acactgacac gcctagcaac 360

tctaaaatca ctgaaatggc acgtcaattc ttcaaacgta ccatcgaggt tgtaggcgaa 420

gacctgccgg gcatcgaacg tttcatcgcc gatttcgatg catacactcg ttccgtgatt 480

caggaagcag atgatcgtgt tgcaggtcac attcgtaacg tggaggacta cttcattctg 540

cgccgtgata cgtgcggtgc taaaccgagc ttctctttct acggtctggg tctgaacatc 600

ccgaccgagg tctttgaaca cccgctgctg atttctatgg ttgaaagcgc aactgacctg 660

attgctgtca ccaatgacat gcactcttac ggtctggagc attctcgtgg cctggacggt 720

cacaatgtta tcaccgccat tatgcacgaa tatcagctgg acctacaggg tgccctgtac 780

tggctgtccg gctacgcaac taaaaccatc tccaaattcc tgactgatcg taaaaacctg 840

ccgtcctggg gcccaaccat tgacaaagct ctcgaaatct acctggaccg tttaggtcgt 900

tgcgtgcgtg gctacgatgc ttggtcctac agcactaagc gttactatgg taaaaacggt 960

ctgaaggttc aaaaaacccg ccgtatcact ttgaaaccgc gtgatgcagc ctacatcacg 1020

aaggatcagc tgcaggtgtc tatcgcttaa 1050

<210> 39

<211> 1053

<212> DNA

<213> 砖红韧黑伞

<400> 39

atgcatcacc atcaccatca ctccgctcag tacaccatcc ctgacttact ggcgaactgg 60

ccgtggcagc gcgtgaccaa cagcatgctg gacgaggtgc gcgacgaagc aaacgagtgg 120

gtgatgtccc tgggcctgtt tgagccggct cagtttaaaa aattcaaagc atgcgacttc 180

aaccttctgg caagctttat tggtccgctg gaaagtaaag aacaccttcg tgttgcttgc 240

gatctgatga atttctattt cgctttcgat gagtacactg acgttgctaa ccgtgaagag 300

gctaaaaaaa tctctcaggg cgttatgcac gcgtttaaaa ctcgttctgc agagccgtcc 360

agctccaaaa ttacggaaat ggcgcgtcag ttctttcgtc gtaccgttga cgtcgtcggt 420

gaagattccc cggccatcaa ccagttcatt accgacttcg acacctacac cacggctgtg 480

atccaggaag cagacgatcg caccgaaggt accatccgta atgttgagga ctatttcacc 540

ctccgtcgcg acacctgcgg cgccaaacct tctttttcgt tcttcgccct gggcctgaac 600

atgccgaccg aagtcttcga acaccctctg atcatgtctc tggtagagcg tgcaaccgac 660

ctgatcgcga ttgtgaacga catgcactcc tacggtctgg aacgtgctcg tggcctggat 720

ggtcataacg tagtaacgtc catcatgtac gagcatcagc tggacctgca gggtgcgctg 780

cactggctgg ccggttatgc cgaagacaca atcgccaagt tcctgtcgga aaaagaacgt 840

ctcccgtcct ggggtccggc ggtagatgta tccgtgcagg aatttgttga ccgtctgggc 900

cgttgcgtgc gtggttacga cgcttggtcc tatgaaacga accgctatta cggcagccac 960

ggtctacaga ttcgtcagac tcgtcaaatc accctgccat ccaacgactc tggctacatc 1020

actcgcaagc agatgggcgt ttctatcgct taa 1053

<210> 40

<211> 1980

<212> DNA

<213> 柱状田头菇

<400> 40

atgcctggat cagcgaactg gactgcggac cgattttaca ttcctgacac cctggctaat 60

tggccttggc cgagagccat caatcccgcg tacgaggagt gcaaggcggc ctccgctgcc 120

tggtgcgaga aatatggcgc tttctctgct cgcgcccaga aggccttcaa cctctgcgac 180

ttcaatttat tggcctcttt ggcgtatgct ggcctccctg cagacgtcaa tcgagtagga 240

tgtgacctca tgaacctatt ttttgtcgtc gacgagcaca cagacgctat ggatgctcgg 300

tccgttcagg actgggtaga catcgtcgtg gatgccctcc atcatcctca cacgccgaga 360

cctgcgggtg aaccgaaggt cggggagata gcccgcacct tctgggaaaa tggcatcaaa 420

tgcatgggac ccaccgccca acgccgattt gttgaaacat tcaccaccta ccttcagagt 480

gtcgtgactc aagcccaaga tcgcgacaag catctcttcc gcgatgtgga cagctacatg 540

gaggttcggc gagacaccat cggagccaag ccctcgttcg ccttgcttga gcacgacatg 600

gagcttccgg acgatgtctt ctatcaccct ctcctggaga agttgaggga gtgggcgatc 660

gacatgctga tccttggaaa tgacttgtgc tcctacaatg tcgagcaatc acgaggcgac 720

gacggccaca acatcatccg actcgcaatg cttcaagaaa acaccaacgt ccacggcgct 780

ctccgctttg tctcaaagat gcacgacgac ctcgccgaga agttcctttc caactaccaa 840

ggcatgccgt ctttcactcc acaaatcgac gcatgggtca ccaggtacat cgacggcctc 900

ggtaactggg ttcgcgcaaa cgattcatgg agcttcgaga gttggaggta tttcaaggga 960

gacgtgttgc gtgtccaggc agagcgttgg gtcgaattgc tccctccagc cccaaaggat 1020

gagctaacgt catcaatccc gcctgaatcc cggtggatca aacctgcggt cgagccttcg 1080

cgtgcgaggc caaataatgt cggtatcgtg gcactggata cctacacacc gacttcagag 1140

gatgatttcc agaccttggc tgtcaaaact gtgtcttcgc tcctctcgaa gtacaacatc 1200

aaccccgtct ctgtcggccg gctcgacatc tgtattgaga gggctgcaga tccttacata 1260

atttatgctc tgcgagacgc cttcgcctcc gctggcaaca cggacgtcga ggcgattgta 1320

agctcctcca agtctgtcgt cggattgttc aacgcaataa attgggtcga gtcatcgagc 1380

tgggacggcc gttacgccat cgtattcgcg ggagatctct cttcgggagt atctgcagct 1440

ttggttggcc cagacgcgcc catagtcgtg gaacctactc gaggaaccta tctcggcgat 1500

cccatcgcat caacagacga ggcacagggg tcatacattg attctctctt ccaatcatac 1560

tcgcattatc gcaaaaagca cccccagttc tccaagacct ctggtgctcc caacggagcg 1620

cacactccca caacaacgaa cggcagcatt aaatccaacg gattcgtcag tggagacacc 1680

aacggccacg cgaatgggaa tggccacgtt cagacccgtt cgtccacccc ctcttcttct 1740

tcttcctcca cgtcttcccc atctttcgac tacatgatcc tccacgaccg ccatggcaag 1800

attcccaccg gcgctgggtc catctatctc gggctggcct cgcttattac ggatatcgca 1860

ccggagacgc tggcgggcaa gagcatcggt gtatttgggt ttgcgaacag caccagcacc 1920

ttcttcggta ttcgcgttgc gggtgactgc agtgttatct gcaagcagct tcaggcctaa 1980

<210> 41

<211> 1062

<212> DNA

<213> 柱状田头菇

<400> 41

atgtccgaac agcagtacac tcttcccgac ctccttcaaa attggccatg gaaccgtcat 60

ctcagccctt attacgaaga ggcgaagagg gagtcgagtg catgggtcga atcattcaag 120

ccatttgatc aggatgggca gagggccttc gacgcttatc tcctcgccag cctgacctat 180

tcacatggga gcagggaatt tgtgcgtctc ggatgcgatc tcatgaactt ctacttcgtc 240

tacgatgaat acaccgacgt ttccgactcc gcggtggcgg acaggctggc caacattgtg 300

atcgatgcca tgagaaaccc cgaaaattca tctcaaagtg gagatcacct cctagggaag 360

atgactaaac atttctggac tcgagcgcta gcgatggcac cggcaggatc accctgtttc 420

gagcatttca tcaccacatc ggagacatac ctacgcgcag taacccaaga agcggaagac 480

agagcgaaca agcgcgtgcg taaggtggac gactacctcc gacttcgccg ggatacgtgt 540

ggcgcacgtc cgacgctcgc actgatcgag tttgggctca atttgcctaa tgaggtagtt 600

cgccatcctt ctttggtagc ccttacggaa gcggctgtgg atttgatcat cctcgttaac 660

gatatgcact cgtacgtgcg ggaattgtct tgtggacacg aaaaccacaa cctcatcact 720

gctattatgc ttgagcatcg tctcaatcgg caagacgcct tccattggct gggatcacac 780

tgcagcaggg ttgtggatca atttctctct gacttggacg agctaccgtc ctggggagag 840

cctaccgatt ctggagtgag ggattacatc aacggcctgg gacaatgggt tcgtggaaac 900

gacgattgga gtaccgagag caaaagatat tacggagaag atggggaaac aatacgacag 960

gagcgcttgg tcacaactcg ctccggggag tcgaattaca tcaaattcgg gcaagttggc 1020

gtacaagact cggtgcgcat ccaaccaatc gaagccaact ga 1062

<210> 42

<211> 1041

<212> DNA

<213> 柱状田头菇

<400> 42

atgtgtgcct ccgccacgcg cccccagcca tcggcgtcca acaacgtgaa gaagattatc 60

ctgcccgacc tcgtctcaca ttgcaccttt aagcttcgtc ataatcgcca caggaaacaa 120

gttaccaccg agacgaagaa gtggctgttc aaggacggca atctcctagg ccagaaggaa 180

agggcgtacc acggactcaa atgtggcctc ctcacatcta tgtgctaccc ggatgcaggg 240

tacccccaac tgcgcgtcgt caacgacttc ctcacctatc tcttccacct cgataactta 300

tcagacgaga tggacaatag gggcacaacc acgaccgctg atgaggttct aaactctctc 360

taccaccctc acacttggcg ttcgtcggcg agagttggga agatgacgcg ggatttctat 420

aagcggctcg ttcttacagc gtcgcctggg gctcagcagc gtttcattga gacgtttgat 480

ttcttcttcc agagtgtaac tcaacaagct ttggatcgtg cgagtggagt aatacccgac 540

ctcgagtctt acatctccct gcgtcgcgat acctcaggct gcaagccctg ctgggcgatg 600

atcgaatatg caaacaatct cgatatccct gatgaagtca tggatcatcc cattattcgc 660

agtctcggag aagctacgaa cgatttggtg acatggtcta acgacatctt ttcctacagt 720

gtagaacagt cgaaggggca tacacataat atgatccccg tggtcatgta ccaagaaggc 780

ttggaccttc aagctgccgt ggacttcgtc ggcgatatgt gcaggcagtc gatcaatcgc 840

tttgttgagg aaaaggcgcg tcttccttca tggggcccta agatcgacca ggacgtggcc 900

atctacgtcc aaggtctcgc cgactggatc gtcggttctc tccactggtc cttcgaaaca 960

gaacgctact ttggcaaatc aggccgccag gtgaaggcct cacggatagt tgacctcctt 1020

cccagacaac ggttacctta a 1041

<210> 43

<211> 1119

<212> DNA

<213> 柱状田头菇

<400> 43

atgaccgctg caccactcac tttcactctt cctgaccttc tggcgaattt tccttggaag 60

cgcaacttaa gcgaatacta tcccgaatgc aagacagagt ccagcgcatg gactgagtcc 120

tttcacccgt tcgacgacga gggtctcaaa gggttcaatc tctgtgattt caatctgctc 180

gcttctttgg catattcgcc ccgtgagcga gagatcatca ggctcgggtg cgatctcatg 240

aacatcttct acgtcttcga cgagtacacc gacatcgcgg acggtgacgg tgccgacaaa 300

attcgagaca tcatcatgga tgctttccgt aatcctcaca agcctaggcc cgaaggagag 360

ctgctagtcg gcgaaatggc acgcgacttc tggattcggg cgtccggcta cgtgtccccc 420

gacgcccact gcctgaccca ctttctccgt gatttcgaca cctacacggc tgcggtcgtg 480

cgggaagccg acgaccgcgc caaacgcgtc tatcgcacct tcgaagatta tctctccatc 540

cgccgggatt cctcaggctg cctgcccagc ttcgcgctct gtgagttcgg gcttgacctg 600

cccgaagagg catatcacca cccgcgaatg gctgcccttc gcgaacaaag cactgatcta 660

atcgctatcg gaaacgacat tgattcgtac gccatggaga aggcacgcgg gctggaactc 720

cataactccg ttgagctcat cataaatgag cacggccttg atgtccaagg cgctatcaac 780

tggctcgaac gttatgccgc aggggtccac gcatcgttcc tcgacaacgt cgcgaacatg 840

ccttcgtggg gcgaggacgt tgatcgacgt gtcaagatgt acattgatgg cctcgcgcaa 900

tgggtccgtg gtaatgatga ttggactttt gagagcgggc gctattttgg cgacaaaggc 960

ctggaagtcc agaagacgag agtcatgtcc ctcctgcccg ctagcaaggt ttcgttaaga 1020

agtcggccta aagccgtcgg acacgtaccc aagaaactct taagatattt ccgatactct 1080

acgatgtatt tttttggttt tcatgtacta gcgaaatga 1119

<210> 44

<211> 1029

<212> DNA

<213> 柱状田头菇

<400> 44

atgtctgcgt tgccctctca gttcaaactg cctgacctcc tctccacctg cccgttgaag 60

gacggcacca atcccgccta caagaaagcg gccgcagagt cacgagcatg gatcggcagc 120

tacaatatgt tcgccgacag aaagcgcgct ttctttattc agggacagaa cgagctcctt 180

tgctcccatg tctactgcta tgcgggttat gaacagctcc gtactacctg cgacttcgtg 240

aatctcctgt ttgttgttga cgaggtcagc gatgaacaaa gcggtgaaga cgcacgggcg 300

acgggccaag ttttcgtgaa cgccatgaaa tacgctgatt ggcacgatgg ctcaaaactc 360

gcgaagctca ctaaagactt ccgtgtgcgc ttcctacgcc tggccggacc caaaaatgtt 420

gcccgatttg ttgccctctg cgaaagttac actgcatgcg tcggcaaaga ggccgaattg 480

cgcgaaagcg gtcaagtcct cggcgtcaaa gaatttatcc ccttacggcg gcaaaacagc 540

gcagtcttgc tctgcttctc tcttgtggag tacattctcg gcatcgacct cgacgatgag 600

gtttaccggg atgagaactt tttgaacgcc tactgggcgg cttgtgacca cgtctgctgg 660

gcgaatgacg tctactccta cgacatggag cagtccaagg ggctgagcaa caacaacatc 720

gtcaccgtac tcatggaaga aaaccacacc tcgctgcaag acacctccga ttacattgga 780

gagaagtgcg cagagtttgt gcaaatttat ttgacctcca aaaagcgtct ctcgccatcc 840

ctcggtcctg atgcagccct tttcctcgaa tcgatagggt cgtggatggt tggtaacctt 900

gcgtggagtt tcgagacttc ccgctacttc ggttctcgtc acctcgaggt gaaagagaca 960

ggaatcgtca ttcttcgccc acgagaactt ccagaagacg gcagttcttc tgactcggat 1020

gaagagtaa 1029

<210> 45

<211> 1164

<212> DNA

<213> 柱状田头菇

<400> 45

atgagcttct tcaagtcttc gcagccgacg atctacattc ccgacaccct cagaaactgg 60

ccttggccca gggaaattaa cccccattac gaggaatgca agcgagagtc agccgcctgg 120

gttgagaagt tcggggcctt cagcgctaag gcccagaaag cgttcaacaa atgcgacttc 180

aatcttttgg cctcgcttgc atggtctcga gtaaaccgcg acggctgcag gattggatgc 240

gacttgatga atttgttctt cgtcttcgat gaatggtcgg acgtttccga cgcggaagag 300

acaagacgca tggccgacat cattatggac gccctctacg atcctcataa acctcgccca 360

actggagaat gggtcggtgg cgaagttact agacagtact ggcttaacgc catccgcact 420

gctactccct ctgctcagaa gaggttcatc aaggcgttca agctctacac cgactctgtt 480

gtccagcagt ccgccgaccg cgataagcac ctcattcgcg atatcgacag ttacttcgag 540

gtcaggcgcg acactatcgg cgcgaagccg tccttcgcca tcaacgaggt gcacatgaac 600

cttccggact atgttatgga gcacccggtc atcaagaacc tcacggcata ctgcatcgac 660

atgctttgca tcggcaacga tttgtgctct tacaacgtcg aacaaagccg cggggatgat 720

gggcacaacc ttgtcactat cgtcatgcac cagctcaacc tcgacgtcca aggcgcattc 780

gattggatcg gaaagctcca cgacgagtta gtcgacaagt tcctcgaaga atacaagaat 840

gtccctacgt ttaaggacaa acaagtcacc aaggaatgcg ctgagtatgc cttcggtctc 900

ggcaattggg tccgaggaaa cgaccaatgg agcttcgaga gtgagcgcta tttcaggaaa 960

gacggcatga gggtcctgac agagcggact gtcgttctcc tgcccaagaa gcgcgaaccg 1020

cccccgaagc ccctcgatga agatccaatc tacagcgctc tgccgtggtg gggttggacg 1080

gctctctttg gatttttggc cacagccttc acgttctccg cccgtcaact ctcaactcgt 1140

atttcggcga acctcattgc ttaa 1164

<210> 46

<211> 1170

<212> DNA

<213> 柱状田头菇

<400> 46

atggtttggg acttcgtact ctccctcttc cattcccttc tcgccgcgtt ccagaccttg 60

acatcctggc tgacgggtag tttcttgttc aacaacaaga tggccccagc tccaaatcca 120

gctcccgtta ccttcatcct tcccgacctc gaaaagacgt tcaactcgct acccgacgac 180

ggactcaacc cacatcacga cgtcgcttgc gcagagtcgc gtgaatggtt cgcaaaatac 240

aacaagaagg ttctcggtgc ccagatgcaa gagttcttca ggcgctgcaa gttcgaactc 300

atcacgtcgt acacctatcc ctacgtggac aaggaaggtc tcagagcgac tatggattgg 360

cacaatatcc tctggttctt cgacgaggtt acagacaccg aaactggcaa ggacgctcac 420

aaatccgcca tcatcactat ccgcaccctc cgcgagcccg acttcgatga cggatcatca 480

ctgtgcagaa tggtcaggga cttccgcctc agtcacctct ctcgcgccgg tcccgaatgt 540

actcgccgat tcctagagca ctgcgatgtt gcgtttcacg cgggtgcggt ggaagctgaa 600

ctccgtgaga aaggcgaagt gctctcgatc gaaggatacc tcaaactccg tcgcgagacg 660

agcggagcgc gcacctgctt cgatatggcc gaatacctca tggacattga ccttccccag 720

gacatgtatg acgaccccgt attccagaag ggctacatcg ctgctcttga cctcatcttc 780

ctcgctaacg atctttattc ttacaacatg gaacaagcta aggggcacaa cggtgccaac 840

gttctgaccg tcgtcatgaa agagaccaag ctgaacctcc aatccgctgc tgactacgtc 900

ggtgttcttt gtgagaagct catcaagcaa ttccaagagg caaagagcac gctcgagaat 960

cgcctcgcga aggagaagaa ccccgctaag gccgctgcgc tgaaggatgc aatccgctcg 1020

ttggtcgggt acggacactg ggttcggggc aacgtcgaat ggagcttcga gacagagaga 1080

tatttcggga agaagaacaa ggagatcaag aagtctcggg ttgtgaccct gacgcctacg 1140

aacagtgtta atcgcgctct caaggcctag 1170

<210> 47

<211> 1077

<212> DNA

<213> 柱状田头菇

<400> 47

atgaacgcat cgcctttctt gaacgagtct tcgccaacgc ggcccacgtc cttcgtgctt 60

cctgatctcg tctcgcactg caagttcccg ctttcttatc atcccaacgg ggacgagatc 120

gcgcaggagt ctgttgattg gctcgatagt agctgcccag acctgaccgc gaaacagcgg 180

cgtgcgcttc gtgtcctgca gtcgggcgaa ctcacagcct actgctataa ccaggcgacc 240

tccccagaac gcctacgcgt cgtgtccgac tttctgacat acctctttca tttggacaac 300

atcagcgatg gaatgatgac gcgcgagacg gatgtgctcg cagacgtcgt catgaatgcg 360

ttttggttca ccgataagta tatgccgact cggggccccg gaaaggaaca gctggacgaa 420

gagcttaatc ctggcaaact ggcacgagat ttctggtcgc gggctattgc agactgtgga 480

gttggagtgc aagcccggtt caaagaaaca atgggactgt tctttgaagc cgtcaacatc 540

caggctagaa tgcgagatga agacaccata ccggacttgg agtcgtatat cgatgtccgg 600

agagacacgt cagggtgcaa accatcttgg gtgctaatcg aatatgcact cggaattgat 660

cttccggacc atgtcgttga ccatccgata atgcaagcgc taaaccaagg tactaatgac 720

cttgtaacct ggtctaatga catattctct tataacgtcg aacaatcacg aggcgacacg 780

cataatatga ttgttattct tatggaatat catggtcata ccctgcagag tgctgttgat 840

tatgttggcg agttatgcgc gcaaaccatc gacacctttt gcgaaaacaa ggaacgtctt 900

ccgtcttggg gtcccgaaat cgacgatatg gttgctcgtt acgttaaggg tcttcaggac 960

tggattgttg gttctttgca ttggagtttt caaacccaac ggtatttcgg caaagacggc 1020

ttagacatca agaagcatag atttgtcaag ctcttacctt tggaagctgc aaaataa 1077

<210> 48

<211> 1341

<212> DNA

<213> 柱状田头菇

<400> 48

atgggtcgtt cttctgtggt tcgttcattt tttttgtcgg tacaagccat ggcctcttcc 60

ttgcttgagc catctttggc cgctatcgcg ctcgtcatct tgctcgcgtc tgtttccctc 120

tctcgaaaga agcgcccagc tgctccagag ccacaggggc tgtcggtgct cggcaatctt 180

ttcgacatcc cgaagagagc gtcatccata atttacctcg ccttaggaaa gccgtataat 240

acgctcacca agagagctgt ctcacaactt cagggttaca ctcctgggag tcacatcgac 300

gctacttccc actctccgcg tgtcttccgt ctcccgaatc tggaagagac attctccgtc 360

ttccccgacc atggcttgaa ccccaactac acgtccgccc gtaccgactc cagagcatgg 420

atcaatcagt ataccaaggt ggtctgtggg cccaagatgg tcgccttcat gaacaactgc 480

gaatttgagc tttctaactc tcactgctac ccgtacgccg gctacaaagg actcaaggct 540

acaatggatc ttacaaatat cctctggctc tatgacgagt ataccgatac cggatccggc 600

gcggaagctg tcaaggcggc aggtattgtt gctcgcgctc tgcgagagcc agactacgat 660

gatggaactt gggtctgccg catgatgaaa tccttcaaac aaaaccacat tgataaagcg 720

ggccctggtg tcgctcgccg cttcatcgac aacttctgca actacgttga agttgtcggt 780

agagaagcag agcttcgaga aaagaacgag gtgcttgaca tccccaacta cgtcactttc 840

cgtcgtgaga caagtgccgt tcgcacttgc ttcgacttgg tggaatattg cctcgatctt 900

gaccttcccc aatacgtgca cgatgatcct gtcttcatca gtggctacaa cgcaggcatg 960

gatctcgtct tctgggctaa cgacctcgtc tcttacaaca tggagcaatc taagggacac 1020

agtggagcca acgtcgtgac cgtgatcatg aagtccaaag gtgtcgatct tcaaacggcc 1080

gtcgatttct tgggaggata ctgcgaggca cttaccgcac agctgctcga ggcgaagcgc 1140

attcttcagg ctcgttccga tgctgcttac tccagagatg tagtccgcct tatggatgct 1200

ttcggtgatt gggtgagagg aaacgttgcg tggagctttg agacagagag gtatttcgga 1260

aaggaaaaca agagagtcaa ggagacactg cttgttgaac tcaaggagcc gttcgttggt 1320

gcactcgcct tgaaagagta a 1341

<210> 49

<211> 1155

<212> DNA

<213> 柱状田头菇

<400> 49

atgcactcaa gcactcaact attcactcac tcgccaacga agatgcaaat tatcctgcct 60

gacctcctct gctcttgggg gtacaaaggt ttcctgaacc cgcactatga aggcgccaag 120

gcagagtcca atacctggat acggcccttg gtcgttaaac tctttgatga gagaggacaa 180

aaagctttcg cgaaggacta caccggcctc cttgcgagta tgacgtaccc ccatcacaac 240

aaggaatttc tttgcgtcgc atgcgacatg atgaacctct tcttcgtgta cgatgagtac 300

acagacatca ccccaccaga aactgcacaa agactcgcca agattgtcgt caacgccatg 360

aggaaccccg acgagatagc cgccctcggc gaagactcca taggaactat gacgaagcag 420

ttctggagac gggcgatgac actcctgcca cccaacggat gtaattccga gtcatgcata 480

cagcacttca tcgattacac agaagagtac ttgaccgccg tcacacgcga agcttgcgat 540

cgtagctcgg gtagcgtcca tgccgtgaaa gactaccttg caatgcgacg agcgacgagc 600

ggcgccggcc tgatggtagg gctgctcgag tttggattgg acttgcctga agaagtcatg 660

aagcacgagg taatccaaga gctctccacc ggcgccatcg acatgtactg cctactgaac 720

gatatgcatt cgtatgcgag cgagctctct tccgggcagg cgagccacaa cgtcataaca 780

gttgtaatgc atgagaggaa tctttccctt caagaagcat ttgactggtt agcttcctac 840

gcagctggcg tggtcaaggg tttcaagacg aatctgaacc gagtgccatc gttttccgag 900

ctggaggacc actctttgca cggcgaaggg atgctgagag ataggataca gagatatatc 960

gatggcctcg gccaggcggt cagggcggag gacgactggg catttgagac gacgagatac 1020

catggacaga atggcccaca gatcaagctt acgaggatgt tgaccattcg acctcaggtc 1080

aaggacaatt acgcgaagca ggcgccgagg gtcgaggcgc ctgagaggcc tatgcttctg 1140

gaagtcagga gttga 1155

<210> 50

<211> 1887

<212> DNA

<213> 大肠杆菌

<400> 50

atgcatcatc atcaccatca cgagctcagt tttgatattg ccaaataccc gaccctggca 60

ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc 120

gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc 180

gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac cccgtttgac 240

caattgattt gggatgtggg gcatcaggct tatccgcata aaattttgac cggacgccgc 300

gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg gcgcggcgaa 360

agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt 420

attgcggttg ctgccgaaaa agaaggcaaa aatcgccgca ccgtctgtgt cattggcgat 480

ggcgcgatta ccgcaggcat ggcgtttgaa gcgatgaatc acgcgggcga tatccgtcct 540

gatatgctgg tgattctcaa cgacaatgaa atgtcgattt ccgaaaatgt cggcgcgctc 600

aacaaccatc tggcacagct gctttccggt aagctttact cttcactgcg cgaaggcggg 660

aaaaaagttt tctctggcgt gccgccaatt aaagagctgc tcaaacgcac cgaagaacat 720

attaaaggca tggtagtgcc tggcacgttg tttgaagagc tgggctttaa ctacatcggc 780

ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat gcgcgacctg 840

aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa 900

aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg ttgtttgccg 960

aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg 1020

gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg 1080

gtcgagtttt cacgtaaatt cccggatcgc tacttcgacg tggcaattgc cgagcaacac 1140

gcggtgacct ttgctgcggg tctggcgatt ggtgggtaca aacccattgt cgcgatttac 1200

tccactttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcgat tcaaaagctt 1260

ccggtcctgt tcgccatcga ccgcgcgggc attgttggtg ctgacggtca aacccatcag 1320

ggtgcttttg atctctctta cctgcgctgc ataccggaaa tggtcattat gaccccgagc 1380

gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga tggcccgtca 1440

gcggtgcgct acccgcgtgg caacgcggtc ggcgtggaac tgacgccgct ggaaaaacta 1500

ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggcgatcct taactttggt 1560

acgctgatgc cagaagcggc gaaagtcgcc gaatcgctga acgccacgct ggtcgatatg 1620

cgttttgtga aaccgcttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg 1680

ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gcagcggcgt gaacgaagtg 1740

ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt 1800

ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc tggtatggaa 1860

gccaaaatca aggcctggct ggcataa 1887

<210> 51

<211> 1881

<212> DNA

<213> 人工序列

<220>

<223> Ec.dxs_SL3

<400> 51

atgcatcatc atcaccatca cagttttgat attgccaaat acccgaccct ggcactggtc 60

gactccaccc aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa 120

ctgcgccgct atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg 180

ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca acaccccgtt tgaccaattg 240

atttgggatg tggggcatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa 300

atcggcacca tccgtcagaa aggcggtctg tacccattcc cgtggcgcgg cgaaagcgaa 360

tatgacgtat taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg 420

gttgctgccg aaaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg 480

attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg 540

ctggtgattc tcaacgacaa tgaaatgtcg atttccgaaa atgtcggcgc gctcaacaac 600

catctggcac agctgctttc cggtaagctt tactcttcac tgcgcgaagg cgggaaaaaa 660

gttttctctg gcgtgccgcc aattaaagag ctgctcaaac gcaccgaaga acatattaaa 720

ggcatggtag tgcctggcac gttgtttgaa gagctgggct ttaactacat cggcccggtg 780

gacggtcacg atgtgctggg gcttatcacc acgctaaaga acatgcgcga cctgaaaggc 840

ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac 900

ccgatcactt tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt 960

agcggcggtt tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg 1020

aaagacaaca agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag 1080

ttttcacgta aattcccgga tcgctacttc gacgtggcaa ttgccgagca acacgcggtg 1140

acctttgctg cgggtctggc gattggtggg tacaaaccca ttgtcgcgat ttactccact 1200

ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gcttccggtc 1260

ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca tcagggtgct 1320

tttgatctct cttacctgcg ctgcataccg gaaatggtca ttatgacccc gagcgatgaa 1380

aacgaatgtc gccagatgct ctataccggc tatcactata acgatggccc gtcagcggtg 1440

cgctacccgc gtggcaacgc ggtcggcgtg gaactgacgc cgctggaaaa actaccaatt 1500

ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga tccttaactt tggtacgctg 1560

atgccagaag cggcgaaagt cgccgaatcg ctgaacgcca cgctggtcga tatgcgtttt 1620

gtgaaaccgc ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc 1680

accgtagaag aaaacgccat tatgggcggc gcaggcagcg gcgtgaacga agtgctgatg 1740

gcccatcgta aaccagtacc cgtgctgaac attggcctgc cggacttctt tattccgcaa 1800

ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg ccgctggtat ggaagccaaa 1860

atcaaggcct ggctggcata a 1881

<210> 52

<211> 1860

<212> DNA

<213> 人工序列

<220>

<223> Ec.dxs_SL5

<400> 52

atgcatcatc atcaccatca cagttttgat attgccaaat acccgaccct ggcactggtc 60

gactccaccc aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa 120

ctgcgccgct atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg 180

ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca acaccccgtt tgaccaattg 240

atttgggatg tggggcatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa 300

atcggcacca tccgtcagaa aggcggtctg cacccgttcc cgtggcgcgg cgaaagcgaa 360

tatgacgtat taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg 420

gttgctgccg aaaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg 480

attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg 540

ctggtgattc tcaacgacaa tgaaatgtcg atttccgaaa atgtcggcgc gctcaacaac 600

catctggcac agctgctttc cggtaagctt tactcttcac tgcgcgatgg cgggaaaaaa 660

gttttctctg gcgtgccgcc aattaaagag ctgctcaaac gcaccgaaga acatattaaa 720

ggcatggtag tgcctggtac gttgtttgaa gagctgggct ttaactacat cggcccggtg 780

gacggtcacg atgtgctggg gcttatcacc acgctaaaga acatgcgcga cctgaaaggc 840

ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac 900

ccgatcactt tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt 960

agcggcggtt tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg 1020

aaagacaaca agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag 1080

ttttcacgta aattcccgga tcgctacttc gacgtggcaa ttgccgagca acacgcggtg 1140

acctttgctg cgggtctggc gattggtggg tacaaaccca ttgtcgcgat ttactccact 1200

ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gcttccggtc 1260

accttcgcca tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca tcagggtgct 1320

tttgatctct cttacctgcg ctgcataccg gaaatggtca ttatgacccc gagcgatgaa 1380

aacgaatgtc gcctgatgct ctataccggc tatcactata acgatggccc gtcagcggtg 1440

cgctacccgc gtggcaacgc ggtcggcgtg gaactgacgc cgctggaaaa actaccaatt 1500

ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga tccttaactt tggtacgctg 1560

atgccagaag cggcgaaagt cgccgaatcg ctgaacgcca cgctggtcga tatgcgtttt 1620

gtgaaaccgc ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc 1680

accgtagaag aaaacgccat tatgggcggc gcaggcagcg gcgtgaacga agtgctgatg 1740

gcccatcgta aaccagtacc cgtgctgaac attggcctgc cggacttctt tattccgcaa 1800

ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg ccgctggtat ggaagccaaa 1860

<210> 53

<211> 1173

<212> DNA

<213> 柱状田头菇

<400> 53

atgcatcacc atcaccatca ccacagctcg acccagctgt tcactcactc tccgaccaaa 60

atgcagatca tcctgccgga tctgctgtgc tcctggggat ataaagggtt tctcaaccct 120

cactatgagg gcgctaaggc ggaatccaac acctggattc gcccgctcgt agtgaaactt 180

ttcgacgaac gtggccagaa agcgttcgcc aaagattaca ccggtctgct ggcgtctatg 240

acttacccgc accacaacaa agaattcctg tgtgtggctt gcgatatgat gaacctgttc 300

tttgtttatg acgagtatac cgatattacc ccgccggaaa ccgcgcagcg tctggctaaa 360

atcgttgtaa atgctatgcg caatccggat gagatcgccg ctctgggtga agacagcatc 420

ggtactatga ccaagcagtt ctggcgccgc gctatgactc tgctgccacc gaacggttgt 480

aactctgaat cttgtatcca gcacttcatt gactacaccg aagaatatct gactgcagtg 540

acgcgtgaag cttgtgatcg tagcagcggc tctgtccacg ctgtaaaaga ctatctggcg 600

atgcgtcgtg caacctctgg cgcgggtctg atggttggtt tactggaatt tggcctcgat 660

ctgccggaag aagtcatgaa acatgaagtg atccaggaac tgtctactgg cgcgatcgac 720

atgtactgtc tgctgaacga catgcatagc tacgcatctg aactgtcgtc tggtcaggcc 780

tctcacaacg ttattaccgt cgtgatgcac gaacgtaacc tgtccctgca agaagctttt 840

gattggctgg cctcttacgc ggcaggcgta gtgaagggtt tcaaaaccaa cctgaaccgc 900

gttccttctt ttagcgaact ggaggaccac tctctgcacg gcgaaggcat gctccgtgat 960

cgcattcagc gttacatcga cggcctgggt caggcggttc gcgcggaaga tgattgggct 1020

tttgaaacta cccgctatca cggtcagaac ggcccgcaga ttaaactgac ccgtatgctg 1080

accatccgtc cgcaggttaa ggacaactac gctaaacagg ctccgcgcgt cgaggctcct 1140

gaacgcccga tgttactgga agtgcgtagt taa 1173

<210> 54

<211> 1050

<212> DNA

<213> 平田头菇

<400> 54

atgcatcacc atcaccatca ctcccaattt attatcccgg atctgttatc cacttggcct 60

tggcagcgcg tgtctaaccc gatgtggcgt gagattgatg aagaagcaaa cgcgtgggtg 120

cagtctttcg acctgtttga accgtcccag ttcgagaaat tcaagatgtg tgatttcaac 180

ctgctgggct cgatgattgg taccgtggaa accaaagatc atctgcgcat cagttgcgac 240

ctgatgaact tctatttcgc attcgacgaa tacacggaca tggcatccaa agacgaagcg 300

cgtaagatcg cccgtgacgt gatggatgca tttcgcaaca ccgaaaaacc agctcacaac 360

caaatcaccg aaatggcccg ccagtttttt aaacgtacga tcgacactgt aggtaaagac 420

ttgccgggcg tggaacgttt tatcgctgac ttcgatgctt atacgcattc cgtgatccag 480

gaggccgacg accgtgctac tggccacatc cgcagcgtca acgactattt cacgttacgc 540

cgcgacactt gcggcgcgaa accaagcttc agcttctttg gcctgggtct gaacattccg 600

gatgaggttt tccaccatcc tgtggttatt tccatgatcg aaggcgccac cgatctgatt 660

gctgttacga atgacatgca ctcatataac ctcgaacact cccgtggtct ggatggacac 720

aacgtaatca ctgctatcat gcacgaatac cagctggacc tgcagggcgc actgtactgg 780

ctatccggtt acgctaccca tacgattgct aacttcctgt cgaaccgccg taacctgccg 840

tcctggggcc cggctgtcga taaagcggta gaagaatttt tcgaccgtgt tggtcgttgt 900

gttcgtggct acgacgcttg gagctacgaa accaaacgct attacgggaa aaacggcctg 960

caggtgcaga agacccgtcg tatcaccctg cgcccgcgtg acgcgtctta cattactaag 1020

gagcagctgc aggtatctat caaagcataa 1050

<210> 55

<211> 1053

<212> DNA

<213> 平田头菇

<400> 55

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgttc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 56

<211> 1050

<212> DNA

<213> 纹缘盔孢伞

<400> 56

atgcatcacc atcaccatca cccgagccag ttcaccatcc ctgacctgct gatcacttgg 60

ccatggcagg aaatcaccaa cccgatgctg cacgaagtag acgctgaagc caatgaatgg 120

gttcagtcac tgaacctgtt cgaaccgaaa cagttcgaaa agttcaaagc ctgtaacttc 180

aacctgctgg gctccctggt tggaccgctg ccaagccgtg accaccttcg tgtgtcctgc 240

gacctgatga acttctactt cgctttcgat gagtacacgg acatggcaaa caaagatgaa 300

gctatgcgta tcgcccgtga cgtaatgcaa gcattccgta acactgacac gcctagcaac 360

tctaaaatca ctgaaatggc acgtcaattc ttcaaacgta ccatcgaggt tgtaggcgaa 420

gacctgccgg gcatcgaacg tttcatcgcc gatttcgatg catacactcg ttccgtgatt 480

caggaagcag atgatcgtgt tgcaggtcac attcgtaacg tggaggacta cttcattctg 540

cgccgtgata cgtgcggtgc taaaccgagc ttctctttct acggtctggg tctgaacatc 600

ccgaccgagg tctttgaaca cccgctgctg atttctatgg ttgaaagcgc aactgacctg 660

attgctgtca ccaatgacat gcactcttac ggtctggagc attctcgtgg cctggacggt 720

cacaatgtta tcaccgccat tatgcacgaa tatcagctgg acctacaggg tgccctgtac 780

tggctgtccg gctacgcaac taaaaccatc tccaaattcc tgactgatcg taaaaacctg 840

ccgtcctggg gcccaaccat tgacaaagct ctcgaaatct acctggaccg tttaggtcgt 900

tgcgtgcgtg gctacgatgc ttggtcctac agcactaagc gttactatgg taaaaacggt 960

ctgaaggttc aaaaaacccg ccgtatcact ttgaaaccgc gtgatgcagc ctacatcacg 1020

aaggatcagc tgcaggtgtc tatcgcttaa 1050

<210> 57

<211> 1053

<212> DNA

<213> 砖红韧黑伞

<400> 57

atgcatcacc atcaccatca ctccgctcag tacaccatcc ctgacttact ggcgaactgg 60

ccgtggcagc gcgtgaccaa cagcatgctg gacgaggtgc gcgacgaagc aaacgagtgg 120

gtgatgtccc tgggcctgtt tgagccggct cagtttaaaa aattcaaagc atgcgacttc 180

aaccttctgg caagctttat tggtccgctg gaaagtaaag aacaccttcg tgttgcttgc 240

gatctgatga atttctattt cgctttcgat gagtacactg acgttgctaa ccgtgaagag 300

gctaaaaaaa tctctcaggg cgttatgcac gcgtttaaaa ctcgttctgc agagccgtcc 360

agctccaaaa ttacggaaat ggcgcgtcag ttctttcgtc gtaccgttga cgtcgtcggt 420

gaagattccc cggccatcaa ccagttcatt accgacttcg acacctacac cacggctgtg 480

atccaggaag cagacgatcg caccgaaggt accatccgta atgttgagga ctatttcacc 540

ctccgtcgcg acacctgcgg cgccaaacct tctttttcgt tcttcgccct gggcctgaac 600

atgccgaccg aagtcttcga acaccctctg atcatgtctc tggtagagcg tgcaaccgac 660

ctgatcgcga ttgtgaacga catgcactcc tacggtctgg aacgtgctcg tggcctggat 720

ggtcataacg tagtaacgtc catcatgtac gagcatcagc tggacctgca gggtgcgctg 780

cactggctgg ccggttatgc cgaagacaca atcgccaagt tcctgtcgga aaaagaacgt 840

ctcccgtcct ggggtccggc ggtagatgta tccgtgcagg aatttgttga ccgtctgggc 900

cgttgcgtgc gtggttacga cgcttggtcc tatgaaacga accgctatta cggcagccac 960

ggtctacaga ttcgtcagac tcgtcaaatc accctgccat ccaacgactc tggctacatc 1020

actcgcaagc agatgggcgt ttctatcgct taa 1053

<210> 58

<211> 1029

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut1

<400> 58

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgttc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaataa 1029

<210> 59

<211> 996

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut2

<400> 59

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgttc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgtaa 996

<210> 60

<211> 930

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut3

<400> 60

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgttc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttaa 930

<210> 61

<211> 993

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut4

<400> 61

atgcatcacc atcaccatca ctggccgtgg caaaaggtgc gcaacccact gctgcaagaa 60

gtgcaggatg aggcgaacga atgggtgaaa tctttcgtgc tgttcgaacc ggaacagttt 120

gagaaattta aggcatgtga tttcaacctg ctgggtgcac tggttggtcc gctgggtacc 180

aaggaagaac tgcgcattag ctgtgacctg atgaacttct acttcgcctt cgatgagtac 240

accgacctgg ctagcgctga cgaagccaaa gtaatcgcgc gcgatgtgat ggaaagcttc 300

cgtcacacag ataagccaag ccacaacaaa attactgaaa tggcccgtca attcttcgaa 360

cgtactatca acacggttgg taacgatccg actggcattg aacaatttat tgcggatttc 420

gacgcataca ccacctctat catccaagaa gctgatgatc gtgcgtctgg tcacattcgt 480

agcgttgagg attacttcat tctgcgccgt gacacctgtg gtgggaaacc gtcgttctcg 540

ttcttcggtc tgggcctgaa catcccgaaa gaagtcttcg cacacccgat gttcatctcc 600

atgaccgaat ccgcgactga tctgatcgca attaccaacg atatgcactc ttataacctg 660

gagcaatccc gcggcttgga tggtcacaat gtaattaccg cgatcatgca cgaatacaaa 720

attaatctcc agggtgcgct gtactggctg tcgggttacg ctactaaaac tattgcgaaa 780

tttatctctg accgtaaaaa tttgccgagc tggggcccag tggttgaccg cgccgttgaa 840

caatactttg accgtgttgg tcgttgcgtt cgtggctacg atgcctggtc ttacgaaacc 900

aaacgttatt acggtaaaaa tggcctggaa atccagaaaa ctcgtcagat caccctgcgc 960

ccgctggacc cggcgtacgt gaccaaagaa taa 993

<210> 62

<211> 946

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut5

<400> 62

atgcatcacc atcaccatca cgaggcgaac gaatgggtga aatctttcgt gctgttcgaa 60

ccggaacagt ttgagaaatt taaggcatgt gatttcaacc tgctgggtgc actggttggt 120

ccgctgggta ccaaggaaga actgcgcatt agctgtgacc tgatgaactt ctacttcgcc 180

ttcgatgagt acaccgacct ggctagcgct gacgaagcca aagtaatcgc gcgcgatgtg 240

atggaaagct tccgtcacac agataagcca agccacaaca aaattactga aatggcccgt 300

caattcttcg aacgtactat caacacggtt ggtaacgatc cgactggcat tgaacaattt 360

attgcggatt tcgacgcata caccacctct atcatccaag aagctgatga tcgtgcgtct 420

ggtcacattc gtagcgttga ggattacttc attctgcgcc gtgacacctg tggtgggaaa 480

ccgtcgttct cgttcttcgg tctgggcctg aacatcccga aagaagtctt cgcacacccg 540

atgttcatct ccatgaccga atccgcgact gatctgatcg caattaccaa cgatatgcac 600

tcttataacc tggagcaatc ccgcggcttg gatggtcaca atgtaattac cgcgatcatg 660

cacgaataca aaattaatct ccagggtgcg ctgtactggc tgtcgggtta cgctactaaa 720

actattgcga aatttatctc tgaccgtaaa aatttgccga gctggggccc agtggttgac 780

cgcgccgttg aacaatactt tgaccgtgtt ggtcgttgcg ttcgtggcta cgatgcctgg 840

tcttacgaaa ccaaacgtta ttacggtaaa aatggcctgg aaatccagaa aactcgtcag 900

atcaccctgc gcccgctgga cccggcgtac gtgaccaaag aataaa 946

<210> 63

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut6

<400> 63

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgggc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 64

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut7

<400> 64

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatggtc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 65

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut8

<400> 65

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgatc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 66

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut9

<400> 66

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatggac atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 67

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut10

<400> 67

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgctc atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 68

<211> 1053

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut11

<400> 68

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta cttcattctg 540

cgccgtgaca cctgtggtgg gaaaccgtcg ttctcgttct tcggtctggg cctgaacatc 600

ccgaaagaag tcttcgcaca cccgatgcgt atctccatga ccgaatccgc gactgatctg 660

atcgcaatta ccaacgatat gcactcttat aacctggagc aatcccgcgg cttggatggt 720

cacaatgtaa ttaccgcgat catgcacgaa tacaaaatta atctccaggg tgcgctgtac 780

tggctgtcgg gttacgctac taaaactatt gcgaaattta tctctgaccg taaaaatttg 840

ccgagctggg gcccagtggt tgaccgcgcc gttgaacaat actttgaccg tgttggtcgt 900

tgcgttcgtg gctacgatgc ctggtcttac gaaaccaaac gttattacgg taaaaatggc 960

ctggaaatcc agaaaactcg tcagatcacc ctgcgcccgc tggacccggc gtacgtgacc 1020

aaagaacagc tgcaggtatc catgaaggca taa 1053

<210> 69

<211> 996

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut12

<400> 69

atgcatcacc atcaccatca ctcctctcag atctatatcc cggacctcct gatcacctgg 60

ccgtggcaaa aggtgcgcaa cccactgctg caagaagtgc aggatgaggc gaacgaatgg 120

gtgaaatctt tcgtgctgtt cgaaccggaa cagtttgaga aatttaaggc atgtgatttc 180

aacctgctgg gtgcactggt tggtccgctg ggtaccaagg aagaactgcg cattagctgt 240

gacctgatga acttctactt cgccttcgat gagtacaccg acctggctag cgctgacgaa 300

gccaaagtaa tcgcgcgcga tgtgatggaa agcttccgtc acacagataa gccaagccac 360

aacaaaatta ctgaaatggc ccgtcaattc ttcgaacgta ctatcaacac ggttggtaac 420

gatccgactg gcattgaaca atttattgcg gatttcgacg catacaccac ctctatcatc 480

caagaagctg atgatcgtgc gtctggtcac attcgtagcg ttgaggatta tttcacgtta 540

cgccgcgaca cttgcggcgc gaaaccaagc ttcagcttct ttggcctggg tctgaacatt 600

ccggatgagg ttttccacca tcctgtggtt atttccatga tcgaaggcgc caccgatctg 660

attgctgtta cgaatgacat gcactcatat aacctcgaac actcccgtgg tctggatgga 720

cacaacgtaa tcactgctat catgcacgaa taccagctgg acctgcaggg cgcactgtac 780

tggctatccg gttacgctac ccatacgatt gctaacttcc tgtcgaaccg ccgtaacctg 840

ccgtcctggg gcccggctgt cgataaagcg gtagaagaat ttttcgaccg tgttggtcgt 900

tgtgttcgtg gctacgacgc ttggagctac gaaaccaaac gctattacgg gaaaaacggc 960

ctgcaggtgc agaagacccg tcgtatcacc ctgtaa 996

<210> 70

<211> 1050

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689675_mut13

<400> 70

atgcatcacc atcaccatca ctcccaattt attatcccgg atctgttatc cacttggcct 60

tggcagcgcg tgtctaaccc gatgtggcgt gagattgatg aagaagcaaa cgcgtgggtg 120

cagtctttcg acctgtttga accgtcccag ttcgagaaat tcaagatgtg tgatttcaac 180

ctgctgggct cgatgattgg taccgtggaa accaaagatc atctgcgcat cagttgcgac 240

ctgatgaact tctatttcgc attcgacgaa tacacggaca tggcatccaa agacgaagcg 300

cgtaagatcg cccgtgacgt gatggatgca tttcgcaaca ccgaaaaacc agctcacaac 360

caaatcaccg aaatggcccg ccagtttttt aaacgtacga tcgacactgt aggtaaagac 420

ttgccgggcg tggaacgttt tatcgctgac ttcgatgctt atacgcattc cgtgatccag 480

gaggccgacg accgtgctac tggccacatc cgcagcgtca acgactactt cattctgcgc 540

cgtgacacct gtggtgggaa accgtcgttc tcgttcttcg gtctgggcct gaacatcccg 600

aaagaagtct tcgcacaccc gatgttcatc tccatgaccg aatccgcgac tgatctgatc 660

gcaattacca acgatatgca ctcttataac ctggagcaat cccgcggctt ggatggtcac 720

aatgtaatta ccgcgatcat gcacgaatac aaaattaatc tccagggtgc gctgtactgg 780

ctgtcgggtt acgctactaa aactattgcg aaatttatct ctgaccgtaa aaatttgccg 840

agctggggcc cagtggttga ccgcgccgtt gaacaatact ttgaccgtgt tggtcgttgc 900

gttcgtggct acgatgcctg gtcttacgaa accaaacgtt attacggtaa aaatggcctg 960

gaaatccaga aaactcgtca gatcaccctg cgcccgctgg acccggcgta cgtgaccaaa 1020

gaacagctgc aggtatccat gaaggcataa 1050

<210> 71

<211> 1089

<212> DNA

<213> 人工序列

<220>

<223> AAE3_109435_mut1

<400> 71

atgcatcacc atcaccatca ccacagctcg acccagctgt tcactcactc tccgaccaaa 60

atgcagatca tcctgccgga tctgctgtgc tcctggggat ataaagggtt tctcaaccct 120

cactatgagg gcgctaaggc ggaatccaac acctggattc gcccgctcgt agtgaaactt 180

ttcgacgaac gtggccagaa agcgttcgcc aaagattaca ccggtctgct ggcgtctatg 240

acttacccgc accacaacaa agaattcctg tgtgtggctt gcgatatgat gaacctgttc 300

tttgtttatg acgagtatac cgatattacc ccgccggaaa ccgcgcagcg tctggctaaa 360

atcgttgtaa atgctatgcg caatccggat gagatcgccg ctctgggtga agacagcatc 420

ggtactatga ccaagcagtt ctggcgccgc gctatgactc tgctgccacc gaacggttgt 480

aactctgaat cttgtatcca gcacttcatt gactacaccg aagaatatct gactgcagtg 540

acgcgtgaag cttgtgatcg tagcagcggc tctgtccacg ctgtaaaaga ctatctggcg 600

atgcgtcgtg caacctctgg cgcgggtctg atggttggtt tactggaatt tggcctcgat 660

ctgccggaag aagtcatgaa acatgaagtg atccaggaac tgtctactgg cgcgatcgac 720

atgtactgtc tgctgaacga catgcatagc tacgcatctg aactgtcgtc tggtcaggcc 780

tctcacaacg ttattaccgt cgtgatgcac gaacgtaacc tgtccctgca agaagctttt 840

gattggctgg cctcttacgc ggcaggcgta gtgaagggtt tcaaaaccaa cctgaaccgc 900

gttccttctt ttagcgaact ggaggaccac tctctgcacg gcgaaggcat gctccgtgat 960

cgcattcagc gttacatcga cggcctgggt caggcggttc gcgcggaaga tgattgggct 1020

tttgaaacta cccgctatca cggtcagaac ggcccgcaga ttaaactgac ccgtatgctg 1080

accatctaa 1089

<210> 72

<211> 993

<212> DNA

<213> 人工序列

<220>

<223> Agrped1_689671_mut1

<400> 72

atgcatcacc atcaccatca ctcccaattt attatcccgg atctgttatc cacttggcct 60

tggcagcgcg tgtctaaccc gatgtggcgt gagattgatg aagaagcaaa cgcgtgggtg 120

cagtctttcg acctgtttga accgtcccag ttcgagaaat tcaagatgtg tgatttcaac 180

ctgctgggct cgatgattgg taccgtggaa accaaagatc atctgcgcat cagttgcgac 240

ctgatgaact tctatttcgc attcgacgaa tacacggaca tggcatccaa agacgaagcg 300

cgtaagatcg cccgtgacgt gatggatgca tttcgcaaca ccgaaaaacc agctcacaac 360

caaatcaccg aaatggcccg ccagtttttt aaacgtacga tcgacactgt aggtaaagac 420

ttgccgggcg tggaacgttt tatcgctgac ttcgatgctt atacgcattc cgtgatccag 480

gaggccgacg accgtgctac tggccacatc cgcagcgtca acgactattt cacgttacgc 540

cgcgacactt gcggcgcgaa accaagcttc agcttctttg gcctgggtct gaacattccg 600

gatgaggttt tccaccatcc tgtggttatt tccatgatcg aaggcgccac cgatctgatt 660

gctgttacga atgacatgca ctcatataac ctcgaacact cccgtggtct ggatggacac 720

aacgtaatca ctgctatcat gcacgaatac cagctggacc tgcagggcgc actgtactgg 780

ctatccggtt acgctaccca tacgattgct aacttcctgt cgaaccgccg taacctgccg 840

tcctggggcc cggctgtcga taaagcggta gaagaatttt tcgaccgtgt tggtcgttgt 900

gttcgtggct acgacgcttg gagctacgaa accaaacgct attacgggaa aaacggcctg 960

caggtgcaga agacccgtcg tatcaccctg taa 993

<210> 73

<211> 996

<212> DNA

<213> 人工序列

<220>

<223> Galma_223690_mut1

<400> 73

atgcatcacc atcaccatca cccgagccag ttcaccatcc ctgacctgct gatcacttgg 60

ccatggcagg aaatcaccaa cccgatgctg cacgaagtag acgctgaagc caatgaatgg 120

gttcagtcac tgaacctgtt cgaaccgaaa cagttcgaaa agttcaaagc ctgtaacttc 180

aacctgctgg gctccctggt tggaccgctg ccaagccgtg accaccttcg tgtgtcctgc 240

gacctgatga acttctactt cgctttcgat gagtacacgg acatggcaaa caaagatgaa 300

gctatgcgta tcgcccgtga cgtaatgcaa gcattccgta acactgacac gcctagcaac 360

tctaaaatca ctgaaatggc acgtcaattc ttcaaacgta ccatcgaggt tgtaggcgaa 420

gacctgccgg gcatcgaacg tttcatcgcc gatttcgatg catacactcg ttccgtgatt 480

caggaagcag atgatcgtgt tgcaggtcac attcgtaacg tggaggacta cttcattctg 540

cgccgtgata cgtgcggtgc taaaccgagc ttctctttct acggtctggg tctgaacatc 600

ccgaccgagg tctttgaaca cccgctgctg atttctatgg ttgaaagcgc aactgacctg 660

attgctgtca ccaatgacat gcactcttac ggtctggagc attctcgtgg cctggacggt 720

cacaatgtta tcaccgccat tatgcacgaa tatcagctgg acctacaggg tgccctgtac 780

tggctgtccg gctacgcaac taaaaccatc tccaaattcc tgactgatcg taaaaacctg 840

ccgtcctggg gcccaaccat tgacaaagct ctcgaaatct acctggaccg tttaggtcgt 900

tgcgtgcgtg gctacgatgc ttggtcctac agcactaagc gttactatgg taaaaacggt 960

ctgaaggttc aaaaaacccg ccgtatcact ttgtaa 996

<210> 74

<211> 999

<212> DNA

<213> 人工序列

<220>

<223> Hypsu_148385_mut1

<400> 74

atgcatcacc atcaccatca ctccgctcag tacaccatcc ctgacttact ggcgaactgg 60

ccgtggcagc gcgtgaccaa cagcatgctg gacgaggtgc gcgacgaagc aaacgagtgg 120

gtgatgtccc tgggcctgtt tgagccggct cagtttaaaa aattcaaagc atgcgacttc 180

aaccttctgg caagctttat tggtccgctg gaaagtaaag aacaccttcg tgttgcttgc 240

gatctgatga atttctattt cgctttcgat gagtacactg acgttgctaa ccgtgaagag 300

gctaaaaaaa tctctcaggg cgttatgcac gcgtttaaaa ctcgttctgc agagccgtcc 360

agctccaaaa ttacggaaat ggcgcgtcag ttctttcgtc gtaccgttga cgtcgtcggt 420

gaagattccc cggccatcaa ccagttcatt accgacttcg acacctacac cacggctgtg 480

atccaggaag cagacgatcg caccgaaggt accatccgta atgttgagga ctatttcacc 540

ctccgtcgcg acacctgcgg cgccaaacct tctttttcgt tcttcgccct gggcctgaac 600

atgccgaccg aagtcttcga acaccctctg atcatgtctc tggtagagcg tgcaaccgac 660

ctgatcgcga ttgtgaacga catgcactcc tacggtctgg aacgtgctcg tggcctggat 720

ggtcataacg tagtaacgtc catcatgtac gagcatcagc tggacctgca gggtgcgctg 780

cactggctgg ccggttatgc cgaagacaca atcgccaagt tcctgtcgga aaaagaacgt 840

ctcccgtcct ggggtccggc ggtagatgta tccgtgcagg aatttgttga ccgtctgggc 900

cgttgcgtgc gtggttacga cgcttggtcc tatgaaacga accgctatta cggcagccac 960

ggtctacaga ttcgtcagac tcgtcaaatc accctgtaa 999

<210> 75

<211> 555

<212> PRT

<213> 番茄

<400> 75

Met Ala Pro Ala Ala Ala Leu Met Ser Lys Cys Gln Glu Glu Glu Glu

1 5 10 15

Ile Val Arg Pro Val Ala Asp Phe Ser Pro Ser Leu Trp Gly Asp Arg

20 25 30

Phe His Ser Phe Ser Leu Asp Asn Gln Val Ala Glu Lys Tyr Val Glu

35 40 45

Glu Ile Glu Thr Leu Lys Glu Gln Thr Arg Ser Met Leu Met Ser Gly

50 55 60

Lys Thr Leu Ala Glu Lys Leu Asn Leu Ile Asp Ile Val Glu Arg Leu

65 70 75 80

Gly Ile Ala Tyr His Phe Glu Lys Gln Ile Asp Asp Met Leu Asn His

85 90 95

Ile Phe Asn Ile Asp Pro Asn Phe Glu Ala His Glu Tyr Asn Asp Leu

100 105 110

Cys Thr Leu Ser Leu Gln Phe Arg Ile Leu Arg Gln His Gly Tyr Tyr

115 120 125

Ile Ser Pro Lys Ile Phe Ser Arg Phe Gln Asp Ala Asn Gly Lys Phe

130 135 140

Lys Glu Ser Leu Cys Asp Asp Ile Arg Gly Ile Leu Asn Leu Tyr Glu

145 150 155 160

Ala Ser His Val Arg Thr His Gly Glu Asp Thr Leu Glu Glu Ala Leu

165 170 175

Ala Phe Ser Thr Ala His Leu Glu Ser Ala Ala Pro His Leu Lys Ser

180 185 190

Pro Leu Ser Lys Gln Val Thr His Ala Leu Glu Gln Ser Leu His Lys

195 200 205

Ser Ile Pro Arg Val Glu Thr Arg Tyr Phe Ile Ser Ile Tyr Glu Glu

210 215 220

Glu Glu Leu Lys Asn Asp Val Phe Leu Arg Phe Ala Lys Leu Asp Phe

225 230 235 240

Asn Leu Leu Gln Met Leu His Lys Gln Glu Leu Ser Glu Val Ser Arg

245 250 255

Trp Trp Lys Asp Leu Asp Phe Val Thr Thr Leu Pro Tyr Ala Arg Asp

260 265 270

Arg Ala Val Glu Cys Tyr Phe Trp Thr Met Gly Val Tyr Ala Glu Pro

275 280 285

Gln Tyr Ser Gln Ala Arg Val Met Leu Ala Lys Thr Ile Ala Met Ile

290 295 300

Ser Ile Val Asp Asp Thr Phe Asp Ala Tyr Gly Ile Val Lys Glu Leu

305 310 315 320

Glu Val Tyr Thr Asp Ala Ile Gln Arg Trp Asp Val Ser Gln Ile Asp

325 330 335

Arg Leu Pro Glu Tyr Met Lys Ile Ser Tyr Lys Ala Leu Leu Asp Leu

340 345 350

Tyr Asn Asp Tyr Glu Thr Glu Leu Ser Asn Asp Gly Arg Ser Asp Val

355 360 365

Val Gln Tyr Ala Lys Glu Arg Met Lys Glu Ile Val Arg Asn Tyr Phe

370 375 380

Val Glu Ala Lys Trp Phe Ile Glu Gly Tyr Met Pro Pro Val Ser Glu

385 390 395 400

Tyr Leu Ser Asn Ala Leu Ala Thr Ser Thr Tyr Tyr Leu Leu Thr Thr

405 410 415

Thr Ser Tyr Leu Gly Met Lys Ser Ala Thr Lys Lys Asp Phe Glu Trp

420 425 430

Leu Ala Lys Asn Pro Lys Ile Leu Glu Ala Asn Val Thr Leu Cys Arg

435 440 445

Val Ile Asp Asp Ile Ala Thr Tyr Glu Val Glu Lys Gly Arg Gly Gln

450 455 460

Ile Ala Thr Gly Ile Glu Cys Tyr Met Arg Asp Tyr Gly Val Ser Thr

465 470 475 480

Gln Val Ala Met Asp Lys Phe Gln Glu Met Ala Glu Thr Ala Trp Lys

485 490 495

Asp Val Asn Glu Gly Ile Leu Arg Pro Thr Pro Val Ser Ala Lys Ile

500 505 510

Leu Thr Arg Ile Leu Asn Leu Ala Arg Ile Ile Asp Val Thr Tyr Lys

515 520 525

His Asn Gln Asp Gly Tyr Thr His Pro Glu Lys Val Leu Lys Pro His

530 535 540

Ile Ile Ala Leu Leu Val Asp Ser Ile Glu Ile

545 550 555

<210> 76

<211> 567

<212> PRT

<213> 五脉白千层

<400> 76

Met Ser Gln Val Ser Ala Ile Pro Thr Thr Ser Pro Asn Lys Gly Thr

1 5 10 15

Gly Asp Val Ile Glu Arg Arg Ser Ala Gly Tyr His Pro Ser Val Trp

20 25 30

Gly Asp Tyr Phe Leu Lys Tyr Asp Ser Pro Ser Asn Ser Val Lys Phe

35 40 45

Glu Phe Leu Gly Arg Val Glu Glu Gln Ile Glu Glu Leu Lys Gly Glu

50 55 60

Val Arg Lys Met Leu Ala Gly Ala Val Asp Lys Pro Trp Gln Met Leu

65 70 75 80

His Leu Ile Asp Gln Ile Gln Arg Leu Gly Ile Glu Tyr His Phe Glu

85 90 95

Arg Glu Leu Asp Glu His Leu Glu Arg Ile His Lys Ser Phe Ser Gln

100 105 110

Leu Thr His Gly Tyr Phe Lys Gly Asp Asp Leu Arg Met Ile Ser Leu

115 120 125

Leu Phe Arg Leu Leu Arg Gln Gln Gly Tyr Asn Ile Ser Ser Glu Val

130 135 140

Phe Asn Lys Phe Lys Asp Ser Glu Gly Asn Phe Gly Glu Ser Leu Ala

145 150 155 160

Thr Asp Leu Arg Gly Leu Leu Ser Leu Tyr Glu Ala Cys His Leu Arg

165 170 175

Cys His Gly Asp Ile Ile Leu Asp Glu Ala Leu Pro Phe Ala Ile Ser

180 185 190

His Leu Glu Ser Ile Asp Glu Ser Lys Ala Gly Ala Asn Leu Ala Lys

195 200 205

Gln Val Asn His Ala Leu Lys Gln Pro Leu Arg Arg Gly Leu Pro Arg

210 215 220

Leu Glu Ala Arg Arg Tyr Ile Pro Leu Tyr Glu Glu Glu Pro Ser His

225 230 235 240

Asp Lys Val Leu Leu Ala Leu Ala Lys Leu Asp Phe Asn Leu Leu Gln

245 250 255

Glu Gln His Gln Lys Glu Leu Gly Asn Val Ser Arg Trp Trp Lys Asp

260 265 270

Ile Asp Val Pro Arg Lys Phe Pro Phe Ala Arg Asp Arg Ile Ala Glu

275 280 285

Leu Phe Phe Trp Ala Cys Gly Val Tyr Phe Glu Pro Glu Phe Ser Val

290 295 300

Ala Arg Val Ile Gln Ala Lys Ala Phe Ala Met Thr Ser Ile Leu Asp

305 310 315 320

Asp Ile Tyr Asp Val Tyr Gly Thr Leu Glu Glu Leu Val Leu Leu Thr

325 330 335

Glu Ala Ile Glu Lys Trp Asp Val Asp Ala Met Asp Gly Leu Pro Glu

340 345 350

Tyr Met Gln Ala Phe Tyr Lys Glu Leu Leu His Phe Tyr Glu Glu Val

355 360 365

Gly Asn Glu Val Ala Ala Lys Gly Arg Ser Tyr Arg Leu Val Tyr Ala

370 375 380

Lys Glu Val Met Lys Lys Leu Ala Arg Ala Tyr Tyr Gln Glu Ala Lys

385 390 395 400

Trp Phe His Thr Asn Tyr Thr Pro Thr Leu Glu Glu Tyr Met Pro Leu

405 410 415

Gln Leu Ile Thr Thr Gly Tyr Gly Met Met Ala Thr Thr Ser Leu Val

420 425 430

Gly Met Asp Asp Val Val Pro Lys His Val Phe Glu Trp Ser Ile Gly

435 440 445

Asp Cys Lys Ile Val Lys Ala Ala Gln Thr Ile Cys Arg Leu Met Asp

450 455 460

Asp Ile Ser Ser His Glu Phe Glu Gln Lys Arg Gly His Leu Val Ser

465 470 475 480

Ser Val Glu Leu Leu Met Lys Glu Arg Ser Leu Ser Glu Arg Glu Ala

485 490 495

Gly Glu Glu Leu Gln Lys Gly Val Ile Asp Ala Trp Lys Asp Thr Asn

500 505 510

Glu Glu Phe Leu Arg Pro Thr Ala Val Pro Met Lys Ile Leu Thr Arg

515 520 525

Val Leu Asn Leu Ser Arg Ala Met Asp Val Leu Tyr Ser Asp Gly Asp

530 535 540

Asn Tyr Thr His Ser Gly Thr Lys Leu Lys Asp Phe Val Thr Ser Leu

545 550 555 560

Phe Val Ser Pro Leu Pro Val

565

<210> 77

<211> 354

<212> PRT

<213> 纹缘盔孢伞

<400> 77

Met Ser Glu Gln Gln Tyr Arg Leu Pro Asp Leu Leu Cys Asp Trp Pro

1 5 10 15

Trp Thr Arg Tyr Ser Ser Pro His Tyr Pro Lys Ala Lys Lys Glu Ser

20 25 30

Ser Asp Trp Val Asn Ser Phe His Pro Phe Ser Ala Arg Gly Gln Lys

35 40 45

Gly Phe Glu Ala Cys Asp Leu Asn Leu Leu Ala Ser Leu Thr Tyr Ser

50 55 60

Arg Arg Asn Glu Glu Phe Val Arg Ala Gly Cys Asp Leu Met Asn Phe

65 70 75 80

Tyr Phe Val Tyr Asp Glu Tyr Thr Asp Val Ser Asp Pro Val Ser Ala

85 90 95

Ala Asn Leu Ala Asn Ile Val Ile Asp Ala Met Lys Asn Pro Asp Ala

100 105 110

Phe Pro Ser Asn Gly Ser His Leu Leu Gly Glu Met Thr Arg Gln Phe

115 120 125

Trp Arg Arg Ala Ser Thr Leu Ala Lys Pro Gly Ser Pro Cys Phe Glu

130 135 140

His Phe Ile Ala Thr Ser Glu Thr Tyr Leu His Ala Val Thr Gln Glu

145 150 155 160

Ala Glu Asp Arg Ala Ser Glu Arg Ile Arg Ser Val Asp Asp Tyr Leu

165 170 175

Asp Leu Arg Arg Asp Thr Cys Gly Ala Arg Pro Thr Leu Ala Phe Ile

180 185 190

Glu Phe Gly Leu Glu Leu Pro Ala Glu Val Thr Ser His Pro Val Ile

195 200 205

Val Ser Leu Thr Glu Ala Ala Val Asp Leu Ile Ile Leu Val Asn Asp

210 215 220

Met His Ser Tyr Thr Arg Glu Ile Ser Cys Gly Leu Ala Asn His Asn

225 230 235 240

Ile Ile Thr Ala Ile Met His Glu Tyr Asn Leu Asp Leu Gln Ser Ala

245 250 255

Phe Asp Cys Leu Asp Ala Tyr Ala Asn Glu Val Val Thr Arg Phe Leu

260 265 270

Ser Asp Leu Asp Arg Val Pro Cys Trp Gly Asp Asp Val Asp Glu Arg

275 280 285

Val Trp Met Tyr Val Asp Gly Leu Gly Gln Trp Val Arg Gly Asn Asp

290 295 300

Asp Trp Ser Cys Glu Gly Lys Arg Tyr Tyr Gly Asp Asp Gly Leu Arg

305 310 315 320

Ile Met Glu Thr Arg Leu Val Ser Leu Lys Leu Pro Lys Val Arg Arg

325 330 335

Arg Met Ala Arg Lys Trp Gly Trp Tyr Gly Lys Val Arg Gln Lys Phe

340 345 350

Ser Ala

<210> 78

<211> 372

<212> PRT

<213> 香灰菌

<400> 78

Met Arg Pro Ile Thr Cys Ser Phe Asp Pro Val Gly Ile Ser Phe Gln

1 5 10 15

Thr Glu Ser Lys Gln Glu Asn Phe Glu Phe Leu Arg Glu Ala Ile Ser

20 25 30

Arg Ser Val Pro Gly Leu Glu Asn Cys Asn Val Phe Asp Pro Arg Ser

35 40 45

Leu Gly Val Pro Trp Pro Thr Ser Phe Pro Ala Ala Ala Gln Ser Lys

50 55 60

Tyr Trp Lys Asp Ala Glu Glu Ala Ala Ala Glu Leu Met Asp Gln Ile

65 70 75 80

Val Ala Ala Ala Pro Gly Glu Gln Gly Ser Leu Pro Ala Glu Leu Ala

85 90 95

Val Ser Asp Lys Lys Ala Ala Lys Arg Arg Glu Leu Leu Asp Thr Ser

100 105 110

Val Ser Ala Pro Met Asn Met Phe Pro Ala Ala Asn Ala Pro Arg Ala

115 120 125

Arg Ile Met Ala Lys Ala Asn Leu Leu Ile Phe Met His Asp Asp Val

130 135 140

Cys Glu Tyr Gln Ser Val Gln Ser Thr Ile Ile Asp Ser Ala Leu Ala

145 150 155 160

Asp Thr Ser Thr Pro Asn Gly Lys Gly Ala Asp Ile Leu Trp Gln Asn

165 170 175

Arg Ile Phe Lys Glu Phe Ser Glu Glu Thr Asn Arg Glu Asp Pro Val

180 185 190

Val Gly Pro Gln Phe Leu Gln Gly Ile Leu Asn Trp Val Glu His Thr

195 200 205

Arg Lys Ala Leu Pro Ala Ser Met Thr Phe Arg Ser Phe Asn Glu Tyr

210 215 220

Ile Asp Tyr Arg Ile Gly Asp Phe Ala Val Asp Phe Cys Asp Ala Ala

225 230 235 240

Ile Leu Leu Thr Cys Glu Ile Phe Leu Thr Pro Ala Asp Met Glu Pro

245 250 255

Leu Arg Lys Leu His Arg Leu Tyr Met Thr His Phe Ser Leu Thr Asn

260 265 270

Asp Leu Tyr Ser Phe Asn Lys Glu Val Val Ala Glu Gln Glu Thr Gly

275 280 285

Ser Ala Val Ile Asn Ala Val Arg Val Leu Glu Gln Leu Val Asp Thr

290 295 300

Ser Thr Arg Ser Ala Lys Val Leu Leu Arg Ala Phe Leu Trp Asp Leu

305 310 315 320

Glu Leu Gln Ile His Asp Glu Leu Thr Arg Leu Lys Gly Thr Asp Leu

325 330 335

Thr Pro Ser Gln Trp Arg Phe Ala Arg Gly Met Val Glu Val Cys Ala

340 345 350

Gly Asn Ile Phe Tyr Ser Ala Thr Cys Leu Arg Tyr Ala Lys Pro Gly

355 360 365

Leu Arg Gly Ile

370

<210> 79

<211> 412

<212> PRT

<213> 香灰菌

<400> 79

Met Lys Ser Ser Lys Met Met Arg Thr Thr Leu Leu Arg Leu Ala Arg

1 5 10 15

Arg Ala Arg Ser Arg Leu Leu Ser Ile Leu Ser Pro His Ser Val Pro

20 25 30

Ala Ala Gln Glu Val Gln Arg Thr Ser Glu Lys Pro Ser Ala Gln Gln

35 40 45

Gly Leu Cys Gly Glu Ala Leu Val Leu Ala Ser Gln Leu Asp Gly Lys

50 55 60

Thr Phe His Val Pro Asp Leu Trp Lys Val Phe Ser Asp Trp Pro Leu

65 70 75 80

Ala Ala Asn Pro His Ala Gln Arg Leu Asp Ala Leu Val Asp Ser Leu

85 90 95

Leu Glu Arg Ile Ile Thr Asn Glu Lys Lys Leu Lys Ala Leu Lys Gln

100 105 110

Ala Asn Phe Gly Arg Leu Ile Ser Leu Trp Tyr Pro Asp Ala Glu Trp

115 120 125

Ser Glu Leu Glu Ile Ala Ala Ala Tyr Ser Val Trp Ile Phe Val Trp

130 135 140

Asp Asp Glu Val Asp Ala Asn Asp Thr Asp Val Ser Asn Asp Glu Glu

145 150 155 160

Leu Ser Arg Ala Tyr Tyr Gln Lys Ser Leu Arg Thr Ile His Asn Leu

165 170 175

Leu Gly Leu Asp Pro Val Glu Asp Gly Gln Glu Pro Val Phe Glu Asp

180 185 190

Asp Gln Ser Leu His Pro Asn Met Ala Leu Phe Ala Asp Val Gly Arg

195 200 205

Gly Met Arg Ala Thr Thr Asp Lys Ile Gln Arg Glu Arg Phe Tyr Arg

210 215 220

Glu Leu Glu Asn Phe Met Val Gln Val Gly Val Glu His Val His Arg

225 230 235 240

Met Arg Gly Ser Ile Pro Ser Val Glu Lys Tyr Ile Glu Ile Arg Ser

245 250 255

Gly Ser Val Gly Cys Ala Pro Gln Ile Ala Ile Thr Asp Ala Met Leu

260 265 270

Arg Ile Arg Leu Pro Glu Ser Ile Met Glu Cys Ala Ala Met Lys Ala

275 280 285

Leu Trp Arg Glu Thr Val Val Ile Cys Phe Ile Leu Asn Asp Val Tyr

290 295 300

Ser Val Gln Lys Glu Ile Ala Gln Asp Ser Leu Leu Asn Leu Val Pro

305 310 315 320

Val Met Tyr Lys Asn Leu Asp Pro Glu Lys Gln Ser Leu Asp Thr Val

325 330 335

Thr Arg Asp Ile Glu Val Leu Leu Gln Asp Thr Val Arg Lys Phe Glu

340 345 350

Glu Ala Ala Lys Ser Leu Ser Glu Met Thr Ser Asn Asp Ala Gln Val

355 360 365

Ser Lys Asp Val Gln Ala Phe Ile Lys Trp Cys Arg Tyr Phe Ile Thr

370 375 380

Gly Val Gln Gln Trp Ser Leu Glu Ser Arg Arg Tyr Gly Met Ala Lys

385 390 395 400

Cys Val Asn Glu Asp Gly Ser Leu Ser Ile Val Leu

405 410

<210> 80

<211> 376

<212> PRT

<213> 香灰菌

<400> 80

Met Ala Ala Thr Ile Gln Gly Asn Glu Arg Ser Gly Leu Asn Pro Gln

1 5 10 15

Leu Leu Pro Phe Ser Val Asn Thr Arg Glu Gln Leu Leu Thr Asp Thr

20 25 30

Arg Gly Ser Arg Val Met Ile Pro Asp Leu Gln Ser Met Ile Ser His

35 40 45

Trp Pro Gln Arg Thr Asn Thr Asp Val Glu Arg Leu Asp Glu Tyr Val

50 55 60

Glu Lys Ala Leu Thr Cys Phe Ser Ser Leu Ser Asn Asn Glu Ala Arg

65 70 75 80

Val Arg Arg Leu Lys Ala Thr Asn Val Ala Phe Ile Ala Ala Thr Trp

85 90 95

Trp Pro Tyr Ala Ser Tyr Lys Ala Leu Glu Val Leu Thr Ser Leu Leu

100 105 110

Leu Trp Leu Phe Ala Trp Asp Asp Glu Thr Asp Ser Pro Glu Phe Ser

115 120 125

Ala Val Ile Asn Asp Trp Asp Lys Ala Ser Thr Phe Arg Gln Arg Thr

130 135 140

Thr Asn Tyr Leu Gln Gln Ser Leu Leu Lys Asn Ser Lys Ser Asn Leu

145 150 155 160

Ala Asn Met Ser Thr Asp Pro Ile Asn Ala Leu Phe Gly Pro Val Ala

165 170 175

Glu Ala Ile Ser Glu Ser Cys Asp Asp Arg Gln Val Gly Thr Phe Leu

180 185 190

Asp Glu Leu Leu Phe Tyr Val Lys Met Cys Gly Glu Glu Gln Lys Leu

195 200 205

Gln Val Ala His Arg Leu Pro Thr Val Glu Glu Tyr Val Arg Leu Arg

210 215 220

Leu Gly Ser Gly Ala Val Arg Val Cys Phe Ala Thr Ile Glu Tyr Ala

225 230 235 240

Tyr Gly Ile Thr Leu Ser Gln Lys Ile Met Asp Asp Glu Ala Met Gln

245 250 255

Arg Ile Trp His Glu Ala Asn Ile Ile Ile His Thr Thr Asn Asp Ile

260 265 270

Leu Ser Val Lys Lys Glu Val Ala Gln Ser Gln Val Asp Ser Leu Val

275 280 285

Pro Leu Leu Ala Leu Glu Leu Gly Ser Met Gln Ala Ala Met Asn His

290 295 300

Ala Val Asp Ile Val Arg Ser Ser Ile Gln Arg Phe Asp Thr Ala Ala

305 310 315 320

Ile Glu Ile Leu Glu Arg Tyr Ala Thr Thr Pro Lys Val Gln Glu Asp

325 330 335

Ile Arg Lys Ser Ile Asp Ala Cys Arg Tyr Ala Cys Thr Ser Asn Leu

340 345 350

Asn Trp Ser Leu Val Ser Gly Arg Tyr Lys Leu Asn Cys Gln Ser Met

355 360 365

Glu Gly Gly Leu Tyr Ile Thr Leu

370 375

<210> 81

<211> 367

<212> PRT

<213> 发光类脐菇

<400> 81

Met Ala Ile Glu Asn Thr Ile Ala Ser Ala Pro Ala Ser Thr Pro Ala

1 5 10 15

Lys Gln Leu Asp Thr Pro Asp His Phe Ile Leu Pro Asp Leu Val Ser

20 25 30

His Cys Thr Phe Pro Leu Val Tyr His Ser Asn Gly Asp Ala Val Ala

35 40 45

Ala Gln Ser Val Lys Trp Leu Asp Thr Asn Cys Pro Asp Leu Asn Asp

50 55 60

Lys Arg Arg Lys Ala Leu Tyr Gly Leu Gln Ala Gly Glu Leu Thr Ala

65 70 75 80

Tyr Cys Tyr Asn Thr Ala Pro Asp Gln Arg Leu Arg Val Val Ser Asp

85 90 95

Phe Met Asn Tyr Leu Phe His Leu Asp Asn Ile Ser Asp Gly Met Met

100 105 110

Thr Lys Asp Thr Asp Ala Leu Ser Asp Ala Val Met Asn Ala Leu Trp

115 120 125

Phe Thr Glu Trp Tyr Arg Pro Thr Lys Lys Ser Asp Tyr Val Gln Pro

130 135 140

Asp Glu Glu Leu Asn Ala Gly Lys Leu Ala Arg Asp Phe Trp His Arg

145 150 155 160

Cys Ile Gln Asp Ala Gly Pro Gly Cys Gln Ala Arg Phe Lys Glu Thr

165 170 175

Leu Glu Leu Phe Phe Glu Ala Val Asn Ile Gln Ala Lys Ala Arg Asp

180 185 190

Ala Gly Val Ile Pro Asp Leu Glu Ser Tyr Ile Asp Val Arg Arg Asp

195 200 205

Thr Ser Gly Cys Lys Pro Cys Trp Ala Leu Ile Glu Tyr Gly Leu Gly

210 215 220

Ile Asp Leu Pro Asp Tyr Val Ala Glu Asp Pro Ile Ile Lys Ser Leu

225 230 235 240

Asn Gln Ser Thr Asn Asp Leu Val Thr Trp Ser Asn Asp Ile Phe Ser

245 250 255

Tyr Asn Val Glu Gln Ser Arg Gly Asp Thr His Asn Met Ile Val Ile

260 265 270

Leu Met Leu Tyr His Gly His Asn Leu Gln Ser Ala Ile Asp Tyr Val

275 280 285

Gly Asp Leu Cys Arg Gln Thr Ile Asp Asp Phe Lys Glu Asn Arg Lys

290 295 300

Lys Ile Pro Ser Trp Gly Pro Glu Val Asp Asp Ile Val Lys Gln Tyr

305 310 315 320

Val Gln Gly Leu Gln Asp Trp Ile Val Gly Ser Leu His Trp Ser Phe

325 330 335

Met Thr Thr Arg Tyr Phe Gly Lys Gln Gly Gln Glu Val Lys Lys Asn

340 345 350

Arg Tyr Val Lys Leu Leu Pro Val Gly Glu Glu Ala Asn Lys Trp

355 360 365

<210> 82

<211> 425

<212> PRT

<213> 灰盖鬼伞

<400> 82

Met Ser Thr Pro Ser Ser Ser Leu Thr Thr Asp Glu Ser Pro Ala Ser

1 5 10 15

Phe Ile Leu Pro Asp Leu Val Ser His Cys Pro Phe Pro Leu Arg Tyr

20 25 30

His Pro Lys Gly Asp Glu Val Ala Lys Gln Thr Val His Trp Leu Asp

35 40 45

Ser Asn Cys Pro Asp Leu Thr Ala Lys Glu Arg Lys Ala Met Tyr Gly

50 55 60

Leu Gln Ala Gly Glu Leu Thr Gly Tyr Cys Tyr Pro Tyr Thr Thr Pro

65 70 75 80

Glu Arg Leu Arg Val Val Ala Asp Phe Leu Asn Tyr Leu Phe His Leu

85 90 95

Asp Asn Ile Ser Asp Gly Met Met Thr Arg Glu Thr Ala Val Leu Ala

100 105 110

Asp Val Val Met Asn Ala Leu Trp Phe Pro Glu Asp Tyr Arg Pro Thr

115 120 125

Lys Gly Gln Ala Ala Glu Glu Leu Asn Pro Gly Lys Leu Ala Arg Asp

130 135 140

Phe Trp Ser Arg Cys Ile Pro Asp Cys Gly Pro Gly Thr Gln Ala Arg

145 150 155 160

Phe Lys Glu Thr Phe Gly Ser Phe Phe Glu Ala Val Asn Ile Gln Ala

165 170 175

Arg Ala Arg Asp Glu Gly Val Ile Pro Asp Leu Glu Ser Tyr Ile Asp

180 185 190

Val Arg Arg Asp Thr Ser Gly Cys Lys Pro Cys Trp Val Leu Ile Glu

195 200 205

Tyr Ala Leu Gly Ile Asp Leu Pro Asp Phe Val Val Glu His Pro Val

210 215 220

Ile Ala Ala Leu Asn Gln Gly Thr Asn Asp Leu Val Thr Trp Ser Asn

225 230 235 240

Asp Ile Phe Ser Tyr Asn Val Glu Gln Ser Lys Gly Asp Thr His Asn

245 250 255

Met Ile Ile Ile Leu Met Glu His His Gly His Thr Leu Gln Ser Ala

260 265 270

Val Asp Tyr Val Gly Ser Leu Cys Gln Gln Thr Ile Asn Thr Phe Cys

275 280 285

Glu Asn Lys Gln Gln Leu Pro Ser Trp Gly Pro Glu Ile Asp Asp Met

290 295 300

Val Ala Lys Tyr Val Gln Gly Leu Glu Asp Trp Ile Val Gly Ser Leu

305 310 315 320

His Trp Ser Phe Gln Thr Arg Arg Tyr Phe Gly Asp Glu Gly Gln Glu

325 330 335

Ile Lys Gln His Arg Leu Val Lys Leu Leu Thr Val Ala Pro Pro Pro

340 345 350

Pro Pro Pro Pro Pro Thr Pro Pro Pro Gln Ser Ser Asp Ala Asp Thr

355 360 365

Lys Lys Gln Lys Val Lys Ala Gln Asp Gly Lys Gly Pro Val Ser Asp

370 375 380

Glu Glu Val Trp Ala Leu Val Arg Ala Glu Gln Ser Lys Gly Ser Ile

385 390 395 400

Leu Glu Ser Leu Phe Gly Phe Leu Thr Thr Ser Leu Ser Arg Ile Phe

405 410 415

Phe Gly Tyr Phe Phe Ala Tyr Ser His

420 425

<210> 83

<211> 345

<212> PRT

<213> 灰盖鬼伞

<400> 83

Met Ser Ser Leu Asp Ala Thr Ile His Pro Val Leu Asn Phe Glu Asp

1 5 10 15

Lys Lys Ile Val Leu Pro Asp Leu Val Ser His Cys Asn Phe Lys Leu

20 25 30

Arg Val Ser Arg His Arg Lys Arg Ile Thr Gly Glu Thr Lys Arg Trp

35 40 45

Leu Phe Lys Gly Asp Asn Leu Val Gly Pro Ala Arg Asn Lys Tyr His

50 55 60

Gly Leu Lys Ala Gly Leu Leu Thr Ala Met Thr Tyr Pro Asp Ala Ala

65 70 75 80

Tyr Pro Gln Leu Arg Leu Cys Asn Asp Phe Leu Thr Tyr Leu Phe His

85 90 95

Ile Asp Asn Leu Ser Asp Asp Met Asp Asn Arg Gly Thr Trp Ser Thr

100 105 110

Ala Asn Glu Val Leu Asn Ser Leu Tyr His Pro Tyr Thr Tyr His Gly

115 120 125

Gln Ala Arg Val Gly Arg Met Thr Arg Asp Tyr Trp Arg Arg Met Ile

130 135 140

Leu Thr Ala Ser Pro Gly Ser Gln Gln Arg Phe Ile Glu Thr Phe Asp

145 150 155 160

Phe Phe Phe Gln Ser Val Thr Gln Gln Ala Ile Asp Arg Leu Thr Gly

165 170 175

Glu Ile Pro Asp Leu Glu Ser Tyr Ile Ala Leu Arg Arg Asp Thr Ser

180 185 190

Gly Cys Lys Pro Cys Trp Ala Leu Ile Glu Tyr Ala Asn Asn Leu Asp

195 200 205

Leu Pro Asp Glu Val Met Asp His Pro Val Val Arg Ser Leu Gly Glu

210 215 220

Ala Ala Asn Asp Leu Val Thr Trp Ser Asn Asp Ile Phe Ser Phe Asn

225 230 235 240

Val Glu Gln Ser Lys Gly Asp Thr His Asn Met Ile Pro Val Val Met

245 250 255

His Gln Glu Gly Leu Asp Leu Gln Ser Ala Val Asp Phe Val Gly Glu

260 265 270

Met Cys Lys Ser Ala Ile Asp Arg Phe Ile Glu Asp Gln Asn Tyr Leu

275 280 285

Pro Ser Trp Gly Pro Lys Ile Asp Arg Asp Leu Ala Val Tyr Ile Asn

290 295 300

Gly Leu Ala Asp Trp Ile Val Gly Ser Leu His Trp Ser Phe Glu Thr

305 310 315 320

Glu Arg Tyr Phe Gly Lys Asn Gly Arg Gln Val Lys Ser Ser Arg Val

325 330 335

Ile Asp Leu Leu Pro Arg Arg Ser Gln

340 345

<210> 84

<211> 332

<212> PRT

<213> 发光类脐菇

<400> 84

Met Lys Tyr Thr Ser Phe Ala Leu Pro Asp Leu Ala Ser Ser Cys Asp

1 5 10 15

Tyr Asn Leu Arg Phe Asn Lys Tyr His Arg Ser Val Ser Pro Glu Thr

20 25 30

Lys Lys Trp Phe Phe Arg Leu Ser Pro Ala Ser Gln Ala Asp Leu Thr

35 40 45

Thr Tyr Asp Ala Gln Arg Phe Thr Leu Leu Ala Ala Val Cys Tyr Pro

50 55 60

Asp Ala Gly Tyr Pro Gln Leu Arg Val Cys Ser Asp Phe Leu Ala Tyr

65 70 75 80

Leu Phe Tyr Leu Asp Asn Leu Thr Asp Asp Met Asp Asp Lys Ser Thr

85 90 95

Arg Ser Val Ala Asp Leu Val Leu Asn Ser Leu Asn Glu Pro Glu Thr

100 105 110

Phe Gln Thr Gln Tyr Arg Ile Gly Lys Met Thr Ser Asp Tyr Phe Lys

115 120 125

Arg Ile Ile Gln Thr Ser Asn Asp Gly Thr Lys Lys Arg Phe Ile Asp

130 135 140

Thr Met Asp Ser Phe Phe Lys Ser Val Asp Asp Gln Ala Arg Asp Arg

145 150 155 160

Leu Ala Gly His Ile Pro Asp Leu Glu Ser Tyr Ile Ala Leu Arg Arg

165 170 175

Glu Thr Ser Gly Cys Lys Thr Cys Phe Ser Leu Ile Glu Tyr Ala Asn

180 185 190

Asn Leu His Ile Pro Asp Glu Val Ile Ser His Pro His Ile Glu Gln

195 200 205

Met Glu Thr Ala Ala Asn Asp Val Val Ser Phe Ala Asn Asp Ile Tyr

210 215 220

Ser Phe Asn Ile Glu Gln Ser Lys Gly Asp Thr His Asn Met Ile Pro

225 230 235 240

Val Leu Met His Ala Asn Pro Asp Met Asp Phe Leu Glu Ala Val Ser

245 250 255

Phe Val Arg Asp Leu Thr Ile Lys Ala Met Asp Arg Phe Asn Glu Leu

260 265 270

Arg Ala Thr Leu Pro Ser Trp Gly Leu Asp Ile Asp Lys Asp Met Lys

275 280 285

Val Tyr Val Asn Gly Leu Glu Asn Trp Met Val Gly Ile Leu Phe Trp

290 295 300

Ser Phe Glu Thr Glu Arg Tyr Phe Gly Lys Ser Val Arg Ser Val Lys

305 310 315 320

Ala Thr Lys Thr Val Asn Leu Leu Pro Ser Arg Ala

325 330

<210> 85

<211> 333

<212> PRT

<213> 发光类脐菇

<400> 85

Met Ala Ser Thr Ala Pro Ser Lys Phe Ile Leu Pro Asp Leu Val Ser

1 5 10 15

His Cys Ser Phe Asp Leu His His Asn Arg His Arg Lys Gln Ile Thr

20 25 30

Thr Glu Thr Lys Lys Trp Leu Phe Lys Gly Asp Asn Leu Thr Gly Arg

35 40 45

Lys Arg Asp Gln Tyr His Gly Leu Lys Cys Gly Leu Leu Ser Ala Met

50 55 60

Cys Tyr Pro Asn Ala Ala Tyr Pro Gln Leu Arg Val Cys Asn Asp Phe

65 70 75 80

Leu Thr Tyr Leu Phe His Leu Asp Asn Leu Ser Asp Asp Met Asp Asn

85 90 95

Arg Gly Thr Thr Thr Thr Ala Asp Val Val Leu Asn Ser Leu Tyr His

100 105 110

Pro Gly Tyr Phe Gln Ser Ala Arg Val Gly Lys Met Thr Arg Asp Tyr

115 120 125

Trp Lys Arg Leu Ile Ser Thr Ala Ser Pro Gly Thr Gln Gln Arg Phe

130 135 140

Ile Glu Thr Phe Asp Phe Phe Phe Gln Ser Val Thr Glu Gln Ala His

145 150 155 160

Asp Arg Gln Ala Gly Val Ile Pro Asp Leu Glu Ser Tyr Ile Ala Leu

165 170 175

Arg Arg Asp Thr Ser Gly Cys Lys Thr Ser Phe Val Leu Ile Glu Tyr

180 185 190

Ala Asn Asn Leu Asp Ile Pro Asp Gly Val Met Asp His Pro Leu Ile

195 200 205

Arg Ser Leu Gly Glu Ala Ala Asn Asp Leu Val Thr Trp Ser Asn Asp

210 215 220

Ile Phe Ser Tyr Asn Val Glu Gln Ala Lys Gly Asp Thr His Asn Met

225 230 235 240

Ile Pro Val Ile Met Asn Glu His Gly Leu Asp Leu Gln Ser Ala Val

245 250 255

Asp Tyr Val Gly Arg Leu Cys Gln Gln Ser Ile Asp Arg Phe Ile Ser

260 265 270

Asp Arg Ala Gln Leu Pro Ser Trp Gly Pro Glu Ile Asp Arg Gln Val

275 280 285

Ala Ile Tyr Val Asp Gly Leu Thr Asp Trp Ile Val Gly Ser Leu His

290 295 300

Trp Ser Phe Glu Ser Glu Arg Tyr Phe Gly Lys Ser Gly Arg Gln Ile

305 310 315 320

Lys Lys Ser Arg Val Ile Asn Leu Leu Pro Arg Arg Ala

325 330

<210> 86

<211> 348

<212> PRT

<213> 灰盖鬼伞

<400> 86

Met Pro Ser Pro Ala Gly Ala Leu Pro Lys Ser Phe Ile Leu Pro Asp

1 5 10 15

Leu Val Asn Asp Cys Pro Phe Pro Leu Arg Val Asn Pro Leu Cys Asp

20 25 30

Glu Val Gly Arg Leu Ser Glu Gln Trp Phe Leu Arg His Ala Asn Tyr

35 40 45

Ser Pro Pro Arg Ala Val Ala Phe Met Ala Leu Lys Ala Gly Glu Leu

50 55 60

Thr Ala Ala Cys Tyr Pro Asp Ala Asp Ala Phe His Leu Arg Val Ser

65 70 75 80

Asp Asp Phe Met Asn Phe Leu Phe Asn Ala Asp Asp Trp Leu Asp Asp

85 90 95

Phe Asp Ile Glu Asp Thr Tyr Gly Leu Ala Asn Cys Thr Val Arg Ala

100 105 110

Leu Arg Asp Pro Val Asn Phe Ile Thr Asp Lys Arg Ala Gly Leu Met

115 120 125

Thr Lys Ser Tyr Phe Ser Arg Phe Leu Lys Thr Ala Gly Pro Arg Cys

130 135 140

Thr Glu Arg Phe Ile Gln Thr Leu Ala Leu Tyr Phe Glu Ser Val Val

145 150 155 160

Thr Gln Lys Gln Ala Arg Asn Asn Gly Thr Leu Pro Asp Leu Glu Ser

165 170 175

Tyr Ile Thr Ile Arg Arg Asn Asn Ser Gly Cys Lys Pro Cys Tyr Ala

180 185 190

Leu Ile Glu Phe Cys Ala Gly Ile Asp Leu Pro Asp Glu Val Ile Asn

195 200 205

His Pro Ile Ile Gln Ser Leu Glu Asp Ala Ser Asn Asp Leu Ile Ala

210 215 220

Trp Ser Asn Asp Ile Phe Ser Phe Asn Arg Glu Gln Ser Arg His Asp

225 230 235 240

Ser Phe Asn Met Val Ser Ile Val Met His Gln Lys Gly Phe Ala Leu

245 250 255

Gln Glu Ala Val Asn Phe Val Gly Glu Leu Cys Lys Lys Ala Met Glu

260 265 270

Arg Phe Gln Ala Asp Lys Arg Asn Leu Pro Ser Trp Gly Pro Glu Ile

275 280 285

Asp Gly Glu Val Ala Met Tyr Val Asp Gly Leu Gln Asn Trp Ile Val

290 295 300

Gly Ser Leu Asn Trp Ser Ile Asp Gly Thr Glu Arg Tyr Phe Gly Lys

305 310 315 320

Asp Gly Pro Gly Ile Lys Lys His Arg Lys Val Lys Leu Phe Pro Lys

325 330 335

Arg Pro Leu Lys Thr Pro Ala Val Arg Val Leu Ala

340 345

<210> 87

<211> 345

<212> PRT

<213> 灰盖鬼伞

<400> 87

Met Arg Pro Thr Ala Arg Gln Phe Thr Leu Pro Asp Leu Phe Ser Ile

1 5 10 15

Cys Pro Leu Gln Asp Ala Thr Asn Pro Trp Tyr Lys Gln Ala Ala Ala

20 25 30

Glu Ser Arg Ala Trp Ile Asn Ser Tyr Asn Ile Phe Thr Asp Arg Lys

35 40 45

Arg Ala Phe Phe Ile Gln Gly Ser Asn Glu Leu Leu Cys Ser His Val

50 55 60

Tyr Ala Tyr Ala Gly Tyr Glu Gln Phe Arg Thr Cys Cys Asp Phe Val

65 70 75 80

Asn Leu Leu Phe Val Val Asp Glu Ile Ser Asp Asp Gln Asn Gly Gln

85 90 95

Asp Ala Arg Ala Thr Gly Arg Ile Phe Val Asn Ala Met Arg Asp Ala

100 105 110

His Trp Asp Asp Gly Ser Ile Leu Ala Lys Ile Thr His Glu Phe Arg

115 120 125

Glu Arg Phe Val Arg Leu Ala Gly Pro Lys Thr Val Arg Arg Phe Ala

130 135 140

Asp Leu Cys Glu Ser Tyr Thr Asp Cys Val Ala Arg Glu Ala Glu Leu

145 150 155 160

Arg Glu Arg Asn Gln Val Leu Gly Leu Asn Asp Phe Ile Ala Leu Arg

165 170 175

Arg Gln Asn Ser Ala Val Leu Leu Cys Tyr Ser Leu Val Glu Tyr Ile

180 185 190

Leu Gly Ile Asp Leu Asp Asp Glu Val Tyr Glu Asp Pro Thr Phe Ala

195 200 205

Lys Ala Tyr Trp Ala Ala Cys Asp Phe Val Cys Trp Ala Asn Asp Val

210 215 220

Tyr Ser Tyr Asp Met Glu Gln Ala Lys Gly His Thr Gly Asn Asn Val

225 230 235 240

Val Thr Val Leu Met Lys Glu Lys Asp Leu Ser Leu Gln Glu Ala Ser

245 250 255

Asp Tyr Ile Gly Arg Glu Cys Glu Lys Gln Met Arg Asp Tyr Leu Glu

260 265 270

Ala Lys Ser Gln Leu Leu Gln Ser Thr Asp Leu Pro Gln Glu Ala Val

275 280 285

Arg Tyr Ile Glu Ala Leu Gly Tyr Trp Met Val Gly Asn Leu Val Trp

290 295 300

Ser Phe Glu Ser Gln Arg Tyr Phe Gly Ala Gln His Glu Arg Val Lys

305 310 315 320

Ala Thr His Val Val His Leu Arg Pro Ser Ser Val Leu Glu Ala Ser

325 330 335

Cys Asp Ser Asp Ser Asp Ser Asp Cys

340 345

<210> 88

<211> 346

<212> PRT

<213> 毛韧革菌

<400> 88

Met Ala Thr Ser Asn Pro Pro Ser Thr Pro His His Glu Ser Leu Ile

1 5 10 15

Leu Pro Asp Leu Leu Ser Leu Ser Thr Pro Phe Asn Gly Ser Thr Asn

20 25 30

Pro His Trp Ala Met Ala Ala Pro Glu Ser Ser Ala Trp Val Ser Ser

35 40 45

Tyr Asn Leu Phe Ser Asp Arg Lys Arg Thr Asp Phe Ile Thr Gly Ser

50 55 60

Asn Glu Leu Leu Val Ser His Thr Tyr Pro His Ala Asp Tyr Asp Ala

65 70 75 80

Phe Arg Thr Cys Cys Asp Phe Val Asn Leu Leu Phe Val Ile Asp Glu

85 90 95

Ile Ser Asp Asp Gln Ser Gly Lys Ala Ala Arg Arg Thr Gly Glu Val

100 105 110

Tyr Leu Asn Ala Met Arg Asp Pro Glu Trp Thr Asp Gly Ser Asp Leu

115 120 125

Ala Lys Met Thr Gln Gln Phe Arg Ala Arg Phe Leu Arg Ser Val Gly

130 135 140

Pro Gln Ser Phe Arg Arg Phe Leu Arg His Ser Glu Asp Tyr Ile Asp

145 150 155 160

Cys Val Ala Lys Glu Ala Glu Tyr Arg Glu Arg Gly Gln Val Leu Asp

165 170 175

Met Asp Ser Phe Lys Ser Leu Arg Arg Glu Asn Ser Ala Ile Arg Leu

180 185 190

Cys Phe Gly Leu Phe Glu Phe Thr Leu Gly Ile Asp Leu Pro Asp Ser

195 200 205

Val Phe Glu Asp Glu Thr Phe Met Lys Met Tyr Trp Ala Ser Ala Asp

210 215 220

Met Val Cys Trp Ala Asn Asp Val Tyr Ser Tyr Asn Val Glu Gln Ala

225 230 235 240

Lys Gly His Ser Gly Asn Asn Ile Val Thr Val Leu Met Ala Ala Arg

245 250 255

Asp Ile Asp Met Gln Ala Ala Ser Asp Tyr Val Gly Glu Tyr Tyr Ala

260 265 270

Glu Leu Met Glu Glu Tyr Met Thr Ala Lys Ala Glu Leu Ala Ser Lys

275 280 285

Ser Phe Gly Ser Arg Asp Leu Asp Glu Asp Val Trp Lys Tyr Val Asn

290 295 300

Ala Met Glu Asn Trp Pro Ile Gly Asn Leu Glu Trp Ser Phe Lys Thr

305 310 315 320

Asn Arg Tyr Phe Gly Thr Leu His Asp Glu Val Lys Arg Thr Arg Leu

325 330 335

Val Val Ile Lys Pro Arg Lys Val Val Val

340 345

<210> 89

<211> 344

<212> PRT

<213> 发光类脐菇

<400> 89

Met Ser Ser Ala Pro Thr Arg Phe Leu Leu Pro Asp Leu Leu Ser Ala

1 5 10 15

Cys Pro Leu Lys Gly Ser Val Asn Pro Tyr Tyr Lys Glu Ala Gly Ala

20 25 30

Glu Ser Ser Ala Trp Ile Asn Ser Tyr Asp Ile Phe Thr Asp Arg Lys

35 40 45

Arg Ala Phe Phe Val Gln Gly Cys Asn Glu Leu Leu Val Ala His Thr

50 55 60

Tyr Pro Tyr Ala Gly Tyr Glu Glu Phe Arg Thr Cys Cys Asp Phe Ile

65 70 75 80

Asn Val Leu Phe Val Leu Asp Glu Val Ser Asp Glu Gln Ser Gly Ser

85 90 95

Asp Ala Arg Phe Thr Gly Glu Val Phe Leu Asn Ala Leu Arg Asn Pro

100 105 110

Glu Asn Asp Asp Thr Ser Lys Leu Ser Lys Ile Ser Lys Glu Phe Arg

115 120 125

Ala Arg Tyr Phe Lys Arg Ala Gly Pro Arg Thr Ala Glu Arg Phe Leu

130 135 140

Gln His Cys Gln Asp Tyr Ile Asp Cys Val Thr Arg Glu Ala Glu Leu

145 150 155 160

Arg Glu Arg Gly Glu Val Leu Asp Leu Pro Ser Phe Thr Ala Leu Arg

165 170 175

Arg Glu Asn Ser Ala Ile Arg Val Cys Phe Cys Leu Phe Glu Tyr Ala

180 185 190

Leu Gly Phe Asp Leu Pro Gln Glu Val Phe Asp Asp Pro Thr Phe Met

195 200 205

Glu Met Tyr Trp Ala Ala Ala Asp Leu Val Cys Trp Ala Asn Asp Val

210 215 220

Tyr Ser Tyr Asn Lys Glu Gln Ala Gln Gly His Gly Gly Asn Asn Ile

225 230 235 240

Val Thr Val Leu Met Lys Ala Lys Asp Leu Asp Leu Gln Ala Ala Cys

245 250 255

Asp Tyr Ile Gly Val Tyr Cys Glu Glu Leu Met Gly Arg Tyr Leu Ser

260 265 270

Ala Lys Ala Arg Leu Pro Ser Trp Gly Pro Glu Val Asp Ala Ala Val

275 280 285

Ala Gln Tyr Val Glu Ala Ser Gly His Trp Val Arg Gly Asn Leu Asp

290 295 300

Trp Ser Phe Glu Thr Gln Arg Tyr Phe Gly Ala Gln His Ala Glu Ile

305 310 315 320

Lys Glu Thr Arg Leu Val Thr Leu Thr Pro Ala Ile Pro Glu Asp Phe

325 330 335

Ser Asp Thr Gly Ser Glu Ser Glu

340

<210> 90

<211> 345

<212> PRT

<213> 发光类脐菇

<400> 90

Met Ser Pro Asp Pro Thr Arg Ile Val Leu Pro Asp Phe Leu Ala Ala

1 5 10 15

Cys Pro Phe Glu Ser Ser Thr Lys Asn Pro His Leu Lys Ala Ala Gly

20 25 30

Ala Glu Ser Ser Ala Trp Val Asn Ser His Val Val Phe Asn Asp Arg

35 40 45

Lys Arg Ala Ala Phe Met Gln Asp Ile Tyr Glu Leu Leu Val Ala Tyr

50 55 60

Ala Phe Pro Trp Ala Asp Tyr Glu Asp Phe Arg Thr Met Cys Asp Phe

65 70 75 80

Ile Asn Leu Leu Phe Val Leu Asp Glu Leu Ser Asp Asp Gln Asn Gly

85 90 95

Lys Asp Ala Gly Tyr Thr Gly Lys Leu Phe Met Asp Ala Met Arg Asn

100 105 110

Ile Asp Asn Gly Asp Thr Ser Glu Leu Thr Glu Leu Cys Arg Glu Phe

115 120 125

Lys Ala Arg Tyr Ser Lys Arg Val Ser Pro Gln Val Asn Glu Arg Phe

130 135 140

Leu Gln His Leu Gln Ser Tyr Thr Asp Cys Val Ala Gln Glu Ala Asp

145 150 155 160

Leu Arg Glu Arg Gly Glu Ile Leu Asp Leu Glu Ser Tyr Val Ala Leu

165 170 175

Arg Arg Glu Asn Ser Ala Ile Arg Pro Cys Phe Asp Leu Val Glu Tyr

180 185 190

Ile Ile Asp Phe Asp Ile Pro Gln Glu Val Ile Asp His Pro Val Phe

195 200 205

Ser Glu Met Tyr Trp Ala Ser Val Asp Leu Val Cys Trp Ser Asn Asp

210 215 220

Val Tyr Ser Tyr Asn Val Glu Gln Ala Lys Gly His Gly Gly Ser Asn

225 230 235 240

Val Val Thr Val Leu Met Lys Glu Lys Asn Leu Asp Leu Gln Ala Ala

245 250 255

Cys Asp Tyr Val Gly Val Tyr Tyr Glu Glu Leu Met Asp Arg Tyr Leu

260 265 270

Ser Ala Lys Ala Arg Leu Pro Ser Trp Gly Pro Glu Ile Asp Ala Ala

275 280 285

Val Gly Lys Tyr Ile Leu Ala Glu Ala Gln Phe Val Arg Gly Asn Leu

290 295 300

Asp Trp Ser Phe Asp Ser Pro Arg Tyr Phe Gly Pro Gln His Asp Gln

305 310 315 320

Val Lys Lys Thr Gly Ile Val Thr Leu Thr Pro Ala Pro Lys Lys Phe

325 330 335

Gly Ser Asp Ser Gly Ser Glu Ser Glu

340 345

<210> 91

<211> 345

<212> PRT

<213> 发光类脐菇

<400> 91

Met Ser Pro Ala Pro Ser Arg Ile Val Leu Pro Asp Phe Phe Ala Ser

1 5 10 15

Cys Pro Phe Glu Ser Ser Thr Ile Asn Pro His Phe Lys Ala Ala Gly

20 25 30

Ala Glu Ser Ser Ala Trp Val Asn Ser His Val Val Phe Asn Asp Arg

35 40 45

Lys Arg Ala Ala Leu Met Gln Asn Ser Tyr Glu Leu Leu Val Ala Tyr

50 55 60

Ala Phe Pro Trp Ala Ser Tyr Glu Asp Phe Arg Thr Leu Cys Asp Phe

65 70 75 80

Ile Asn Leu Leu Phe Val Phe Asp Glu Val Ser Asp Asp Gln Asn Gly

85 90 95

Lys Asp Ala Gly Tyr Thr Ser Lys Ile Phe Met Asp Ala Met Arg Asn

100 105 110

Ile Asp Asn Gly Asp His Ser Glu Leu Thr Glu Leu Cys Lys Glu Phe

115 120 125

Lys Ala Arg Phe Ser Arg Arg Leu Ser Pro Gln Val Asn Glu Arg Phe

130 135 140

Leu Gln His Leu Gln Ser Tyr Thr Asp Cys Val Ala Gln Glu Ala Asp

145 150 155 160

Leu Arg Glu Arg Gly Glu Ile Leu Asp Leu Glu Ser Tyr Val Ile Leu

165 170 175

Arg Arg Glu Asn Ser Ala Val Arg Pro Cys Phe Asp Leu Val Glu Tyr

180 185 190

Ile Met Asp Phe Asp Ile Pro Gln Glu Val Leu Asp His Pro Val Phe

195 200 205

Ser Glu Met Tyr Trp Ala Ser Val Asp Leu Val Cys Trp Ser Asn Asp

210 215 220

Val Tyr Ser Tyr Asn Val Glu Gln Ala Lys Gly His Arg Gly Ser Asn

225 230 235 240

Val Val Thr Val Leu Met Asn Glu Lys Asn Leu Asp Leu Gln Ala Ala

245 250 255

Cys Asp Tyr Val Gly Val Tyr Tyr Gln Glu Leu Met Asp Arg Tyr Leu

260 265 270

Ser Ala Lys Ala Arg Leu Pro Ser Trp Gly Pro Glu Ile Asp Ala Ala

275 280 285

Val Gly Lys Tyr Val Leu Ala Glu Ala Gln Phe Val Arg Gly Asn Leu

290 295 300

Asp Trp Ser Phe Asp Thr Pro Arg Tyr Phe Gly Pro Gln Arg Asp Gln

305 310 315 320

Ile Lys Lys Ser Arg Ile Val Thr Leu Thr Pro Ala Pro Lys Lys Phe

325 330 335

Gly Ser Asp Ser Gly Ser Glu Ser Glu

340 345

<210> 92

<211> 355

<212> PRT

<213> 柱状田头菇

<400> 92

Met Gln Ile Ile Leu Pro Asp Ile Leu Gln Thr Trp Ala Tyr Ala Arg

1 5 10 15

Leu Leu Asn Pro His Tyr Asp Gly Ala Lys Leu Glu Ser Ser Leu Trp

20 25 30

Ile His Pro Leu Val Ala Lys Leu Phe Asp Gln Lys Gly Gln Lys Ala

35 40 45

Phe Gln Asn Asp Tyr Thr Ser Leu Leu Ala Ser Leu Met Tyr Ser His

50 55 60

Gln Gly Lys Val Pro Ser Arg Arg Cys Asp Met Met Asn Leu Phe Phe

65 70 75 80

Val Tyr Asp Glu Tyr Thr Asp Val Val Ser Pro Glu Ile Ala His Arg

85 90 95

Leu Ser Lys Ile Val Val Asp Ala Met Lys Asn Ser Asp Glu Met Ser

100 105 110

Pro Cys Gly Glu His Pro Ile Gly Asp Lys Ala Lys Glu Phe Trp Arg

115 120 125

Leu Ala Thr Thr Leu Leu Pro Ala Thr Gly Ser Asn Ser Asp Val Cys

130 135 140

Lys Ser Arg Phe Ile Asn Leu Thr Glu Glu Tyr Leu Asn Ala Val Thr

145 150 155 160

Val Glu Ala Arg Asp Arg Asn Glu Gly Thr Ile His Ser Val Lys Glu

165 170 175

Tyr Leu Thr Met Arg Arg Ala Thr Ser Gly Ala Gly Leu Met Leu Ala

180 185 190

Leu Ile Glu Phe Glu Leu Asp Leu Pro Lys Ala Val Leu Glu His Lys

195 200 205

Phe Val Gln Ala Leu Glu Glu Ile Tyr Thr Arg Thr Arg Val Ser Ser

210 215 220

Gly Gln Ala Asn His Asn Leu Ile Thr Val Val Met His Glu Asn Pro

225 230 235 240

Gly Leu Ser Leu Gln Gly Ala Phe Asp Trp Leu Gly Ser Tyr Ala Ala

245 250 255

Gly Val Val Glu Cys Phe Gln Thr Asn Val Arg Asn Leu Pro Ser Phe

260 265 270

Cys Asp Val Glu Gly Pro Ala Cys Glu Ser Val Asp Gly Thr Leu Gln

275 280 285

Glu Arg Val Asp Lys Tyr Ile Ser Gly Leu Gly Gln Ala Val Arg Ala

290 295 300

Glu Asp Asp Trp Ala Phe Glu Thr Thr Arg Tyr Tyr Gly Glu Asp Gly

305 310 315 320

Pro Lys Val Arg Glu Thr Arg Val Leu Val Ile Arg Pro Val Lys Arg

325 330 335

Ile Thr Arg Arg His Leu Leu Gln Ser Leu Glu Ile Lys Tyr Ser Met

340 345 350

Val Arg Gly

355

<210> 93

<211> 307

<212> PRT

<213> 柱状田头菇

<400> 93

Met Ser Leu Asn Phe Phe Leu Arg Arg Tyr Thr Val Phe Pro Trp Ser

1 5 10 15

Arg Lys Leu Gly Gln Tyr Tyr His Asp Ala Lys Arg Glu Ser Ser Ala

20 25 30

Trp Thr Glu Ser Phe His Pro Phe Asp Glu Asp Leu Ser Lys Ala Ala

35 40 45

Leu Thr Phe Cys Ile Thr Ala Leu Leu Ala Ser Leu Ala Tyr Phe Leu

50 55 60

Arg Gln Lys Glu Ile Val Arg Leu Gly Cys Asp Leu Met Asn Ile Phe

65 70 75 80

Tyr Val Phe Asp Glu Cys Ala Asp Ile Ala Asp Lys Glu Gly Ala Ser

85 90 95

Gln Ile Arg Asp Val Val Met Asp Asp Leu His Arg Pro Glu Lys Thr

100 105 110

Cys Pro Gly Gly Glu Ile Leu Pro Gly Glu Met Val Lys Tyr Val Leu

115 120 125

Leu Leu Cys Pro Glu Ile Pro Glu Thr Tyr Tyr Asn Thr Lys Ser Phe

130 135 140

Gly Phe Ala Pro Gln Ser Ser Ser Pro Gln Pro His Ile Val Cys Ala

145 150 155 160

Thr Leu Ser Arg Ile Ser Met Pro Thr Gln Gln Gln Trp Phe Asn Val

165 170 175

Lys Arg Thr Ile Gly Pro Asn Val Phe Leu Ala Arg Ser Ala Thr Val

180 185 190

Leu Pro Tyr Ala Glu Thr His Thr Phe Tyr His Pro Arg Met Ile Ala

195 200 205

Leu Arg Glu Gln Ala Pro Phe Leu Asp Ile Asn Ser Tyr Pro Met Lys

210 215 220

Val Arg Gly Leu Val Gln Gly Ser Ile Asn Trp Leu Glu Gly Tyr Ala

225 230 235 240

Ala Gly Val Gln Ala Ala Phe Leu Asp Asn Ile Ala Asn Leu Pro Ser

245 250 255

Cys Ala Lys Glu Val Glu Ser Arg Val Asn Ile Tyr Val Asn Glu Leu

260 265 270

Ala Gln Trp Ala Arg Gly Asn Asp Asp Trp Thr Phe Glu Ser Gly Arg

275 280 285

Tyr Phe Gly Asp Arg Gly Pro Glu Asn Gln Ser Asp Ile Pro Thr Ser

290 295 300

Ser Asn Arg

305

<210> 94

<211> 345

<212> PRT

<213> 柱状田头菇

<400> 94

Met Pro Gly Ser Ala Asn Trp Thr Ala Asp Arg Phe Tyr Ile Pro Asp

1 5 10 15

Thr Leu Ala Asn Trp Pro Trp Pro Arg Ala Ile Asn Pro Ala Tyr Glu

20 25 30

Glu Cys Lys Ala Ala Ser Ala Ala Trp Cys Glu Lys Tyr Gly Ala Phe

35 40 45

Ser Ala Arg Ala Gln Lys Ala Phe Asn Leu Cys Asp Phe Asn Leu Leu

50 55 60

Ala Ser Leu Ala Tyr Ala Gly Leu Pro Ala Asp Val Asn Arg Val Gly

65 70 75 80

Cys Asp Leu Met Asn Leu Phe Phe Val Val Asp Glu His Thr Asp Ala

85 90 95

Met Asp Ala Arg Ser Val Gln Asp Trp Val Asp Ile Val Val Asp Ala

100 105 110

Leu His His Pro His Thr Pro Arg Pro Ala Gly Glu Pro Lys Val Gly

115 120 125

Glu Ile Ala Arg Thr Phe Trp Glu Asn Gly Ile Lys Cys Met Gly Pro

130 135 140

Thr Ala Gln Arg Arg Phe Val Glu Thr Phe Thr Thr Tyr Leu Gln Ser

145 150 155 160

Val Val Thr Gln Ala Gln Asp Arg Asp Lys His Leu Phe Arg Asp Val

165 170 175

Asp Ser Tyr Met Glu Val Arg Arg Asp Thr Ile Gly Ala Lys Pro Ser

180 185 190

Phe Ala Leu Leu Glu His Asp Met Glu Leu Pro Asp Asp Val Phe Tyr

195 200 205

His Pro Leu Leu Glu Lys Leu Arg Glu Trp Ala Ile Asp Met Leu Ile

210 215 220

Leu Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ser Arg Gly Asp

225 230 235 240

Asp Gly His Asn Ile Ile Arg Leu Ala Met Leu Gln Glu Asn Thr Asn

245 250 255

Val His Gly Ala Leu Arg Phe Val Ser Lys Met His Asp Asp Leu Ala

260 265 270

Glu Lys Phe Leu Ser Asn Tyr Gln Gly Met Pro Ser Phe Thr Pro Gln

275 280 285

Ile Asp Ala Trp Val Thr Arg Tyr Ile Asp Gly Leu Gly Asn Trp Val

290 295 300

Arg Ala Asn Asp Ser Trp Ser Phe Glu Ser Trp Arg Tyr Phe Lys Gly

305 310 315 320

Asp Val Leu Arg Val Gln Ala Glu Arg Trp Val Glu Leu Leu Pro Pro

325 330 335

Ala Pro Lys Asp Glu Leu Thr Pro Ala

340 345

<210> 95

<211> 357

<212> PRT

<213> 发光类脐菇

<400> 95

Met Ile Ala Lys Asn Ser Glu Ile Asp Arg Phe Tyr Ile Pro Asp Thr

1 5 10 15

Leu Ala Asn Trp Pro Trp Pro Arg His Leu Asn Pro Ala Tyr Pro Glu

20 25 30

Ala Lys Lys Ala Ser Ala Ala Trp Leu Arg Ser Phe Asn Ala Phe Asn

35 40 45

Glu Arg Ser Gln Lys Ala Phe Asp Leu Cys Asp Phe Asn Leu Leu Ala

50 55 60

Ser Leu Ala Phe Pro Leu Ala Asp Leu Tyr Cys Leu Arg Ser Gly Cys

65 70 75 80

Asp Leu Met Asn Cys Phe Phe Ile Phe Asp Glu Tyr Ser Asp Val Ala

85 90 95

Asp Pro Gln Thr Val Arg Gln Gln Ala Asp Ile Ile Met Asp Ala Ile

100 105 110

Arg Asn Pro His Val Pro Arg Pro Arg Gly Glu Phe Ile Gly Gly Glu

115 120 125

Ala His Arg Gln Phe Trp Glu Arg Ala Met Gln Gly Ala Thr Pro Thr

130 135 140

Ala Gln Arg Arg Phe Ile Asp Thr Tyr Gln Gln Tyr Thr Asp Ala Val

145 150 155 160

Val Gln Gln Ala Thr Asp Arg Ala Asp Asn His Ile Arg Asp Val Glu

165 170 175

Gly Tyr Phe Thr Val Arg Arg Asp Thr Ile Gly Ala Lys Pro Ser Phe

180 185 190

Thr Leu Leu Glu Phe Thr Met Asp Ile Pro Asp Glu Val Met Gly His

195 200 205

Pro Val Ile Lys Asp Leu Ser Leu Trp Cys Ile Asp Met Leu Ile Ile

210 215 220

Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ala His Gly Asp Asp

225 230 235 240

Leu His Asn Leu Val Thr Ile Val Met Asn Gln Tyr Asn Leu Asp Leu

245 250 255

Pro Gly Ala Met Glu Trp Ile Gly Lys Phe His Asp Asp Ile Ala Asp

260 265 270

Lys Phe Leu Asp Thr Phe Ala Lys Leu Pro Ser Trp Gly Pro Glu Ile

275 280 285

Asp Pro Gln Ile Arg Arg Tyr Val Asp Gly Leu Gly Asn Trp Val Arg

290 295 300

Gly Asn Asp Ser Trp Ser Phe Glu Ser Trp Arg Tyr Phe Arg Gly Lys

305 310 315 320

Gly Pro Glu Ile Glu Lys Thr Arg Trp Val Asp Leu Met Pro Thr Glu

325 330 335

Glu Ala Thr Ile Thr Pro Lys Tyr Glu Ser Asp Ser Asn Ala Ala Gln

340 345 350

Pro Ala Gln Ser Thr

355

<210> 96

<211> 351

<212> PRT

<213> 毛韧革菌

<400> 96

Met Thr Val Val Asp Ser Pro Gln Arg Phe Tyr Ile Pro Asn Cys Leu

1 5 10 15

Glu Tyr Trp Pro Trp Pro Arg His Ile Asn Pro His Tyr Gln Glu Val

20 25 30

Lys Lys Ala Ser Ala Ala Trp Ala Glu Ser Phe Gly Ala Phe Asn Pro

35 40 45

Lys Ala Gln His Ala Tyr Asn Ala Cys Asp Phe Asn Leu Leu Ala Ser

50 55 60

Leu Ala Tyr Pro Leu Glu Ser Glu Glu Arg Leu Arg Thr Gly Cys Asp

65 70 75 80

Leu Met Asn Met Phe Phe Val Phe Asp Glu Tyr Ser Asp Val Ser Ser

85 90 95

Pro Lys Asp Val Ile Gln Gln Ala Ala Ile Ile Met Asp Ala Leu Arg

100 105 110

Asn Pro Tyr Ala Pro Arg Pro Asp Asp Glu Trp Val Gly Gly Glu Val

115 120 125

Thr Arg Gln Phe Trp Lys Arg Ala Ile Lys Thr Ala Thr Ala Gly Ala

130 135 140

Gln Arg Arg Phe Ile Asp Ala Phe Glu Ser Tyr Thr Gln Ser Val Val

145 150 155 160

Gln Gln Ala Lys Asp Arg His His Gly Phe Ile Arg Asp Val Asp Ser

165 170 175

Tyr Leu Glu Met Arg Arg Glu Thr Ile Gly Ala Lys Pro Ser Phe Val

180 185 190

Val Leu Gln Met Asp Met Thr Leu Pro Asp Glu Val Leu Ala His Pro

195 200 205

Val Ile Gln Gln Leu Ser Ala Leu Ser Thr Asp Met Ile Cys Leu Gly

210 215 220

Asn Asp Ile Cys Ser Tyr Asn Val Glu Gln Ala Arg Gly Asp Asp Leu

225 230 235 240

His Asn Ile Ile Thr Ile Ala Met Asn Gln Phe Asp Ile Asp Ile Ala

245 250 255

Gly Ala Met Asp Trp Val Val Lys Tyr His Ala Lys Leu Glu Arg Lys

260 265 270

Phe Leu Tyr Leu Tyr Asn Asn Gly Leu Pro Ser Trp Gly Lys Glu Leu

275 280 285

Asp Pro Gln Val Glu Arg Tyr Val Cys Gly Leu Gly Asn Trp Val Arg

290 295 300

Ala Ser Asp Gln Trp Gly Phe Glu Ser Glu Arg Tyr Phe Gly Lys Lys

305 310 315 320

Gly Lys Glu Ile Phe Lys Arg Arg Trp Val Asn Leu Met Gln Pro Glu

325 330 335

Arg Ala Gln Asp Ile Gly Pro Thr Leu Val Asp Gly Thr Arg Leu

340 345 350

<210> 97

<211> 345

<212> PRT

<213> 发光类脐菇

<400> 97

Met Pro Glu Thr Phe Tyr Leu Pro Asp Cys Leu Ala Asn Trp Lys Trp

1 5 10 15

Lys Arg Ala Leu Asn Pro Asn Tyr Pro Glu Val Lys Ala Ala Ser Ser

20 25 30

Glu Trp Leu Arg Ser Phe Lys Ala Phe Pro Pro Lys Ala Gln Glu Ala

35 40 45

Tyr Asp Arg Cys Asp Phe Asn Leu Leu Ala Ser Leu Ala Tyr Pro Leu

50 55 60

Ala Asp Lys Asp Gly Leu Arg Thr Gly Cys Asp Leu Met Asn Met Phe

65 70 75 80

Phe Val Phe Asp Glu Tyr Ser Asp Val Ala His Glu Ser Glu Val Gln

85 90 95

Val Gln Ala Asp Ile Ile Met Asp Ala Leu Arg Asn Pro His Lys Pro

100 105 110

Arg Pro Val Gly Glu Trp Val Gly Gly Glu Val Thr Arg Gln Phe Trp

115 120 125

Glu Leu Ala Ile Lys Thr Ala Ser Pro Gln Ser Gln Lys Arg Phe Ile

130 135 140

Glu Thr Phe Asp Thr Tyr Thr Lys Ser Val Val Gln Gln Ala Ala Asp

145 150 155 160

Arg Thr Gln His Tyr Val Arg Thr Val Asp Glu Tyr Leu Glu Val Arg

165 170 175

Arg Asp Thr Ile Gly Ala Lys Pro Ser Phe Ala Ile Leu Glu Leu Thr

180 185 190

Met Asp Ile Pro Asp Glu Val Ile His His Pro Thr Ile Glu Arg Leu

195 200 205

Ala Ile Leu Ala Ile Asp Met Ile Leu Leu Gly Asn Asp Thr Ala Ser

210 215 220

Tyr Asn Tyr Glu Gln Ala Arg Gly Asp Asp Asn His Asn Met Val Thr

225 230 235 240

Ile Val Met His Gln Tyr Lys Thr Asp Ile Gln Gly Ala Leu Ser Trp

245 250 255

Ile Glu Lys Tyr His Lys Glu Leu Glu Glu Glu Phe Met Gln Leu Tyr

260 265 270

Asn Ser Leu Pro Lys Trp Gly Gly Gln Ile Asp Val Asp Ile Ala Arg

275 280 285

Tyr Val Asp Gly Leu Gly Asn Trp Val Arg Ala Ser Asp Gln Trp Gly

290 295 300

Phe Glu Ser Glu Arg Tyr Phe Gly Thr Lys Ala Pro Glu Ile Gln Lys

305 310 315 320

Thr Arg Trp Val Thr Leu Met Pro Lys Lys Arg Ala Glu Gly Val Gly

325 330 335

Pro Glu Ile Val Asp Ile Ser Glu Leu

340 345

<210> 98

<211> 346

<212> PRT

<213> 高卢蜜环菌

<400> 98

Met Ser Gln Arg Ile Phe Leu Pro Asp Thr Leu Ala Asn Trp Gln Trp

1 5 10 15

Pro Arg His Leu Trp Pro His Tyr Ala Glu Val Lys Lys Ala Ser Ala

20 25 30

Ala Trp Ala Lys Ser Phe Arg Ala Phe Gln Thr Lys Ala Gln Glu Ala

35 40 45

Phe Asp Arg Cys Asp Phe Asn Leu Leu Ala Ser Phe Ala Tyr Pro Leu

50 55 60

Ala Asp Glu Ala Arg Leu Arg Ser Gly Cys Asp Leu Met Asn Leu Phe

65 70 75 80

Phe Val Ile Asp Glu Tyr Ser Asp Val Ser Thr Glu Glu Glu Val Arg

85 90 95

Ala Gln Lys Asp Ile Val Met Asp Ala Ile Arg Asn Thr Glu Lys Pro

100 105 110

Arg Pro Ala Gly Glu Trp Ile Gly Gly Glu Val Ser Arg Gln Phe Trp

115 120 125

Asp Leu Ala Lys Lys Thr Ala Ser Thr Gln Ala Gln Lys Arg Phe Ile

130 135 140

Asp Thr Phe Asp Glu Tyr Leu Glu Ser Val Val Gln Gln Ala Ala Asp

145 150 155 160

Arg Asn Asn Ser His Val Arg Gly Ile Glu Ser Tyr Leu Glu Val Arg

165 170 175

Arg Asn Thr Ile Gly Ala Lys Pro Ser Phe Ala Leu Leu Glu Phe Asp

180 185 190

Met Gln Leu Pro Asp Glu Ser His Gln Ser Ser Gly Tyr Gln Arg Asn

195 200 205

Leu Arg Lys Ser Cys Ile Asp Met Leu Cys Leu Gly Asn Asp Val Val

210 215 220

Ser Tyr Asn Leu Glu Gln Ala Arg Asp Asp Asp Gly His Asn Ile Val

225 230 235 240

Thr Ile Ala Met Asn Glu Leu Arg Thr Asp Val Ala Gly Ala Met Ile

245 250 255

Trp Val Asp Glu Tyr His Lys Gln Leu Glu Ser Arg Phe Met Glu Asn

260 265 270

Phe Lys Lys Val Pro Arg Trp Gly Gly Pro Ile Asp Leu Gln Val Ala

275 280 285

Arg Tyr Cys Asp Gly Leu Gly Asn Trp Val Arg Ala Asn Asp Gln Trp

290 295 300

Ser Phe Glu Ser Glu Arg Tyr Phe Gly Lys Lys Gly Pro Glu Ile Ile

305 310 315 320

Gln Arg Arg Trp Ile Thr Leu Met Pro Lys Met Val Ser Glu Glu Leu

325 330 335

Gly Pro Gln Ile Val Asp Gly Phe His Leu

340 345

<210> 99

<211> 369

<212> PRT

<213> 毛韧革菌

<400> 99

Met Ala Val Ala Thr Ser Val Ala Thr Pro Val Pro Thr Pro Ala Tyr

1 5 10 15

Ser Ala Gly Arg Ala Pro Ala Lys Glu Lys Lys Ile Tyr Leu Pro Asp

20 25 30

Thr Leu Ala Glu Trp Pro Trp Pro Arg Ala Ile Asn Pro His Tyr Ala

35 40 45

Glu Ala Lys Glu Glu Ser Gln Ala Trp Ala Ala Ser Phe Asn Ala Phe

50 55 60

Ser Pro Lys Ala Gln His Ala Phe Asn Arg Cys Asp Phe Asn Leu Leu

65 70 75 80

Ala Ser Leu Ala Tyr Pro Leu Ala Thr Lys His Gly Cys Arg Ser Gly

85 90 95

Cys Asp Leu Met Asn Leu Phe Phe Val Ile Asp Glu Tyr Ser Asp Ile

100 105 110

Ala Pro Val Glu Glu Val Arg Gln Gln Lys Asp Ile Val Met Asp Ala

115 120 125

Leu Arg Asn Pro His Lys Pro Arg Pro Glu Gly Glu Trp Val Gly Gly

130 135 140

Glu Val Ala Arg Gln Phe Trp Ala Leu Thr Ile Thr Asn Ala Ser Ala

145 150 155 160

Gln Ser Gln Lys His Phe Ile Glu Thr Phe Asp Glu Tyr Leu Asp Ser

165 170 175

Val Val Gln Gln Ala Glu Asp Arg Ser Glu Ser Arg Ile Arg Asp Ile

180 185 190

Gln Ser Tyr Ile Asp Val Arg Arg Asn Thr Ile Gly Ala Lys Pro Ser

195 200 205

Phe Ala Leu Leu Glu Leu Asp Met Asp Leu Pro Asp Glu Val Leu Ala

210 215 220

His Pro Thr Ile Gln Ser Leu Ser Leu Ala Thr Ile Asp Met Leu Cys

225 230 235 240

Leu Gly Asn Asp Ile Val Ser Tyr Asn Leu Glu Gln Ala Arg Gly Asp

245 250 255

Ala Ser His Asn Ile Ile Thr Ile Val Met Asn Glu Leu Asn Leu Asp

260 265 270

Val Asn Gly Ala Met Arg Trp Val Gly Asp Phe His Lys Gln Leu Glu

275 280 285

Lys Gln Phe Phe Glu Ala Phe Asn Asn Leu Pro Lys Trp Gly Asn Ala

290 295 300

Glu Leu Asp Ala Gln Ile Ala Val Tyr Cys Asp Gly Leu Gly Asn Trp

305 310 315 320

Val Arg Ala Asn Asp Gln Trp Ser Phe Glu Ser Glu Arg Tyr Phe Gly

325 330 335

Ala Arg Gly Leu Glu Ile Met Glu Thr Lys Thr Leu Ala Met Met Pro

340 345 350

Ile Gln Arg Thr Glu Ala Leu Gly Pro Gln Leu Val Asp Asp Ser Ile

355 360 365

Leu

<210> 100

<211> 350

<212> PRT

<213> 毛韧革菌

<400> 100

Met Val Arg Ser Pro Val Ser Asp Lys Phe Cys Ile Pro Asp Thr Leu

1 5 10 15

Ala Ser Trp Pro Tyr Pro Arg Ile Leu Asn Pro His Tyr Ala Glu Glu

20 25 30

Lys Ala Ala Ser Ala Ala Trp Thr Lys Gly Phe Gly Ala Phe Gly Pro

35 40 45

Lys Ala Gln Asp Ala Phe Asp Arg Cys Asp Phe Asn Leu Leu Ala Cys

50 55 60

Leu Ala Tyr Pro Ile Ala Thr Pro Glu Arg Cys Arg Ser Gly Cys Asp

65 70 75 80

Leu Met Asn Leu Phe Phe Val Ile Asp Glu His Ser Asp Thr His Gly

85 90 95

Glu Glu Thr Val Arg Lys Met Lys Asp Val Val Met Asp Ala Ile Arg

100 105 110

Asn Pro His Lys Pro Arg Pro Asn Asp Glu Trp Ile Gly Gly Glu Ile

115 120 125

Ala Arg Gln Phe Trp Glu Arg Ala Met Cys Tyr Ala Ser Glu Ile Ser

130 135 140

Gln Arg Arg Phe Ile Asp Thr Phe Asp Glu Tyr Leu Glu Ser Val Val

145 150 155 160

Asp Gln Ala Ala Asp Arg Asp Ser Ala Arg Ile Arg Asp Ile Glu Ser

165 170 175

Tyr Ile Asn Ile Arg Arg Asn Thr Ile Gly Ala Lys Pro Ser Phe Val

180 185 190

Ile Met Glu Gln Gly Met Asp Ile Pro Asp Asn Val Phe Glu Asn Glu

195 200 205

Val Phe Gln Arg Leu Arg Met Ala Thr Ile Asp Met Leu Cys Leu Gly

210 215 220

Asn Asp Ile Val Ser Tyr Asn Ile Glu Gln Ala Arg Gly Asp Asp Ser

225 230 235 240

His Asn Ile Val Arg Ile Val Met Asn Glu Leu Asp Thr Asp Val Pro

245 250 255

Arg Ala Met Asp Trp Val Ala Gln Arg His Thr Gln Leu Glu Arg Glu

260 265 270

Phe Phe Thr Ala Leu Ser Glu Leu Pro Thr Trp Gly Glu Pro Ile Asp

275 280 285

Gly Trp Val Lys Glu Tyr Val Tyr Gly Leu Gly Asn Trp Val Arg Ala

290 295 300

Asn Asp Gln Trp Ser Phe Glu Ser Gln Arg Tyr Phe Gly Thr Lys Gly

305 310 315 320

Met Glu Ile Met Lys Ser Arg Trp Leu Ser Val Leu Pro Lys Val Arg

325 330 335

Pro Ala Glu Val Gly Pro Gln Leu Val Asp Gln Ser Leu Leu

340 345 350

<210> 101

<211> 330

<212> PRT

<213> 灰盖鬼伞

<400> 101

Met Val Gly Ser Tyr Thr Gly Lys Val Ile His Val Pro Ala Leu Leu

1 5 10 15

Glu Ser Trp Pro Trp Pro Ala Ala Ile Asn Pro Leu Tyr Glu Gln Val

20 25 30

Gln Glu Glu Ser Thr Ser Trp Phe Arg Lys Phe Asp Leu Tyr Arg Asp

35 40 45

Arg Lys Lys Gln Ala Ile His Asp His Leu Asp Thr Ala Lys Phe Gly

50 55 60

Ala Ser Val Cys Pro Lys Ala Asp Tyr Ala Leu Leu Arg Leu Ala Thr

65 70 75 80

Asp Tyr Leu His Leu Gly Phe Trp Ile Asp Tyr Phe Phe Asp Thr Ser

85 90 95

Pro Ser Asp Val Ile Arg Gln Leu Thr Glu Ser Ile Ala His Leu Leu

100 105 110

Glu Ser Gly Asp Pro Arg Leu Asp Ser Ser Ser Pro Gln Ser His Ile

115 120 125

Ala Cys Met Glu Ile Leu Arg Asp Phe Arg Lys Arg Ile Glu Thr Phe

130 135 140

Asn Pro Ser Gln Glu Asp Leu Arg Arg Phe Val Lys Glu Tyr Arg Gly

145 150 155 160

Phe Leu Glu Ala Glu Leu Thr Gln Ala Ile Asp His Glu Asn Lys Val

165 170 175

Ile Arg Asp Ile Glu Ser Tyr Leu Ser Ile Arg Arg Ser Thr Ile Ala

180 185 190

Ile Arg Pro Gly Ile Ala Leu Leu Gly Leu Ala Leu Gly Ile Pro Gln

195 200 205

Glu Ile Leu Asp Asp Pro Tyr Thr Asp Thr Leu Thr Asn Ala Cys Leu

210 215 220

Asp Met Val Ile Ile Gln Asn Asp Ala Tyr Ser Trp Asn Val Glu Gln

225 230 235 240

Val Arg Lys Ala Asp Gly His Asn Ile Ile Thr Val Leu Met Lys Gln

245 250 255

Arg Asp Ile Asp Val Gln Glu Ala Tyr Glu His Ala Ala Gln Leu His

260 265 270

Arg Glu Thr Gln Glu His Phe Leu Glu Leu His Ala Lys Arg Pro Asp

275 280 285

Trp Gly Asn Glu Gly Ser Ile Gln Ala Phe Phe Asp Gly Leu Gly Glu

290 295 300

Phe Val Arg Gly Val Asp Glu Trp Ser Ser Met Cys Leu Gly Glu His

305 310 315 320

Ala Leu Ser Val Gly Ala Gly Phe Leu Lys

325 330

<210> 102

<211> 372

<212> PRT

<213> 毛韧革菌

<400> 102

Met Glu Ser Val Arg Glu His Ile Pro Arg Leu Gln His Phe Leu Gly

1 5 10 15

Glu Ile Gly Tyr Arg His Thr Thr Pro Pro Ala Pro Thr Leu Asp Phe

20 25 30

Leu His Ala His His His Trp Ile His His Val Leu Gly Pro Met Thr

35 40 45

Ser Trp Thr Val Ala Lys Leu Asn Ala Leu Glu Asp Ser Ser Ser Thr

50 55 60

Ile Phe Glu Arg Ala Tyr Pro Leu Ser Asp Ala Glu Met Lys Phe Val

65 70 75 80

Leu Ala Lys Leu Thr Ala Ile Ala Ile Phe Leu Asp Asp Ser Leu Glu

85 90 95

Asp Glu Glu Thr Tyr Asp Asp Ile Gly Asn Phe Ala His Arg Val Tyr

100 105 110

Leu Gly Glu Ala Gln Pro Thr Gly Val Leu Thr Leu Tyr His Gln Gly

115 120 125

Ile Gln Glu Leu Ser Lys Met His Glu Gly Asp Ala Val Phe Arg Gly

130 135 140

Leu Ala Val Ala Pro Trp Ile Thr Phe Ile Asp Ala Cys Met Leu Glu

145 150 155 160

Lys Arg Leu Leu Thr Phe Asp Ser Lys Leu Arg Val Ser Pro Arg Asp

165 170 175

Leu Gly Tyr Gln Arg Leu Arg Asn Ser Thr Asp Phe Thr Ser Leu Arg

180 185 190

Ala Pro Lys Ala Thr Pro Ser Glu Val Glu Val Ser Phe Pro Ile Phe

195 200 205

Leu Arg His Lys Ser Gly Ile Gly Glu Ala Tyr Ala Ala Ala Ile Phe

210 215 220

Lys Ser Ser Arg Tyr Gln Glu Leu Pro Leu Ser Arg Phe Val Lys Ser

225 230 235 240

Met Pro Asp Met Ile Tyr Tyr Ile Glu Leu Val Asn Asp Leu Met Ser

245 250 255

Phe Tyr Lys Glu Gln Leu Ala Gly Glu Thr Ala Asn Leu Ile His Leu

260 265 270

Gln His Gln Ser Trp Lys Gly Gly Gln Gly Thr Gly Pro Tyr Gly Ser

275 280 285

Trp Thr Leu Leu Asp Thr Phe Ser Arg Leu Cys Asp Glu Thr Arg Asp

290 295 300

Ala Ala Phe Arg Val Asp Glu Leu Leu Arg Leu Asp Glu Cys Glu Lys

305 310 315 320

Ile Ala Asn Gly Glu Leu Arg Gly Glu Glu Val Gly Leu Ser Pro Met

325 330 335

Asp Val Thr Met Ala Ala Gln Trp Arg Glu Phe Arg Asp Gly Tyr Val

340 345 350

Ser Trp His Leu Glu Cys Gln Arg Tyr Lys Leu Asp Phe Ile Lys Leu

355 360 365

Ser Thr Phe Glu

370

<210> 103

<211> 325

<212> PRT

<213> 灰盖鬼伞

<400> 103

Met Pro Ala Ala Leu Pro Tyr Asn Val Ser Arg Asp Asn Lys Trp Asp

1 5 10 15

Ile Lys Lys Ile Ile Gln Asp Phe Phe Lys Arg Cys Asp Val Pro Tyr

20 25 30

Gln Val Ile Pro Tyr Asp Thr Glu Leu Trp Asn Ala Cys Leu Lys Arg

35 40 45

Ala Lys Glu Lys Gly Tyr Pro Val Glu Pro Asp Ser Pro Met Ser Leu

50 55 60

Tyr Arg Ser Phe Lys Val Gly Val Val Ile Thr Arg Thr Ser Tyr Gly

65 70 75 80

His Ile Gln Asp Tyr Glu Ile Leu Ile Trp Val Ala Thr Phe Thr Ala

85 90 95

Phe Val Thr Tyr Ala Asp Asp Ala Phe Gln Glu Asp Ile Gln His Leu

100 105 110

His Ser Phe Ala Arg Thr Phe Leu Gln Asn Glu Lys His Glu His Pro

115 120 125

Val Leu Glu Ala Phe Ala Gln Phe Leu Arg Glu Ser Ser Ile Arg Phe

130 135 140

Ser His Phe Val Ala Asn Thr Val Val Ser Ser Ala Leu Arg Phe Met

145 150 155 160

Met Ser Ile Ala Leu Glu Phe Glu Gly Gln Asn Val Ser Val Ser Thr

165 170 175

Glu Ala Arg Glu Tyr Pro Gly Tyr Ile Arg Ile Leu Ser Gly Leu Ser

180 185 190

Asp Ile Tyr Ala Leu Phe Ala Phe Pro Met Asp Leu Pro Arg Ser Thr

195 200 205

Tyr Ile Gln Ala Phe Pro Glu Gln Ile Asp Tyr Ile Asn Gly Thr Asn

210 215 220

Asp Leu Leu Ser Phe Tyr Lys Glu Glu Leu Asp Cys Glu Thr Val Asn

225 230 235 240

Phe Ile Ser Ala Ala Ala Thr Ser Gln Gln Val Ser Lys Leu Glu Val

245 250 255

Leu Arg Asn Ala Ala Glu Lys Ala Ala Tyr Ser Tyr Asp Val Val Val

260 265 270

Asn Val Leu Lys Pro Tyr Pro Glu Ala Leu Ala Ala Trp Lys Ser Phe

275 280 285

Ala Arg Gly Phe Cys Tyr Phe His Thr Ser Ser Pro Arg Tyr Arg Leu

290 295 300

Gly Glu Met Phe His Asp Phe Glu His Asp Leu Val Cys Lys Cys Ala

305 310 315 320

Ser Cys Thr Glu Ile

325

<210> 104

<211> 328

<212> PRT

<213> 发光类脐菇

<400> 104

Met Thr Leu Pro Thr Glu Gln Val Glu Leu Ser Val Cys Pro Val Glu

1 5 10 15

Ser Ser Thr His Thr Thr Arg Asp Ile Met Arg Asn Phe Leu Ser Gln

20 25 30

Cys Gln Ile Pro Leu Gln Arg Gly Val Pro Leu Asp Pro Thr Phe His

35 40 45

Gln Glu Cys Ala Asn Val Leu Ile Glu Asp Tyr Leu Lys Pro Ser Ala

50 55 60

Ala Val Thr Leu Glu Asn Leu Pro Ser Leu Met Ser Ser Phe Asn Pro

65 70 75 80

Phe Leu Thr Leu Gly Val Arg Met Ala Ser Thr Gly Tyr Ala His Leu

85 90 95

Thr His Thr Pro Thr Arg Val Tyr Val Ala Leu Phe Thr Ala Leu Leu

100 105 110

Val Cys Leu Asp Asp Ile Phe Pro Glu Asn Val Glu Leu Met Cys Gly

115 120 125

Phe Asn Glu Arg Phe Ile Lys Asn Glu Thr Gln Gly Glu Pro Ile Leu

130 135 140

Asp Ala Val Ala Gly Leu Leu Arg Ser Thr Ser Lys Tyr Phe Ser Met

145 150 155 160

Leu Ser Ser Asn Leu Ile Val Thr Ser Ala Leu Asn Tyr Val Thr Ser

165 170 175

Leu Ser Leu Asp Gln Gly Leu His Ser Ile Lys Leu Ala Glu His Ser

180 185 190

Arg Asn Phe Ala Arg Leu Cys Arg Asn Met Ser Gly Ile Pro Glu Ala

195 200 205

Phe Ala Ala Phe Val Phe Pro Pro Glu Val Pro Phe Thr Ala Tyr Ile

210 215 220

Gln Cys Phe Pro Asp Leu Tyr Thr Tyr Ala Asn Tyr Val Asn Asp Val

225 230 235 240

Leu Ser Phe Tyr Lys Glu Asp Ile Ala Gly Glu Thr Glu Asn Leu Val

245 250 255

Ser Ile Leu Ala Gln Thr Gln Pro Asn Ser Ser Arg Tyr Gln Val Leu

260 265 270

Gln Gln Leu Ala Asp Glu Ala Ala Ala Ala Asn Ala Asn Ile Arg Asp

275 280 285

Ile Leu Ser Asp Gln Lys Ser Ile Leu Asp Ala Tyr Asp Ala Phe Arg

290 295 300

Val Gly Phe Val Gln Phe His Ile Asp Ser Pro Arg Tyr Arg Leu Ala

305 310 315 320

Glu Leu Phe Pro Cys Ile Asp Gly

325

<210> 105

<211> 350

<212> PRT

<213> 发光类脐菇

<400> 105

Met Ser Gln Ile Leu His Leu Leu Trp Ser Lys Phe Ser Thr Ser Leu

1 5 10 15

Pro Ser Thr Val Thr Ile Gly Ser Asp Pro Gln Thr Leu Gln Leu Val

20 25 30

His Ser Pro Ala Pro Asn Val Asn Ala Asn Ala Leu Glu Ile Tyr Lys

35 40 45

Ile Val Asp Asn Phe Leu Ser Arg Cys Gly Ile Arg Leu Glu Ser Thr

50 55 60

Pro Leu Asp Val Glu Phe Tyr Asn Glu Cys Lys Lys Thr Leu Leu Ser

65 70 75 80

His Tyr Ile Gly Ile His Asp Ser Asp Lys Val Ser Glu Ser Trp Phe

85 90 95

Lys Arg Tyr Leu Ser Val Gly Val Ile Ile Thr Thr Asn Ala Tyr Gly

100 105 110

His Leu Asp Asn Lys Leu Thr Lys Ile Tyr Ile Ala Leu Tyr Thr Ala

115 120 125

Leu Ala Thr Cys Phe Asp Asp Val Phe Glu Lys Asn Val Asp His Met

130 135 140

Ser Gly Phe Asn Glu Arg Phe Met Lys Ala Leu Pro Gln Gly Asp Val

145 150 155 160

Phe Leu Asp Ala Phe Ala Lys Val Leu Leu Asp Ala Pro Lys Tyr Phe

165 170 175

Gly Arg Leu Ala Ser Asn Ile Ile Val Thr Ser Thr Leu Asp Phe Ile

180 185 190

Thr Ser Met Ser Val Asp Val Leu Thr Lys Gly Met Lys Phe Asn Gln

195 200 205

Asn Leu His Lys Phe Ala Met Ala Cys Arg Asn Met Ser Gly Ile Ala

210 215 220

Tyr Thr Tyr Ala Pro Phe Ile Phe Pro Lys Glu Val Pro Phe Ala Ile

225 230 235 240

Tyr Ala Gln Cys Leu Pro Asp Met Arg Ile Tyr Ile Asn His Val Asn

245 250 255

Asp Val Leu Ser Phe Tyr Lys Glu Asp Lys Ala Gly Glu Thr Glu Asn

260 265 270

Leu Ala Ser Ile Leu Gly Gln Val His Pro Ser Met Thr Lys Tyr Gln

275 280 285

Ile Val Gln Gly Leu Ala Asp Asp Ala Ala Glu Ala Asp Leu Arg Val

290 295 300

Arg Thr Val Leu Ser Gln Tyr Gln Pro Ala Leu Asp Ala Tyr Asn Cys

305 310 315 320

Phe Arg Gln Gly Tyr Val Ser Phe His Ala Ser Ser Gly Arg Tyr Arg

325 330 335

Leu Asp Glu Leu Phe Ser Phe Val Glu Pro Glu Pro Ile Val

340 345 350

<210> 106

<211> 347

<212> PRT

<213> 发光类脐菇

<400> 106

Met Ser Gln Ile Leu His Leu Leu Trp Ser Lys Phe Ser Thr Ser Leu

1 5 10 15

Pro Ser Thr Val Thr Ile Gly Ser Asp Pro Gln Thr Leu Gln Leu Val

20 25 30

His Ser Pro Ala Pro Asn Val Asn Ala Asn Ala Leu Glu Ile Arg Arg

35 40 45

Ile Val Asn Asn Phe Leu Thr Arg Cys Ser Ile Arg Leu Glu Gly Thr

50 55 60

Pro Leu Asp Val Asp Phe Tyr Asn Glu Cys Lys Lys Thr Leu Leu Ser

65 70 75 80

His Tyr Ile Gly Ile His Asp Ser Asp Lys Val Ser Glu Ser Trp Phe

85 90 95

Arg Arg Tyr Leu Ser Val Gly Val Ile Ile Thr Thr Asn Ala Tyr Gly

100 105 110

His Ile Asp Asp Lys Pro Thr Arg Val Tyr Ile Cys Leu Tyr Thr Ala

115 120 125

Leu Leu Thr Ser Phe Asp Asp Val Phe Glu Ala Asn Val Glu Gln Phe

130 135 140

Gly Gly Phe Asn Glu Arg Phe Met Lys Gly Glu Pro Gln Glu Asp Leu

145 150 155 160

Phe Leu Asp Ala Leu Ala Arg Ile Leu Leu Asp Ala Pro Arg Tyr Tyr

165 170 175

Gly Arg Leu Ala Thr Asn Ile Ile Val Thr Ala Thr Leu Asp Phe Phe

180 185 190

Thr Gly Leu Phe Leu Glu Leu Gln Ala Arg Asp Met Thr Phe Asn Glu

195 200 205

Asp Leu His Asn Phe Ala Val Phe Cys Arg Asn Leu Thr Gly Ile Ala

210 215 220

His Ala Tyr Ala Val Phe Met Phe Pro Arg Asp Val Pro Phe Thr Val

225 230 235 240

Tyr Val Arg Ser Leu Pro Glu Leu Lys Thr Asp Ile Asn Tyr Val Asn

245 250 255

Asp Ile Met Ser Phe Tyr Lys Glu Asp Arg Ala Glu Glu Thr Asp Asn

260 265 270

Leu Ala Ser Ile Leu Arg Gln Val His Pro Ser Met Thr Lys His Gln

275 280 285

Val Leu Gln Lys Ile Val Asp Asp Ala Val Glu Ala Asp Val Arg Ala

290 295 300

Arg Lys Ile Leu Ala Asp Tyr Gln Pro Ala Leu Asp Ala Tyr Glu His

305 310 315 320

Phe Arg Lys Gly Tyr Ala Met Phe His Val Ser Ser Gly Arg Tyr Arg

325 330 335

Leu Asp Glu Leu Phe Ser Tyr Ile Arg Phe Glu

340 345

<210> 107

<211> 17

<212> PRT

<213> 香灰菌

<400> 107

Met Ala Lys Ala Asn Leu Leu Ile Phe Met His Asp Asp Val Cys Glu

1 5 10 15

Tyr

<210> 108

<211> 21

<212> PRT

<213> 香灰菌

<400> 108

Phe Ser Leu Thr Asn Asp Leu Tyr Ser Phe Asn Lys Glu Val Val Ala

1 5 10 15

Glu Gln Glu Thr Gly

20

<210> 109

<211> 16

<212> PRT

<213> 香灰菌

<400> 109

Val Trp Asp Asp Glu Val Asp Ala Asn Asp Thr Asp Val Ser Asn Asp

1 5 10 15

<210> 110

<211> 21

<212> PRT

<213> 香灰菌

<400> 110

Cys Phe Ile Leu Asn Asp Val Tyr Ser Val Gln Lys Glu Ile Ala Gln

1 5 10 15

Asp Ser Leu Leu Asn

20

<210> 111

<211> 17

<212> PRT

<213> 香灰菌

<400> 111

Ala Trp Asp Asp Glu Thr Asp Ser Pro Glu Phe Ser Ala Val Ile Asn

1 5 10 15

Asp

<210> 112

<211> 21

<212> PRT

<213> 香灰菌

<400> 112

Ile His Thr Thr Asn Asp Ile Leu Ser Val Lys Lys Glu Val Ala Gln

1 5 10 15

Ser Gln Val Asp Ser

20

<210> 113

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 113

His Leu Asp Asn Ile Ser Asp Gly Met Met

1 5 10

<210> 114

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 114

Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Asn Val Glu Gln Ser Arg

1 5 10 15

Gly Asp Thr His Asn

20

<210> 115

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 115

His Leu Asp Asn Ile Ser Asp Gly Met Met

1 5 10

<210> 116

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 116

Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Asn Val Glu Gln Ser Arg

1 5 10 15

Gly Asp Thr His Asn

20

<210> 117

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 117

His Leu Asp Asn Ile Ser Asp Gly Met Met

1 5 10

<210> 118

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 118

Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Asn Val Glu Gln Ser Lys

1 5 10 15

Gly Asp Thr His Asn

20

<210> 119

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 119

His Leu Asp Asn Leu Ser Asp Glu Met Asp

1 5 10

<210> 120

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 120

Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Ser Val Glu Gln Ser Lys

1 5 10 15

Gly His Thr His Asn

20

<210> 121

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 121

His Ile Asp Asn Leu Ser Asp Asp Met Asp

1 5 10

<210> 122

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 122

Val Thr Trp Ser Asn Asp Ile Phe Ser Phe Asn Val Glu Gln Ser Lys

1 5 10 15

Gly Asp Thr His Asn

20

<210> 123

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 123

Tyr Leu Asp Asn Leu Thr Asp Asp Met Asp

1 5 10

<210> 124

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 124

Val Ser Phe Ala Asn Asp Ile Tyr Ser Phe Asn Ile Glu Gln Ser Lys

1 5 10 15

Gly Asp Thr His Asn

20

<210> 125

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 125

His Leu Asp Asn Leu Ser Asp Asp Met Asp

1 5 10

<210> 126

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 126

Val Thr Trp Ser Asn Asp Ile Phe Ser Tyr Asn Val Glu Gln Ala Lys

1 5 10 15

Gly Asp Thr His Asn

20

<210> 127

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 127

Asn Ala Asp Asp Trp Leu Asp Asp Phe Asp

1 5 10

<210> 128

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 128

Ile Ala Trp Ser Asn Asp Ile Phe Ser Phe Asn Arg Glu Gln Ser Arg

1 5 10 15

His Asp Ser Phe Asn

20

<210> 129

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 129

Phe Phe Asp Glu Val Thr Asp Thr Glu Thr

1 5 10

<210> 130

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 130

Ile Phe Leu Ala Asn Asp Leu Tyr Ser Tyr Asn Met Glu Gln Ala Lys

1 5 10 15

Gly His Asn Gly Ala

20

<210> 131

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 131

Leu Tyr Asp Glu Tyr Thr Asp Thr Gly Ser

1 5 10

<210> 132

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 132

Val Phe Trp Ala Asn Asp Leu Val Ser Tyr Asn Met Glu Gln Ser Lys

1 5 10 15

Gly His Ser Gly Ala

20

<210> 133

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 133

Val Val Asp Glu Val Ser Asp Glu Gln Ser

1 5 10

<210> 134

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 134

Val Cys Trp Ala Asn Asp Val Tyr Ser Tyr Asp Met Glu Gln Ser Lys

1 5 10 15

Gly Leu Ser Asn Asn

20

<210> 135

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 135

Val Val Asp Glu Ile Ser Asp Asp Gln Asn

1 5 10

<210> 136

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 136

Val Cys Trp Ala Asn Asp Val Tyr Ser Tyr Asp Met Glu Gln Ala Lys

1 5 10 15

Gly His Thr Gly Asn

20

<210> 137

<211> 10

<212> PRT

<213> 毛韧革菌

<400> 137

Val Ile Asp Glu Ile Ser Asp Asp Gln Ser

1 5 10

<210> 138

<211> 21

<212> PRT

<213> 毛韧革菌

<400> 138

Val Cys Trp Ala Asn Asp Val Tyr Ser Tyr Asn Val Glu Gln Ala Lys

1 5 10 15

Gly His Ser Gly Asn

20

<210> 139

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 139

Val Leu Asp Glu Val Ser Asp Glu Gln Ser

1 5 10

<210> 140

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 140

Val Cys Trp Ala Asn Asp Val Tyr Ser Tyr Asn Lys Glu Gln Ala Gln

1 5 10 15

Gly His Gly Gly Asn

20

<210> 141

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 141

Val Leu Asp Glu Leu Ser Asp Asp Gln Asn

1 5 10

<210> 142

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 142

Val Cys Trp Ser Asn Asp Val Tyr Ser Tyr Asn Val Glu Gln Ala Lys

1 5 10 15

Gly His Gly Gly Ser

20

<210> 143

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 143

Val Phe Asp Glu Val Ser Asp Asp Gln Asn

1 5 10

<210> 144

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 144

Val Cys Trp Ser Asn Asp Val Tyr Ser Tyr Asn Val Glu Gln Ala Lys

1 5 10 15

Gly His Arg Gly Ser

20

<210> 145

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 145

Val Tyr Asp Glu Tyr Thr Asp Val Ser Asp

1 5 10

<210> 146

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 146

Ile Ile Leu Val Asn Asp Met His Ser Tyr Val Arg Glu Leu Ser Cys

1 5 10 15

Gly His Glu Asn His

20

<210> 147

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 147

Val Tyr Asp Glu Tyr Thr Asp Val Val Ser

1 5 10

<210> 148

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 148

Thr Arg Val Ser Ser Gly Gln Ala Asn His

1 5 10

<210> 149

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 149

Val Phe Asp Glu Tyr Thr Asp Ile Ala Asp

1 5 10

<210> 150

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 150

Ile Ala Ile Gly Asn Asp Ile Asp Ser Tyr Ala Met Glu Lys Ala Arg

1 5 10 15

Gly Leu Glu Leu His

20

<210> 151

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 151

Val Phe Asp Glu Cys Ala Asp Ile Ala Asp

1 5 10

<210> 152

<211> 12

<212> PRT

<213> 柱状田头菇

<400> 152

Asp Ile Asn Ser Tyr Pro Met Lys Val Arg Gly Leu

1 5 10

<210> 153

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 153

Val Phe Asp Glu Trp Ser Asp Val Ser Asp

1 5 10

<210> 154

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 154

Leu Cys Ile Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ser Arg

1 5 10 15

Gly Asp Asp Gly His

20

<210> 155

<211> 10

<212> PRT

<213> 柱状田头菇

<400> 155

Val Val Asp Glu His Thr Asp Ala Met Asp

1 5 10

<210> 156

<211> 21

<212> PRT

<213> 柱状田头菇

<400> 156

Leu Ile Leu Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ser Arg

1 5 10 15

Gly Asp Asp Gly His

20

<210> 157

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 157

Ile Phe Asp Glu Tyr Ser Asp Val Ala Asp

1 5 10

<210> 158

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 158

Leu Ile Ile Gly Asn Asp Leu Cys Ser Tyr Asn Val Glu Gln Ala His

1 5 10 15

Gly Asp Asp Leu His

20

<210> 159

<211> 10

<212> PRT

<213> 毛韧革菌

<400> 159

Val Phe Asp Glu Tyr Ser Asp Val Ser Ser

1 5 10

<210> 160

<211> 21

<212> PRT

<213> 毛韧革菌

<400> 160

Ile Cys Leu Gly Asn Asp Ile Cys Ser Tyr Asn Val Glu Gln Ala Arg

1 5 10 15

Gly Asp Asp Leu His

20

<210> 161

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 161

Val Phe Asp Glu Tyr Ser Asp Val Ala His

1 5 10

<210> 162

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 162

Ile Leu Leu Gly Asn Asp Thr Ala Ser Tyr Asn Tyr Glu Gln Ala Arg

1 5 10 15

Gly Asp Asp Asn His

20

<210> 163

<211> 10

<212> PRT

<213> 高卢蜜环菌

<400> 163

Val Ile Asp Glu Tyr Ser Asp Val Ser Thr

1 5 10

<210> 164

<211> 21

<212> PRT

<213> 高卢蜜环菌

<400> 164

Leu Cys Leu Gly Asn Asp Val Val Ser Tyr Asn Leu Glu Gln Ala Arg

1 5 10 15

Asp Asp Asp Gly His

20

<210> 165

<211> 10

<212> PRT

<213> 毛韧革菌

<400> 165

Val Ile Asp Glu Tyr Ser Asp Ile Ala Pro

1 5 10

<210> 166

<211> 21

<212> PRT

<213> 毛韧革菌

<400> 166

Leu Cys Leu Gly Asn Asp Ile Val Ser Tyr Asn Leu Glu Gln Ala Arg

1 5 10 15

Gly Asp Ala Ser His

20

<210> 167

<211> 10

<212> PRT

<213> 毛韧革菌

<400> 167

Val Ile Asp Glu His Ser Asp Thr His Gly

1 5 10

<210> 168

<211> 21

<212> PRT

<213> 毛韧革菌

<400> 168

Leu Cys Leu Gly Asn Asp Ile Val Ser Tyr Asn Ile Glu Gln Ala Arg

1 5 10 15

Gly Asp Asp Ser His

20

<210> 169

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 169

Trp Ile Asp Tyr Phe Phe Asp Thr Ser Pro

1 5 10

<210> 170

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 170

Val Ile Ile Gln Asn Asp Ala Tyr Ser Trp Asn Val Glu Gln Val Arg

1 5 10 15

Lys Ala Asp Gly His

20

<210> 171

<211> 10

<212> PRT

<213> 毛韧革菌

<400> 171

Leu Asp Asp Ser Leu Glu Asp Glu Glu Thr

1 5 10

<210> 172

<211> 21

<212> PRT

<213> 毛韧革菌

<400> 172

Ile Glu Leu Val Asn Asp Leu Met Ser Phe Tyr Lys Glu Gln Leu Ala

1 5 10 15

Gly Glu Thr Ala Asn

20

<210> 173

<211> 10

<212> PRT

<213> 灰盖鬼伞

<400> 173

Ala Phe Val Thr Tyr Ala Asp Asp Ala Phe

1 5 10

<210> 174

<211> 21

<212> PRT

<213> 灰盖鬼伞

<400> 174

Ile Asn Gly Thr Asn Asp Leu Leu Ser Phe Tyr Lys Glu Glu Leu Asp

1 5 10 15

Cys Glu Thr Val Asn

20

<210> 175

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 175

Ala Leu Leu Val Cys Leu Asp Asp Ile Phe

1 5 10

<210> 176

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 176

Ala Asn Tyr Val Asn Asp Val Leu Ser Phe Tyr Lys Glu Asp Ile Ala

1 5 10 15

Gly Glu Thr Glu Asn

20

<210> 177

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 177

Ala Leu Ala Thr Cys Phe Asp Asp Val Phe

1 5 10

<210> 178

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 178

Ile Asn His Val Asn Asp Val Leu Ser Phe Tyr Lys Glu Asp Lys Ala

1 5 10 15

Gly Glu Thr Glu Asn

20

<210> 179

<211> 10

<212> PRT

<213> 发光类脐菇

<400> 179

Ala Leu Leu Thr Ser Phe Asp Asp Val Phe

1 5 10

<210> 180

<211> 21

<212> PRT

<213> 发光类脐菇

<400> 180

Ile Asn Tyr Val Asn Asp Ile Met Ser Phe Tyr Lys Glu Asp Arg Ala

1 5 10 15

Glu Glu Thr Asp Asn

20