一种融合蛋白及其应用
文献发布时间：2023-06-19 11:26:00
技术领域
本发明涉及生物医药领域，具体而言，涉及一种融合蛋白及其应用。
背景技术
所有病毒都有核酸(包括DNA和RNA)，并且都以核酸作为寄生存活的根本。目前应对病毒传染病(例如新冠病毒、HIV等等)主要寄希望于疫苗。疫苗开发难度大，时间长，效果也因人而异，在特殊情况下，疫苗可能不起应有的作用甚至起反作用。故有必要开发一种以直接杀灭病毒为主的组合型预防和治疗病毒疾病的方法。
有鉴于此，特提出本发明。
发明内容
这种方法的关键特点是通过蛋白融合(核酸酶-病毒识别蛋白融合体)或者载体结合(核酸酶-病毒识别载体)的方式，将一般性降解病毒核酸的工具酶与病毒的特异性受体以及抗体有机结合在一起，直接针对性地杀灭特定病毒。这种方法以核酸酶降解病毒核酸为主攻武器，以病毒的特异性受体以及抗体为主要开关，配合以病毒特异性融膜分子、病毒特异性融膜载体(包括脂质体、纳米颗粒、红细胞，功能性活细胞等)作为病毒攻击通道。
本方法使用的核酸酶包括所有能够降解病毒核酸的工具酶，例如各种RNase，DNase，ribozyme，siRNA，CRISPR介导的DNA酶和RNA酶等等。
本方法使用的病毒的特异性受体以及抗体包括所有能够与特定病毒组成成分特异性能够结合的一般分子，其中特别包括病毒的受体以及所有能够与特定病毒组成成分特异性结合的抗体，例如新冠病毒的受体ACE2以及各种抗新冠病毒S蛋白，E蛋白，M蛋白，N蛋白抗体等等。
本方法使用的蛋白融合(核酸酶-病毒识别蛋白融合体)方式包括将核酸酶通过共价键融合连接到各种特定病毒识别蛋白体的N端或者C端。例如特定病毒受体的N端或者C端，以及将核酸酶通过共价键融合连接到特定病毒抗体轻链的N端或者C端，或者连接到特定病毒抗体重链的N端或者C端。
本方法使用的病毒特异性融膜载体包括脂质体、纳米颗粒、红细胞，各种功能性活细胞等等。
本方法使用的载体结合(核酸酶-病毒识别载体)方式包括将针对特定病毒核酸的核酸酶放入病毒特异性融膜载体内部，将特定病毒识别蛋白体(例如病毒受体和抗体等等)放到病毒特异性融膜载体表面。
在使用各种功能性活细胞作为核酸酶-病毒识别载体的方式中，一般采用基因表达载体的方式将针对特定病毒中特定核酸序列的特异性核酸酶(例如ribozyme，siRNA,CRISPR介导的DNA酶和RNA酶等等)放入病毒特异性融膜载体内部，将特定病毒识别蛋白体(例如病毒受体和抗体等等)放到病毒特异性融膜载体表面。
本发明采用了如下技术方案：
本发明提供一种预防和治疗病毒性传染病的融合蛋白，其特征在于，包括：核酸酶、病毒抗体或病毒受体。
进一步，本发明的预防和治疗病毒性传染病的融合蛋白，其特征在于，还包括：病毒特异性融膜载体。
本发明还提供一种预防和治疗新冠状病毒传染病的融合蛋白，其特征在于，包括：核酸酶、新冠病毒抗体或病毒受体。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于，包括：新冠病毒受体。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于：所述新冠病毒受体为ACE2及其改造形式，新冠病毒抗体为CR3022及其改造形式。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于，还包括：核糖核酸酶。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于：所述核糖核酸酶为RNase7、RNase2、RNaseK、RNaseT2、XRN2、XRN1或新冠状病毒RNA序列特异性的RNaseP。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于，还包括：co-receptors(受体)：为CD147、DC-SIGN(CD209)、L-SIGN(CD209L)、CLEC4G、ANPEP、ENPEP或DPP4。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，其特征在于，融合蛋白的序列如本文二十三个实施例(1-23)所示。
进一步，本发明的预防和治疗新冠状病毒传染病的融合蛋白，融合蛋白为新冠病毒受体-新冠病毒抗体-核糖核酸酶形式。
本发明还提供一种识别新冠病毒的RNA酶-红细胞载体，首先通过基因表达载体将新冠病毒的受体ACE2m(H374D,H378D)和CD147以及抗新冠病毒的抗体(IgM完整形式)表达在人脐带血中的CD34阳性细胞hematopoietic stem cells(HSCs)表面，然后诱导生成成熟的无核红细胞，然后采用短暂低渗透压的方法将RNase7，RNase2，XRN2，XRN1放到红细胞内部。
本发明还提供一种识别新冠病毒的RNA酶-功能性活细胞载体，首先通过基因表达载体将新冠病毒的受体ACE2m(H374D,H378D)，CD147，抗新冠病毒的抗体，以及专门识别并且特异性切断新冠病毒RNA保守序列的RNase P在人脐带血或者其他有核细胞中表达。
本发明的预防和治疗病毒性传染病的多功能融合蛋白和多功能载体，开发速度比疫苗更快而且更容易，安全性和有效性把握性更大。可以推广应用到各种病毒传染病的预防和治疗中。
具体实施方式
下面以新冠病毒为主进行具体说明，当然，相似的蛋白结构和开发方式开发所得到的成果可以应用到其它各种细胞特异性病毒传染病。
实施例一
本实施例的融合蛋白ACE2-huFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染，融合蛋白序列如下：
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
本实施例及以下一些实施例中，每个融合蛋白分子的第一行是用于细胞分泌的信号肽，最终的纯化产品不包括信号肽。如果用昆虫细胞表达可以换成gp64或者gp67信号肽,如果用细菌表达需要将信号肽替换成起始密码。融合蛋白的各部分以GSGG或者GGSGG的连接序列进行连接。
实施例二
本实施例的融合蛋白ACE2-huFc(G1+M)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染，融合蛋白序列如下：
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例三
本实施例的融合蛋白ACE2m(H374D,H378D)-huFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：(ACE2序列中374位的H，修改为D，378位的H，也修改为D):
MSSSSWLLLSLVAVTAAQ(信号肽)
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHdEMGdIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例四
本实施例的融合蛋白ACE2m(H374D,H378D)-huFc(G1+M)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：(ACE2序列中374位的H，修改为D，378位的H，也修改为D):
MSSSSWLLLSLVAVTAAQ(信号肽)
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHdEMGdIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例五
本实施例的融合蛋白ACE2-huFc(G1)-RNase2，用于鼻腔和眼睛点滴而预防新冠病毒传染，融合蛋白序列如下：
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
SLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTF
LLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDN
RDQRRDPPQYPVVPVHLDRII
实施例六
本实施例的融合蛋白ACE2-huFc(G1+M)-RNase2，用于鼻腔和眼睛点滴而预防新冠病毒传染，融合蛋白序列如下：
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
GGSGG
SLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTF
LLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDN
RDQRRDPPQYPVVPVHLDRII
实施例七
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+Anti-SARS-CoV2-hK-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
Anti-SARS-CoV2-hK-RNase7:
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例八
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+M+Anti-SARS-CoV2-hK-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1+M:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
Anti-SARS-CoV2-hK-RNase7:
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例九
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+Anti-SARS-CoV2-hK-RNaseK，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
Anti-SARS-CoV2-hK-RNaseK:
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
GGSGG
MASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYNLYEQVSYNCF
IAAGLYLLLGGFSFCQVRLNKRKEYMVR
实施例十
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+M+Anti-SARS-CoV2-hK-RNaseK，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1+M:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
Anti-SARS-CoV2-hK-RNaseK:
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
GGSGG
MASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYNLYEQVSYNCF
IAAGLYLLLGGFSFCQVRLNKRKEYMVR
实施例十一
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+Anti-SARS-CoV2-hK-ribonucleaseT2，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
Anti-SARS-CoV2-hK-ribonuclease T2:
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
GGSGG
MRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINY
YQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGA
AESRGLRVCEDGPVFYPPPKKTKH
实施例十二
本实施例的融合蛋白Anti-SARS-CoV2-hIgG1+RNase2-Anti-SARS-CoV2-hK，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
Anti-SARS-CoV2-hIgG1:
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTI
SADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
ASTKGPSVFPLAPSSK
STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS
SLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF
SCSVMHEALHNHYTQKSLSLSPGK
RNase2-Anti-SARS-CoV2-hK:
SLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTF
LLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDN
RDQRRDPPQYPVVPVHLDRII
GGSGG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSG
SGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSST
LTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
实施例十三
本实施例的融合蛋白ACE2p1-CR3022vLvH-hFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRIL
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例十四
本实施例的融合蛋白ACE2 p1m1-CR3022vLvH-hFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDLFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRIL
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例十五
本实施例的融合蛋白ACE2p1-CR3022vHvL-CD147-hFc(G1)-RNase2，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRIL
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGSGGGGSGGGSGGGSGGSG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
SLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTF
LLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDN
RDQRRDPPQYPVVPVHLDRII
实施例十六
本实施例的融合蛋白ACE2 p1m2-CR3022vHvL-CD147-hFc(G1)-RNase2，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNFEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRIL
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGSGGGGSGGGSGGGSGGSG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
SLHVKPPQFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTF
LLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDN
RDQRRDPPQYPVVPVHLDRII
实施例十七
本实施例的融合蛋白ACE2 p2m1-CR3022vLvH-hFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDLFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例十八
本实施例的融合蛋白ACE2p2-CR3022vLvH-hFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例十九
本实施例的融合蛋白ACE2 p2 m-CR3022vLvH-CD147-hFc(G1)-RNase7用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDLFNFEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例二十
本实施例的融合蛋白ACE2p2-CR3022vLvH-CD147-hFc(G1)-RNase7，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGSGGGGSGGGSGGGSGGSG
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
实施例二十一
本实施例的融合蛋白ACE2 p2m2-CR3022vHvL-CD147-hFc(G1)-RNaseT2，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNFEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGSGGGGSGGGSGGGSGGSG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
MRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINY
YQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGA
AESRGLRVCEDGPVFYPPPKKTKH
实施例二十二
本实施例的融合蛋白ACE2p2-CR3022vHvL-CD147-hFc(G1)-RNaseT2，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGSGGGGSGGGSGGGSGGSG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGG
MRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINY
YQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGA
AESRGLRVCEDGPVFYPPPKKTKH
实施例二十三
本实施例的融合蛋白ACE2p2m-CR3022vHvL-CD147-hFc(G1)-RNaseK，用于鼻腔和眼睛点滴而预防新冠病毒传染或者静脉注射治疗，融合蛋白序列如下：
MGS
STIEEQAKTFLDLFNFEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTG
GGGGSGGGGSGGGGS
QMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GGSGGGGSGGGSGGGSGGSG
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKR
GGGSGGGSGGGSGGG
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
GGGSGGGSGGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
GGSGGG
MASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYNLYEQVSYNCF
IAAGLYLLLGGFSFCQVRLNKRKEYMVR
实施例二十四
识别新冠病毒的RNA酶-红细胞载体
在GMP条件下，首先通过基因表达载体将新冠病毒的受体ACE2m(H374D,H378D)和CD147以及抗新冠病毒的抗体(IgM完整形式)表达在人脐带血中的CD34阳性细胞hematopoietic stem cells(HSCs)表面，然后诱导生成成熟的无核红细胞。然后采用短暂低渗透压的方法将RNase7，RNase2，XRN2，XRN1放到红细胞内部。组装好的细胞经过鉴定和政府批准后静脉注射用于治疗新冠病毒传染病人。
>NP_001304889.1 5'-3'exoribonuclease 2isoform 1[Homo sapiens]XRN2
MGSAACPRGALPELAPCCQPREQSQPHTRWDAGCGIQHPGGEEFRTLGGARAYRVPNSQEGRSSPTRFFP
APEGPAHCFVSSPDRAFWVSEEVQRLLLSNACQPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIHPC
THPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEAAVEK
QRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKNLTVILSDASAPG
EGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHE
VKDCEGLPREKKGKHDELADSLPCAEGEFIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFV
GNDFLPHLPSLEIRENAIDRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDED
SFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPSISPNTSFTS
DGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQSYVEGLCWVLRY
YYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMGVFPAASGNFLPPSWRKLMSDPD
SSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPL
HDFILELYQTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAE
DYIFKAVMLPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYS
NAAPPPVTYQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNA
AFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN
>NP_001269786.1 5'-3'exoribonuclease 1 isoform c[Homo sapiens]XRN1
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEV
LFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFM
ARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLI
MLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIID
DWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREH
FSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET
EDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLS
DIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEW
EAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYK
IISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDE
LTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNW
AKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFD
SRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIK
YNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYS
SAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQ
ILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPV
GLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVK
PQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVL
PQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKE
NEVQSSHHGEPPSEEHLSPQSFAMKGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQ
PKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTM
TVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPT
ANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKE
AQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHHKSTPISSSRRKS
RKLAVNFGVSKPSE
>BAC76828.1 CD147[Homo sapiens]
MAAALFVLLGFALLGTHGASG
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQ
KTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWY
KITDSE
DKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALW
PLLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS
ACE2m(H374D,H378D)
MSSSSWLLLSLVAVTAAQ
STIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWS
AFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE
CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVN
GVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYS
LTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHdEMGdIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
IGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEP
VPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL
GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD
QSIKVRISLKSALGD
RAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEV
EKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
IWLIVFGVVMGVIVVGIVILIFTGIRDRKK
KNKARSGENPYASIDISKGENNPGFQNTDDVQTSF
SMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNAT
FSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITC
LVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALP
NRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY
KPKGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNT
FLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCRYKEKRQNKSYVVACKPPQKK
DSQQFHLVPVHLDRVL
Anti-SARS-CoV2-scFv-huFc(G-M)
MPLLLLLPLLWAGALA
DIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKGGSGGGGSGGGSGGGGSGGGSGMQMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVSS
GSGG
DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI
EKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDG
SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
PDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSIL
TVSEEEWNTGETYTCVVAHEALPNRVTERTVD
KSTGKPTLYNVSLVMSDTAGTCY
实施例二十五
识别新冠病毒的RNA酶-功能性活细胞载体
在GMP条件下，首先通过基因表达载体将新冠病毒的受体ACE2m(H374D,H378D)，CD147，抗新冠病毒的抗体，以及专门识别并且特异性切断新冠病毒RNA保守序列的RNase P在人脐带血或者其他合适的有核细胞中表达。组装好的细胞经过鉴定和政府批准后静脉注射用于治疗新冠病毒传染病人。
ACE2m(H374D,H378D)，CD147，抗新冠病毒的抗体序列与上面相同，专门识别并且特异性切断新冠病毒RNA保守序列的RNase P序列如下：
(用U6或者H1 promoter表达)
M1(G33C G205C C326T)RNaseP RdRp1
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
CAAAGAAGAAGTG CCA
M1(G33C G205C C326T)RNaseP RdRp2
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
CTGATATCACACAT CCA
M1(G33C G205C C326T)RNaseP RdRp3
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
TCTGATCCCAATAT CCA
M1(G33C G205C C326T)RNaseP RdRp4
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
GTCTCTTAACTACA CCA
M1(G33C G205C C326T)RNaseP RdRp5
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
GCGAGCTCTATTCT CCA
M1(G33C G205C C326T)RNaseP RdRp6
GAAGCTGACCAGACAGTCGCCGCTTCGTCGTCcTCCTCTTCGGGGGAGACGGGCGGAGGGGAGGAAAGTCCGGGCTCCATAGGGCAGGGTGCCAGGTAACGCCTGGGGGGGAAACCCACGACCAGTGCAACAGAGAGCAAACCGCCGATGGCCCGCGCAAGCGGGATCAGGTAAGGGTGAAAGGGTGCGGTAagAGCGCACCGCcCGGCTGGTAACAGTCCGTGGCACGGTAAACTCCACCCGGAGCAAGGCCAAATAGGGGTTCATAAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCtTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCT
CACACTCATTAGCT CCA
尽管已用具体实施例来说明和描述了本发明，然而应意识到，在不背离本发明的精神和范围的情况下可以作出许多其它的更改和修改。因此，这意味着在所附权利要求中包括属于本发明范围内的所有这些变化和修改。
SEQUENCE LISTING
<110> 维亚生物科技（上海）有限公司
<120> 一种融合蛋白及其应用
<130> JSP12001319
<160> 39
<170> PatentIn version 3.3
<210> 1
<211> 1086
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 1
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys Val
625 630 635 640
Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro Pro
705 710 715 720
Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro
725 730 735
Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
740 745 750
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
755 760 765
Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr
770 775 780
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
785 790 795 800
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
805 810 815
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
820 825 830
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
835 840 845
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
850 855 860
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
865 870 875 880
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
885 890 895
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
900 905 910
Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
915 920 925
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
930 935 940
Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly Lys Pro
945 950 955 960
Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His Met Gln Pro
965 970 975
Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn Lys His Thr
980 985 990
Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro Phe Ser Ser
995 1000 1005
Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys Asn Gly
1010 1015 1020
Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr Met
1025 1030 1035
Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu
1040 1045 1050
Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln
1055 1060 1065
Lys Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp
1070 1075 1080
Arg Val Leu
1085
<210> 2
<211> 1211
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 2
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys Val
625 630 635 640
Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro Pro
705 710 715 720
Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro
725 730 735
Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
740 745 750
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
755 760 765
Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr
770 775 780
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
785 790 795 800
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
805 810 815
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
820 825 830
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
835 840 845
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
850 855 860
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
865 870 875 880
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
885 890 895
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
900 905 910
Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
915 920 925
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
930 935 940
Lys Ser Leu Ser Leu Ser Pro Gly Lys Pro Asp Val Tyr Leu Leu Pro
945 950 955 960
Pro Ala Arg Glu Gln Leu Asn Leu Arg Glu Ser Ala Thr Ile Thr Cys
965 970 975
Leu Val Thr Gly Phe Ser Pro Ala Asp Val Phe Val Gln Trp Met Gln
980 985 990
Arg Gly Gln Pro Leu Ser Pro Glu Lys Tyr Val Thr Ser Ala Pro Met
995 1000 1005
Pro Glu Pro Gln Ala Pro Gly Arg Tyr Phe Ala His Ser Ile Leu
1010 1015 1020
Thr Val Ser Glu Glu Glu Trp Asn Thr Gly Glu Thr Tyr Thr Cys
1025 1030 1035
Val Val Ala His Glu Ala Leu Pro Asn Arg Val Thr Glu Arg Thr
1040 1045 1050
Val Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr Asn Val Ser Leu
1055 1060 1065
Val Met Ser Asp Thr Ala Gly Thr Cys Tyr Gly Gly Ser Gly Gly
1070 1075 1080
Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His
1085 1090 1095
Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile
1100 1105 1110
Asn Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His
1115 1120 1125
Glu Pro Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile
1130 1135 1140
Ala Cys Lys Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala
1145 1150 1155
Val Ser Leu Thr Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn
1160 1165 1170
Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser Tyr Val Val Ala
1175 1180 1185
Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe His Leu Val
1190 1195 1200
Pro Val His Leu Asp Arg Val Leu
1205 1210
<210> 3
<211> 1104
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 3
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala
1 5 10 15
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe
20 25 30
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp
35 40 45
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn
50 55 60
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala
65 70 75 80
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln
85 90 95
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys
100 105 110
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser
115 120 125
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu
130 135 140
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu
145 150 155 160
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu
165 170 175
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg
180 185 190
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu
195 200 205
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu
210 215 220
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu
225 230 235 240
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile
245 250 255
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly
260 265 270
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys
275 280 285
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala
290 295 300
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu
305 310 315 320
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro
325 330 335
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly
340 345 350
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp
355 360 365
Phe Leu Thr Ala His Asp Glu Met Gly Asp Ile Gln Tyr Asp Met Ala
370 375 380
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe
385 390 395 400
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
405 410 415
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn
420 425 430
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly
435 440 445
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe
450 455 460
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met
465 470 475 480
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr
485 490 495
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe
500 505 510
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala
515 520 525
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile
530 535 540
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu
545 550 555 560
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala
565 570 575
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe
580 585 590
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr
595 600 605
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu
610 615 620
Lys Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met
625 630 635 640
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu
645 650 655
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val
660 665 670
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro
675 680 685
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile
690 695 700
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn
705 710 715 720
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln
725 730 735
Pro Pro Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro
740 745 750
Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
755 760 765
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
770 775 780
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
785 790 795 800
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
805 810 815
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
820 825 830
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
835 840 845
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
850 855 860
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
865 870 875 880
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
885 890 895
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
900 905 910
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
915 920 925
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
930 935 940
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
945 950 955 960
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly
965 970 975
Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His Met
980 985 990
Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn Lys
995 1000 1005
His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro
1010 1015 1020
Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys
1025 1030 1035
Lys Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser
1040 1045 1050
Leu Thr Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg
1055 1060 1065
Tyr Lys Glu Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys
1070 1075 1080
Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe His Leu Val Pro Val
1085 1090 1095
His Leu Asp Arg Val Leu
1100
<210> 4
<211> 1229
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 4
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala
1 5 10 15
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe
20 25 30
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp
35 40 45
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn
50 55 60
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala
65 70 75 80
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln
85 90 95
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys
100 105 110
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser
115 120 125
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu
130 135 140
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu
145 150 155 160
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu
165 170 175
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg
180 185 190
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu
195 200 205
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu
210 215 220
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu
225 230 235 240
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile
245 250 255
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly
260 265 270
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys
275 280 285
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala
290 295 300
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu
305 310 315 320
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro
325 330 335
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly
340 345 350
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp
355 360 365
Phe Leu Thr Ala His Asp Glu Met Gly Asp Ile Gln Tyr Asp Met Ala
370 375 380
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe
385 390 395 400
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
405 410 415
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn
420 425 430
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly
435 440 445
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe
450 455 460
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met
465 470 475 480
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr
485 490 495
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe
500 505 510
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala
515 520 525
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile
530 535 540
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu
545 550 555 560
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala
565 570 575
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe
580 585 590
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr
595 600 605
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu
610 615 620
Lys Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met
625 630 635 640
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu
645 650 655
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val
660 665 670
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro
675 680 685
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile
690 695 700
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn
705 710 715 720
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln
725 730 735
Pro Pro Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro
740 745 750
Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
755 760 765
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
770 775 780
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
785 790 795 800
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
805 810 815
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
820 825 830
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
835 840 845
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
850 855 860
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
865 870 875 880
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
885 890 895
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
900 905 910
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
915 920 925
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
930 935 940
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
945 950 955 960
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Pro Asp Val Tyr Leu
965 970 975
Leu Pro Pro Ala Arg Glu Gln Leu Asn Leu Arg Glu Ser Ala Thr Ile
980 985 990
Thr Cys Leu Val Thr Gly Phe Ser Pro Ala Asp Val Phe Val Gln Trp
995 1000 1005
Met Gln Arg Gly Gln Pro Leu Ser Pro Glu Lys Tyr Val Thr Ser
1010 1015 1020
Ala Pro Met Pro Glu Pro Gln Ala Pro Gly Arg Tyr Phe Ala His
1025 1030 1035
Ser Ile Leu Thr Val Ser Glu Glu Glu Trp Asn Thr Gly Glu Thr
1040 1045 1050
Tyr Thr Cys Val Val Ala His Glu Ala Leu Pro Asn Arg Val Thr
1055 1060 1065
Glu Arg Thr Val Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr Asn
1070 1075 1080
Val Ser Leu Val Met Ser Asp Thr Ala Gly Thr Cys Tyr Gly Gly
1085 1090 1095
Ser Gly Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys
1100 1105 1110
Ile Gln His Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met
1115 1120 1125
Lys Asn Ile Asn Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr
1130 1135 1140
Phe Leu His Glu Pro Phe Ser Ser Val Ala Ala Thr Cys Gln Thr
1145 1150 1155
Pro Lys Ile Ala Cys Lys Asn Gly Asp Lys Asn Cys His Gln Ser
1160 1165 1170
His Gly Ala Val Ser Leu Thr Met Cys Lys Leu Thr Ser Gly Lys
1175 1180 1185
His Pro Asn Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser Tyr
1190 1195 1200
Val Val Ala Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe
1205 1210 1215
His Leu Val Pro Val His Leu Asp Arg Val Leu
1220 1225
<210> 5
<211> 1096
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 5
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys Val
625 630 635 640
Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro Pro
705 710 715 720
Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro
725 730 735
Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
740 745 750
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
755 760 765
Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr
770 775 780
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
785 790 795 800
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
805 810 815
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
820 825 830
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
835 840 845
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
850 855 860
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
865 870 875 880
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
885 890 895
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
900 905 910
Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
915 920 925
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
930 935 940
Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly Ser Leu
945 950 955 960
His Val Lys Pro Pro Gln Phe Thr Trp Ala Gln Trp Phe Glu Thr Gln
965 970 975
His Ile Asn Met Thr Ser Gln Gln Cys Thr Asn Ala Met Gln Val Ile
980 985 990
Asn Asn Tyr Gln Arg Arg Cys Lys Asn Gln Asn Thr Phe Leu Leu Thr
995 1000 1005
Thr Phe Ala Asn Val Val Asn Val Cys Gly Asn Pro Asn Met Thr
1010 1015 1020
Cys Pro Ser Asn Lys Thr Arg Lys Asn Cys His His Ser Gly Ser
1025 1030 1035
Gln Val Pro Leu Ile His Cys Asn Leu Thr Thr Pro Ser Pro Gln
1040 1045 1050
Asn Ile Ser Asn Cys Arg Tyr Ala Gln Thr Pro Ala Asn Met Phe
1055 1060 1065
Tyr Ile Val Ala Cys Asp Asn Arg Asp Gln Arg Arg Asp Pro Pro
1070 1075 1080
Gln Tyr Pro Val Val Pro Val His Leu Asp Arg Ile Ile
1085 1090 1095
<210> 6
<211> 1221
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 6
Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe Asn His
1 5 10 15
Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp Asn Tyr
20 25 30
Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn Ala Gly
35 40 45
Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala Gln Met
50 55 60
Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln Leu Gln
65 70 75 80
Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys Ser Lys
85 90 95
Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser Thr Gly
100 105 110
Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu Glu Pro
115 120 125
Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu Arg Leu
130 135 140
Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu Arg Pro
145 150 155 160
Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg Ala Asn
165 170 175
His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu Val Asn
180 185 190
Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu Asp Val
195 200 205
Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu His Ala
210 215 220
Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile Ser Pro
225 230 235 240
Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly Arg Phe
245 250 255
Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys Pro Asn
260 265 270
Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala Gln Arg
275 280 285
Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu Pro Asn
290 295 300
Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro Gly Asn
305 310 315 320
Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly Lys Gly
325 330 335
Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp Phe Leu
340 345 350
Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala Tyr Ala
355 360 365
Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe His Glu
370 375 380
Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys His Leu
385 390 395 400
Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn Glu Thr
405 410 415
Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly Thr Leu
420 425 430
Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe Lys Gly
435 440 445
Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met Lys Arg
450 455 460
Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr Tyr Cys
465 470 475 480
Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe Ile Arg
485 490 495
Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala Leu Cys
500 505 510
Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile Ser Asn
515 520 525
Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu Gly Lys
530 535 540
Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala Lys Asn
545 550 555 560
Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe Thr Trp
565 570 575
Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr Asp Trp
580 585 590
Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu Lys Ser
595 600 605
Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met Tyr Leu
610 615 620
Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu Lys Val
625 630 635 640
Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val Ala Asn
645 650 655
Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro Lys Asn
660 665 670
Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile Arg Met
675 680 685
Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn Ser Leu
690 695 700
Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln Pro Pro
705 710 715 720
Val Ser Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro
725 730 735
Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
740 745 750
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
755 760 765
Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr
770 775 780
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
785 790 795 800
Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
805 810 815
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
820 825 830
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
835 840 845
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu
850 855 860
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
865 870 875 880
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
885 890 895
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
900 905 910
Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
915 920 925
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
930 935 940
Lys Ser Leu Ser Leu Ser Pro Gly Lys Pro Asp Val Tyr Leu Leu Pro
945 950 955 960
Pro Ala Arg Glu Gln Leu Asn Leu Arg Glu Ser Ala Thr Ile Thr Cys
965 970 975
Leu Val Thr Gly Phe Ser Pro Ala Asp Val Phe Val Gln Trp Met Gln
980 985 990
Arg Gly Gln Pro Leu Ser Pro Glu Lys Tyr Val Thr Ser Ala Pro Met
995 1000 1005
Pro Glu Pro Gln Ala Pro Gly Arg Tyr Phe Ala His Ser Ile Leu
1010 1015 1020
Thr Val Ser Glu Glu Glu Trp Asn Thr Gly Glu Thr Tyr Thr Cys
1025 1030 1035
Val Val Ala His Glu Ala Leu Pro Asn Arg Val Thr Glu Arg Thr
1040 1045 1050
Val Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr Asn Val Ser Leu
1055 1060 1065
Val Met Ser Asp Thr Ala Gly Thr Cys Tyr Gly Gly Ser Gly Gly
1070 1075 1080
Ser Leu His Val Lys Pro Pro Gln Phe Thr Trp Ala Gln Trp Phe
1085 1090 1095
Glu Thr Gln His Ile Asn Met Thr Ser Gln Gln Cys Thr Asn Ala
1100 1105 1110
Met Gln Val Ile Asn Asn Tyr Gln Arg Arg Cys Lys Asn Gln Asn
1115 1120 1125
Thr Phe Leu Leu Thr Thr Phe Ala Asn Val Val Asn Val Cys Gly
1130 1135 1140
Asn Pro Asn Met Thr Cys Pro Ser Asn Lys Thr Arg Lys Asn Cys
1145 1150 1155
His His Ser Gly Ser Gln Val Pro Leu Ile His Cys Asn Leu Thr
1160 1165 1170
Thr Pro Ser Pro Gln Asn Ile Ser Asn Cys Arg Tyr Ala Gln Thr
1175 1180 1185
Pro Ala Asn Met Phe Tyr Ile Val Ala Cys Asp Asn Arg Asp Gln
1190 1195 1200
Arg Arg Asp Pro Pro Gln Tyr Pro Val Val Pro Val His Leu Asp
1205 1210 1215
Arg Ile Ile
1220
<210> 7
<211> 449
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 7
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys
<210> 8
<211> 353
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 8
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
1 5 10 15
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
20 25 30
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
85 90 95
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
100 105 110
Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp
115 120 125
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
130 135 140
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu
145 150 155 160
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp
165 170 175
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr
180 185 190
Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser
195 200 205
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser Gly
210 215 220
Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His
225 230 235 240
Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn
245 250 255
Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro
260 265 270
Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys
275 280 285
Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr
290 295 300
Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu
305 310 315 320
Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln Lys
325 330 335
Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp Arg Val
340 345 350
Leu
<210> 9
<211> 574
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 9
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys Pro Asp Val Tyr Leu Leu Pro Pro Ala Arg Glu Gln Leu Asn Leu
450 455 460
Arg Glu Ser Ala Thr Ile Thr Cys Leu Val Thr Gly Phe Ser Pro Ala
465 470 475 480
Asp Val Phe Val Gln Trp Met Gln Arg Gly Gln Pro Leu Ser Pro Glu
485 490 495
Lys Tyr Val Thr Ser Ala Pro Met Pro Glu Pro Gln Ala Pro Gly Arg
500 505 510
Tyr Phe Ala His Ser Ile Leu Thr Val Ser Glu Glu Glu Trp Asn Thr
515 520 525
Gly Glu Thr Tyr Thr Cys Val Val Ala His Glu Ala Leu Pro Asn Arg
530 535 540
Val Thr Glu Arg Thr Val Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr
545 550 555 560
Asn Val Ser Leu Val Met Ser Asp Thr Ala Gly Thr Cys Tyr
565 570
<210> 10
<211> 353
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 10
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
1 5 10 15
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
20 25 30
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
85 90 95
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
100 105 110
Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp
115 120 125
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
130 135 140
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu
145 150 155 160
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp
165 170 175
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr
180 185 190
Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser
195 200 205
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser Gly
210 215 220
Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His
225 230 235 240
Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn
245 250 255
Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro
260 265 270
Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys
275 280 285
Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr
290 295 300
Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu
305 310 315 320
Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln Lys
325 330 335
Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp Arg Val
340 345 350
Leu
<210> 11
<211> 449
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 11
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys
<210> 12
<211> 323
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 12
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
1 5 10 15
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
20 25 30
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
85 90 95
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
100 105 110
Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp
115 120 125
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
130 135 140
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu
145 150 155 160
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp
165 170 175
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr
180 185 190
Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser
195 200 205
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser Gly
210 215 220
Gly Met Ala Ser Leu Leu Cys Cys Gly Pro Lys Leu Ala Ala Cys Gly
225 230 235 240
Ile Val Leu Ser Ala Trp Gly Val Ile Met Leu Ile Met Leu Gly Ile
245 250 255
Phe Phe Asn Val His Ser Ala Val Leu Ile Glu Asp Val Pro Phe Thr
260 265 270
Glu Lys Asp Phe Glu Asn Gly Pro Gln Asn Ile Tyr Asn Leu Tyr Glu
275 280 285
Gln Val Ser Tyr Asn Cys Phe Ile Ala Ala Gly Leu Tyr Leu Leu Leu
290 295 300
Gly Gly Phe Ser Phe Cys Gln Val Arg Leu Asn Lys Arg Lys Glu Tyr
305 310 315 320
Met Val Arg
<210> 13
<211> 574
<212> PRT
<213> Artificial
<220>
<223> fuison protein
<400> 13
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys Pro Asp Val Tyr Leu Leu Pro Pro Ala Arg Glu Gln Leu Asn Leu
450 455 460
Arg Glu Ser Ala Thr Ile Thr Cys Leu Val Thr Gly Phe Ser Pro Ala
465 470 475 480
Asp Val Phe Val Gln Trp Met Gln Arg Gly Gln Pro Leu Ser Pro Glu
485 490 495
Lys Tyr Val Thr Ser Ala Pro Met Pro Glu Pro Gln Ala Pro Gly Arg
500 505 510
Tyr Phe Ala His Ser Ile Leu Thr Val Ser Glu Glu Glu Trp Asn Thr
515 520 525
Gly Glu Thr Tyr Thr Cys Val Val Ala His Glu Ala Leu Pro Asn Arg
530 535 540
Val Thr Glu Arg Thr Val Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr
545 550 555 560
Asn Val Ser Leu Val Met Ser Asp Thr Ala Gly Thr Cys Tyr
565 570
<210> 14
<211> 323
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 14
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
1 5 10 15
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
20 25 30
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
85 90 95
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
100 105 110
Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp
115 120 125
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
130 135 140
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu
145 150 155 160
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp
165 170 175
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr
180 185 190
Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser
195 200 205
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser Gly
210 215 220
Gly Met Ala Ser Leu Leu Cys Cys Gly Pro Lys Leu Ala Ala Cys Gly
225 230 235 240
Ile Val Leu Ser Ala Trp Gly Val Ile Met Leu Ile Met Leu Gly Ile
245 250 255
Phe Phe Asn Val His Ser Ala Val Leu Ile Glu Asp Val Pro Phe Thr
260 265 270
Glu Lys Asp Phe Glu Asn Gly Pro Gln Asn Ile Tyr Asn Leu Tyr Glu
275 280 285
Gln Val Ser Tyr Asn Cys Phe Ile Ala Ala Gly Leu Tyr Leu Leu Leu
290 295 300
Gly Gly Phe Ser Phe Cys Gln Val Arg Leu Asn Lys Arg Lys Glu Tyr
305 310 315 320
Met Val Arg
<210> 15
<211> 449
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 15
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys
<210> 16
<211> 389
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 16
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
1 5 10 15
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
20 25 30
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
35 40 45
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
50 55 60
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65 70 75 80
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
85 90 95
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
100 105 110
Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp
115 120 125
Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
130 135 140
Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu
145 150 155 160
Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp
165 170 175
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr
180 185 190
Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser
195 200 205
Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser Gly
210 215 220
Gly Met Arg Ala Tyr Trp Pro Asp Val Ile His Ser Phe Pro Asn Arg
225 230 235 240
Ser Arg Phe Trp Lys His Glu Trp Glu Lys His Gly Thr Cys Ala Ala
245 250 255
Gln Val Asp Ala Leu Asn Ser Gln Lys Lys Tyr Phe Gly Arg Ser Leu
260 265 270
Glu Leu Tyr Arg Glu Leu Asp Leu Asn Ser Val Leu Leu Lys Leu Gly
275 280 285
Ile Lys Pro Ser Ile Asn Tyr Tyr Gln Val Ala Asp Phe Lys Asp Ala
290 295 300
Leu Ala Arg Val Tyr Gly Val Ile Pro Lys Ile Gln Cys Leu Pro Pro
305 310 315 320
Ser Gln Asp Glu Glu Val Gln Thr Ile Gly Gln Ile Glu Leu Cys Leu
325 330 335
Thr Lys Gln Asp Gln Gln Leu Gln Asn Cys Thr Glu Pro Gly Glu Gln
340 345 350
Pro Ser Pro Lys Gln Glu Val Trp Leu Ala Asn Gly Ala Ala Glu Ser
355 360 365
Arg Gly Leu Arg Val Cys Glu Asp Gly Pro Val Phe Tyr Pro Pro Pro
370 375 380
Lys Lys Thr Lys His
385
<210> 17
<211> 449
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 17
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
1 5 10 15
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
20 25 30
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
35 40 45
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
50 55 60
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
65 70 75 80
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
85 90 95
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
100 105 110
Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe
115 120 125
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
130 135 140
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
145 150 155 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
165 170 175
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
180 185 190
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
195 200 205
Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys
210 215 220
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
225 230 235 240
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
245 250 255
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
260 265 270
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
275 280 285
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
290 295 300
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
305 310 315 320
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
325 330 335
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
340 345 350
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr
355 360 365
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
370 375 380
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
385 390 395 400
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
405 410 415
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
420 425 430
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
435 440 445
Lys
<210> 18
<211> 363
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 18
Ser Leu His Val Lys Pro Pro Gln Phe Thr Trp Ala Gln Trp Phe Glu
1 5 10 15
Thr Gln His Ile Asn Met Thr Ser Gln Gln Cys Thr Asn Ala Met Gln
20 25 30
Val Ile Asn Asn Tyr Gln Arg Arg Cys Lys Asn Gln Asn Thr Phe Leu
35 40 45
Leu Thr Thr Phe Ala Asn Val Val Asn Val Cys Gly Asn Pro Asn Met
50 55 60
Thr Cys Pro Ser Asn Lys Thr Arg Lys Asn Cys His His Ser Gly Ser
65 70 75 80
Gln Val Pro Leu Ile His Cys Asn Leu Thr Thr Pro Ser Pro Gln Asn
85 90 95
Ile Ser Asn Cys Arg Tyr Ala Gln Thr Pro Ala Asn Met Phe Tyr Ile
100 105 110
Val Ala Cys Asp Asn Arg Asp Gln Arg Arg Asp Pro Pro Gln Tyr Pro
115 120 125
Val Val Pro Val His Leu Asp Arg Ile Ile Gly Gly Ser Gly Gly Asp
130 135 140
Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu
145 150 155 160
Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser
165 170 175
Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro
180 185 190
Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro
195 200 205
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile
210 215 220
Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr
225 230 235 240
Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
245 250 255
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu
260 265 270
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe
275 280 285
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln
290 295 300
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser
305 310 315 320
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu
325 330 335
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser
340 345 350
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
355 360
<210> 19
<211> 1009
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 19
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu
115 120 125
Leu Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn
130 135 140
Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln
145 150 155 160
Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala
165 170 175
Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr
180 185 190
Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile
195 200 205
Glu Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His
210 215 220
Leu His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr
225 230 235 240
Ile Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp
245 250 255
Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln
260 265 270
Lys Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp
275 280 285
Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly
290 295 300
Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp
305 310 315 320
Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu
325 330 335
Gly Lys Gly Asp Phe Arg Ile Leu Gly Gly Gly Gly Ser Gly Gly Gly
340 345 350
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
355 360 365
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
370 375 380
Gly Gly Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu
385 390 395 400
Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln
405 410 415
Ser Val Leu Tyr Ser Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln
420 425 430
Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr
435 440 445
Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
450 455 460
Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val
465 470 475 480
Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly
485 490 495
Thr Lys Val Glu Ile Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly
500 505 510
Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val
515 520 525
Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser
530 535 540
Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val
545 550 555 560
Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro
565 570 575
Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr
580 585 590
Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser
595 600 605
Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly
610 615 620
Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val
625 630 635 640
Ser Ser Gly Gly Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro
645 650 655
Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
660 665 670
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
675 680 685
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
690 695 700
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
705 710 715 720
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
725 730 735
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
740 745 750
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
755 760 765
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
770 775 780
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
785 790 795 800
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
805 810 815
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
820 825 830
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
835 840 845
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
850 855 860
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly
865 870 875 880
Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His
885 890 895
Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn
900 905 910
Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro
915 920 925
Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys
930 935 940
Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr
945 950 955 960
Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu
965 970 975
Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln Lys
980 985 990
Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp Arg Val
995 1000 1005
Leu
<210> 20
<211> 1009
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 20
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Leu
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu
115 120 125
Leu Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn
130 135 140
Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln
145 150 155 160
Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala
165 170 175
Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr
180 185 190
Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile
195 200 205
Glu Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His
210 215 220
Leu His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr
225 230 235 240
Ile Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp
245 250 255
Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln
260 265 270
Lys Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp
275 280 285
Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly
290 295 300
Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp
305 310 315 320
Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu
325 330 335
Gly Lys Gly Asp Phe Arg Ile Leu Gly Gly Gly Gly Ser Gly Gly Gly
340 345 350
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
355 360 365
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
370 375 380
Gly Gly Gly Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu
385 390 395 400
Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln
405 410 415
Ser Val Leu Tyr Ser Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln
420 425 430
Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr
435 440 445
Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
450 455 460
Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val
465 470 475 480
Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly
485 490 495
Thr Lys Val Glu Ile Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly
500 505 510
Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val
515 520 525
Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser
530 535 540
Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val
545 550 555 560
Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro
565 570 575
Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr
580 585 590
Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser
595 600 605
Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly
610 615 620
Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val
625 630 635 640
Ser Ser Gly Gly Gly Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro
645 650 655
Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
660 665 670
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
675 680 685
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn
690 695 700
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
705 710 715 720
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
725 730 735
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
740 745 750
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
755 760 765
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp
770 775 780
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
785 790 795 800
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
805 810 815
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
820 825 830
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly
835 840 845
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
850 855 860
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly
865 870 875 880
Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe Lys Ile Gln His
885 890 895
Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met Lys Asn Ile Asn
900 905 910
Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro
915 920 925
Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys
930 935 940
Asn Gly Asp Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr
945 950 955 960
Met Cys Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu
965 970 975
Lys Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln Lys
980 985 990
Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp Arg Val
995 1000 1005
Leu
<210> 21
<211> 1316
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 21
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu
115 120 125
Leu Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn
130 135 140
Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln
145 150 155 160
Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala
165 170 175
Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr
180 185 190
Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile
195 200 205
Glu Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His
210 215 220
Leu His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr
225 230 235 240
Ile Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp
245 250 255
Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln
260 265 270
Lys Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp
275 280 285
Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly
290 295 300
Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp
305 310 315 320
Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu
325 330 335
Gly Lys Gly Asp Phe Arg Ile Leu Gly Gly Gly Gly Ser Gly Gly Gly
340 345 350
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
355 360 365
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
370 375 380
Gly Gly Gly Gly Ser Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val
385 390 395 400
Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr
405 410 415
Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys
420 425 430
Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg
435 440 445
Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser
450 455 460
Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr
465 470 475 480
Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp
485 490 495
Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Ser Gly
500 505 510
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly
515 520 525
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
530 535 540
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
545 550 555 560
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
565 570 575
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
580 585 590
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
595 600 605
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
610 615 620
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
625 630 635 640
Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
645 650 655
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
660 665 670
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala
675 680 685
Ala Gly Thr Val Phe Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu
690 695 700
Leu Thr Cys Ser Leu Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg
705 710 715 720
Trp Leu Lys Gly Gly Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln
725 730 735
Lys Thr Glu Phe Lys Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser
740 745 750
Cys Val Phe Leu Pro Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His
755 760 765
Gly Pro Pro Arg Val Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu
770 775 780
Gly Glu Thr Ala Met Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val
785 790 795 800
Thr Asp Trp Ala Trp Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu
805 810 815
Met Asn Gly Ser Glu Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg
820 825 830
Ser Glu Leu His Ile Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln
835 840 845
Tyr Arg Cys Asn Gly Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile
850 855 860
Thr Leu Arg Val Arg Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly
865 870 875 880
Ile Val Ala Glu Val Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu
885 890 895
Lys Arg Arg Lys Pro Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser
900 905 910
Ala Pro Leu Lys Ser Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn
915 920 925
Val Arg Gln Arg Asn Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly
930 935 940
Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
945 950 955 960
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
965 970 975
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
980 985 990
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
995 1000 1005
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
1010 1015 1020
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
1025 1030 1035
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
1040 1045 1050
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
1055 1060 1065
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
1070 1075 1080
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
1085 1090 1095
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
1100 1105 1110
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
1115 1120 1125
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
1130 1135 1140
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu
1145 1150 1155
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
1160 1165 1170
Gly Gly Ser Gly Gly Ser Leu His Val Lys Pro Pro Gln Phe Thr
1175 1180 1185
Trp Ala Gln Trp Phe Glu Thr Gln His Ile Asn Met Thr Ser Gln
1190 1195 1200
Gln Cys Thr Asn Ala Met Gln Val Ile Asn Asn Tyr Gln Arg Arg
1205 1210 1215
Cys Lys Asn Gln Asn Thr Phe Leu Leu Thr Thr Phe Ala Asn Val
1220 1225 1230
Val Asn Val Cys Gly Asn Pro Asn Met Thr Cys Pro Ser Asn Lys
1235 1240 1245
Thr Arg Lys Asn Cys His His Ser Gly Ser Gln Val Pro Leu Ile
1250 1255 1260
His Cys Asn Leu Thr Thr Pro Ser Pro Gln Asn Ile Ser Asn Cys
1265 1270 1275
Arg Tyr Ala Gln Thr Pro Ala Asn Met Phe Tyr Ile Val Ala Cys
1280 1285 1290
Asp Asn Arg Asp Gln Arg Arg Asp Pro Pro Gln Tyr Pro Val Val
1295 1300 1305
Pro Val His Leu Asp Arg Ile Ile
1310 1315
<210> 22
<211> 1316
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 22
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn Phe Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu
115 120 125
Leu Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn
130 135 140
Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln
145 150 155 160
Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala
165 170 175
Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr
180 185 190
Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile
195 200 205
Glu Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His
210 215 220
Leu His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr
225 230 235 240
Ile Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp
245 250 255
Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln
260 265 270
Lys Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp
275 280 285
Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly
290 295 300
Leu Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp
305 310 315 320
Pro Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu
325 330 335
Gly Lys Gly Asp Phe Arg Ile Leu Gly Gly Gly Gly Ser Gly Gly Gly
340 345 350
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
355 360 365
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
370 375 380
Gly Gly Gly Gly Ser Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val
385 390 395 400
Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr
405 410 415
Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys
420 425 430
Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg
435 440 445
Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser
450 455 460
Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr
465 470 475 480
Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp
485 490 495
Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Ser Gly
500 505 510
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly
515 520 525
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
530 535 540
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
545 550 555 560
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
565 570 575
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
580 585 590
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
595 600 605
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
610 615 620
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
625 630 635 640
Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
645 650 655
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
660 665 670
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala
675 680 685
Ala Gly Thr Val Phe Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu
690 695 700
Leu Thr Cys Ser Leu Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg
705 710 715 720
Trp Leu Lys Gly Gly Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln
725 730 735
Lys Thr Glu Phe Lys Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser
740 745 750
Cys Val Phe Leu Pro Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His
755 760 765
Gly Pro Pro Arg Val Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu
770 775 780
Gly Glu Thr Ala Met Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val
785 790 795 800
Thr Asp Trp Ala Trp Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu
805 810 815
Met Asn Gly Ser Glu Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg
820 825 830
Ser Glu Leu His Ile Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln
835 840 845
Tyr Arg Cys Asn Gly Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile
850 855 860
Thr Leu Arg Val Arg Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly
865 870 875 880
Ile Val Ala Glu Val Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu
885 890 895
Lys Arg Arg Lys Pro Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser
900 905 910
Ala Pro Leu Lys Ser Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn
915 920 925
Val Arg Gln Arg Asn Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly
930 935 940
Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu
945 950 955 960
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
965 970 975
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
980 985 990
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
995 1000 1005
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
1010 1015 1020
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
1025 1030 1035
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
1040 1045 1050
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
1055 1060 1065
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
1070 1075 1080
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
1085 1090 1095
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro
1100 1105 1110
Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
1115 1120 1125
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
1130 1135 1140
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu
1145 1150 1155
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
1160 1165 1170
Gly Gly Ser Gly Gly Ser Leu His Val Lys Pro Pro Gln Phe Thr
1175 1180 1185
Trp Ala Gln Trp Phe Glu Thr Gln His Ile Asn Met Thr Ser Gln
1190 1195 1200
Gln Cys Thr Asn Ala Met Gln Val Ile Asn Asn Tyr Gln Arg Arg
1205 1210 1215
Cys Lys Asn Gln Asn Thr Phe Leu Leu Thr Thr Phe Ala Asn Val
1220 1225 1230
Val Asn Val Cys Gly Asn Pro Asn Met Thr Cys Pro Ser Asn Lys
1235 1240 1245
Thr Arg Lys Asn Cys His His Ser Gly Ser Gln Val Pro Leu Ile
1250 1255 1260
His Cys Asn Leu Thr Thr Pro Ser Pro Gln Asn Ile Ser Asn Cys
1265 1270 1275
Arg Tyr Ala Gln Thr Pro Ala Asn Met Phe Tyr Ile Val Ala Cys
1280 1285 1290
Asp Asn Arg Asp Gln Arg Arg Asp Pro Pro Gln Tyr Pro Val Val
1295 1300 1305
Pro Val His Leu Asp Arg Ile Ile
1310 1315
<210> 23
<211> 779
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 23
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Leu
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
165 170 175
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
180 185 190
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
195 200 205
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
210 215 220
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
225 230 235 240
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
245 250 255
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
260 265 270
Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly
275 280 285
Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val Gln Ser Gly Thr Glu
290 295 300
Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly
305 310 315 320
Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly
325 330 335
Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr
340 345 350
Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
355 360 365
Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp
370 375 380
Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met
385 390 395 400
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
405 410 415
Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu
420 425 430
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
435 440 445
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
450 455 460
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
465 470 475 480
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
485 490 495
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
500 505 510
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
515 520 525
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
530 535 540
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
545 550 555 560
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
565 570 575
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
580 585 590
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
595 600 605
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
610 615 620
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
625 630 635 640
Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly Lys Pro Lys Gly Met
645 650 655
Thr Ser Ser Gln Trp Phe Lys Ile Gln His Met Gln Pro Ser Pro Gln
660 665 670
Ala Cys Asn Ser Ala Met Lys Asn Ile Asn Lys His Thr Lys Arg Cys
675 680 685
Lys Asp Leu Asn Thr Phe Leu His Glu Pro Phe Ser Ser Val Ala Ala
690 695 700
Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys Asn Gly Asp Lys Asn Cys
705 710 715 720
His Gln Ser His Gly Ala Val Ser Leu Thr Met Cys Lys Leu Thr Ser
725 730 735
Gly Lys His Pro Asn Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser
740 745 750
Tyr Val Val Ala Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe
755 760 765
His Leu Val Pro Val His Leu Asp Arg Val Leu
770 775
<210> 24
<211> 779
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 24
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
165 170 175
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
180 185 190
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
195 200 205
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
210 215 220
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
225 230 235 240
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
245 250 255
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
260 265 270
Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly
275 280 285
Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val Gln Ser Gly Thr Glu
290 295 300
Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly
305 310 315 320
Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly
325 330 335
Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr
340 345 350
Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
355 360 365
Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp
370 375 380
Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met
385 390 395 400
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
405 410 415
Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu
420 425 430
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
435 440 445
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
450 455 460
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
465 470 475 480
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
485 490 495
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
500 505 510
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
515 520 525
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
530 535 540
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
545 550 555 560
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
565 570 575
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
580 585 590
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
595 600 605
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
610 615 620
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
625 630 635 640
Ser Leu Ser Pro Gly Lys Gly Gly Ser Gly Gly Lys Pro Lys Gly Met
645 650 655
Thr Ser Ser Gln Trp Phe Lys Ile Gln His Met Gln Pro Ser Pro Gln
660 665 670
Ala Cys Asn Ser Ala Met Lys Asn Ile Asn Lys His Thr Lys Arg Cys
675 680 685
Lys Asp Leu Asn Thr Phe Leu His Glu Pro Phe Ser Ser Val Ala Ala
690 695 700
Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys Asn Gly Asp Lys Asn Cys
705 710 715 720
His Gln Ser His Gly Ala Val Ser Leu Thr Met Cys Lys Leu Thr Ser
725 730 735
Gly Lys His Pro Asn Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser
740 745 750
Tyr Val Val Ala Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe
755 760 765
His Leu Val Pro Val His Leu Asp Arg Val Leu
770 775
<210> 25
<211> 1077
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 25
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Leu
1 5 10 15
Phe Asn Phe Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
165 170 175
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
180 185 190
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
195 200 205
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
210 215 220
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
225 230 235 240
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
245 250 255
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
260 265 270
Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly
275 280 285
Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val Gln Ser Gly Thr Glu
290 295 300
Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly
305 310 315 320
Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly
325 330 335
Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr
340 345 350
Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
355 360 365
Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp
370 375 380
Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met
385 390 395 400
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
405 410 415
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
420 425 430
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
435 440 445
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ala Gly Thr Val Phe
450 455 460
Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu Leu Thr Cys Ser Leu
465 470 475 480
Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg Trp Leu Lys Gly Gly
485 490 495
Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln Lys Thr Glu Phe Lys
500 505 510
Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser Cys Val Phe Leu Pro
515 520 525
Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His Gly Pro Pro Arg Val
530 535 540
Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu Gly Glu Thr Ala Met
545 550 555 560
Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val Thr Asp Trp Ala Trp
565 570 575
Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu Met Asn Gly Ser Glu
580 585 590
Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg Ser Glu Leu His Ile
595 600 605
Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln Tyr Arg Cys Asn Gly
610 615 620
Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile Thr Leu Arg Val Arg
625 630 635 640
Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly Ile Val Ala Glu Val
645 650 655
Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu Lys Arg Arg Lys Pro
660 665 670
Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser Ala Pro Leu Lys Ser
675 680 685
Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn Val Arg Gln Arg Asn
690 695 700
Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Asp Lys Thr
705 710 715 720
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
725 730 735
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
740 745 750
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
755 760 765
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
770 775 780
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
785 790 795 800
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
805 810 815
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
820 825 830
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
835 840 845
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
850 855 860
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
865 870 875 880
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
885 890 895
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
900 905 910
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
915 920 925
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
930 935 940
Gly Gly Ser Gly Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe
945 950 955 960
Lys Ile Gln His Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met
965 970 975
Lys Asn Ile Asn Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe
980 985 990
Leu His Glu Pro Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys
995 1000 1005
Ile Ala Cys Lys Asn Gly Asp Lys Asn Cys His Gln Ser His Gly
1010 1015 1020
Ala Val Ser Leu Thr Met Cys Lys Leu Thr Ser Gly Lys His Pro
1025 1030 1035
Asn Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser Tyr Val Val
1040 1045 1050
Ala Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe His Leu
1055 1060 1065
Val Pro Val His Leu Asp Arg Val Leu
1070 1075
<210> 26
<211> 1077
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 26
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
165 170 175
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
180 185 190
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
195 200 205
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
210 215 220
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
225 230 235 240
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
245 250 255
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
260 265 270
Lys Arg Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly
275 280 285
Gly Ser Gly Gly Ser Gly Gln Met Gln Leu Val Gln Ser Gly Thr Glu
290 295 300
Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly
305 310 315 320
Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly
325 330 335
Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr
340 345 350
Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
355 360 365
Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp
370 375 380
Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met
385 390 395 400
Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly
405 410 415
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
420 425 430
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
435 440 445
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ala Gly Thr Val Phe
450 455 460
Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu Leu Thr Cys Ser Leu
465 470 475 480
Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg Trp Leu Lys Gly Gly
485 490 495
Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln Lys Thr Glu Phe Lys
500 505 510
Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser Cys Val Phe Leu Pro
515 520 525
Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His Gly Pro Pro Arg Val
530 535 540
Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu Gly Glu Thr Ala Met
545 550 555 560
Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val Thr Asp Trp Ala Trp
565 570 575
Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu Met Asn Gly Ser Glu
580 585 590
Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg Ser Glu Leu His Ile
595 600 605
Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln Tyr Arg Cys Asn Gly
610 615 620
Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile Thr Leu Arg Val Arg
625 630 635 640
Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly Ile Val Ala Glu Val
645 650 655
Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu Lys Arg Arg Lys Pro
660 665 670
Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser Ala Pro Leu Lys Ser
675 680 685
Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn Val Arg Gln Arg Asn
690 695 700
Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Asp Lys Thr
705 710 715 720
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
725 730 735
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
740 745 750
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
755 760 765
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
770 775 780
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
785 790 795 800
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
805 810 815
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
820 825 830
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
835 840 845
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
850 855 860
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
865 870 875 880
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
885 890 895
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
900 905 910
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
915 920 925
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
930 935 940
Gly Gly Ser Gly Gly Lys Pro Lys Gly Met Thr Ser Ser Gln Trp Phe
945 950 955 960
Lys Ile Gln His Met Gln Pro Ser Pro Gln Ala Cys Asn Ser Ala Met
965 970 975
Lys Asn Ile Asn Lys His Thr Lys Arg Cys Lys Asp Leu Asn Thr Phe
980 985 990
Leu His Glu Pro Phe Ser Ser Val Ala Ala Thr Cys Gln Thr Pro Lys
995 1000 1005
Ile Ala Cys Lys Asn Gly Asp Lys Asn Cys His Gln Ser His Gly
1010 1015 1020
Ala Val Ser Leu Thr Met Cys Lys Leu Thr Ser Gly Lys His Pro
1025 1030 1035
Asn Cys Arg Tyr Lys Glu Lys Arg Gln Asn Lys Ser Tyr Val Val
1040 1045 1050
Ala Cys Lys Pro Pro Gln Lys Lys Asp Ser Gln Gln Phe His Leu
1055 1060 1065
Val Pro Val His Leu Asp Arg Val Leu
1070 1075
<210> 27
<211> 1113
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 27
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn Phe Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
165 170 175
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
180 185 190
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
195 200 205
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
210 215 220
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
225 230 235 240
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
245 250 255
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
260 265 270
Thr Thr Val Thr Val Ser Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly
275 280 285
Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly Asp Ile Gln Leu Thr
290 295 300
Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile
305 310 315 320
Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Ile Asn Lys Asn
325 330 335
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu
340 345 350
Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser
355 360 365
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
370 375 380
Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro
385 390 395 400
Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly
405 410 415
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
420 425 430
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
435 440 445
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ala Gly Thr Val Phe
450 455 460
Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu Leu Thr Cys Ser Leu
465 470 475 480
Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg Trp Leu Lys Gly Gly
485 490 495
Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln Lys Thr Glu Phe Lys
500 505 510
Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser Cys Val Phe Leu Pro
515 520 525
Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His Gly Pro Pro Arg Val
530 535 540
Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu Gly Glu Thr Ala Met
545 550 555 560
Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val Thr Asp Trp Ala Trp
565 570 575
Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu Met Asn Gly Ser Glu
580 585 590
Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg Ser Glu Leu His Ile
595 600 605
Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln Tyr Arg Cys Asn Gly
610 615 620
Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile Thr Leu Arg Val Arg
625 630 635 640
Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly Ile Val Ala Glu Val
645 650 655
Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu Lys Arg Arg Lys Pro
660 665 670
Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser Ala Pro Leu Lys Ser
675 680 685
Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn Val Arg Gln Arg Asn
690 695 700
Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Asp Lys Thr
705 710 715 720
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
725 730 735
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
740 745 750
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
755 760 765
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
770 775 780
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
785 790 795 800
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
805 810 815
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
820 825 830
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
835 840 845
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
850 855 860
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
865 870 875 880
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
885 890 895
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
900 905 910
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
915 920 925
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
930 935 940
Gly Gly Ser Gly Gly Met Arg Ala Tyr Trp Pro Asp Val Ile His Ser
945 950 955 960
Phe Pro Asn Arg Ser Arg Phe Trp Lys His Glu Trp Glu Lys His Gly
965 970 975
Thr Cys Ala Ala Gln Val Asp Ala Leu Asn Ser Gln Lys Lys Tyr Phe
980 985 990
Gly Arg Ser Leu Glu Leu Tyr Arg Glu Leu Asp Leu Asn Ser Val Leu
995 1000 1005
Leu Lys Leu Gly Ile Lys Pro Ser Ile Asn Tyr Tyr Gln Val Ala
1010 1015 1020
Asp Phe Lys Asp Ala Leu Ala Arg Val Tyr Gly Val Ile Pro Lys
1025 1030 1035
Ile Gln Cys Leu Pro Pro Ser Gln Asp Glu Glu Val Gln Thr Ile
1040 1045 1050
Gly Gln Ile Glu Leu Cys Leu Thr Lys Gln Asp Gln Gln Leu Gln
1055 1060 1065
Asn Cys Thr Glu Pro Gly Glu Gln Pro Ser Pro Lys Gln Glu Val
1070 1075 1080
Trp Leu Ala Asn Gly Ala Ala Glu Ser Arg Gly Leu Arg Val Cys
1085 1090 1095
Glu Asp Gly Pro Val Phe Tyr Pro Pro Pro Lys Lys Thr Lys His
1100 1105 1110
<210> 28
<211> 1113
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 28
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys
1 5 10 15
Phe Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
130 135 140
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
145 150 155 160
Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro Gly Glu
165 170 175
Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile Thr Tyr
180 185 190
Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met
195 200 205
Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro Ser Phe
210 215 220
Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr Ala Tyr
225 230 235 240
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr Tyr Cys
245 250 255
Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly Gln Gly
260 265 270
Thr Thr Val Thr Val Ser Ser Gly Gly Ser Gly Gly Gly Gly Ser Gly
275 280 285
Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly Asp Ile Gln Leu Thr
290 295 300
Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile
305 310 315 320
Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Ile Asn Lys Asn
325 330 335
Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu
340 345 350
Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser
355 360 365
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
370 375 380
Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro
385 390 395 400
Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly
405 410 415
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
420 425 430
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
435 440 445
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ala Gly Thr Val Phe
450 455 460
Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu Leu Thr Cys Ser Leu
465 470 475 480
Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg Trp Leu Lys Gly Gly
485 490 495
Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln Lys Thr Glu Phe Lys
500 505 510
Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser Cys Val Phe Leu Pro
515 520 525
Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His Gly Pro Pro Arg Val
530 535 540
Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu Gly Glu Thr Ala Met
545 550 555 560
Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val Thr Asp Trp Ala Trp
565 570 575
Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu Met Asn Gly Ser Glu
580 585 590
Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg Ser Glu Leu His Ile
595 600 605
Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln Tyr Arg Cys Asn Gly
610 615 620
Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile Thr Leu Arg Val Arg
625 630 635 640
Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly Ile Val Ala Glu Val
645 650 655
Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu Lys Arg Arg Lys Pro
660 665 670
Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser Ala Pro Leu Lys Ser
675 680 685
Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn Val Arg Gln Arg Asn
690 695 700
Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Asp Lys Thr
705 710 715 720
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
725 730 735
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
740 745 750
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
755 760 765
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
770 775 780
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
785 790 795 800
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
805 810 815
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
820 825 830
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
835 840 845
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
850 855 860
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
865 870 875 880
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
885 890 895
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
900 905 910
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
915 920 925
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
930 935 940
Gly Gly Ser Gly Gly Met Arg Ala Tyr Trp Pro Asp Val Ile His Ser
945 950 955 960
Phe Pro Asn Arg Ser Arg Phe Trp Lys His Glu Trp Glu Lys His Gly
965 970 975
Thr Cys Ala Ala Gln Val Asp Ala Leu Asn Ser Gln Lys Lys Tyr Phe
980 985 990
Gly Arg Ser Leu Glu Leu Tyr Arg Glu Leu Asp Leu Asn Ser Val Leu
995 1000 1005
Leu Lys Leu Gly Ile Lys Pro Ser Ile Asn Tyr Tyr Gln Val Ala
1010 1015 1020
Asp Phe Lys Asp Ala Leu Ala Arg Val Tyr Gly Val Ile Pro Lys
1025 1030 1035
Ile Gln Cys Leu Pro Pro Ser Gln Asp Glu Glu Val Gln Thr Ile
1040 1045 1050
Gly Gln Ile Glu Leu Cys Leu Thr Lys Gln Asp Gln Gln Leu Gln
1055 1060 1065
Asn Cys Thr Glu Pro Gly Glu Gln Pro Ser Pro Lys Gln Glu Val
1070 1075 1080
Trp Leu Ala Asn Gly Ala Ala Glu Ser Arg Gly Leu Arg Val Cys
1085 1090 1095
Glu Asp Gly Pro Val Phe Tyr Pro Pro Pro Lys Lys Thr Lys His
1100 1105 1110
<210> 29
<211> 988
<212> PRT
<213> Artificial
<220>
<223> fuiosn protein
<400> 29
Met Gly Ser Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Leu
1 5 10 15
Phe Asn Phe Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser
20 25 30
Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn
35 40 45
Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu
50 55 60
Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu
65 70 75 80
Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp
85 90 95
Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr
100 105 110
Ser Thr Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gln Met Gln Leu Val Gln Ser Gly Thr Glu Val Lys Lys Pro
130 135 140
Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Gly Phe Ile
145 150 155 160
Thr Tyr Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu
165 170 175
Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser Glu Thr Arg Tyr Ser Pro
180 185 190
Ser Phe Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Asn Thr
195 200 205
Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Ile Tyr
210 215 220
Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr Pro Met Asp Val Trp Gly
225 230 235 240
Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Ser Gly Gly Gly Gly
245 250 255
Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly Asp Ile Gln
260 265 270
Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala
275 280 285
Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Ile Asn
290 295 300
Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys
305 310 315 320
Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg
325 330 335
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser
340 345 350
Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser
355 360 365
Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly
370 375 380
Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ala Ala
385 390 395 400
Gly Thr Val Phe Thr Thr Val Glu Asp Leu Gly Ser Lys Ile Leu Leu
405 410 415
Thr Cys Ser Leu Asn Asp Ser Ala Thr Glu Val Thr Gly His Arg Trp
420 425 430
Leu Lys Gly Gly Val Val Leu Lys Glu Asp Ala Leu Pro Gly Gln Lys
435 440 445
Thr Glu Phe Lys Val Asp Ser Asp Asp Gln Trp Gly Glu Tyr Ser Cys
450 455 460
Val Phe Leu Pro Glu Pro Met Gly Thr Ala Asn Ile Gln Leu His Gly
465 470 475 480
Pro Pro Arg Val Lys Ala Val Lys Ser Ser Glu His Ile Asn Glu Gly
485 490 495
Glu Thr Ala Met Leu Val Cys Lys Ser Glu Ser Val Pro Pro Val Thr
500 505 510
Asp Trp Ala Trp Tyr Lys Ile Thr Asp Ser Glu Asp Lys Ala Leu Met
515 520 525
Asn Gly Ser Glu Ser Arg Phe Phe Val Ser Ser Ser Gln Gly Arg Ser
530 535 540
Glu Leu His Ile Glu Asn Leu Asn Met Glu Ala Asp Pro Gly Gln Tyr
545 550 555 560
Arg Cys Asn Gly Thr Ser Ser Lys Gly Ser Asp Gln Ala Ile Ile Thr
565 570 575
Leu Arg Val Arg Ser His Leu Ala Ala Leu Trp Pro Leu Leu Gly Ile
580 585 590
Val Ala Glu Val Leu Val Leu Val Thr Ile Ile Phe Ile Tyr Glu Lys
595 600 605
Arg Arg Lys Pro Glu Asp Val Leu Asp Asp Asp Asp Ala Gly Ser Ala
610 615 620
Pro Leu Lys Ser Ser Gly Gln His Gln Asn Asp Lys Gly Lys Asn Val
625 630 635 640
Arg Gln Arg Asn Ser Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly
645 650 655
Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu
660 665 670
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
675 680 685
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
690 695 700
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
705 710 715 720
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
725 730 735
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
740 745 750
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
755 760 765
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
770 775 780
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
785 790 795 800
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
805 810 815
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
820 825 830
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
835 840 845
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
850 855 860
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
865 870 875 880
Ser Pro Gly Lys Gly Gly Ser Gly Gly Gly Met Ala Ser Leu Leu Cys
885 890 895
Cys Gly Pro Lys Leu Ala Ala Cys Gly Ile Val Leu Ser Ala Trp Gly
900 905 910
Val Ile Met Leu Ile Met Leu Gly Ile Phe Phe Asn Val His Ser Ala
915 920 925
Val Leu Ile Glu Asp Val Pro Phe Thr Glu Lys Asp Phe Glu Asn Gly
930 935 940
Pro Gln Asn Ile Tyr Asn Leu Tyr Glu Gln Val Ser Tyr Asn Cys Phe
945 950 955 960
Ile Ala Ala Gly Leu Tyr Leu Leu Leu Gly Gly Phe Ser Phe Cys Gln
965 970 975
Val Arg Leu Asn Lys Arg Lys Glu Tyr Met Val Arg
980 985
<210> 30
<211> 1028
<212> PRT
<213> Homo sapiens
<400> 30
Met Gly Ser Ala Ala Cys Pro Arg Gly Ala Leu Pro Glu Leu Ala Pro
1 5 10 15
Cys Cys Gln Pro Arg Glu Gln Ser Gln Pro His Thr Arg Trp Asp Ala
20 25 30
Gly Cys Gly Ile Gln His Pro Gly Gly Glu Glu Phe Arg Thr Leu Gly
35 40 45
Gly Ala Arg Ala Tyr Arg Val Pro Asn Ser Gln Glu Gly Arg Ser Ser
50 55 60
Pro Thr Arg Phe Phe Pro Ala Pro Glu Gly Pro Ala His Cys Phe Val
65 70 75 80
Ser Ser Pro Asp Arg Ala Phe Trp Val Ser Glu Glu Val Gln Arg Leu
85 90 95
Leu Leu Ser Asn Ala Cys Gln Pro Lys Glu Cys Asn Gly Val Lys Ile
100 105 110
Pro Val Asp Ala Ser Lys Pro Asn Pro Asn Asp Val Glu Phe Asp Asn
115 120 125
Leu Tyr Leu Asp Met Asn Gly Ile Ile His Pro Cys Thr His Pro Glu
130 135 140
Asp Lys Pro Ala Pro Lys Asn Glu Asp Glu Met Met Val Ala Ile Phe
145 150 155 160
Glu Tyr Ile Asp Arg Leu Phe Ser Ile Val Arg Pro Arg Arg Leu Leu
165 170 175
Tyr Met Ala Ile Asp Gly Val Ala Pro Arg Ala Lys Met Asn Gln Gln
180 185 190
Arg Ser Arg Arg Phe Arg Ala Ser Lys Glu Gly Met Glu Ala Ala Val
195 200 205
Glu Lys Gln Arg Val Arg Glu Glu Ile Leu Ala Lys Gly Gly Phe Leu
210 215 220
Pro Pro Glu Glu Ile Lys Glu Arg Phe Asp Ser Asn Cys Ile Thr Pro
225 230 235 240
Gly Thr Glu Phe Met Asp Asn Leu Ala Lys Cys Leu Arg Tyr Tyr Ile
245 250 255
Ala Asp Arg Leu Asn Asn Asp Pro Gly Trp Lys Asn Leu Thr Val Ile
260 265 270
Leu Ser Asp Ala Ser Ala Pro Gly Glu Gly Glu His Lys Ile Met Asp
275 280 285
Tyr Ile Arg Arg Gln Arg Ala Gln Pro Asn His Asp Pro Asn Thr His
290 295 300
His Cys Leu Cys Gly Ala Asp Ala Asp Leu Ile Met Leu Gly Leu Ala
305 310 315 320
Thr His Glu Pro Asn Phe Thr Ile Ile Arg Glu Glu Phe Lys Pro Asn
325 330 335
Lys Pro Lys Pro Cys Gly Leu Cys Asn Gln Phe Gly His Glu Val Lys
340 345 350
Asp Cys Glu Gly Leu Pro Arg Glu Lys Lys Gly Lys His Asp Glu Leu
355 360 365
Ala Asp Ser Leu Pro Cys Ala Glu Gly Glu Phe Ile Phe Leu Arg Leu
370 375 380
Asn Val Leu Arg Glu Tyr Leu Glu Arg Glu Leu Thr Met Ala Ser Leu
385 390 395 400
Pro Phe Thr Phe Asp Val Glu Arg Ser Ile Asp Asp Trp Val Phe Met
405 410 415
Cys Phe Phe Val Gly Asn Asp Phe Leu Pro His Leu Pro Ser Leu Glu
420 425 430
Ile Arg Glu Asn Ala Ile Asp Arg Leu Val Asn Ile Tyr Lys Asn Val
435 440 445
Val His Lys Thr Gly Gly Tyr Leu Thr Glu Ser Gly Tyr Val Asn Leu
450 455 460
Gln Arg Val Gln Met Ile Met Leu Ala Val Gly Glu Val Glu Asp Ser
465 470 475 480
Ile Phe Lys Lys Arg Lys Asp Asp Glu Asp Ser Phe Arg Arg Arg Gln
485 490 495
Lys Glu Lys Arg Lys Arg Met Lys Arg Asp Gln Pro Ala Phe Thr Pro
500 505 510
Ser Gly Ile Leu Thr Pro His Ala Leu Gly Ser Arg Asn Ser Pro Gly
515 520 525
Ser Gln Val Ala Ser Asn Pro Arg Gln Ala Ala Tyr Glu Met Arg Met
530 535 540
Gln Asn Asn Ser Ser Pro Ser Ile Ser Pro Asn Thr Ser Phe Thr Ser
545 550 555 560
Asp Gly Ser Pro Ser Pro Leu Gly Gly Ile Lys Arg Lys Ala Glu Asp
565 570 575
Ser Asp Ser Glu Pro Glu Pro Glu Asp Asn Val Arg Leu Trp Glu Ala
580 585 590
Gly Trp Lys Gln Arg Tyr Tyr Lys Asn Lys Phe Asp Val Asp Ala Ala
595 600 605
Asp Glu Lys Phe Arg Arg Lys Val Val Gln Ser Tyr Val Glu Gly Leu
610 615 620
Cys Trp Val Leu Arg Tyr Tyr Tyr Gln Gly Cys Ala Ser Trp Lys Trp
625 630 635 640
Tyr Tyr Pro Phe His Tyr Ala Pro Phe Ala Ser Asp Phe Glu Gly Ile
645 650 655
Ala Asp Met Pro Ser Asp Phe Glu Lys Gly Thr Lys Pro Phe Lys Pro
660 665 670
Leu Glu Gln Leu Met Gly Val Phe Pro Ala Ala Ser Gly Asn Phe Leu
675 680 685
Pro Pro Ser Trp Arg Lys Leu Met Ser Asp Pro Asp Ser Ser Ile Ile
690 695 700
Asp Phe Tyr Pro Glu Asp Phe Ala Ile Asp Leu Asn Gly Lys Lys Tyr
705 710 715 720
Ala Trp Gln Gly Val Ala Leu Leu Pro Phe Val Asp Glu Arg Arg Leu
725 730 735
Arg Ala Ala Leu Glu Glu Val Tyr Pro Asp Leu Thr Pro Glu Glu Thr
740 745 750
Arg Arg Asn Ser Leu Gly Gly Asp Val Leu Phe Val Gly Lys His His
755 760 765
Pro Leu His Asp Phe Ile Leu Glu Leu Tyr Gln Thr Gly Ser Thr Glu
770 775 780
Pro Val Glu Val Pro Pro Glu Leu Cys His Gly Ile Gln Gly Lys Phe
785 790 795 800
Ser Leu Asp Glu Glu Ala Ile Leu Pro Asp Gln Ile Val Cys Ser Pro
805 810 815
Val Pro Met Leu Arg Asp Leu Thr Gln Asn Thr Val Val Ser Ile Asn
820 825 830
Phe Lys Asp Pro Gln Phe Ala Glu Asp Tyr Ile Phe Lys Ala Val Met
835 840 845
Leu Pro Gly Ala Arg Lys Pro Ala Ala Val Leu Lys Pro Ser Asp Trp
850 855 860
Glu Lys Ser Ser Asn Gly Arg Gln Trp Lys Pro Gln Leu Gly Phe Asn
865 870 875 880
Arg Asp Arg Arg Pro Val His Leu Asp Gln Ala Ala Phe Arg Thr Leu
885 890 895
Gly His Val Met Pro Arg Gly Ser Gly Thr Gly Ile Tyr Ser Asn Ala
900 905 910
Ala Pro Pro Pro Val Thr Tyr Gln Gly Asn Leu Tyr Arg Pro Leu Leu
915 920 925
Arg Gly Gln Ala Gln Ile Pro Lys Leu Met Ser Asn Met Arg Pro Gln
930 935 940
Asp Ser Trp Arg Gly Pro Pro Pro Leu Phe Gln Gln Gln Arg Phe Asp
945 950 955 960
Arg Gly Val Gly Ala Glu Pro Leu Leu Pro Trp Asn Arg Met Leu Gln
965 970 975
Thr Gln Asn Ala Ala Phe Gln Pro Asn Gln Tyr Gln Met Leu Ala Gly
980 985 990
Pro Gly Gly Tyr Pro Pro Arg Arg Asp Asp Arg Gly Gly Arg Gln Gly
995 1000 1005
Tyr Pro Arg Glu Gly Arg Lys Tyr Pro Leu Pro Pro Pro Ser Gly
1010 1015 1020
Arg Tyr Asn Trp Asn
1025
<210> 31
<211> 1694
<212> PRT
<213> Homo sapiens
<400> 31
Met Gly Val Pro Lys Phe Tyr Arg Trp Ile Ser Glu Arg Tyr Pro Cys
1 5 10 15
Leu Ser Glu Val Val Lys Glu His Gln Ile Pro Glu Phe Asp Asn Leu
20 25 30
Tyr Leu Asp Met Asn Gly Ile Ile His Gln Cys Ser His Pro Asn Asp
35 40 45
Asp Asp Val His Phe Arg Ile Ser Asp Asp Lys Ile Phe Thr Asp Ile
50 55 60
Phe His Tyr Leu Glu Val Leu Phe Arg Ile Ile Lys Pro Arg Lys Val
65 70 75 80
Phe Phe Met Ala Val Asp Gly Val Ala Pro Arg Ala Lys Met Asn Gln
85 90 95
Gln Arg Gly Arg Arg Phe Arg Ser Ala Lys Glu Ala Glu Asp Lys Ile
100 105 110
Lys Lys Ala Ile Glu Lys Gly Glu Thr Leu Pro Thr Glu Ala Arg Phe
115 120 125
Asp Ser Asn Cys Ile Thr Pro Gly Thr Glu Phe Met Ala Arg Leu His
130 135 140
Glu His Leu Lys Tyr Phe Val Asn Met Lys Ile Ser Thr Asp Lys Ser
145 150 155 160
Trp Gln Gly Val Thr Ile Tyr Phe Ser Gly His Glu Thr Pro Gly Glu
165 170 175
Gly Glu His Lys Ile Met Glu Phe Ile Arg Ser Glu Lys Ala Lys Pro
180 185 190
Asp His Asp Pro Asn Thr Arg His Cys Leu Tyr Gly Leu Asp Ala Asp
195 200 205
Leu Ile Met Leu Gly Leu Thr Ser His Glu Ala His Phe Ser Leu Leu
210 215 220
Arg Glu Glu Val Arg Phe Gly Gly Lys Lys Thr Gln Arg Val Cys Ala
225 230 235 240
Pro Glu Glu Thr Thr Phe His Leu Leu His Leu Ser Leu Met Arg Glu
245 250 255
Tyr Ile Asp Tyr Glu Phe Ser Val Leu Lys Glu Lys Ile Thr Phe Lys
260 265 270
Tyr Asp Ile Glu Arg Ile Ile Asp Asp Trp Ile Leu Met Gly Phe Leu
275 280 285
Val Gly Asn Asp Phe Ile Pro His Leu Pro His Leu His Ile Asn His
290 295 300
Asp Ala Leu Pro Leu Leu Tyr Gly Thr Tyr Val Thr Ile Leu Pro Glu
305 310 315 320
Leu Gly Gly Tyr Ile Asn Glu Ser Gly His Leu Asn Leu Pro Arg Phe
325 330 335
Glu Lys Tyr Leu Val Lys Leu Ser Asp Phe Asp Arg Glu His Phe Ser
340 345 350
Glu Val Phe Val Asp Leu Lys Trp Phe Glu Ser Lys Val Gly Asn Lys
355 360 365
Tyr Leu Asn Glu Ala Ala Gly Val Ala Ala Glu Glu Ala Arg Asn Tyr
370 375 380
Lys Glu Lys Lys Lys Leu Lys Gly Gln Glu Asn Ser Leu Cys Trp Thr
385 390 395 400
Ala Leu Asp Lys Asn Glu Gly Glu Met Ile Thr Ser Lys Asp Asn Leu
405 410 415
Glu Asp Glu Thr Glu Asp Asp Asp Leu Phe Glu Thr Glu Phe Arg Gln
420 425 430
Tyr Lys Arg Thr Tyr Tyr Met Thr Lys Met Gly Val Asp Val Val Ser
435 440 445
Asp Asp Phe Leu Ala Asp Gln Ala Ala Cys Tyr Val Gln Ala Ile Gln
450 455 460
Trp Ile Leu His Tyr Tyr Tyr His Gly Val Gln Ser Trp Ser Trp Tyr
465 470 475 480
Tyr Pro Tyr His Tyr Ala Pro Phe Leu Ser Asp Ile His Asn Ile Ser
485 490 495
Thr Leu Lys Ile His Phe Glu Leu Gly Lys Pro Phe Lys Pro Phe Glu
500 505 510
Gln Leu Leu Ala Val Leu Pro Ala Ala Ser Lys Asn Leu Leu Pro Ala
515 520 525
Cys Tyr Gln His Leu Met Thr Asn Glu Asp Ser Pro Ile Ile Glu Tyr
530 535 540
Tyr Pro Pro Asp Phe Lys Thr Asp Leu Asn Gly Lys Gln Gln Glu Trp
545 550 555 560
Glu Ala Val Val Leu Ile Pro Phe Ile Asp Glu Lys Arg Leu Leu Glu
565 570 575
Ala Met Glu Thr Cys Asn His Ser Leu Lys Lys Glu Glu Arg Lys Arg
580 585 590
Asn Gln His Ser Glu Cys Leu Met Cys Trp Tyr Asp Arg Asp Thr Glu
595 600 605
Phe Ile Tyr Pro Ser Pro Trp Pro Glu Lys Phe Pro Ala Ile Glu Arg
610 615 620
Cys Cys Thr Arg Tyr Lys Ile Ile Ser Leu Asp Ala Trp Arg Val Asp
625 630 635 640
Ile Asn Lys Asn Lys Ile Thr Arg Ile Asp Gln Lys Ala Leu Tyr Phe
645 650 655
Cys Gly Phe Pro Thr Leu Lys His Ile Arg His Lys Phe Phe Leu Lys
660 665 670
Lys Ser Gly Val Gln Val Phe Gln Gln Ser Ser Arg Gly Glu Asn Met
675 680 685
Met Leu Glu Ile Leu Val Asp Ala Glu Ser Asp Glu Leu Thr Val Glu
690 695 700
Asn Val Ala Ser Ser Val Leu Gly Lys Ser Val Phe Val Asn Trp Pro
705 710 715 720
His Leu Glu Glu Ala Arg Val Val Ala Val Ser Asp Gly Glu Thr Lys
725 730 735
Phe Tyr Leu Glu Glu Pro Pro Gly Thr Gln Lys Leu Tyr Ser Gly Arg
740 745 750
Thr Ala Pro Pro Ser Lys Val Val His Leu Gly Asp Lys Glu Gln Ser
755 760 765
Asn Trp Ala Lys Glu Val Gln Gly Ile Ser Glu His Tyr Leu Arg Arg
770 775 780
Lys Gly Ile Ile Ile Asn Glu Thr Ser Ala Val Val Tyr Ala Gln Leu
785 790 795 800
Leu Thr Gly Arg Lys Tyr Gln Ile Asn Gln Asn Gly Glu Val Arg Leu
805 810 815
Glu Lys Gln Trp Ser Lys Gln Val Val Pro Phe Val Tyr Gln Thr Ile
820 825 830
Val Lys Asp Ile Arg Ala Phe Asp Ser Arg Phe Ser Asn Ile Lys Thr
835 840 845
Leu Asp Asp Leu Phe Pro Leu Arg Ser Met Val Phe Met Leu Gly Thr
850 855 860
Pro Tyr Tyr Gly Cys Thr Gly Glu Val Gln Asp Ser Gly Asp Val Ile
865 870 875 880
Thr Glu Gly Arg Ile Arg Val Ile Phe Ser Ile Pro Cys Glu Pro Asn
885 890 895
Leu Asp Ala Leu Ile Gln Asn Gln His Lys Tyr Ser Ile Lys Tyr Asn
900 905 910
Pro Gly Tyr Val Leu Ala Ser Arg Leu Gly Val Ser Gly Tyr Leu Val
915 920 925
Ser Arg Phe Thr Gly Ser Ile Phe Ile Gly Arg Gly Ser Arg Arg Asn
930 935 940
Pro His Gly Asp His Lys Ala Asn Val Gly Leu Asn Leu Lys Phe Asn
945 950 955 960
Lys Lys Asn Glu Glu Val Pro Gly Tyr Thr Lys Lys Val Gly Ser Glu
965 970 975
Trp Met Tyr Ser Ser Ala Ala Glu Gln Leu Leu Ala Glu Tyr Leu Glu
980 985 990
Arg Ala Pro Glu Leu Phe Ser Tyr Ile Ala Lys Asn Ser Gln Glu Asp
995 1000 1005
Val Phe Tyr Glu Asp Asp Ile Trp Pro Gly Glu Asn Glu Asn Gly
1010 1015 1020
Ala Glu Lys Val Gln Glu Ile Ile Thr Trp Leu Lys Gly His Pro
1025 1030 1035
Val Ser Thr Leu Ser Arg Ser Ser Cys Asp Leu Gln Ile Leu Asp
1040 1045 1050
Ala Ala Ile Val Glu Lys Ile Glu Glu Glu Val Glu Lys Cys Lys
1055 1060 1065
Gln Arg Lys Asn Asn Lys Lys Val Arg Val Thr Val Lys Pro His
1070 1075 1080
Leu Leu Tyr Arg Pro Leu Glu Gln Gln His Gly Val Ile Pro Asp
1085 1090 1095
Arg Asp Ala Glu Phe Cys Leu Phe Asp Arg Val Val Asn Val Arg
1100 1105 1110
Glu Asn Phe Ser Val Pro Val Gly Leu Arg Gly Thr Ile Ile Gly
1115 1120 1125
Ile Lys Gly Ala Asn Arg Glu Ala Asp Val Leu Phe Glu Val Leu
1130 1135 1140
Phe Asp Glu Glu Phe Pro Gly Gly Leu Thr Ile Arg Cys Ser Pro
1145 1150 1155
Gly Arg Gly Tyr Arg Leu Pro Thr Ser Ala Leu Val Asn Leu Ser
1160 1165 1170
His Gly Ser Arg Ser Glu Thr Gly Asn Gln Lys Leu Thr Ala Ile
1175 1180 1185
Val Lys Pro Gln Pro Ala Val His Gln His Ser Ser Ser Ser Ser
1190 1195 1200
Val Ser Ser Gly His Leu Gly Ala Leu Asn His Ser Pro Gln Ser
1205 1210 1215
Leu Phe Val Pro Thr Gln Val Pro Thr Lys Asp Asp Asp Glu Phe
1220 1225 1230
Cys Asn Ile Trp Gln Ser Leu Gln Gly Ser Gly Lys Met Gln Tyr
1235 1240 1245
Phe Gln Pro Thr Ile Gln Glu Lys Gly Ala Val Leu Pro Gln Glu
1250 1255 1260
Ile Ser Gln Val Asn Gln His His Lys Ser Gly Phe Asn Asp Asn
1265 1270 1275
Ser Val Lys Tyr Gln Gln Arg Lys His Asp Pro His Arg Lys Phe
1280 1285 1290
Lys Glu Glu Cys Lys Ser Pro Lys Ala Glu Cys Trp Ser Gln Lys
1295 1300 1305
Met Ser Asn Lys Gln Pro Asn Ser Gly Ile Glu Asn Phe Leu Ala
1310 1315 1320
Ser Leu Asn Ile Ser Lys Glu Asn Glu Val Gln Ser Ser His His
1325 1330 1335
Gly Glu Pro Pro Ser Glu Glu His Leu Ser Pro Gln Ser Phe Ala
1340 1345 1350
Met Lys Gly Thr Arg Met Leu Lys Glu Ile Leu Lys Ile Asp Gly
1355 1360 1365
Ser Asn Thr Val Asp His Lys Asn Glu Ile Lys Gln Ile Ala Asn
1370 1375 1380
Glu Ile Pro Val Ser Ser Asn Arg Arg Asp Glu Tyr Gly Leu Pro
1385 1390 1395
Ser Gln Pro Lys Gln Asn Lys Lys Leu Ala Ser Tyr Met Asn Lys
1400 1405 1410
Pro His Ser Ala Asn Glu Tyr His Asn Val Gln Ser Met Asp Asn
1415 1420 1425
Met Cys Trp Pro Ala Pro Ser Gln Ile Pro Pro Val Ser Thr Pro
1430 1435 1440
Val Thr Glu Leu Ser Arg Ile Cys Ser Leu Val Gly Met Pro Gln
1445 1450 1455
Pro Asp Phe Ser Phe Leu Arg Met Pro Gln Thr Met Thr Val Cys
1460 1465 1470
Gln Val Lys Leu Ser Asn Gly Leu Leu Val His Gly Pro Gln Cys
1475 1480 1485
His Ser Glu Asn Glu Ala Lys Glu Lys Ala Ala Leu Phe Ala Leu
1490 1495 1500
Gln Gln Leu Gly Ser Leu Gly Met Asn Phe Pro Leu Pro Ser Gln
1505 1510 1515
Val Phe Ala Asn Tyr Pro Ser Ala Val Pro Pro Gly Thr Ile Pro
1520 1525 1530
Pro Ala Phe Pro Pro Pro Thr Ala Asn Ile Met Pro Ser Ser Ser
1535 1540 1545
His Leu Phe Gly Ser Met Pro Trp Gly Pro Ser Val Pro Val Pro
1550 1555 1560
Gly Lys Pro Phe His His Thr Leu Tyr Ser Gly Thr Met Pro Met
1565 1570 1575
Ala Gly Gly Ile Pro Gly Gly Val His Asn Gln Phe Ile Pro Leu
1580 1585 1590
Gln Val Thr Lys Lys Arg Val Ala Asn Lys Lys Asn Phe Glu Asn
1595 1600 1605
Lys Glu Ala Gln Ser Ser Gln Ala Thr Pro Val Gln Thr Ser Gln
1610 1615 1620
Pro Asp Ser Ser Asn Ile Val Lys Val Ser Pro Arg Glu Ser Ser
1625 1630 1635
Ser Ala Ser Leu Lys Ser Ser Pro Ile Ala Gln Pro Ala Ser Ser
1640 1645 1650
Phe Gln Val Glu Thr Ala Ser Gln Gly His Ser Ile Ser His His
1655 1660 1665
Lys Ser Thr Pro Ile Ser Ser Ser Arg Arg Lys Ser Arg Lys Leu
1670 1675 1680
Ala Val Asn Phe Gly Val Ser Lys Pro Ser Glu
1685 1690
<210> 32
<211> 269
<212> PRT
<213> Homo sapiens
<400> 32
Met Ala Ala Ala Leu Phe Val Leu Leu Gly Phe Ala Leu Leu Gly Thr
1 5 10 15
His Gly Ala Ser Gly Ala Ala Gly Thr Val Phe Thr Thr Val Glu Asp
20 25 30
Leu Gly Ser Lys Ile Leu Leu Thr Cys Ser Leu Asn Asp Ser Ala Thr
35 40 45
Glu Val Thr Gly His Arg Trp Leu Lys Gly Gly Val Val Leu Lys Glu
50 55 60
Asp Ala Leu Pro Gly Gln Lys Thr Glu Phe Lys Val Asp Ser Asp Asp
65 70 75 80
Gln Trp Gly Glu Tyr Ser Cys Val Phe Leu Pro Glu Pro Met Gly Thr
85 90 95
Ala Asn Ile Gln Leu His Gly Pro Pro Arg Val Lys Ala Val Lys Ser
100 105 110
Ser Glu His Ile Asn Glu Gly Glu Thr Ala Met Leu Val Cys Lys Ser
115 120 125
Glu Ser Val Pro Pro Val Thr Asp Trp Ala Trp Tyr Lys Ile Thr Asp
130 135 140
Ser Glu Asp Lys Ala Leu Met Asn Gly Ser Glu Ser Arg Phe Phe Val
145 150 155 160
Ser Ser Ser Gln Gly Arg Ser Glu Leu His Ile Glu Asn Leu Asn Met
165 170 175
Glu Ala Asp Pro Gly Gln Tyr Arg Cys Asn Gly Thr Ser Ser Lys Gly
180 185 190
Ser Asp Gln Ala Ile Ile Thr Leu Arg Val Arg Ser His Leu Ala Ala
195 200 205
Leu Trp Pro Leu Leu Gly Ile Val Ala Glu Val Leu Val Leu Val Thr
210 215 220
Ile Ile Phe Ile Tyr Glu Lys Arg Arg Lys Pro Glu Asp Val Leu Asp
225 230 235 240
Asp Asp Asp Ala Gly Ser Ala Pro Leu Lys Ser Ser Gly Gln His Gln
245 250 255
Asn Asp Lys Gly Lys Asn Val Arg Gln Arg Asn Ser Ser
260 265
<210> 33
<211> 1175
<212> PRT
<213> Artificial
<220>
<223> ACE2m mutation
<400> 33
Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala
1 5 10 15
Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe
20 25 30
Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp
35 40 45
Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn
50 55 60
Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala
65 70 75 80
Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln
85 90 95
Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys
100 105 110
Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser
115 120 125
Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu
130 135 140
Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu
145 150 155 160
Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu
165 170 175
Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg
180 185 190
Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu
195 200 205
Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu
210 215 220
Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu
225 230 235 240
His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile
245 250 255
Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly
260 265 270
Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys
275 280 285
Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala
290 295 300
Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu
305 310 315 320
Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro
325 330 335
Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly
340 345 350
Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp
355 360 365
Phe Leu Thr Ala His Asp Glu Met Gly Asp Ile Gln Tyr Asp Met Ala
370 375 380
Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe
385 390 395 400
His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys
405 410 415
His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn
420 425 430
Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly
435 440 445
Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe
450 455 460
Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met
465 470 475 480
Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr
485 490 495
Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe
500 505 510
Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala
515 520 525
Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile
530 535 540
Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu
545 550 555 560
Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala
565 570 575
Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe
580 585 590
Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr
595 600 605
Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu
610 615 620
Lys Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met
625 630 635 640
Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu
645 650 655
Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val
660 665 670
Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro
675 680 685
Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile
690 695 700
Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn
705 710 715 720
Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln
725 730 735
Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val
740 745 750
Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg
755 760 765
Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile
770 775 780
Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp
785 790 795 800
Val Gln Thr Ser Phe Ser Met Cys Val Pro Asp Gln Asp Thr Ala Ile
805 810 815
Arg Val Phe Ala Ile Pro Pro Ser Phe Ala Ser Ile Phe Leu Thr Lys
820 825 830
Ser Thr Lys Leu Thr Cys Leu Val Thr Asp Leu Thr Thr Tyr Asp Ser
835 840 845
Val Thr Ile Ser Trp Thr Arg Gln Asn Gly Glu Ala Val Lys Thr His
850 855 860
Thr Asn Ile Ser Glu Ser His Pro Asn Ala Thr Phe Ser Ala Val Gly
865 870 875 880
Glu Ala Ser Ile Cys Glu Asp Asp Trp Asn Ser Gly Glu Arg Phe Thr
885 890 895
Cys Thr Val Thr His Thr Asp Leu Pro Ser Pro Leu Lys Gln Thr Ile
900 905 910
Ser Arg Pro Lys Gly Val Ala Leu His Arg Pro Asp Val Tyr Leu Leu
915 920 925
Pro Pro Ala Arg Glu Gln Leu Asn Leu Arg Glu Ser Ala Thr Ile Thr
930 935 940
Cys Leu Val Thr Gly Phe Ser Pro Ala Asp Val Phe Val Gln Trp Met
945 950 955 960
Gln Arg Gly Gln Pro Leu Ser Pro Glu Lys Tyr Val Thr Ser Ala Pro
965 970 975
Met Pro Glu Pro Gln Ala Pro Gly Arg Tyr Phe Ala His Ser Ile Leu
980 985 990
Thr Val Ser Glu Glu Glu Trp Asn Thr Gly Glu Thr Tyr Thr Cys Val
995 1000 1005
Val Ala His Glu Ala Leu Pro Asn Arg Val Thr Glu Arg Thr Val
1010 1015 1020
Asp Lys Ser Thr Gly Lys Pro Thr Leu Tyr Asn Val Ser Leu Val
1025 1030 1035
Met Ser Asp Thr Ala Gly Thr Cys Tyr Lys Pro Lys Gly Met Thr
1040 1045 1050
Ser Ser Gln Trp Phe Lys Ile Gln His Met Gln Pro Ser Pro Gln
1055 1060 1065
Ala Cys Asn Ser Ala Met Lys Asn Ile Asn Lys His Thr Lys Arg
1070 1075 1080
Cys Lys Asp Leu Asn Thr Phe Leu His Glu Pro Phe Ser Ser Val
1085 1090 1095
Ala Ala Thr Cys Gln Thr Pro Lys Ile Ala Cys Lys Asn Gly Asp
1100 1105 1110
Lys Asn Cys His Gln Ser His Gly Ala Val Ser Leu Thr Met Cys
1115 1120 1125
Lys Leu Thr Ser Gly Lys His Pro Asn Cys Arg Tyr Lys Glu Lys
1130 1135 1140
Arg Gln Asn Lys Ser Tyr Val Val Ala Cys Lys Pro Pro Gln Lys
1145 1150 1155
Lys Asp Ser Gln Gln Phe His Leu Val Pro Val His Leu Asp Arg
1160 1165 1170
Val Leu
1175
<210> 34
<211> 627
<212> PRT
<213> Artificial
<220>
<223> fusion protein
<400> 34
Met Pro Leu Leu Leu Leu Leu Pro Leu Leu Trp Ala Gly Ala Leu Ala
1 5 10 15
Asp Ile Gln Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
20 25 30
Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser
35 40 45
Ser Ile Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
50 55 60
Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
65 70 75 80
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
85 90 95
Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
100 105 110
Tyr Tyr Ser Thr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
115 120 125
Lys Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly
130 135 140
Gly Ser Gly Gly Gly Ser Gly Met Gln Met Gln Leu Val Gln Ser Gly
145 150 155 160
Thr Glu Val Lys Lys Pro Gly Glu Ser Leu Lys Ile Ser Cys Lys Gly
165 170 175
Ser Gly Tyr Gly Phe Ile Thr Tyr Trp Ile Gly Trp Val Arg Gln Met
180 185 190
Pro Gly Lys Gly Leu Glu Trp Met Gly Ile Ile Tyr Pro Gly Asp Ser
195 200 205
Glu Thr Arg Tyr Ser Pro Ser Phe Gln Gly Gln Val Thr Ile Ser Ala
210 215 220
Asp Lys Ser Ile Asn Thr Ala Tyr Leu Gln Trp Ser Ser Leu Lys Ala
225 230 235 240
Ser Asp Thr Ala Ile Tyr Tyr Cys Ala Gly Gly Ser Gly Ile Ser Thr
245 250 255
Pro Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly
260 265 270
Ser Gly Gly Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu
275 280 285
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
290 295 300
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
305 310 315 320
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
325 330 335
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
340 345 350
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
355 360 365
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
370 375 380
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
385 390 395 400
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
405 410 415
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
420 425 430
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
435 440 445
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
450 455 460
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
465 470 475 480
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
485 490 495
Ser Leu Ser Pro Gly Lys Pro Asp Val Tyr Leu Leu Pro Pro Ala Arg
500 505 510
Glu Gln Leu Asn Leu Arg Glu Ser Ala Thr Ile Thr Cys Leu Val Thr
515 520 525
Gly Phe Ser Pro Ala Asp Val Phe Val Gln Trp Met Gln Arg Gly Gln
530 535 540
Pro Leu Ser Pro Glu Lys Tyr Val Thr Ser Ala Pro Met Pro Glu Pro
545 550 555 560
Gln Ala Pro Gly Arg Tyr Phe Ala His Ser Ile Leu Thr Val Ser Glu
565 570 575
Glu Glu Trp Asn Thr Gly Glu Thr Tyr Thr Cys Val Val Ala His Glu
580 585 590
Ala Leu Pro Asn Arg Val Thr Glu Arg Thr Val Asp Lys Ser Thr Gly
595 600 605
Lys Pro Thr Leu Tyr Asn Val Ser Leu Val Met Ser Asp Thr Ala Gly
610 615 620
Thr Cys Tyr
625
<210> 35
<211> 393
<212> PRT
<213> Artificial
<220>
<223> RNaseP
<400> 35
Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Ala Gly Ala Cys Ala Gly
1 5 10 15
Thr Cys Gly Cys Cys Gly Cys Thr Thr Cys Gly Thr Cys Gly Thr Cys
20 25 30
Cys Thr Cys Cys Thr Cys Thr Thr Cys Gly Gly Gly Gly Gly Ala Gly
35 40 45
Ala Cys Gly Gly Gly Cys Gly Gly Ala Gly Gly Gly Gly Ala Gly Gly
50 55 60
Ala Ala Ala Gly Thr Cys Cys Gly Gly Gly Cys Thr Cys Cys Ala Thr
65 70 75 80
Ala Gly Gly Gly Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Gly Gly
85 90 95
Thr Ala Ala Cys Gly Cys Cys Thr Gly Gly Gly Gly Gly Gly Gly Ala
100 105 110
Ala Ala Cys Cys Cys Ala Cys Gly Ala Cys Cys Ala Gly Thr Gly Cys
115 120 125
Ala Ala Cys Ala Gly Ala Gly Ala Gly Cys Ala Ala Ala Cys Cys Gly
130 135 140
Cys Cys Gly Ala Thr Gly Gly Cys Cys Cys Gly Cys Gly Cys Ala Ala
145 150 155 160
Gly Cys Gly Gly Gly Ala Thr Cys Ala Gly Gly Thr Ala Ala Gly Gly
165 170 175
Gly Thr Gly Ala Ala Ala Gly Gly Gly Thr Gly Cys Gly Gly Thr Ala
180 185 190
Ala Gly Ala Gly Cys Gly Cys Ala Cys Cys Gly Cys Cys Cys Gly Gly
195 200 205
Cys Thr Gly Gly Thr Ala Ala Cys Ala Gly Thr Cys Cys Gly Thr Gly
210 215 220
Gly Cys Ala Cys Gly Gly Thr Ala Ala Ala Cys Thr Cys Cys Ala Cys
225 230 235 240
Cys Cys Gly Gly Ala Gly Cys Ala Ala Gly Gly Cys Cys Ala Ala Ala
245 250 255
Thr Ala Gly Gly Gly Gly Thr Thr Cys Ala Thr Ala Ala Gly Gly Thr
260 265 270
Ala Cys Gly Gly Cys Cys Cys Gly Thr Ala Cys Thr Gly Ala Ala Cys
275 280 285
Cys Cys Gly Gly Gly Thr Ala Gly Gly Cys Thr Gly Cys Thr Thr Gly
290 295 300
Ala Gly Cys Cys Ala Gly Thr Gly Ala Gly Cys Gly Ala Thr Thr Gly
305 310 315 320
Cys Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Ala Ala Thr Gly
325 330 335
Ala Cys Thr Gly Thr Cys Cys Ala Cys Gly Ala Cys Ala Gly Ala Ala
340 345 350
Cys Cys Cys Gly Gly Cys Thr Thr Ala Thr Cys Gly Gly Thr Cys Ala
355 360 365
Gly Thr Thr Thr Cys Ala Cys Cys Thr Cys Ala Ala Ala Gly Ala Ala
370 375 380
Gly Ala Ala Gly Thr Gly Cys Cys Ala
385 390
<210> 36
<211> 394
<212> PRT
<213> Artificial
<220>
<223> RNaseP
<400> 36
Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Ala Gly Ala Cys Ala Gly
1 5 10 15
Thr Cys Gly Cys Cys Gly Cys Thr Thr Cys Gly Thr Cys Gly Thr Cys
20 25 30
Cys Thr Cys Cys Thr Cys Thr Thr Cys Gly Gly Gly Gly Gly Ala Gly
35 40 45
Ala Cys Gly Gly Gly Cys Gly Gly Ala Gly Gly Gly Gly Ala Gly Gly
50 55 60
Ala Ala Ala Gly Thr Cys Cys Gly Gly Gly Cys Thr Cys Cys Ala Thr
65 70 75 80
Ala Gly Gly Gly Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Gly Gly
85 90 95
Thr Ala Ala Cys Gly Cys Cys Thr Gly Gly Gly Gly Gly Gly Gly Ala
100 105 110
Ala Ala Cys Cys Cys Ala Cys Gly Ala Cys Cys Ala Gly Thr Gly Cys
115 120 125
Ala Ala Cys Ala Gly Ala Gly Ala Gly Cys Ala Ala Ala Cys Cys Gly
130 135 140
Cys Cys Gly Ala Thr Gly Gly Cys Cys Cys Gly Cys Gly Cys Ala Ala
145 150 155 160
Gly Cys Gly Gly Gly Ala Thr Cys Ala Gly Gly Thr Ala Ala Gly Gly
165 170 175
Gly Thr Gly Ala Ala Ala Gly Gly Gly Thr Gly Cys Gly Gly Thr Ala
180 185 190
Ala Gly Ala Gly Cys Gly Cys Ala Cys Cys Gly Cys Cys Cys Gly Gly
195 200 205
Cys Thr Gly Gly Thr Ala Ala Cys Ala Gly Thr Cys Cys Gly Thr Gly
210 215 220
Gly Cys Ala Cys Gly Gly Thr Ala Ala Ala Cys Thr Cys Cys Ala Cys
225 230 235 240
Cys Cys Gly Gly Ala Gly Cys Ala Ala Gly Gly Cys Cys Ala Ala Ala
245 250 255
Thr Ala Gly Gly Gly Gly Thr Thr Cys Ala Thr Ala Ala Gly Gly Thr
260 265 270
Ala Cys Gly Gly Cys Cys Cys Gly Thr Ala Cys Thr Gly Ala Ala Cys
275 280 285
Cys Cys Gly Gly Gly Thr Ala Gly Gly Cys Thr Gly Cys Thr Thr Gly
290 295 300
Ala Gly Cys Cys Ala Gly Thr Gly Ala Gly Cys Gly Ala Thr Thr Gly
305 310 315 320
Cys Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Ala Ala Thr Gly
325 330 335
Ala Cys Thr Gly Thr Cys Cys Ala Cys Gly Ala Cys Ala Gly Ala Ala
340 345 350
Cys Cys Cys Gly Gly Cys Thr Thr Ala Thr Cys Gly Gly Thr Cys Ala
355 360 365
Gly Thr Thr Thr Cys Ala Cys Cys Thr Cys Thr Gly Ala Thr Ala Thr
370 375 380
Cys Ala Cys Ala Cys Ala Thr Cys Cys Ala
385 390
<210> 37
<211> 394
<212> PRT
<213> Artificial
<220>
<223> RNaseP
<400> 37
Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Ala Gly Ala Cys Ala Gly
1 5 10 15
Thr Cys Gly Cys Cys Gly Cys Thr Thr Cys Gly Thr Cys Gly Thr Cys
20 25 30
Cys Thr Cys Cys Thr Cys Thr Thr Cys Gly Gly Gly Gly Gly Ala Gly
35 40 45
Ala Cys Gly Gly Gly Cys Gly Gly Ala Gly Gly Gly Gly Ala Gly Gly
50 55 60
Ala Ala Ala Gly Thr Cys Cys Gly Gly Gly Cys Thr Cys Cys Ala Thr
65 70 75 80
Ala Gly Gly Gly Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Gly Gly
85 90 95
Thr Ala Ala Cys Gly Cys Cys Thr Gly Gly Gly Gly Gly Gly Gly Ala
100 105 110
Ala Ala Cys Cys Cys Ala Cys Gly Ala Cys Cys Ala Gly Thr Gly Cys
115 120 125
Ala Ala Cys Ala Gly Ala Gly Ala Gly Cys Ala Ala Ala Cys Cys Gly
130 135 140
Cys Cys Gly Ala Thr Gly Gly Cys Cys Cys Gly Cys Gly Cys Ala Ala
145 150 155 160
Gly Cys Gly Gly Gly Ala Thr Cys Ala Gly Gly Thr Ala Ala Gly Gly
165 170 175
Gly Thr Gly Ala Ala Ala Gly Gly Gly Thr Gly Cys Gly Gly Thr Ala
180 185 190
Ala Gly Ala Gly Cys Gly Cys Ala Cys Cys Gly Cys Cys Cys Gly Gly
195 200 205
Cys Thr Gly Gly Thr Ala Ala Cys Ala Gly Thr Cys Cys Gly Thr Gly
210 215 220
Gly Cys Ala Cys Gly Gly Thr Ala Ala Ala Cys Thr Cys Cys Ala Cys
225 230 235 240
Cys Cys Gly Gly Ala Gly Cys Ala Ala Gly Gly Cys Cys Ala Ala Ala
245 250 255
Thr Ala Gly Gly Gly Gly Thr Thr Cys Ala Thr Ala Ala Gly Gly Thr
260 265 270
Ala Cys Gly Gly Cys Cys Cys Gly Thr Ala Cys Thr Gly Ala Ala Cys
275 280 285
Cys Cys Gly Gly Gly Thr Ala Gly Gly Cys Thr Gly Cys Thr Thr Gly
290 295 300
Ala Gly Cys Cys Ala Gly Thr Gly Ala Gly Cys Gly Ala Thr Thr Gly
305 310 315 320
Cys Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Ala Ala Thr Gly
325 330 335
Ala Cys Thr Gly Thr Cys Cys Ala Cys Gly Ala Cys Ala Gly Ala Ala
340 345 350
Cys Cys Cys Gly Gly Cys Thr Thr Ala Thr Cys Gly Gly Thr Cys Ala
355 360 365
Gly Thr Thr Thr Cys Ala Cys Cys Thr Thr Cys Thr Gly Ala Thr Cys
370 375 380
Cys Cys Ala Ala Thr Ala Thr Cys Cys Ala
385 390
<210> 38
<211> 394
<212> PRT
<213> Artificial
<220>
<223> RNaseP
<400> 38
Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Ala Gly Ala Cys Ala Gly
1 5 10 15
Thr Cys Gly Cys Cys Gly Cys Thr Thr Cys Gly Thr Cys Gly Thr Cys
20 25 30
Cys Thr Cys Cys Thr Cys Thr Thr Cys Gly Gly Gly Gly Gly Ala Gly
35 40 45
Ala Cys Gly Gly Gly Cys Gly Gly Ala Gly Gly Gly Gly Ala Gly Gly
50 55 60
Ala Ala Ala Gly Thr Cys Cys Gly Gly Gly Cys Thr Cys Cys Ala Thr
65 70 75 80
Ala Gly Gly Gly Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Gly Gly
85 90 95
Thr Ala Ala Cys Gly Cys Cys Thr Gly Gly Gly Gly Gly Gly Gly Ala
100 105 110
Ala Ala Cys Cys Cys Ala Cys Gly Ala Cys Cys Ala Gly Thr Gly Cys
115 120 125
Ala Ala Cys Ala Gly Ala Gly Ala Gly Cys Ala Ala Ala Cys Cys Gly
130 135 140
Cys Cys Gly Ala Thr Gly Gly Cys Cys Cys Gly Cys Gly Cys Ala Ala
145 150 155 160
Gly Cys Gly Gly Gly Ala Thr Cys Ala Gly Gly Thr Ala Ala Gly Gly
165 170 175
Gly Thr Gly Ala Ala Ala Gly Gly Gly Thr Gly Cys Gly Gly Thr Ala
180 185 190
Ala Gly Ala Gly Cys Gly Cys Ala Cys Cys Gly Cys Cys Cys Gly Gly
195 200 205
Cys Thr Gly Gly Thr Ala Ala Cys Ala Gly Thr Cys Cys Gly Thr Gly
210 215 220
Gly Cys Ala Cys Gly Gly Thr Ala Ala Ala Cys Thr Cys Cys Ala Cys
225 230 235 240
Cys Cys Gly Gly Ala Gly Cys Ala Ala Gly Gly Cys Cys Ala Ala Ala
245 250 255
Thr Ala Gly Gly Gly Gly Thr Thr Cys Ala Thr Ala Ala Gly Gly Thr
260 265 270
Ala Cys Gly Gly Cys Cys Cys Gly Thr Ala Cys Thr Gly Ala Ala Cys
275 280 285
Cys Cys Gly Gly Gly Thr Ala Gly Gly Cys Thr Gly Cys Thr Thr Gly
290 295 300
Ala Gly Cys Cys Ala Gly Thr Gly Ala Gly Cys Gly Ala Thr Thr Gly
305 310 315 320
Cys Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Ala Ala Thr Gly
325 330 335
Ala Cys Thr Gly Thr Cys Cys Ala Cys Gly Ala Cys Ala Gly Ala Ala
340 345 350
Cys Cys Cys Gly Gly Cys Thr Thr Ala Thr Cys Gly Gly Thr Cys Ala
355 360 365
Gly Thr Thr Thr Cys Ala Cys Cys Thr Gly Cys Gly Ala Gly Cys Thr
370 375 380
Cys Thr Ala Thr Thr Cys Thr Cys Cys Ala
385 390
<210> 39
<211> 394
<212> PRT
<213> Artificial
<220>
<223> RNaseP
<400> 39
Gly Ala Ala Gly Cys Thr Gly Ala Cys Cys Ala Gly Ala Cys Ala Gly
1 5 10 15
Thr Cys Gly Cys Cys Gly Cys Thr Thr Cys Gly Thr Cys Gly Thr Cys
20 25 30
Cys Thr Cys Cys Thr Cys Thr Thr Cys Gly Gly Gly Gly Gly Ala Gly
35 40 45
Ala Cys Gly Gly Gly Cys Gly Gly Ala Gly Gly Gly Gly Ala Gly Gly
50 55 60
Ala Ala Ala Gly Thr Cys Cys Gly Gly Gly Cys Thr Cys Cys Ala Thr
65 70 75 80
Ala Gly Gly Gly Cys Ala Gly Gly Gly Thr Gly Cys Cys Ala Gly Gly
85 90 95
Thr Ala Ala Cys Gly Cys Cys Thr Gly Gly Gly Gly Gly Gly Gly Ala
100 105 110
Ala Ala Cys Cys Cys Ala Cys Gly Ala Cys Cys Ala Gly Thr Gly Cys
115 120 125
Ala Ala Cys Ala Gly Ala Gly Ala Gly Cys Ala Ala Ala Cys Cys Gly
130 135 140
Cys Cys Gly Ala Thr Gly Gly Cys Cys Cys Gly Cys Gly Cys Ala Ala
145 150 155 160
Gly Cys Gly Gly Gly Ala Thr Cys Ala Gly Gly Thr Ala Ala Gly Gly
165 170 175
Gly Thr Gly Ala Ala Ala Gly Gly Gly Thr Gly Cys Gly Gly Thr Ala
180 185 190
Ala Gly Ala Gly Cys Gly Cys Ala Cys Cys Gly Cys Cys Cys Gly Gly
195 200 205
Cys Thr Gly Gly Thr Ala Ala Cys Ala Gly Thr Cys Cys Gly Thr Gly
210 215 220
Gly Cys Ala Cys Gly Gly Thr Ala Ala Ala Cys Thr Cys Cys Ala Cys
225 230 235 240
Cys Cys Gly Gly Ala Gly Cys Ala Ala Gly Gly Cys Cys Ala Ala Ala
245 250 255
Thr Ala Gly Gly Gly Gly Thr Thr Cys Ala Thr Ala Ala Gly Gly Thr
260 265 270
Ala Cys Gly Gly Cys Cys Cys Gly Thr Ala Cys Thr Gly Ala Ala Cys
275 280 285
Cys Cys Gly Gly Gly Thr Ala Gly Gly Cys Thr Gly Cys Thr Thr Gly
290 295 300
Ala Gly Cys Cys Ala Gly Thr Gly Ala Gly Cys Gly Ala Thr Thr Gly
305 310 315 320
Cys Thr Gly Gly Cys Thr Thr Ala Gly Ala Thr Gly Ala Ala Thr Gly
325 330 335
Ala Cys Thr Gly Thr Cys Cys Ala Cys Gly Ala Cys Ala Gly Ala Ala
340 345 350
Cys Cys Cys Gly Gly Cys Thr Thr Ala Thr Cys Gly Gly Thr Cys Ala
355 360 365
Gly Thr Thr Thr Cys Ala Cys Cys Thr Cys Ala Cys Ala Cys Thr Cys
370 375 380
Ala Thr Thr Ala Gly Cys Thr Cys Cys Ala
385 390
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