用于产生乙酸补身烷基酯化合物的方法

技术领域

本发明提供了新的方法，用于在体外或体内进行相应补身烷基(drimanyl)醇来源的乙酰化而进行乙酰基转移酶催化的乙酸补身烷基酯化合物的产生。本发明还涉及从不同的微生物和植物来源鉴定具有相应的乙酰基转移酶活性的酶。本发明还涉及提供衍生自所述新鉴定的酶的酶突变体。本发明的另一个形态涉及提供这种酶和突变体的相应编码序列、重组载体和重组宿主细胞，它们适合于产生这种乙酰基转移酶和突变体并用于进行乙酸补身烷基酯化合物的新产生方法。本发明的另一个形态涉及根据本发明获得的这种乙酸补身烷基酯作为中间体的用途，用于产生气味剂、调味剂或芳香剂或昆虫/害虫防治成分。

背景技术

萜烯存在于大多数生物(微生物、动物和植物)中。这些化合物由称为异戊二烯单元的五碳单元构成并且通过存在于它们结构中的这些单元的数目进行分类。因此，单萜、倍半萜和二萜是分别含有10、15和20个碳原子的萜烯。例如，在植物界中广泛地存在有倍半萜。许多倍半萜分子因为它们的风味和芳香特性以及它们的美容、医疗和抗菌效果而众所周知。已经鉴定出许多倍半萜烃和倍半萜类化合物。已经开发了化学合成途径，但是仍然很复杂并且不总是具有成本效益。

萜烯的生物合成产生涉及称为萜合酶的酶。在植物界中存在多种倍半萜合酶，它们均使用相同的底物(法呢基二磷酸，FPP)，但是具有不同的产物构造。已经克隆了编码倍半萜合酶的基因和cDNA，并且表征了相应的重组酶。

倍半萜例如具有补身烷(drimane)结构的化合物，例如补身烷基醇，特别是折叶苔醇(albicanol)或补身醇(drimenol)的许多主要来源是天然含有倍半萜的植物或微生物。然而，这些天然来源中倍半萜的含量可能较低。即使可以得到，这样的补身烷基醇在它们的进一步加工期间也难以处理，主要是因为它们在环境温度下是固体。提供更易于处理的衍生物将是一种改进的方法，并简化了补身烷基醇的进一步加工。

Akita,H.等人在Tetrahedron:Asymmetry 11(2000).1375-1388中描述了脂肪酶催化乙酸(+)折叶苔基酯的不对称合成。这种现有技术的方法需要至少八个化学合成步骤和两个被脂肪酶催化的连续反应，以获得对映体纯的乙酸折叶苔基酯。另外，乙酰化反应在33℃下在二异丙醚和乙酸异丙烯基酯的存在下发生，该条件与生物合成途径所需的生理条件不相容。而且，在生理条件下，就我们所知，脂肪酶不能催化所需的酯交换反应。尽管通过脂肪酶形成酯是可能的，但是该活性强烈取决于所存在的水的含量。因此，在诸如本发明的体内环境的水性环境中，脂肪酶催化酯键的水解而不是乙酰化(Jaeger K.等人；FEMSMicrobiology Reviews,1994,15:1pp29-63)。

仍然需要提供产生乙酸补身烷基酯化合物的新方法，特别是可以在水性环境中例如在基于宿主细胞的方法(其通过糖底物的代谢而提供补身烷基醇前体)中用于完全生物化学合成乙酸补身烷基酯的方法。

发明内容

可以通过提供一类新的酶来解决上述问题，这些酶显示出乙酰基转移酶的活性，并通过乙酰化作用并使用乙酰基-CoA作为乙酰基供体，从相应的补身烷醇前体(如折叶苔醇或补身醇)中产生乙酸补身烷基酯，如乙酸折叶苔基酯或乙酸补身烯基(drimenyl)酯。由于它们的物理化学性质，特别是由于它们在环境温度下为液体，因此补身烷基醇的乙酰化衍生物可以用作更合适的材料。

附图说明

图1a：(+)-折叶苔醇、(-)-补身醇和双环法呢醇的结构，以及比图1c的补身烷结构更具体的补身烷部分。

图1b：反应路线，其说明了通过无环倍半萜前体FPP和折叶苔醇细胞生物学产生乙酸折叶苔基酯。

图1c：更概括的“补身烷结构”，标出了C＝C-双键的潜在位置。

图2：(A/B)使用修饰的酿酒酵母菌株YST069产生的乙酸折叶苔基酯的GC-FID分析，该菌株将折叶苔醇合酶XP_007369631.1与乙酰基转移酶CrDAT(A)或乙酰基转移酶FgaAT(B)共表达。由(A)的乙酰基转移酶CrDAT产生的乙酸折叶苔基酯的MS谱图显示在(C)中，并且与来自乙酸折叶苔基酯标准品的MS谱图相同。

图3：由九种乙酰基转移酶(CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6和CfACT1-8)产生的乙酸折叶苔基酯的相对量，发现它们对于折叶苔醇有活性(如实施例2中所述)。

图4：由九种乙酰转移酶(CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6和CfACT1-8)产生的乙酸补身烯基酯的相对量，发现它们对于折叶苔醇有活性(如实施例2中所述)。

图5：表达以下每种乙酰基转移酶的酿酒酵母细胞所产生的乙酸折叶苔基酯的相对量：CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6，CfACT1-8，ERR364415-1_contig_8546和DfATC13。

图6：表达以下每种乙酰基转移酶的酿酒酵母细胞所产生的乙酸补身烯基酯的相对量：CrDAT，FgaAT，OAH94415.1，TcTAT，GAO81666.1，CfACT1-6，CfACT1-8，XP_001258079.1，ERR364415-1_contig_8546和DfATC13。

图7：通过乙酰基转移酶CrDAT产生的乙酸补身烯基酯的MS谱图显示在(A)中，其与(B)中显示的来自乙酸补身烯基酯标准品的MS谱图相同。

图8：通过乙酰基转移酶CrDAT产生的乙酸双环法呢基酯的MS谱图显示在(A)中，其与来自乙酸双环法呢基酯标准品(B)的MS谱图相同。

图9：表达以下每种乙酰基转移酶的酿酒酵母细胞所产生的乙酸双环法呢基酯的相对量：CrDAT，FgaAT，TcTAT，CrMAT，GAO81666.1，CfACT1-6，CfACT1-8，BAU61551.1，PsSalAT，XP_001217250.1，ERR364415-1_contig_8546，PYI04555.1和DfACT13。

具体实施方式

bp 碱基对

kb 千碱基

CoA 辅酶A

DNA 脱氧核糖核酸

cDNA 互补DNA

DTT 二硫苏糖醇

FPP 法呢基二磷酸

GC 气相色谱

MS 质谱仪/质谱法

MVA 甲羟戊酸

PCR 聚合酶链反应

RNA 核糖核酸

mRNA 信使核糖核酸

miRNA 微RNA

siRNA 小干扰RNA

rRNA 核糖体RNA

tRNA 转移RNA

除非另有说明，否则以下技术术语定义适用：

就本申请目的而言，“乙酰基转移酶”或“具有乙酰基转移酶活性的多肽”或“能够转移乙酰基的多肽”更概括而言是指一类酰基转移酶EC 2.3.1的酶，特别是乙酰基-CoA：醇O-乙酰基转移酶EC 2.3.1.84。它显示了用乙酰基-CoA作为乙酰基供体，乙酰化至少一种从折叶苔醇、补身醇和双环法呢醇中选出的补身烷基醇的能力。乙酸补身烷基酯可以以其任何立体异构体的形式或以其混合物的形式产生。如果相应的醇前体作为单个乙酰基受体存在，则乙酸折叶苔基酯，乙酸补身烯基酯或乙酸双环法呢基酯可能是唯一的产物，或者如果提供两种或更多种补身烷基醇的混合物并且乙酰基转移酶不是底物特异性的，则可能是两种或更多种乙酸补身烷基酯的混合物的一部分。在选择性增加的情况下，乙酰基转移酶可以主要形成一种单一的乙酸补身烷基酯。如本文所述的乙酰基转移酶可以对作为底物的不同的补身烷基醇显示相同或不同的偏好或特异性。例如，第一类型的乙酰基转移酶可主要为乙酰化折叶苔醇，第二类型的乙酰基转移酶可主要为乙酰化补身醇，而第三类型的乙酰基转移酶可主要为乙酰化双环法呢醇。在这种情况下，如果将这样的补身烷基醇的混合物用作底物，则会形成乙酸折叶苔醇酯、乙酸补身醇酯或乙酸双环法呢基酯。在底物特异性的情况下，乙酰基转移酶可以选择性地形成一种单一的乙酸补身烷基酯，即使将这种补身烷基醇的混合物用作底物。特别地，乙酰化是在保留了补身烷基醇底物的相应立体化学构型的情况下进行的。

“乙酰基供体”是指化学实体或分子，其充当待从所述供体酶促转移至受体分子的乙酰基的源，例如具有官能羟基的分子，其随后可与所述乙酰基反应，以形成相应的乙酸酯。一种特定的乙酰基供体是乙酰辅酶A(乙酰基-CoA)。

术语“补身烷倍半萜”或“补身烷”是指具有如图1a所示的补身烷样碳骨架结构的环状萜烯，或更具体而言是指图1c的更上位的结构，其中可选存在的C＝C-双键的潜在位置用虚线标记。

术语“补身烷基醇”是指“补身烷倍半萜”或“补身烷”的羟基化衍生物。其例子是任何立体异构形式的折叶苔醇、补身醇和双环法呢醇。

术语“乙酸补身烷基酯”是指这样的乙酸补身烷基醇的乙酰基酯衍生物，例如乙酸折叶苔基酯，乙酸补身烯基酯和乙酸双环法呢基酯。

就本申请目的而言，“折叶苔醇”特别地涉及(+)-折叶苔醇(CAS：54632-04-1)。

就本申请目的而言，“补身醇”特别地涉及(-)-补身醇(CAS：468-68-8)。

就本申请目的而言，“双环法呢醇”特别地涉及(+)-双环法呢醇或[(4aS,8aS)-2,5,5,8a-四甲基-3,4,4a,5,6,7,8,8a-八氢-1-萘基]甲醇(IUPAC名称)

“法呢基二磷酸”是指(2E,6E)-3,7,11-三甲基十二碳-2,6,10-三烯-1-焦磷酸酯(FPP)。

就本申请目的而言，“Ambrox”涉及IUPAC名称：(-)-(3aR,5aS,9aS,9bR)-3a,6,6,9a-四甲基十二氢萘并[2,1-b]呋喃(CAS：6790-58-5)。

术语“萜合酶”或“倍半萜合酶”或“补身烷倍半萜合酶”在本文可互换使用。

术语“双功能萜合酶”或“具有双功能萜合酶活性的多肽”涉及如2018年5月31日提交的PCT/EP2018/064344中进一步定义的多肽。

术语“折叶苔基二磷酸合酶”或“具有折叶苔基二磷酸合酶活性的多肽”或“折叶苔基二磷酸合酶蛋白”或“具有产生折叶苔基二磷酸的能力”涉及一种多肽，其能够从无环萜烯焦磷酸酯，特别是法呢基二磷酸(FPP)开始，催化任何立体异构体或其混合物形式的折叶苔基二磷酸的合成。折叶苔基二磷酸可以是唯一的产品，或者可以是倍半萜混合物的一部分。所述混合物可以包含折叶苔基单磷酸和/或折叶苔醇。此类多肽描述于例如2018年5月29日提交的PCT/CN2018/088902。

如在PCT/CN2018/088902中描述的，“折叶苔基二磷酸合酶活性”是在“标准条件”下测定的。

术语“折叶苔醇合酶”或“具有折叶苔醇合酶活性的多肽”或“折叶苔醇合酶蛋白”是指一种多肽，其能够从无环萜烯焦磷酸酯，特别是法呢基二磷酸(FPP)开始，催化任何立体异构体或其混合物形式的折叶苔醇的合成。折叶苔醇可以是唯一的产物，或者可以是两种或更多种倍半萜的混合物的一部分。

术语“补身醇合酶”或“具有补身醇合酶活性的多肽”或“补身醇合酶蛋白”是指一种多肽，其能够从无环萜烯焦磷酸酯，特别是法呢基二磷酸(FPP)开始，催化任何立体异构体或其混合物形式的补身醇的合成。补身醇可以是唯一的产物，或者可以是两种或更多种倍半萜的混合物的一部分。

“折叶苔醇合酶活性”和补身醇合酶活性如例如PCT/EP2018/064344、WO2015/169871或WO 2015/176959中所述测定。

如本发明中使用的“磷酸酶”的酶具有在消耗水的情况下将正磷酸酯转化为相应的醇和正磷酸根的能力。在此包括酸性磷酸酶(具有最佳酸性反应的EC.3.1.3.2)和碱性磷酸酶(具有最佳碱性反应的EC.3.1.3.1)。

术语“生物学功能”，“功能”，“生物学活性”或“活性”是指如本文所述的萜合酶的如下能力：a)催化形成折叶苔基二磷酸和/或折叶苔醇，或化合物的混合物，其包含折叶苔基二磷酸，和/或折叶苔基单磷酸和/或折叶苔醇和/或一种或多种其他萜烯，特别是折叶苔基二磷酸；或b)催化形成补身烷基醇或两种或更多种补身烷基醇与可选地一种或多种其他萜烯的混合物。

术语“生物学功能”，“功能”，“生物学活性”或“活性”是指如本文所述的乙酰基转移酶催化形成乙酸补身烷基酯或两种或更多种乙酸补身烷基酯与可选的一种或多种其他乙酰化的化合物的混合物的能力。

术语“萜烯的混合物”或“倍半萜的混合物”是指萜烯或倍半萜的混合物，其包含折叶苔醇、补身醇和双环法呢醇中的至少一种，并且还可以包含一种或多种另外的萜烯和/或一种或多种另外的倍半萜。

“甲羟戊酸途径”也称为“异戊二烯途径”或“HMG-CoA还原酶途径”，是真核生物、古菌和某些细菌中必不可少的代谢途径。甲羟戊酸途径始于乙酰辅酶A，并产生两个五碳结构单元，称为异戊烯基焦磷酸(IPP)和二甲基烯丙基焦磷酸(DMAPP)。关键酶是乙酰乙酰基-CoA硫解酶，HMG-CoA合酶，HMG-CoA还原酶，甲羟戊酸激酶，磷酸甲羟戊酸激酶，甲羟戊酸二磷酸脱羧酶和异戊烯基焦磷酸异构酶。将甲羟戊酸途径与酶活性结合以产生萜烯前体GPP、FPP或GGPP，特别是FPP合酶，允许重组细胞生产萜烯。

如本文所用，术语“宿主细胞”或“经转化的细胞”是指一种细胞(或生物体)，其被改变以携带(harbor)至少一个核酸分子，例如，编码所需蛋白质或核酸序列的重组基因，其在转录时产生执行本文所述的生物催化方法或其他重组方法所需的至少一种本发明的功能性多肽。特别地，这样的宿主细胞或经转化的细胞提供了乙酰基转移酶，其可用于从相应的补身烷基醇制备至少一种乙酸补身烷基酯。它们还可以提供其他酶，例如折叶苔基二磷酸合酶蛋白，其可用于产生折叶苔基二磷酸和/或折叶苔基单磷酸和/或折叶苔醇，或含有折叶苔基二磷酸和/或折叶苔基单磷酸和/或折叶苔醇的萜烯的相应混合物。它们还可以提供用于制备至少一种补身烷基醇的萜烯合酶。宿主细胞特别是细菌细胞、真菌细胞或植物细胞或植物。宿主细胞可以包含已整合到宿主细胞的核或细胞器基因组中的重组基因。或者，宿主可以在染色体外含有重组基因。

术语“生物(体)”是指任何非人的多细胞或单细胞生物，例如植物或微生物。特别地，微生物是细菌、酵母、藻类或真菌。

术语“植物”可互换使用以包括植物细胞，包括植物原生质体，植物组织，产生再生植物或植物部分的植物细胞组织培养物，或植物器官诸如根、茎、叶、花、花粉、胚珠、胚、果实等。任何植物均可以用来实施本文实施方案的方法。

当特定的生物体或细胞天然地产生FPP或当其不天然地产生FPP但是用本文所述的核酸转化以产生FPP时意味着“能够产生FPP”。经转化以比天然存在的生物体或细胞产生更高量的FPP的生物体或细胞也被“能够产生FPP的生物体或细胞”所涵盖。

当特定的生物体或细胞天然地产生乙酸补身烷基酯或当其不天然地产生乙酸补身烷基酯但用本文所述的核酸转化以产生乙酸补身烷基酯时意味着“能够产生乙酸补身烷基酯”。经转化以比天然存在的生物体或细胞产生更高量的乙酸补身烷基酯的生物体或细胞也被“能够产生乙酸补身烷基酯的生物体或细胞”所涵盖。

当特定的生物体或细胞天然地产生补身烷基醇或当其不天然产生补身烷基醇但经转化以产生补身烷基二磷酸，并视需要进一步用核酸转化以产生将补身烷基二磷酸转化为补身烷基醇的酶活性时意味着“能够产生补身烷基醇”。经转化以产生比天然存在的生物体或细胞更高量的补身烷基醇的生物体或细胞也被“能够产生补身烷基醇的生物体或细胞”所涵盖。

对于本文的说明书和所附权利要求，除非另有说明，否则“或”的使用意味着“和/或”。类似地，各种时态的“含”、“含有”、“包含”和“包括”是可互换的而不是限制性的。

应进一步理解，在各种实施方案的描述使用术语“包含”的情况下，本领域技术人员将理解，在一些特定情况下，可以使用“基本上由……组成”或“由……组成”的语言来替代地描述实施方案。

本文所用的术语“纯化的”、“基本上纯化的”和“分离的”是指不含其他不同化合物(本发明化合物通常以其天然状态与其缔合)的状态，因此“纯化的”、“基本上纯化的”和“分离的”物品占给定样品质量按重量计至少0.5％、1％、5％、10％或20％，或至少50％或75％。在一个实施方案中，这些术语是指本发明的化合物占给定样品质量按重量计至少95％、96％、97％、98％、99％或100％。如本文所用，当提及核酸或蛋白质时，核酸或蛋白质的术语“纯化的”、“基本上纯化的”和“分离的”也指一种纯化或浓缩状态，其不同于天然存在于例如原核或真核环境中，例如在细菌或真菌细胞中，或哺乳动物特别是人体中的状态。任何纯化程度或浓度，只要大于天然存在的纯化或浓缩程度，包括(1)从其他相关结构或化合物中的纯化，或(2)与在所述原核或真核环境中通常不相关的结构或化合物的缔合，都在“分离的”含义内。根据本领域技术人员已知的各种方法和工艺，本文所述的核酸、蛋白质或核酸或蛋白质的类别可以是分离的，或如若不然与它们通常在性质上不相关的结构或化合物相缔合。

术语“约”表示所述值的±25％的可能变化，特别是±15％、±10％、更特别是±5％、±2％或±1％。

术语“基本上”描述的值范围为约80至100％，例如85至99.9％，特别是90至99.9％，更特别是95至99.9％，或98至99.9％，尤其是99至99.9％。

“主要地”是指大于50％的范围内的比例，例如在51％至100％的范围内，特别是在75％至99.9％的范围内；尤其是85至98.5％，例如95至99％。

在本发明的上下文中，“主要产物”表示单一化合物或一组至少2种化合物，例如2、3、4、5或更多种，特别是2或3种化合物，该单一化合物或一组化合物“主要”是通过本文所述的反应制备的，并且基于由所述反应形成的产物的成分的总量，以主要比例包含在所述反应中。所述比例可以是摩尔比例，重量比例，或者优选基于色谱分析，由反应产物的相应色谱图计算的面积比例。

在本发明的上下文中，“副产物”表示单一化合物或一组至少2种化合物，例如2、3、4、5或更多种，特别是2或3种化合物，该单一化合物或一组化合物并非“主要”是通过本文所述的反应制备的。

由于酶促反应的可逆性，除非另有说明，否则本发明涉及在两个反应方向上本文所述的酶促或生物催化反应。

本文描述的多肽的“功能突变体”包括如下定义的此类多肽的“功能等同物”。

术语“立体异构体”特别包括构象异构体。

根据本发明，通常包括本文描述的化合物的所有“立体异构形式”，例如结构异构体，尤其是立体异构体及其混合物，例如旋光异构体或几何异构体，例如E和Z异构体，以及它们的组合。如果在一个分子中存在几个不对称中心，则本发明包括这些不对称中心的不同构象的所有组合，例如对映异构体对。

“立体选择性”描述了产生立体异构纯形式的化合物的特定立体异构体的能力或以本文所述的酶催化方法从多种立体异构体中特异性转化特定立体异构体的能力。更具体而言，这意味着本发明的产物相对于特定的立体异构体富集，或者离析物相对于特定的立体异构体可以贫化。这可以通过根据下式计算的纯度％ee参数进行量化：

％ee＝[X

其中X

术语“有选择地转化”或“增加选择性”通常是指，在所述反应的整个过程期间(即在反应的起始和终止之间)，在所述反应的某个时间点，或在所述反应的“一段”期间，特定的立体异构形式例如E-形式的不饱和烃以比相应的其他立体异构体形式例如Z-形式更高的比例或量(以摩尔为基准对比)被转化。特别地，在“一段”期间可以观察到所述选择性对应于底物初始量的1至99％，2至95％，3至90％，5至85％，10至80％，15至75％，20至70％，25至65％，30至60％，或40至50％的转化率。所述更高的比例或量可以例如以以下方式表示：

-在整个反应过程或其所述一段期间观察到的较高的异构体最大收率；

-在确定的底物转化率值百分比下，较高的异构体相对含量；和/或

-在较高的转化率值百分比下，相同的异构体相对含量；

其中的每一种优选相对于参考方法来观察，所述参考方法在其他相同条件下用已知化学或生物化学方法进行。

根据本发明，通常包括本文描述的化合物的所有“异构体形式”，例如结构异构体，尤其是立体异构体及其混合物，例如旋光异构体或几何异构体，例如E和Z异构体，以及它们的组合。如果在一个分子中存在几个不对称中心，则本发明包括这些不对称中心的不同构象的所有组合，例如对映异构体对，或立体异构体形式的任何混合形式。

根据本发明的反应的“收率”和/或“转化率”是在例如4、6、8、10、12、16、20、24、36或48小时的规定时间段内(反应在该段时间内进行)确定的。特别地，反应在精确定义的条件下进行，例如在本文定义的“标准条件”下进行。

不同的收率参数(“收率”或YP/S；“比生产率收率”；或时空收率(STY))在本领域中是众所周知的，并且如文献所述进行测定。

“收率”和“YP/S”(均以所生产的产品质量/所消耗的材料质量表示)在本文中用作同义词。

比生产率收率(specific productivity-yield)描述了每小时每L发酵液每克生物质所生产的产物的量。用WCW表示的湿细胞重量描述了生化反应中具有生物活性的微生物的数量。该值以每g WCW每小时的产品g数给出(即g/gWCW

术语“发酵产生”或“发酵”是指微生物(由所述微生物所包含或由其产生的酶活性辅助)在细胞培养物中利用添加到温育中的至少一种碳源产生化合物的能力。

术语“发酵液”应理解为是指一种液体，特别是水性溶液或水性/有机溶液，其基于发酵工艺并且未进行或进行了例如本文所述的后处理(work up)。

“酶催化”或“生物催化”方法是指所述方法在酶(包括本文所定义的酶突变体)的催化作用下进行。因此，该方法可以在分离形式的(纯化的、富集的)或粗制形式的所述酶的存在下，或者在细胞系统的存在下进行，所述细胞系统特别是包含活性形式的所述酶并具有如本文所公开的催化转化反应能力的天然或重组微生物细胞。

如果本公开涉及不同优先程度的特征、参数及其范围(包括上位的，非明确优选的特征、参数及其范围)，则除非另有说明，否则这些特征、参数和范围中的两个或更多个的任意组合与它们各自的优选程度无关地涵盖在本发明的公开内容中。

a.本发明的特定实施方案

1.产生至少一种，特别是一种、两种或三种，特别是一种或两种乙酸补身烷基酯化合物的生物催化方法，

包括以下步骤：

(1)在乙酰基供体的存在下，使至少一种，特别是一种立体异构纯形式或立体异构体混合物形式的补身烷基醇与至少一种，特别是一种多肽接触，该多肽具有特别是能将乙酰基从所述乙酰基供体转移至所述至少一种，特别是一种补身烷基醇的乙酰基转移酶活性，从而获得至少一种作为主要产物的乙酸补身烷基酯，特别是一种作为主要产物的乙酸补身烷基酯；和

(2)可选地，从步骤(1)的反应产物中分离出所述至少一种，特别是一种乙酸补身烷基酯化合物。

如果形成多于一种的乙酸补身烷基酯，则可以进一步分离混合物并且可以纯化各个乙酸酯。

2.根据实施方案1所述的方法，其中，所述乙酸补身烷基酯化合物从由如下构成的群组中选出：乙酸折叶苔基酯，乙酸补身烯基酯，和乙酸双环法呢基酯，其各自为立体异构纯形式或作为其至少两种立体异构体的混合物，或它们的组合，其包括所述群组的至少两个成员。在一个特定的实施方案中，仅一种补身烷基醇用作底物，并且仅一种乙酸补身烷基酯以立体异构纯形式或作为其至少两种立体异构体的混合物，特别是立体异构纯形式的产物获得。

3.根据实施方案1或2所述的方法，其中所述补身烷基醇从由如下构成的群组中选出：折叶苔醇，特别是(+)-折叶苔醇，补身醇，特别是(-)-补身醇，和双环法呢醇，特别是(+)-双环法呢醇，其各自为立体异构纯形式或作为其至少两种立体异构体的混合物，或它们的组合，其包括所述群组的至少两个成员。在一个特定的实施方案中，仅一种特别是立体异构纯形式的补身烷基醇用作底物。

4.根据前述实施方案中任一项所述的方法，其中所述乙酰基供体是乙酰辅酶A(乙酰基-CoA)。所述供体可以被外源地添加到反应混合物中，例如在使用分离的、富集的或纯化的酶的体外过程中，或更具体而言是内源地存在的，例如在使用宿主细胞系统的体内过程中，该系统产生乙酰辅酶A系统作为代谢物并表达所需的一种或多种多肽，以进行预期的乙酰化或更复杂的多步骤过程，该过程包括所述乙酰化作为一个步骤。

5.根据前述实施方案中任一项所述的方法，其中所述乙酰基转移酶选自：

a)包含选自SEQ ID NO:9、11、13、15、17、19、21、23、25、118、121、124、127、130、133、136、143和144的氨基酸序列的多肽，和

b)具有乙酰基转移酶活性并包含与SEQ ID NO:9、11、13、15、17、19、21、23、25、118、121、124、127、130、133、136、143和144的至少一个所述氨基酸序列显示至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％，或99％序列同一性程度的氨基酸序列的多肽。

下面的列表说明了本发明的特定乙酰基转移酶产生一种或多种从由乙酸折叶苔基酯，乙酸补身烯基酯和乙酸双环法呢基酯构成的群组中选出的乙酸补身烷基酯化合物的能力：

6.根据前述实施方案中任一项所述的方法，还包括在步骤1)之前，所述至少一种，特别是一种补身烷基醇化合物的生物催化形成。

7.根据实施方案6所述的方法，其中所述补身烷基醇化合物可以内源地存在于反应混合物中，例如在使用宿主细胞系统的体内过程中，该系统产生所述补身烷基醇化合物作为代谢产物并表达所需的一种或多种多肽用于进行预期的补身烷基醇的合成或更复杂的多步骤过程，该过程包括所述补身烷基醇的合成作为一个步骤，由此从非环状倍半萜前体酶促合成所述补身烷基醇。

或者，所述补身烷基醇化合物通过化学或酶促方法产生，并被外源地添加到反应混合物中，例如在使用其形成所需的如下定义的分离的、富集的或纯化的合酶的体外过程中。

8.根据实施方案7所述的方法，其中所述非环状倍半萜前体是法呢基焦磷酸(FPP)。

9.根据实施方案7至8中任一项所述的方法，其中该补身烷基醇的所述酶促合成是通过一种或多种多肽来催化的，该多肽具有在一个或多个酶促步骤中将所述非环状倍半萜前体转化为至少一种补身烷基醇，特别是一种补身烷基醇，特别是作为主要产物的能力。

10.根据实施方案6至9中任一项所述的方法，其中所述至少一种补身烷基醇是在单个或多个，特别是两个酶促步骤中由FPP产生的。

11.根据实施方案10所述的方法，其中所述至少一种补身烷基醇是通过FPP的酶促转化，由如下物质催化产生的：

a)具有形成所述补身烷基醇的补身烷倍半萜合酶活性的多肽(单步生物合成)；或者

b)形成具有至少一种磷酸补身烷基酯中间体的磷酸补身烷基酯合酶活性的多肽与具有将所述至少一种磷酸补身烷基酯(单磷酸酯和/或二磷酸酯)中间体转化为至少一种补身烷基醇的磷酸酶活性的多肽的组合(两步生物合成)。

12.根据实施方案11所述的方法，其中，

a)所述具有补身烷倍半萜合酶活性的多肽选自具有折叶苔醇合酶活性，补身醇合酶活性，双环法呢醇合酶活性或这些活性的任何组合的多肽，该多肽特别是优先显示所述活性之一，更特别地，特异性地显示所述活性之一；和

b)多肽的所述组合包括补身烷基二磷酸合酶活性，特别是折叶苔基二磷酸合酶活性和磷酸酶，例如细菌碱性磷酸酶。

13.根据实施方案12所述的方法，其中，

a)所述具有补身烷倍半萜合酶活性的多肽选自如PCT/EP2018/064344(2018年5月31日提交)中所述的补身烷合酶和如公开的WO2015/169871和WO 2015/176959中所述的补身醇合酶；

b)所述具有磷酸补身烷基酯合酶活性的多肽是2018年5月29日提交的PCT/CN2018/088902中所述的折叶苔基二磷酸合酶，包括从作为底物的法呢基二磷酸(FPP)产生折叶苔基磷酸酯衍生物如单磷酸酯，更特别是折叶苔基二磷酸的能力。

如PCT/CN2018/088902(2018年5月29日提交)中所述的折叶苔基二磷酸合酶为：

香鳞毛蕨(Dryopteris fragrans)的DfHAD，DfHAD-9(V274A)，DfHAD-His_GST和DfHAD-8(K532R)

以及由其衍生的与其具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的多肽。

如PCT/EP2018/064344(2018年5月31日提交)中所述的补身烷合酶(即折叶苔醇合酶或补身醇合酶)是

以及由此衍生的与其具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的多肽。

如WO2015/169871中所述的补身醇合酶是

以及由此衍生的与其具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的多肽。

如WO 2015/176959中所述的补身醇合酶是

黑水缬草(Valeriana amurensis)的VaTPS3

以及由此衍生的与其具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的多肽。

在本说明书的末尾列出了上述合酶的各个氨基酸和核苷酸序列的SEQ ID NO。这些多肽和核酸以及由其衍生的与这些序列中的至少一个具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的多肽和核酸也是本公开的一部分。

14.根据实施方式13所述的方法，其中，所述补身烷倍半萜合酶选自：

a)具有(双功能)折叶苔醇合酶活性并且包含氨基酸序列SEQ ID NO:5的多肽，或具有折叶苔醇合酶活性并且包含与在PCT/EP2018/064344中描述的SEQ ID NO:5具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的氨基酸序列的突变体或变体多肽；

b)具有(双功能)补身醇合酶活性并且包含氨基酸序列SEQ ID NO:7的多肽，或具有补身醇合酶活性并且包含与在PCT/EP2018/064344中描述的SEQ ID NO:7具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％序列同一性的氨基酸序列的突变体或变体多肽。

15.根据前述实施方案中任一项所属的方法，其在宿主细胞培养物中在体内或在包含产生至少一种乙酸补身烷基酯所需的宿主细胞裂解物或者富集或分离的多肽的液体反应介质中在体外进行，每种均在有利于产生至少一种乙酸补身烷基酯的条件下进行。

特别地，该反应在内源形成的或外源添加的乙酰基-CoA作为乙酰基供体的存在下进行。特别地，该反应在内源形成的或外源添加的FPP的存在下进行。内源形成的FPP特别是生物转化为FPP的至少一种碳源例如糖底物的代谢的结果。细胞体内方法尤其令人感兴趣，其中FPP和乙酰基-CoA是内源形成的。

这些宿主细胞或生物体中的某些不天然地产生FPP。为了适合于实施本文所述的实施方案的方法，不天然地产生无环萜烯焦磷酸前体例如FPP的生物体或细胞被基因修饰以产生所述前体。例如，它们可以在用根据以上任何一个实施方案所述的核酸修饰之前或同时如此转化。转化生物体以使其产生无环萜烯焦磷酸前体例如FPP的方法是本领域已知的。例如，引入甲羟戊酸途径的酶活性是使生物体产生FPP的合适策略。

16.根据实施方案15所述的方法，其在能够功能性表达以下项的重组宿主细胞或重组非人宿主生物体中进行：

a)至少一种实施方案5中定义的乙酰基转移酶；可选地

b)至少一种多肽，其具有将非环状倍半萜前体FPP转化为实施方案9至14中任一项定义的至少一种补身烷基醇的能力；以及可选地

c)至少一种从如上定义的甲羟戊酸途径的酶中选择的酶。

在一个特定的实施方案中，酶a)和b)或者酶a)、b)和c)通过本发明的体内方法中应用的细胞系统功能性表达。

17.根据实施方案16所述的方法，其中所述非人宿主细胞或宿主生物体选自原核或真核微生物，或由其衍生的细胞。

18.根据实施方案17所述的方法，其中所述非人宿主细胞或宿主生物体选自细菌、真菌和植物细胞或植物。

19.根据实施方案18所述的方法，其中所述真菌细胞是酵母细胞，特别是选自酵母属(Saccharomyces)、毕赤酵母属(Pichia)或耶氏酵母属(Yarrowia)，特别是选自酿酒酵母(Saccharomyces cerevisiae)、巴斯德毕赤酵母(Pichia pastoris)或解脂耶氏酵母(Yarrowia lipolytica)种。

20.根据实施方案18所述的方法，其中所述细菌细胞选自红球菌属(Rhodococcus)、假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)或埃希氏菌属(Escherichia)，特别是大肠杆菌(E.coli)种。

21.根据前述实施方案中任一项所述的方法，其进一步包括作为步骤(3)，使用化学或生物催化合成或两者的组合来处理步骤(1)或步骤(2)的至少一种乙酸补身烷基酯以获得衍生物。

22.根据实施方案21所述的方法，其中所述衍生物是烃，醇，二醇，三醇，缩醛，缩酮，醛，酸，醚，酰胺，酮，内酯，环氧化物，乙酸酯，糖苷，酯和/或多环化合物。

23.根据前述实施方案中任一项所述的方法，其中所述乙酸补身烷基酯包括乙酸折叶苔基酯，乙酸补身烯基酯或乙酸双环法呢基酯，特别是乙酸折叶苔基酯或乙酸补身烯基酯，作为主要的补身烷基醇产物或特别是作为单一的补身烷基醇产物。

24.根据前述实施方案中任一项所述的方法，其包括特别是通过转化向非人宿主生物体或宿主细胞提供以下物质：

a)至少一种核酸、表达构建体或载体，其包含编码至少一种具有乙酰基转移酶活性的多肽的核酸序列，该多肽能够将乙酰基从乙酰基供体转移至补身烷基醇，可选地稳定地整合到基因组中；可选地

b)至少一种核酸、表达构建体或载体，其包含编码至少一种具有补身烷基醇合酶活性的多肽的核酸序列，该多肽能够从非环状倍半萜前体产生补身烷基醇，可选地稳定地整合到基因组中；以及可选地

c)至少一种核酸、表达构建体或载体，其包含编码至少一种涉及生物合成途径的多肽的核酸序列，该多肽用于产生所述非环状倍半萜前体，可选地稳定地整合到基因组中。

在一个特定的实施方案中，非人宿主生物体或宿主细胞用a)和b)或者a)、b)和c)转化，并且更特别地包含稳定地整合到基因组中的所述核酸。该核酸a)、b)和/或c)可以位于相同或两个或更多个不同载体上。

25.一种具有乙酰基转移酶活性的多肽，其能够将乙酰基从乙酰基供体转移至补身烷基醇以产生乙酸补身烷基酯，其包含与从SEQ ID NO:9、11、13、15、17、19、21、23、25、118、121、124、127、130、133、136、143和144中选出的至少一个氨基酸序列具有40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％至少于100％序列同一性的氨基酸序列。

26.一种分离的核酸分子，

a)包含编码实施方案5的多肽的核苷酸序列；或者

b)包含与从SEQ ID NO:8、10、12、14、16、16、18、20、22、24、116、117、119、120、122、123、125、126、128、129、131、132、134和135中选出的核苷酸序列具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％，85％，90％，95％，96％，97％，98％或99％并少于100％序列同一性的核苷酸序列；或者

c)包含核苷酸序列，该核苷酸序列包含与序列a)或b)之一互补的序列；或者

d)包含在严格条件下与核苷酸序列a)、b)或c)杂交的核苷酸序列。

27.一种表达构建体，其包含至少一种实施方案26的核酸分子。

28.一种载体，其包含至少一种实施方案25的核酸分子或至少一种实施方案26的表达构建体。

29.根据实施方案28所述的载体，其中该载体是原核、病毒或真核载体。

30.根据实施方案28或29所述的载体，其中该载体是表达载体。

31.根据实施方案28至30中任一项所述的载体，其是质粒载体。

32.一种重组宿主细胞或重组非人宿主生物体，其包含：

a)至少一种实施方案26的分离的核酸分子，其可选地稳定地整合到基因组中；或者

b)至少一种实施方案27的表达构建体，其可选地稳定地整合到基因组中；或者

c)至少一种实施方案28至31中任一项的载体。

在一个特定的实施方案中，非人宿主生物体或宿主细胞用a)和b)或者a)、b)和c)转化，并且更特别地包含稳定地整合到基因组中的所述核酸。

33.根据实施方案32所述的宿主细胞或宿主生物体，其选自原核或真核微生物，或由其衍生的细胞。

34.根据实施方案33所述的宿主细胞或宿主生物体，其选自细菌、真菌和植物细胞或植物。

35.根据实施方案34所述的宿主细胞或宿主生物体，其中所述真菌细胞是酵母细胞。

36.根据实施方案35所述的宿主细胞或宿主生物体，其中所述细菌细胞选自埃希氏菌属(Escherichia)，特别是大肠杆菌(E.coli)种，并且所述酵母细胞选自酵母属(Saccharomyces)、毕赤酵母属(Pichia)或耶氏酵母属(Yarrowia)，特别是选自酿酒酵母(Saccharomyces cerevisiae)、巴斯德毕赤酵母(Pichia pastoris)或解脂耶氏酵母(Yarrowia lipolytica)种。

37.一种用于产生至少一种根据实施方案25的催化活性多肽的方法，包括：

a)培养实施方案32至34之一的非人宿主生物体或宿主细胞以表达或过表达根据实施方案25的至少一种多肽；和

b)可选地，从步骤a)中培养的非人宿主细胞或生物体中分离多肽。

38.根据实施方案37所述的方法，其还包括在步骤a)之前，向非人宿主生物体或细胞提供以下物质，特别是用以下物质转化非人宿主生物体或宿主细胞：根据权利要求26的至少一种核酸，或权利要求27的至少一种构建体，或权利要求28至31中任一项的至少一种载体，从而其表达或过表达根据权利要求25的多肽。

39.一种制备具有乙酰基转移酶活性的突变多肽的方法，该突变多肽能够将乙酰基从乙酰基供体转移至至少一种，特别是一种补身烷基醇，以产生至少一种，特别是一种乙酸补身烷基酯，该方法包括以下步骤：

a)选择出编码从SEQ ID NO:9、11、13、15、17、19、21、23、25、118、121、124、127、130、133、136、143和144中选出的多肽的核酸分子，

b)修饰所选择的核酸分子以获得至少一种突变核酸分子；

c)用突变核酸序列转化宿主细胞或单细胞宿主生物体以表达由该突变核酸序列编码的多肽；

d)筛选表达产物中至少一种具有乙酰基转移酶活性的突变体；和，

e)可选地，如果该多肽不具有所需突变活性，则重复步骤a)至d)，直到获得具有所需突变活性的多肽；和

f)可选地，如果在步骤d)中鉴定出具有所需突变活性的多肽，则分离在步骤c)中获得的相应突变核酸。

40.前述实施方案中任一项定义的乙酰基转移酶的用途，用于制备气味剂、调味剂或芳香剂成分或昆虫/害虫防治，例如用于制备从身体护理、家庭护理或芳香剂组合物中选出的组合物。

b.根据本发明适用的多肽

在本文语境中，以下定义适用：

可以互换使用的通用术语“多肽”或“肽”是指天然的或合成的，连续的、肽方式连接的氨基酸残基的线性链或序列，其包含约10个至多于1000个残基。具有最多30个残基的短链多肽也被称为“寡肽”。

术语“蛋白(质)”是指由一种或多种多肽组成的大分子结构。其多肽的氨基酸序列代表蛋白质的“一级结构”。氨基酸序列还通过形成特殊的结构元素(例如在多肽链中形成的α-螺旋和β-折叠结构)来预先确定蛋白质的“二级结构”。多个这样的二级结构元件的排列定义了蛋白质的“三级结构”或空间排列。如果蛋白质包含多于一个的多肽链，则所述链在空间上排列形成蛋白质的“四级结构”。蛋白质正确的空间排列或“折叠”是蛋白质功能的前提。变性或展开会破坏蛋白质功能。如果这种破坏是可逆的，则可以通过重新折叠来恢复蛋白质功能。

本文所指的典型的蛋白质功能是“酶功能”，即蛋白质在底物例如化合物上充当生物催化剂，并催化所述底物向产物的转化。酶可以显示高或低程度的底物和/或产物特异性。

因此，本文中被称为具有特定“活性”的“多肽”隐含地是指正确折叠的蛋白质，其显示出所指示的活性，例如特定的酶活性。

因此，除非另有说明，否则术语“多肽”也涵盖术语“蛋白质”和“酶”。

类似地，术语“多肽片段”涵盖术语“蛋白质片段”和“酶片段”。

术语“分离的多肽”是指通过本领域已知的任何方法或这些方法(包括重组、生物化学和合成法)的组合从其天然环境中取出的氨基酸序列。

“靶肽”是指一种氨基酸序列，其将蛋白质或多肽靶向细胞内细胞器(即，线粒体或质体)或细胞外空间(分泌信号肽)。编码靶肽的核酸序列可以被融合到编码蛋白或多肽的氨基末端(例如N-末端)的核酸序列，或者可以被用来替换天然靶向多肽。

本发明还涉及本文具体描述的多肽的“功能等同物”(也称为“类似物”或“功能突变”)。

例如，“功能等同物”是指一种多肽，其在用于确定酶活性的测试中，显示与本文具体描述的并作为所述比较基础的多肽相比，至少高或低1至10％、或至少20％、或至少50％、或至少75％、或至少90％的酶活性。

根据本发明，“功能等同物”还涵盖特定的突变体，其在本文所述的氨基酸序列的至少一个序列位置中具有与具体陈述的氨基酸不同的氨基酸，但是仍然具有上述生物活性之一，例如酶活性。因此，“功能等同物”包括可通过一个或多个，例如1至20个、1至15个或5至10个氨基酸的添加、取代特别是保守取代(即，作为其结果，所讨论的氨基酸被具有相同电荷、大小、极性和/或溶解度的氨基酸取代)、缺失和/或倒置而获得的突变体，其中所述变化可以在任何序列位置上发生，只要它们导致突变体具有本发明特性的概貌。还特别地提供功能等同性，如果活性模式与在突变体和未改变的多肽之间定性地重合，即，如果例如观察到与相同的激动剂或拮抗剂或底物的相互作用，但是速率不同(即，通过EC

上述意义上的“功能等同物”也是本文所述多肽的“前体”，以及所述多肽的“功能衍生物”和“盐”。

在该情况下，“前体”是具有或不具有所期望生物活性的多肽的天然或合成前体。

表述“盐”是指根据本发明的蛋白质分子的羧基的盐以及氨基的酸加成的盐。羧基的盐可以已知的方式生产，包括无机盐，例如钠、钙、铵、铁和锌盐，以及与有机碱例如胺，如三乙醇胺、精氨酸、赖氨酸、哌啶等形成的盐。酸加成的盐，例如与无机酸例如盐酸或硫酸形成的盐，以及与有机酸例如乙酸和草酸形成的盐，也被本发明所涵盖。

根据本发明的多肽的“功能衍生物”还可以使用已知技术在功能性氨基酸侧基或它们的N末端或C末端产生。这样的衍生物包括例如：羧酸基的脂族酯，羧酸基的酰胺，它们可通过与氨或与伯或仲胺反应获得；游离氨基的N-酰基衍生物，其通过与酰基反应生成；或游离羟基的O-酰基衍生物，其通过与酰基反应生成。

“功能等同物”自然也包括可以从其他生物体获得的多肽以及天然存在的变体。例如，可以通过序列比较来确定同源序列区域的面积，并且等同的多肽可以基于本发明的具体参数来确定。

“功能等同物”还包含根据本发明的多肽的“片段”，例如单个结构域或序列基序，或N末端和/或C末端截短的形式，其可以显示或可以不显示期望的生物学功能。优选地，这样的“片段”至少定性地保持期望的生物学功能。

此外，“功能等同物”是融合蛋白，其具有本文所述的多肽序列之一或由其衍生的功能等同物，以及在功能性N-末端或C-末端缔合(即，没有融合蛋白部分的实质性相互功能受损)中具有至少一个另外的功能不同的异源序列。这些异源序列的非限制性例子是例如信号肽、组氨酸锚或酶。

根据本发明还包括的“功能等同物”是与具体公开的多肽的同源物。它们与具体公开的氨基酸序列具有至少60％，优选至少75％，特别是至少80或85％，例如90、91、92、93、94、95、96、97、98或99％的同源性(或同一性)，其通过Pearson and Lipman,Proc.Natl.Acad,Sci.(USA)85(8),1988,2444-2448的算法计算。根据本发明的同源多肽的以百分比表示的同源性或同一性尤其是指基于本文具体描述的氨基酸序列之一的总长度，以氨基酸残基的百分比表示的同一性。

以百分比表示的同一性数据也可以借助于BLAST比对，算法blastp(蛋白质-蛋白质BLAST)或通过应用本文下面详述的Clustal设置来确定。

在可能的蛋白质糖基化的情况下，根据本发明的“功能等同物”包括本文所述的去糖基化或糖基化形式的多肽，以及可以通过改变糖基化模式获得的经修饰形式。

根据本发明的多肽的功能等同物或同源物可以通过诱变产生，例如通过点突变，延长或缩短蛋白质或如下文更详细描述。

根据本发明的多肽的功能等同物或同源物可以通过筛选突变体例如缩短的突变体的组合数据库来鉴定。例如，蛋白质变体的多样性数据库可以通过在核酸水平上的组合诱变，例如通过合成寡核苷酸混合物的酶促连接来产生。有许多方法可用于从简并寡核苷酸序列产生潜在同源物的数据库。简并基因序列的化学合成可以在自动DNA合成仪中进行，然后可以将合成基因连接在合适的表达载体中。简并基因组的使用使得可以提供混合物中的所有序列，其编码所需的潜在蛋白质序列集合。简并寡核苷酸的合成方法是本领域技术人员已知的。

在现有技术中，已知几种技术用于筛选通过点突变或缩短产生的组合数据库的基因产物，以及用于筛选具有选定性质的基因产物的cDNA文库。这些技术可以适用于快速筛选通过根据本发明的同源物的组合诱变产生的基因库。最常用于筛选大型基因库的基于高通量分析的技术包括在可复制的表达载体中克隆基因库，用所得载体数据库转化合适的细胞，以及在特定条件下表达组合基因，在所述条件下，所需活性的检测促进编码基因(其产物被检测)的载体的分离。递归整合诱变(REM)是一种提高数据库中功能突变体频率的技术，其可以与筛选测试结合使用，以鉴定同源物。

本文提供的实施方案提供了本文公开的多肽的直系同源物和旁系同源物，以及用于鉴定和分离此类直系同源物和旁系同源物的方法。术语“直系同源物”和“旁系同源物”的定义在下面给出，并适用于氨基酸和核酸序列。

c.根据本发明适用的编码核酸序列

在本文语境中，以下定义适用：

术语“核酸序列”、“核酸”、“核酸分子”和“多核苷酸”可互换使用，是指核苷酸的序列。核酸序列可以是任意长度的单链或双链脱氧核糖核苷酸或核糖核苷酸，并且包括基因的编码和非编码序列、外显子、内含子、有义和反义互补序列、基因组DNA、cDNA、miRNA、siRNA、mRNA、rRNA、tRNA、重组核酸序列、分离的和纯化的天然产生的DNA和/或RNA序列、合成的DNA和RNA序列、片段、引物和核酸探针。技术人员了解RNA的核酸序列与DNA序列相同，差异在于胸腺嘧啶(T)被替代为尿嘧啶(U)。术语“核苷酸序列”也应理解为包含单独的片段形式或作为较大核酸组分的多核苷酸分子或寡核苷酸分子。

“分离的核酸”或“分离的核酸序列”是指一种核酸或核酸序列，其所处的环境与天然产生的核酸或核酸序列所处的环境不同，并且可以包括基本上不含污染内源性物质的那些。

如本文使用的应用于核酸的术语“天然产生的”是指一种核酸，其在自然界的生物的细胞中发现，并且未经人类在实验室中进行有意的修饰。

多核苷酸或核酸序列的“片段”是指连续的核苷酸，其特别是本文一个实施方案的多核苷酸长度的至少15bp，至少30bp，至少40bp，至少50bp和/或至少60bp。特别地，多核苷酸的片段包含本文一个实施方案的多核苷酸的至少25个，更特别是至少50个，更特别是至少75个，更特别是至少100个，更特别是至少150个，更特别是至少200个，更特别是至少300个，更特别是至少400个，更特别是至少500个，更特别是至少600个，更特别是至少700个，更特别是至少800个，更特别是至少900个，更特别是至少1000个连续核苷酸。不受限制，本文的多核苷酸的片段可以用作PCR引物和/或探针，或用于反义基因沉默或RNAi。

如本文所用，术语“杂交”或在一定条件下杂交旨在描述杂交和洗涤的条件，在所述条件下彼此显著相同或同源的核苷酸序列保持彼此结合。该条件可以使得至少约70％、例如至少约80％、和例如至少约85％、90％或95％同一性的序列保持彼此结合。下文提供了低严格度、中等和高严格度杂交条件的定义。本领域技术人员可以通过例如Ausubel等人(1995,Current Protocols in Molecular Biology,John Wiley&Sons,sections 2,4,and6)所举例说明的那样以最少的实验来选择合适的杂交条件。另外，严格条件在Sambrook等人(1989,Molecular Cloning:A Laboratory Manual,2nd ed.,Cold Spring HarborPress,chapters 7,9,and 11)中描述。

“重组核酸序列”是通过使用实验室方法(例如分子克隆)将来自多于一个源的遗传物质组合在一起所生成的核酸序列，由此创造出或修饰出不是天然产生并且不能以其他方式在生物有机体中发现的核酸序列。

“重组DNA技术”是指用于制备重组核酸序列的分子生物学方法，例如描述于由Weigel和Glazebrook编辑的Laboratory Manuals，2002,Cold Spring Harbor Lab Press；和Sambrook等，1989Cold Spring Harbor,NY:Cold Spring Harbor Laboratory Press。

术语“基因”是指一种DNA序列，其包含可操作地连接到适当调控区域(例如启动子)的被转录为RNA分子(例如细胞中的mRNA)的区域。因此，基因可以包含几个可操作地连接的序列，诸如启动子、5’前导序列(包含例如参与翻译初始化的序列)、cDNA或基因组DNA的编码区、内含子、外显子和/或3’非翻译序列(包含例如转录终止位点)。

“多顺反子”是指可以在同一核酸分子内分别编码多于一个多肽的核酸分子，特别是mRNA。

“嵌合基因”是指通常不能在自然界的物种中发现的任何基因，特别是这样一种基因，其中核酸序列存在一个或多个部分在性质上彼此不相关联。例如，启动子在性质上与转录区的部分或全部或与另一调控区不相关联。术语“嵌合基因”应当被理解为包括表达构建体，其中启动子或转录调控序列被可操作地连接到一个或多个编码序列或反义(即有义链的反向互补链)或反向重复序列(有义和反义，由此RNA转录物在转录后形成双链RNA)。术语“嵌合基因”还包括通过组合一个或多个编码序列的部分以产生新基因而获得的基因。

“3’URT”或“3’非翻译序列”(也称为“3’未翻译区”或“3’末端”)是指在基因编码序列的下游发现的核酸序列，其包含例如转录终止位点和(在大多数但非全部的真核mRNA中)多聚腺苷酸化信号，例如AAUAAA或其变体。在转录终止后，mRNA转录物可以在多聚腺苷酸化信号的下游切去，并且可以添加poly(A)尾，其参与了mRNA向翻译位点例如细胞质的转运。

术语“引物”是指短的核酸序列，其被杂交到模板核酸序列并且被用于与该模板互补的核酸序列的聚合。

术语“可选择标记”是指在表达后能够被用来选择包括该可选择标记的一种或多种细胞的任何基因。以下描述了可选择标记的例子。本领域技术人员了解不同的抗生素、杀真菌剂、营养缺陷型或除草剂可选择标记可适用于不同的目标物种。

本发明还涉及编码如本文定义的多肽的核酸序列。

特别地，本发明还涉及编码上述多肽之一及其功能等同物的核酸序列(单链和双链DNA和RNA序列，例如cDNA、基因组DNA和mRNA)，其可以通过例如使用人工核苷酸类似物来获得。

本发明既涉及分离的核酸分子，其编码根据本发明的多肽或其生物学活性区段，又涉及核酸片段，其可用作例如鉴定或扩增根据本发明的编码核酸的杂交探针或引物。

本发明还涉及与本文具体公开的序列具有一定程度的“同一性”的核酸。两个核酸之间的“同一性”是指在每种情况下在核酸的整个长度上核苷酸的同一性。

两个核苷酸序列(同样适用于肽或氨基酸序列)之间的“同一性”是当产生这两个序列的比对时，核苷酸残基(或氨基酸残基)的数目的函数，或两个序列中相同的残基数目。相同的残基被定义为两个序列中在比对的给定位置的相同的残基。本文使用的序列同一性的百分比是从最佳比对中通过将两个序列之间相同的残基数除以最短序列中的残基总数并乘以100计算得到的。最佳比对是同一性百分比最高可能性的比对。可以将空位引入到一个或两个序列中的比对的一个或多个位置中以获得最佳比对。然后将这些空位考虑为用于计算序列同一性百分比的不相同的残基。用于确定氨基酸或核酸序列同一性百分比的比对可以使用计算机程序以及例如在互联网上可公开获得的计算机程序以多种方式实现。

特别地，可使用可从National Center for Biotechnology Information(美国国家生物技术信息中心)(NCBI)于http://www.ncbi.nlm.nih.gov/BLAST/bl2seq/wblast2.cgi获得的设定为默认参数的BLAST程序(Tatiana等，FEMS Microbiol Lett.,1999,174:247-250,1999)来获得蛋白或核酸序列的最佳比对并计算序列同一性的百分比。

在另一个例子中，同一性可以通过Informax公司(美国)的Vector NTI Suite 7.1程序使用Clustal方法(Higgins DG,Sharp PM.((1989)))通过以下设置来计算：

多重比对参数：

成对比对参数：

或者，同一性可以根据Chenna et al.(2003)，网页：http://www.ebi.ac.uk/Tools/clustalw/index.html#的方法和以下设置来确定：

本文提及的所有核酸序列(单链和双链DNA和RNA序列，例如cDNA和mRNA)可以以已知方式通过化学合成从核苷酸结构单元产生，例如通过双螺旋的各个重叠的互补核酸结构单元的片段缩合来实现。寡核苷酸的化学合成例如可以通过磷酰胺法(Voet,Voet,2ndedition,Wiley Press,New York,pages 896-897)以已知的方式进行。合成寡核苷酸的积累，和借助于DNA聚合酶的Klenow片段和连接反应的空位的填补，以及一般的克隆技术描述于Sambrook et al.(1989)，请参阅下文。

另外，根据本发明的核酸分子可以另外包含来自编码遗传区域的3'和/或5'末端的非翻译序列。

本发明进一步涉及与具体描述的核苷酸序列或其区段互补的核酸分子。

根据本发明的核苷酸序列使得可以产生可用于鉴定和/或克隆其他细胞类型和生物体中的同源序列的探针和引物。此类探针或引物通常包含在“严格”条件下(如本文其他部分所定义)与根据本发明的核酸序列的有义链或相应的反义链的至少约12个，优选至少约25个，例如约40、50或75个连续核苷酸杂交的核苷酸序列区域。

“同源”序列包括直系同源或旁系同源序列。鉴别直系同源物或旁系同源物的方法包括现有技术中已知且在本文中描述的系统发生学方法、序列相似性和杂交方法。

“旁系同源物”或旁系同源序列来源于基因复制，其产生具有相似序列和相似功能的两种或更多种基因。旁系同源物通常聚簇在一起并且通过在相关植物物种内基因的复制而形成。使用成对Blast分析或在基因家族的系统发生分析过程中使用程序诸如CLUSTAL在类似基因的组中发现旁系同源物。在旁系同源物中，共有序列可被鉴定为其特征在于相关基因中的序列并且具有基因的类似功能。

“直系同源物”或直系同源序列是彼此相似的序列，因为它们发现于由共同的祖先传下的物种中。例如，已知具有共同祖先的植物物种含有许多具有相似序列和功能的酶。例如通过使用CLUSTAL或BLAST程序构建一个物种的基因族的系统发生树，技术人员能够鉴定直系同源序列并预测直系同源物的功能。一种用于鉴定或确认同源序列间的相似功能的方法是通过比较过表达或缺乏(在基因敲除/敲减中)相关多肽的宿主细胞或生物体(如植物或微生物)中的转录物概况。技术人员能够理解，具有相似转录物概况的基因(具有大于50％调控的共同转录物，或具有大于70％调控的共同转录物，或大于90％调控的共同转录物)会具有相似的功能。通过使宿主细胞，生物体例如植物或微生物产生萜合酶蛋白，本文所述序列的同源物、旁系同源物、直系同源物以及任何其他变体预期以类似的方式发挥作用。

术语“可选择标记”是指在表达后能够被用来选择包括该可选择标记的一种或多种细胞的任何基因。以下描述了可选择标记的例子。本领域技术人员了解不同的抗生素、杀真菌剂、营养缺陷型或除草剂可选择标记可适用于不同的目标物种。

“分离的”核酸分子与存在于核酸天然来源中的其他核酸分子分离，并且如果通过重组技术生产，则可以基本上不含其他细胞材料或培养基，或者如果通过化学合成，则可以不含化学前体或其他化学物质。

可以借助于分子生物学的标准技术和根据本发明提供的序列信息来分离根据本发明的核酸分子。例如，可以使用具体公开的完整序列之一或其片段作为杂交探针和标准杂交技术(例如，描述于Sambrook,(1989))从合适的cDNA文库中分离cDNA。

另外，包含所公开的序列之一或其片段的核酸分子可以使用基于该序列构建的寡核苷酸引物，通过聚合酶链反应来分离。以此方式扩增的核酸可以克隆到合适的载体中，并可以通过DNA测序来表征。根据本发明的寡核苷酸也可以通过标准的合成方法，例如使用自动DNA合成仪制备。

根据本发明的核酸序列或其衍生物，这些序列的同源物或部分可以例如通过常规的杂交技术或PCR技术从其他细菌中，例如通过基因组或cDNA文库分离出来。这些DNA序列在标准条件下与根据本发明的序列杂交。

“杂交”是指多核苷酸或寡核苷酸在标准条件下结合几乎互补的序列的能力，而在这些条件下非互补配对者之间不发生非特异性结合。为此，序列可以是90～100％互补的。能够彼此特异性结合的互补序列的性质被用于例如Northern印迹或Southern印迹或PCR或RT-PCR中的引物结合。

保守区的短寡核苷酸有利地用于杂交。然而，也可能使用更长的本发明核酸片段或完整序列进行杂交。这些“标准条件”取决于所使用的核酸(寡核苷酸，更长的片段或完整序列)或用于杂交的核酸类型(DNA或RNA)而有所不同。例如，DNA:DNA杂交种的解链温度比相同长度的DNA:RNA杂交种低约10℃。

例如，根据特定核酸的不同，标准条件是指温度在42至58℃，在浓度为0.1至5 xSSC(1 X SSC＝0.15M NaCl，15mM柠檬酸钠，pH 7.2)的缓冲水溶液中，或另外在50％甲酰胺(例如42℃，5 x SSC，50％甲酰胺)的存在下。有利地，用于DNA:DNA杂交种的杂交条件是0.1×SSC，温度为约20℃至45℃，优选约30℃至45℃。对于DNA:RNA杂交种，杂交条件有利地为0.1×SSC，并且温度为约30℃至55℃，优选约45℃至55℃。这些所述的杂交温度是对于长度约100个核苷酸的核酸，以及在不存在甲酰胺的情况下G+C含量为50％的经计算的解链温度值的例子。DNA杂交的实验条件已在相关的遗传学教科书(例如Sambrook et al.,1989)中进行了描述，并且可以使用本领域技术人员已知的分子式来计算，例如取决于核酸的长度，杂交种的类型或G+C含量。本领域技术人员可以从以下教科书中获得有关杂交的更多信息：Ausubel et al.(eds),(1985),Brown(ed)(1991)。

“杂交”尤其可以在严格条件下进行。这样的杂交条件例如描述于Sambrook(1989)，或Current Protocols in Molecular Biology,John Wiley&Sons,N.Y.(1989),6.3.1-6.3.6。

如本文所用，术语“杂交或在一定条件下杂交”旨在描述杂交和洗涤的条件，在所述条件下彼此显著相同或同源的核苷酸序列保持彼此结合。该条件可以使得至少约70％，例如至少约80％和例如至少约85％、90％或95％同一性的序列保持彼此结合。本文提供了低严格度、中等和高严格度杂交条件的定义。

本领域技术人员可以通过例如Ausubel等人(1995,Current Protocols inMolecular Biology,John Wiley&Sons,sections 2,4,and 6)所举例说明的那样以最少的实验来选择合适的杂交条件。另外，严格条件在Sambrook等人(1989,Molecular Cloning:ALaboratory Manual,2nd ed.,Cold Spring Harbor Press,chapters 7,9,and 11)中描述。

如本文所用，所限定的低严格度条件如下。含有DNA的滤膜在含有35％甲酰胺，5xSSC，50mM Tris-HCl(pH 7.5)，5mM EDTA，0.1％PVP，0.1％Ficoll，1％BSA和500μg/ml变性鲑鱼精子DNA的溶液中于40℃预处理6小时。杂交在相同的溶液中进行，并进行以下修改：0.02％PVP，0.02％Ficoll，0.2％BSA，100μg/ml鲑鱼精子DNA，10％(wt/vol)硫酸葡聚糖，并使用5～20x10

如本文所用，所限定的中等严格度条件如下。含有DNA的滤膜在含有35％甲酰胺，5x SSC，50mM Tris-HCl(pH 7.5)，5mM EDTA，0.1％PVP，0.1％Ficoll，1％BSA和500μg/ml变性鲑鱼精子DNA的溶液中于50℃预处理7小时。杂交在相同的溶液中进行，并进行以下修改：0.02％PVP，0.02％Ficoll，0.2％BSA，100μg/ml鲑鱼精子DNA，10％(wt/vol)硫酸葡聚糖，并使用5～20x10

如本文所用，所限定的高严格度条件如下。含DNA的滤膜在由6x SSC，50mM Tris-HCl(pH 7.5)，1mM EDTA，0.02％PVP，0.02％Ficoll，0.02％BSA和500μg/ml变性鲑鱼精子DNA组成的缓冲液中于65℃下预杂交8小时至过夜。在含有100μg/ml变性鲑鱼精子DNA和5～20x10

如果上述条件不合适(例如，如用于种间杂交)，则可以使用本领域众所周知的其他低、中等和高严格度条件(例如，用于种间杂交)。

用于编码本发明多肽的核酸序列的检测试剂盒可以包括对编码该多肽的核酸序列具有特异性的引物和/或探针，以及使用该引物和/或探针来检测样品中编码该多肽的核酸序列的相关方案。此种检测试剂盒可用于确定植物、生物、微生物或细胞是否已被修饰，即是否已用编码多肽的序列转化。

为了测试根据本文一个实施方案的变体DNA序列的功能，将目标序列可操作地连接到可选择的或可筛选的标记基因，并且在使用微生物或原生质体进行的瞬时表达分析中或在稳定转化的植物中测试报告基因的表达。

本发明还涉及具体公开的或可衍生的核酸序列的衍生物。

因此，根据本发明的另外的核酸序列可以衍生自本文具体公开的序列，并且可以通过一个或几个(例如1至10个)核苷酸的一个或多个，例如1至20个，特别是1至15个或5至10个添加、取代、插入或缺失而与之不同，并且还编码具有所期望特性的多肽。

本发明还包括与具体陈述的序列相比，根据特定原始或宿主生物体的密码子使用而包含所谓的沉默突变或已被改变的核酸序列。

根据本发明的特定实施方案，可以制备变体核酸以使其核苷酸序列适应特定的表达系统。例如，如果氨基酸由特定的密码子编码，则已知细菌表达系统可更有效地表达多肽。由于遗传密码的简并性，多于一个密码子可以编码相同的氨基酸序列，多个核酸序列能够编码相同的蛋白或多肽，所有这些DNA序列均被涵盖在本文一个实施方案中。在适当的情况下，编码本文所述多肽的核酸序列可以被优化以增加在宿主细胞中的表达。例如，可以使用宿主特异性的密码子合成本文一个实施方案的核酸以改善表达。

本发明还涵盖本文描述的序列的天然存在的变体，例如剪接变体或等位基因变体。

等位基因变体在所衍生的氨基酸水平上，具有在整个氨基酸范围至少60％的同源性，优选至少80％的同源性，非常特别优选至少90％的同源性(关于氨基酸水平的同源性，应参考以上对于多肽给出的详细信息)。有利地，同源性可以在序列的部分区域上更高。

本发明还涉及可通过保守核苷酸取代(即，作为其结果，所讨论的氨基酸被具有相同电荷、大小、极性和/或溶解度的氨基酸取代)获得的序列。

本发明还涉及通过序列多态性从具体公开的核酸衍生的分子。由于天然等位基因变异，这种遗传多态性可能存在于来自不同群体的细胞或来自一个群体内的细胞中。等位基因变体还可包括功能等同物。这些自然变异通常会在基因的核苷酸序列中产生1～5％的变化。所述多态性可以导致本文公开的多肽的氨基酸序列的改变。等位基因变体还可包括功能等同物。

此外，衍生物也应理解为根据本发明的核酸序列的同源物，例如动物、植物、真菌或细菌的同源物，缩短的序列，编码和非编码DNA序列的单链DNA或RNA。例如，在DNA水平上，同源物在本文具体公开的序列中给定的整个DNA区域中具有至少40％，优选至少60％，特别优选至少70％，非常特别优选至少80％的同源性。

此外，衍生物应理解为例如与启动子的融合体。尽管不损害启动子的功能或功效，添加至所述核苷酸序列的启动子可以通过至少一种核苷酸交换、至少一种插入、倒置和/或缺失来修饰。而且，启动子的功效可以通过改变它们的序列来增加，或者可以与更有效的启动子甚至是不同属的生物体的启动子完全交换。

d.功能性多肽突变体的产生

此外，本领域技术人员熟悉用于产生功能性突变体的方法，也就是说，一种核苷酸序列，其编码多肽，该多肽与本文公开的任何与氨基酸相关的SEQ ID NO具有至少40％，45％，50％，55％，60％，65％，70％，75％，80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％的序列同一性；和/或由核酸分子编码，该核酸分子包含与本文公开的任何与核苷酸相关的SEQ ID NO具有至少70％序列同一性的核苷酸序列。

取决于所使用的技术，本领域技术人员可以将完全随机的或更有针对性的突变引入到基因或非编码核酸区域(例如对于调节表达很重要)中，并随后产生遗传文库。为此目的所需的分子生物学方法是技术人员已知的，例如描述于Sambrook and Russell,Molecular Cloning.3rd Edition,Cold Spring Harbor Laboratory Press 2001。

修饰基因并由此修饰由其编码的多肽的方法是本领域技术人员长期已知的，举例而言例如：

-位点特异性诱变，其中基因的单个或多个核苷酸以定向方式被替换(Trower MK(Ed.)1996；In vitro mutagenesis protocols.Humana Press,New Jersey)，

-饱和诱变，其中任何氨基酸的密码子都可以在基因的任何位点交换或添加(Kegler-Ebo DM,Docktor CM,DiMaio D(1994)Nucleic Acids Res 22:1593；BarettinoD,Feigenbutz M,Valcárel R,Stunnenberg HG(1994)Nucleic Acids Res 22:541；BarikS(1995)Mol Biotechnol 3:1)，

-易错聚合酶链反应，其中核苷酸序列被易错DNA聚合酶突变(Eckert KA,KunkelTA(1990)Nucleic Acids Res 18:3739)；

-SeSaM法(序列饱和法)，其中优选的交换被聚合酶阻止。Schenk et al.,Biospektrum,Vol.3,2006,277-279，

-突变株中的基因传代，其中例如由于DNA修复机制缺陷，核苷酸序列的突变率增加(Greener A,Callahan M,Jerpseth B(1996)An efficient random mutagenesistechnique using an E.coli mutator strain.In:Trower MK(Ed.)In vitromutagenesis protocols.Humana Press,New Jersey)，或

-DNA改组，其中形成并消化一组密切相关的基因，并将这些片段用作聚合酶链反应的模板，其中通过重复的链分离和重新结合，最终生成了全长的镶嵌基因(Stemmer WPC(1994)Nature 370:389；Stemmer WPC(1994)Proc Natl Acad Sci USA91:10747)。

使用所谓的定向进化(尤其描述于Reetz MT and Jaeger K-E(1999),TopicsCurr Chem 200:31；Zhao H,Moore JC,Volkov AA,Arnold FH(1999),Methods foroptimizing industrial polypeptides by directed evolution,In:Demain AL,DaviesJE(Ed.)Manual of industrial microbiology and biotechnology.American Societyfor Microbiology)，熟练的工人可以以定向的方式大规模生产功能性突变体。为此，在第一步中，首先，例如使用上文给出的方法，产生各自多肽的基因文库。基因文库以合适的方式表达，例如通过细菌或噬菌体展示系统来表达。

表达功能性突变体的宿主生物体的相关基因(其功能在很大程度上与所需的特性相对应)可以提交给另一个突变周期。突变和选择或筛选的步骤可以迭代地重复，直到本发明的功能性突变体具有足够程度的所需特性。使用该迭代过程，可以分阶段进行有限数量的突变，例如1、2、3、4或5个突变，并评估和选择它们对所研究活性的影响。然后可以以相同的方式将所选择的突变体进行进一步的突变步骤。这样，可以显著减少待研究的单个突变体的数量。

根据本发明的结果还提供了与相关多肽的结构和序列有关的重要信息，这是以靶向方式产生具有所需修饰特性的其他多肽所必需的。特别地，可以定义所谓的“热点”，即潜在地适合于通过引入靶向突变来修饰特性的序列区段。

也可以推导出有关氨基酸序列位置的信息，在该区域中可以发生可能对活性几乎没有影响的突变，并且可以将其指定为潜在的“沉默突变”。

e.表达本发明多肽的构建体

在本文语境中，以下定义适用：

“基因的表达”涵盖“异源表达”和“过表达”，并且涉及基因的转录和mRNA向蛋白质的翻译。过表达是指在转基因细胞或生物体中，以mRNA、多肽和/或酶活性水平测量的基因产物的产生超过了相似遗传背景的非转化细胞或生物体中的产生水平。

如本文所用，“表达载体”是指这样一种核酸分子，其使用分子生物学方法和重组DNA技术工程化以将外来或外源DNA递送到宿主细胞中。表达载体典型地包括正确转录核苷酸序列所需的序列。编码区通常编码目的蛋白，但是也可以编码RNA，例如反义RNA、siRNA等。

如本文所用，“表达载体”包括任何线性的或环状的重组载体，包括但不限于病毒载体、噬菌体和质粒。技术人员根据表达系统能够选择适合的载体。在一个实施方案中，表达载体包括本文实施方案的核酸，其可操作地连接到至少一个“调控序列”，其控制转录、翻译、起始和终止，例如转录启动子、操纵子或增强子，或mRNA核糖体结合位点，并且可选地包括至少一个选择标记。当调控序列功能性地涉及本文实施方案的核酸时，核苷酸序列是“可操作地连接的”。

如本文所用，“表达系统”涵盖在给定表达宿主的体内或体外共表达两种或更多种多肽所需的核酸分子的任何组合。各自的编码序列可以位于单个核酸分子或载体上，例如包含多个克隆位点的载体，或位于多顺反子核酸上，或者可以分布在两个或更多个物理上不同的载体上。

如本文所用，术语“进行扩增(amplifying)”和“扩增(amplification)”是指使用任何合适的扩增方法用于产生或检测天然表达的核酸的重组体，如下文详细描述的。例如，本发明提供用于扩增(例如，通过聚合酶链反应，PCR)天然表达的(例如，基因组DNA或mRNA)或本发明在体内、离体或体外的重组的核酸(例如cDNA)的方法和试剂(例如，特异性简并寡核苷酸引物对，寡聚dT引物)。

“调控序列”是指这样一种核酸序列，其确定本文实施方案的核酸序列的表达水平、并且能够调控可操作地连接到该调控序列的核酸序列的转录速率。调控序列包含启动子、增强子、转录因子、启动子元件等。

根据本发明，“启动子”、“具有启动子活性的核酸”或“启动子序列”应理解为是指这样一种核酸，当与要转录的核酸功能性连接时，其调节所述核酸的转录。“启动子”尤其是指一种核酸序列，其通过提供RNA聚合酶用的结合位点以及适合转录所需的其它因子，包括但不限于转录因子结合位点、抑制子和活化子蛋白结合位点，来控制编码序列的表达。术语启动子的含义还包括术语“启动子调控序列”。启动子调控序列可以包括可能影响转录、RNA加工或相关编码核酸序列的稳定性的上游和下游元件。启动子包括天然来源的和合成的序列。编码核酸序列通常位于启动子相对于以转录起始位点为起始的转录方向的下游。

在本文语境中，“功能性”或“可操作地”连接被理解为例如指具有调控序列的核酸之一的顺序排列。例如，具有启动子活性的序列，和待转录的核酸序列以及可选的其他调控元件(例如确保核酸转录的核酸序列)和例如终止子，该顺序排列的方式为使得每个调控元件都能在核酸序列转录后执行其功能。这不一定需要化学意义上的直接连接。遗传控制序列，例如增强子序列，甚至可以从更远的位置甚至从其他DNA分子上对目标序列发挥作用。优选的排列是这样的，其中待转录的核酸序列位于启动子序列的下游(即3'端)，从而使两个序列共价连接在一起。启动子序列和待重组表达的核酸序列之间的距离可以小于200个碱基对，或小于100个碱基对或小于50个碱基对。

除启动子和终止子外，还可以提及以下作为其他调控元件的例子：靶向序列，增强子，聚腺苷酸化信号，选择标记，扩增信号，复制起点等。合适的调节序列描述于例如Goeddel,Gene Expression Technology:Methods in Enzymology 185,Academic Press,San Diego,CA(1990)。

术语“组成型启动子”是指不受调控的启动子，其允许其可操作地连接的核酸序列的持续转录。

如本文所用，术语“可操作地连接”是指处于功能性关系的多核苷酸元件的连接。当核酸与另一核酸序列处于功能性关系时，那么该核酸是“可操作地连接”的。例如，如果启动子或者转录调控序列能够影响编码序列的转录，那么该启动子或者转录调控序列是可操作地连接到该编码序列的。可操作地连接意味着被连接的DNA序列通常是邻接的。与启动子序列有关的核苷酸序列相对于要被转化的植物可以是同源或异源来源的。所述序列还可以是完全或部分合成的。不管来源如何，与启动子序列有关的核酸序列将根据在结合到本文实施方案的多肽后所连接的启动子性质而表达或沉默。相关核酸在所有时间或替代地在特定时间在整个生物体中或在特定组织、细胞或细胞室中可以编码需要表达或抑制的蛋白。此种核苷酸序列特别地编码将所需表型性状赋予给由其改变或转化的宿主细胞或生物体的蛋白质。更特别地，相关的核苷酸序列导致在细胞或生物体中产生如本文定义的一种或多种目的产物。特别地，核苷酸序列编码具有如本文定义的酶活性的多肽。

本文如上所述的核苷酸序列可以是“表达盒”的一部分。术语“表达盒”和“表达构建体”同义使用。(优选的重组)表达构建体包含这样的核苷酸序列，其编码根据本发明的多肽并且在调节核酸序列的遗传控制之下。

在根据本发明应用的方法中，表达盒可以是“表达载体”，特别是重组表达载体的一部分。

根据本发明，“表达单位”应理解为是指具有表达活性的核酸，其包含如本文所定义的启动子，并且在与待表达的核酸或基因功能性连接后调节表达，即所述核酸或所述基因的转录和翻译。因此在这方面也被称为“调节核酸序列”。除启动子外，还可以存在其他调节元件，例如增强子。

根据本发明，“表达盒”或“表达构建体”应理解为在功能上与要表达的核酸或要表达的基因连接的表达单元。因此，与表达单元相反，表达盒不仅包含调节转录和翻译的核酸序列，而且还包含由于转录和翻译而作为蛋白质表达的核酸序列。

在本发明的语境中，术语“表达”或“过表达”描述了微生物中一种或多种由相应DNA编码的多肽的细胞内活性的产生或增加。为此，例如可以将基因导入到生物体中，用另一个基因替代现有基因，增加基因的拷贝数，使用强启动子或使用编码具有高活性的相应多肽的基因。可选地，这些措施可以组合。

优选地，根据本发明的此类构建体包含各自编码序列5'上游的启动子和3'下游的终止子序列，以及可选地其他常见的调控元件，在每种情况下均与编码序列可操作地连接。

根据本发明的核酸构建体特别地包含编码多肽的序列，该多肽例如衍生自如本文所述的氨基酸相关的SEQ ID NO或其反向互补序列，或其衍生物和同源物，并且已经与一个或多个调节信号可操作地或功能性连接，用于有利地控制例如增加基因表达。

除了这些调控序列之外，这些序列的天然调控可能仍存在于实际的结构基因之前，并且可选地可能已经进行了遗传修饰，因此天然调控已被关闭，基因的表达得到了增强。然而，核酸构建体也可以具有更简单的构建，即，在编码序列之前没有插入额外的调节信号，并且具有调节作用的天然启动子尚未去除。相反，天然调节序列被突变，使得不再发生调节并且基因表达增加。

优选的核酸构建体有利地还包含与启动子功能性连接的一个或多个已经提及的“增强子”序列，该序列使得增强核酸序列的表达成为可能。还可以在DNA序列的3'末端插入其他有利的序列，例如其他调控元件或终止子。根据本发明的核酸的一个或多个拷贝可以存在于构建体中。在该构建体中，还可以可选地存在其他标记物，例如与营养缺陷性或抗生素抗性互补的基因，以便选择该构建体。

合适的调控序列的例子存在于启动子中，例如cos、tac、trp、tet、trp-tet、lpp、lac、lpp-lac、lacI

为了在宿主生物体中表达，将核酸构建体有利地插入到载体，例如质粒或噬菌体中，这使得基因在宿主中的最佳表达成为可能。除了质粒和噬菌体，载体还应理解为是本领域技术人员已知的所有其他载体，即例如病毒，例如SV40、CMV、杆状病毒和腺病毒、转座子、IS元件、噬粒、粘粒和线性或环状DNA或人工染色体。这些载体能够在宿主生物体中自主复制或通过染色体复制。这些载体是本发明的进一步发展。二元或cpo整合载体也是适用的。

合适的质粒是例如在大肠杆菌pLG338、pACYC184、pBR322、pUC18、pUC19、pKC30、pRep4、pHS1、pKK223-3、pDHE19.2、pHS2、pPLc236、pMBL24、pLG200、pUR290、pIN-III

在载体的进一步开发中，包含本发明的核酸构建体或本发明的核酸的载体也可以有利地以线性DNA的形式引入到微生物中并通过异源或同源重组整合到宿主生物体的基因组中。该线性DNA可以由线性化的载体例如质粒组成，或者仅由本发明的核酸构建体或核酸组成。

为了在生物体中异源基因的最佳表达，有利的是修饰核酸序列以匹配生物体中使用的特定“密码子使用”。“密码子使用”可以通过对所讨论的生物体的其他已知基因的计算机评估来容易地确定。

根据本发明的表达盒通过将合适的启动子融合到合适的编码核苷酸序列和终止子或聚腺苷酸化信号来产生。为此目的使用常规的重组和克隆技术，例如描述于T.Maniatis,E.F.Fritsch and J.Sambrook,Molecular Cloning:A Laboratory Manual,Cold Spring Harbor Laboratory,Cold Spring Harbor,NY(1989)和T.J.Silhavy,M.L.Berman and L.W.Enquist,Experiments with Gene Fusions,Cold Spring HarborLaboratory,Cold Spring Harbor,NY(1984)和Ausubel,F.M.et al.,Current Protocolsin Molecular Biology,Greene Publishing Assoc.and Wiley Interscience(1987)。

为了在合适的宿主生物体中表达，将重组核酸构建体或基因构建体有利地插入到宿主特异性载体中，这使得基因在宿主中的最佳表达成为可能。载体是技术人员众所周知的，并且可以在例如“cloning vectors”(Pouwels P.H.et al.,Ed.,Elsevier,Amsterdam-New York-Oxford,1985)中找到。

本文实施方案的替代实施方案提供了一种“改变宿主细胞中的基因表达”的方法。例如，在某些语境下(例如，暴露于一定温度或培养条件下)，在宿主细胞或宿主生物体中可以增强或过表达或诱导本文实施方案的多核苷酸。

本文提供的多核苷酸的表达的改变还会产生异位表达，其是在改变的以及在对照或野生型生物体中的一种不同的表达模式。表达的改变是由本文一个实施方案的多肽与外源性或内源性调节剂的接触而发生的或者是由于多肽的化学修饰导致的。该术语还指本文实施方案的多核苷酸的改变的表达模式，其被改变至低于检测水平或者完全被抑制活性。

本文提供的一个实施方案还为编码本文提供的多肽或变体多肽的分离的、重组的或合成的多核苷酸。

在一个实施方案中，多种编码多肽的核酸序列在单一宿主中共表达，特别是在不同启动子的控制下。在另一个实施方案中，多种编码多肽的核酸序列可以存在于单个转化载体上，或者可以使用分离的载体并选择包含两个嵌合基因的转化体同时进行共转化。类似地，一种或多种多肽编码基因可以与其他嵌合基因一起在单一植物、细胞、微生物或生物体中表达。

f.适用于本发明的宿主

取决于语境，术语“宿主”可以指野生型宿主或经遗传改变的重组宿主或两者。

原则上，所有的原核或真核生物都可以被认为是根据本发明的核酸或核酸构建体的宿主或重组宿主生物体。

使用根据本发明的载体，可以生产重组宿主，其例如可以用至少一种根据本发明的载体转化，并可以用于生产根据本发明的多肽。有利地，将如上所述的根据本发明的重组构建体引入到合适的宿主系统中并表达。优选地，使用本领域技术人员已知的普通克隆和转染方法，例如共沉淀、原生质体融合、电穿孔、逆转录病毒转染等，以在各自的表达系统中表达所述核酸。合适的系统描述于Current Protocols in Molecular Biology,F.Ausubelet al.,Ed.,Wiley Interscience,New York 1997，或Sambrook et al.MolecularCloning:A Laboratory Manual.2nd edition,Cold Spring Harbor Laboratory,ColdSpring Harbor Laboratory Press,Cold Spring Harbor,NY,1989。

有利地，诸如细菌、真菌或酵母的微生物被用作宿主生物体。有利地，使用革兰氏阳性或革兰氏阴性细菌，优选肠杆菌科(Enterobacteriaceae)，假单胞菌科(Pseudomonadaceae)，根瘤菌科(Rhizobiaceae)，链霉菌科(Streptomycetaceae)，链球菌科(Streptococcaceae)或诺卡氏菌科(Nocardiaceae)的细菌，特别优选埃希氏菌属(Escherichia)，假单胞菌属(Pseudomonas)，链霉菌属(Streptomyces)，乳球菌属(Lactococcus)，诺卡氏菌(Nocardia)，伯克霍尔德氏菌属(Burkholderia)，沙门氏菌属(Salmonella)，农杆菌属(Agrobacterium)，艰难梭菌(Clostridium)或红球菌属(Rhodococcus)的细菌。大肠杆菌(Escherichia coli)属和种是非常特别优选的。此外，在α-变形菌(alpha-Proteobacteria)、β-变形菌(beta-Proteobacteria)或γ-变形菌(gamma-Proteobacteria)组中发现了其他有利的细菌。有利地，诸如酵母属(Saccharomyces)或毕赤酵母(Pichia)家族的酵母也是合适的宿主。

或者，整个植物或植物细胞可以用作天然或重组宿主。作为非限制性例子，可以提及以下植物或自其衍生的细胞：烟草属(Nicotiana)，特别是本氏烟草(Nicotianabenthamiana)和普通烟草(Nicotiana tabacum)(tobacco)；以及拟南芥属(Arabidopsis)，特别是阿拉伯芥(Arabidopsis thaliana)。

取决于宿主生物体，根据本发明的方法中使用的生物体以本领域技术人员已知的方式生长或培养。培养可以分批、半分批或连续进行。营养物可以在发酵开始时给予，也可以稍后，半连续或连续地提供。这也在下面更详细地描述。

g.根据本发明的多肽的重组生产

本发明进一步涉及重组生产根据本发明的多肽或其功能性生物学活性片段的方法，其中培养产生多肽的微生物，可选地通过施加至少一种诱导基因表达的诱导剂来诱导多肽的表达，并从培养物中分离这些多肽。如果需要，多肽也可以这种方式以工业规模生产。

根据本发明产生的微生物可以分批法或补料分批法或重复补料分批法连续或不连续培养。已知培养方法的概述可在Chmiel的教科书(Bioprozesstechnik 1.Einführungin die Bioverfahrenstechnik[Bioprocess technology 1.Introduction tobioprocess technology](Gustav Fischer Verlag,Stuttgart,1991))或在Storhas的教科书(Bioreaktoren und periphere Einrichtungen[Bioreactors and peripheralequipment](Vieweg Verlag,Braunschweig/Wiesbaden,1994))中找到。

所使用的培养基必须适当地满足各个菌株的要求。在美国细菌学学会(AmericanSociety for Bacteriology(Washington D.C.,USA,1981))的手册“Manual of Methodsfor General Bacteriology”中给出了各种微生物的培养基的描述。

可以根据本发明使用的这些培养基通常包含一种或多种碳源、氮源、无机盐、维生素和/或微量元素。

优选的碳源是糖，例如单糖、二糖或多糖。很好的碳源是例如葡萄糖，果糖，甘露糖，半乳糖，核糖，山梨糖，核酮糖，乳糖，麦芽糖，蔗糖，棉子糖，淀粉或纤维素。糖也可以通过复杂的化合物(例如糖蜜)或糖精制的其他副产品添加到培养基中。添加不同碳源的混合物也是有利的。其他可能的碳源是油和脂，例如大豆油，葵花籽油，花生油和椰子油，脂肪酸例如棕榈酸，硬脂酸或亚油酸，醇例如甘油，甲醇或乙醇，和有机酸，例如乙酸或乳酸。

氮源通常是有机或无机氮化合物或包含这些化合物的材料。氮源的例子包括氨气或铵盐，例如硫酸铵，氯化铵，磷酸铵，碳酸铵或硝酸铵，硝酸盐，尿素，氨基酸或复合氮源，例如玉米浆，大豆粉，大豆蛋白，酵母提取物，肉提取物等。氮源可以单独使用或混合使用。

可以存在于培养基中的无机盐化合物包括钙，镁，钠，钴，钼，钾，锰，锌，铜和铁的氯化物、磷或硫酸盐。

无机含硫化合物，例如硫酸盐，亚硫酸盐，连二亚硫酸盐，四硫酸盐，硫代硫酸盐，硫化物，以及有机硫化合物，例如硫醇(mercaptans)和巯类(thiols)，可用作硫源。

磷酸、磷酸二氢钾或磷酸氢二钾或相应的含钠盐可用作磷源。

可以将螯合剂添加到培养基中，以将金属离子保持在溶液中。特别合适的螯合剂包括二羟基苯酚，例如儿茶酚或原儿茶酸酯，或有机酸，例如柠檬酸。

根据本发明使用的发酵培养基通常还包含其他生长因子，例如维生素或生长促进剂，其包括例如生物素，核黄素，硫胺素，叶酸，烟酸，泛酸和吡哆醇(pyridoxine)。生长因子和盐通常源自复杂培养基的成分，例如酵母提取物，糖蜜，玉米浆等。此外，可以将合适的前体添加到培养基中。化合物在培养基中的确切组成在很大程度上取决于相应的实验，并针对每种具体情况分别确定。有关培养基优化的信息可以在教科书"AppliedMicrobiol.Physiology,A Practical Approach"(Ed.P.M.Rhodes,P.F.Stanbury,IRLPress(1997)p.53-73,ISBN 0 19 963577 3)中找到。生长培养基也可以从商业供应商获得，例如Standard 1(Merck)或BHI(脑心浸液，DIFCO)等。

通过加热(在1.5bar和121℃下20分钟)或通过无菌过滤对培养基的所有成分进行灭菌。这些成分可以一起消毒，也可以根据需要单独消毒。培养基的所有成分都可以在培养开始时给予，也可以连续或分批添加。

培养物的温度通常在15℃至45℃之间，优选25℃至40℃，并且在实验过程中可以改变或保持恒定。介质的pH应在5至8.5的范围内，优选为7.0左右。生长期间的pH值可以通过添加碱性化合物(例如氢氧化钠，氢氧化钾，氨或氨水)或酸性化合物(例如磷酸或硫酸)来控制。消泡剂例如脂肪酸聚乙二醇酯可用于控制发泡。为了维持质粒的稳定性，可以向培养基中添加合适的选择性物质例如抗生素。为了维持有氧条件，将氧气或含氧气体混合物(例如环境空气)供入培养物中。培养物的温度通常在20℃至45℃的范围内。继续培养直至形成最大量的所期望产物。通常会在10到160个小时内达到此目标。

然后将发酵液进一步处理。根据需要，可以通过分离技术，例如离心、过滤、倾析或这些方法的组合，将生物质完全或部分地从发酵液中除去，或者可以完全留在其中。

如果多肽没有在培养基中分泌，那么细胞也可以被裂解，并且可以通过用于分离蛋白质的已知方法从裂解物中获得产物。可以可选地通过高频超声，高压例如在高压细胞裂解机(French press)中，通过渗透，通过去污剂、裂解酶或有机溶剂的作用，通过均化器或通过上述几种方法的组合来破坏细胞。

可以通过已知的色谱技术，例如分子筛色谱(凝胶过滤)，例如Q-琼脂糖色谱，离子交换色谱和疏水色谱，以及其他常规技术，例如超滤、结晶、盐析、渗析和天然凝胶电泳来纯化多肽。合适的方法描述于例如Cooper,T.G.,Biochemische Arbeitsmethoden[Biochemical processes],Verlag Walter de Gruyter,Berlin,New York，或Scopes,R.,Protein Purification,Springer Verlag,New York,Heidelberg,Berlin。

为了分离重组蛋白，使用载体系统或寡核苷酸可能是有利的，所述载体系统或寡核苷酸通过限定的核苷酸序列延长cDNA，并因此编码改变的多肽或融合蛋白，其例如用于更容易的纯化。这种类型的合适修饰例如是充当锚的所谓“标签”，例如可以被识别为抗体抗原的被称为六-组氨酸锚或表位的修饰(例如，描述于Harlow,E.and Lane,D.,1988,Antibodies:A Laboratory Manual.Cold Spring Harbor(N.Y.)Press)。这些锚可以用于将蛋白质连接至固体载体，例如聚合物基质，其可以例如用作色谱柱中的填料，或者可以用于微量滴定板或其他载体上。

同时，这些锚也可用于识别蛋白质。为了识别蛋白质，还可以使用通常的标记物，例如荧光染料，酶标记物(与底物反应后形成可检测的反应产物)，或放射性标记物，单独使用或与锚结合使用以衍生化蛋白质。

h.多肽的固定化

根据本发明的酶或多肽可以在本文描述的方法中以游离形式或固定化而使用。固定化酶是固定在惰性载体上的酶。合适的载体材料和固定在其上的酶从EP-A-1149849，EP-A-1069183和DE-OS100193773以及从其中引用的参考文献中已知。在这方面，参考这些文件的全部公开内容。合适的载体材料包括例如粘土，粘土矿物，例如高岭石，硅藻土，珍珠岩，二氧化硅，氧化铝，碳酸钠，碳酸钙，纤维素粉末，阴离子交换剂材料，合成聚合物，例如聚苯乙烯，丙烯酸树脂，酚醛树脂，聚氨酯和聚烯烃，例如聚乙烯和聚丙烯。为了制备负载的酶，通常以细分的颗粒形式，优选多孔形式使用载体材料。载体材料的粒径通常不大于5mm，特别是不大于2mm(粒径分布曲线)。类似地，当使用脱氢酶作为全细胞催化剂时，可以选择游离形式或固定形式。载体材料例如为海藻酸钙和角叉菜胶。酶和细胞也可以直接与戊二醛交联(与CLEAs交联)。相应的和其他固定化技术描述于例如J.Lalonde and A.Margolin"Immobilization of Enzymes"in K.Drauz and H.Waldmann,Enzyme Catalysis inOrganic Synthesis 2002,Vol.III,991-1032,Wiley-VCH,Weinheim中。Rehm et al.(Ed.)Biotechnology,2nd Edn,Vol 3,Chapter 17,VCH,Weinheim给出了用于进行根据本发明的方法的生物转化和生物反应器的进一步信息。

i.本发明生物催化生产方法的反应条件

本发明的反应可以在体内或体外条件下进行。

存在于本发明的方法或上文定义的多步方法的单个步骤中的至少一种多肽/酶可以天然存在于活细胞中，或于收获的细胞(即在体内条件下)中，死细胞中，透化细胞中，粗细胞提取物中，纯化提取物中，或以基本纯净或完全纯净的形式(即在体外条件下)，重组产生一种或多种酶。所述至少一种酶可以以溶液形式存在或以固定在载体上的酶形式存在。一种或几种酶可以同时以可溶性和/或固定化形式存在。

根据本发明的方法可以在本领域技术人员已知的普通反应器中进行，并且可以在不同的规模范围内进行，例如从实验室规模(几毫升到几十升反应体积)到工业规模(几升到数千立方米反应体积)。如果多肽以通过无生命的、可选地透化的细胞包封的形式，以或多或少纯化的细胞提取物的形式或以纯化的形式使用，则可以使用化学反应器。化学反应器通常允许控制至少一种酶的量，至少一种底物的量，pH，温度和反应介质的循环。当活细胞中存在至少一种多肽/酶时，该过程将是发酵。在这种情况下，生物催化生产将在生物反应器(发酵罐)中进行，其中对于活细胞合适的生存条件必需的参数(例如，具有营养的培养基，温度，通气，有氧或无氧或其他气体，抗生素等)可以控制。本领域技术人员熟悉化学反应器或生物反应器，例如使用将化学或生物技术方法从实验室规模扩大到工业规模或优化工艺参数的程序，这些方法在文献中也有广泛描述(有关生物技术方法，请参见例如Crueger und Crueger,Biotechnologie–Lehrbuch der angewandten Mikrobiologie,2.Ed.,R.Oldenbourg Verlag,München,Wien,1984)。

包含至少一种酶的细胞可以通过物理或机械方式例如超声或射频脉冲，高压细胞裂解机(French press)或化学方式例如在培养基中存在的低渗介质、裂解酶和去污剂或这些方法的组合来渗透。洗涤剂的例子是洋地黄毒苷，正十二烷基麦芽糖苷，辛基糖苷，

代替活细胞，也可以将含有所需生物催化剂的非活细胞的生物质应用于本发明的生物转化反应。

如果固定了至少一种酶，则将其如上所述连接至惰性载体。

转化反应可以分批、半分批或连续进行。反应物(和可选的营养物)可以在反应开始时提供，或者可以随后半连续或连续地提供。

根据特定的反应类型，本发明的反应可以在水性、水性-有机或非水性反应介质中进行。

水性或水性-有机介质可包含合适的缓冲液，以将pH值调整为5至11，例如6至10。

在水性-有机介质中，可以使用与水可混溶、部分混溶或不混溶的有机溶剂。合适的有机溶剂的非限制性例子在下面列出。进一步的例子是一元或多元，芳族或脂族醇，特别是多元脂族醇，如甘油。

非水介质可以包含基本上不含水，即，将包含少于约1重量％或0.5重量％的水。

生物催化方法也可以在有机非水介质中进行。作为合适的有机溶剂，可以提及具有例如5至8个碳原子的脂族烃，例如戊烷，环戊烷，己烷，环己烷，庚烷，辛烷或环辛烷；芳族烃，例如苯，甲苯，二甲苯，氯苯或二氯苯，脂族无环和醚，例如二乙醚，甲基叔丁基醚，乙基叔丁基醚，二丙基醚，二异丙醚，二丁基醚；或它们的混合物。

反应物/底物的浓度可以适应于最佳反应条件，这可以取决于所应用的特定酶。例如，初始底物浓度可以为0.1至0.5M，例如10至100mM。

反应温度可以适应于最佳反应条件，这可以取决于所应用的特定酶。例如，该反应可以在0至70℃的温度下进行，例如20至50或25至40℃。反应温度的例子是约30℃，约35℃，约37℃，约40℃，约45℃，约50℃，约55℃和约60℃。

该工艺可以继续进行直到在底物和随后的产物之间达到平衡为止，但是可以更早地停止。通常的工艺时间为1分钟至25小时，特别是10分钟至6小时，例如为1小时至4小时，特别是1.5小时至3.5小时。这些参数是合适的工艺条件的非限制性例子。

如果宿主是转基因植物，则可以提供最佳的生长条件，例如最佳的光照、水和营养条件。

用于进行乙酸补身烷基酯化合物的制备的特定反应条件如下。在20至35℃和4至7的pH值下孵育的水性环境中，乙酰转移酶可以作为经纯化的多肽或全细胞系统存在。底物浓度可以在10至100mM之间变化。

k.产品分离

本发明的方法可以进一步包括回收终产物或中间产物的步骤，所述终产物或中间产物可选地为立体异构体或对映异构体的基本纯净的形式。术语“回收”包括从培养基或反应介质中提取、收获、分离或纯化化合物。化合物的回收可以根据本领域已知的任何常规分离或纯化方法进行，包括但不限于用常规树脂(例如，阴离子或阳离子交换树脂，非离子吸附树脂等)处理，用常规吸附剂(例如，活性炭，硅酸，硅胶，纤维素，氧化铝等)处理，pH值的改变，溶剂萃取(例如，使用常规溶剂，例如醇，乙酸乙酯，己烷等)，蒸馏，渗析，过滤，浓缩，结晶，重结晶，pH调节，冻干等。

经分离产物的身份和纯度可以通过已知技术确定，例如高效液相色谱(HPLC)，气相色谱(GC)，光谱学(例如IR，UV，NMR)，着色方法，TLC，NIRS，酶或微生物测定(参见例如：Patek et al.(1994)Appl.Environ.Microbiol.60:133-140；Malakhova et al.(1996)Biotekhnologiya 1127-32；und Schmidt et al.(1998)Bioprocess Engineer.19:67-70.Ullmann's Encyclopedia of Industrial Chemistry(1996)Bd.A27,VCH:Weinheim,S.89-90,S.521-540,S.540-547,S.559-566,575-581und S.581-587；Michal,G(1999)Biochemical Pathways:An Atlas of Biochemistry and Molecular Biology,JohnWiley and Sons；Fallon,A.et al.(1987)Applications of HPLC in Biochemistry in:Laboratory Techniques in Biochemistry and Molecular Biology,Bd.17.)。

可以将本文所述的任何方法生产的环状萜烯化合物转化成衍生物，例如但不限于烃，酯，酰胺，糖苷，醚，环氧化物，醛，酮，醇，二醇，缩醛或缩酮。萜烯化合物衍生物可以通过化学方法获得，例如但不限于氧化，还原，烷基化，酰化和/或重排。或者，萜烯化合物衍生物可以通过使用生化方法通过使萜烯化合物与酶接触而获得，所述酶例如但不限于氧化还原酶，单加氧酶，双加氧酶，转移酶。可以使用分离的酶，来自裂解细胞的酶在体外进行生化转化，也可以使用全细胞在体内进行生化转化。

l.乙酸补身烷基醇的发酵产生

本发明还涉及用于发酵产生乙酸补身烷基酯的方法。

根据本发明使用的发酵可以例如在搅拌的发酵罐、鼓泡塔和回路反应器中进行。有关可能的方法类型的全面概述，包括搅拌器类型和几何设计，请参见“Chmiel:Bioprozesstechnik:Einfuhrung in die Bioverfahrenstechnik,Band 1”。在本发明的方法中，可用的典型变型是本领域技术人员已知的或例如在“Chmiel,Hammes and Bailey:Biochemical Engineering”中解释的以下变型，例如分批、补料分批，重复补料分批进行或连续发酵，有或没有回收生物质。取决于生产应变，可以进行空气，氧气，二氧化碳，氢气，氮气或适当的气体混合物的喷射，以实现良好的收率(YP/S)。

要使用的培养基必须以适当的方式满足特定菌株的要求。在美国细菌学学会(American Society for Bacteriology(Washington D.C.,USA,1981))的手册“Manual ofMethods for General Bacteriology”中给出了各种微生物的培养基的描述。

可以根据本发明使用的这些培养基通常包含一种或多种碳源、氮源、无机盐、维生素和/或微量元素。

优选的碳源是糖，例如单糖、二糖或多糖。非常好的碳源是例如葡萄糖，果糖，甘露糖，半乳糖，核糖，山梨糖，核酮糖，乳糖，麦芽糖，蔗糖，棉子糖，淀粉或纤维素。糖也可以通过复杂的化合物(例如糖蜜)或糖精制的其他副产物添加到培养基中。添加各种碳源的混合物也是有利的。碳的其他可能来源是油和脂，例如大豆油，葵花籽油，花生油和椰子油，脂肪酸例如棕榈酸，硬脂酸或亚油酸，醇例如甘油，甲醇或乙醇，和有机酸，例如乙酸或乳酸。

氮源通常是有机或无机氮化合物或含有这些化合物的材料。氮源的例子包括氨气或铵盐，例如硫酸铵，氯化铵，磷酸铵，碳酸铵或硝酸铵，硝酸盐，尿素，氨基酸或复合氮源，例如玉米浆，大豆粉，大豆蛋白，酵母提取物，肉提取物等。氮源可以单独使用或混合使用。

可以存在于培养基中的无机盐化合物包括钙，镁，钠，钴，钼，钾，锰，锌，铜和铁的氯化物，磷酸盐或硫酸盐。

无机含硫化合物，例如硫酸盐，亚硫酸盐，连二亚硫酸盐，四硫酸盐，硫代硫酸盐，硫化物，以及有机硫化合物，例如硫醇(mercaptans)和巯类(thiols)，可用作硫源。

磷酸、磷酸二氢钾或磷酸氢二钾或相应的含钠盐可用作磷源。

可以将螯合剂添加到培养基中，以将金属离子保持在溶液中。特别合适的螯合剂包括二羟基苯酚，例如儿茶酚或原儿茶酸酯，或有机酸，例如柠檬酸。

根据本发明使用的发酵培养基还可包含其他生长因子，例如维生素或生长促进剂，其包括例如生物素，核黄素，硫胺素，叶酸，烟酸，泛酸和吡哆醇。生长因子和盐通常来自培养基的复杂成分，例如酵母提取物，糖蜜，玉米浆等。另外，可以将合适的前体添加到培养基中。化合物在培养基中的精确组成在很大程度上取决于特定的实验，必须针对每种特定情况分别确定。有关培养基优化的信息可以在教科书"Applied Microbiol.Physiology,APractical Approach"(1997)中找到。生长培养基也可以从商业供应商那里获得，例如Standard 1(Merck)或BHI(脑心浸液，DIFCO)等。。

通过加热(在1.5bar和121℃下20分钟)或通过无菌过滤对培养基的所有成分进行灭菌。这些成分可以一起消毒，也可以根据需要单独消毒。培养基的所有成分都可以在生长开始时给予，或者可以选择连续添加或分批添加。

培养物的温度通常在15℃至45℃之间，优选25℃至40℃，并且可以在实验期间保持恒定或可以变化。介质的pH值应在5至8.5的范围内，优选7.0左右。生长期间的pH值可以通过添加碱性化合物(例如氢氧化钠，氢氧化钾，氨或氨水)或酸性化合物(例如磷酸或硫酸)来控制。消泡剂例如脂肪酸聚乙二醇酯可用于控制发泡。为了维持质粒的稳定性，可以向培养基中添加具有选择性作用的合适物质例如抗生素。为了维持有氧条件，将氧气或含氧气体混合物(例如环境空气)供入培养物中。培养物的温度通常为20℃至45℃。继续培养直至形成最大量的所期望产物。通常会在1到160个小时内达到此目标。

本发明的方法可以进一步包括回收所述乙酸补身烷基酯的步骤。

术语“回收”包括从培养基中提取、收获、分离或纯化化合物。化合物的回收可以根据本领域已知的任何常规分离或纯化方法进行，包括但不限于用常规树脂(例如，阴离子或阳离子交换树脂，非离子吸附树脂等)处理，用常规吸附剂(例如，活性炭，硅酸，硅胶，纤维素，氧化铝等)处理，pH值的改变，溶剂萃取(例如，使用常规溶剂，例如醇，乙酸乙酯，己烷等)，蒸馏，渗析，过滤，浓缩，结晶，重结晶，pH调节，冻干等。

在预期的分离之前，可以除去发酵液的生物质。去除生物质的方法是本领域技术人员已知的，例如过滤、沉降和浮选。因此，可以例如通过离心机、分离器、倾析器、过滤器或在浮选设备中去除生物质。为了最大程度地回收有价值的产品，通常建议洗涤生物质，例如以渗滤的形式。方法的选择取决于发酵液中生物质的含量和生物质的性质，以及生物质与有价值产品的相互作用。

在一个实施方案中，可以将发酵液灭菌或巴氏灭菌。在另一个实施方案中，将发酵液浓缩。根据需要，该浓缩可以分批或连续进行。应该选择压力和温度范围，使得首先不会发生产品损坏，其次需要最小化设备和能源的使用。多级蒸发的压力和温度水平的熟练选择尤其可以节省能源。

以下实施例仅是说明性的，并不意味着限制本文所述的权利要求和实施方案的范围。

在考虑了本文提供的公开内容之后，对于本领域技术人员而言将立即变得显而易见的多种可能的变型方案也落入本发明的范围内。

材料：

除非另有说明，否则本文使用的所有化学和生物化学材料以及微生物或细胞均为可商购的产品。

除非另有说明，否则重组蛋白是通过标准方法克隆和表达的，所述方法例如描述于Sambrook,J.,Fritsch,E.F.and Maniatis,T.,Molecular cloning:A LaboratoryManual,2

气相色谱质谱法(GC-MS)

Agilent Intuvo 9000系列气相色谱系统配备DB-5MS UI色谱柱(10m×0.25mm×0.25μm膜厚(定制色谱柱，由Agilent Technologies Inc,Santa Clara,CA制)，气相色谱通过1:1的Detector Splitter Chip(G4588-60502,Agilent Technologies Inc,SantaClara,CA)与两个检测器相连。，第一个检测器是Agilent 5977B系列质谱仪，而第二个检测器是标准的Intuvo 9000火焰离子化检测器(FID)。载气为氦气，流速为2.5ml/min。进样器以分流(1:100)模式进样，进样器温度设置为240℃。烘箱温度从150℃(保持0.1分钟)编程为以40℃/min的速度升至240℃，然后以180℃/min的温度升至325℃，并保持0.5分钟)。

乙酰基转移酶构成了遗传上多样化的一类酶，已知的代表超过8,000种(PFAM数据库：PF02458转移酶家族)。尽管被乙酰基转移酶接受为底物的分子的库很大，但据报道没有一种接受倍半萜醇作为底物。为了鉴定能够乙酰化补身烷型倍半萜醇的乙酰基转移酶，基于以下原理从数千种已知的源自植物、真菌和细菌的乙酰基转移酶中选择了五十四种(表1)：

从植物乙酰基转移酶BAHD家族中获知的五个进化枝中的两种——进化枝3和5——的鉴定出的成员是用乙酰辅酶A作为酰基供体对生物碱和萜类化合物进行乙酰化(Curr Opin Plant Biol.2006,9(3):331-40)。另外，来自进化枝3和5的乙酰基转移酶使用的一些底物是大体积多环的并且带有空间位阻醇基团作为酰基受体(BMC Genomics 2011,12:236；Curr Opin Plant Biol.2006,9:331-40；Elife.2017Mar14；6:e23001；Planta.2015,242:709-19)。基于此，从植物BAHD家族进化枝3和5中选择了二十一种候选物，这些候选物包括已鉴定出的参与多环二萜紫杉醇(taxol)生物合成的乙酰基转移酶(Proc Natl Acad Sci U S A.2000,18；97(2):583–587)和参与双环赖百当烷二萜毛喉素(Forskolin)的生物合成的来自毛喉鞘蕊花(Plectranthus barbatus)的两种乙酰基转移酶(Elife 2017,14；6:e23001)。

与候选植物相似，根据其公认的或实验确定的接受乙酰辅酶A作为酰基供体的能力及其底物的结构特征，选择了七种真菌乙酰基转移酶：带有空间位阻醇基团的大体积多环化合物(FEMS Microbiol Lett.2005,251:193-201；Chembiochem.2009,10:2325-8；Biotechnol Biotechnol Equip.2014,28(5):818-826；Nat Chem.2010,2:858-64)。其中，选择了参与芳基酸酯化的补身烷型倍半萜内酯astellolide(Sci Rep.2016,6:32865)的生物合成的蛋白AstG(来自米曲霉(Aspergillus oryzae)，NCBI登录号XP_023091083.1)。

另外，使用PSI Blast搜索Trends Biochem Sci.2002,27:161-4.)(标准参数和二次迭代)，基于其与AstG的序列相似性，从NCBI蛋白数据库中检索出二十一种推定的真菌乙酰基转移酶。使用NCBI Blast Tree View功能在系统树上可视化显示结果，以显示AstG与500PSI-Blast衍生序列之间的同源性。通过与查询序列的同源性选择候选物，这些查询序列来自系统树的单个分支。

最后，氯霉素乙酰基转移酶是混杂酶，能够容纳比氯霉素更大体积的底物(Protein Sci.2012,21(4):520–530)。因此，从NCBI蛋白质数据库中选出五种1至3类细菌氯霉素乙酰基转移酶作为假定的氯霉素乙酰基转移酶或从文献(Biochem J.1990,272:505-10)中选择。

表1：为将补身烷倍半萜转化为补身烷基乙酰化倍半萜而选择的乙酰基转移酶

筛选每一种酶候选物，以进行体内折叶苔醇向乙酸折叶苔基酯的生物转化。为了进行筛选，在工程化的酿酒酵母菌株中将乙酰基转移酶候选物与来自污叉丝孔菌(Dichomitus squalens)的折叶苔醇合酶XP_007369631.1，NCBI登录号XP_007369631.1的编码基因共表达，在该菌株中，具有提高水平的内源性法呢基二磷酸(FPP)。

为了增加酿酒酵母细胞中内源性FPP库的水平，涉及甲羟戊酸途径的所有酵母内源基因的额外副本，从编码乙酰辅酶A C-乙酰基转移酶的ERG10到编码FPP合酶的ERG20，与Paddon et al.,Nature,2013,496:528-532中所述的相似，在半乳糖诱导型启动子的控制下，整合到酿酒酵母菌株CEN.PK2-1C(Euroscarf，Frankfurt，Germany)的基因组中。简而言之，将三个盒分别整合在LEU2、TRP1和URA3基因座中。第一个盒包含在GAL10/GAL1双向启动子控制下的ERG20基因和一个截短的HMG1(tHMG1，如Proc Natl Acad Sci USA,1997,109:E111-8所述)，和同样在GAL10/GAL1启动子控制下的ERG19和ERG13基因，该盒的侧翼为两个100个核苷酸区域，分别对应于LEU2的上游和下游部分。第二个盒中，基因IDI1和tHMG1在GAL10/GAL1启动子的控制下，而基因ERG13在GAL7启动子区域的控制下，该盒的侧翼为两个100个核苷酸区域，分别对应于TRP1的上游和下游部分。第三个盒具有ERG10、ERG12、tHMG1和ERG8基因，均在GAL10/GAL1启动子的控制下，该盒的侧翼为两个100个核苷酸的区域，分别对应于URA3的上游和下游部分。三个盒中的所有基因都包含其自身终止子区域的200个核苷酸。而且，在ERG9启动子区域上游，整合了如Proc Natl Acad Sci USA,1991,88:8597-8601中所描述的，在其自身启动子的突变形式的控制下的GAL4的额外拷贝。另外，通过启动子交换修饰了ERG9的表达。使用含有带有其自身的启动子和终止子的HIS3基因的盒删除GAL7、GAL10和GAL1基因。将所得菌株与菌株CEN.PK2-1D(Euroscarf，Frankfurt，Germany)交配，获得称为YST045的二倍体菌株，其根据Solis-Escalante et al.,FEMS Yeast Res,2015,15:2诱导芽孢形成。孢子分离是通过将asci重悬于200μL、0.5M山梨糖醇和2μL的zymolyase(1000U mL

为了在YST069中表达XP_007369631.1和评估的乙酰基转移酶，如先前在Kuijperset al.,Microb Cell Fact.,2013,12:47中所述，使用酵母内源同源重组在体内构建质粒。质粒由用于酿酒酵母共转化的四个DNA片段组成。这些片段是：

a)通过用BsmBI进行酶促限制而线性化的质粒pF167(SEQ ID NO:1)。pF167以前是通过体内组装在酵母中构建的，它包含带有自身启动子和终止子的酵母标记LEU2，大肠杆菌标记AmpR，2μ酵母复制起点，大肠杆菌pUC复制起点和序列5'-GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG-3'(SEQ ID NO:2)和5'-AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT-3'(SEQ ID NO:3)用于同源重组；

b)一片段，其由序列5'-GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG-3'(SEQ ID NO:2)，酵母基因PGK1的终止子区域和倍半萜合酶XP_007369631.1DNA密码子(针对其在酿酒酵母中的表达进行了优化)(SEQ ID NO:4)组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)。通过PCR重叠延伸将来自酵母的双向GAL1/GAL10启动子区域添加到该片段中(Yolov and Shabarova.,Nucleic AcidsRes.1990,18(13):3983-6)；

c)一片段，其由与酵母GAL10启动子区域的头部核苷酸相对应的60bp，待评估的乙酰基转移酶DNA编码序列之一(针对其在酿酒酵母中的表达进行了密码子优化)和60bp的酵母CYC1终止子区域组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA94025)；和

d)一片段，由酵母基因CYC1的终止子区域和序列5’-AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT-3’(SEQ ID NO:3)组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)。

用体内质粒组装所需的片段来转化YST069。按照Gietz and Woods,MethodsEnzymol.,2002,350:87-96中所述，用乙酸锂方案进行酵母转化。将转化混合物铺板在含有6.7g/L无氨基酸的酵母氮碱(BD Difco，New Jersey，USA)，1.6g/L不含亮氨酸的滴注补充物(Sigma Aldrich，Missouri，USA)，20g/L葡萄糖和20g/L琼脂的SmLeu培养基上。将板在30℃下温育3至4天。使用单个菌落，在含有如Westfall et al.,Proc Natl Acad Sci USA,2012,109:E111-118中所述的250μL培养基和50μL己二酸二异癸酯(abcr GmbH,Germany)作为有机叠加层的深孔板中产生折叶苔醇和乙酸折叶苔基酯。将深孔板在平板培养箱中在30℃下温育3天。为了提取酵母细胞产生的折叶苔醇和乙酸折叶苔基酯，用700μL含内标物的乙酸乙酯提取深孔板的每个孔。使用GC-MS分析鉴定折叶苔醇和乙酸折叶苔基酯的产生，并使用上述内标通过GC-FID进行定量。

在这些实验条件下，对于9种折叶苔醇合酶和乙酰基转移酶的组合都检出了乙酸折叶苔基酯(表2)。值得注意的是，在非优化筛选条件下，两种活性最高的乙酰基转移酶(CrDAT和FgaAT)产生的乙酸折叶苔基酯滴度超过150mg/L。GC-FID色谱图显示在图2中。此外，图2显示，酵母衍生的乙酸折叶苔基酯的MS谱图与参考乙酸折叶苔基酯的MS谱图相同。

令人感兴趣的是，我们的筛选中活性最高的乙酰基转移酶CrDAT是植物BAHD家族进化枝3的成员，它参与植物生物碱的生物合成，但是，来自同一家族和进化枝的其他测试的乙酰基转移酶PsSalAT和RsVISY，其同样参与植物生物碱的生物合成(分别为蒂巴因(Thebaine)和维诺任碱(Vinorine))，当以折叶苔醇为底物时，并没有活性。另外，来自烟曲霉(Aspergillus fumigatus)的FgaAT同样参与生物碱的生物合成(对烟曲霉文(Fumigaclavine)B具有活性)，在我们的筛选中产生了大量的乙酸折叶苔基酯。相反，令人惊讶的是，推定的烟曲霉文B O-乙酰基转移酶GAO81666.1比FgaAT产生的乙酸折叶苔基酯少一个数量级。

值得注意的是，尽管AstG参与了经改性的补身烷型倍半萜Astellolide的生物合成，但乙酰基转移酶AstG或基于其与AstG相似性而选择的任何蛋白质(不包括GAO81666.1，参见上文)均未产生补身烷基乙酰化倍半萜乙酸折叶苔基酯。

在二萜生物合成中唯一鉴定出的乙酰基转移酶是那些参与紫杉醇(Taxol)和毛喉素产生的酶。从毛喉鞘蕊花(C.forskohlii)测试的八种乙酰基转移酶中，只有CfACT1-6和CfACT1-8能够乙酰化折叶苔醇。尽管基因TcTAT和TcDBAT能够在紫杉醇的生物合成中分别乙酰化结构相似的中间体(分别为乙酸紫衫-4(20),11(12)-二烯-5a-基酯和浆果赤霉素(Baccatin)III)，但只有TcTAT产生乙酸折叶苔基酯。

为了增加现已证明的复杂性，以鉴定能够使用折叶苔醇作为酰基受体的乙酰基转移酶，五种受试底物通用氯霉素乙酰基转移酶中只有一种能够将折叶苔醇转化为乙酸折叶苔基酯。

表2：发现能够在酿酒酵母中将折叶苔醇转化为乙酸折叶苔酯的乙酰基转移酶

为了在酿酒酵母中产生乙酸补身烯基酯，可以将如实施例2中所示能够将折叶苔醇转化为乙酸折叶苔酯的九种乙酰基转移酶(CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6，CfACT1-8)用于评估补身醇向乙酸补身烯基酯的转化。

通过共表达每种选择的乙酰基转移酶候选物与编码来自双孢蘑菇(Agaricusbisporus)的补身醇合酶XP_006461126(NCBI登录号XP_006461126)的基因，在工程化的酿酒酵母菌株YST069中体内产生乙酸补身烯基酯，在该菌株中，具有提高水平的内源性FPP(参见实施例2)。

为了在YST069中表达XP_006461126和所选的乙酰基转移酶(CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6和CfACT1-8)，如先前在Kuijpers et al.,Microb Cell Fact.,2013,12:47中所述，使用酵母内源同源重组在体内构建质粒。质粒由用于酿酒酵母共转化的四个DNA片段组成。这些片段是：

a)通过用BsmBI进行酶促限制而线性化的质粒pF167(SEQ ID NO:1)。pF167以前是通过体内组装在酵母中构建的，它包含带有自身启动子和终止子的酵母标记LEU2，大肠杆菌标记AmpR，2μ酵母复制起点，大肠杆菌pUC复制起点和序列5'-GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG-3'(SEQ ID NO:2)和5'-AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT-3'(SEQ ID NO:3)用于同源重组；

b)一片段，其由序列5'-GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG-3'(SEQ ID NO:2)，酵母基因PGK1的终止子区域和倍半萜合酶XP_006461126DNA密码子(针对其在酿酒酵母中的表达进行了优化)(SEQ ID NO:6)组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)。通过PCR重叠延伸将来自酵母的双向GAL1/GAL10启动子区域添加到该片段中(Yolov and Shabarova.,Nucleic AcidsRes.1990,18(13):3983-6)；

c)一片段，其由与酵母GAL10启动子区域的头部核苷酸相对应的60bp，待评估的乙酰基转移酶DNA编码序列之一(针对其在酿酒酵母中的表达进行了密码子优化)和60bp的酵母CYC1终止子区域组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)；和

d)一片段，由酵母基因CYC1的终止子区域和序列5’-AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT-3’(SEQ ID NO:3)组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)。

如用于产生折叶苔醇和乙酸折叶苔基酯的实施例2中所述进行酵母转化、筛选条件和补身醇和乙酸补身烯基酯的定量。

乙酸折叶苔基酯(来自实施例2)和乙酸补身烯基酯的相对量分别显示在图3和图4中。从测试的九种候选酶中，七种(CrDAT，FgaAT，OAH94415.1，TcTAT，GAO81666.1，CfACT1-6和CfACT1-8)产生了乙酸补身烯基酯。由于折叶苔醇和补身醇之间的结构相似性很高，因此预计会发现相似的相对转化率。

出乎意料的是，发现补身醇向其相应乙酸酯的转化发生了显著变化。特别地，发现产生最高相对量的乙酸折叶苔基酯的CrDAT是乙酸补身烯基酯的最低产生者之一。相比之下，发现FgaAT和CfACT1-8产生的乙酸酯相对量最高，而它们对折叶苔醇的活性较弱。这些令人惊讶的发现表明难以鉴定合适的乙酰基转移酶候选物以接受非生理底物。

a)实验1

为了鉴定能够乙酰化补身烷型倍半萜醇的其他乙酰基转移酶，在NCBI ProteinBlast搜索中使用了来自乙酰基转移酶CrDAT和FgaAT的氨基酸序列(分别在实施例2和3中显示)，它们对折叶苔醇和补身醇有活性，寻找接近的蛋白质序列同源物。使用默认参数执行Protein Blast搜索(Tatiana et al,FEMS Microbiol Lett.,1999,174:247-250,1999)。

从Blast搜索中检索到了与CrDAT同源的植物乙酰基转移酶XP_008340165.2和与FgaAT对应的4种真菌乙酰基转移酶(KEY80391，PYI04555.1，XP_001276734.1，XP_024709055.1)。它们在表3中列出。

表3：基于与CrDAT或FgaAT的氨基酸序列同源性而选择的用于将补身烷倍半萜转化为补身烷基乙酰化倍半萜的乙酰基转移酶

b)实验2

此外，还从地钱类(liverwort)鞭苔(Bazzania trilobata)和蕨类香鳞毛蕨(Dryopteris fragrans)的转录组中检索了额外的乙酰基转移酶候选物。地钱类鞭苔是富含萜类化合物包括补身烷倍半萜的来源。此外，乙酸折叶苔基酯和咖啡酸折叶苔基酯据报道是来自鞭苔属地钱的天然产物(Asakawa et al,Phytochemistry,Volume 30,Issue 9,1991,Pages 3037-3040)。类似地，在鳞毛蕨属中报道了几种不同的天然产物，包括乙酸折叶苔基酯。(Hideyuki Ito et al.Chem.Pharm.Bull.48(8)1190-1195(2000)；Froissard Det al.Nat Prod Commun.2014Jan；9(1):137-40.)

使用CLC基因组工作台(Qiagen)组装了鞭苔(Bazzania trilobata)(NCBI登录号ER364415)转录组，得到总共22083个重叠群(contigs)，平均长度为1,225个碱基对。CrDAT氨基酸序列用于在鞭苔(Bazzania trilobata)转录组中搜索同源序列。对于该搜索，以默认参数使用tBlastn算法(Altschul et al.1990,J.Mol.Biol.215,403-410)。E值超过0.001的转录物被考虑在内。选择属于植物乙酰基转移酶BAHD家族的10种转录物，其与CrDAT的氨基酸序列同源性低至20％。

香鳞毛蕨(D.fragrans)的植物材料是从中国北方收集的。将香鳞毛蕨的新鲜叶子(样品ID PNLI20141074)用于转录组分析。使用来自QIAGEN的RNeasy Plant Mini Kit(目录号74904)提取香鳞毛蕨的总RNA。使用用于Illumina的

进行了乙酰基转移酶的扩展筛选，以将折叶苔醇、补身醇和双环法呢醇转化为其相应的乙酸酯衍生物。在工程化的酿酒酵母细胞中体内筛选了实施例1的表1和实施例4中描述的总共89种乙酰基转移酶。不重复如实施例2和实施例3中所述在初步数目的乙酰基转移酶上产生的筛选数据。因此，仅筛选了产生在实施例2和实施例3中未测试的折叶苔醇、补身醇或双环法呢醇的乙酰基转移酶与酿酒酵母细胞的新组合。

如上文实施例2和实施例3中所述，工程化的酿酒酵母菌株YST069用于筛选乙酸折叶苔基酯和乙酸补身烯基酯的产生。

通过共表达每种选择的乙酰基转移酶候选物与负责从Astellolide生物合成中产生双环法呢醇的酶AstC(SEQ ID NO:138)、AstI(SEQ ID NO:140)和AstK(SEQ ID NO:142)(Yasutomo Shinohara et.al.Sci Rep.2016,6:32865)，在改造的酿酒酵母YST069菌株中体内产生乙酸双环法呢基酯。

为了同时表达AstC和AstI，构建了表达盒，其含有密码子和双向GAL1/GAL10启动子，该密码子针对啤酒酵母版本的基因进行了优化，该基因编码AstC(SEQ ID NO:138)和AstI(SEQ ID NO:140)，将该表达盒整合到YST069的基因组中，产生称为YST216的新菌株。通过DNA合成(ATUM，Menlo Park，CA 94025)获得了AstC和AstI的密码子优化的DNA序列。通过PCR重叠延伸将来自酵母的双向GAL1/GAL10启动子区添加到这些基因中(Yolov andShabarova.,Nucleic Acids Res.1990,18(13):3983-6)。

为了在YST216中表达AstK和评估的乙酰基转移酶，如先前在Kuijpers et al.,Microb Cell Fact.,2013,12:47中所述，使用酵母内源同源重组在体内构建质粒。质粒由用于酿酒酵母共转化的四个DNA片段组成。这些片段是：

a)通过用BsmBI进行酶促限制而线性化的质粒pF167(SEQ ID NO:1)。

b)一片段，其由序列5'-GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG-3'(SEQ ID NO:2)，酵母基因PGK1的终止子区域和AstK DNA序列密码子(针对其在酿酒酵母中的表达进行了优化)(SEQ ID NO:141)组成，该片段通过DNA合成获得(ATUM，Menlo Park，CA 94025)。通过PCR重叠延伸将来自酵母的双向GAL1/GAL10启动子区域添加到该片段中(Yolov and Shabarova.,Nucleic Acids Res.1990,18(13):3983-6)；

c)一片段，其由与酵母GAL10启动子区域的头部核苷酸相对应的60bp，待评估的乙酰基转移酶DNA编码序列之一(针对其在酿酒酵母中的表达进行了密码子优化)和60bp的酵母CYC1终止子区域组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)；和

d)一片段，由酵母基因CYC1的终止子区域序列5’-AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT-3’(SEQ ID NO:3)组成，该片段是通过DNA合成获得的(ATUM，Menlo Park，CA 94025)。

用体内质粒组装所需的片段转化YST216。如实施例2中所述进行酵母转化。双环法呢醇和乙酸双环法呢基酯的筛选条件和定量与实施例2中所述相同，不同之处在于使用25μL矿物油(2705-01,VWR International,LLC.)作为筛选过程中的有机覆盖物。

除了先前描述的对折叶苔醇具有活性的九种酶(CrDAT，FgaAT，OAH94415.1，TcTAT，CrMAT，LiAAT-4，GAO81666.1，CfACT1-6，CfACT1-8)(参见实施例2)，在这些实验条件下，当使用来自鞭苔(Bazzania trilobata)(SEQ ID NO:124或144)的乙酰基转移酶ERR364415-1_contig_8546和来自香鳞毛蕨(Dryopteris fragrans)(SEQ ID NO:118)的DfATC13时，还检测到乙酸折叶苔基酯。但是，它们的活性低于CrDAT和FgaAT。乙酸折叶苔基酯的相对量示于图5。

除了可以将补身醇转化为乙酸补身烯基酯的七种候选酶(CrDAT，FgaAT，OAH94415.1，TcTAT，GAO81666.1，CfACT1-6和CfACT1-8)之外，还发现有三种额外的乙酰基转移酶，来自费希尔曲霉(Aspergillus fischeri)的XP_001258079.1(SEQ ID NO:127)，来自鞭苔(Bazzania trilobata)的ERR364415-1_contig_8546(SEQ ID NO:124或144)，以及来自香鳞毛蕨(Dryopteris fragrans)的DfATC13(SEQ ID NO:118)，在扩展筛选中由补身醇产生乙酸补身烯基酯。所获得的乙酸补身烯基酯的相对量示于图6。图7显示，酵母来源的乙酸补身烯基酯的MS谱图与参考乙酸补身烯基酯的MS谱图相同。令人感兴趣的是，在这些实验条件下，ERR364415-1_contig_8546和DfATC13在所有测试的乙酰基转移酶中显示出最高的活性。

AstC、AstI和AstK与测试的89种乙酰基转移酶的13种组合检测到乙酸双环法呢基酯。图8显示，酵母衍生的乙酸双环法呢基酯的MS谱图与参考乙酸双环法呢基酯的MS谱图相同。乙酸双环法呢基酯的相对量示于图9。来自烟曲霉(Aspergillus fumigatus)的乙酰基转移酶FgaAT和来自鞭苔(Bazzania trilobata)的ERR364415-1_contig_8546显示了双环法呢醇的最高乙酰化活性。在这13种发现的乙酰基转移酶中，即如上文实施例2中所述的CrDAT，FgaAT，TcTAT，CrMAT，GAO81666.1，CfACT1-6，CfACT1-8，以及BAU61551.1(SEQ IDNO:133)，PsSalAT(SEQ ID NO:136)，XP_001217250.1(SEQ ID NO:130)，ERR364415-1_contig_8546(SEQ ID NO:124或144)，PYI04555.1(SEQ ID NO:121或143)，DfACT13(SEQ IDNO:118)(它们对双环法呢醇有活性)，也发现9种化合物，即CrDAT，FgaAT，TcTAT，CrMAT，GAO81666.1，CfACT1-6，CfACT1-8，ERR364415-1_contig_8546，DfACT13也对折叶苔醇和补身醇有活性。值得注意的是，参与芳酸酯化的补身烷型倍半萜内酯、astellolide(SciRep.2016,6:32865)生物合成的乙酰基转移酶AstG对测试的任何补身烷型倍半萜醇均无活性。这再次表明鉴定能够使用折叶苔醇、补身醇或双环法呢醇作为酰基受体的乙酰基转移酶的不确定性和复杂性。

交叉引用的文档内容通过引用并入于此。

本文所指的序列是：

NA＝核酸 AA＝氨基酸。

序列表

SEQ ID NO:1

质粒pF167

TGGTCAGCAACAACGCCGAAGAATCACTCTCGTGTTGAGAATTGCACGCCTTGACCACGACACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTTTGGCATTGGCAAAGTGCGGACTGCATAGTCACTGTGGTGCCGTACTTAGGGTACGCGTTCCTGAACGAAGCATCTGTGCTTCATTTTGTAGAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAAAGCGCTATTTTACCAACGAAGAATCTGTGCTTCATTTTTGTAAAACAAAAATGCAACGCGAGAGCGCTAATTTTTCAAACAAAGAATCTGAGCTGCATTTTTACAGAACAGAAATGCAACGCGAGAGCGCTATTTTACCAACAAAGAATCTATACTTCTTTTTTGTTCTACAAAAATGCATCCCGAGAGCGCTATTTTTCTAACAAAGCATCTTAGATTACTTTTTTTCTCCTTTGTGCGCTCTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTTAGAAGAAGGCTACTTTGGTGTCTATTTTCTCTTCCATAAAAAAAGCCTGACTCCACTTCCCGCGTTTACTGATTACTAGCGAAGCTGCGGGTGCATTTTTTCAAGATAAAGGCATCCCCGATTATATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGCGTTGATGATTCTTCATTGGTCAGAAAATTATGAACGGTTTCTTCTATTTTGTCTCTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTCACTCTATGAATAGTTCTTACTACAATTTTTTTGTCTAAAGAGTAATACTAGAGATAAACATAAAAAATGTAGAGGTCGAGTTTAGATGCAAGTTCAAGGAGCGAAAGGTGGATGGGTAGGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCAATATTTTAGTAGCTCGTTACAGTCCGGTGCGTTTTTGGTTTTTTGAAAGTGCGTCTTCAGAGCGCTTTTGGTTTTCAAAAGCGCTCTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTGCGCACATACAGCTCACTGTTCACGTCGCACCTATATCTGCGTGTTGCCTGTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATGCGTACTTATATGCGTCTATTTATGTAGGATGAAAGGTAGTCTAGTACCTCCTGTGATATTATCCCATTCCATGCGGGGTATCGTATGCTTCCTTCAGCACTACCCTTTAGCTGTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCTATCATTATGTCACCCGCAGTTCTGTGTCGTAGTCATCAACATAGCACCTATCCTTTGGCATCTCGGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAGTGAGACGTTAATTAAAGTAGACCGCTCACACATGGGCGGCCGCCGTCTCAAGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCATTCGACTACGTCGTAAGGCCGTTTCTGACAGAGTAAAATTCTTGAGGGAACTTTCACCATTATGGGAAATGCTTCAAGAAGGTATTGACTTAAACTCCATCAAATGGTCAGGTCATTGAGTGTTTTTTATTTGTTGTATTTTTTTTTTTTTAGAGAAAATCCTCCAATATCAAATTAGGAATCGTAGTTTCATGATTTTCTGTTACACCTAACTTTTTGTGTGGTGCCCTCCTCCTTGTCAATATTAATGTTAAAGTGCAATTCTTTTTCCTTATCACGTTGAGCCATTAGTATCAATTTGCTTACCTGTATTCCTTTACTATCCTCCTTTTTCTCCTTCTTGATAAATGTATGTAGATTGCGTATATAGTTTCGTCTACCCTATGAACATATTCCATTTTGTAATTTCGTGTCGTTTCTATTATGAATTTCATTTATAAAGTTTATGTACAAATATCATAAAAAAAGAGAATCTTTTTAAGCAAGGATTTTCTTAACTTCTTCGGCGACAGCATCACCGACTTCGGTGGTACTGTTGGAACCACCTAAATCACCAGTTCTGATACCTGCATCCAAAACCTTTTTAACTGCATCTTCAATGGCCTTACCTTCTTCAGGCAAGTTCAATGACAATTTCAACATCATTGCAGCAGACAAGATAGTGGCGATAGGGTCAACCTTATTCTTTGGCAAATCTGGAGCAGAACCGTGGCATGGTTCGTACAAACCAAATGCGGTGTTCTTGTCTGGCAAAGAGGCCAAGGACGCAGATGGCAACAAACCCAAGGAACCTGGGATAACGGAGGCTTCATCGGAGATGATATCACCAAACATGTTGCTGGTGATTATAATACCATTTAGGTGGGTTGGGTTCTTAACTAGGATCATGGCGGCAGAATCAATCAATTGATGTTGAACCTTCAATGTAGGGAATTCGTTCTTGATGGTTTCCTCCACAGTTTTTCTCCATAATCTTGAAGAGGCCAAAAGATTAGCTTTATCCAAGGACCAAATAGGCAATGGTGGCTCATGTTGTAGGGCCATGAAAGCGGCCATTCTTGTGATTCTTTGCACTTCTGGAACGGTGTATTGTTCACTATCCCAAGCGACACCATCACCATCGTCTTCCTTTCTCTTACCAAAGTAAATACCTCCCACTAATTCTCTGACAACAACGAAGTCAGTACCTTTAGCAAATTGTGGCTTGATTGGAGATAAGTCTAAAAGAGAGTCGGATGCAAAGTTACATGGTCTTAAGTTGGCGTACAATTGAAGTTCTTTACGGATTTTTAGTAAACCTTGTTCAGGTCTAACACTACCGGTACCCCATTTAGGACCAGCCACAGCACCTAACAAAACGGCATCAACCTTCTTGGAGGCTTCCAGCGCCTCATCTGGAAGTGGGACACCTGTAGCATCGATAGCAGCACCACCAATTAAATGATTTTCGAAATCGAACTTGACATTGGAACGAACATCAGAAATAGCTTTAAGAACCTTAATGGCTTCGGCTGTGATTTCTTGACCAACGTGGTCACCTGGCAAAACGACGATCTTCTTAGGGGCAGACATAGGGGCAGACATTAGAATGGTATATCCTTGAAATATATATATATATTGCTGAAATGTAAAAGGTAAGAAAAGTTAGAAAGTAAGACGATTGCTAACCACCTATTGGAAAAAACAATAGGTCCTTAAATAATATTGTCAACTTCAAGTATTGTGATGCAAGCATTTAGTCATGAACGCTTCTCTATTCTATATGAAAAGCCGGTTCCGGCCTCTCACCTTTCCTTTTTCTCCCAATTTTTCAGTTGAAAAAGGTATATGCGTCAGGCGACCTCTGAAATTAACAAAAAATTTCCAGTCATCGAATTTGATTCTGTGCGATAGCGCCCCTGTGTGTTCTCGTTATGTTGAGGAAAAAAATAATGGTTGCTAAGAGATTCGAACTCTTGCATCTTACGATACCTGAGTATTCCCACAGTTAACTGCGGTCAAGATATTTCTTGAATCAGGCGCCTTAGACCGCTCGGCCAAACAACCAATTACTTGTTGAGAAATAGAGTATAATTATCCTATAAATATAACGTTTTTGAACACACATGAACAAGGAAGTACAGGACAATTGATTTTGAAGAGAATGTGGATTTTGATGTAATTGTTGGGATTCCATTTTTAATAAGGCAATAATATTAGGTATGTGGATATACTAGAAGTTCTCCTCGACCGTCGA

SEQ ID NO:2

用于同源重组的序列

GCACTTGCTACACTGTCAGGATAGCTTCCGTCACATGGTGGCGATCACCGTACATCTGAG

SEQ ID NO:3

用于同源重组的序列

AGGTGCAGTTCGCGTGCAATTATAACGTCGTGGCAACTGTTATCAGTCGTACCGCGCCAT

SEQ ID NO:4

XP_007369631.1污叉丝孔菌(Dichomitus squalens)折叶苔醇合酶的DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGCTTCTATCCACAGAAGATACACTACTTTGATCTTGGACTTGGGTGACGTTTTGTTCAGATGGTCTCCAAAGACTGAAACTGCTATCCCACCACAACAATTGAAGGACATCTTGTCTTCTGTTACTTGGTTCGAATACGAAAGAGGTAGATTGTCTCAAGAAGCTTGTTACGAAAGATGTGCTGAAGAATTCAAGATCGAAGCTTCTGTTATCGCTGAAGCTTTCAAGCAAGCTAGAGGTTCTTTGAGACCAAACGAAGAATTCATCGCTTTGATCAGAGACTTGAGAAGAGAAATGCACGGTGACTTGACTGTTTTGGCTTTGTCTAACATCTCTTTGCCAGACTACGAATACATCATGTCTTTGTCTTCTGACTGGACTACTGTTTTCGACAGAGTTTTCCCATCTGCTTTGGTTGGTGAAAGAAAGCCACACTTGGGTTGTTACAGAAAGGTTATCTCTGAAATGAACTTGGAACCACAAACTACTGTTTTCGTTGACGACAAGTTGGACAACGTTGCTTCTGCTAGATCTTTGGGTATGCACGGTATCGTTTTCGACAACCAAGCTAACGTTTTCAGACAATTGAGAAACATCTTCGGTGACCCAATCAGAAGAGGTCAAGAATACTTGAGAGGTCACGCTGGTAAGTTGGAATCTTCTACTGACAACGGTTTGATCTTCGAAGAAAACTTCACTCAATTGATCATCTACGAATTGACTCAAGACAGAACTTTGATCTCTTTGTCTGAATGTCCAAGAACTTGGAACTTCTTCAGAGGTGAACCATTGTTCTCTGAAACTTTCCCAGACGACGTTGACACTACTTCTGTTGCTTTGACTGTTTTGCAACCAGACAGAGCTTTGGTTAACTCTGTTTTGGACGAAATGTTGGAATACGTTGACGCTGACGGTATCATGCAAACTTACTTCGACAGATCTAGACCAAGAATGGACCCATTCGTTTGTGTTAACGTTTTGTCTTTGTTCTACGAAAACGGTAGAGGTCACGAATTGCCAAGAACTTTGGACTGGGTTTACGAAGTTTTGTTGCACAGAGCTTACCACGGTGGTTCTAGATACTACTTGTCTCCAGACTGTTTCTTGTTCTTCATGTCTAGATTGTTGAAGAGAGCTGACGACCCAGCTGTTCAAGCTAGATTGAGACCATTGTTCGTTGAAAGAGTTAACGAAAGAGTTGGTGCTGCTGGTGACTCTATGGACTTGGCTTTCAGAATCTTGGCTGCTGCTTCTGTTGGTGTTCAATGTCCAAGAGACTTGGAAAGATTGACTGCTGGTCAATGTGACGACGGTGGTTGGGACTTGTGTTGGTTCTACGTTTTCGGTTCTACTGGTGTTAAGGCTGGTAACAGAGGTTTGACTACTGCTTTGGCTGTTACTGCTATCCAAACTGCTATCGGTAGACCACCATCTCCATCTCCATCTGCTGCTTCTTCTTCTTTCAGACCATCTTCTCCATACAAGTTCTTGGGTATCTCTAGACCAGCTTCTCCAATCAGATTCGGTGACTTGTTGAGACCATGGAGAAAGATGTCTAGATCTAACTTGAAGTCTCAATAA

SEQ ID NO:5

XP_007369631.1污叉丝孔菌(Dichomitus squalens)折叶苔醇合酶的氨基酸序列

MASIHRRYTTLILDLGDVLFRWSPKTETAIPPQQLKDILSSVTWFEYERGRLSQEACYERCAEEFKIEASVIAEAFKQARGSLRPNEEFIALIRDLRREMHGDLTVLALSNISLPDYEYIMSLSSDWTTVFDRVFPSALVGERKPHLGCYRKVISEMNLEPQTTVFVDDKLDNVASARSLGMHGIVFDNQANVFRQLRNIFGDPIRRGQEYLRGHAGKLESSTDNGLIFEENFTQLIIYELTQDRTLISLSECPRTWNFFRGEPLFSETFPDDVDTTSVALTVLQPDRALVNSVLDEMLEYVDADGIMQTYFDRSRPRMDPFVCVNVLSLFYENGRGHELPRTLDWVYEVLLHRAYHGGSRYYLSPDCFLFFMSRLLKRADDPAVQARLRPLFVERVNERVGAAGDSMDLAFRILAAASVGVQCPRDLERLTAGQCDDGGWDLCWFYVFGSTGVKAGNRGLTTALAVTAIQTAIGRPPSPSPSAASSSFRPSSPYKFLGISRPASPIRFGDLLRPWRKMSRSNLKSQ

SEQ ID NO:6

XP_006461126双孢蘑菇(Agaricus bisporus)补身醇合酶DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGCTCCACCACAAAGACCATTCACTGCTATCGTTTTCGACATCGGTGACGTTTTGTTCCAATGGTCTGCTACTACTAAGACTTCTATCTCTCCAAAGACTTTGAGATCTATCTTGAACTGTCCAACTTGGTTCGACTACGAAAGAGGTAGATTGGCTGAAAACGCTTGTTACGCTGCTATCTCTCAAGAATTCAACGTTAACCCAGACGAAGTTAGAGACGCTTTCTCTCAAGCTAGAGACTCTTTGCAAGCTAACCACGACTTCATCTCTTTGATCAGAGAATTGAAGGCTCAAGCTAACGGTAGATTGAGAGTTTACGCTATGTCTAACATCTCTTTGCCAGACTGGGAAGTTTTGAGAATGAAGCCAGCTGACTGGGACATCTTCGACCACGTTTTCACTTCTGGTGCTGTTGGTGAAAGAAAGCCAAACTTGGCTTTCTACAGACACGTTATCGCTGCTACTGACTTGCAACCACACCAAACTATCTTCGTTGACGACAAGTTGGAAAACGTTTTGTCTGCTAGATCTTTGGGTTTCACTGGTATCGTTTTCGACGAACCATCTGAAGTTAAGAGAGCTTTGAGAAACTTGATCGGTGACCCAGTTCAAAGAGGTGGTGAATTCTTGGTTAGAAACGCTGGTAAGTTGGGTTCTATCACTAGAACTACTGCTAAGCACGAATCTATCCCATTGGACGAAAACTTCGCTCAATTGTTGATCTTGGAAATCACTGGTAACAGAGCTTTGGTTAACTTGGTTGAACACCCACAAACTTGGAACTTCTTCCAAGGTAAGGGTCAATTGACTACTGAAGAATTCCCATTCGACTTGGACACTACTTCTTTGGGTTTGACTATCTTGAAGAGATCTAGAGAAATCGCTGACTCTGTTATGGACGAAATGTTGGAATACGTTGACCCAGACGGTATCATCCAAACTTACTTCGACCACAGAAGACCAAGATTCGACCCAGTTGTTTGTGTTAACGCTTTGTCTTTGTTCTACGCTTACGGTAGAGGTGAACAATTGAGATCTACTTTGACTTGGGTTCACGAAGTTTTGTTGAACAGAGCTTACTTGGACGGTACTAGATACTACGAAACTGCTGAATGTTTCTTGTACTTCATGTCTAGATTGTTGGCTACTTCTGGTGACCCAGACTTGCACTCTTTGTTGAAGCCATTGTTGAAGGAAAGAGTTCAAGAAAGAATCGGTGCTGACGGTGACTCTTTGGCTTTGGCTATGAGAATCTTGGCTTGTGACTTCGTTGGTATCAGAGACGAAGTTGACTTGAGAACTTTGTTGACTTTGCAATGTGAAGACGGTGGTTGGGAAGTTGGTTGGATGTACAAGTACGGTTCTTCTGGTATCTCTATCGGTAACAGAGGTTTGGCTACTGCTTTGGCTATCAAGGCTGTTGACACTATGTTCCAACCACAAATCAGATTCTCTGAATCTCCAACTGACACTTTGGTTGAAAACGCTATCCACAAGAGAAGACCATCTTTCTCTGAAAAGTTCTTGGGTAAGAGACCAAGATCTGGTTCTTTCAGAAAGCCATTGCAATGGATCTTGCAAGGTTCTAAGTTGAGAAAGTCTGTTGAAATCGGTTCTTAA

SEQ ID NO:7

XP_006461126双孢蘑菇(Agaricus bisporus)补身醇合酶的蛋白质序列

MAPPQRPFTAIVFDIGDVLFQWSATTKTSISPKTLRSILNCPTWFDYERGRLAENACYAAISQEFNVNPDEVRDAFSQARDSLQANHDFISLIRELKAQANGRLRVYAMSNISLPDWEVLRMKPADWDIFDHVFTSGAVGERKPNLAFYRHVIAATDLQPHQTIFVDDKLENVLSARSLGFTGIVFDEPSEVKRALRNLIGDPVQRGGEFLVRNAGKLGSITRTTAKHESIPLDENFAQLLILEITGNRALVNLVEHPQTWNFFQGKGQLTTEEFPFDLDTTSLGLTILKRSREIADSVMDEMLEYVDPDGIIQTYFDHRRPRFDPVVCVNALSLFYAYGRGEQLRSTLTWVHEVLLNRAYLDGTRYYETAECFLYFMSRLLATSGDPDLHSLLKPLLKERVQERIGADGDSLALAMRILACDFVGIRDEVDLRTLLTLQCEDGGWEVGWMYKYGSSGISIGNRGLATALAIKAVDTMFQPQIRFSESPTDTLVENAIHKRRPSFSEKFLGKRPRSGSFRKPLQWILQGSKLRKSVEIGS

SEQ ID NO:8

CrDAT DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGAAAGTGGTAAAATATCTGTAGAAACGGAAACCCTATCGAAAACCCTTATCAAGCCGTCCTCGCCCACGCCACAGTCTCTTTCCCGTTACAACTTATCTTACAATGATCAGAACATTTATCAGACCTGCGTATCTGTAGGGTTCTTTTACGAAAATCCTGATGGCATCGAGATATCCACGATCCGTGAGCAGCTGCAGAACAGCCTGTCCAAAACTCTGGTCTCATACTATCCCTTCGCAGGCAAAGTAGTGAAGAACGACTACATACATTGTAACGACGATGGCATTGAGTTCGTCGAAGTCAGAATTAGATGCAGGATGAATGATATACTTAAATACGAACTACGTTCTTACGCTCGTGATTTAGTCCTGCCAAAGAGGGTTACCGTGGGCTCTGAAGATACCACCGCTATCGTGCAGCTGTCCCATTTCGACTGTGGCGGTTTGGCCGTGGCTTTTGGTATCTCCCACAAGGTGGCCGATGGTGGTACTATAGCCTCTTTCATGAAGGACTGGGCGGCCTCTGCTTGCTACTTGTCCTCAAGTCACCACGTTCCAACGCCTCTATTGGTCTCAGACAGTATTTTCCCAAGACAGGACAACATAATATGCGAACAATTTCCCACCTCAAAGAATTGCGTGGAGAAGACATTCATTTTCCCACCAGAAGCAATCGAAAAGTTAAAGAGTAAGGCCGTTGAATTCGGCATTGAGAAACCGACCAGAGTAGAGGTCCTGACTGCATTCTTATCTAGATGCGCCACCGTAGCAGGTAAGTCGGCAGCTAAGAACAACAATTGTGGTCAAAGCCTGCCCTTTCCGGTTCTACAGGCCATTAATTTGAGGCCGATTCTAGAATTGCCACAGAACTCTGTGGGTAATCTAGTTTCGATCTACTTCAGCAGGACAATTAAGGAAAACGACTACCTAAATGAAAAGGAGTACACTAAATTGGTGATAAACGAGTTGCGTAAAGAAAAGCAAAAGATCAAGAATTTGAGCAGAGAGAAGTTGACCTACGTCGCCCAGATGGAAGAGTTCGTTAAGTCACTTAAAGAGTTCGATATCTCCAACTTCCTAGATATCGATGCCTACTTAAGCGATAGTTGGTGCAGGTTTCCTTTCTACGACGTCGACTTCGGATGGGGAAAGCCTATCTGGGTCTGCCTATTCCAGCCTTACATCAAGAACTGCGTTGTTATGATGGACTATCCTTTCGGTGACGATTATGGTATCGAGGCTATTGTTTCTTTCGAGCAAGAGAAAATGTCCGCGTTCGAGAAGAACGAACAGTTACTGCAGTTTGTGTCTAACTAA

SEQ ID NO:9

CrDAT蛋白质序列

MESGKISVETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYENPDGIEISTIREQLQNSLSKTLVSYYPFAGKVVKNDYIHCNDDGIEFVEVRIRCRMNDILKYELRSYARDLVLPKRVTVGSEDTTAIVQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAASACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVEFGIEKPTRVEVLTAFLSRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPILELPQNSVGNLVSIYFSRTIKENDYLNEKEYTKLVINELRKEKQKIKNLSREKLTYVAQMEEFVKSLKEFDISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYPFGDDYGIEAIVSFEQEKMSAFEKNEQLLQFVSN

SEQ ID NO:10

TcTAT DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGAGAAGACGGATTTACACGTGAACCTTATCGAAAAGGTTATGGTCGGTCCAAGCCCACCTTTGCCGAAGACTACACTACAACTATCCTCAATCGATAACCTACCTGGTGTTAGAGGCTCGATCTTTAATGCCTTGTTAATATATAATGCTTCACCGTCTCCCACGATGATCTCCGCTGATCCAGCTAAACCTATCAGAGAAGCTTTGGCCAAAATCCTGGTTTACTACCCACCTTTCGCTGGCAGGCTACGTGAGACTGAGAATGGCGATCTAGAGGTAGAGTGCACGGGAGAGGGTGCGATGTTTCTTGAAGCGATGGCCGATAATGAATTAAGTGTCTTAGGGGATTTCGACGACAGTAACCCTAGCTTCCAGCAATTGTTGTTCTCCCTTCCACTGGACACTAACTTCAAGGATCTATCCCTGTTAGTCGTGCAGGTGACAAGGTTTACATGCGGCGGGTTCGTCGTTGGGGTTTCATTTCACCACGGTGTATGCGATGGGAGAGGAGCTGCACAATTTCTGAAGGGACTAGCCGAAATGGCAAGGGGAGAAGTTAAGTTGTCGCTGGAGCCGATCTGGAATCGTGAACTAGTTAAGCTGGACGATCCCAAGTATTTGCAATTCTTTCACTTCGAATTCTTGAGAGCACCATCAATCGTCGAAAAGATAGTTCAAACCTACTTCATCATCGATTTCGAAACTATTAACTACATAAAGCAGTCAGTAATGGAAGAATGTAAAGAATTCTGCAGCTCTTTTGAAGTTGCTTCTGCAATGACGTGGATAGCTAGGACCAGGGCCTTTCAAATACCCGAGAGCGAGTACGTGAAAATCCTATTTGGTATGGACATGAGGAACTCTTTTAACCCGCCTCTGCCGTCAGGTTATTATGGAAACTCAATTGGTACGGCTTGTGCAGTGGACAATGTTCAAGACTTGTTGAGTGGTTCACTACTGAGGGCAATAATGATAATCAAGAAATCAAAGGTATCACTTAACGACAACTTTAAGAGCCGTGCTGTCGTTAAGCCTTCAGAACTTGACGTAAACATGAACCATGAGAATGTGGTGGCATTTGCTGACTGGTCAAGGCTGGGGTTCGACGAAGTCGATTTTGGCTGGGGAAACGCGGTGTCTGTTTCCCCGGTACAGCAACAGTCGGCACTTGCAATGCAGAATTACTTCCTGTTCTTGAAGCCAAGCAAGAACAAGCCCGATGGAATTAAGATTCTAATGTTCCTACCATTGTCCAAGATGAAATCCTTCAAAATTGAAATGGAAGCGATGATGAAGAAGTACGTCGCCAAGGTATAA

SEQ ID NO:11

TcTAT蛋白质序列

MEKTDLHVNLIEKVMVGPSPPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMISADPAKPIREALAKILVYYPPFAGRLRETENGDLEVECTGEGAMFLEAMADNELSVLGDFDDSNPSFQQLLFSLPLDTNFKDLSLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMARGEVKLSLEPIWNRELVKLDDPKYLQFFHFEFLRAPSIVEKIVQTYFIIDFETINYIKQSVMEECKEFCSSFEVASAMTWIARTRAFQIPESEYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSLNDNFKSRAVVKPSELDVNMNHENVVAFADWSRLGFDEVDFGWGNAVSVSPVQQQSALAMQNYFLFLKPSKNKPDGIKILMFLPLSKMKSFKIEMEAMMKKYVAKV

SEQ ID NO:12

CrMAT DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGATTCTATTACTATGGTTGAAACCGAGACATTATCGAAGACATTGATTAAGCCATCTAGCCCAACCCCACAGAGTCTGTCACACTACAACCTGTCTTACAACGATCAGAATATATATCCAGAGTATATATTTGCAGGCTTCTTCTACAGCAACCCGGACGGACACGAGATCTCGACTATTAGAGAGCAGCTGCAGAACTCTCTTAGCAAGACATTAGTATCTTATTATCCATTCGCTGGTAAAGTGGTCAAGAACGATTACATTCACTGCAATGACGACGGAATAGAATTCGTAGACGTCAGAATCCACTGCAGGATGAACGATATTCTTAAGCCTGAATTGAGGTCTTACGCTTCCGAGCTTATTCGTCCGAATAGAAGTACAGTGGGGTCAGAGGACAGTACTGCGCTTGTCCAGTTATCTCACTTTGACTGTGGCGGGGTAGCTGTCGCATTCGGTATATCTCACAAAGTCGCAGACGCAGCGACTATTCTGTCGTTTATAAAGGATTGGGCCGCTTCTACGTGTGACTTGTCAAGTAGTCACGATGTGTCTACACCAGTACTAGTATCCGATTCCATATTCCCCCGTCAAGATAACATCATCTGTGGCCAGTTCCCTGCTTCACCGAACTGCGTGAGGAAGCGTTTCTTATTCAGCCCGGAAGCTATCGAAAGACTAAAATCGAAAGCCATTGAATTTGGGATCGAGAAGCCAACGAGGGTAGAGGTCCTGACAGCATTCTTGTGCCGTTGCGCTACCGTTGCAGGTAAATCTGCGGCCAAGAACAACAATTGTGGACAGTCACTGCCTTTCGCTGTTATTCAAGCAGTCAACCTGAGGCCCTTACTAGAACTGCCAAAGAATTCCGTCGGCAACCTTATATCAATCTACTTTTCTACAATCAAAGAAAACGACACGGTGAACATCGAACAGGAGTTCACAAAATTAGTGATCGGAGAGTTGAGGAAGGCTAAGGATAAGTTAAAGAACCTGTCGCAAGAGAAGCTGAATTACGTAGCTAGAATGCAAGATTTCGCGAATTGCCTGAAGGAATTGGACATAAGTTCATTCTTCGACATGGAAAACGTGGACATAGACGCTTATTTATTTTCGAGCTGGTGCAGGTTCCCCTTCTACGACATCGATTTCGGTCTGGGGAAGCCAATATGGGTCTGCATGTTTCAGCCTCACTTTAAGAATTGTATAATTTTAATGGATTATCCCTTTGGTGACGATTACGGCATCGAAGCCCTAATTACGTTGGAACAAGAGAAGATGCCCGCCTTCGAAAACAACGAGCTGCTGCTAAGCTTCGCCAGCAACTAA

SEQ ID NO:13

CrMAT蛋白质序列

MDSITMVETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPDGHEISTIREQLQNSLSKTLVSYYPFAGKVVKNDYIHCNDDGIEFVDVRIHCRMNDILKPELRSYASELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAASTCDLSSSHDVSTPVLVSDSIFPRQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAIEFGIEKPTRVEVLTAFLCRCATVAGKSAAKNNNCGQSLPFAVIQAVNLRPLLELPKNSVGNLISIYFSTIKENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVARMQDFANCLKELDISSFFDMENVDIDAYLFSSWCRFPFYDIDFGLGKPIWVCMFQPHFKNCIILMDYPFGDDYGIEALITLEQEKMPAFENNELLLSFASN

SEQ ID NO:14

LiAAT-4DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGCCATGATAATCACGAAGCAAATATTAAGACCTAGTTCCCCGACGCCCCAAGCCTTTAAGAATCACAAGCTGTCCTACTTAGACCAAATACAGGCGCCTATTTACATACCTTTGTTGTTCTTCTATAAGAACGAGGAGTCAAAATACCCAGACCAGATCTCGCAAAGATTTAAGCAGAGTTTGTCCGAAATTTTGACAATATTCTACCCTTTGGCTGGTACGATGAGGCATAACTCGTTCGTGGACTGCAATGACAGGGGTGTCGAATTTGTAGAGGTCAGGGTCCATGCGAGACTAGCCCAGTTCATTCAAGATCCTAAGATGGAAGAGCTTAAGCAATTGATACCTGTGGATTGTATATCTCACACTGACGATGATTTCTTATTGCTAGTCAAGATTAGCTATTTTGACTGCGGTGAGGTCGTCGTTGGAGTCTGCATGTCGCACAAAATTGGTGACGGAATTAGTCTGGCAGCGTTTATGAACGCTTGGGCAGCAACGTGTAGGGGAGAATCGTCTAGTGAGATCATTCACCCATCTTTTGATCTTGCTTTACACTTTCCGCCTAAAGACCACTTGTCTTCAGCATCCTCATTCCGTGTGGCCATAGCCCAGGAGAACATCATGACCAAGAGGCTAGTATTTGATAGAGAAAAGTTGGAGAAGCTGCGTAAGAGAATCGCTGCCAGTTCTGATGGGGTGAGAGACCCTAGCAGAGTTGAAGCTGTATCTGTCTTTATTTGGAAAAGCTTAATTGAAGCCCACAAGGCCGAGTCACACATGACTGAGACACCAGCCGTTTCTATTGCTAGCCACGCCGTGAACTTAAGGCCTAGAACAGTCCCACAAATGGACCAAACTTTCGGTAACTGCTACGCTCCCGCTTCGGCCGTTGTCTCCTGGGATGAAGACTACGTACATCACAGTCGTTTGAGGGCGGCCCTTAGAGAAATCGACGACGACTACATTAATAAGGTCTTGAAAGCCGACAATAATTATTTAACGCAGGATCAAATTGGTGACTTGTTTAAACCAGAAAACTCAGTTCTAAGTTCGTGGTGGCGTTTCCCAGTTTACAAGGTAGACTTTGGATGGGGTAAGCCAGTTTGGGTTTCTACGACGACCATCCAATACATGAACTTGATTATATTTACTTCGACGCCCTCAGAAGACGGCATAGAGGCGTGGGTAACTACTACTCACAATTTCTTCCAAGTTCTGCAGGCAAACTATAATAAACTTGACACGTAA

SEQ ID NO:15

LiAAT-4蛋白质序列

MAMIITKQILRPSSPTPQAFKNHKLSYLDQIQAPIYIPLLFFYKNEESKYPDQISQRFKQSLSEILTIFYPLAGTMRHNSFVDCNDRGVEFVEVRVHARLAQFIQDPKMEELKQLIPVDCISHTDDDFLLLVKISYFDCGEVVVGVCMSHKIGDGISLAAFMNAWAATCRGESSSEIIHPSFDLALHFPPKDHLSSASSFRVAIAQENIMTKRLVFDREKLEKLRKRIAASSDGVRDPSRVEAVSVFIWKSLIEAHKAESHMTETPAVSIASHAVNLRPRTVPQMDQTFGNCYAPASAVVSWDEDYVHHSRLRAALREIDDDYINKVLKADNNYLTQDQIGDLFKPENSVLSSWWRFPVYKVDFGWGKPVWVSTTTIQYMNLIIFTSTPSEDGIEAWVTTTHNFFQVLQANYNKLDT

SEQ ID NO:16

FgaAT DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGAAGAAGCAGGTCACTTTTAAACCTTTCAGATTGTCACCAGTCGATCATAGTTTACCTAAGGTTTACATCTTCAAGTCTCTATATTTCAGAGGGGTAGATGACACTGGTTCTCTTAGTAGACTACAGGATGGCATTGATCGTTTGATATCTTGTCTGCCTTTCCTGTCAGGCGAGGTGGTTCCATGTGCAGACATACCAGATAAGGTCGGTGTACTTCAAGTTCAGATGCCTTGCCCTTCCTTGCAAGAAATTCCAATGCTGCTAGTCAAAAGTTATCCAAACCATACATGGCCAGCAGCTTCTACCTCGGAAAGATGGAGAAACACCGCTTTGCTAGATCAGTCGTATAGGCCATTGCCGGATTTTATACCCCCATCAAAGCCTAGACCTGTTTTGAGATTTCAGGCGAACTTCCTGGCTGATGGTTTAATGCTATGTATGGGATATAATCATTCTGTATTCGACGGAACAGGTGCGGGGAACATTCTGGAAATGTTGGCTGATTGTTGTAGAGCTAATCCAAACTCCATCTTAGCACTGCCAACAAACGGTGACATAGAGAGCGAGTTGAGAGGTTTACTGTCCAGTCCAGGTGTAGCAGTTGCAAATGCCTCTCAGGAAGCCTACGCAATTAATTGTGCACACACGGAAGTAGAACCCGAACCCAGTTCAGCTATGCTTTACTGTTGGCCTTTCTTGCTGAGTTCTGAGAAGATTGAATGTTTACAGGAAGCATGTAATAGTTTACTACCACATATCGTTCGTTTGTACTCTGGTACGCAGAGTTCGCTTATAAACCAAGATACGAACTGGCCACATATTCTTTCATCAAACGACGTGCTAACCGCTCTTTTAGCTGTTTCAATTGAAAAGGCTAGGGAAGCAACAGGTGCCCTTGGTCATATGAGCAGATCATTGGCTATGGCTGTTAACCTACGTGAGCGTTTAAAGCCTATGCCAAGACACTATTTGGGTAACCTAGTTACCACTGTGTGGGTATCACATCACCGTCCTGCCGTTAAGGACCTTGAAACAATGGTTTTACCAGTGCCAGCATGCAATAGGCACGAAATAGACAGAGACGACTTGTTGTGGATAACCCACGTGGCATTCCGTATTAGATTAGGGCTGAACGCAATAAACGAAGAACATATTAGAGGTCTGATCCACTACTTGCATTCCCAAGACGATTGGGAACAGATTGGAATACATTTCACCGATCCAATTTTCATTTCTTCCTGGCGTCACCTTAAGGTCTATGAACTAGACTTCGGACCTACTATCGGTCATGCTGAACACTTTGAGATGGATGTCGGCACCACCGATGGTGTGTGTGTTGTTATGCCTGCTAACACCAGAGCCGTCGGCAAGACTAAGAAGGCTCCTTGGGACATTAGAATCGTGTTAAACCCCGAAGTGTTACAGGCACTAATAGCTAGTGCCATCTTTGGTTGGGCTATGGTCAAGGACGCTTCGACATAA

SEQ ID NO:17

FgaAT蛋白质序列

MKKQVTFKPFRLSPVDHSLPKVYIFKSLYFRGVDDTGSLSRLQDGIDRLISCLPFLSGEVVPCADIPDKVGVLQVQMPCPSLQEIPMLLVKSYPNHTWPAASTSERWRNTALLDQSYRPLPDFIPPSKPRPVLRFQANFLADGLMLCMGYNHSVFDGTGAGNILEMLADCCRANPNSILALPTNGDIESELRGLLSSPGVAVANASQEAYAINCAHTEVEPEPSSAMLYCWPFLLSSEKIECLQEACNSLLPHIVRLYSGTQSSLINQDTNWPHILSSNDVLTALLAVSIEKAREATGALGHMSRSLAMAVNLRERLKPMPRHYLGNLVTTVWVSHHRPAVKDLETMVLPVPACNRHEIDRDDLLWITHVAFRIRLGLNAINEEHIRGLIHYLHSQDDWEQIGIHFTDPIFISSWRHLKVYELDFGPTIGHAEHFEMDVGTTDGVCVVMPANTRAVGKTKKAPWDIRIVLNPEVLQALIASAIFGWAMVKDAST

SEQ ID NO:18

GAO81666.1 DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGAAGAACATACCAGATCCTTTGAGCCATTTGACCTGGCCTGCTTAGATCATACTGTGGGGCCGGTTTTCATGAATTTCTTCTTAAGTTTCAAGCCTGCCAAGATCGAAGAGAGTCTTATCTCAATTGAAGAAGGTGTAACTAGGTTAGTCAACAGACTGCCATTCCTGGCCGGTGACGTTGTTAATTCTGAAAACGTAGACGGTCGTGTTAATGTAATGAGGATTCAGCCAAGCTCGACATTGATTAGAGAGATTCCCATGCTACACACAAAGCACCATCCTCATCATATTTTGCCGAATACACCTAGACAGATTAACCCAGGGTCCGACCAGGATCAAAGGTTTGCTCCCTTGGACGATTCGTACGTACCACCAGTGTCTTTATTACCTCTTGCTCCGGGCCCAAGGCCCGTAGTAAGATTCCAAACTAATGTTGTAATTGACGGGATTGTGCTTGCACTTGGGTTCCACCACTCAGTATTTGATGCTACCGGAGTGGGTTTGTTGATCGAAATGTTGGCCACATGTTGTTCAAGCGATTGTCCTGCCCTATCATCCCATATTGAACTGGAAGAAGAAATAAGGCTAAGGCGTTCTGTGGACAAGATAGGTAACGGCGCTACTGATTTAGCATCCCAAAGAGATGAAGTTCAGGACCCAAATGGCAGTATCACTGAGTCCCCAGCTCATGTGCCTATGGGTGACAGTAGCTGGGTACCCCCTAAACTATCTGTTTACTCCTTTAATTTATCGGCTGCCGGTTTGGCTCATTTGAAGACAGCCTGTAACAAGTTATTGCCCGCTATCCATGCGAGTCAAAATGGTTCACCACAAAGTGCGGAATCAGAAGGTGAGAAATTACAGCAAGAATTCGTTTCGACGAATGATGTTCTAACGGCTTTGTTGGCTACTTCCATTCACCAAGCGAGGTCTAGAGTTACTGAAACGGAATTAGTTCCGACAAAAGCGAAACTTGCGATGGCCGTTAATTTGAGGGAAAGGGCCAGTTCGTTACCCAAGACTTATTTGGGGAACTCGCTAACAGTTACAGAAGCTTTTGTGTATTCTTTAGCTGCTACCGACGGTTTCGATGGTGTCCCCGAGAGACATTATCATCCTGACTTGCGTAACGCCTTGCTATTAGAAATTGCTAGAGTTGCATTGCAATTGAGAAAAGGGCTGGCAGCGATAGATGACGCGTATTTTAGGCAATTTGTGTCTAGATTGAGGGCTAATCTAGACTGGTCACAGTTAGGTGCAAACCTGCCTGACACTATGGTGTCAAGCTGGAGACATTTGAAAGTATATCGTTTAGACTTCGGTGCTAGATTGGGGAGAGTTGTAGAGTTCCATCCACAAACCGCTTTGGTTGACGGTATTTGTATCATCCAACCTGAAAGGATAGCCCATGAGGATGATTCAGCTGATATGGCTCCAGAATCTGGGTGGGAAGTTTGTGTAACATTACAAAGCGATGCCATGGAGTGCTTCTTAAGAGGTGGGTTGTTCACAAGTCTTTCTCAAGGTGCAATTAGAAGAGTTTAA

SEQ ID NO:19

GAO81666.1蛋白质序列

MEEHTRSFEPFDLACLDHTVGPVFMNFFLSFKPAKIEESLISIEEGVTRLVNRLPFLAGDVVNSENVDGRVNVMRIQPSSTLIREIPMLHTKHHPHHILPNTPRQINPGSDQDQRFAPLDDSYVPPVSLLPLAPGPRPVVRFQTNVVIDGIVLALGFHHSVFDATGVGLLIEMLATCCSSDCPALSSHIELEEEIRLRRSVDKIGNGATDLASQRDEVQDPNGSITESPAHVPMGDSSWVPPKLSVYSFNLSAAGLAHLKTACNKLLPAIHASQNGSPQSAESEGEKLQQEFVSTNDVLTALLATSIHQARSRVTETELVPTKAKLAMAVNLRERASSLPKTYLGNSLTVTEAFVYSLAATDGFDGVPERHYHPDLRNALLLEIARVALQLRKGLAAIDDAYFRQFVSRLRANLDWSQLGANLPDTMVSSWRHLKVYRLDFGARLGRVVEFHPQTALVDGICIIQPERIAHEDDSADMAPESGWEVCVTLQSDAMECFLRGGLFTSLSQGAIRRV

SEQ ID NO:20

CfACT1-6 DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGAAGGTAGAACGTTTCTCAAGAAAGTTGATAAAACCCCACACGCCTACTCCCGAGAACTTAAAGAAATATAAATTGTCTCTATTGGACAAATGCCTTGGACACGACAACTTCGCGATCGTGTTGTTTTACGAATCTAAACCAAGAAATAAGAGTGAGCTTGAGGAATCGTTAGAGAAGGTCTTGGTAGACTTTTATCCCTTGGCTGGGCGTCACACGATGAACGACCATATTGTCGACTGCTCGGATGTCGGCGCTGTCTTTGTAGAAGCCGAGGCTTTAGATGTAGAGTTGACTATGGATGAGTTAGTCAAGAACATGGAAGCTCAAACTATACACCACTTGTTGCCAAATCAGTACTTCAGTGCAGATGCTCCGAACCCGCTGCTTTCCATCCAAGTTACGCACTTTCCTTCCGGTGGTTTGGCTATCGGAATCGCAGTCTCGCACGCTGTATTTGATGGTTTCTCTTTGGGCGTGTTCGTAGCAGCATGGTCAAAGGCGACCATGAACCCGGATAGGAAAATCAAAATAACACCGTCATTCGACTTACCATCACTTCTGCCCTACAAAGACGACAACTTTGGTTTGACTGCTGCTGAAATTGTCAGCCAGAGCGAGGACATCGTAGTTAAGAGATTTATCTTCGGCAAGGAAGCCATCACGAGGTTGAGAAGTAAGCTTAGTCCAAATAGGAACGGGAAGAAAATATCCAGGGTTAGGGTCGTTTGTGCAGTCATTGTAAAGGCCTTGATGGGATTGGAGCGTGCCAAACACGGTAAGACGCGTGATTTCTTAATTACTCAATCAATTAACATGAGGGAGAGAACTAAGGCGCCGCTGCAGAAACACGCCTGCGGCAACTTAGCAGTCTTGAGTTGCACGAGAAGAGTAGAGGCCGAGGAGATGATGGAGTTACAGAACCTAGTTAATTTGATCGGCGACAGTACCGAAAAGGACATCGCCGACTTTGCAGAATTATTATCACCAGATCAAGTGGGCAGAGACATTATAATCAAGATGATGAAGTCATTCATGCAGTTCTTGGACAATGACATTTACAGCGTGTGCTTTACCGATTGGTCAAAGTTTGAATTTTACGAAGCGGACTTCGGCTTCGGGAAGCCGGTTTGGATGGCAGCAGGCCCACAGAGACCCATTATCTCTACTGCAATATTGATGTCAGACAGGGAAGGCGACGGTATCGAGGCTTGGTTACATTTGAATAAGAACGACATGTTGATTTTCGAACAAGACGAGGAAATCAAGTTATTCACTACATAA

SEQ ID NO:21

CfACT1-6蛋白质序列

MKVERFSRKLIKPHTPTPENLKKYKLSLLDKCLGHDNFAIVLFYESKPRNKSELEESLEKVLVDFYPLAGRHTMNDHIVDCSDVGAVFVEAEALDVELTMDELVKNMEAQTIHHLLPNQYFSADAPNPLLSIQVTHFPSGGLAIGIAVSHAVFDGFSLGVFVAAWSKATMNPDRKIKITPSFDLPSLLPYKDDNFGLTAAEIVSQSEDIVVKRFIFGKEAITRLRSKLSPNRNGKKISRVRVVCAVIVKALMGLERAKHGKTRDFLITQSINMRERTKAPLQKHACGNLAVLSCTRRVEAEEMMELQNLVNLIGDSTEKDIADFAELLSPDQVGRDIIIKMMKSFMQFLDNDIYSVCFTDWSKFEFYEADFGFGKPVWMAAGPQRPIISTAILMSDREGDGIEAWLHLNKNDMLIFEQDEEIKLFTT

SEQ ID NO:22

CfACT1-8 DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGAAAGTCGAAAGGATTTCACGTAAATTCATCAAGCCATATACACCTACACCACAGAACCTTAAGAAGTACAAGCTATCCTTGCTGGATAAATGCATGGGACACATGGACTTCGCTGTAGTATTGTTTTACGAATCAAAGCCAAGAAACAAGAATGAGCTGGAAGAATCACTAGAGAAAGTGTTAGTCGATTTCTATCCATTGGCAGGCAGGTATACCATGAACGACCACATTGTCGATTGCAGCGATGAGGGCGCCGTTTTCGTTGAGGCAGAGGCCCCTAATGTTGAGCTTACAGTGGACCAGTTGGTTAAGAACATGGAAGCCCAGACAATCCACGACTTCTTACCAGACCAATATTTTCCTGCTGACGCACCAAATCCGTTGCTAAGTATTCAAGTAACGCACTTCCCTTGTGGTGGTTTAGCTATCGGGATTGTTGTTAGTCACGCGGTCTTTGATGGATTCTCATTGGGCGTATTCTTAGCCGCCTGGAGCAAAGCTACCATGAACCCTGAGAGGAAGATCGAAATCACCCCTTCCTTCGATTTGCCTAGTCTTCTGCCCTACAAGGATGAATCTTTCGGTTTAAATTTTAGCGAAATTGTCAAAGCTGAGAATATCGTAGTTAAACGTTTGAATTTCGGGAAAGAGGCTATTACGCGTTTGAGGTCCAAGCTGTCTCCTAACCAGAATGGTAAAACCATTTCCAGAGTGAGGGTAGTCTGCGCGGTGATAGTTAAGGCGTTGATGGGACTGGAGAGAGCAAAGACTAGAGACTTTATGATATGTCAGGGGATCAACATGAGAGAGAGGACGAAGGCGCCCCTGCAGAAGCATGCGTGTGGCAACCTAGCAGTTTCGTCTTACACTAGAAGGGTAGCGGCAGCGGAAGCAGAAGAACTGCAGTCCTTAGTGAATTTGATCGGGGACTCTATCGAAAAGTCAATCGCAGACTACGCTGATATACTTTCGAGTGATCAAGATGGGAGACACATCATTTCCACTATGATGAAGAGCTTTATGCAGTTTGCTGCACCTGATATAAAAGCCATTTCATTTACCGACTGGTCAAAGTTCGGCTTTTACCAAGTAGATTTTGGTTTTGGTAAACCAGTTTGGACCGGCGTCCGTCCAGAACGTCCAATCTTCTCAGCCGCGATATTGATGAGTAACAGGGAAGGCGATGGCATCGAGGCTTGGCTTCATTTGGACAAGAACGATATGCTAATATTTGAGCAAGACGAAGAAATTAAGTTGTTGATAACGTAA

SEQ ID NO:23

CfACT1-8蛋白质序列

MKVERISRKFIKPYTPTPQNLKKYKLSLLDKCMGHMDFAVVLFYESKPRNKNELEESLEKVLVDFYPLAGRYTMNDHIVDCSDEGAVFVEAEAPNVELTVDQLVKNMEAQTIHDFLPDQYFPADAPNPLLSIQVTHFPCGGLAIGIVVSHAVFDGFSLGVFLAAWSKATMNPERKIEITPSFDLPSLLPYKDESFGLNFSEIVKAENIVVKRLNFGKEAITRLRSKLSPNQNGKTISRVRVVCAVIVKALMGLERAKTRDFMICQGINMRERTKAPLQKHACGNLAVSSYTRRVAAAEAEELQSLVNLIGDSIEKSIADYADILSSDQDGRHIISTMMKSFMQFAAPDIKAISFTDWSKFGFYQVDFGFGKPVWTGVRPERPIFSAAILMSNREGDGIEAWLHLDKNDMLIFEQDEEIKLLITT

可选地，C-末端的“T”可缺失

SEQ ID NO:24

OAH94415.1 DNA序列，针对其在酿酒酵母中的表达经密码子优化

ATGGATACCAAGAGGGTCGGTTATACAGTCGTTGATTTGAGTCAGTGGGGCAGAAAAGAACACTTCGAAGCATTCCAGAGCTTCGCCCAGTGCACCTTTTCCCAGACTGTTCAATTAGACATAACTTCCCTGCTGAAGACTGTCAAGCAGAACGGATATAAATTCTACCCGACCTTTATCTACATAATAAGCCGTTTAGTGAACAAGCATGCCGAATTCCGTATGGCCATGAAGGATGGTGAACTGGTAATTTGGGACTCCGTAAACCCTGGTTACACAATCTTTCACGAACAGACCGAAACATTTTCATCTCTGTGGAGCTATTACCACAAGGACATCAATCAGTTTCTTAAAACGTATTCTGAAGACATCGCTCAGTACGGGGACGATCTGGCGTACTTCCCCAAGGAGTTTATTGAAAATATGTTCTTCGTGTCAGCGAACCCATGGGTGAGCTTCACAAGTTTCAACTTAAACGTGGCCAATATTAATAACTTCTTCGCACCGGTCTTTACAATCGGTAAATACTACACGCAGGGCGACAAGGTGTTGATGCCGTTAGCTATTCAGGTCCATCATGCAGTCTGCGACGGGTTTCATGTCGGCAGGTTATTGAACGAAATTCAGCAGTACTGCGATGAGGGATGCAAGTAA

SEQ ID NO:25

OAH94415.1蛋白质序列

MDTKRVGYTVVDLSQWGRKEHFEAFQSFAQCTFSQTVQLDITSLLKTVKQNGYKFYPTFIYIISRLVNKHAEFRMAMKDGELVIWDSVNPGYTIFHEQTETFSSLWSYYHKDINQFLKTYSEDIAQYGDDLAYFPKEFIENMFFVSANPWVSFTSFNLNVANINNFFAPVFTIGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRLLNEIQQYCDEGCK

SEQ ID NO:116

DfACT13天然核苷酸序列

ATGGCCTGTGGAGGCGGCGTGAGGAATATTGAGGTGAAGGCGCAGGAGCCCGTTTTGGTGCAGCCTTTATCTACAGCACAAAGCTCTGCCTATAATCTTCTTACAAAACTGGACCAGACGCTCGCACAATTGGTGGTGCAGATTGTCTTTGTCTTCGATGTGAAAAACCCCGTAACCCGCCGACCCCACGATGGCGCCGATCCTGCCAAGCTTCTGAAGGAGGCTCTGAGGAAGGTCCTTGTGCCCTTCTACCCGCTCGCTGGGCGTCTTTGCCTCTCGCCCGATGACGGCAGTCTGTTCATTGACTGCAATGCTCAGGGGGTTTCCTTTGTTGAGGCCAATGCCGACGCGGATATCTCCGAACTCGGCGACTTCTCGCAGCCCGATTTCGCGACTCTGGGCTCCCTTGTCTTCCCCTTACCTCCTATAGCTTCGGATGATGGTCCTCTTCTATCTGCGCAGGTGACCAGATTCAAGTGTGGAGGATTTGTGCTGGGCTTCGTATTCCATCATTGTTTATTTGATGGATTCGCACTTTCGGAATTCCTAAATGCGTGGGCGGAGACTGCATGCGGCGTGCCTCTTTCTACACCTCCTGTCCTCGACAGAACTTTTCCGAGGGCGCGTTCTCCCTTGCAAATCAAGTATCCCCACACCGAGTTCCTGGAAGTCGAAGATGTCTCTTTGACTCAAAATATCTCTAACGATGCCATCAACCGGTCTTTCTGCTTTACTTCGGCAAGTCTAGAGATACTCAAGAAGAAAGCCTTGGAGGATGGGGTGCTATCAAAATGCACTACTTTTGAAGCTTTATCTGGGCTGATATGGAGGGCCCGAACTAGAGCCCTGTGGAGCGATTACCCGGAGCACAAACTAAAGGTGCTCATTGTCGTCGACCCGAGAGCACGTTTTGAGCCTCGTGTGGTGCCAAAAGGGTATGTGGGCAATGCGGTGCTTTTTACATGTGCTTTCGCAAGCGCAAGGGAGCTGGAAGAAAATCCCTTGTCACATGCAGTGAAGCATGTGCAACATGCCATCGGGCGTATGACGGAGGAGTACATGTTGTCGCAAATCGACTACATGGAGCATCAGAAGGTATGGTGCCCACCGCTAGGAGCTAGTACATCTTTCATGACCAAATGGTCTAGGTTGGCCTTCAATATTCTAGACTTTGGGTGGGGCAGGCCGAAGTATGTGGGGCCGGCCACGTCGCTGTCGATGGAGACGACTACTTTTGTATCTTATGGAAAGGGCATGAGTGTGGTGTTGGCTCTTCCTCCAGAAGCAATGCGCAAATTTGAAAAAATCGTACATCCCTACCTCAATCCATGA

SEQ ID NO:117

DfACT13核苷酸序列，针对其在酿酒酵母中的表达经密码子优化.

ATGGCATGTGGCGGTGGGGTCAGGAATATCGAAGTGAAGGCTCAAGAGCCAGTCTTGGTGCAACCGTTAAGCACTGCTCAATCGTCAGCCTACAATTTGTTAACAAAGTTAGACCAGACTTTGGCGCAATTGGTTGTCCAAATAGTGTTTGTTTTCGATGTGAAGAACCCTGTGACAAGAAGGCCTCATGATGGAGCCGATCCAGCTAAGTTATTGAAAGAGGCCTTAAGGAAAGTACTAGTACCATTCTATCCCCTTGCCGGAAGACTATGTCTTTCCCCAGACGATGGTAGTCTATTCATAGACTGCAACGCTCAAGGTGTTTCATTTGTCGAAGCAAACGCTGATGCCGACATCAGTGAGTTAGGTGATTTCTCCCAACCGGATTTTGCGACCTTAGGTTCTTTAGTCTTCCCTCTTCCACCAATTGCGTCTGACGATGGACCATTGTTAAGTGCTCAAGTCACAAGATTCAAATGTGGTGGCTTCGTTCTAGGTTTCGTCTTCCATCACTGTCTATTTGATGGTTTTGCCTTATCTGAATTTCTTAATGCATGGGCTGAGACAGCGTGTGGAGTCCCGTTATCAACCCCACCTGTTTTAGATAGAACATTTCCTCGTGCTAGATCTCCCCTGCAAATAAAGTACCCACATACAGAGTTCCTAGAGGTAGAAGATGTTTCATTAACTCAGAACATCTCTAACGATGCTATCAACAGATCCTTCTGTTTTACATCAGCCTCTTTGGAAATTTTGAAGAAGAAGGCGCTAGAAGACGGGGTGTTAAGCAAATGTACGACCTTCGAAGCTCTATCTGGCTTAATATGGAGAGCACGTACCAGAGCCTTATGGAGCGACTACCCCGAGCACAAGTTAAAGGTCCTGATTGTCGTGGACCCTCGTGCCAGATTTGAACCGAGAGTGGTGCCAAAAGGCTACGTAGGGAATGCAGTCTTGTTTACTTGTGCATTTGCTTCAGCCAGAGAACTAGAAGAAAATCCATTATCTCATGCTGTTAAACACGTACAGCACGCGATCGGCCGTATGACTGAGGAATATATGCTATCACAAATTGATTACATGGAGCACCAGAAGGTTTGGTGTCCCCCACTAGGTGCGTCGACTTCTTTTATGACTAAGTGGTCAAGGCTTGCCTTTAACATCTTAGATTTTGGTTGGGGTAGACCTAAGTATGTTGGTCCGGCTACTTCATTGTCTATGGAAACAACTACATTCGTCTCCTATGGAAAGGGTATGAGCGTGGTGTTAGCCCTACCCCCAGAGGCAATGAGAAAGTTCGAAAAGATTGTACACCCTTATTTGAACCCTTAA

SEQ ID NO:118

DfACT13蛋白质序列

MACGGGVRNIEVKAQEPVLVQPLSTAQSSAYNLLTKLDQTLAQLVVQIVFVFDVKNPVTRRPHDGADPAKLLKEALRKVLVPFYPLAGRLCLSPDDGSLFIDCNAQGVSFVEANADADISELGDFSQPDFATLGSLVFPLPPIASDDGPLLSAQVTRFKCGGFVLGFVFHHCLFDGFALSEFLNAWAETACGVPLSTPPVLDRTFPRARSPLQIKYPHTEFLEVEDVSLTQNISNDAINRSFCFTSASLEILKKKALEDGVLSKCTTFEALSGLIWRARTRALWSDYPEHKLKVLIVVDPRARFEPRVVPKGYVGNAVLFTCAFASARELEENPLSHAVKHVQHAIGRMTEEYMLSQIDYMEHQKVWCPPLGASTSFMTKWSRLAFNILDFGWGRPKYVGPATSLSMETTTFVSYGKGMSVVLALPPEAMRKFEKIVHPYLNP

SEQ ID NO:119

PYI04555.1天然核苷酸序列

ATGGGTGCCAGCGTCTCTTTCCAGCCCTTTGTGCCCACTCCCCTGGACCATGCTATGCCCCCAATTTACGTGTCTCAATTTCTATGCTTTCCTACCACAACTCCGCAATCCGCTATTCAAAGTCTCCAAGTGGGAATCGAGAGATTATTCGAACGCCTGCCATTCCTGGCGGGAGAGATTCTCATCAATGAACACACCGGAGCCATCAAAGTCCAGGCTCCCAGTGCTTTGATCCGGGAAATTCCCTACATGGCCCTCCGAGCCCATCCTGATCTTTACCTTCCAGCTAAGCAATGTGCAACCACACCAATCGAGAGACAGTTGAAGACCAACAGCCTTGATGAATCCTACCATCCACTCCCGGCGGCACTTCCACTCTCCCAACCCCAGCCCGTCATCCGATTCCAAGCAAACACCCTCGCAGACGGCATTCTCTTTGCAGTCAGCTATCATCATTGCATATTCGATGGCACTGGATGCGGACAGATTCTGGAAATGTTGGCTCAGTGCTGTTCGGCCTCCGACGATAAGATCTCCCTGCCGACTGATTGCCACACCGATGTGCTCCTCCGCGAATACATCTCCAATCTAAGCCCTACTACCAACATCCCCCACGATTACACGCAAGCGTATAGCACTACGGTGCAACCGGACCCCGATGCCTCAGACCCAGACACGTCCCCCGCCATACCCTCCTCACTCTACACAGAAGCATTCACCTTTCCCTCCCAACAAATCACCACTCTCCGTGATGCATGCAACCACCTCTTGCCCAAATTACCCAGCACCAGCAACGCACATCCCCACAAACCAACACCGAATCCCCTATCATCAAATGACGTCCTCACCGCACTAATAGCCCTATGCATCACACGCGCCACCAACACCACCACCCCACCCCTCCAACCCAACAATCACAGTCTCTCAATGGCCGTCAACCTCCGAACCCGCATCCAGCCCCAAGTACCAGATCACTTTCTGGGAAACTTCGCCACGCTACTCCCAATACACTTTACCAGTCCAGTCCACACCCAACAGTCTGATCTCCTCCTCACCACAGAACCCCCCGACCCCGCCCTCATCCACCTAACCACCCTCGCCTCCCAAATCCGGTCCAGCCTATCCACAGTCAACACCGATTACATCTGCGGTCTCATGACGGATCTCCGAACCCGGCGCAATGCAGGAGAAAACAGCAGTCTTCTAATTGAAGGCATTAAGATTTCCAGTTGGAGACATCTATCCGTCTACAAGCCGGACTTTGGCCCTGGGTTGGGGAAAATAGCGGGGTTTGAGTTCCAGGCGGGGCTTATGGATAATTTGGTGGTGATTTTGCCTTGGAGAAATGGGGATTGGGATGTGCGTGTTACGTTGTTGGAGAGGGATATGCGCGGGTTTAGGGAGGATCGGTTGGTTAGGTGGGCGTTGGGGTCTGGGTAG

SEQ ID NO:120

PYI04555.1核苷酸序列，针对其在酿酒酵母中的表达经密码子优化，编码SEQ IDNO:143的C-末端扩展蛋白变体。

ATGGGTGCGTCAGTTAGTTTTCAACCTTTCGTTCCTACCCCCTTGGATCACGCCATGCCACCGATATACGTGTCACAGTTCTTGTGTTTCCCTACTACGACGCCGCAGTCAGCAATACAATCCCTACAGGTCGGTATTGAGCGTTTGTTTGAAAGACTACCTTTTCTAGCCGGGGAAATTCTGATAAATGAGCACACAGGTGCAATAAAAGTTCAAGCTCCCTCTGCTCTGATTAGAGAAATTCCGTACATGGCCTTAAGGGCTCATCCGGATTTGTATCTGCCTGCTAAGCAATGTGCAACTACTCCAATAGAGAGGCAGTTGAAAACTAACAGCCTTGATGAGTCTTATCATCCATTACCTGCGGCACTACCATTGTCTCAACCACAACCAGTCATCAGATTCCAAGCTAATACACTTGCTGACGGAATACTTTTCGCTGTCAGTTACCACCATTGTATATTTGATGGAACGGGCTGCGGACAGATCCTTGAAATGTTAGCCCAGTGTTGTTCGGCCTCAGACGATAAGATCAGCTTGCCTACGGACTGTCATACAGATGTATTGTTGAGAGAATATATTTCGAATCTATCTCCAACCACGAATATCCCGCATGACTACACTCAAGCTTATTCTACTACAGTTCAACCAGATCCTGACGCATCCGATCCTGATACTAGCCCAGCCATTCCGAGTTCGCTGTACACTGAAGCATTTACGTTTCCTTCACAACAGATTACCACACTAAGAGATGCGTGCAACCATTTATTGCCTAAATTACCATCTACTTCAAACGCACATCCCCACAAACCAACACCAAACCCGTTATCCTCAAATGACGTTTTGACGGCACTGATCGCATTGTGCATCACCAGAGCTACTAATACGACGACACCCCCATTACAACCAAACAACCATTCTCTTAGCATGGCCGTCAATCTAAGGACTCGTATTCAACCCCAAGTCCCGGACCACTTTCTTGGTAATTTTGCCACCTTGCTACCTATTCACTTCACAAGTCCAGTCCATACGCAGCAATCAGATTTATTATTGACTACTGAGCCGCCAGACCCAGCATTGATCCACCTTACAACCCTTGCATCGCAAATTAGGTCTAGTTTATCGACCGTTAACACCGATTACATATGTGGCTTGATGACAGATCTGAGGACTAGGAGAAACGCCGGTGAGAATTCCTCTTTGTTAATAGAAGGTATTAAGATTTCTTCATGGAGACATCTTAGCGTTTACAAGCCAGATTTCGGACCTGGATTAGGGAAGATTGCAGGATTTGAGTTTCAAGCTGGTTTAATGGACAACCTTGTTGTGATATTGCCTTGGAGAAACGGTGATTGGGACGTGCGTGTAACTCTTCTGGAGAGAGATATGAGGGGTTTTAGGGAAGACAGATTAGTCAGATGGGCATTAGGTAGCGGAACAGGCCCCTTTTCCTTTGTCGATATCATGTAA

SEQ ID NO:121

PYI04555.1蛋白质序列，SEQ ID NO:143的C-末端扩展蛋白变体。

MGASVSFQPFVPTPLDHAMPPIYVSQFLCFPTTTPQSAIQSLQVGIERLFERLPFLAGEILINEHTGAIKVQAPSALIREIPYMALRAHPDLYLPAKQCATTPIERQLKTNSLDESYHPLPAALPLSQPQPVIRFQANTLADGILFAVSYHHCIFDGTGCGQILEMLAQCCSASDDKISLPTDCHTDVLLREYISNLSPTTNIPHDYTQAYSTTVQPDPDASDPDTSPAIPSSLYTEAFTFPSQQITTLRDACNHLLPKLPSTSNAHPHKPTPNPLSSNDVLTALIALCITRATNTTTPPLQPNNHSLSMAVNLRTRIQPQVPDHFLGNFATLLPIHFTSPVHTQQSDLLLTTEPPDPALIHLTTLASQIRSSLSTVNTDYICGLMTDLRTRRNAGENSSLLIEGIKISSWRHLSVYKPDFGPGLGKIAGFEFQAGLMDNLVVILPWRNGDWDVRVTLLERDMRGFREDRLVRWALGSGTGPFSFVDIM

SEQ ID NO:122

ERR364415-1_contig_8546天然核苷酸序列

ATGGCCCGGGCACCACCACCTCCGCCTCCTGGTCTCAGAATGAGAGACACAGTGCTCAGCATCGTGAAGCCGATACGGAAGACACAGCATTTGGAGACGATCGACGCAACGTTCGTCGATTTGATGAGAATGGACAGCTTTATACCCGTGATTTTCGCCTACAGGCCTGCGGACAAGTCCGAGGCCGCGTACTCGCGTCTCGTGAATCGCATCAAGGAGTCGCTCCAGAAGGTTTTGGTCCCCTTTTTCGGGTTCGCGGGCCGCTGGGTTCCAAGCAGTGGCGGGAGCAGGCGGCTCTTGTGCAACGATGAGGGCGTTCCCTTCATTGAAGCGTTTGTGGACGAAGAGTTGGACTCGGTGGTGAAGGCTTCCGCCGCATTCCAGCCGGTTACGGAGCTGAATGGCTTGGGCGTCCTCGGAATGGACATGACTTCATACGATCAAAGGATGCCACCGGAAGGTGGGCAACCTTGCGTCGTTGCTCAAGTCACACGGTTCAAATGTGGGGGAGTGGTTCTGGGGGTGGCTTTCAATCACACTCACACTGACGGCCAGGGATTCTACACCTTCATGCGAGCATGGTCCGACTTCTCTCGAACCAACGGAACGGCAATCAAGGTGGACCACAACCGGGCCCTGCCAGAACTGGCTTCCCTCTCACAGTTCTTCATCAAACAGCACGACCGAATAGGAGGCAAAACTTCTACCGATCGAGTCAACGATCATTGTTCTAAAGTTCCGGAACGGCTGGCTTTGAAAGCTTTCGAGGTTCGTGCGTCTAAGATCAAAGCCGCAAAGCTAGCAGCCGAAGATGGAGGGGTTGGGTATGTCAGCACGGTAGATTGCATTGTGGCTCACTTATGGAAAACTCTTGCCAGATTGCCGCCCGTCGTGTTGGATGGGAGGGAGATTACGGTCTTCTCGCCTGTGGAGGGGAGGAACAGATTCTTGGACCCGCCAAGACCCAATATGTGTGGAAATTGTTTTGCAGCAATGGTGACCCCCAAAATCCCAACCCAGGAGTTGCTGGAGATGCCTCTCGCTGCAATTGCAGGCAAGCAACGGGAGAAATTATCCACAACCCGAAGGGAGGAATGGTTTGGACAGCAAAGCTTTAGGGAGCTGGCCTCCGCGATGAACACCAGCAAATCTGCTCTACTTATTGTGACCTCGTGGTTCAACTTTCCCATGTATGAGATCGACTTTGGAGCTGGCAAACCATTTTTTGCATCCACTACGAACATGATTTCTCCTATCAACGGCGTGTGTTGTGGAGTCATTGCACCCCCAACTCCTGGGAGCTGCTCCTCCATTGCCACTCTGTACATTTTGTGCCTTCCCGCGGTACTAGAGGCTCTTGAAAATGTTCCAGATTTCCTATCCTTCTTCGTTCCTCACCCAAATCACAAAGATAACTCGCAATAG

SEQ ID NO:123

ERR364415-1_contig_8546核苷酸序列，针对其在酿酒酵母中的表达经密码子优化，编码SEQ ID NO:144的C-末端扩展蛋白变体。

ATGGCCAGGGCTCCACCCCCACCACCACCGGGCTTGCGTATGAGAGATACGGTCCTATCAATCGTCAAACCTATAAGAAAGACTCAACACCTAGAGACAATTGATGCAACATTCGTAGATTTAATGAGAATGGACTCATTTATCCCTGTGATCTTCGCTTATAGACCTGCAGATAAGAGCGAGGCTGCGTACTCTAGATTAGTTAATAGGATAAAAGAAAGCTTACAGAAAGTACTAGTACCATTCTTTGGATTTGCTGGAAGGTGGGTGCCTTCTTCCGGTGGCTCAAGGCGTCTGCTATGCAATGACGAAGGTGTCCCTTTTATTGAGGCGTTTGTAGACGAAGAACTGGACTCTGTCGTTAAGGCTTCAGCTGCCTTCCAACCTGTAACTGAACTTAATGGTCTAGGTGTGTTGGGTATGGATATGACTAGTTATGATCAAAGAATGCCCCCTGAAGGCGGTCAACCGTGCGTCGTAGCTCAGGTAACGAGATTTAAATGCGGCGGTGTGGTATTGGGTGTAGCATTCAACCATACCCATACTGACGGGCAGGGCTTTTATACTTTTATGCGTGCATGGTCGGATTTCAGTAGAACGAATGGTACCGCAATTAAAGTCGATCACAACAGGGCACTACCCGAATTAGCATCTTTGAGTCAGTTCTTTATTAAGCAGCATGATAGAATTGGTGGGAAAACTTCCACCGATAGAGTCAATGACCACTGTAGCAAAGTCCCTGAAAGACTAGCACTAAAGGCTTTTGAAGTTAGGGCGTCCAAAATCAAGGCAGCAAAACTAGCCGCAGAAGATGGTGGTGTAGGCTACGTCTCGACGGTGGATTGTATTGTTGCTCATCTATGGAAGACACTAGCTCGTTTGCCACCAGTTGTGTTAGACGGTCGTGAGATCACTGTGTTTAGCCCAGTAGAAGGCAGAAATAGGTTTCTTGATCCGCCCCGTCCGAACATGTGTGGTAATTGTTTCGCTGCAATGGTAACCCCTAAAATCCCAACACAAGAATTGTTAGAGATGCCATTAGCCGCCATTGCCGGCAAGCAGAGAGAGAAACTATCTACCACGAGACGTGAAGAGTGGTTCGGACAGCAATCATTCAGGGAGTTGGCTTCAGCTATGAATACTTCTAAATCAGCTTTGTTAATCGTGACATCTTGGTTTAACTTCCCGATGTATGAAATCGATTTTGGTGCCGGTAAGCCGTTCTTCGCTAGTACGACTAATATGATTTCTCCTATAAATGGAGTTTGTTGTGGTGTCATAGCCCCGCCCACCCCCGGTTCCTGTTCATCCATAGCGACATTATACATTTTATGTTTACCAGCCGTGTTAGAAGCTCTTGAAAATGTCCCAGATTTCCTTTCGTTCTTCGTACCGCATCCAAACCATAAAGACAACAGCCAAACAGGCCCCTTTTCCTTTGTCGATATCATGTAA

SEQ ID NO:124

ERR364415-1_contig_8546蛋白质序列，SEQ ID NO:144的C-末端扩展蛋白变体。

MARAPPPPPPGLRMRDTVLSIVKPIRKTQHLETIDATFVDLMRMDSFIPVIFAYRPADKSEAAYSRLVNRIKESLQKVLVPFFGFAGRWVPSSGGSRRLLCNDEGVPFIEAFVDEELDSVVKASAAFQPVTELNGLGVLGMDMTSYDQRMPPEGGQPCVVAQVTRFKCGGVVLGVAFNHTHTDGQGFYTFMRAWSDFSRTNGTAIKVDHNRALPELASLSQFFIKQHDRIGGKTSTDRVNDHCSKVPERLALKAFEVRASKIKAAKLAAEDGGVGYVSTVDCIVAHLWKTLARLPPVVLDGREITVFSPVEGRNRFLDPPRPNMCGNCFAAMVTPKIPTQELLEMPLAAIAGKQREKLSTTRREEWFGQQSFRELASAMNTSKSALLIVTSWFNFPMYEIDFGAGKPFFASTTNMISPINGVCCGVIAPPTPGSCSSIATLYILCLPAVLEALENVPDFLSFFVPHPNHKDNSQTGPFSFVDIM

SEQ ID NO:125

XP_001258079.1天然核苷酸序列

ATGACCGTGACCATCAGTTTCGAGCCATATGTGGGCTCCTCTGTCGATGCTCTAAGCATCCCTCTCTATCTTCGATGTCAACTCGTCTTCAAACTTTCTAAGCCACTTGCTGCGGTGCCTCTGCTTGAGTCTGGAGTTAATCGTCTTGTACAAGCGTTACCCTTCCTCTCGGGCGAGTTCACGGCCGTGCCAGCATCCGACGGTGGGAAAGAAATTCTTCTCGTTCGCCCTGTGCTCAACTTCGAGCTCAGCCGTATACTCAAGATCAAGTACCATGAAACATCCCTACGACATGTATGCAAACAGATGAACAGGCCAAGCAGCCAGGGTGGTGACCTTCCGCATGAGCCATACATGCCCTACCCACGACTTCCAGATCCTTCACGCCCTCAACCCATCGTCGGGTTCCAAGTCAACGTTCACACGGATGGCATCATTCTCTCCGTTGCTACGCATCACTGTTCCTTTGACGCAACAGGGATGGGATCAATCGTCCAAAACCTCGCGGCTTGTTGCCGTTCTCCTCCGAGCGACGAGCCTGACTTGACCACGTCGCCAGCCCAGGAAGCAGAAGCAAGAAAAGTCCTCTCGCAAGTCCGCGAGACGCCTTTTGATCCAAAGATGTTCCCGGAGTACAGACCCTTGGACAGTATGCTGTCCTATTACAAAGGCGTCCAGTCAGCGCTCCAGGGTCGTCAAACCACTATCGTCAATCGATGTTTCACAATCGCCGCCGACAAGATCAACGCGCTCAAAAGGCGCTGCAATCAACTGATACCGGAAATGGTGAAGAAGTATGGGCTTTCAACTGAGGATGCCATTGGGAGCGCCTGGGTCTCGAGTAATGATGTTGTTGCTGCCCTCTTGTGGACATGTATCAATCGAGCGCGATATCCCGAAATCCGCGAGCGCAGCGTTCACCAGCTCCCACCAGACCTCCTACATGCGACATCTAGCCTGGGTGTGCCAGTGAACGTTCGCTCTCGACTGTCGCCGCCCTTACCCAAATCGACTTTAGGTAACGCCGTGTGTCTTCTCCGGGAGAAGGTCCCGCTGCAATTTTTCGCTTTGCCTAGTCATGCCAACATGGAGGCCACTTCTAGCGTTTGCGCAGACCATTCCGGAGACGACGAATGGGCCTTGTCCTTCTGTCGAGTCGCCTACGGACTCAGAGCGAAGCTGAACGCAATTGATGACGACTATATCCGCGACTATATCTCCTACGTGCAAAAGTCTCCGTGCCATCTGTCAGTGACACTGGATACAGAGAACCTGTACCTCAGTAACTGGCGCGAGATCGGTGTGTATGATGCTGATTTTGGAGGCATGCTGGGCAAGCCGCTACGGATGAGAGCTCCGGATGGATACACCGATGGCCTGATTTTTGTGATGGCGCAGCGGAGCGAAGATAAGTCTGCACCGTGGGAGTTTAATATCTCGCTGGAGGCATCGACAATGAAGCGTATTGTGCATGATCCCCTCTGGTGCAAGTATGTTGAGCTGGATGCGTTCTGGCATGGAGAAGAATGA

SEQ ID NO:126

XP_001258079.1核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGACCGTCACGATTTCATTCGAACCATATGTGGGATCTAGCGTGGACGCGCTGTCCATACCCTTGTATCTAAGGTGCCAACTGGTGTTCAAATTATCCAAGCCTCTTGCAGCCGTGCCCCTGCTAGAATCTGGGGTAAACCGTCTAGTACAAGCATTACCATTCTTGTCTGGTGAATTTACTGCTGTCCCTGCAAGCGATGGTGGGAAGGAAATCTTACTTGTTAGACCAGTCTTAAATTTCGAACTAAGTCGTATACTGAAGATCAAATACCACGAAACATCCTTAAGACACGTATGCAAGCAGATGAACAGACCATCCTCGCAAGGTGGTGACTTGCCACACGAACCGTATATGCCATACCCCAGGTTACCAGATCCATCTAGGCCTCAACCAATCGTGGGTTTTCAAGTCAATGTCCACACTGACGGTATAATCCTGAGTGTAGCAACTCACCATTGCAGTTTTGACGCCACGGGGATGGGAAGTATTGTACAGAACTTGGCCGCATGCTGTAGATCTCCGCCATCGGACGAGCCAGATTTAACTACAAGCCCTGCTCAAGAAGCAGAAGCTAGGAAGGTCCTGAGCCAAGTTAGAGAAACACCATTCGACCCAAAGATGTTTCCCGAATATAGGCCCTTAGACTCTATGTTATCTTATTACAAAGGTGTCCAGTCTGCTTTGCAGGGTCGTCAAACTACTATCGTTAACAGATGTTTCACTATCGCTGCTGATAAGATAAACGCCTTAAAGAGGAGATGTAACCAACTTATTCCGGAAATGGTAAAGAAATACGGATTGAGTACAGAAGATGCTATCGGTTCTGCATGGGTCTCTTCTAATGACGTGGTTGCCGCACTTTTGTGGACCTGTATTAATCGTGCTAGATACCCAGAGATTAGAGAAAGAAGTGTACATCAGCTTCCACCAGACTTGTTGCATGCTACATCTTCATTGGGTGTACCTGTTAATGTTAGATCTAGACTTTCACCACCCTTACCCAAATCCACTCTTGGGAATGCGGTCTGCCTGCTAAGGGAGAAGGTACCTTTGCAATTCTTCGCTCTTCCGAGTCACGCAAACATGGAAGCAACCAGTTCAGTTTGCGCTGACCATTCAGGGGATGATGAATGGGCGTTGAGCTTTTGTAGGGTAGCTTATGGTCTGCGTGCAAAATTGAACGCGATCGATGATGATTACATAAGGGATTACATTAGTTATGTACAGAAGTCGCCCTGTCATCTTTCAGTTACACTAGATACTGAGAACTTATACCTGTCTAATTGGAGAGAAATAGGCGTCTACGATGCAGACTTCGGTGGTATGCTAGGGAAACCATTGCGTATGAGAGCTCCGGATGGCTACACTGACGGTTTGATTTTCGTTATGGCCCAAAGATCTGAAGACAAGTCAGCTCCGTGGGAATTCAACATATCCCTTGAGGCTTCTACAATGAAAAGGATTGTACATGATCCGCTGTGGTGTAAATATGTTGAATTGGATGCCTTTTGGCATGGGGAAGAGTAA

SEQ ID NO:127

XP_001258079.1蛋白质序列

MTVTISFEPYVGSSVDALSIPLYLRCQLVFKLSKPLAAVPLLESGVNRLVQALPFLSGEFTAVPASDGGKEILLVRPVLNFELSRILKIKYHETSLRHVCKQMNRPSSQGGDLPHEPYMPYPRLPDPSRPQPIVGFQVNVHTDGIILSVATHHCSFDATGMGSIVQNLAACCRSPPSDEPDLTTSPAQEAEARKVLSQVRETPFDPKMFPEYRPLDSMLSYYKGVQSALQGRQTTIVNRCFTIAADKINALKRRCNQLIPEMVKKYGLSTEDAIGSAWVSSNDVVAALLWTCINRARYPEIRERSVHQLPPDLLHATSSLGVPVNVRSRLSPPLPKSTLGNAVCLLREKVPLQFFALPSHANMEATSSVCADHSGDDEWALSFCRVAYGLRAKLNAIDDDYIRDYISYVQKSPCHLSVTLDTENLYLSNWREIGVYDADFGGMLGKPLRMRAPDGYTDGLIFVMAQRSEDKSAPWEFNISLEASTMKRIVHDPLWCKYVELDAFWHGEE

SEQ ID NO:128

XP_001217250.1天然核苷酸序列

ATGGCAACCTTCGACCATATCGAGGATGTCATCGGCCAGCTACCTATGCTGAAGAGCTACACCCATATCTTGCTGTGCTTTCCCCTCGCTGAGAGCCAACTCAATGAAGCCATCGAGAGCCTTGAATCTGCTGTACGTCAGGTTATAAAAACCTTTTCGTTCTTGGCTGGCAAAGTAGTCAACGAAGGCAAGGGACCCAACAGCTCGGGTACTTTCAGGGTCGCTCCCTGTGAGACGTGGGAGTCCCCAGATCATCAATTTGTGCGAGTCGTTGATCGCTCTTTCATGCTGGCCTCCTACGATGAGATCCGCGGAGCACAGGCACCTGCTTCCATGCTCCCAGGAAGTCAACTCGGGTATCGGGTGGCTTTTCCAGCGCATTATCACGAGACAGAGGACGATCCCGCGCCGGTCCTGGACATTCAGTGCAATTTGATACGGGGCGGACTGCTACTTGACATCGCTGCCCAACACAACATTATCGATGCGAGCGGTATCTTTCAAATTGCCAGCTTGATCGCTCTTTCCATGCGAGGCGAGTCAATTCCTGAGGATGTCATCAAGGAAGGGAACCGTGATCGACGCAATATCATTCCACTACTAGAGGCAGATGAGCCTCTTCTTGATCACAGCGAGCTCAAAGCCAGCAGCGCGGTGCAAAATCCGCCGCCCGTCAACTTCCTCCAGGGGTATAAATGGCAAATCTTCAAGCTCTCTGCCGAGGTGTTAACTCGAATTACCGCTGAAGGACGACGACAGCCACAGGAGTTTGTCCCCTCCGTCACATTTGTCTCGGCAAACGATTGCTTGACAGCCTTTCTGTGGCAACGGGTGATAGCCATGCGCCTGAAGCGGCTCCATACGCCCGAGGCCGTATCCAAACTGAGCCGTGCTGTTGATCTTCGGCGGGCGATGGGCATTACCCCAGCATACATGGGCCATATGATTCGTGTCGCAAATACTAGTCTCACTTTTCAAGAAATTGTGGCATGCTCTTTATCCAGGCTTGCATCTCTGCTCCGCAAGAGCATCATCGATGTCAGCCAGCCGTATGCGATTCGGAGCTACGTGACCTTTATTGCAAATGAGACGGATAAATCAAAGATTGCGTATGCGGGTGCTTTCAATCCTTGCACCGACATGTCGTGCTCGTCCATTGCACACATCACTGCTCCTGAATTCGGTCGTTTGGGAGCGCCTGACTTTATAAGGAGGCCTACCTACGGGCCTCTGCCGTGCTGCACCTATGTCGCTCCCGATAAGAACGATGGAGCTTTGGATCAAAACCAGGCATGGTCAGACGTTGTGAAGCGCATCGGTTGA

SEQ ID NO:129

XP_001217250.1核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGGCCACCTTCGACCACATTGAGGACGTGATAGGACAATTGCCCATGCTAAAGAGCTACACACATATTTTACTTTGCTTTCCGTTGGCAGAATCTCAACTGAACGAGGCGATTGAATCTTTGGAGTCAGCAGTCAGACAAGTAATTAAGACGTTTTCATTCCTTGCAGGTAAGGTTGTTAATGAAGGCAAAGGTCCGAATTCCTCTGGTACGTTCCGTGTTGCTCCGTGTGAAACTTGGGAATCTCCTGATCATCAATTTGTTCGTGTTGTAGATAGGAGCTTTATGTTGGCCTCGTATGATGAGATCCGTGGTGCTCAAGCCCCCGCTTCCATGTTACCTGGGTCACAACTGGGGTATAGGGTAGCATTCCCAGCCCATTATCATGAAACTGAAGACGATCCAGCTCCAGTTCTAGATATCCAGTGTAATTTGATAAGAGGTGGTCTGCTTCTAGATATAGCGGCCCAACACAACATAATTGATGCTTCCGGGATATTTCAAATCGCTAGTCTGATCGCCTTAAGCATGAGAGGAGAAAGTATCCCTGAAGATGTTATCAAAGAAGGAAACAGAGATAGAAGGAATATCATTCCGTTATTGGAAGCCGATGAGCCTTTATTAGACCATAGTGAATTGAAGGCATCCAGCGCCGTTCAGAACCCGCCACCAGTTAATTTCTTGCAAGGTTATAAATGGCAGATTTTCAAACTGTCCGCTGAGGTATTGACCCGTATTACTGCCGAAGGACGTAGACAACCACAAGAATTTGTGCCCTCAGTGACCTTTGTGTCCGCAAACGACTGCTTGACTGCTTTCTTGTGGCAGCGTGTGATAGCTATGAGGCTAAAGAGATTGCATACCCCCGAGGCCGTTTCCAAGCTATCTAGAGCCGTGGATTTAAGAAGGGCCATGGGTATAACCCCTGCATACATGGGCCATATGATAAGAGTCGCAAACACATCCTTAACCTTTCAAGAAATTGTCGCATGTAGTTTATCACGTCTTGCGAGCTTACTTAGAAAATCTATCATTGATGTGTCCCAACCATATGCGATCAGATCATACGTCACCTTCATAGCAAACGAAACAGACAAATCTAAGATAGCCTATGCCGGGGCATTTAACCCGTGTACTGATATGTCATGTTCATCCATAGCTCATATTACGGCACCGGAGTTTGGTAGGTTAGGGGCGCCTGACTTCATTAGGAGACCCACTTATGGCCCACTACCTTGCTGTACTTACGTAGCTCCTGACAAGAATGACGGTGCATTGGATCAGAATCAAGCATGGTCAGATGTCGTAAAGAGGATAGGTTAA

SEQ ID NO:130

XP_001217250.1蛋白质序列

MATFDHIEDVIGQLPMLKSYTHILLCFPLAESQLNEAIESLESAVRQVIKTFSFLAGKVVNEGKGPNSSGTFRVAPCETWESPDHQFVRVVDRSFMLASYDEIRGAQAPASMLPGSQLGYRVAFPAHYHETEDDPAPVLDIQCNLIRGGLLLDIAAQHNIIDASGIFQIASLIALSMRGESIPEDVIKEGNRDRRNIIPLLEADEPLLDHSELKASSAVQNPPPVNFLQGYKWQIFKLSAEVLTRITAEGRRQPQEFVPSVTFVSANDCLTAFLWQRVIAMRLKRLHTPEAVSKLSRAVDLRRAMGITPAYMGHMIRVANTSLTFQEIVACSLSRLASLLRKSIIDVSQPYAIRSYVTFIANETDKSKIAYAGAFNPCTDMSCSSIAHITAPEFGRLGAPDFIRRPTYGPLPCCTYVAPDKNDGALDQNQAWSDVVKRIG

SEQ ID NO:131

BAU61551.1天然核苷酸序列

ATGTCCAAGCCCTTATTCGAAGCGTATCCTCTCACAGGGCTTGATCATACGATTCCTCCATGTTATGTTCGCTTCCTTCTAACTTTCCCCGTGCCGGATGTGGCATTGGCTGTCAATCAGCTGCAAAAGGGAGCTGAAAATTTAATCGAGAAACTTCCTTTCCTGGCCGGATATTTGGCTTCATGCGAGACCCCCGGCGTACGCCCAGGGCAGCTCGAGATTCGACCTCCCGCTGGGGAAAGGAGACCTGTCTGCCTCGTCGCACATCACTCGAACTCCTATCTCGCAGATTCCAGTGCGACGTCGACGACGGAACAGCTGGGCACCGCCAACGAGAACTATCTCCCTGTCCCGTTCTTCCCGGAGCTAGACAAGCCGGTGCCCATCTTCCGGGTTAAGGTGAATGCCATGACGGACGGCATCATTCTGGGATTTGCGTTCCACCATAGCGTGATCGATGCCACCGGGATGGGCACCATTGTCCGGGACTTTGCCAGATGCTGCCGTGGCCCTGATGGCGGTCCCCTGGAAATCAGTCTGGAGTCTCAGCAGGACTCTAGAGAGAAGCTGAGACACTCCGGAGGACCTCCCGATCCGCGGTTTGACCACAATGGGGAGTACCCTCTCGTGGCGTCTCTGCCCGCCGACCTCGAAGCCATGAAGCAGGTCTTGATCCAGACGGCCCGTCTCATGTCAACGCAGTATTTCCGCATCCCTGCCAGCCTAGTTAACACGCTAAAGGAATCCTGCAATCGGATGCTTCGGGAATCACCAGCGCTCAGGGACGAAGGGGAGAATCCATGGATTTCGAGCAACGATCTGGTGGTGTCGCTGTTGTGGCTGTGTCTGAATCGCGTTCGGTATCCTGAAGATAATACCAACGTCATTCCTCCTTCCGATTCCTCGGTCTGCATGGCCGTGAATATCCGAGGGCGTTTGCAGTCGCCCATTGATCCAGGATACGTTGGCAACGCCATCGTCCTTCTCCGGGAGAGCGTTGGCATGAATGCTTTTCTGCATAAACCGGGCGACGATGATCCCCTGGGCGCCCAATGTTACGAAACAGCGAAACGGCTAGGCCGAGAAGCGTGGGAAGCAGCCCTGGTGCGCATCGCCCTGGCCATCCGCCGCAAGCTCAACACCATAAACGCGAGTTACGTGCGCAGTGTTATATCCTATCTGGAGGACGTGCCCGACCTGTCCACTGTGGCGTTTGGCCAGACGGACTACCACATCAGCAGCTGGCGGGATATTGGCGTCTACGAGGCTGATTTTGGTGGCCACATGGGCCATCCCAGCGAAATGCGAGTACCAGATGGGATGGTCGATGGCATGTTTTACATCTTACCTCGAAGGCAGGGAACACACCCTTGCTGGGAGATCCATGTTACTATCCACCAGGACACAATGAAGCGACTCATTGCAGACCCTGTGTGGGCACGATATACAGTGAGAAAGCCTTCATCACTCTGCCGGGATGAATGA

SEQ ID NO:132

BAU61551.1核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGTCAAAGCCTCTGTTTGAAGCATACCCCCTAACTGGTCTAGATCATACTATACCCCCTTGTTACGTCAGGTTCTTATTAACTTTCCCAGTTCCAGATGTAGCCCTTGCTGTTAACCAATTACAGAAAGGCGCCGAAAATTTAATAGAGAAATTGCCTTTTCTTGCAGGGTACCTAGCTAGCTGTGAAACACCTGGCGTAAGACCGGGGCAGTTAGAAATCAGGCCACCAGCAGGTGAAAGAAGACCAGTGTGTTTGGTCGCACATCACAGTAACTCGTACCTTGCGGATTCTTCTGCAACAAGTACGACGGAGCAATTGGGAACAGCTAACGAAAATTACCTTCCCGTACCATTCTTTCCAGAGTTGGACAAACCTGTTCCAATATTCCGTGTCAAAGTTAACGCTATGACAGATGGGATTATCTTGGGGTTCGCTTTCCACCATAGTGTAATAGATGCTACGGGAATGGGTACAATAGTTAGGGACTTCGCCAGGTGCTGCAGAGGTCCTGATGGTGGGCCCTTAGAGATAAGTCTAGAAAGCCAACAAGATTCGAGAGAAAAGCTGAGGCACTCAGGCGGTCCGCCAGATCCCAGATTCGATCATAACGGAGAATACCCATTGGTGGCCTCACTGCCAGCGGACTTAGAAGCTATGAAACAAGTTTTAATCCAAACAGCGAGGCTGATGAGTACACAATACTTTAGAATACCTGCTAGCCTTGTGAACACTTTAAAAGAGTCATGTAATAGAATGCTTCGTGAATCCCCTGCACTGAGGGATGAAGGTGAAAACCCGTGGATTAGTTCTAACGATTTAGTAGTGAGTCTACTGTGGCTTTGTTTGAACAGGGTGAGGTACCCCGAAGACAATACAAATGTGATTCCACCCTCTGACAGTTCTGTTTGCATGGCTGTAAATATAAGAGGGAGATTACAGTCGCCGATCGATCCAGGTTATGTTGGTAATGCTATTGTATTATTAAGAGAATCTGTTGGAATGAATGCCTTTCTACATAAACCTGGTGATGACGACCCGCTTGGTGCCCAATGTTACGAGACAGCTAAAAGACTTGGAAGAGAAGCATGGGAAGCAGCATTAGTCAGGATTGCTTTGGCAATTAGGCGTAAGTTGAACACTATTAATGCTTCCTATGTCAGATCAGTTATTAGCTACTTAGAGGATGTGCCCGATCTATCAACCGTTGCCTTCGGGCAAACTGATTATCATATCTCCAGTTGGAGAGACATTGGAGTTTACGAGGCTGACTTTGGTGGTCATATGGGCCATCCATCTGAGATGAGAGTCCCTGACGGGATGGTCGATGGTATGTTTTACATACTACCTAGAAGACAAGGTACTCACCCATGTTGGGAAATTCATGTGACTATACACCAGGATACCATGAAAAGACTGATCGCTGATCCGGTTTGGGCAAGATATACCGTTAGAAAGCCTAGTTCTTTGTGCAGGGACGAGTAA

SEQ ID NO:133

BAU61551.1蛋白质序列

MSKPLFEAYPLTGLDHTIPPCYVRFLLTFPVPDVALAVNQLQKGAENLIEKLPFLAGYLASCETPGVRPGQLEIRPPAGERRPVCLVAHHSNSYLADSSATSTTEQLGTANENYLPVPFFPELDKPVPIFRVKVNAMTDGIILGFAFHHSVIDATGMGTIVRDFARCCRGPDGGPLEISLESQQDSREKLRHSGGPPDPRFDHNGEYPLVASLPADLEAMKQVLIQTARLMSTQYFRIPASLVNTLKESCNRMLRESPALRDEGENPWISSNDLVVSLLWLCLNRVRYPEDNTNVIPPSDSSVCMAVNIRGRLQSPIDPGYVGNAIVLLRESVGMNAFLHKPGDDDPLGAQCYETAKRLGREAWEAALVRIALAIRRKLNTINASYVRSVISYLEDVPDLSTVAFGQTDYHISSWRDIGVYEADFGGHMGHPSEMRVPDGMVDGMFYILPRRQGTHPCWEIHVTIHQDTMKRLIADPVWARYTVRKPSSLCRDE

SEQ ID NO:134

PsSalAT天然核苷酸序列

ATGGCAACAATGTATAGTGCTGCTGTTGAAGTGATCTCTAAGGAAACCATTAAACCCACAACTCCAACCCCATCTCAACTTAAAAACTTCAATCTGTCACTTCTCGATCAATGTTTTCCTTTATATTATTATGTTCCAATCATTCTTTTCTACCCAGCCACCGCCGCTAATAGTACCGGTAGCAGTAACCATCATGATGATCTTGACTTGCTTAAGAGTTCTCTTTCCAAAACACTAGTTCACTTTTATCCAATGGCTGGTAGGATGATAGACAATATTCTGGTCGACTGTCATGACCAAGGGATTAACTTTTACAAAGTTAAAATTAGAGGTAAAATGTGTGAGTTCATGTCGCAACCGGATGTGCCACTAAGCCAGCTTCTTCCCTCTGAAGTTGTTTCCGCGAGTGTCCCTAAGGAAGCACTGGTGATCGTTCAAGTGAACATGTTTGACTGTGGTGGAACAGCCATTTGTTCGAGTGTATCACATAAGATTGCCGATGCAGCTACAATGAGTACGTTCATTCGTAGTTGGGCAAGCACCACTAAAACATCTCGTAGTGGGGGTTCAACTGCTGCCGTTACAGATCAGAAATTGATTCCTTCTTTCGACTCGGCATCTCTATTCCCACCTAGTGAACGATTGACATCTCCATCAGGGATGTCAGAGATACCATTTTCCAGTACCCCAGAGGATACAGAAGATGATAAAACTGTCAGCAAGAGATTTGTGTTCGATTTTGCAAAGATAACATCTGTACGTGAAAAGTTGCAAGTATTGATGCATGATAACTACAAAAGCCGCAGGCAAACAAGGGTTGAGGTGGTTACTTCTCTAATATGGAAGTCCGTGATGAAATCCACTCCAGCCGGTTTTTTACCAGTGGTACATCATGCCGTGAACCTTAGAAAGAAAATGGACCCACCATTACAAGATGTTTCATTCGGAAATCTATCTGTAACTGTTTCGGCGTTCTTACCAGCAACAACAACGACAACAACAAATGCGGTCAACAAGACAATCAATAGTACGAGTAGTGAATCACAAGTGGTACTTCATGAGTTACATGATTTTATAGCTCAGATGAGGAGTGAAATAGATAAGGTCAAGGGTGATAAAGGTAGCTTGGAGAAAGTCATTCAAAATTTTGCTTCTGGTCATGATGCTTCAATAAAGAAAATCAATGATGTTGAAGTGATAAACTTTTGGATAAGTAGCTGGTGCAGGATGGGATTATACGAGATTGATTTTGGTTGGGGAAAGCCAATTTGGGTAACAGTTGATCCAAATATCAAGCCGAACAAGAATTGTTTTTTCATGAATGATACGAAATGTGGTGAAGGAATAGAAGTTTGGGCGAGCTTTCTTGAGGATGATATGGCTAAGTTCGAGCTTCACCTAAGTGAAATCCTTGAATTGATTTGA

SEQ ID NO:135

PsSalAT核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGGCAACAATGTACTCAGCTGCAGTTGAGGTTATATCTAAGGAAACGATAAAACCAACCACTCCAACCCCAAGCCAATTGAAGAATTTCAATTTATCTTTATTAGACCAGTGCTTTCCCTTGTACTACTATGTCCCCATCATCTTGTTCTACCCTGCGACTGCTGCAAACTCCACTGGTTCCTCGAACCACCATGATGATCTAGATCTTCTGAAGAGCTCCCTTAGCAAGACACTTGTTCACTTCTACCCTATGGCCGGTAGGATGATCGATAACATATTGGTTGACTGCCACGATCAGGGTATCAATTTCTATAAAGTTAAAATCAGGGGCAAGATGTGTGAATTCATGTCTCAGCCTGATGTGCCACTGTCGCAGCTGCTACCTAGTGAAGTGGTATCCGCATCTGTCCCAAAAGAGGCCTTGGTCATAGTCCAAGTTAATATGTTCGATTGCGGTGGGACGGCCATCTGCTCGTCGGTCAGTCATAAGATCGCAGACGCCGCAACCATGTCAACATTTATTAGATCTTGGGCGAGTACCACCAAAACTTCAAGGTCTGGCGGGTCAACCGCCGCTGTTACTGACCAGAAGTTGATTCCTAGCTTTGATTCGGCAAGCTTATTCCCACCTTCCGAAAGGTTGACTTCACCAAGCGGGATGTCTGAGATACCATTTTCTTCAACCCCTGAAGATACCGAGGACGACAAAACAGTTAGCAAAAGATTCGTGTTTGACTTTGCAAAGATAACATCTGTTAGAGAAAAGCTTCAGGTATTAATGCACGACAACTACAAAAGCAGGCGTCAGACCAGGGTTGAGGTCGTAACGAGCCTGATCTGGAAGAGTGTCATGAAGTCAACACCAGCTGGGTTCCTTCCCGTCGTGCATCATGCGGTAAATTTGAGGAAGAAGATGGACCCACCATTGCAGGATGTCTCCTTCGGCAACCTGAGTGTTACTGTCTCAGCATTCTTGCCCGCCACAACTACCACCACCACAAATGCCGTTAATAAGACAATAAATTCAACTTCATCGGAGAGTCAGGTGGTGCTACATGAATTGCACGATTTTATCGCACAAATGAGAAGTGAGATAGACAAAGTTAAAGGCGATAAGGGTAGCCTAGAAAAGGTGATTCAGAACTTTGCCTCTGGTCATGACGCTTCAATAAAGAAAATAAATGACGTAGAGGTCATAAATTTCTGGATATCTTCATGGTGCAGAATGGGCTTGTATGAGATCGACTTCGGCTGGGGCAAGCCGATTTGGGTTACAGTTGACCCTAATATCAAGCCAAATAAGAATTGCTTCTTTATGAACGACACAAAATGCGGAGAAGGAATAGAGGTATGGGCAAGCTTCCTGGAAGACGACATGGCCAAGTTTGAATTGCACTTATCGGAGATATTGGAATTGATCTAA

SEQ ID NO:136

PsSalAT蛋白质序列

MATMYSAAVEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDLDLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVPLSQLLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKSTPAGFLPVVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWCRMGLYEIDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFELHLSEILELI

SEQ ID NO:137

AstC核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGACTAAGATCAACCCATACAAGGGTATCTTGGTTGAATTGAAGGACATCGTTTTCACTTCTTCTTCTGACCAAATCAAGTTGCCAATCAACACTTTCAAGTCTATCTTGTGTTGTGGTGCTACTGCTCAATACCAATGTGGTAAGATCAACAGAGCTCAATACTACTCTAGATTGGCTAGAGACTTCGCTTTGTCTTTGGCTGACGTTACTGCTTTGTTCGACACTGTTCAAGCTACTATCAGACCAGAAGAATCTTTCTTGGCTTTCTTGGCTGAATTGAAGTCTAGATTCGGTGAACAATTGAAGTTGTACGCTGTTGCTAACATGTCTAGAGAAGACTACGCTATGTTGAAGTCTTTGCCAATCGACTGGTCTTTGTTCGACGGTGTTTTCTTGTCTGCTGACTTGGGTATGAGAAAGCCAGAATTGAGATTCTTCAGACACGTTTTGGAATCTATCTCTATGAAGCCAGAAGACACTATCTTGGTTGACAACGACACTGACAACATCTTGTGTGCTTTGTCTATGGGTTTGAAGGGTATCTTGTTCGGTTCTACTTCTGTTCCACAAGCTTTGACTAACTTGTTGGAATACGACCACATCTCTAGAGCTGAACAATTCTTGAGATCTCACGCTAAGTCTTTGCACTCTGTTACTCACACTGGTGTTACTATCAGAGAAAACTTCGCTCAATTGTTGATCTTGGAAGCTACTGGTGACATCGACTTGGTTGAATTGGAATACCACCCAACTACTTGGAACTACTTCATCGGTACTCCAGTTTTGACTCAAACTGAATTCCCACACGACTTGGACACTACTTCTTTGGCTACTACTGTTTTGGACAGACCAAAGGACATCGCTAACGAAATCATGGACGAAATGTTGAAGTACAGATCTGACGACGACTTGATGTTGACTTTCTTCACTGACTTCAAGAACAGAGTTGACCCAGTTGTTTGTTGTAACGTTTTGTCTTTGTTCTACAAGTACGGTAGAGGTCACGAATTGCACCACACTTTGGCTTGGGTTAGACAAGTTTTGATCAGAAGAGCTTACATCAACGGTACTGCTTTCTACCCAATGCCAGAAGCTTTCTTGTACTTCTTCTTCAGATTCTTGCAACACATCACTCACTTGCCACAATTGTACGACGGTTTGAAGGTTTTGTTGAAGGAAAGATTGCAAGAAAGAGTTGGTGTTCCAGTTGACCCAATCTCTTTGTCTATGAGATTGATCGCTTGTAACGGTGTTGGTATCCACGACAGAATGGGTTTGAACGCTTTGTTGTCTATGCAAAACCCAGACGGTTCTTGGGACTTGGGTACTATGTACCACTACGCTTCTAAGAGATTGCCAATCGGTAACCAAGGTGTTTCTACTGCTATGGCTATCAAGGCTATCAAGCAATGTCAAGCTAACCAATGTGCTGGTATCTAA

SEQ ID NO:138

AstC蛋白质序列

MTKINPYKGILVELKDIVFTSSSDQIKLPINTFKSILCCGATAQYQCGKINRAQYYSRLARDFALSLADVTALFDTVQATIRPEESFLAFLAELKSRFGEQLKLYAVANMSREDYAMLKSLPIDWSLFDGVFLSADLGMRKPELRFFRHVLESISMKPEDTILVDNDTDNILCALSMGLKGILFGSTSVPQALTNLLEYDHISRAEQFLRSHAKSLHSVTHTGVTIRENFAQLLILEATGDIDLVELEYHPTTWNYFIGTPVLTQTEFPHDLDTTSLATTVLDRPKDIANEIMDEMLKYRSDDDLMLTFFTDFKNRVDPVVCCNVLSLFYKYGRGHELHHTLAWVRQVLIRRAYINGTAFYPMPEAFLYFFFRFLQHITHLPQLYDGLKVLLKERLQERVGVPVDPISLSMRLIACNGVGIHDRMGLNALLSMQNPDGSWDLGTMYHYASKRLPIGNQGVSTAMAIKAIKQCQANQCAGI

SEQ ID NO:139

AstI核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGACTAGACAATCTCACTACCAAGCTATCATCTTGGACTTGGGTAACGTTGTTTTCGAATGGGACACTTCTCAAAACCCACCAACTGCTGCTCCAAACCAAATCTCTTTGTTGAGAACTTCTATGAAGTCTCCAGTTTACCACTCTTACGAAAGAGGTCAATTGTCTACTGAAGAATGTCACAGATTGTTGGGTGAATCTTTGCACGTTGACCCAGGTCAAATCAAGGAAGCTTTCGACTTGGCTAGACAATCTTTGAGATCTAACCCAGCTTTGTTGGACTTCATCAGACAATTGAAGCAAACTAGAGGTGTTGCTGTTTACGCTATGTCTAACATCCCACAAGCTGAAATCGAATACTTGAAGGAATCTAGAGCTGGTGACATGGAAGTTTTCGACGAAGTTTTCGCTTCTGGTTACGTTGGTTCTAGAAAGCCAGAAACTGAATTCTACAGAAGAGTTATGGGTGAAATCGGTTTGAAGGCTGAAAGAGTTGTTTTCGTTGACGACAAGGAAGAAAACGTTGACGTTGCTAGAGGTTTGGGTTTGTACGGTGTTTGTTTCGGTGGTGTTGAAGAATTGAGAGGTCACTTGTTGGGTATCTAA

SEQ ID NO:140

AstI蛋白质序列

MTRQSHYQAIILDLGNVVFEWDTSQNPPTAAPNQISLLRTSMKSPVYHSYERGQLSTEECHRLLGESLHVDPGQIKEAFDLARQSLRSNPALLDFIRQLKQTRGVAVYAMSNIPQAEIEYLKESRAGDMEVFDEVFASGYVGSRKPETEFYRRVMGEIGLKAERVVFVDDKEENVDVARGLGLYGVCFGGVEELRGHLLGI

SEQ ID NO:141

AstK核苷酸序列，针对其在酿酒酵母中的表达经密码子优化。

ATGTGTACTACTTTCAAGGCTGCTATCTTCGACATGGGTGGTGTTTTGTTCACTTGGAACCCAATCGTTGACACTCAAGTTTCTTTGAAGGACTTGGGTACTATCATCAACTCTGAAACTTGGGAACAATTCGAAAGAGGTAAGATCGAACCAGACGACTGTTACCACCAATTGGGTTCTCAAATCGGTTTGCCAGGTTCTGAAATCGCTGCTACTTTCAGACAAACTACTGGTTGTTTGAGACCAGACGCTAGAATGACTTCTTTGTTGAGAGAATTGAAGGGTCAAGGTGTTGCTGTTTACATGATGACTAACATCCCAGCTCCAGACTTCCACCAATTGAGAGAAATGCACTACGAATGGGACTTGTTCGACGGTATCTTCGCTTCTGCTTTGGAAGGTATGAGAAAGCCAGACTTGGAATTCTACGAACACGTTTTGAAGCAAATCGACACTTCTGCTGCTGAAACTATCTTCGTTGACGACAAGTTGGAAAACGTTATCGCTGCTCAAGCTGTTGGTATGGTTGGTTTGCACTTGACTGACTCTTTGGCTACTTGTATGGAATTGAGACAATTGGTTGGTTGTTAA

SEQ ID NO:142

AstK蛋白质序列

MCTTFKAAIFDMGGVLFTWNPIVDTQVSLKDLGTIINSETWEQFERGKIEPDDCYHQLGSQIGLPGSEIAATFRQTTGCLRPDARMTSLLRELKGQGVAVYMMTNIPAPDFHQLREMHYEWDLFDGIFASALEGMRKPDLEFYEHVLKQIDTSAAETIFVDDKLENVIAAQAVGMVGLHLTDSLATCMELRQLVGC

SEQ ID NO:143

PYI04555.1蛋白质序列，未经C-末端扩展。

MGASVSFQPFVPTPLDHAMPPIYVSQFLCFPTTTPQSAIQSLQVGIERLFERLPFLAGEILINEHTGAIKVQAPSALIREIPYMALRAHPDLYLPAKQCATTPIERQLKTNSLDESYHPLPAALPLSQPQPVIRFQANTLADGILFAVSYHHCIFDGTGCGQILEMLAQCCSASDDKISLPTDCHTDVLLREYISNLSPTTNIPHDYTQAYSTTVQPDPDASDPDTSPAIPSSLYTEAFTFPSQQITTLRDACNHLLPKLPSTSNAHPHKPTPNPLSSNDVLTALIALCITRATNTTTPPLQPNNHSLSMAVNLRTRIQPQVPDHFLGNFATLLPIHFTSPVHTQQSDLLLTTEPPDPALIHLTTLASQIRSSLSTVNTDYICGLMTDLRTRRNAGENSSLLIEGIKISSWRHLSVYKPDFGPGLGKIAGFEFQAGLMDNLVVILPWRNGDWDVRVTLLERDMRGFREDRLVRWALGSG

SEQ ID NO:144

ERR364415-1_contig_8546蛋白质序列，未经C-末端扩展。

MARAPPPPPPGLRMRDTVLSIVKPIRKTQHLETIDATFVDLMRMDSFIPVIFAYRPADKSEAAYSRLVNRIKESLQKVLVPFFGFAGRWVPSSGGSRRLLCNDEGVPFIEAFVDEELDSVVKASAAFQPVTELNGLGVLGMDMTSYDQRMPPEGGQPCVVAQVTRFKCGGVVLGVAFNHTHTDGQGFYTFMRAWSDFSRTNGTAIKVDHNRALPELASLSQFFIKQHDRIGGKTSTDRVNDHCSKVPERLALKAFEVRASKIKAAKLAAEDGGVGYVSTVDCIVAHLWKTLARLPPVVLDGREITVFSPVEGRNRFLDPPRPNMCGNCFAAMVTPKIPTQELLEMPLAAIAGKQREKLSTTRREEWFGQQSFRELASAMNTSKSALLIVTSWFNFPMYEIDFGAGKPFFASTTNMISPINGVCCGVIAPPTPGSCSSIATLYILCLPAVLEALENVPDFLSFFVPHPNHKDNSQ

适用于本发明的其他序列在下面列出。“先前的”SEQ ID NO是指在相应专利文件中使用的那些。

1.PCT/CN2018/088902(2018年5月29日提交)中所述的折叶苔基二磷酸合酶为：

香鳞毛蕨(Dryopteris fragrans)的DfHAD、DfHAD-9(V274A)和DfHAD-8(K532R)

NA＝核酸

AA＝氨基酸

2.PCT/EP2018/064344(2018年5月31日提交)中所述的补身烷合酶(即折叶苔醇合酶或补身醇合酶)是

AA＝氨基酸

3.WO2015/169871中所述的补身醇合酶是

4.WO 2015/176959中所述的补身醇合酶是

黑水缬草(Valeriana amurensis)的VaTPS3(SEQ ID NO:6)

序列表

<110> 弗门尼舍有限公司

<120> 用于产生乙酸补身烷基酯化合物的方法

<130> 11110/WO

<160> 144

<170> PatentIn version 3.5

<210> 1

<211> 5689

<212> DNA

<213> 质粒 pF167

<400> 1

tggtcagcaa caacgccgaa gaatcactct cgtgttgaga attgcacgcc ttgaccacga 60

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 120

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 180

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 240

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 300

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 360

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 420

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 480

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 540

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 600

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 660

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 720

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 780

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 840

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 900

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 960

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 1020

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 1080

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 1140

cgcgcacatt tttggcattg gcaaagtgcg gactgcatag tcactgtggt gccgtactta 1200

gggtacgcgt tcctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg 1260

agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac 1320

gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca 1380

acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat 1440

gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa 1500

atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt 1560

gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga 1620

agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc 1680

gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt 1740

atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga 1800

ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta 1860

taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca 1920

atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta 1980

gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga 2040

tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag 2100

tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt 2160

ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg 2220

aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata 2280

cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag 2340

aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag 2400

gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg 2460

cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc 2520

tcatccttca atgctatcat tatgtcaccc gcagttctgt gtcgtagtca tcaacatagc 2580

acctatcctt tggcatctcg gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 2640

gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 2700

cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 2760

ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 2820

tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 2880

gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 2940

tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 3000

ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 3060

ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 3120

ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 3180

accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 3240

tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagcactt gctacactgt 3300

caggatagct tccgtcacat ggtggcgatc accgtacatc tgagtgagac gttaattaaa 3360

gtagaccgct cacacatggg cggccgccgt ctcaaggtgc agttcgcgtg caattataac 3420

gtcgtggcaa ctgttatcag tcgtaccgcg ccattcgact acgtcgtaag gccgtttctg 3480

acagagtaaa attcttgagg gaactttcac cattatggga aatgcttcaa gaaggtattg 3540

acttaaactc catcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt 3600

ttttagagaa aatcctccaa tatcaaatta ggaatcgtag tttcatgatt ttctgttaca 3660

cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattcttt 3720

ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tactatcctc 3780

ctttttctcc ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga 3840

acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat 3900

gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc 3960

gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc 4020

tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa 4080

tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggtcaa ccttattctt 4140

tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc 4200

tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc 4260

ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt 4320

tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt 4380

agggaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa 4440

aagattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa 4500

agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc 4560

gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct 4620

gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag 4680

agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat 4740

ttttagtaaa ccttgttcag gtctaacact accggtaccc catttaggac cagccacagc 4800

acctaacaaa acggcatcaa ccttcttgga ggcttccagc gcctcatctg gaagtgggac 4860

acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt 4920

ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc 4980

aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacatagggg cagacattag 5040

aatggtatat ccttgaaata tatatatata ttgctgaaat gtaaaaggta agaaaagtta 5100

gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg 5160

tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa 5220

aagccggttc cggcctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat 5280

atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg 5340

cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat 5400

tcgaactctt gcatcttacg atacctgagt attcccacag ttaactgcgg tcaagatatt 5460

tcttgaatca ggcgccttag accgctcggc caaacaacca attacttgtt gagaaataga 5520

gtataattat cctataaata taacgttttt gaacacacat gaacaaggaa gtacaggaca 5580

attgattttg aagagaatgt ggattttgat gtaattgttg ggattccatt tttaataagg 5640

caataatatt aggtatgtgg atatactaga agttctcctc gaccgtcga 5689

<210> 2

<211> 60

<212> DNA

<213> 用于同源重组的序列

<400> 2

gcacttgcta cactgtcagg atagcttccg tcacatggtg gcgatcaccg tacatctgag 60

<210> 3

<211> 60

<212> DNA

<213> 用于同源重组的序列

<400> 3

aggtgcagtt cgcgtgcaat tataacgtcg tggcaactgt tatcagtcgt accgcgccat 60

<210> 4

<211> 1584

<212> DNA

<213> XP_007369631.1 污叉丝孔菌(Dichomitus squalens)折叶苔醇合酶，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1584)

<400> 4

atg gct tct atc cac aga aga tac act act ttg atc ttg gac ttg ggt 48

Met Ala Ser Ile His Arg Arg Tyr Thr Thr Leu Ile Leu Asp Leu Gly

1 5 10 15

gac gtt ttg ttc aga tgg tct cca aag act gaa act gct atc cca cca 96

Asp Val Leu Phe Arg Trp Ser Pro Lys Thr Glu Thr Ala Ile Pro Pro

20 25 30

caa caa ttg aag gac atc ttg tct tct gtt act tgg ttc gaa tac gaa 144

Gln Gln Leu Lys Asp Ile Leu Ser Ser Val Thr Trp Phe Glu Tyr Glu

35 40 45

aga ggt aga ttg tct caa gaa gct tgt tac gaa aga tgt gct gaa gaa 192

Arg Gly Arg Leu Ser Gln Glu Ala Cys Tyr Glu Arg Cys Ala Glu Glu

50 55 60

ttc aag atc gaa gct tct gtt atc gct gaa gct ttc aag caa gct aga 240

Phe Lys Ile Glu Ala Ser Val Ile Ala Glu Ala Phe Lys Gln Ala Arg

65 70 75 80

ggt tct ttg aga cca aac gaa gaa ttc atc gct ttg atc aga gac ttg 288

Gly Ser Leu Arg Pro Asn Glu Glu Phe Ile Ala Leu Ile Arg Asp Leu

85 90 95

aga aga gaa atg cac ggt gac ttg act gtt ttg gct ttg tct aac atc 336

Arg Arg Glu Met His Gly Asp Leu Thr Val Leu Ala Leu Ser Asn Ile

100 105 110

tct ttg cca gac tac gaa tac atc atg tct ttg tct tct gac tgg act 384

Ser Leu Pro Asp Tyr Glu Tyr Ile Met Ser Leu Ser Ser Asp Trp Thr

115 120 125

act gtt ttc gac aga gtt ttc cca tct gct ttg gtt ggt gaa aga aag 432

Thr Val Phe Asp Arg Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys

130 135 140

cca cac ttg ggt tgt tac aga aag gtt atc tct gaa atg aac ttg gaa 480

Pro His Leu Gly Cys Tyr Arg Lys Val Ile Ser Glu Met Asn Leu Glu

145 150 155 160

cca caa act act gtt ttc gtt gac gac aag ttg gac aac gtt gct tct 528

Pro Gln Thr Thr Val Phe Val Asp Asp Lys Leu Asp Asn Val Ala Ser

165 170 175

gct aga tct ttg ggt atg cac ggt atc gtt ttc gac aac caa gct aac 576

Ala Arg Ser Leu Gly Met His Gly Ile Val Phe Asp Asn Gln Ala Asn

180 185 190

gtt ttc aga caa ttg aga aac atc ttc ggt gac cca atc aga aga ggt 624

Val Phe Arg Gln Leu Arg Asn Ile Phe Gly Asp Pro Ile Arg Arg Gly

195 200 205

caa gaa tac ttg aga ggt cac gct ggt aag ttg gaa tct tct act gac 672

Gln Glu Tyr Leu Arg Gly His Ala Gly Lys Leu Glu Ser Ser Thr Asp

210 215 220

aac ggt ttg atc ttc gaa gaa aac ttc act caa ttg atc atc tac gaa 720

Asn Gly Leu Ile Phe Glu Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu

225 230 235 240

ttg act caa gac aga act ttg atc tct ttg tct gaa tgt cca aga act 768

Leu Thr Gln Asp Arg Thr Leu Ile Ser Leu Ser Glu Cys Pro Arg Thr

245 250 255

tgg aac ttc ttc aga ggt gaa cca ttg ttc tct gaa act ttc cca gac 816

Trp Asn Phe Phe Arg Gly Glu Pro Leu Phe Ser Glu Thr Phe Pro Asp

260 265 270

gac gtt gac act act tct gtt gct ttg act gtt ttg caa cca gac aga 864

Asp Val Asp Thr Thr Ser Val Ala Leu Thr Val Leu Gln Pro Asp Arg

275 280 285

gct ttg gtt aac tct gtt ttg gac gaa atg ttg gaa tac gtt gac gct 912

Ala Leu Val Asn Ser Val Leu Asp Glu Met Leu Glu Tyr Val Asp Ala

290 295 300

gac ggt atc atg caa act tac ttc gac aga tct aga cca aga atg gac 960

Asp Gly Ile Met Gln Thr Tyr Phe Asp Arg Ser Arg Pro Arg Met Asp

305 310 315 320

cca ttc gtt tgt gtt aac gtt ttg tct ttg ttc tac gaa aac ggt aga 1008

Pro Phe Val Cys Val Asn Val Leu Ser Leu Phe Tyr Glu Asn Gly Arg

325 330 335

ggt cac gaa ttg cca aga act ttg gac tgg gtt tac gaa gtt ttg ttg 1056

Gly His Glu Leu Pro Arg Thr Leu Asp Trp Val Tyr Glu Val Leu Leu

340 345 350

cac aga gct tac cac ggt ggt tct aga tac tac ttg tct cca gac tgt 1104

His Arg Ala Tyr His Gly Gly Ser Arg Tyr Tyr Leu Ser Pro Asp Cys

355 360 365

ttc ttg ttc ttc atg tct aga ttg ttg aag aga gct gac gac cca gct 1152

Phe Leu Phe Phe Met Ser Arg Leu Leu Lys Arg Ala Asp Asp Pro Ala

370 375 380

gtt caa gct aga ttg aga cca ttg ttc gtt gaa aga gtt aac gaa aga 1200

Val Gln Ala Arg Leu Arg Pro Leu Phe Val Glu Arg Val Asn Glu Arg

385 390 395 400

gtt ggt gct gct ggt gac tct atg gac ttg gct ttc aga atc ttg gct 1248

Val Gly Ala Ala Gly Asp Ser Met Asp Leu Ala Phe Arg Ile Leu Ala

405 410 415

gct gct tct gtt ggt gtt caa tgt cca aga gac ttg gaa aga ttg act 1296

Ala Ala Ser Val Gly Val Gln Cys Pro Arg Asp Leu Glu Arg Leu Thr

420 425 430

gct ggt caa tgt gac gac ggt ggt tgg gac ttg tgt tgg ttc tac gtt 1344

Ala Gly Gln Cys Asp Asp Gly Gly Trp Asp Leu Cys Trp Phe Tyr Val

435 440 445

ttc ggt tct act ggt gtt aag gct ggt aac aga ggt ttg act act gct 1392

Phe Gly Ser Thr Gly Val Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala

450 455 460

ttg gct gtt act gct atc caa act gct atc ggt aga cca cca tct cca 1440

Leu Ala Val Thr Ala Ile Gln Thr Ala Ile Gly Arg Pro Pro Ser Pro

465 470 475 480

tct cca tct gct gct tct tct tct ttc aga cca tct tct cca tac aag 1488

Ser Pro Ser Ala Ala Ser Ser Ser Phe Arg Pro Ser Ser Pro Tyr Lys

485 490 495

ttc ttg ggt atc tct aga cca gct tct cca atc aga ttc ggt gac ttg 1536

Phe Leu Gly Ile Ser Arg Pro Ala Ser Pro Ile Arg Phe Gly Asp Leu

500 505 510

ttg aga cca tgg aga aag atg tct aga tct aac ttg aag tct caa taa 1584

Leu Arg Pro Trp Arg Lys Met Ser Arg Ser Asn Leu Lys Ser Gln

515 520 525

<210> 5

<211> 527

<212> PRT

<213> XP_007369631.1 污叉丝孔菌(Dichomitus squalens)折叶苔醇合酶，针对其在酿酒酵母中的表达经密码子优化

<400> 5

Met Ala Ser Ile His Arg Arg Tyr Thr Thr Leu Ile Leu Asp Leu Gly

1 5 10 15

Asp Val Leu Phe Arg Trp Ser Pro Lys Thr Glu Thr Ala Ile Pro Pro

20 25 30

Gln Gln Leu Lys Asp Ile Leu Ser Ser Val Thr Trp Phe Glu Tyr Glu

35 40 45

Arg Gly Arg Leu Ser Gln Glu Ala Cys Tyr Glu Arg Cys Ala Glu Glu

50 55 60

Phe Lys Ile Glu Ala Ser Val Ile Ala Glu Ala Phe Lys Gln Ala Arg

65 70 75 80

Gly Ser Leu Arg Pro Asn Glu Glu Phe Ile Ala Leu Ile Arg Asp Leu

85 90 95

Arg Arg Glu Met His Gly Asp Leu Thr Val Leu Ala Leu Ser Asn Ile

100 105 110

Ser Leu Pro Asp Tyr Glu Tyr Ile Met Ser Leu Ser Ser Asp Trp Thr

115 120 125

Thr Val Phe Asp Arg Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys

130 135 140

Pro His Leu Gly Cys Tyr Arg Lys Val Ile Ser Glu Met Asn Leu Glu

145 150 155 160

Pro Gln Thr Thr Val Phe Val Asp Asp Lys Leu Asp Asn Val Ala Ser

165 170 175

Ala Arg Ser Leu Gly Met His Gly Ile Val Phe Asp Asn Gln Ala Asn

180 185 190

Val Phe Arg Gln Leu Arg Asn Ile Phe Gly Asp Pro Ile Arg Arg Gly

195 200 205

Gln Glu Tyr Leu Arg Gly His Ala Gly Lys Leu Glu Ser Ser Thr Asp

210 215 220

Asn Gly Leu Ile Phe Glu Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu

225 230 235 240

Leu Thr Gln Asp Arg Thr Leu Ile Ser Leu Ser Glu Cys Pro Arg Thr

245 250 255

Trp Asn Phe Phe Arg Gly Glu Pro Leu Phe Ser Glu Thr Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Val Ala Leu Thr Val Leu Gln Pro Asp Arg

275 280 285

Ala Leu Val Asn Ser Val Leu Asp Glu Met Leu Glu Tyr Val Asp Ala

290 295 300

Asp Gly Ile Met Gln Thr Tyr Phe Asp Arg Ser Arg Pro Arg Met Asp

305 310 315 320

Pro Phe Val Cys Val Asn Val Leu Ser Leu Phe Tyr Glu Asn Gly Arg

325 330 335

Gly His Glu Leu Pro Arg Thr Leu Asp Trp Val Tyr Glu Val Leu Leu

340 345 350

His Arg Ala Tyr His Gly Gly Ser Arg Tyr Tyr Leu Ser Pro Asp Cys

355 360 365

Phe Leu Phe Phe Met Ser Arg Leu Leu Lys Arg Ala Asp Asp Pro Ala

370 375 380

Val Gln Ala Arg Leu Arg Pro Leu Phe Val Glu Arg Val Asn Glu Arg

385 390 395 400

Val Gly Ala Ala Gly Asp Ser Met Asp Leu Ala Phe Arg Ile Leu Ala

405 410 415

Ala Ala Ser Val Gly Val Gln Cys Pro Arg Asp Leu Glu Arg Leu Thr

420 425 430

Ala Gly Gln Cys Asp Asp Gly Gly Trp Asp Leu Cys Trp Phe Tyr Val

435 440 445

Phe Gly Ser Thr Gly Val Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala

450 455 460

Leu Ala Val Thr Ala Ile Gln Thr Ala Ile Gly Arg Pro Pro Ser Pro

465 470 475 480

Ser Pro Ser Ala Ala Ser Ser Ser Phe Arg Pro Ser Ser Pro Tyr Lys

485 490 495

Phe Leu Gly Ile Ser Arg Pro Ala Ser Pro Ile Arg Phe Gly Asp Leu

500 505 510

Leu Arg Pro Trp Arg Lys Met Ser Arg Ser Asn Leu Lys Ser Gln

515 520 525

<210> 6

<211> 1623

<212> DNA

<213> XP_006461126 双孢蘑菇(Agaricus bisporus)补身醇合酶序列，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1623)

<400> 6

atg gct cca cca caa aga cca ttc act gct atc gtt ttc gac atc ggt 48

Met Ala Pro Pro Gln Arg Pro Phe Thr Ala Ile Val Phe Asp Ile Gly

1 5 10 15

gac gtt ttg ttc caa tgg tct gct act act aag act tct atc tct cca 96

Asp Val Leu Phe Gln Trp Ser Ala Thr Thr Lys Thr Ser Ile Ser Pro

20 25 30

aag act ttg aga tct atc ttg aac tgt cca act tgg ttc gac tac gaa 144

Lys Thr Leu Arg Ser Ile Leu Asn Cys Pro Thr Trp Phe Asp Tyr Glu

35 40 45

aga ggt aga ttg gct gaa aac gct tgt tac gct gct atc tct caa gaa 192

Arg Gly Arg Leu Ala Glu Asn Ala Cys Tyr Ala Ala Ile Ser Gln Glu

50 55 60

ttc aac gtt aac cca gac gaa gtt aga gac gct ttc tct caa gct aga 240

Phe Asn Val Asn Pro Asp Glu Val Arg Asp Ala Phe Ser Gln Ala Arg

65 70 75 80

gac tct ttg caa gct aac cac gac ttc atc tct ttg atc aga gaa ttg 288

Asp Ser Leu Gln Ala Asn His Asp Phe Ile Ser Leu Ile Arg Glu Leu

85 90 95

aag gct caa gct aac ggt aga ttg aga gtt tac gct atg tct aac atc 336

Lys Ala Gln Ala Asn Gly Arg Leu Arg Val Tyr Ala Met Ser Asn Ile

100 105 110

tct ttg cca gac tgg gaa gtt ttg aga atg aag cca gct gac tgg gac 384

Ser Leu Pro Asp Trp Glu Val Leu Arg Met Lys Pro Ala Asp Trp Asp

115 120 125

atc ttc gac cac gtt ttc act tct ggt gct gtt ggt gaa aga aag cca 432

Ile Phe Asp His Val Phe Thr Ser Gly Ala Val Gly Glu Arg Lys Pro

130 135 140

aac ttg gct ttc tac aga cac gtt atc gct gct act gac ttg caa cca 480

Asn Leu Ala Phe Tyr Arg His Val Ile Ala Ala Thr Asp Leu Gln Pro

145 150 155 160

cac caa act atc ttc gtt gac gac aag ttg gaa aac gtt ttg tct gct 528

His Gln Thr Ile Phe Val Asp Asp Lys Leu Glu Asn Val Leu Ser Ala

165 170 175

aga tct ttg ggt ttc act ggt atc gtt ttc gac gaa cca tct gaa gtt 576

Arg Ser Leu Gly Phe Thr Gly Ile Val Phe Asp Glu Pro Ser Glu Val

180 185 190

aag aga gct ttg aga aac ttg atc ggt gac cca gtt caa aga ggt ggt 624

Lys Arg Ala Leu Arg Asn Leu Ile Gly Asp Pro Val Gln Arg Gly Gly

195 200 205

gaa ttc ttg gtt aga aac gct ggt aag ttg ggt tct atc act aga act 672

Glu Phe Leu Val Arg Asn Ala Gly Lys Leu Gly Ser Ile Thr Arg Thr

210 215 220

act gct aag cac gaa tct atc cca ttg gac gaa aac ttc gct caa ttg 720

Thr Ala Lys His Glu Ser Ile Pro Leu Asp Glu Asn Phe Ala Gln Leu

225 230 235 240

ttg atc ttg gaa atc act ggt aac aga gct ttg gtt aac ttg gtt gaa 768

Leu Ile Leu Glu Ile Thr Gly Asn Arg Ala Leu Val Asn Leu Val Glu

245 250 255

cac cca caa act tgg aac ttc ttc caa ggt aag ggt caa ttg act act 816

His Pro Gln Thr Trp Asn Phe Phe Gln Gly Lys Gly Gln Leu Thr Thr

260 265 270

gaa gaa ttc cca ttc gac ttg gac act act tct ttg ggt ttg act atc 864

Glu Glu Phe Pro Phe Asp Leu Asp Thr Thr Ser Leu Gly Leu Thr Ile

275 280 285

ttg aag aga tct aga gaa atc gct gac tct gtt atg gac gaa atg ttg 912

Leu Lys Arg Ser Arg Glu Ile Ala Asp Ser Val Met Asp Glu Met Leu

290 295 300

gaa tac gtt gac cca gac ggt atc atc caa act tac ttc gac cac aga 960

Glu Tyr Val Asp Pro Asp Gly Ile Ile Gln Thr Tyr Phe Asp His Arg

305 310 315 320

aga cca aga ttc gac cca gtt gtt tgt gtt aac gct ttg tct ttg ttc 1008

Arg Pro Arg Phe Asp Pro Val Val Cys Val Asn Ala Leu Ser Leu Phe

325 330 335

tac gct tac ggt aga ggt gaa caa ttg aga tct act ttg act tgg gtt 1056

Tyr Ala Tyr Gly Arg Gly Glu Gln Leu Arg Ser Thr Leu Thr Trp Val

340 345 350

cac gaa gtt ttg ttg aac aga gct tac ttg gac ggt act aga tac tac 1104

His Glu Val Leu Leu Asn Arg Ala Tyr Leu Asp Gly Thr Arg Tyr Tyr

355 360 365

gaa act gct gaa tgt ttc ttg tac ttc atg tct aga ttg ttg gct act 1152

Glu Thr Ala Glu Cys Phe Leu Tyr Phe Met Ser Arg Leu Leu Ala Thr

370 375 380

tct ggt gac cca gac ttg cac tct ttg ttg aag cca ttg ttg aag gaa 1200

Ser Gly Asp Pro Asp Leu His Ser Leu Leu Lys Pro Leu Leu Lys Glu

385 390 395 400

aga gtt caa gaa aga atc ggt gct gac ggt gac tct ttg gct ttg gct 1248

Arg Val Gln Glu Arg Ile Gly Ala Asp Gly Asp Ser Leu Ala Leu Ala

405 410 415

atg aga atc ttg gct tgt gac ttc gtt ggt atc aga gac gaa gtt gac 1296

Met Arg Ile Leu Ala Cys Asp Phe Val Gly Ile Arg Asp Glu Val Asp

420 425 430

ttg aga act ttg ttg act ttg caa tgt gaa gac ggt ggt tgg gaa gtt 1344

Leu Arg Thr Leu Leu Thr Leu Gln Cys Glu Asp Gly Gly Trp Glu Val

435 440 445

ggt tgg atg tac aag tac ggt tct tct ggt atc tct atc ggt aac aga 1392

Gly Trp Met Tyr Lys Tyr Gly Ser Ser Gly Ile Ser Ile Gly Asn Arg

450 455 460

ggt ttg gct act gct ttg gct atc aag gct gtt gac act atg ttc caa 1440

Gly Leu Ala Thr Ala Leu Ala Ile Lys Ala Val Asp Thr Met Phe Gln

465 470 475 480

cca caa atc aga ttc tct gaa tct cca act gac act ttg gtt gaa aac 1488

Pro Gln Ile Arg Phe Ser Glu Ser Pro Thr Asp Thr Leu Val Glu Asn

485 490 495

gct atc cac aag aga aga cca tct ttc tct gaa aag ttc ttg ggt aag 1536

Ala Ile His Lys Arg Arg Pro Ser Phe Ser Glu Lys Phe Leu Gly Lys

500 505 510

aga cca aga tct ggt tct ttc aga aag cca ttg caa tgg atc ttg caa 1584

Arg Pro Arg Ser Gly Ser Phe Arg Lys Pro Leu Gln Trp Ile Leu Gln

515 520 525

ggt tct aag ttg aga aag tct gtt gaa atc ggt tct taa 1623

Gly Ser Lys Leu Arg Lys Ser Val Glu Ile Gly Ser

530 535 540

<210> 7

<211> 540

<212> PRT

<213> XP_006461126 双孢蘑菇(Agaricus bisporus)补身醇合酶序列，针对其在酿酒酵母中的表达经密码子优化

<400> 7

Met Ala Pro Pro Gln Arg Pro Phe Thr Ala Ile Val Phe Asp Ile Gly

1 5 10 15

Asp Val Leu Phe Gln Trp Ser Ala Thr Thr Lys Thr Ser Ile Ser Pro

20 25 30

Lys Thr Leu Arg Ser Ile Leu Asn Cys Pro Thr Trp Phe Asp Tyr Glu

35 40 45

Arg Gly Arg Leu Ala Glu Asn Ala Cys Tyr Ala Ala Ile Ser Gln Glu

50 55 60

Phe Asn Val Asn Pro Asp Glu Val Arg Asp Ala Phe Ser Gln Ala Arg

65 70 75 80

Asp Ser Leu Gln Ala Asn His Asp Phe Ile Ser Leu Ile Arg Glu Leu

85 90 95

Lys Ala Gln Ala Asn Gly Arg Leu Arg Val Tyr Ala Met Ser Asn Ile

100 105 110

Ser Leu Pro Asp Trp Glu Val Leu Arg Met Lys Pro Ala Asp Trp Asp

115 120 125

Ile Phe Asp His Val Phe Thr Ser Gly Ala Val Gly Glu Arg Lys Pro

130 135 140

Asn Leu Ala Phe Tyr Arg His Val Ile Ala Ala Thr Asp Leu Gln Pro

145 150 155 160

His Gln Thr Ile Phe Val Asp Asp Lys Leu Glu Asn Val Leu Ser Ala

165 170 175

Arg Ser Leu Gly Phe Thr Gly Ile Val Phe Asp Glu Pro Ser Glu Val

180 185 190

Lys Arg Ala Leu Arg Asn Leu Ile Gly Asp Pro Val Gln Arg Gly Gly

195 200 205

Glu Phe Leu Val Arg Asn Ala Gly Lys Leu Gly Ser Ile Thr Arg Thr

210 215 220

Thr Ala Lys His Glu Ser Ile Pro Leu Asp Glu Asn Phe Ala Gln Leu

225 230 235 240

Leu Ile Leu Glu Ile Thr Gly Asn Arg Ala Leu Val Asn Leu Val Glu

245 250 255

His Pro Gln Thr Trp Asn Phe Phe Gln Gly Lys Gly Gln Leu Thr Thr

260 265 270

Glu Glu Phe Pro Phe Asp Leu Asp Thr Thr Ser Leu Gly Leu Thr Ile

275 280 285

Leu Lys Arg Ser Arg Glu Ile Ala Asp Ser Val Met Asp Glu Met Leu

290 295 300

Glu Tyr Val Asp Pro Asp Gly Ile Ile Gln Thr Tyr Phe Asp His Arg

305 310 315 320

Arg Pro Arg Phe Asp Pro Val Val Cys Val Asn Ala Leu Ser Leu Phe

325 330 335

Tyr Ala Tyr Gly Arg Gly Glu Gln Leu Arg Ser Thr Leu Thr Trp Val

340 345 350

His Glu Val Leu Leu Asn Arg Ala Tyr Leu Asp Gly Thr Arg Tyr Tyr

355 360 365

Glu Thr Ala Glu Cys Phe Leu Tyr Phe Met Ser Arg Leu Leu Ala Thr

370 375 380

Ser Gly Asp Pro Asp Leu His Ser Leu Leu Lys Pro Leu Leu Lys Glu

385 390 395 400

Arg Val Gln Glu Arg Ile Gly Ala Asp Gly Asp Ser Leu Ala Leu Ala

405 410 415

Met Arg Ile Leu Ala Cys Asp Phe Val Gly Ile Arg Asp Glu Val Asp

420 425 430

Leu Arg Thr Leu Leu Thr Leu Gln Cys Glu Asp Gly Gly Trp Glu Val

435 440 445

Gly Trp Met Tyr Lys Tyr Gly Ser Ser Gly Ile Ser Ile Gly Asn Arg

450 455 460

Gly Leu Ala Thr Ala Leu Ala Ile Lys Ala Val Asp Thr Met Phe Gln

465 470 475 480

Pro Gln Ile Arg Phe Ser Glu Ser Pro Thr Asp Thr Leu Val Glu Asn

485 490 495

Ala Ile His Lys Arg Arg Pro Ser Phe Ser Glu Lys Phe Leu Gly Lys

500 505 510

Arg Pro Arg Ser Gly Ser Phe Arg Lys Pro Leu Gln Trp Ile Leu Gln

515 520 525

Gly Ser Lys Leu Arg Lys Ser Val Glu Ile Gly Ser

530 535 540

<210> 8

<211> 1320

<212> DNA

<213> CrDAT，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1320)

<400> 8

atg gaa agt ggt aaa ata tct gta gaa acg gaa acc cta tcg aaa acc 48

Met Glu Ser Gly Lys Ile Ser Val Glu Thr Glu Thr Leu Ser Lys Thr

1 5 10 15

ctt atc aag ccg tcc tcg ccc acg cca cag tct ctt tcc cgt tac aac 96

Leu Ile Lys Pro Ser Ser Pro Thr Pro Gln Ser Leu Ser Arg Tyr Asn

20 25 30

tta tct tac aat gat cag aac att tat cag acc tgc gta tct gta ggg 144

Leu Ser Tyr Asn Asp Gln Asn Ile Tyr Gln Thr Cys Val Ser Val Gly

35 40 45

ttc ttt tac gaa aat cct gat ggc atc gag ata tcc acg atc cgt gag 192

Phe Phe Tyr Glu Asn Pro Asp Gly Ile Glu Ile Ser Thr Ile Arg Glu

50 55 60

cag ctg cag aac agc ctg tcc aaa act ctg gtc tca tac tat ccc ttc 240

Gln Leu Gln Asn Ser Leu Ser Lys Thr Leu Val Ser Tyr Tyr Pro Phe

65 70 75 80

gca ggc aaa gta gtg aag aac gac tac ata cat tgt aac gac gat ggc 288

Ala Gly Lys Val Val Lys Asn Asp Tyr Ile His Cys Asn Asp Asp Gly

85 90 95

att gag ttc gtc gaa gtc aga att aga tgc agg atg aat gat ata ctt 336

Ile Glu Phe Val Glu Val Arg Ile Arg Cys Arg Met Asn Asp Ile Leu

100 105 110

aaa tac gaa cta cgt tct tac gct cgt gat tta gtc ctg cca aag agg 384

Lys Tyr Glu Leu Arg Ser Tyr Ala Arg Asp Leu Val Leu Pro Lys Arg

115 120 125

gtt acc gtg ggc tct gaa gat acc acc gct atc gtg cag ctg tcc cat 432

Val Thr Val Gly Ser Glu Asp Thr Thr Ala Ile Val Gln Leu Ser His

130 135 140

ttc gac tgt ggc ggt ttg gcc gtg gct ttt ggt atc tcc cac aag gtg 480

Phe Asp Cys Gly Gly Leu Ala Val Ala Phe Gly Ile Ser His Lys Val

145 150 155 160

gcc gat ggt ggt act ata gcc tct ttc atg aag gac tgg gcg gcc tct 528

Ala Asp Gly Gly Thr Ile Ala Ser Phe Met Lys Asp Trp Ala Ala Ser

165 170 175

gct tgc tac ttg tcc tca agt cac cac gtt cca acg cct cta ttg gtc 576

Ala Cys Tyr Leu Ser Ser Ser His His Val Pro Thr Pro Leu Leu Val

180 185 190

tca gac agt att ttc cca aga cag gac aac ata ata tgc gaa caa ttt 624

Ser Asp Ser Ile Phe Pro Arg Gln Asp Asn Ile Ile Cys Glu Gln Phe

195 200 205

ccc acc tca aag aat tgc gtg gag aag aca ttc att ttc cca cca gaa 672

Pro Thr Ser Lys Asn Cys Val Glu Lys Thr Phe Ile Phe Pro Pro Glu

210 215 220

gca atc gaa aag tta aag agt aag gcc gtt gaa ttc ggc att gag aaa 720

Ala Ile Glu Lys Leu Lys Ser Lys Ala Val Glu Phe Gly Ile Glu Lys

225 230 235 240

ccg acc aga gta gag gtc ctg act gca ttc tta tct aga tgc gcc acc 768

Pro Thr Arg Val Glu Val Leu Thr Ala Phe Leu Ser Arg Cys Ala Thr

245 250 255

gta gca ggt aag tcg gca gct aag aac aac aat tgt ggt caa agc ctg 816

Val Ala Gly Lys Ser Ala Ala Lys Asn Asn Asn Cys Gly Gln Ser Leu

260 265 270

ccc ttt ccg gtt cta cag gcc att aat ttg agg ccg att cta gaa ttg 864

Pro Phe Pro Val Leu Gln Ala Ile Asn Leu Arg Pro Ile Leu Glu Leu

275 280 285

cca cag aac tct gtg ggt aat cta gtt tcg atc tac ttc agc agg aca 912

Pro Gln Asn Ser Val Gly Asn Leu Val Ser Ile Tyr Phe Ser Arg Thr

290 295 300

att aag gaa aac gac tac cta aat gaa aag gag tac act aaa ttg gtg 960

Ile Lys Glu Asn Asp Tyr Leu Asn Glu Lys Glu Tyr Thr Lys Leu Val

305 310 315 320

ata aac gag ttg cgt aaa gaa aag caa aag atc aag aat ttg agc aga 1008

Ile Asn Glu Leu Arg Lys Glu Lys Gln Lys Ile Lys Asn Leu Ser Arg

325 330 335

gag aag ttg acc tac gtc gcc cag atg gaa gag ttc gtt aag tca ctt 1056

Glu Lys Leu Thr Tyr Val Ala Gln Met Glu Glu Phe Val Lys Ser Leu

340 345 350

aaa gag ttc gat atc tcc aac ttc cta gat atc gat gcc tac tta agc 1104

Lys Glu Phe Asp Ile Ser Asn Phe Leu Asp Ile Asp Ala Tyr Leu Ser

355 360 365

gat agt tgg tgc agg ttt cct ttc tac gac gtc gac ttc gga tgg gga 1152

Asp Ser Trp Cys Arg Phe Pro Phe Tyr Asp Val Asp Phe Gly Trp Gly

370 375 380

aag cct atc tgg gtc tgc cta ttc cag cct tac atc aag aac tgc gtt 1200

Lys Pro Ile Trp Val Cys Leu Phe Gln Pro Tyr Ile Lys Asn Cys Val

385 390 395 400

gtt atg atg gac tat cct ttc ggt gac gat tat ggt atc gag gct att 1248

Val Met Met Asp Tyr Pro Phe Gly Asp Asp Tyr Gly Ile Glu Ala Ile

405 410 415

gtt tct ttc gag caa gag aaa atg tcc gcg ttc gag aag aac gaa cag 1296

Val Ser Phe Glu Gln Glu Lys Met Ser Ala Phe Glu Lys Asn Glu Gln

420 425 430

tta ctg cag ttt gtg tct aac taa 1320

Leu Leu Gln Phe Val Ser Asn

435

<210> 9

<211> 439

<212> PRT

<213> CrDAT，针对其在酿酒酵母中的表达经密码子优化

<400> 9

Met Glu Ser Gly Lys Ile Ser Val Glu Thr Glu Thr Leu Ser Lys Thr

1 5 10 15

Leu Ile Lys Pro Ser Ser Pro Thr Pro Gln Ser Leu Ser Arg Tyr Asn

20 25 30

Leu Ser Tyr Asn Asp Gln Asn Ile Tyr Gln Thr Cys Val Ser Val Gly

35 40 45

Phe Phe Tyr Glu Asn Pro Asp Gly Ile Glu Ile Ser Thr Ile Arg Glu

50 55 60

Gln Leu Gln Asn Ser Leu Ser Lys Thr Leu Val Ser Tyr Tyr Pro Phe

65 70 75 80

Ala Gly Lys Val Val Lys Asn Asp Tyr Ile His Cys Asn Asp Asp Gly

85 90 95

Ile Glu Phe Val Glu Val Arg Ile Arg Cys Arg Met Asn Asp Ile Leu

100 105 110

Lys Tyr Glu Leu Arg Ser Tyr Ala Arg Asp Leu Val Leu Pro Lys Arg

115 120 125

Val Thr Val Gly Ser Glu Asp Thr Thr Ala Ile Val Gln Leu Ser His

130 135 140

Phe Asp Cys Gly Gly Leu Ala Val Ala Phe Gly Ile Ser His Lys Val

145 150 155 160

Ala Asp Gly Gly Thr Ile Ala Ser Phe Met Lys Asp Trp Ala Ala Ser

165 170 175

Ala Cys Tyr Leu Ser Ser Ser His His Val Pro Thr Pro Leu Leu Val

180 185 190

Ser Asp Ser Ile Phe Pro Arg Gln Asp Asn Ile Ile Cys Glu Gln Phe

195 200 205

Pro Thr Ser Lys Asn Cys Val Glu Lys Thr Phe Ile Phe Pro Pro Glu

210 215 220

Ala Ile Glu Lys Leu Lys Ser Lys Ala Val Glu Phe Gly Ile Glu Lys

225 230 235 240

Pro Thr Arg Val Glu Val Leu Thr Ala Phe Leu Ser Arg Cys Ala Thr

245 250 255

Val Ala Gly Lys Ser Ala Ala Lys Asn Asn Asn Cys Gly Gln Ser Leu

260 265 270

Pro Phe Pro Val Leu Gln Ala Ile Asn Leu Arg Pro Ile Leu Glu Leu

275 280 285

Pro Gln Asn Ser Val Gly Asn Leu Val Ser Ile Tyr Phe Ser Arg Thr

290 295 300

Ile Lys Glu Asn Asp Tyr Leu Asn Glu Lys Glu Tyr Thr Lys Leu Val

305 310 315 320

Ile Asn Glu Leu Arg Lys Glu Lys Gln Lys Ile Lys Asn Leu Ser Arg

325 330 335

Glu Lys Leu Thr Tyr Val Ala Gln Met Glu Glu Phe Val Lys Ser Leu

340 345 350

Lys Glu Phe Asp Ile Ser Asn Phe Leu Asp Ile Asp Ala Tyr Leu Ser

355 360 365

Asp Ser Trp Cys Arg Phe Pro Phe Tyr Asp Val Asp Phe Gly Trp Gly

370 375 380

Lys Pro Ile Trp Val Cys Leu Phe Gln Pro Tyr Ile Lys Asn Cys Val

385 390 395 400

Val Met Met Asp Tyr Pro Phe Gly Asp Asp Tyr Gly Ile Glu Ala Ile

405 410 415

Val Ser Phe Glu Gln Glu Lys Met Ser Ala Phe Glu Lys Asn Glu Gln

420 425 430

Leu Leu Gln Phe Val Ser Asn

435

<210> 10

<211> 1320

<212> DNA

<213> TcTAT，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1320)

<400> 10

atg gag aag acg gat tta cac gtg aac ctt atc gaa aag gtt atg gtc 48

Met Glu Lys Thr Asp Leu His Val Asn Leu Ile Glu Lys Val Met Val

1 5 10 15

ggt cca agc cca cct ttg ccg aag act aca cta caa cta tcc tca atc 96

Gly Pro Ser Pro Pro Leu Pro Lys Thr Thr Leu Gln Leu Ser Ser Ile

20 25 30

gat aac cta cct ggt gtt aga ggc tcg atc ttt aat gcc ttg tta ata 144

Asp Asn Leu Pro Gly Val Arg Gly Ser Ile Phe Asn Ala Leu Leu Ile

35 40 45

tat aat gct tca ccg tct ccc acg atg atc tcc gct gat cca gct aaa 192

Tyr Asn Ala Ser Pro Ser Pro Thr Met Ile Ser Ala Asp Pro Ala Lys

50 55 60

cct atc aga gaa gct ttg gcc aaa atc ctg gtt tac tac cca cct ttc 240

Pro Ile Arg Glu Ala Leu Ala Lys Ile Leu Val Tyr Tyr Pro Pro Phe

65 70 75 80

gct ggc agg cta cgt gag act gag aat ggc gat cta gag gta gag tgc 288

Ala Gly Arg Leu Arg Glu Thr Glu Asn Gly Asp Leu Glu Val Glu Cys

85 90 95

acg gga gag ggt gcg atg ttt ctt gaa gcg atg gcc gat aat gaa tta 336

Thr Gly Glu Gly Ala Met Phe Leu Glu Ala Met Ala Asp Asn Glu Leu

100 105 110

agt gtc tta ggg gat ttc gac gac agt aac cct agc ttc cag caa ttg 384

Ser Val Leu Gly Asp Phe Asp Asp Ser Asn Pro Ser Phe Gln Gln Leu

115 120 125

ttg ttc tcc ctt cca ctg gac act aac ttc aag gat cta tcc ctg tta 432

Leu Phe Ser Leu Pro Leu Asp Thr Asn Phe Lys Asp Leu Ser Leu Leu

130 135 140

gtc gtg cag gtg aca agg ttt aca tgc ggc ggg ttc gtc gtt ggg gtt 480

Val Val Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val

145 150 155 160

tca ttt cac cac ggt gta tgc gat ggg aga gga gct gca caa ttt ctg 528

Ser Phe His His Gly Val Cys Asp Gly Arg Gly Ala Ala Gln Phe Leu

165 170 175

aag gga cta gcc gaa atg gca agg gga gaa gtt aag ttg tcg ctg gag 576

Lys Gly Leu Ala Glu Met Ala Arg Gly Glu Val Lys Leu Ser Leu Glu

180 185 190

ccg atc tgg aat cgt gaa cta gtt aag ctg gac gat ccc aag tat ttg 624

Pro Ile Trp Asn Arg Glu Leu Val Lys Leu Asp Asp Pro Lys Tyr Leu

195 200 205

caa ttc ttt cac ttc gaa ttc ttg aga gca cca tca atc gtc gaa aag 672

Gln Phe Phe His Phe Glu Phe Leu Arg Ala Pro Ser Ile Val Glu Lys

210 215 220

ata gtt caa acc tac ttc atc atc gat ttc gaa act att aac tac ata 720

Ile Val Gln Thr Tyr Phe Ile Ile Asp Phe Glu Thr Ile Asn Tyr Ile

225 230 235 240

aag cag tca gta atg gaa gaa tgt aaa gaa ttc tgc agc tct ttt gaa 768

Lys Gln Ser Val Met Glu Glu Cys Lys Glu Phe Cys Ser Ser Phe Glu

245 250 255

gtt gct tct gca atg acg tgg ata gct agg acc agg gcc ttt caa ata 816

Val Ala Ser Ala Met Thr Trp Ile Ala Arg Thr Arg Ala Phe Gln Ile

260 265 270

ccc gag agc gag tac gtg aaa atc cta ttt ggt atg gac atg agg aac 864

Pro Glu Ser Glu Tyr Val Lys Ile Leu Phe Gly Met Asp Met Arg Asn

275 280 285

tct ttt aac ccg cct ctg ccg tca ggt tat tat gga aac tca att ggt 912

Ser Phe Asn Pro Pro Leu Pro Ser Gly Tyr Tyr Gly Asn Ser Ile Gly

290 295 300

acg gct tgt gca gtg gac aat gtt caa gac ttg ttg agt ggt tca cta 960

Thr Ala Cys Ala Val Asp Asn Val Gln Asp Leu Leu Ser Gly Ser Leu

305 310 315 320

ctg agg gca ata atg ata atc aag aaa tca aag gta tca ctt aac gac 1008

Leu Arg Ala Ile Met Ile Ile Lys Lys Ser Lys Val Ser Leu Asn Asp

325 330 335

aac ttt aag agc cgt gct gtc gtt aag cct tca gaa ctt gac gta aac 1056

Asn Phe Lys Ser Arg Ala Val Val Lys Pro Ser Glu Leu Asp Val Asn

340 345 350

atg aac cat gag aat gtg gtg gca ttt gct gac tgg tca agg ctg ggg 1104

Met Asn His Glu Asn Val Val Ala Phe Ala Asp Trp Ser Arg Leu Gly

355 360 365

ttc gac gaa gtc gat ttt ggc tgg gga aac gcg gtg tct gtt tcc ccg 1152

Phe Asp Glu Val Asp Phe Gly Trp Gly Asn Ala Val Ser Val Ser Pro

370 375 380

gta cag caa cag tcg gca ctt gca atg cag aat tac ttc ctg ttc ttg 1200

Val Gln Gln Gln Ser Ala Leu Ala Met Gln Asn Tyr Phe Leu Phe Leu

385 390 395 400

aag cca agc aag aac aag ccc gat gga att aag att cta atg ttc cta 1248

Lys Pro Ser Lys Asn Lys Pro Asp Gly Ile Lys Ile Leu Met Phe Leu

405 410 415

cca ttg tcc aag atg aaa tcc ttc aaa att gaa atg gaa gcg atg atg 1296

Pro Leu Ser Lys Met Lys Ser Phe Lys Ile Glu Met Glu Ala Met Met

420 425 430

aag aag tac gtc gcc aag gta taa 1320

Lys Lys Tyr Val Ala Lys Val

435

<210> 11

<211> 439

<212> PRT

<213> TcTAT，针对其在酿酒酵母中的表达经密码子优化

<400> 11

Met Glu Lys Thr Asp Leu His Val Asn Leu Ile Glu Lys Val Met Val

1 5 10 15

Gly Pro Ser Pro Pro Leu Pro Lys Thr Thr Leu Gln Leu Ser Ser Ile

20 25 30

Asp Asn Leu Pro Gly Val Arg Gly Ser Ile Phe Asn Ala Leu Leu Ile

35 40 45

Tyr Asn Ala Ser Pro Ser Pro Thr Met Ile Ser Ala Asp Pro Ala Lys

50 55 60

Pro Ile Arg Glu Ala Leu Ala Lys Ile Leu Val Tyr Tyr Pro Pro Phe

65 70 75 80

Ala Gly Arg Leu Arg Glu Thr Glu Asn Gly Asp Leu Glu Val Glu Cys

85 90 95

Thr Gly Glu Gly Ala Met Phe Leu Glu Ala Met Ala Asp Asn Glu Leu

100 105 110

Ser Val Leu Gly Asp Phe Asp Asp Ser Asn Pro Ser Phe Gln Gln Leu

115 120 125

Leu Phe Ser Leu Pro Leu Asp Thr Asn Phe Lys Asp Leu Ser Leu Leu

130 135 140

Val Val Gln Val Thr Arg Phe Thr Cys Gly Gly Phe Val Val Gly Val

145 150 155 160

Ser Phe His His Gly Val Cys Asp Gly Arg Gly Ala Ala Gln Phe Leu

165 170 175

Lys Gly Leu Ala Glu Met Ala Arg Gly Glu Val Lys Leu Ser Leu Glu

180 185 190

Pro Ile Trp Asn Arg Glu Leu Val Lys Leu Asp Asp Pro Lys Tyr Leu

195 200 205

Gln Phe Phe His Phe Glu Phe Leu Arg Ala Pro Ser Ile Val Glu Lys

210 215 220

Ile Val Gln Thr Tyr Phe Ile Ile Asp Phe Glu Thr Ile Asn Tyr Ile

225 230 235 240

Lys Gln Ser Val Met Glu Glu Cys Lys Glu Phe Cys Ser Ser Phe Glu

245 250 255

Val Ala Ser Ala Met Thr Trp Ile Ala Arg Thr Arg Ala Phe Gln Ile

260 265 270

Pro Glu Ser Glu Tyr Val Lys Ile Leu Phe Gly Met Asp Met Arg Asn

275 280 285

Ser Phe Asn Pro Pro Leu Pro Ser Gly Tyr Tyr Gly Asn Ser Ile Gly

290 295 300

Thr Ala Cys Ala Val Asp Asn Val Gln Asp Leu Leu Ser Gly Ser Leu

305 310 315 320

Leu Arg Ala Ile Met Ile Ile Lys Lys Ser Lys Val Ser Leu Asn Asp

325 330 335

Asn Phe Lys Ser Arg Ala Val Val Lys Pro Ser Glu Leu Asp Val Asn

340 345 350

Met Asn His Glu Asn Val Val Ala Phe Ala Asp Trp Ser Arg Leu Gly

355 360 365

Phe Asp Glu Val Asp Phe Gly Trp Gly Asn Ala Val Ser Val Ser Pro

370 375 380

Val Gln Gln Gln Ser Ala Leu Ala Met Gln Asn Tyr Phe Leu Phe Leu

385 390 395 400

Lys Pro Ser Lys Asn Lys Pro Asp Gly Ile Lys Ile Leu Met Phe Leu

405 410 415

Pro Leu Ser Lys Met Lys Ser Phe Lys Ile Glu Met Glu Ala Met Met

420 425 430

Lys Lys Tyr Val Ala Lys Val

435

<210> 12

<211> 1332

<212> DNA

<213> CrMAT，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1332)

<400> 12

atg gat tct att act atg gtt gaa acc gag aca tta tcg aag aca ttg 48

Met Asp Ser Ile Thr Met Val Glu Thr Glu Thr Leu Ser Lys Thr Leu

1 5 10 15

att aag cca tct agc cca acc cca cag agt ctg tca cac tac aac ctg 96

Ile Lys Pro Ser Ser Pro Thr Pro Gln Ser Leu Ser His Tyr Asn Leu

20 25 30

tct tac aac gat cag aat ata tat cca gag tat ata ttt gca ggc ttc 144

Ser Tyr Asn Asp Gln Asn Ile Tyr Pro Glu Tyr Ile Phe Ala Gly Phe

35 40 45

ttc tac agc aac ccg gac gga cac gag atc tcg act att aga gag cag 192

Phe Tyr Ser Asn Pro Asp Gly His Glu Ile Ser Thr Ile Arg Glu Gln

50 55 60

ctg cag aac tct ctt agc aag aca tta gta tct tat tat cca ttc gct 240

Leu Gln Asn Ser Leu Ser Lys Thr Leu Val Ser Tyr Tyr Pro Phe Ala

65 70 75 80

ggt aaa gtg gtc aag aac gat tac att cac tgc aat gac gac gga ata 288

Gly Lys Val Val Lys Asn Asp Tyr Ile His Cys Asn Asp Asp Gly Ile

85 90 95

gaa ttc gta gac gtc aga atc cac tgc agg atg aac gat att ctt aag 336

Glu Phe Val Asp Val Arg Ile His Cys Arg Met Asn Asp Ile Leu Lys

100 105 110

cct gaa ttg agg tct tac gct tcc gag ctt att cgt ccg aat aga agt 384

Pro Glu Leu Arg Ser Tyr Ala Ser Glu Leu Ile Arg Pro Asn Arg Ser

115 120 125

aca gtg ggg tca gag gac agt act gcg ctt gtc cag tta tct cac ttt 432

Thr Val Gly Ser Glu Asp Ser Thr Ala Leu Val Gln Leu Ser His Phe

130 135 140

gac tgt ggc ggg gta gct gtc gca ttc ggt ata tct cac aaa gtc gca 480

Asp Cys Gly Gly Val Ala Val Ala Phe Gly Ile Ser His Lys Val Ala

145 150 155 160

gac gca gcg act att ctg tcg ttt ata aag gat tgg gcc gct tct acg 528

Asp Ala Ala Thr Ile Leu Ser Phe Ile Lys Asp Trp Ala Ala Ser Thr

165 170 175

tgt gac ttg tca agt agt cac gat gtg tct aca cca gta cta gta tcc 576

Cys Asp Leu Ser Ser Ser His Asp Val Ser Thr Pro Val Leu Val Ser

180 185 190

gat tcc ata ttc ccc cgt caa gat aac atc atc tgt ggc cag ttc cct 624

Asp Ser Ile Phe Pro Arg Gln Asp Asn Ile Ile Cys Gly Gln Phe Pro

195 200 205

gct tca ccg aac tgc gtg agg aag cgt ttc tta ttc agc ccg gaa gct 672

Ala Ser Pro Asn Cys Val Arg Lys Arg Phe Leu Phe Ser Pro Glu Ala

210 215 220

atc gaa aga cta aaa tcg aaa gcc att gaa ttt ggg atc gag aag cca 720

Ile Glu Arg Leu Lys Ser Lys Ala Ile Glu Phe Gly Ile Glu Lys Pro

225 230 235 240

acg agg gta gag gtc ctg aca gca ttc ttg tgc cgt tgc gct acc gtt 768

Thr Arg Val Glu Val Leu Thr Ala Phe Leu Cys Arg Cys Ala Thr Val

245 250 255

gca ggt aaa tct gcg gcc aag aac aac aat tgt gga cag tca ctg cct 816

Ala Gly Lys Ser Ala Ala Lys Asn Asn Asn Cys Gly Gln Ser Leu Pro

260 265 270

ttc gct gtt att caa gca gtc aac ctg agg ccc tta cta gaa ctg cca 864

Phe Ala Val Ile Gln Ala Val Asn Leu Arg Pro Leu Leu Glu Leu Pro

275 280 285

aag aat tcc gtc ggc aac ctt ata tca atc tac ttt tct aca atc aaa 912

Lys Asn Ser Val Gly Asn Leu Ile Ser Ile Tyr Phe Ser Thr Ile Lys

290 295 300

gaa aac gac acg gtg aac atc gaa cag gag ttc aca aaa tta gtg atc 960

Glu Asn Asp Thr Val Asn Ile Glu Gln Glu Phe Thr Lys Leu Val Ile

305 310 315 320

gga gag ttg agg aag gct aag gat aag tta aag aac ctg tcg caa gag 1008

Gly Glu Leu Arg Lys Ala Lys Asp Lys Leu Lys Asn Leu Ser Gln Glu

325 330 335

aag ctg aat tac gta gct aga atg caa gat ttc gcg aat tgc ctg aag 1056

Lys Leu Asn Tyr Val Ala Arg Met Gln Asp Phe Ala Asn Cys Leu Lys

340 345 350

gaa ttg gac ata agt tca ttc ttc gac atg gaa aac gtg gac ata gac 1104

Glu Leu Asp Ile Ser Ser Phe Phe Asp Met Glu Asn Val Asp Ile Asp

355 360 365

gct tat tta ttt tcg agc tgg tgc agg ttc ccc ttc tac gac atc gat 1152

Ala Tyr Leu Phe Ser Ser Trp Cys Arg Phe Pro Phe Tyr Asp Ile Asp

370 375 380

ttc ggt ctg ggg aag cca ata tgg gtc tgc atg ttt cag cct cac ttt 1200

Phe Gly Leu Gly Lys Pro Ile Trp Val Cys Met Phe Gln Pro His Phe

385 390 395 400

aag aat tgt ata att tta atg gat tat ccc ttt ggt gac gat tac ggc 1248

Lys Asn Cys Ile Ile Leu Met Asp Tyr Pro Phe Gly Asp Asp Tyr Gly

405 410 415

atc gaa gcc cta att acg ttg gaa caa gag aag atg ccc gcc ttc gaa 1296

Ile Glu Ala Leu Ile Thr Leu Glu Gln Glu Lys Met Pro Ala Phe Glu

420 425 430

aac aac gag ctg ctg cta agc ttc gcc agc aac taa 1332

Asn Asn Glu Leu Leu Leu Ser Phe Ala Ser Asn

435 440

<210> 13

<211> 443

<212> PRT

<213> CrMAT，针对其在酿酒酵母中的表达经密码子优化

<400> 13

Met Asp Ser Ile Thr Met Val Glu Thr Glu Thr Leu Ser Lys Thr Leu

1 5 10 15

Ile Lys Pro Ser Ser Pro Thr Pro Gln Ser Leu Ser His Tyr Asn Leu

20 25 30

Ser Tyr Asn Asp Gln Asn Ile Tyr Pro Glu Tyr Ile Phe Ala Gly Phe

35 40 45

Phe Tyr Ser Asn Pro Asp Gly His Glu Ile Ser Thr Ile Arg Glu Gln

50 55 60

Leu Gln Asn Ser Leu Ser Lys Thr Leu Val Ser Tyr Tyr Pro Phe Ala

65 70 75 80

Gly Lys Val Val Lys Asn Asp Tyr Ile His Cys Asn Asp Asp Gly Ile

85 90 95

Glu Phe Val Asp Val Arg Ile His Cys Arg Met Asn Asp Ile Leu Lys

100 105 110

Pro Glu Leu Arg Ser Tyr Ala Ser Glu Leu Ile Arg Pro Asn Arg Ser

115 120 125

Thr Val Gly Ser Glu Asp Ser Thr Ala Leu Val Gln Leu Ser His Phe

130 135 140

Asp Cys Gly Gly Val Ala Val Ala Phe Gly Ile Ser His Lys Val Ala

145 150 155 160

Asp Ala Ala Thr Ile Leu Ser Phe Ile Lys Asp Trp Ala Ala Ser Thr

165 170 175

Cys Asp Leu Ser Ser Ser His Asp Val Ser Thr Pro Val Leu Val Ser

180 185 190

Asp Ser Ile Phe Pro Arg Gln Asp Asn Ile Ile Cys Gly Gln Phe Pro

195 200 205

Ala Ser Pro Asn Cys Val Arg Lys Arg Phe Leu Phe Ser Pro Glu Ala

210 215 220

Ile Glu Arg Leu Lys Ser Lys Ala Ile Glu Phe Gly Ile Glu Lys Pro

225 230 235 240

Thr Arg Val Glu Val Leu Thr Ala Phe Leu Cys Arg Cys Ala Thr Val

245 250 255

Ala Gly Lys Ser Ala Ala Lys Asn Asn Asn Cys Gly Gln Ser Leu Pro

260 265 270

Phe Ala Val Ile Gln Ala Val Asn Leu Arg Pro Leu Leu Glu Leu Pro

275 280 285

Lys Asn Ser Val Gly Asn Leu Ile Ser Ile Tyr Phe Ser Thr Ile Lys

290 295 300

Glu Asn Asp Thr Val Asn Ile Glu Gln Glu Phe Thr Lys Leu Val Ile

305 310 315 320

Gly Glu Leu Arg Lys Ala Lys Asp Lys Leu Lys Asn Leu Ser Gln Glu

325 330 335

Lys Leu Asn Tyr Val Ala Arg Met Gln Asp Phe Ala Asn Cys Leu Lys

340 345 350

Glu Leu Asp Ile Ser Ser Phe Phe Asp Met Glu Asn Val Asp Ile Asp

355 360 365

Ala Tyr Leu Phe Ser Ser Trp Cys Arg Phe Pro Phe Tyr Asp Ile Asp

370 375 380

Phe Gly Leu Gly Lys Pro Ile Trp Val Cys Met Phe Gln Pro His Phe

385 390 395 400

Lys Asn Cys Ile Ile Leu Met Asp Tyr Pro Phe Gly Asp Asp Tyr Gly

405 410 415

Ile Glu Ala Leu Ile Thr Leu Glu Gln Glu Lys Met Pro Ala Phe Glu

420 425 430

Asn Asn Glu Leu Leu Leu Ser Phe Ala Ser Asn

435 440

<210> 14

<211> 1254

<212> DNA

<213> LiAAT-4 DNA序列，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1254)

<400> 14

atg gcc atg ata atc acg aag caa ata tta aga cct agt tcc ccg acg 48

Met Ala Met Ile Ile Thr Lys Gln Ile Leu Arg Pro Ser Ser Pro Thr

1 5 10 15

ccc caa gcc ttt aag aat cac aag ctg tcc tac tta gac caa ata cag 96

Pro Gln Ala Phe Lys Asn His Lys Leu Ser Tyr Leu Asp Gln Ile Gln

20 25 30

gcg cct att tac ata cct ttg ttg ttc ttc tat aag aac gag gag tca 144

Ala Pro Ile Tyr Ile Pro Leu Leu Phe Phe Tyr Lys Asn Glu Glu Ser

35 40 45

aaa tac cca gac cag atc tcg caa aga ttt aag cag agt ttg tcc gaa 192

Lys Tyr Pro Asp Gln Ile Ser Gln Arg Phe Lys Gln Ser Leu Ser Glu

50 55 60

att ttg aca ata ttc tac cct ttg gct ggt acg atg agg cat aac tcg 240

Ile Leu Thr Ile Phe Tyr Pro Leu Ala Gly Thr Met Arg His Asn Ser

65 70 75 80

ttc gtg gac tgc aat gac agg ggt gtc gaa ttt gta gag gtc agg gtc 288

Phe Val Asp Cys Asn Asp Arg Gly Val Glu Phe Val Glu Val Arg Val

85 90 95

cat gcg aga cta gcc cag ttc att caa gat cct aag atg gaa gag ctt 336

His Ala Arg Leu Ala Gln Phe Ile Gln Asp Pro Lys Met Glu Glu Leu

100 105 110

aag caa ttg ata cct gtg gat tgt ata tct cac act gac gat gat ttc 384

Lys Gln Leu Ile Pro Val Asp Cys Ile Ser His Thr Asp Asp Asp Phe

115 120 125

tta ttg cta gtc aag att agc tat ttt gac tgc ggt gag gtc gtc gtt 432

Leu Leu Leu Val Lys Ile Ser Tyr Phe Asp Cys Gly Glu Val Val Val

130 135 140

gga gtc tgc atg tcg cac aaa att ggt gac gga att agt ctg gca gcg 480

Gly Val Cys Met Ser His Lys Ile Gly Asp Gly Ile Ser Leu Ala Ala

145 150 155 160

ttt atg aac gct tgg gca gca acg tgt agg gga gaa tcg tct agt gag 528

Phe Met Asn Ala Trp Ala Ala Thr Cys Arg Gly Glu Ser Ser Ser Glu

165 170 175

atc att cac cca tct ttt gat ctt gct tta cac ttt ccg cct aaa gac 576

Ile Ile His Pro Ser Phe Asp Leu Ala Leu His Phe Pro Pro Lys Asp

180 185 190

cac ttg tct tca gca tcc tca ttc cgt gtg gcc ata gcc cag gag aac 624

His Leu Ser Ser Ala Ser Ser Phe Arg Val Ala Ile Ala Gln Glu Asn

195 200 205

atc atg acc aag agg cta gta ttt gat aga gaa aag ttg gag aag ctg 672

Ile Met Thr Lys Arg Leu Val Phe Asp Arg Glu Lys Leu Glu Lys Leu

210 215 220

cgt aag aga atc gct gcc agt tct gat ggg gtg aga gac cct agc aga 720

Arg Lys Arg Ile Ala Ala Ser Ser Asp Gly Val Arg Asp Pro Ser Arg

225 230 235 240

gtt gaa gct gta tct gtc ttt att tgg aaa agc tta att gaa gcc cac 768

Val Glu Ala Val Ser Val Phe Ile Trp Lys Ser Leu Ile Glu Ala His

245 250 255

aag gcc gag tca cac atg act gag aca cca gcc gtt tct att gct agc 816

Lys Ala Glu Ser His Met Thr Glu Thr Pro Ala Val Ser Ile Ala Ser

260 265 270

cac gcc gtg aac tta agg cct aga aca gtc cca caa atg gac caa act 864

His Ala Val Asn Leu Arg Pro Arg Thr Val Pro Gln Met Asp Gln Thr

275 280 285

ttc ggt aac tgc tac gct ccc gct tcg gcc gtt gtc tcc tgg gat gaa 912

Phe Gly Asn Cys Tyr Ala Pro Ala Ser Ala Val Val Ser Trp Asp Glu

290 295 300

gac tac gta cat cac agt cgt ttg agg gcg gcc ctt aga gaa atc gac 960

Asp Tyr Val His His Ser Arg Leu Arg Ala Ala Leu Arg Glu Ile Asp

305 310 315 320

gac gac tac att aat aag gtc ttg aaa gcc gac aat aat tat tta acg 1008

Asp Asp Tyr Ile Asn Lys Val Leu Lys Ala Asp Asn Asn Tyr Leu Thr

325 330 335

cag gat caa att ggt gac ttg ttt aaa cca gaa aac tca gtt cta agt 1056

Gln Asp Gln Ile Gly Asp Leu Phe Lys Pro Glu Asn Ser Val Leu Ser

340 345 350

tcg tgg tgg cgt ttc cca gtt tac aag gta gac ttt gga tgg ggt aag 1104

Ser Trp Trp Arg Phe Pro Val Tyr Lys Val Asp Phe Gly Trp Gly Lys

355 360 365

cca gtt tgg gtt tct acg acg acc atc caa tac atg aac ttg att ata 1152

Pro Val Trp Val Ser Thr Thr Thr Ile Gln Tyr Met Asn Leu Ile Ile

370 375 380

ttt act tcg acg ccc tca gaa gac ggc ata gag gcg tgg gta act act 1200

Phe Thr Ser Thr Pro Ser Glu Asp Gly Ile Glu Ala Trp Val Thr Thr

385 390 395 400

act cac aat ttc ttc caa gtt ctg cag gca aac tat aat aaa ctt gac 1248

Thr His Asn Phe Phe Gln Val Leu Gln Ala Asn Tyr Asn Lys Leu Asp

405 410 415

acg taa 1254

Thr

<210> 15

<211> 417

<212> PRT

<213> LiAAT-4 DNA序列，针对其在酿酒酵母中的表达经密码子优化

<400> 15

Met Ala Met Ile Ile Thr Lys Gln Ile Leu Arg Pro Ser Ser Pro Thr

1 5 10 15

Pro Gln Ala Phe Lys Asn His Lys Leu Ser Tyr Leu Asp Gln Ile Gln

20 25 30

Ala Pro Ile Tyr Ile Pro Leu Leu Phe Phe Tyr Lys Asn Glu Glu Ser

35 40 45

Lys Tyr Pro Asp Gln Ile Ser Gln Arg Phe Lys Gln Ser Leu Ser Glu

50 55 60

Ile Leu Thr Ile Phe Tyr Pro Leu Ala Gly Thr Met Arg His Asn Ser

65 70 75 80

Phe Val Asp Cys Asn Asp Arg Gly Val Glu Phe Val Glu Val Arg Val

85 90 95

His Ala Arg Leu Ala Gln Phe Ile Gln Asp Pro Lys Met Glu Glu Leu

100 105 110

Lys Gln Leu Ile Pro Val Asp Cys Ile Ser His Thr Asp Asp Asp Phe

115 120 125

Leu Leu Leu Val Lys Ile Ser Tyr Phe Asp Cys Gly Glu Val Val Val

130 135 140

Gly Val Cys Met Ser His Lys Ile Gly Asp Gly Ile Ser Leu Ala Ala

145 150 155 160

Phe Met Asn Ala Trp Ala Ala Thr Cys Arg Gly Glu Ser Ser Ser Glu

165 170 175

Ile Ile His Pro Ser Phe Asp Leu Ala Leu His Phe Pro Pro Lys Asp

180 185 190

His Leu Ser Ser Ala Ser Ser Phe Arg Val Ala Ile Ala Gln Glu Asn

195 200 205

Ile Met Thr Lys Arg Leu Val Phe Asp Arg Glu Lys Leu Glu Lys Leu

210 215 220

Arg Lys Arg Ile Ala Ala Ser Ser Asp Gly Val Arg Asp Pro Ser Arg

225 230 235 240

Val Glu Ala Val Ser Val Phe Ile Trp Lys Ser Leu Ile Glu Ala His

245 250 255

Lys Ala Glu Ser His Met Thr Glu Thr Pro Ala Val Ser Ile Ala Ser

260 265 270

His Ala Val Asn Leu Arg Pro Arg Thr Val Pro Gln Met Asp Gln Thr

275 280 285

Phe Gly Asn Cys Tyr Ala Pro Ala Ser Ala Val Val Ser Trp Asp Glu

290 295 300

Asp Tyr Val His His Ser Arg Leu Arg Ala Ala Leu Arg Glu Ile Asp

305 310 315 320

Asp Asp Tyr Ile Asn Lys Val Leu Lys Ala Asp Asn Asn Tyr Leu Thr

325 330 335

Gln Asp Gln Ile Gly Asp Leu Phe Lys Pro Glu Asn Ser Val Leu Ser

340 345 350

Ser Trp Trp Arg Phe Pro Val Tyr Lys Val Asp Phe Gly Trp Gly Lys

355 360 365

Pro Val Trp Val Ser Thr Thr Thr Ile Gln Tyr Met Asn Leu Ile Ile

370 375 380

Phe Thr Ser Thr Pro Ser Glu Asp Gly Ile Glu Ala Trp Val Thr Thr

385 390 395 400

Thr His Asn Phe Phe Gln Val Leu Gln Ala Asn Tyr Asn Lys Leu Asp

405 410 415

Thr

<210> 16

<211> 1485

<212> DNA

<213> FgaAT，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1485)

<400> 16

atg aag aag cag gtc act ttt aaa cct ttc aga ttg tca cca gtc gat 48

Met Lys Lys Gln Val Thr Phe Lys Pro Phe Arg Leu Ser Pro Val Asp

1 5 10 15

cat agt tta cct aag gtt tac atc ttc aag tct cta tat ttc aga ggg 96

His Ser Leu Pro Lys Val Tyr Ile Phe Lys Ser Leu Tyr Phe Arg Gly

20 25 30

gta gat gac act ggt tct ctt agt aga cta cag gat ggc att gat cgt 144

Val Asp Asp Thr Gly Ser Leu Ser Arg Leu Gln Asp Gly Ile Asp Arg

35 40 45

ttg ata tct tgt ctg cct ttc ctg tca ggc gag gtg gtt cca tgt gca 192

Leu Ile Ser Cys Leu Pro Phe Leu Ser Gly Glu Val Val Pro Cys Ala

50 55 60

gac ata cca gat aag gtc ggt gta ctt caa gtt cag atg cct tgc cct 240

Asp Ile Pro Asp Lys Val Gly Val Leu Gln Val Gln Met Pro Cys Pro

65 70 75 80

tcc ttg caa gaa att cca atg ctg cta gtc aaa agt tat cca aac cat 288

Ser Leu Gln Glu Ile Pro Met Leu Leu Val Lys Ser Tyr Pro Asn His

85 90 95

aca tgg cca gca gct tct acc tcg gaa aga tgg aga aac acc gct ttg 336

Thr Trp Pro Ala Ala Ser Thr Ser Glu Arg Trp Arg Asn Thr Ala Leu

100 105 110

cta gat cag tcg tat agg cca ttg ccg gat ttt ata ccc cca tca aag 384

Leu Asp Gln Ser Tyr Arg Pro Leu Pro Asp Phe Ile Pro Pro Ser Lys

115 120 125

cct aga cct gtt ttg aga ttt cag gcg aac ttc ctg gct gat ggt tta 432

Pro Arg Pro Val Leu Arg Phe Gln Ala Asn Phe Leu Ala Asp Gly Leu

130 135 140

atg cta tgt atg gga tat aat cat tct gta ttc gac gga aca ggt gcg 480

Met Leu Cys Met Gly Tyr Asn His Ser Val Phe Asp Gly Thr Gly Ala

145 150 155 160

ggg aac att ctg gaa atg ttg gct gat tgt tgt aga gct aat cca aac 528

Gly Asn Ile Leu Glu Met Leu Ala Asp Cys Cys Arg Ala Asn Pro Asn

165 170 175

tcc atc tta gca ctg cca aca aac ggt gac ata gag agc gag ttg aga 576

Ser Ile Leu Ala Leu Pro Thr Asn Gly Asp Ile Glu Ser Glu Leu Arg

180 185 190

ggt tta ctg tcc agt cca ggt gta gca gtt gca aat gcc tct cag gaa 624

Gly Leu Leu Ser Ser Pro Gly Val Ala Val Ala Asn Ala Ser Gln Glu

195 200 205

gcc tac gca att aat tgt gca cac acg gaa gta gaa ccc gaa ccc agt 672

Ala Tyr Ala Ile Asn Cys Ala His Thr Glu Val Glu Pro Glu Pro Ser

210 215 220

tca gct atg ctt tac tgt tgg cct ttc ttg ctg agt tct gag aag att 720

Ser Ala Met Leu Tyr Cys Trp Pro Phe Leu Leu Ser Ser Glu Lys Ile

225 230 235 240

gaa tgt tta cag gaa gca tgt aat agt tta cta cca cat atc gtt cgt 768

Glu Cys Leu Gln Glu Ala Cys Asn Ser Leu Leu Pro His Ile Val Arg

245 250 255

ttg tac tct ggt acg cag agt tcg ctt ata aac caa gat acg aac tgg 816

Leu Tyr Ser Gly Thr Gln Ser Ser Leu Ile Asn Gln Asp Thr Asn Trp

260 265 270

cca cat att ctt tca tca aac gac gtg cta acc gct ctt tta gct gtt 864

Pro His Ile Leu Ser Ser Asn Asp Val Leu Thr Ala Leu Leu Ala Val

275 280 285

tca att gaa aag gct agg gaa gca aca ggt gcc ctt ggt cat atg agc 912

Ser Ile Glu Lys Ala Arg Glu Ala Thr Gly Ala Leu Gly His Met Ser

290 295 300

aga tca ttg gct atg gct gtt aac cta cgt gag cgt tta aag cct atg 960

Arg Ser Leu Ala Met Ala Val Asn Leu Arg Glu Arg Leu Lys Pro Met

305 310 315 320

cca aga cac tat ttg ggt aac cta gtt acc act gtg tgg gta tca cat 1008

Pro Arg His Tyr Leu Gly Asn Leu Val Thr Thr Val Trp Val Ser His

325 330 335

cac cgt cct gcc gtt aag gac ctt gaa aca atg gtt tta cca gtg cca 1056

His Arg Pro Ala Val Lys Asp Leu Glu Thr Met Val Leu Pro Val Pro

340 345 350

gca tgc aat agg cac gaa ata gac aga gac gac ttg ttg tgg ata acc 1104

Ala Cys Asn Arg His Glu Ile Asp Arg Asp Asp Leu Leu Trp Ile Thr

355 360 365

cac gtg gca ttc cgt att aga tta ggg ctg aac gca ata aac gaa gaa 1152

His Val Ala Phe Arg Ile Arg Leu Gly Leu Asn Ala Ile Asn Glu Glu

370 375 380

cat att aga ggt ctg atc cac tac ttg cat tcc caa gac gat tgg gaa 1200

His Ile Arg Gly Leu Ile His Tyr Leu His Ser Gln Asp Asp Trp Glu

385 390 395 400

cag att gga ata cat ttc acc gat cca att ttc att tct tcc tgg cgt 1248

Gln Ile Gly Ile His Phe Thr Asp Pro Ile Phe Ile Ser Ser Trp Arg

405 410 415

cac ctt aag gtc tat gaa cta gac ttc gga cct act atc ggt cat gct 1296

His Leu Lys Val Tyr Glu Leu Asp Phe Gly Pro Thr Ile Gly His Ala

420 425 430

gaa cac ttt gag atg gat gtc ggc acc acc gat ggt gtg tgt gtt gtt 1344

Glu His Phe Glu Met Asp Val Gly Thr Thr Asp Gly Val Cys Val Val

435 440 445

atg cct gct aac acc aga gcc gtc ggc aag act aag aag gct cct tgg 1392

Met Pro Ala Asn Thr Arg Ala Val Gly Lys Thr Lys Lys Ala Pro Trp

450 455 460

gac att aga atc gtg tta aac ccc gaa gtg tta cag gca cta ata gct 1440

Asp Ile Arg Ile Val Leu Asn Pro Glu Val Leu Gln Ala Leu Ile Ala

465 470 475 480

agt gcc atc ttt ggt tgg gct atg gtc aag gac gct tcg aca taa 1485

Ser Ala Ile Phe Gly Trp Ala Met Val Lys Asp Ala Ser Thr

485 490

<210> 17

<211> 494

<212> PRT

<213> FgaAT，针对其在酿酒酵母中的表达经密码子优化

<400> 17

Met Lys Lys Gln Val Thr Phe Lys Pro Phe Arg Leu Ser Pro Val Asp

1 5 10 15

His Ser Leu Pro Lys Val Tyr Ile Phe Lys Ser Leu Tyr Phe Arg Gly

20 25 30

Val Asp Asp Thr Gly Ser Leu Ser Arg Leu Gln Asp Gly Ile Asp Arg

35 40 45

Leu Ile Ser Cys Leu Pro Phe Leu Ser Gly Glu Val Val Pro Cys Ala

50 55 60

Asp Ile Pro Asp Lys Val Gly Val Leu Gln Val Gln Met Pro Cys Pro

65 70 75 80

Ser Leu Gln Glu Ile Pro Met Leu Leu Val Lys Ser Tyr Pro Asn His

85 90 95

Thr Trp Pro Ala Ala Ser Thr Ser Glu Arg Trp Arg Asn Thr Ala Leu

100 105 110

Leu Asp Gln Ser Tyr Arg Pro Leu Pro Asp Phe Ile Pro Pro Ser Lys

115 120 125

Pro Arg Pro Val Leu Arg Phe Gln Ala Asn Phe Leu Ala Asp Gly Leu

130 135 140

Met Leu Cys Met Gly Tyr Asn His Ser Val Phe Asp Gly Thr Gly Ala

145 150 155 160

Gly Asn Ile Leu Glu Met Leu Ala Asp Cys Cys Arg Ala Asn Pro Asn

165 170 175

Ser Ile Leu Ala Leu Pro Thr Asn Gly Asp Ile Glu Ser Glu Leu Arg

180 185 190

Gly Leu Leu Ser Ser Pro Gly Val Ala Val Ala Asn Ala Ser Gln Glu

195 200 205

Ala Tyr Ala Ile Asn Cys Ala His Thr Glu Val Glu Pro Glu Pro Ser

210 215 220

Ser Ala Met Leu Tyr Cys Trp Pro Phe Leu Leu Ser Ser Glu Lys Ile

225 230 235 240

Glu Cys Leu Gln Glu Ala Cys Asn Ser Leu Leu Pro His Ile Val Arg

245 250 255

Leu Tyr Ser Gly Thr Gln Ser Ser Leu Ile Asn Gln Asp Thr Asn Trp

260 265 270

Pro His Ile Leu Ser Ser Asn Asp Val Leu Thr Ala Leu Leu Ala Val

275 280 285

Ser Ile Glu Lys Ala Arg Glu Ala Thr Gly Ala Leu Gly His Met Ser

290 295 300

Arg Ser Leu Ala Met Ala Val Asn Leu Arg Glu Arg Leu Lys Pro Met

305 310 315 320

Pro Arg His Tyr Leu Gly Asn Leu Val Thr Thr Val Trp Val Ser His

325 330 335

His Arg Pro Ala Val Lys Asp Leu Glu Thr Met Val Leu Pro Val Pro

340 345 350

Ala Cys Asn Arg His Glu Ile Asp Arg Asp Asp Leu Leu Trp Ile Thr

355 360 365

His Val Ala Phe Arg Ile Arg Leu Gly Leu Asn Ala Ile Asn Glu Glu

370 375 380

His Ile Arg Gly Leu Ile His Tyr Leu His Ser Gln Asp Asp Trp Glu

385 390 395 400

Gln Ile Gly Ile His Phe Thr Asp Pro Ile Phe Ile Ser Ser Trp Arg

405 410 415

His Leu Lys Val Tyr Glu Leu Asp Phe Gly Pro Thr Ile Gly His Ala

420 425 430

Glu His Phe Glu Met Asp Val Gly Thr Thr Asp Gly Val Cys Val Val

435 440 445

Met Pro Ala Asn Thr Arg Ala Val Gly Lys Thr Lys Lys Ala Pro Trp

450 455 460

Asp Ile Arg Ile Val Leu Asn Pro Glu Val Leu Gln Ala Leu Ile Ala

465 470 475 480

Ser Ala Ile Phe Gly Trp Ala Met Val Lys Asp Ala Ser Thr

485 490

<210> 18

<211> 1548

<212> DNA

<213> GAO81666.1，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1548)

<400> 18

atg gaa gaa cat acc aga tcc ttt gag cca ttt gac ctg gcc tgc tta 48

Met Glu Glu His Thr Arg Ser Phe Glu Pro Phe Asp Leu Ala Cys Leu

1 5 10 15

gat cat act gtg ggg ccg gtt ttc atg aat ttc ttc tta agt ttc aag 96

Asp His Thr Val Gly Pro Val Phe Met Asn Phe Phe Leu Ser Phe Lys

20 25 30

cct gcc aag atc gaa gag agt ctt atc tca att gaa gaa ggt gta act 144

Pro Ala Lys Ile Glu Glu Ser Leu Ile Ser Ile Glu Glu Gly Val Thr

35 40 45

agg tta gtc aac aga ctg cca ttc ctg gcc ggt gac gtt gtt aat tct 192

Arg Leu Val Asn Arg Leu Pro Phe Leu Ala Gly Asp Val Val Asn Ser

50 55 60

gaa aac gta gac ggt cgt gtt aat gta atg agg att cag cca agc tcg 240

Glu Asn Val Asp Gly Arg Val Asn Val Met Arg Ile Gln Pro Ser Ser

65 70 75 80

aca ttg att aga gag att ccc atg cta cac aca aag cac cat cct cat 288

Thr Leu Ile Arg Glu Ile Pro Met Leu His Thr Lys His His Pro His

85 90 95

cat att ttg ccg aat aca cct aga cag att aac cca ggg tcc gac cag 336

His Ile Leu Pro Asn Thr Pro Arg Gln Ile Asn Pro Gly Ser Asp Gln

100 105 110

gat caa agg ttt gct ccc ttg gac gat tcg tac gta cca cca gtg tct 384

Asp Gln Arg Phe Ala Pro Leu Asp Asp Ser Tyr Val Pro Pro Val Ser

115 120 125

tta tta cct ctt gct ccg ggc cca agg ccc gta gta aga ttc caa act 432

Leu Leu Pro Leu Ala Pro Gly Pro Arg Pro Val Val Arg Phe Gln Thr

130 135 140

aat gtt gta att gac ggg att gtg ctt gca ctt ggg ttc cac cac tca 480

Asn Val Val Ile Asp Gly Ile Val Leu Ala Leu Gly Phe His His Ser

145 150 155 160

gta ttt gat gct acc gga gtg ggt ttg ttg atc gaa atg ttg gcc aca 528

Val Phe Asp Ala Thr Gly Val Gly Leu Leu Ile Glu Met Leu Ala Thr

165 170 175

tgt tgt tca agc gat tgt cct gcc cta tca tcc cat att gaa ctg gaa 576

Cys Cys Ser Ser Asp Cys Pro Ala Leu Ser Ser His Ile Glu Leu Glu

180 185 190

gaa gaa ata agg cta agg cgt tct gtg gac aag ata ggt aac ggc gct 624

Glu Glu Ile Arg Leu Arg Arg Ser Val Asp Lys Ile Gly Asn Gly Ala

195 200 205

act gat tta gca tcc caa aga gat gaa gtt cag gac cca aat ggc agt 672

Thr Asp Leu Ala Ser Gln Arg Asp Glu Val Gln Asp Pro Asn Gly Ser

210 215 220

atc act gag tcc cca gct cat gtg cct atg ggt gac agt agc tgg gta 720

Ile Thr Glu Ser Pro Ala His Val Pro Met Gly Asp Ser Ser Trp Val

225 230 235 240

ccc cct aaa cta tct gtt tac tcc ttt aat tta tcg gct gcc ggt ttg 768

Pro Pro Lys Leu Ser Val Tyr Ser Phe Asn Leu Ser Ala Ala Gly Leu

245 250 255

gct cat ttg aag aca gcc tgt aac aag tta ttg ccc gct atc cat gcg 816

Ala His Leu Lys Thr Ala Cys Asn Lys Leu Leu Pro Ala Ile His Ala

260 265 270

agt caa aat ggt tca cca caa agt gcg gaa tca gaa ggt gag aaa tta 864

Ser Gln Asn Gly Ser Pro Gln Ser Ala Glu Ser Glu Gly Glu Lys Leu

275 280 285

cag caa gaa ttc gtt tcg acg aat gat gtt cta acg gct ttg ttg gct 912

Gln Gln Glu Phe Val Ser Thr Asn Asp Val Leu Thr Ala Leu Leu Ala

290 295 300

act tcc att cac caa gcg agg tct aga gtt act gaa acg gaa tta gtt 960

Thr Ser Ile His Gln Ala Arg Ser Arg Val Thr Glu Thr Glu Leu Val

305 310 315 320

ccg aca aaa gcg aaa ctt gcg atg gcc gtt aat ttg agg gaa agg gcc 1008

Pro Thr Lys Ala Lys Leu Ala Met Ala Val Asn Leu Arg Glu Arg Ala

325 330 335

agt tcg tta ccc aag act tat ttg ggg aac tcg cta aca gtt aca gaa 1056

Ser Ser Leu Pro Lys Thr Tyr Leu Gly Asn Ser Leu Thr Val Thr Glu

340 345 350

gct ttt gtg tat tct tta gct gct acc gac ggt ttc gat ggt gtc ccc 1104

Ala Phe Val Tyr Ser Leu Ala Ala Thr Asp Gly Phe Asp Gly Val Pro

355 360 365

gag aga cat tat cat cct gac ttg cgt aac gcc ttg cta tta gaa att 1152

Glu Arg His Tyr His Pro Asp Leu Arg Asn Ala Leu Leu Leu Glu Ile

370 375 380

gct aga gtt gca ttg caa ttg aga aaa ggg ctg gca gcg ata gat gac 1200

Ala Arg Val Ala Leu Gln Leu Arg Lys Gly Leu Ala Ala Ile Asp Asp

385 390 395 400

gcg tat ttt agg caa ttt gtg tct aga ttg agg gct aat cta gac tgg 1248

Ala Tyr Phe Arg Gln Phe Val Ser Arg Leu Arg Ala Asn Leu Asp Trp

405 410 415

tca cag tta ggt gca aac ctg cct gac act atg gtg tca agc tgg aga 1296

Ser Gln Leu Gly Ala Asn Leu Pro Asp Thr Met Val Ser Ser Trp Arg

420 425 430

cat ttg aaa gta tat cgt tta gac ttc ggt gct aga ttg ggg aga gtt 1344

His Leu Lys Val Tyr Arg Leu Asp Phe Gly Ala Arg Leu Gly Arg Val

435 440 445

gta gag ttc cat cca caa acc gct ttg gtt gac ggt att tgt atc atc 1392

Val Glu Phe His Pro Gln Thr Ala Leu Val Asp Gly Ile Cys Ile Ile

450 455 460

caa cct gaa agg ata gcc cat gag gat gat tca gct gat atg gct cca 1440

Gln Pro Glu Arg Ile Ala His Glu Asp Asp Ser Ala Asp Met Ala Pro

465 470 475 480

gaa tct ggg tgg gaa gtt tgt gta aca tta caa agc gat gcc atg gag 1488

Glu Ser Gly Trp Glu Val Cys Val Thr Leu Gln Ser Asp Ala Met Glu

485 490 495

tgc ttc tta aga ggt ggg ttg ttc aca agt ctt tct caa ggt gca att 1536

Cys Phe Leu Arg Gly Gly Leu Phe Thr Ser Leu Ser Gln Gly Ala Ile

500 505 510

aga aga gtt taa 1548

Arg Arg Val

515

<210> 19

<211> 515

<212> PRT

<213> GAO81666.1，针对其在酿酒酵母中的表达经密码子优化

<400> 19

Met Glu Glu His Thr Arg Ser Phe Glu Pro Phe Asp Leu Ala Cys Leu

1 5 10 15

Asp His Thr Val Gly Pro Val Phe Met Asn Phe Phe Leu Ser Phe Lys

20 25 30

Pro Ala Lys Ile Glu Glu Ser Leu Ile Ser Ile Glu Glu Gly Val Thr

35 40 45

Arg Leu Val Asn Arg Leu Pro Phe Leu Ala Gly Asp Val Val Asn Ser

50 55 60

Glu Asn Val Asp Gly Arg Val Asn Val Met Arg Ile Gln Pro Ser Ser

65 70 75 80

Thr Leu Ile Arg Glu Ile Pro Met Leu His Thr Lys His His Pro His

85 90 95

His Ile Leu Pro Asn Thr Pro Arg Gln Ile Asn Pro Gly Ser Asp Gln

100 105 110

Asp Gln Arg Phe Ala Pro Leu Asp Asp Ser Tyr Val Pro Pro Val Ser

115 120 125

Leu Leu Pro Leu Ala Pro Gly Pro Arg Pro Val Val Arg Phe Gln Thr

130 135 140

Asn Val Val Ile Asp Gly Ile Val Leu Ala Leu Gly Phe His His Ser

145 150 155 160

Val Phe Asp Ala Thr Gly Val Gly Leu Leu Ile Glu Met Leu Ala Thr

165 170 175

Cys Cys Ser Ser Asp Cys Pro Ala Leu Ser Ser His Ile Glu Leu Glu

180 185 190

Glu Glu Ile Arg Leu Arg Arg Ser Val Asp Lys Ile Gly Asn Gly Ala

195 200 205

Thr Asp Leu Ala Ser Gln Arg Asp Glu Val Gln Asp Pro Asn Gly Ser

210 215 220

Ile Thr Glu Ser Pro Ala His Val Pro Met Gly Asp Ser Ser Trp Val

225 230 235 240

Pro Pro Lys Leu Ser Val Tyr Ser Phe Asn Leu Ser Ala Ala Gly Leu

245 250 255

Ala His Leu Lys Thr Ala Cys Asn Lys Leu Leu Pro Ala Ile His Ala

260 265 270

Ser Gln Asn Gly Ser Pro Gln Ser Ala Glu Ser Glu Gly Glu Lys Leu

275 280 285

Gln Gln Glu Phe Val Ser Thr Asn Asp Val Leu Thr Ala Leu Leu Ala

290 295 300

Thr Ser Ile His Gln Ala Arg Ser Arg Val Thr Glu Thr Glu Leu Val

305 310 315 320

Pro Thr Lys Ala Lys Leu Ala Met Ala Val Asn Leu Arg Glu Arg Ala

325 330 335

Ser Ser Leu Pro Lys Thr Tyr Leu Gly Asn Ser Leu Thr Val Thr Glu

340 345 350

Ala Phe Val Tyr Ser Leu Ala Ala Thr Asp Gly Phe Asp Gly Val Pro

355 360 365

Glu Arg His Tyr His Pro Asp Leu Arg Asn Ala Leu Leu Leu Glu Ile

370 375 380

Ala Arg Val Ala Leu Gln Leu Arg Lys Gly Leu Ala Ala Ile Asp Asp

385 390 395 400

Ala Tyr Phe Arg Gln Phe Val Ser Arg Leu Arg Ala Asn Leu Asp Trp

405 410 415

Ser Gln Leu Gly Ala Asn Leu Pro Asp Thr Met Val Ser Ser Trp Arg

420 425 430

His Leu Lys Val Tyr Arg Leu Asp Phe Gly Ala Arg Leu Gly Arg Val

435 440 445

Val Glu Phe His Pro Gln Thr Ala Leu Val Asp Gly Ile Cys Ile Ile

450 455 460

Gln Pro Glu Arg Ile Ala His Glu Asp Asp Ser Ala Asp Met Ala Pro

465 470 475 480

Glu Ser Gly Trp Glu Val Cys Val Thr Leu Gln Ser Asp Ala Met Glu

485 490 495

Cys Phe Leu Arg Gly Gly Leu Phe Thr Ser Leu Ser Gln Gly Ala Ile

500 505 510

Arg Arg Val

515

<210> 20

<211> 1284

<212> DNA

<213> CfACT1-6 DNA序列，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1284)

<400> 20

atg aag gta gaa cgt ttc tca aga aag ttg ata aaa ccc cac acg cct 48

Met Lys Val Glu Arg Phe Ser Arg Lys Leu Ile Lys Pro His Thr Pro

1 5 10 15

act ccc gag aac tta aag aaa tat aaa ttg tct cta ttg gac aaa tgc 96

Thr Pro Glu Asn Leu Lys Lys Tyr Lys Leu Ser Leu Leu Asp Lys Cys

20 25 30

ctt gga cac gac aac ttc gcg atc gtg ttg ttt tac gaa tct aaa cca 144

Leu Gly His Asp Asn Phe Ala Ile Val Leu Phe Tyr Glu Ser Lys Pro

35 40 45

aga aat aag agt gag ctt gag gaa tcg tta gag aag gtc ttg gta gac 192

Arg Asn Lys Ser Glu Leu Glu Glu Ser Leu Glu Lys Val Leu Val Asp

50 55 60

ttt tat ccc ttg gct ggg cgt cac acg atg aac gac cat att gtc gac 240

Phe Tyr Pro Leu Ala Gly Arg His Thr Met Asn Asp His Ile Val Asp

65 70 75 80

tgc tcg gat gtc ggc gct gtc ttt gta gaa gcc gag gct tta gat gta 288

Cys Ser Asp Val Gly Ala Val Phe Val Glu Ala Glu Ala Leu Asp Val

85 90 95

gag ttg act atg gat gag tta gtc aag aac atg gaa gct caa act ata 336

Glu Leu Thr Met Asp Glu Leu Val Lys Asn Met Glu Ala Gln Thr Ile

100 105 110

cac cac ttg ttg cca aat cag tac ttc agt gca gat gct ccg aac ccg 384

His His Leu Leu Pro Asn Gln Tyr Phe Ser Ala Asp Ala Pro Asn Pro

115 120 125

ctg ctt tcc atc caa gtt acg cac ttt cct tcc ggt ggt ttg gct atc 432

Leu Leu Ser Ile Gln Val Thr His Phe Pro Ser Gly Gly Leu Ala Ile

130 135 140

gga atc gca gtc tcg cac gct gta ttt gat ggt ttc tct ttg ggc gtg 480

Gly Ile Ala Val Ser His Ala Val Phe Asp Gly Phe Ser Leu Gly Val

145 150 155 160

ttc gta gca gca tgg tca aag gcg acc atg aac ccg gat agg aaa atc 528

Phe Val Ala Ala Trp Ser Lys Ala Thr Met Asn Pro Asp Arg Lys Ile

165 170 175

aaa ata aca ccg tca ttc gac tta cca tca ctt ctg ccc tac aaa gac 576

Lys Ile Thr Pro Ser Phe Asp Leu Pro Ser Leu Leu Pro Tyr Lys Asp

180 185 190

gac aac ttt ggt ttg act gct gct gaa att gtc agc cag agc gag gac 624

Asp Asn Phe Gly Leu Thr Ala Ala Glu Ile Val Ser Gln Ser Glu Asp

195 200 205

atc gta gtt aag aga ttt atc ttc ggc aag gaa gcc atc acg agg ttg 672

Ile Val Val Lys Arg Phe Ile Phe Gly Lys Glu Ala Ile Thr Arg Leu

210 215 220

aga agt aag ctt agt cca aat agg aac ggg aag aaa ata tcc agg gtt 720

Arg Ser Lys Leu Ser Pro Asn Arg Asn Gly Lys Lys Ile Ser Arg Val

225 230 235 240

agg gtc gtt tgt gca gtc att gta aag gcc ttg atg gga ttg gag cgt 768

Arg Val Val Cys Ala Val Ile Val Lys Ala Leu Met Gly Leu Glu Arg

245 250 255

gcc aaa cac ggt aag acg cgt gat ttc tta att act caa tca att aac 816

Ala Lys His Gly Lys Thr Arg Asp Phe Leu Ile Thr Gln Ser Ile Asn

260 265 270

atg agg gag aga act aag gcg ccg ctg cag aaa cac gcc tgc ggc aac 864

Met Arg Glu Arg Thr Lys Ala Pro Leu Gln Lys His Ala Cys Gly Asn

275 280 285

tta gca gtc ttg agt tgc acg aga aga gta gag gcc gag gag atg atg 912

Leu Ala Val Leu Ser Cys Thr Arg Arg Val Glu Ala Glu Glu Met Met

290 295 300

gag tta cag aac cta gtt aat ttg atc ggc gac agt acc gaa aag gac 960

Glu Leu Gln Asn Leu Val Asn Leu Ile Gly Asp Ser Thr Glu Lys Asp

305 310 315 320

atc gcc gac ttt gca gaa tta tta tca cca gat caa gtg ggc aga gac 1008

Ile Ala Asp Phe Ala Glu Leu Leu Ser Pro Asp Gln Val Gly Arg Asp

325 330 335

att ata atc aag atg atg aag tca ttc atg cag ttc ttg gac aat gac 1056

Ile Ile Ile Lys Met Met Lys Ser Phe Met Gln Phe Leu Asp Asn Asp

340 345 350

att tac agc gtg tgc ttt acc gat tgg tca aag ttt gaa ttt tac gaa 1104

Ile Tyr Ser Val Cys Phe Thr Asp Trp Ser Lys Phe Glu Phe Tyr Glu

355 360 365

gcg gac ttc ggc ttc ggg aag ccg gtt tgg atg gca gca ggc cca cag 1152

Ala Asp Phe Gly Phe Gly Lys Pro Val Trp Met Ala Ala Gly Pro Gln

370 375 380

aga ccc att atc tct act gca ata ttg atg tca gac agg gaa ggc gac 1200

Arg Pro Ile Ile Ser Thr Ala Ile Leu Met Ser Asp Arg Glu Gly Asp

385 390 395 400

ggt atc gag gct tgg tta cat ttg aat aag aac gac atg ttg att ttc 1248

Gly Ile Glu Ala Trp Leu His Leu Asn Lys Asn Asp Met Leu Ile Phe

405 410 415

gaa caa gac gag gaa atc aag tta ttc act aca taa 1284

Glu Gln Asp Glu Glu Ile Lys Leu Phe Thr Thr

420 425

<210> 21

<211> 427

<212> PRT

<213> CfACT1-6 DNA序列，针对其在酿酒酵母中的表达经密码子优化

<400> 21

Met Lys Val Glu Arg Phe Ser Arg Lys Leu Ile Lys Pro His Thr Pro

1 5 10 15

Thr Pro Glu Asn Leu Lys Lys Tyr Lys Leu Ser Leu Leu Asp Lys Cys

20 25 30

Leu Gly His Asp Asn Phe Ala Ile Val Leu Phe Tyr Glu Ser Lys Pro

35 40 45

Arg Asn Lys Ser Glu Leu Glu Glu Ser Leu Glu Lys Val Leu Val Asp

50 55 60

Phe Tyr Pro Leu Ala Gly Arg His Thr Met Asn Asp His Ile Val Asp

65 70 75 80

Cys Ser Asp Val Gly Ala Val Phe Val Glu Ala Glu Ala Leu Asp Val

85 90 95

Glu Leu Thr Met Asp Glu Leu Val Lys Asn Met Glu Ala Gln Thr Ile

100 105 110

His His Leu Leu Pro Asn Gln Tyr Phe Ser Ala Asp Ala Pro Asn Pro

115 120 125

Leu Leu Ser Ile Gln Val Thr His Phe Pro Ser Gly Gly Leu Ala Ile

130 135 140

Gly Ile Ala Val Ser His Ala Val Phe Asp Gly Phe Ser Leu Gly Val

145 150 155 160

Phe Val Ala Ala Trp Ser Lys Ala Thr Met Asn Pro Asp Arg Lys Ile

165 170 175

Lys Ile Thr Pro Ser Phe Asp Leu Pro Ser Leu Leu Pro Tyr Lys Asp

180 185 190

Asp Asn Phe Gly Leu Thr Ala Ala Glu Ile Val Ser Gln Ser Glu Asp

195 200 205

Ile Val Val Lys Arg Phe Ile Phe Gly Lys Glu Ala Ile Thr Arg Leu

210 215 220

Arg Ser Lys Leu Ser Pro Asn Arg Asn Gly Lys Lys Ile Ser Arg Val

225 230 235 240

Arg Val Val Cys Ala Val Ile Val Lys Ala Leu Met Gly Leu Glu Arg

245 250 255

Ala Lys His Gly Lys Thr Arg Asp Phe Leu Ile Thr Gln Ser Ile Asn

260 265 270

Met Arg Glu Arg Thr Lys Ala Pro Leu Gln Lys His Ala Cys Gly Asn

275 280 285

Leu Ala Val Leu Ser Cys Thr Arg Arg Val Glu Ala Glu Glu Met Met

290 295 300

Glu Leu Gln Asn Leu Val Asn Leu Ile Gly Asp Ser Thr Glu Lys Asp

305 310 315 320

Ile Ala Asp Phe Ala Glu Leu Leu Ser Pro Asp Gln Val Gly Arg Asp

325 330 335

Ile Ile Ile Lys Met Met Lys Ser Phe Met Gln Phe Leu Asp Asn Asp

340 345 350

Ile Tyr Ser Val Cys Phe Thr Asp Trp Ser Lys Phe Glu Phe Tyr Glu

355 360 365

Ala Asp Phe Gly Phe Gly Lys Pro Val Trp Met Ala Ala Gly Pro Gln

370 375 380

Arg Pro Ile Ile Ser Thr Ala Ile Leu Met Ser Asp Arg Glu Gly Asp

385 390 395 400

Gly Ile Glu Ala Trp Leu His Leu Asn Lys Asn Asp Met Leu Ile Phe

405 410 415

Glu Gln Asp Glu Glu Ile Lys Leu Phe Thr Thr

420 425

<210> 22

<211> 1272

<212> DNA

<213> CfACT1-8，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1272)

<400> 22

atg aaa gtc gaa agg att tca cgt aaa ttc atc aag cca tat aca cct 48

Met Lys Val Glu Arg Ile Ser Arg Lys Phe Ile Lys Pro Tyr Thr Pro

1 5 10 15

aca cca cag aac ctt aag aag tac aag cta tcc ttg ctg gat aaa tgc 96

Thr Pro Gln Asn Leu Lys Lys Tyr Lys Leu Ser Leu Leu Asp Lys Cys

20 25 30

atg gga cac atg gac ttc gct gta gta ttg ttt tac gaa tca aag cca 144

Met Gly His Met Asp Phe Ala Val Val Leu Phe Tyr Glu Ser Lys Pro

35 40 45

aga aac aag aat gag ctg gaa gaa tca cta gag aaa gtg tta gtc gat 192

Arg Asn Lys Asn Glu Leu Glu Glu Ser Leu Glu Lys Val Leu Val Asp

50 55 60

ttc tat cca ttg gca ggc agg tat acc atg aac gac cac att gtc gat 240

Phe Tyr Pro Leu Ala Gly Arg Tyr Thr Met Asn Asp His Ile Val Asp

65 70 75 80

tgc agc gat gag ggc gcc gtt ttc gtt gag gca gag gcc cct aat gtt 288

Cys Ser Asp Glu Gly Ala Val Phe Val Glu Ala Glu Ala Pro Asn Val

85 90 95

gag ctt aca gtg gac cag ttg gtt aag aac atg gaa gcc cag aca atc 336

Glu Leu Thr Val Asp Gln Leu Val Lys Asn Met Glu Ala Gln Thr Ile

100 105 110

cac gac ttc tta cca gac caa tat ttt cct gct gac gca cca aat ccg 384

His Asp Phe Leu Pro Asp Gln Tyr Phe Pro Ala Asp Ala Pro Asn Pro

115 120 125

ttg cta agt att caa gta acg cac ttc cct tgt ggt ggt tta gct atc 432

Leu Leu Ser Ile Gln Val Thr His Phe Pro Cys Gly Gly Leu Ala Ile

130 135 140

ggg att gtt gtt agt cac gcg gtc ttt gat gga ttc tca ttg ggc gta 480

Gly Ile Val Val Ser His Ala Val Phe Asp Gly Phe Ser Leu Gly Val

145 150 155 160

ttc tta gcc gcc tgg agc aaa gct acc atg aac cct gag agg aag atc 528

Phe Leu Ala Ala Trp Ser Lys Ala Thr Met Asn Pro Glu Arg Lys Ile

165 170 175

gaa atc acc cct tcc ttc gat ttg cct agt ctt ctg ccc tac aag gat 576

Glu Ile Thr Pro Ser Phe Asp Leu Pro Ser Leu Leu Pro Tyr Lys Asp

180 185 190

gaa tct ttc ggt tta aat ttt agc gaa att gtc aaa gct gag aat atc 624

Glu Ser Phe Gly Leu Asn Phe Ser Glu Ile Val Lys Ala Glu Asn Ile

195 200 205

gta gtt aaa cgt ttg aat ttc ggg aaa gag gct att acg cgt ttg agg 672

Val Val Lys Arg Leu Asn Phe Gly Lys Glu Ala Ile Thr Arg Leu Arg

210 215 220

tcc aag ctg tct cct aac cag aat ggt aaa acc att tcc aga gtg agg 720

Ser Lys Leu Ser Pro Asn Gln Asn Gly Lys Thr Ile Ser Arg Val Arg

225 230 235 240

gta gtc tgc gcg gtg ata gtt aag gcg ttg atg gga ctg gag aga gca 768

Val Val Cys Ala Val Ile Val Lys Ala Leu Met Gly Leu Glu Arg Ala

245 250 255

aag act aga gac ttt atg ata tgt cag ggg atc aac atg aga gag agg 816

Lys Thr Arg Asp Phe Met Ile Cys Gln Gly Ile Asn Met Arg Glu Arg

260 265 270

acg aag gcg ccc ctg cag aag cat gcg tgt ggc aac cta gca gtt tcg 864

Thr Lys Ala Pro Leu Gln Lys His Ala Cys Gly Asn Leu Ala Val Ser

275 280 285

tct tac act aga agg gta gcg gca gcg gaa gca gaa gaa ctg cag tcc 912

Ser Tyr Thr Arg Arg Val Ala Ala Ala Glu Ala Glu Glu Leu Gln Ser

290 295 300

tta gtg aat ttg atc ggg gac tct atc gaa aag tca atc gca gac tac 960

Leu Val Asn Leu Ile Gly Asp Ser Ile Glu Lys Ser Ile Ala Asp Tyr

305 310 315 320

gct gat ata ctt tcg agt gat caa gat ggg aga cac atc att tcc act 1008

Ala Asp Ile Leu Ser Ser Asp Gln Asp Gly Arg His Ile Ile Ser Thr

325 330 335

atg atg aag agc ttt atg cag ttt gct gca cct gat ata aaa gcc att 1056

Met Met Lys Ser Phe Met Gln Phe Ala Ala Pro Asp Ile Lys Ala Ile

340 345 350

tca ttt acc gac tgg tca aag ttc ggc ttt tac caa gta gat ttt ggt 1104

Ser Phe Thr Asp Trp Ser Lys Phe Gly Phe Tyr Gln Val Asp Phe Gly

355 360 365

ttt ggt aaa cca gtt tgg acc ggc gtc cgt cca gaa cgt cca atc ttc 1152

Phe Gly Lys Pro Val Trp Thr Gly Val Arg Pro Glu Arg Pro Ile Phe

370 375 380

tca gcc gcg ata ttg atg agt aac agg gaa ggc gat ggc atc gag gct 1200

Ser Ala Ala Ile Leu Met Ser Asn Arg Glu Gly Asp Gly Ile Glu Ala

385 390 395 400

tgg ctt cat ttg gac aag aac gat atg cta ata ttt gag caa gac gaa 1248

Trp Leu His Leu Asp Lys Asn Asp Met Leu Ile Phe Glu Gln Asp Glu

405 410 415

gaa att aag ttg ttg ata acg taa 1272

Glu Ile Lys Leu Leu Ile Thr

420

<210> 23

<211> 423

<212> PRT

<213> CfACT1-8，针对其在酿酒酵母中的表达经密码子优化

<400> 23

Met Lys Val Glu Arg Ile Ser Arg Lys Phe Ile Lys Pro Tyr Thr Pro

1 5 10 15

Thr Pro Gln Asn Leu Lys Lys Tyr Lys Leu Ser Leu Leu Asp Lys Cys

20 25 30

Met Gly His Met Asp Phe Ala Val Val Leu Phe Tyr Glu Ser Lys Pro

35 40 45

Arg Asn Lys Asn Glu Leu Glu Glu Ser Leu Glu Lys Val Leu Val Asp

50 55 60

Phe Tyr Pro Leu Ala Gly Arg Tyr Thr Met Asn Asp His Ile Val Asp

65 70 75 80

Cys Ser Asp Glu Gly Ala Val Phe Val Glu Ala Glu Ala Pro Asn Val

85 90 95

Glu Leu Thr Val Asp Gln Leu Val Lys Asn Met Glu Ala Gln Thr Ile

100 105 110

His Asp Phe Leu Pro Asp Gln Tyr Phe Pro Ala Asp Ala Pro Asn Pro

115 120 125

Leu Leu Ser Ile Gln Val Thr His Phe Pro Cys Gly Gly Leu Ala Ile

130 135 140

Gly Ile Val Val Ser His Ala Val Phe Asp Gly Phe Ser Leu Gly Val

145 150 155 160

Phe Leu Ala Ala Trp Ser Lys Ala Thr Met Asn Pro Glu Arg Lys Ile

165 170 175

Glu Ile Thr Pro Ser Phe Asp Leu Pro Ser Leu Leu Pro Tyr Lys Asp

180 185 190

Glu Ser Phe Gly Leu Asn Phe Ser Glu Ile Val Lys Ala Glu Asn Ile

195 200 205

Val Val Lys Arg Leu Asn Phe Gly Lys Glu Ala Ile Thr Arg Leu Arg

210 215 220

Ser Lys Leu Ser Pro Asn Gln Asn Gly Lys Thr Ile Ser Arg Val Arg

225 230 235 240

Val Val Cys Ala Val Ile Val Lys Ala Leu Met Gly Leu Glu Arg Ala

245 250 255

Lys Thr Arg Asp Phe Met Ile Cys Gln Gly Ile Asn Met Arg Glu Arg

260 265 270

Thr Lys Ala Pro Leu Gln Lys His Ala Cys Gly Asn Leu Ala Val Ser

275 280 285

Ser Tyr Thr Arg Arg Val Ala Ala Ala Glu Ala Glu Glu Leu Gln Ser

290 295 300

Leu Val Asn Leu Ile Gly Asp Ser Ile Glu Lys Ser Ile Ala Asp Tyr

305 310 315 320

Ala Asp Ile Leu Ser Ser Asp Gln Asp Gly Arg His Ile Ile Ser Thr

325 330 335

Met Met Lys Ser Phe Met Gln Phe Ala Ala Pro Asp Ile Lys Ala Ile

340 345 350

Ser Phe Thr Asp Trp Ser Lys Phe Gly Phe Tyr Gln Val Asp Phe Gly

355 360 365

Phe Gly Lys Pro Val Trp Thr Gly Val Arg Pro Glu Arg Pro Ile Phe

370 375 380

Ser Ala Ala Ile Leu Met Ser Asn Arg Glu Gly Asp Gly Ile Glu Ala

385 390 395 400

Trp Leu His Leu Asp Lys Asn Asp Met Leu Ile Phe Glu Gln Asp Glu

405 410 415

Glu Ile Lys Leu Leu Ile Thr

420

<210> 24

<211> 654

<212> DNA

<213> OAH94415.1，针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(654)

<400> 24

atg gat acc aag agg gtc ggt tat aca gtc gtt gat ttg agt cag tgg 48

Met Asp Thr Lys Arg Val Gly Tyr Thr Val Val Asp Leu Ser Gln Trp

1 5 10 15

ggc aga aaa gaa cac ttc gaa gca ttc cag agc ttc gcc cag tgc acc 96

Gly Arg Lys Glu His Phe Glu Ala Phe Gln Ser Phe Ala Gln Cys Thr

20 25 30

ttt tcc cag act gtt caa tta gac ata act tcc ctg ctg aag act gtc 144

Phe Ser Gln Thr Val Gln Leu Asp Ile Thr Ser Leu Leu Lys Thr Val

35 40 45

aag cag aac gga tat aaa ttc tac ccg acc ttt atc tac ata ata agc 192

Lys Gln Asn Gly Tyr Lys Phe Tyr Pro Thr Phe Ile Tyr Ile Ile Ser

50 55 60

cgt tta gtg aac aag cat gcc gaa ttc cgt atg gcc atg aag gat ggt 240

Arg Leu Val Asn Lys His Ala Glu Phe Arg Met Ala Met Lys Asp Gly

65 70 75 80

gaa ctg gta att tgg gac tcc gta aac cct ggt tac aca atc ttt cac 288

Glu Leu Val Ile Trp Asp Ser Val Asn Pro Gly Tyr Thr Ile Phe His

85 90 95

gaa cag acc gaa aca ttt tca tct ctg tgg agc tat tac cac aag gac 336

Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Tyr Tyr His Lys Asp

100 105 110

atc aat cag ttt ctt aaa acg tat tct gaa gac atc gct cag tac ggg 384

Ile Asn Gln Phe Leu Lys Thr Tyr Ser Glu Asp Ile Ala Gln Tyr Gly

115 120 125

gac gat ctg gcg tac ttc ccc aag gag ttt att gaa aat atg ttc ttc 432

Asp Asp Leu Ala Tyr Phe Pro Lys Glu Phe Ile Glu Asn Met Phe Phe

130 135 140

gtg tca gcg aac cca tgg gtg agc ttc aca agt ttc aac tta aac gtg 480

Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asn Leu Asn Val

145 150 155 160

gcc aat att aat aac ttc ttc gca ccg gtc ttt aca atc ggt aaa tac 528

Ala Asn Ile Asn Asn Phe Phe Ala Pro Val Phe Thr Ile Gly Lys Tyr

165 170 175

tac acg cag ggc gac aag gtg ttg atg ccg tta gct att cag gtc cat 576

Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His

180 185 190

cat gca gtc tgc gac ggg ttt cat gtc ggc agg tta ttg aac gaa att 624

His Ala Val Cys Asp Gly Phe His Val Gly Arg Leu Leu Asn Glu Ile

195 200 205

cag cag tac tgc gat gag gga tgc aag taa 654

Gln Gln Tyr Cys Asp Glu Gly Cys Lys

210 215

<210> 25

<211> 217

<212> PRT

<213> OAH94415.1，针对其在酿酒酵母中的表达经密码子优化

<400> 25

Met Asp Thr Lys Arg Val Gly Tyr Thr Val Val Asp Leu Ser Gln Trp

1 5 10 15

Gly Arg Lys Glu His Phe Glu Ala Phe Gln Ser Phe Ala Gln Cys Thr

20 25 30

Phe Ser Gln Thr Val Gln Leu Asp Ile Thr Ser Leu Leu Lys Thr Val

35 40 45

Lys Gln Asn Gly Tyr Lys Phe Tyr Pro Thr Phe Ile Tyr Ile Ile Ser

50 55 60

Arg Leu Val Asn Lys His Ala Glu Phe Arg Met Ala Met Lys Asp Gly

65 70 75 80

Glu Leu Val Ile Trp Asp Ser Val Asn Pro Gly Tyr Thr Ile Phe His

85 90 95

Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Tyr Tyr His Lys Asp

100 105 110

Ile Asn Gln Phe Leu Lys Thr Tyr Ser Glu Asp Ile Ala Gln Tyr Gly

115 120 125

Asp Asp Leu Ala Tyr Phe Pro Lys Glu Phe Ile Glu Asn Met Phe Phe

130 135 140

Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asn Leu Asn Val

145 150 155 160

Ala Asn Ile Asn Asn Phe Phe Ala Pro Val Phe Thr Ile Gly Lys Tyr

165 170 175

Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His

180 185 190

His Ala Val Cys Asp Gly Phe His Val Gly Arg Leu Leu Asn Glu Ile

195 200 205

Gln Gln Tyr Cys Asp Glu Gly Cys Lys

210 215

<210> 26

<211> 1755

<212> DNA

<213> DfHAD_香鳞毛蕨(Dryopteris fragrans)

<220>

<221> CDS

<222> (1)..(1755)

<400> 26

atg gag ttc tct gcc tct gct cct cct cct agg cta gcc agt gtc ata 48

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

ata ttg gag cct ctc ggc ttc ctc ctc aca cca cac tac tcc tct cag 96

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

ctt ccc aaa aag ctg ctc cgt cgc ctg ttg tgc act aga atc tgg cac 144

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

agg tat cag cga ggc cgc ctt cgc ctg cgt gac gct gct atg ctg ctc 192

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

gcc cag ctc cca ttc cta gct gtg tct gat cac ccc tgg gct ctg gac 240

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

aat ctc gca agc ctg ctc cgc ccc aca gct gtg cgt gcg gtg cca tgg 288

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

atg ctg ctg ctg ctc gac ttc cta cga gac gag ctc cat ctg aag gta 336

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

gtc tgc gcg acc aac tcc tcc cca gaa gag ctg caa gag ctg cgc cac 384

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

cag ttt ccg gcc ctc ttt gcc aag gtc gat gcc acc gtt tct tca ggc 432

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

gag gag ggc gtg ggc aag ccg tcc gtg cgc ttc ctg cag gct gcg ttg 480

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

gac aaa gcc ggt gtc cac gcg cag caa acc ttg tat ctt gac tct ttt 528

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

gac agc ttg gag acc atc atg gct gca cgc tct ctt ggc atg cat gca 576

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

cta tct gta gag cca tgc cac att gat gag ctc acc gcc agg gcc tct 624

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

tcc ggc cag cta aga gat gca cag ctt ata agg cgt att gtg tgc gcc 672

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

atg cac ggg cca gca gta tct gca gtt gtg tcg ggc agt atc aca tcg 720

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

tcc ggc cca cag aca gca aag atc gag gaa ttg cca aca gct gct gat 768

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

agt cat ctc cgc agc gca gct ctc act tct gct cag cag ttt ttc ctc 816

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

aaa gtt att gct cca cat cgt cct gag aag cca ttc gtc cag ctt cca 864

Lys Val Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

tct ctc acc tcg gag ggc atc cga ata tac gac acc ttt gca cag ttt 912

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

gtc ata gcc gac ctg ctc gac gac acc cgc ttc cta ccc atg caa tct 960

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

cct cct ccc aat ggg ctc atc acc ttt gtt aac cca agc gcg tac ctt 1008

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

gct gat gat ata aag aat ggc aac agc cat att gtc ccg ggt gtg caa 1056

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

ttt tac gca tcc gat gcg tgc act ctc atc gac atc cca cat gac cta 1104

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

gac acc acc tcc gtt ggc ttg tca gta ctg cac aag ttt gga aag gtg 1152

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

gac aag gac aca ctc aac aaa gtg cta gac aga atg ctc gag caa gtg 1200

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

agt gaa gac gac ggc att ctg cag gtg tat ttt gat gtg gag cgt ccg 1248

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

cgc atc gat cca gtt gtg gtg gca aac acg gtg ttt ctg ttc cac ttg 1296

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

gga aag aga ggg cat gag gtg gcg agg agt gag aag ttt gtg gag agt 1344

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

gtg ctg ctg cag agg gca tac gaa gaa ggg acg ttg tat tac aac ctg 1392

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

ggg gaa gca ttt ttg gtg agt gtg gcg agg ctg gtg cac gag ttt aag 1440

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

gag cac ttt aca agg agc ggc atg agg agg gca ctg gag gag agg cta 1488

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

aga gag cgg gca agg gcg ggc atg caa gag agg gat gat gcg ctg gcg 1536

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

cta gcc atg cgc att cgt gca tgc gct ttg tgt ggc ctg gcc gga gag 1584

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

ggc ctc aca aaa gca gca gag cag gag ctt ttg cgc ctg cag tgc aag 1632

Gly Leu Thr Lys Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

tcc aag ggc tgt tgg ggg tgc cac cct ttc tat cgc aat ggc agt aat 1680

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

gtg ctc agc tgg atc ggc agt gag gcc ctt acc act gct tac gct att 1728

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

gct gcg cta cag ccc att gat att taa 1755

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 27

<211> 584

<212> PRT

<213> DfHAD_香鳞毛蕨(Dryopteris fragrans)

<400> 27

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

Lys Val Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

Gly Leu Thr Lys Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 28

<211> 1755

<212> DNA

<213> DfHAD-8(K532R)_香鳞毛蕨(Dryopteris fragrans)

<220>

<221> CDS

<222> (1)..(1755)

<400> 28

atg gag ttc tct gcc tct gct cct cct cct agg cta gcc agt gtc ata 48

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

ata ttg gag cct ctc ggc ttc ctc ctc aca cca cac tac tcc tct cag 96

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

ctt ccc aaa aag ctg ctc cgt cgc ctg ttg tgc act aga atc tgg cac 144

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

agg tat cag cga ggc cgc ctt cgc ctg cgt gac gct gct atg ctg ctc 192

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

gcc cag ctc cca ttc cta gct gtg tct gat cac ccc tgg gct ctg gac 240

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

aat ctc gca agc ctg ctc cgc ccc aca gct gtg cgt gcg gtg cca tgg 288

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

atg ctg ctg ctg ctc gac ttc cta cga gac gag ctc cat ctg aag gta 336

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

gtc tgc gcg acc aac tcc tcc cca gaa gag ctg caa gag ctg cgc cac 384

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

cag ttt ccg gcc ctc ttt gcc aag gtc gat gcc acc gtt tct tca ggc 432

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

gag gag ggc gtg ggc aag ccg tcc gtg cgc ttc ctg cag gct gcg ttg 480

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

gac aaa gcc ggt gtc cac gcg cag caa acc ttg tat ctt gac tct ttt 528

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

gac agc ttg gag acc atc atg gct gca cgc tct ctt ggc atg cat gca 576

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

cta tct gta gag cca tgc cac att gat gag ctc acc gcc agg gcc tct 624

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

tcc ggc cag cta aga gat gca cag ctt ata agg cgt att gtg tgc gcc 672

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

atg cac ggg cca gca gta tct gca gtt gtg tcg ggc agt atc aca tcg 720

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

tcc ggc cca cag aca gca aag atc gag gaa ttg cca aca gct gct gat 768

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

agt cat ctc cgc agc gca gct ctc act tct gct cag cag ttt ttc ctc 816

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

aaa gtt att gct cca cat cgt cct gag aag cca ttc gtc cag ctt cca 864

Lys Val Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

tct ctc acc tcg gag ggc atc cga ata tac gac acc ttt gca cag ttt 912

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

gtc ata gcc gac ctg ctc gac gac acc cgc ttc cta ccc atg caa tct 960

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

cct cct ccc aat ggg ctc atc acc ttt gtt aac cca agc gcg tac ctt 1008

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

gct gat gat ata aag aat ggc aac agc cat att gtc ccg ggt gtg caa 1056

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

ttt tac gca tcc gat gcg tgc act ctc atc gac atc cca cat gac cta 1104

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

gac acc acc tcc gtt ggc ttg tca gta ctg cac aag ttt gga aag gtg 1152

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

gac aag gac aca ctc aac aaa gtg cta gac aga atg ctc gag caa gtg 1200

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

agt gaa gac gac ggc att ctg cag gtg tat ttt gat gtg gag cgt ccg 1248

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

cgc atc gat cca gtt gtg gtg gca aac acg gtg ttt ctg ttc cac ttg 1296

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

gga aag aga ggg cat gag gtg gcg agg agt gag aag ttt gtg gag agt 1344

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

gtg ctg ctg cag agg gca tac gaa gaa ggg acg ttg tat tac aac ctg 1392

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

ggg gaa gca ttt ttg gtg agt gtg gcg agg ctg gtg cac gag ttt aag 1440

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

gag cac ttt aca agg agc ggc atg agg agg gca ctg gag gag agg cta 1488

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

aga gag cgg gca agg gcg ggc atg caa gag agg gat gat gcg ctg gcg 1536

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

ctg gcc atg cgc att cgt gca tgc gct ttg tgt ggc ctg gcc gga gag 1584

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

ggc ctc aca aga gca gca gag cag gag cta ctg cgc ctg cag tgc aag 1632

Gly Leu Thr Arg Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

tcc aag ggc tgt tgg ggg tgc cac cct ttc tat cgc aat ggc agt aat 1680

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

gtg ctc agc tgg atc ggc agt gag gcc ctt acc act gct tac gct att 1728

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

gct gcg cta cag ccc att gat att taa 1755

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 29

<211> 584

<212> PRT

<213> DfHAD-8(K532R)_香鳞毛蕨(Dryopteris fragrans)

<400> 29

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

Lys Val Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

Gly Leu Thr Arg Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 30

<211> 1755

<212> DNA

<213> DfHAD-9(V274A)_香鳞毛蕨(Dryopteris fragrans)

<220>

<221> CDS

<222> (1)..(1755)

<400> 30

atg gag ttc tct gcc tct gct cct cct cct agg cta gcc agt gtc ata 48

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

ata ttg gag cct ctc ggc ttc ctc ctc aca cca cac tac tcc tct cag 96

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

ctt ccc aaa aag ctg ctc cgt cgc ctg ttg tgc act aga atc tgg cac 144

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

agg tat cag cga ggc cgc ctt cgc ctg cgt gac gct gct atg ctg ctc 192

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

gcc cag ctc cca ttc cta gct gtg tct gat cac ccc tgg gct ctg gac 240

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

aat ctc gca agc ctg ctc cgc ccc aca gct gtg cgt gcg gtg cca tgg 288

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

atg ctg ctg ctg ctc gac ttc cta cga gac gag ctc cat ctg aag gta 336

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

gtc tgc gcg acc aac tcc tcc cca gaa gag ctg caa gag ctg cgc cac 384

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

cag ttt ccg gcc ctc ttt gcc aag gtc gat gcc acc gtt tct tca ggc 432

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

gag gag ggc gtg ggc aag ccg tcc gtg cgc ttc ctg cag gct gcg ttg 480

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

gac aaa gcc ggt gtc cac gcg cag caa acc ttg tat ctt gac tct ttt 528

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

gac agc ttg gag acc atc atg gct gca cgc tct ctt ggc atg cat gca 576

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

cta tct gta gag cca tgc cac att gat gag ctc acc gcc agg gcc tct 624

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

tcc ggc cag cta aga gat gca cag ctt ata agg cgt att gtg tgc gcc 672

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

atg cac ggg cca gca gta tct gca gtt gtg tcg ggc agt atc aca tcg 720

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

tcc ggc cca cag aca gca aag atc gag gaa ttg cca aca gct gct gat 768

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

agt cat ctc cgc agc gca gct ctc act tct gct cag cag ttt ttc ctc 816

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

aaa gct att gct cca cat cgt cct gag aag cca ttc gtc cag ctt cca 864

Lys Ala Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

tct ctc acc tcg gag ggc atc cga ata tac gac acc ttt gca cag ttt 912

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

gtc ata gcc gac ctg ctc gac gac acc cgc ttc cta ccc atg caa tct 960

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

cct cct ccc aat ggg ctc atc acc ttt gtt aac cca agc gcg tac ctt 1008

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

gct gat gat ata aag aat ggc aac agc cat att gtc ccg ggt gtg caa 1056

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

ttt tac gca tct gat gcg tgc act ctc atc gac atc cca cat gac cta 1104

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

gac acc acc tcc gtt ggc ttg tca gta ctg cac aag ttt gga aag gtg 1152

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

gac aag gac aca ctc aac aaa gtg cta gac aga atg ctg gag caa gtg 1200

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

agt gaa gac gac ggc att ctc cag gtg tat ttt gat gtg gag cgt ccg 1248

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

cgc atc gat cca gtt gtg gtg gca aac acg gtg ttt ctg ttc cac ttg 1296

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

gga aag aga ggg cat gag gtg gcg agg agt gag aag ttt gtg gag agt 1344

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

gtg ctg ctg cag agg gca tac gaa gaa ggg acg ttg tat tac aac ctg 1392

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

ggg gaa gca ttt ttg gtg agt gtg gcg agg ctg gtg cac gag ttt aag 1440

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

gag cac ttt aca agg agc ggc atg agg agg gca ctg gag gag agg cta 1488

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

aga gag cgg gca agg gcg ggc atg caa gag agg gat gat gcg ctg gcg 1536

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

ctg gcc atg cgc att cgt gca tgc gct ttg tgt ggc ctg gcc gga gag 1584

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

ggc ctc aca aaa gca gca gag cag gag cta ctg cgc ctg cag tgc aag 1632

Gly Leu Thr Lys Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

tcc aag ggc tgt tgg ggg tgc cac cct ttc tat cgc aat ggc agt aat 1680

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

gtg ctc agc tgg atc ggc agt gag gcc ctt acc act gct tac gct att 1728

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

gct gcg cta cag ccc att gat att taa 1755

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 31

<211> 584

<212> PRT

<213> DfHAD-9(V274A)_香鳞毛蕨(Dryopteris fragrans)

<400> 31

Met Glu Phe Ser Ala Ser Ala Pro Pro Pro Arg Leu Ala Ser Val Ile

1 5 10 15

Ile Leu Glu Pro Leu Gly Phe Leu Leu Thr Pro His Tyr Ser Ser Gln

20 25 30

Leu Pro Lys Lys Leu Leu Arg Arg Leu Leu Cys Thr Arg Ile Trp His

35 40 45

Arg Tyr Gln Arg Gly Arg Leu Arg Leu Arg Asp Ala Ala Met Leu Leu

50 55 60

Ala Gln Leu Pro Phe Leu Ala Val Ser Asp His Pro Trp Ala Leu Asp

65 70 75 80

Asn Leu Ala Ser Leu Leu Arg Pro Thr Ala Val Arg Ala Val Pro Trp

85 90 95

Met Leu Leu Leu Leu Asp Phe Leu Arg Asp Glu Leu His Leu Lys Val

100 105 110

Val Cys Ala Thr Asn Ser Ser Pro Glu Glu Leu Gln Glu Leu Arg His

115 120 125

Gln Phe Pro Ala Leu Phe Ala Lys Val Asp Ala Thr Val Ser Ser Gly

130 135 140

Glu Glu Gly Val Gly Lys Pro Ser Val Arg Phe Leu Gln Ala Ala Leu

145 150 155 160

Asp Lys Ala Gly Val His Ala Gln Gln Thr Leu Tyr Leu Asp Ser Phe

165 170 175

Asp Ser Leu Glu Thr Ile Met Ala Ala Arg Ser Leu Gly Met His Ala

180 185 190

Leu Ser Val Glu Pro Cys His Ile Asp Glu Leu Thr Ala Arg Ala Ser

195 200 205

Ser Gly Gln Leu Arg Asp Ala Gln Leu Ile Arg Arg Ile Val Cys Ala

210 215 220

Met His Gly Pro Ala Val Ser Ala Val Val Ser Gly Ser Ile Thr Ser

225 230 235 240

Ser Gly Pro Gln Thr Ala Lys Ile Glu Glu Leu Pro Thr Ala Ala Asp

245 250 255

Ser His Leu Arg Ser Ala Ala Leu Thr Ser Ala Gln Gln Phe Phe Leu

260 265 270

Lys Ala Ile Ala Pro His Arg Pro Glu Lys Pro Phe Val Gln Leu Pro

275 280 285

Ser Leu Thr Ser Glu Gly Ile Arg Ile Tyr Asp Thr Phe Ala Gln Phe

290 295 300

Val Ile Ala Asp Leu Leu Asp Asp Thr Arg Phe Leu Pro Met Gln Ser

305 310 315 320

Pro Pro Pro Asn Gly Leu Ile Thr Phe Val Asn Pro Ser Ala Tyr Leu

325 330 335

Ala Asp Asp Ile Lys Asn Gly Asn Ser His Ile Val Pro Gly Val Gln

340 345 350

Phe Tyr Ala Ser Asp Ala Cys Thr Leu Ile Asp Ile Pro His Asp Leu

355 360 365

Asp Thr Thr Ser Val Gly Leu Ser Val Leu His Lys Phe Gly Lys Val

370 375 380

Asp Lys Asp Thr Leu Asn Lys Val Leu Asp Arg Met Leu Glu Gln Val

385 390 395 400

Ser Glu Asp Asp Gly Ile Leu Gln Val Tyr Phe Asp Val Glu Arg Pro

405 410 415

Arg Ile Asp Pro Val Val Val Ala Asn Thr Val Phe Leu Phe His Leu

420 425 430

Gly Lys Arg Gly His Glu Val Ala Arg Ser Glu Lys Phe Val Glu Ser

435 440 445

Val Leu Leu Gln Arg Ala Tyr Glu Glu Gly Thr Leu Tyr Tyr Asn Leu

450 455 460

Gly Glu Ala Phe Leu Val Ser Val Ala Arg Leu Val His Glu Phe Lys

465 470 475 480

Glu His Phe Thr Arg Ser Gly Met Arg Arg Ala Leu Glu Glu Arg Leu

485 490 495

Arg Glu Arg Ala Arg Ala Gly Met Gln Glu Arg Asp Asp Ala Leu Ala

500 505 510

Leu Ala Met Arg Ile Arg Ala Cys Ala Leu Cys Gly Leu Ala Gly Glu

515 520 525

Gly Leu Thr Lys Ala Ala Glu Gln Glu Leu Leu Arg Leu Gln Cys Lys

530 535 540

Ser Lys Gly Cys Trp Gly Cys His Pro Phe Tyr Arg Asn Gly Ser Asn

545 550 555 560

Val Leu Ser Trp Ile Gly Ser Glu Ala Leu Thr Thr Ala Tyr Ala Ile

565 570 575

Ala Ala Leu Gln Pro Ile Asp Ile

580

<210> 32

<211> 1755

<212> DNA

<213> 通过大肠杆菌Genscript遗传密码子频率对DfHAD进行密码子优化的序列

<400> 32

atggagttca gcgcgagcgc tccgccgccg cgtctggcga gcgtgatcat tctggaaccg 60

ctgggttttc tgctgacccc gcactacagc agccagctgc cgaagaaact gctgcgtcgt 120

ctgctgtgca cccgtatctg gcaccgttat cagcgtggcc gtctgcgtct gcgtgacgcg 180

gcgatgctgc tggcgcaact gccgttcctg gcggttagcg accacccgtg ggcgctggat 240

aacctggcga gcctgctgcg tccgaccgcg gttcgtgcgg tgccgtggat gctgctgctg 300

ctggactttc tgcgtgatga gctgcacctg aaagtggttt gcgcgaccaa cagcagcccg 360

gaggaactgc aggaactgcg tcaccaattc ccggcgctgt ttgcgaaggt tgacgcgacc 420

gtgagcagcg gcgaggaagg tgttggcaaa ccgagcgtgc gtttcctgca agcggcgctg 480

gataaggcgg gcgtgcacgc gcagcaaacc ctgtacctgg acagctttga tagcctggag 540

accatcatgg cggcgcgtag cctgggtatg cacgcgctga gcgttgagcc gtgccacatt 600

gacgaactga ccgcgcgtgc gagcagcggt cagctgcgtg atgcgcaact gatccgtcgt 660

attgtttgcg cgatgcacgg tccggctgtg agcgcggtgg ttagcggtag catcaccagc 720

agcggtccgc agaccgcgaa aattgaggaa ctgccgaccg cggcggacag ccacctgcgt 780

agcgcggcgc tgaccagcgc gcagcaattc tttctgaaag tgattgcgcc gcaccgtccg 840

gagaagccgt tcgttcaact gccgagcctg accagcgaag gtatccgtat ttatgacacc 900

ttcgcgcagt ttgtgatcgc ggatctgctg gacgataccc gtttcctgcc gatgcaaagc 960

ccgccgccga acggcctgat tacctttgtt aacccgagcg cgtacctggc ggacgatatc 1020

aaaaacggta acagccacat tgttccgggc gtgcagttct atgcgagcga cgcgtgcacc 1080

ctgatcgata ttccgcacga cctggatacc accagcgttg gtctgagcgt gctgcacaag 1140

tttggcaaag ttgacaagga taccctgaac aaggtgctgg atcgtatgct ggagcaagtt 1200

agcgaagacg atggtatcct gcaagtttac tttgacgtgg agcgtccgcg tattgatccg 1260

gtggttgtgg cgaacaccgt gttcctgttt cacctgggta aacgtggcca cgaagttgcg 1320

cgtagcgaga agttcgttga aagcgtgctg ctgcagcgtg cgtacgagga aggcaccctg 1380

tactataacc tgggcgaagc gtttctggtt agcgtggcgc gtctggtgca cgagttcaaa 1440

gaacacttta cccgtagcgg tatgcgtcgt gcgctggagg aacgtctgcg tgagcgtgcg 1500

cgtgcgggta tgcaagaacg tgacgatgcg ctggcgctgg cgatgcgtat ccgtgcgtgc 1560

gcgctgtgcg gtctggcggg cgagggtctg accaaggcgg cggagcagga actgctgcgt 1620

ctgcaatgca agagcaaagg ttgctggggc tgccacccgt tctaccgtaa cggtagcaac 1680

gttctgagct ggatcggcag cgaagcgctg accaccgcgt atgcgattgc ggcgctgcag 1740

ccgatcgaca tttaa 1755

<210> 33

<211> 1755

<212> DNA

<213> 通过烟草Genscript遗传密码子频率对DfHAD进行密码子优化的序列

<400> 33

atggaatttt ctgcttcagc tccacctcca agacttgctt cagttattat tcttgagcct 60

ttgggatttc ttttgactcc acattactct tcacaattgc ctaagaaact tttgagaagg 120

cttttgtgta caagaatttg gcataggtac caaaggggta ggcttagatt gagggatgct 180

gctatgcttt tggctcaact tccatttttg gctgtttcag atcatccttg ggctcttgat 240

aatttggctt ctcttttgag accaactgct gttagggctg ttccttggat gcttttgctt 300

ttggattttc ttagagatga acttcatttg aaggttgttt gcgctactaa ttcttcacca 360

gaagagcttc aagagttgag gcatcaattt cctgctttgt ttgctaaggt tgatgctaca 420

gtttcttcag gagaagaggg agttggtaaa ccatctgtta gatttcttca agctgctttg 480

gataaggctg gtgttcatgc tcaacaaact ctttatttgg attctttcga ttcacttgaa 540

acaattatgg ctgctaggtc attgggaatg catgctcttt ctgttgaacc atgtcatatt 600

gatgagttga ctgctagagc ttcttcagga caattgaggg atgctcaact tattagaagg 660

attgtttgcg ctatgcatgg tcctgctgtt tcagctgttg tttctggatc aattacttct 720

tcaggtccac aaacagctaa aattgaagag cttcctactg ctgctgattc tcatttgaga 780

tcagctgctc ttacatctgc tcaacaattt ttccttaaag ttattgctcc acatagacct 840

gaaaagccat ttgttcaact tccttctttg acttcagagg gaatcaggat ctatgataca 900

ttcgctcaat tcgttatcgc tgatcttttg gatgatacta ggtttttgcc aatgcaatca 960

cctccaccta atggtcttat cacattcgtt aacccttctg cttatttggc tgatgatatt 1020

aaaaatggta actcacatat tgttccaggt gttcaatttt acgcttctga tgcttgtact 1080

ttgattgata ttcctcatga tcttgatact acatctgttg gactttcagt tttgcataag 1140

ttcggtaaag ttgataagga tacacttaat aaggttttgg atagaatgct tgaacaagtt 1200

tcagaggatg atggaatcct tcaagtttac ttcgatgttg aaagacctag gattgatcca 1260

gttgttgttg ctaacactgt ttttcttttc catttgggaa aaagaggtca tgaggttgct 1320

agatcagaaa agtttgttga gtctgttctt ttgcaaagag cttacgaaga gggaactttg 1380

tattacaatc ttggtgaagc ttttcttgtt tctgttgcta gacttgttca tgagtttaag 1440

gagcatttta caaggtctgg aatgagaagg gctttggaag agagacttag ggaaagagct 1500

agggctggta tgcaagagag agatgatgct cttgctttgg ctatgagaat tagggcttgt 1560

gctctttgcg gtttggctgg agaaggtctt acaaaggctg ctgaacaaga gcttttgaga 1620

ttgcaatgca agtctaaagg atgttggggt tgccatccat tctacaggaa tggttctaac 1680

gttttgtcat ggattggttc tgaggctctt actacagctt acgctattgc tgctcttcaa 1740

cctattgata tttga 1755

<210> 34

<211> 1755

<212> DNA

<213> 通过大肠杆菌Genscript遗传密码子频率对DfHAD-8(K532R)进行密码子优化的序列

<400> 34

atggagttca gcgcgagcgc tccgccgccg cgtctggcga gcgtgatcat tctggaaccg 60

ctgggttttc tgctgacccc gcactacagc agccagctgc cgaagaaact gctgcgtcgt 120

ctgctgtgca cccgtatctg gcaccgttat cagcgtggcc gtctgcgtct gcgtgacgcg 180

gcgatgctgc tggcgcaact gccgttcctg gcggttagcg accacccgtg ggcgctggat 240

aacctggcga gcctgctgcg tccgaccgcg gttcgtgcgg tgccgtggat gctgctgctg 300

ctggactttc tgcgtgatga gctgcacctg aaagtggttt gcgcgaccaa cagcagcccg 360

gaggaactgc aggaactgcg tcaccaattc ccggcgctgt ttgcgaaggt tgacgcgacc 420

gtgagcagcg gcgaggaagg tgttggcaaa ccgagcgtgc gtttcctgca agcggcgctg 480

gataaggcgg gcgtgcacgc gcagcaaacc ctgtacctgg acagctttga tagcctggag 540

accatcatgg cggcgcgtag cctgggtatg cacgcgctga gcgttgagcc gtgccacatt 600

gacgaactga ccgcgcgtgc gagcagcggt cagctgcgtg atgcgcaact gatccgtcgt 660

attgtttgcg cgatgcacgg tccggctgtg agcgcggtgg ttagcggtag catcaccagc 720

agcggtccgc agaccgcgaa aattgaggaa ctgccgaccg cggcggacag ccacctgcgt 780

agcgcggcgc tgaccagcgc gcagcaattc tttctgaaag tgattgcgcc gcaccgtccg 840

gagaagccgt tcgttcaact gccgagcctg accagcgaag gtatccgtat ttatgacacc 900

ttcgcgcagt ttgtgatcgc ggatctgctg gacgataccc gtttcctgcc gatgcaaagc 960

ccgccgccga acggcctgat tacctttgtt aacccgagcg cgtacctggc ggacgatatc 1020

aaaaacggta acagccacat tgttccgggc gtgcagttct atgcgagcga cgcgtgcacc 1080

ctgatcgata ttccgcacga cctggatacc accagcgttg gtctgagcgt gctgcacaag 1140

tttggcaaag ttgacaagga taccctgaac aaggtgctgg atcgtatgct ggagcaagtt 1200

agcgaagacg atggtatcct gcaagtttac tttgacgtgg agcgtccgcg tattgatccg 1260

gtggttgtgg cgaacaccgt gttcctgttt cacctgggta aacgtggcca cgaagttgcg 1320

cgtagcgaga agttcgttga aagcgtgctg ctgcagcgtg cgtacgagga aggcaccctg 1380

tactataacc tgggcgaagc gtttctggtt agcgtggcgc gtctggtgca cgagttcaaa 1440

gaacacttta cccgtagcgg tatgcgtcgt gcgctggagg aacgtctgcg tgagcgtgcg 1500

cgtgcgggta tgcaagaacg tgacgatgcg ctggcgctgg cgatgcgtat ccgtgcgtgc 1560

gcgctgtgcg gtctggcggg cgagggtctg acccgggcgg cggagcagga actgctgcgt 1620

ctgcaatgca agagcaaagg ttgctggggc tgccacccgt tctaccgtaa cggtagcaac 1680

gttctgagct ggatcggcag cgaagcgctg accaccgcgt atgcgattgc ggcgctgcag 1740

ccgatcgaca tttaa 1755

<210> 35

<211> 1755

<212> DNA

<213> 通过大肠杆菌Genscript遗传密码子频率对DfHAD-9(V274A)进行密码子优化的序列

<400> 35

atggagttca gcgcgagcgc tccgccgccg cgtctggcga gcgtgatcat tctggaaccg 60

ctgggttttc tgctgacccc gcactacagc agccagctgc cgaagaaact gctgcgtcgt 120

ctgctgtgca cccgtatctg gcaccgttat cagcgtggcc gtctgcgtct gcgtgacgcg 180

gcgatgctgc tggcgcaact gccgttcctg gcggttagcg accacccgtg ggcgctggat 240

aacctggcga gcctgctgcg tccgaccgcg gttcgtgcgg tgccgtggat gctgctgctg 300

ctggactttc tgcgtgatga gctgcacctg aaagtggttt gcgcgaccaa cagcagcccg 360

gaggaactgc aggaactgcg tcaccaattc ccggcgctgt ttgcgaaggt tgacgcgacc 420

gtgagcagcg gcgaggaagg tgttggcaaa ccgagcgtgc gtttcctgca agcggcgctg 480

gataaggcgg gcgtgcacgc gcagcaaacc ctgtacctgg acagctttga tagcctggag 540

accatcatgg cggcgcgtag cctgggtatg cacgcgctga gcgttgagcc gtgccacatt 600

gacgaactga ccgcgcgtgc gagcagcggt cagctgcgtg atgcgcaact gatccgtcgt 660

attgtttgcg cgatgcacgg tccggctgtg agcgcggtgg ttagcggtag catcaccagc 720

agcggtccgc agaccgcgaa aattgaggaa ctgccgaccg cggcggacag ccacctgcgt 780

agcgcggcgc tgaccagcgc gcagcaattc tttctgaaag cgattgcgcc gcaccgtccg 840

gagaagccgt tcgttcaact gccgagcctg accagcgaag gtatccgtat ttatgacacc 900

ttcgcgcagt ttgtgatcgc ggatctgctg gacgataccc gtttcctgcc gatgcaaagc 960

ccgccgccga acggcctgat tacctttgtt aacccgagcg cgtacctggc ggacgatatc 1020

aaaaacggta acagccacat tgttccgggc gtgcagttct atgcgagcga cgcgtgcacc 1080

ctgatcgata ttccgcacga cctggatacc accagcgttg gtctgagcgt gctgcacaag 1140

tttggcaaag ttgacaagga taccctgaac aaggtgctgg atcgtatgct ggagcaagtt 1200

agcgaagacg atggtatcct gcaagtttac tttgacgtgg agcgtccgcg tattgatccg 1260

gtggttgtgg cgaacaccgt gttcctgttt cacctgggta aacgtggcca cgaagttgcg 1320

cgtagcgaga agttcgttga aagcgtgctg ctgcagcgtg cgtacgagga aggcaccctg 1380

tactataacc tgggcgaagc gtttctggtt agcgtggcgc gtctggtgca cgagttcaaa 1440

gaacacttta cccgtagcgg tatgcgtcgt gcgctggagg aacgtctgcg tgagcgtgcg 1500

cgtgcgggta tgcaagaacg tgacgatgcg ctggcgctgg cgatgcgtat ccgtgcgtgc 1560

gcgctgtgcg gtctggcggg cgagggtctg accaaggcgg cggagcagga actgctgcgt 1620

ctgcaatgca agagcaaagg ttgctggggc tgccacccgt tctaccgtaa cggtagcaac 1680

gttctgagct ggatcggcag cgaagcgctg accaccgcgt atgcgattgc ggcgctgcag 1740

ccgatcgaca tttaa 1755

<210> 36

<211> 2496

<212> DNA

<213> 通过大肠杆菌Genscript遗传密码子频率对DfHAD-6His-GST进行密码子优化的序列

<220>

<221> CDS

<222> (1)..(2496)

<400> 36

atg tct ggt tct cat cat cat cat cat cat agc agc ggt atg tcc cct 48

Met Ser Gly Ser His His His His His His Ser Ser Gly Met Ser Pro

1 5 10 15

ata cta ggt tat tgg aaa att aag ggc ctt gtg caa ccc act cga ctt 96

Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro Thr Arg Leu

20 25 30

ctt ttg gaa tat ctt gaa gaa aaa tat gaa gag cat ttg tat gag cgc 144

Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu Tyr Glu Arg

35 40 45

gat gaa ggt gat aaa tgg cga aac aaa aag ttt gaa ttg ggt ttg gag 192

Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu Gly Leu Glu

50 55 60

ttt ccc aat ctt cct tat tat att gat ggt gat gtt aaa tta aca cag 240

Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys Leu Thr Gln

65 70 75 80

tct atg gcc atc ata cgt tat ata gct gac aag cac aac atg ttg ggt 288

Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn Met Leu Gly

85 90 95

ggt tgt cca aaa gag cgt gca gag att tca atg ctt gaa gga gcg gtt 336

Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu Gly Ala Val

100 105 110

ttg gat att aga tac ggt gtt tcg aga att gca tat agt aaa gac ttt 384

Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser Lys Asp Phe

115 120 125

gaa act ctc aaa gtt gat ttt ctt agc aag cta cct gaa atg ctg aaa 432

Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu Met Leu Lys

130 135 140

atg ttc gaa gat cgt tta tgt cat aaa aca tat tta aat ggt gat cat 480

Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn Gly Asp His

145 150 155 160

gta acc cat cct gac ttc atg ttg tat gac gct ctt gat gtt gtt tta 528

Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp Val Val Leu

165 170 175

tac atg gac cca atg tgc ctg gat gcg ttc cca aaa tta gtt tgt ttt 576

Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu Val Cys Phe

180 185 190

aaa aaa cgt att gaa gct atc cca caa att gat aag tac ttg aaa tcc 624

Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr Leu Lys Ser

195 200 205

agc aag tat ata gca tgg cct ttg cag ggc tgg caa gcc acg ttt ggt 672

Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala Thr Phe Gly

210 215 220

ggt ggc gac cat cct cca aaa tcg gat ctg ggc cac aca ggc cat aga 720

Gly Gly Asp His Pro Pro Lys Ser Asp Leu Gly His Thr Gly His Arg

225 230 235 240

tct gac gac gac gac aag cat atg gag ttc agc gcg agc gct ccg ccg 768

Ser Asp Asp Asp Asp Lys His Met Glu Phe Ser Ala Ser Ala Pro Pro

245 250 255

ccg cgt ctg gcg agc gtg atc att ctg gaa ccg ctg ggt ttt ctg ctg 816

Pro Arg Leu Ala Ser Val Ile Ile Leu Glu Pro Leu Gly Phe Leu Leu

260 265 270

acc ccg cac tac agc agc cag ctg ccg aag aaa ctg ctg cgt cgt ctg 864

Thr Pro His Tyr Ser Ser Gln Leu Pro Lys Lys Leu Leu Arg Arg Leu

275 280 285

ctg tgc acc cgt atc tgg cac cgt tat cag cgt ggc cgt ctg cgt ctg 912

Leu Cys Thr Arg Ile Trp His Arg Tyr Gln Arg Gly Arg Leu Arg Leu

290 295 300

cgt gac gcg gcg atg ctg ctg gcg caa ctg ccg ttc ctg gcg gtt agc 960

Arg Asp Ala Ala Met Leu Leu Ala Gln Leu Pro Phe Leu Ala Val Ser

305 310 315 320

gac cac ccg tgg gcg ctg gat aac ctg gcg agc ctg ctg cgt ccg acc 1008

Asp His Pro Trp Ala Leu Asp Asn Leu Ala Ser Leu Leu Arg Pro Thr

325 330 335

gcg gtt cgt gcg gtg ccg tgg atg ctg ctg ctg ctg gac ttt ctg cgt 1056

Ala Val Arg Ala Val Pro Trp Met Leu Leu Leu Leu Asp Phe Leu Arg

340 345 350

gat gag ctg cac ctg aaa gtg gtt tgc gcg acc aac agc agc ccg gag 1104

Asp Glu Leu His Leu Lys Val Val Cys Ala Thr Asn Ser Ser Pro Glu

355 360 365

gaa ctg cag gaa ctg cgt cac caa ttc ccg gcg ctg ttt gcg aag gtt 1152

Glu Leu Gln Glu Leu Arg His Gln Phe Pro Ala Leu Phe Ala Lys Val

370 375 380

gac gcg acc gtg agc agc ggc gag gaa ggt gtt ggc aaa ccg agc gtg 1200

Asp Ala Thr Val Ser Ser Gly Glu Glu Gly Val Gly Lys Pro Ser Val

385 390 395 400

cgt ttc ctg caa gcg gcg ctg gat aag gcg ggc gtg cac gcg cag caa 1248

Arg Phe Leu Gln Ala Ala Leu Asp Lys Ala Gly Val His Ala Gln Gln

405 410 415

acc ctg tac ctg gac agc ttt gat agc ctg gag acc atc atg gcg gcg 1296

Thr Leu Tyr Leu Asp Ser Phe Asp Ser Leu Glu Thr Ile Met Ala Ala

420 425 430

cgt agc ctg ggt atg cac gcg ctg agc gtt gag ccg tgc cac att gac 1344

Arg Ser Leu Gly Met His Ala Leu Ser Val Glu Pro Cys His Ile Asp

435 440 445

gaa ctg acc gcg cgt gcg agc agc ggt cag ctg cgt gat gcg caa ctg 1392

Glu Leu Thr Ala Arg Ala Ser Ser Gly Gln Leu Arg Asp Ala Gln Leu

450 455 460

atc cgt cgt att gtt tgc gcg atg cac ggt ccg gct gtg agc gcg gtg 1440

Ile Arg Arg Ile Val Cys Ala Met His Gly Pro Ala Val Ser Ala Val

465 470 475 480

gtt agc ggt agc atc acc agc agc ggt ccg cag acc gcg aaa att gag 1488

Val Ser Gly Ser Ile Thr Ser Ser Gly Pro Gln Thr Ala Lys Ile Glu

485 490 495

gaa ctg ccg acc gcg gcg gac agc cac ctg cgt agc gcg gcg ctg acc 1536

Glu Leu Pro Thr Ala Ala Asp Ser His Leu Arg Ser Ala Ala Leu Thr

500 505 510

agc gcg cag caa ttc ttt ctg aaa gtg att gcg ccg cac cgt ccg gag 1584

Ser Ala Gln Gln Phe Phe Leu Lys Val Ile Ala Pro His Arg Pro Glu

515 520 525

aag ccg ttc gtt caa ctg ccg agc ctg acc agc gaa ggt atc cgt att 1632

Lys Pro Phe Val Gln Leu Pro Ser Leu Thr Ser Glu Gly Ile Arg Ile

530 535 540

tat gac acc ttc gcg cag ttt gtg atc gcg gat ctg ctg gac gat acc 1680

Tyr Asp Thr Phe Ala Gln Phe Val Ile Ala Asp Leu Leu Asp Asp Thr

545 550 555 560

cgt ttc ctg ccg atg caa agc ccg ccg ccg aac ggc ctg att acc ttt 1728

Arg Phe Leu Pro Met Gln Ser Pro Pro Pro Asn Gly Leu Ile Thr Phe

565 570 575

gtt aac ccg agc gcg tac ctg gcg gac gat atc aaa aac ggt aac agc 1776

Val Asn Pro Ser Ala Tyr Leu Ala Asp Asp Ile Lys Asn Gly Asn Ser

580 585 590

cac att gtt ccg ggc gtg cag ttc tat gcg agc gac gcg tgc acc ctg 1824

His Ile Val Pro Gly Val Gln Phe Tyr Ala Ser Asp Ala Cys Thr Leu

595 600 605

atc gat att ccg cac gac ctg gat acc acc agc gtt ggt ctg agc gtg 1872

Ile Asp Ile Pro His Asp Leu Asp Thr Thr Ser Val Gly Leu Ser Val

610 615 620

ctg cac aag ttt ggc aaa gtt gac aag gat acc ctg aac aag gtg ctg 1920

Leu His Lys Phe Gly Lys Val Asp Lys Asp Thr Leu Asn Lys Val Leu

625 630 635 640

gat cgt atg ctg gag caa gtt agc gaa gac gat ggt atc ctg caa gtt 1968

Asp Arg Met Leu Glu Gln Val Ser Glu Asp Asp Gly Ile Leu Gln Val

645 650 655

tac ttt gac gtg gag cgt ccg cgt att gat ccg gtg gtt gtg gcg aac 2016

Tyr Phe Asp Val Glu Arg Pro Arg Ile Asp Pro Val Val Val Ala Asn

660 665 670

acc gtg ttc ctg ttt cac ctg ggt aaa cgt ggc cac gaa gtt gcg cgt 2064

Thr Val Phe Leu Phe His Leu Gly Lys Arg Gly His Glu Val Ala Arg

675 680 685

agc gag aag ttc gtt gaa agc gtg ctg ctg cag cgt gcg tac gag gaa 2112

Ser Glu Lys Phe Val Glu Ser Val Leu Leu Gln Arg Ala Tyr Glu Glu

690 695 700

ggc acc ctg tac tat aac ctg ggc gaa gcg ttt ctg gtt agc gtg gcg 2160

Gly Thr Leu Tyr Tyr Asn Leu Gly Glu Ala Phe Leu Val Ser Val Ala

705 710 715 720

cgt ctg gtg cac gag ttc aaa gaa cac ttt acc cgt agc ggt atg cgt 2208

Arg Leu Val His Glu Phe Lys Glu His Phe Thr Arg Ser Gly Met Arg

725 730 735

cgt gcg ctg gag gaa cgt ctg cgt gag cgt gcg cgt gcg ggt atg caa 2256

Arg Ala Leu Glu Glu Arg Leu Arg Glu Arg Ala Arg Ala Gly Met Gln

740 745 750

gaa cgt gac gat gcg ctg gcg ctg gcg atg cgt atc cgt gcg tgc gcg 2304

Glu Arg Asp Asp Ala Leu Ala Leu Ala Met Arg Ile Arg Ala Cys Ala

755 760 765

ctg tgc ggt ctg gcg ggc gag ggt ctg acc aag gcg gcg gag cag gaa 2352

Leu Cys Gly Leu Ala Gly Glu Gly Leu Thr Lys Ala Ala Glu Gln Glu

770 775 780

ctg ctg cgt ctg caa tgc aag agc aaa ggt tgc tgg ggc tgc cac ccg 2400

Leu Leu Arg Leu Gln Cys Lys Ser Lys Gly Cys Trp Gly Cys His Pro

785 790 795 800

ttc tac cgt aac ggt agc aac gtt ctg agc tgg atc ggc agc gaa gcg 2448

Phe Tyr Arg Asn Gly Ser Asn Val Leu Ser Trp Ile Gly Ser Glu Ala

805 810 815

ctg acc acc gcg tat gcg att gcg gcg ctg cag ccg atc gac att taa 2496

Leu Thr Thr Ala Tyr Ala Ile Ala Ala Leu Gln Pro Ile Asp Ile

820 825 830

<210> 37

<211> 831

<212> PRT

<213> 通过大肠杆菌Genscript遗传密码子频率对DfHAD-6His-GST进行密码子优化的序列

<400> 37

Met Ser Gly Ser His His His His His His Ser Ser Gly Met Ser Pro

1 5 10 15

Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro Thr Arg Leu

20 25 30

Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu Tyr Glu Arg

35 40 45

Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu Gly Leu Glu

50 55 60

Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys Leu Thr Gln

65 70 75 80

Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn Met Leu Gly

85 90 95

Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu Gly Ala Val

100 105 110

Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser Lys Asp Phe

115 120 125

Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu Met Leu Lys

130 135 140

Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn Gly Asp His

145 150 155 160

Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp Val Val Leu

165 170 175

Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu Val Cys Phe

180 185 190

Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr Leu Lys Ser

195 200 205

Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala Thr Phe Gly

210 215 220

Gly Gly Asp His Pro Pro Lys Ser Asp Leu Gly His Thr Gly His Arg

225 230 235 240

Ser Asp Asp Asp Asp Lys His Met Glu Phe Ser Ala Ser Ala Pro Pro

245 250 255

Pro Arg Leu Ala Ser Val Ile Ile Leu Glu Pro Leu Gly Phe Leu Leu

260 265 270

Thr Pro His Tyr Ser Ser Gln Leu Pro Lys Lys Leu Leu Arg Arg Leu

275 280 285

Leu Cys Thr Arg Ile Trp His Arg Tyr Gln Arg Gly Arg Leu Arg Leu

290 295 300

Arg Asp Ala Ala Met Leu Leu Ala Gln Leu Pro Phe Leu Ala Val Ser

305 310 315 320

Asp His Pro Trp Ala Leu Asp Asn Leu Ala Ser Leu Leu Arg Pro Thr

325 330 335

Ala Val Arg Ala Val Pro Trp Met Leu Leu Leu Leu Asp Phe Leu Arg

340 345 350

Asp Glu Leu His Leu Lys Val Val Cys Ala Thr Asn Ser Ser Pro Glu

355 360 365

Glu Leu Gln Glu Leu Arg His Gln Phe Pro Ala Leu Phe Ala Lys Val

370 375 380

Asp Ala Thr Val Ser Ser Gly Glu Glu Gly Val Gly Lys Pro Ser Val

385 390 395 400

Arg Phe Leu Gln Ala Ala Leu Asp Lys Ala Gly Val His Ala Gln Gln

405 410 415

Thr Leu Tyr Leu Asp Ser Phe Asp Ser Leu Glu Thr Ile Met Ala Ala

420 425 430

Arg Ser Leu Gly Met His Ala Leu Ser Val Glu Pro Cys His Ile Asp

435 440 445

Glu Leu Thr Ala Arg Ala Ser Ser Gly Gln Leu Arg Asp Ala Gln Leu

450 455 460

Ile Arg Arg Ile Val Cys Ala Met His Gly Pro Ala Val Ser Ala Val

465 470 475 480

Val Ser Gly Ser Ile Thr Ser Ser Gly Pro Gln Thr Ala Lys Ile Glu

485 490 495

Glu Leu Pro Thr Ala Ala Asp Ser His Leu Arg Ser Ala Ala Leu Thr

500 505 510

Ser Ala Gln Gln Phe Phe Leu Lys Val Ile Ala Pro His Arg Pro Glu

515 520 525

Lys Pro Phe Val Gln Leu Pro Ser Leu Thr Ser Glu Gly Ile Arg Ile

530 535 540

Tyr Asp Thr Phe Ala Gln Phe Val Ile Ala Asp Leu Leu Asp Asp Thr

545 550 555 560

Arg Phe Leu Pro Met Gln Ser Pro Pro Pro Asn Gly Leu Ile Thr Phe

565 570 575

Val Asn Pro Ser Ala Tyr Leu Ala Asp Asp Ile Lys Asn Gly Asn Ser

580 585 590

His Ile Val Pro Gly Val Gln Phe Tyr Ala Ser Asp Ala Cys Thr Leu

595 600 605

Ile Asp Ile Pro His Asp Leu Asp Thr Thr Ser Val Gly Leu Ser Val

610 615 620

Leu His Lys Phe Gly Lys Val Asp Lys Asp Thr Leu Asn Lys Val Leu

625 630 635 640

Asp Arg Met Leu Glu Gln Val Ser Glu Asp Asp Gly Ile Leu Gln Val

645 650 655

Tyr Phe Asp Val Glu Arg Pro Arg Ile Asp Pro Val Val Val Ala Asn

660 665 670

Thr Val Phe Leu Phe His Leu Gly Lys Arg Gly His Glu Val Ala Arg

675 680 685

Ser Glu Lys Phe Val Glu Ser Val Leu Leu Gln Arg Ala Tyr Glu Glu

690 695 700

Gly Thr Leu Tyr Tyr Asn Leu Gly Glu Ala Phe Leu Val Ser Val Ala

705 710 715 720

Arg Leu Val His Glu Phe Lys Glu His Phe Thr Arg Ser Gly Met Arg

725 730 735

Arg Ala Leu Glu Glu Arg Leu Arg Glu Arg Ala Arg Ala Gly Met Gln

740 745 750

Glu Arg Asp Asp Ala Leu Ala Leu Ala Met Arg Ile Arg Ala Cys Ala

755 760 765

Leu Cys Gly Leu Ala Gly Glu Gly Leu Thr Lys Ala Ala Glu Gln Glu

770 775 780

Leu Leu Arg Leu Gln Cys Lys Ser Lys Gly Cys Trp Gly Cys His Pro

785 790 795 800

Phe Tyr Arg Asn Gly Ser Asn Val Leu Ser Trp Ile Gly Ser Glu Ala

805 810 815

Leu Thr Thr Ala Tyr Ala Ile Ala Ala Leu Gln Pro Ile Asp Ile

820 825 830

<210> 38

<211> 526

<212> PRT

<213> 隐孔菌(Cryptoporus volvatus)_CvTps1蛋白

<400> 38

Met Thr Thr Ile His Arg Arg His Thr Thr Leu Ile Leu Asp Leu Gly

1 5 10 15

Asp Val Leu Phe Arg Trp Ser Pro Lys Thr Glu Thr Ala Ile Pro Pro

20 25 30

Arg Gln Leu Lys Glu Ile Leu Thr Ser Val Thr Trp Phe Glu Tyr Glu

35 40 45

Arg Gly Gln Ile Ser Gln Thr Glu Cys Tyr Glu Arg Cys Ala Ala Glu

50 55 60

Phe Lys Val Asp Pro Leu Val Ile Ala Glu Ala Phe Lys Gln Ala Arg

65 70 75 80

Glu Ser Leu Arg Pro Asn Lys Ala Phe Ile Ala Leu Ile Arg Glu Leu

85 90 95

Arg His Gln Met His Gly Asp Leu Thr Val Leu Ala Leu Ser Asn Ile

100 105 110

Ser Leu Pro Asp Tyr Glu Tyr Ile Met Ser Leu Ser Ser Asp Trp Ala

115 120 125

Thr Val Phe Asn Arg Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys

130 135 140

Pro His Leu Gly Cys Tyr Arg Lys Val Ile Ser Glu Met Ser Leu Glu

145 150 155 160

Pro Gln Thr Thr Val Phe Val Asp Asp Lys Leu Asp Asn Val Ala Ser

165 170 175

Ala Arg Ser Leu Gly Met His Gly Ile Val Phe Asp Asn Glu Ala Asn

180 185 190

Val Phe Arg Gln Leu Arg Asn Ile Phe Gly Asn Pro Val Ser Arg Gly

195 200 205

Gln Gly Tyr Leu Arg Lys His Ala Gly Lys Leu Glu Ser Ser Thr Asp

210 215 220

Asn Gly Leu Thr Phe Glu Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu

225 230 235 240

Val Thr Gln Asp Arg Ser Leu Ile Thr Leu Ser Glu Cys Pro Arg Thr

245 250 255

Trp Asn Phe Phe Arg Gly Gln Pro Leu Phe Ser Glu Ser Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Val Ala Leu Thr Val Leu Gln Pro Asp Arg

275 280 285

Ala Leu Val Asp Ser Ile Leu Asp Gln Met Leu Glu Tyr Val Asp Ala

290 295 300

Asp Gly Ile Met Gln Thr Tyr Phe Asp Ser Ser Arg Pro Arg Ile Asp

305 310 315 320

Pro Phe Val Cys Val Asn Val Leu Ser Leu Phe Tyr Ala Asn Gly Arg

325 330 335

Gly Arg Glu Leu Pro His Thr Leu Glu Trp Val Tyr Glu Val Leu Leu

340 345 350

His Arg Ala Tyr His Gly Gly Ser Arg Tyr Tyr Leu Ser Pro Asp Cys

355 360 365

Phe Leu Phe Phe Met Ser Arg Leu Leu Lys Arg Ala Asn Asp Ser Ala

370 375 380

Leu Gln Ala Arg Phe Arg Pro Leu Phe Met Glu Arg Val Lys Glu Arg

385 390 395 400

Val Gly Ala Ala Gly Asp Ser Met Asp Leu Ala Phe Arg Ile Leu Ala

405 410 415

Ala Ala Thr Ile Gly Val His Cys Pro Gln Asp Leu Glu Arg Leu Ala

420 425 430

Ala Ala Gln Cys Glu Asp Gly Gly Trp Asp Met Cys Trp Phe Tyr Ala

435 440 445

Phe Gly Ser Thr Gly Ile Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala

450 455 460

Leu Ala Val Ala Ala Ile Arg Thr Ala Leu Gly Arg Pro Pro Ser Pro

465 470 475 480

Ser Pro Ser Asn Ile Ser Ser Ser Ser Lys Leu Asp Ala Pro Asn Ser

485 490 495

Phe Leu Gly Ile Pro Arg Pro Thr Ser Pro Ile Arg Phe Gly Glu Leu

500 505 510

Phe Arg Ser Trp Arg Lys Asn Lys Pro Thr Ala Lys Ser Gln

515 520 525

<210> 39

<211> 1956

<212> DNA

<213> 隐孔菌(Cryptoporus volvatus)_CvTps1转录物（包括非编码序列）

<400> 39

catcccgcct tttgagcatg gcacacaaac agcctttaag gagctccttg gttgcctagt 60

catgcctcca cctgccccct cctcactcat cccctcgcat cctaaaacat gaccacgatt 120

caccgtcggc acaccactct catcttggac ctcggcgacg tcctcttccg ctggtcacca 180

aagaccgaga ccgccatccc ccctcggcag cttaaggaga tacttacctc cgtcacctgg 240

ttcgagtacg aacgaggcca gatatcccaa acagaatgtt acgaacgatg cgctgcagaa 300

ttcaaagtcg accccttagt gatcgctgaa gccttcaagc aagctcgcga gtcattacgg 360

cccaacaaag cgttcatcgc cttgattcgc gaacttcgcc atcaaatgca tggagacctc 420

acggtcctcg ccctttccaa catttccctc cccgattacg aatatatcat gtctctgagc 480

tcggattggg caaccgtctt caatcgcgta ttcccttctg cacttgttgg cgagcgaaaa 540

ccccatctgg ggtgctaccg caaggtcatt tcggagatga gcttggaacc ccagacaacc 600

gtatttgtcg atgataagct agacaacgtc gcctctgctc gctcacttgg catgcacggc 660

atcgtattcg acaacgaagc caatgtcttc cggcaactgc gcaatatctt cgggaatccg 720

gttagccgcg gtcaaggcta tcttcgcaag catgccggaa agcttgagtc ttctaccgac 780

aatggcttga cctttgagga gaacttcacc cagctcatca tctacgaggt gacacaagac 840

aggagtctca tcacgctctc agaatgtccc cgtacctgga atttctttcg aggtcaaccg 900

ctcttctcgg agtctttccc ggatgatgtg gacacaacat ccgtggcatt gacagtacta 960

caacccgata gagcgctcgt tgattctatt ctagaccaaa tgcttgaata tgttgacgcc 1020

gacggcatca tgcagacata cttcgacagc tcgcgaccac gcatagaccc ttttgtttgc 1080

gtcaatgtgc tttctctgtt ctacgcaaac ggccggggtc gggagctccc tcacacactg 1140

gagtgggtct atgaagtact cctgcatcgc gcctaccatg gaggctcacg ttactaccta 1200

tcaccggact gctttttatt cttcatgagc cgcttgctca agcgcgccaa cgactcggcc 1260

ctccaggctc ggttccgccc actgttcatg gagagagtga aagaacgagt aggggcagcc 1320

ggagactcaa tggacctggc cttccgcatc ctcgccgcgg ctaccattgg cgtccattgc 1380

ccccaagatc tagaaagatt ggccgccgcg caatgcgagg acggtggatg ggacatgtgc 1440

tggttctacg cgttcgggtc gacaggtatc aaggcgggca accgcggcct caccacggcc 1500

cttgccgtcg cagctatacg aaccgccctc gggcgccccc cctctcccag cccctccaac 1560

atctcgtcgt cgtcgaagct cgacgctccc aacagcttct tgggcatccc gcgcccaacc 1620

agccccattc gctttggcga acttttccgt tcctggcgaa agaacaaacc gaccgcaaaa 1680

tctcaatgaa tctcaggttc tcgtgctctc gtgctatctt cgtacttatg ctactcgaca 1740

ttacccgtcg ctgtctacaa tgatacgggt actttgatga aactgtagat gtatttgtat 1800

catattgacc tccatccata gtcacctagc tactgttcgt gttatcacct gttgctgtta 1860

tatgatacaa gatgcccaaa cgagaatgta gaaatgttcc gtacacttgt gtacctgtga 1920

tgaagctaca taggccttca atcgatcact tggtcc 1956

<210> 40

<211> 1581

<212> DNA

<213> 隐孔菌(Cryptoporus volvatus)_CvTps1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 40

atgaccacga ttcaccgtcg gcacaccact ctcatcttgg acctcggcga cgtcctcttc 60

cgctggtcac caaagaccga gaccgccatc ccccctcggc agcttaagga gatacttacc 120

tccgtcacct ggttcgagta cgaacgaggc cagatatccc aaacagaatg ttacgaacga 180

tgcgctgcag aattcaaagt cgacccctta gtgatcgctg aagccttcaa gcaagctcgc 240

gagtcattac ggcccaacaa agcgttcatc gccttgattc gcgaacttcg ccatcaaatg 300

catggagacc tcacggtcct cgccctttcc aacatttccc tccccgatta cgaatatatc 360

atgtctctga gctcggattg ggcaaccgtc ttcaatcgcg tattcccttc tgcacttgtt 420

ggcgagcgaa aaccccatct ggggtgctac cgcaaggtca tttcggagat gagcttggaa 480

ccccagacaa ccgtatttgt cgatgataag ctagacaacg tcgcctctgc tcgctcactt 540

ggcatgcacg gcatcgtatt cgacaacgaa gccaatgtct tccggcaact gcgcaatatc 600

ttcgggaatc cggttagccg cggtcaaggc tatcttcgca agcatgccgg aaagcttgag 660

tcttctaccg acaatggctt gacctttgag gagaacttca cccagctcat catctacgag 720

gtgacacaag acaggagtct catcacgctc tcagaatgtc cccgtacctg gaatttcttt 780

cgaggtcaac cgctcttctc ggagtctttc ccggatgatg tggacacaac atccgtggca 840

ttgacagtac tacaacccga tagagcgctc gttgattcta ttctagacca aatgcttgaa 900

tatgttgacg ccgacggcat catgcagaca tacttcgaca gctcgcgacc acgcatagac 960

ccttttgttt gcgtcaatgt gctttctctg ttctacgcaa acggccgggg tcgggagctc 1020

cctcacacac tggagtgggt ctatgaagta ctcctgcatc gcgcctacca tggaggctca 1080

cgttactacc tatcaccgga ctgcttttta ttcttcatga gccgcttgct caagcgcgcc 1140

aacgactcgg ccctccaggc tcggttccgc ccactgttca tggagagagt gaaagaacga 1200

gtaggggcag ccggagactc aatggacctg gccttccgca tcctcgccgc ggctaccatt 1260

ggcgtccatt gcccccaaga tctagaaaga ttggccgccg cgcaatgcga ggacggtgga 1320

tgggacatgt gctggttcta cgcgttcggg tcgacaggta tcaaggcggg caaccgcggc 1380

ctcaccacgg cccttgccgt cgcagctata cgaaccgccc tcgggcgccc cccctctccc 1440

agcccctcca acatctcgtc gtcgtcgaag ctcgacgctc ccaacagctt cttgggcatc 1500

ccgcgcccaa ccagccccat tcgctttggc gaacttttcc gttcctggcg aaagaacaaa 1560

ccgaccgcaa aatctcaatg a 1581

<210> 41

<211> 1581

<212> DNA

<213> 人工序列_CvTps1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 41

atgactacga tccaccgccg ccatactacg ctgatcctgg acctgggtga tgttctgttc 60

cgctggtccc cgaaaaccga aaccgcaatt ccgcctcgtc agctgaaaga aatcttgacc 120

agcgttacct ggttcgagta tgagcgtggc caaattagcc agaccgaatg ctacgagcgt 180

tgtgctgccg agtttaaagt tgatccgctg gttattgccg aagcgtttaa acaagcgcgt 240

gaaagcctgc gtccgaacaa agcgtttatc gcgttgatcc gtgagttgcg ccaccagatg 300

catggtgacc tgacggtcct ggcactgagc aacattagcc tgcctgatta tgagtacatt 360

atgtcgctga gctccgattg ggcgacggtc tttaatcgcg tgtttccgag cgcactggtg 420

ggtgagcgta agccacacct gggttgctac cgcaaggtca tcagcgagat gtctctggag 480

ccgcagacca cggttttcgt cgatgacaaa ctggacaatg tcgcaagcgc tcgtagcctg 540

ggcatgcatg gcatcgtgtt cgacaacgaa gcgaacgttt ttcgtcagct gcgtaatatc 600

ttcggtaacc cggttagccg cggtcaaggt tacttgcgta aacacgccgg taaactggaa 660

tctagcacgg ataatggtct gaccttcgaa gagaacttca ctcaattaat tatttacgaa 720

gtcacgcaag accgcagcct gatcaccctg agcgagtgcc cgcgtacctg gaacttcttc 780

cgcggtcaac cactgttttc tgagagcttt ccggacgacg tggacaccac ctctgtggcg 840

ttgaccgttc tgcagccgga tcgtgcgttg gtggatagca tcctggacca gatgttggaa 900

tatgttgacg cggatggtat tatgcaaacc tactttgatt catcccgtcc gcgcattgac 960

ccgttcgtgt gcgtgaatgt cctgagcctg ttctacgcca atggcagagg ccgcgagctg 1020

ccacacacgc tggaatgggt ctatgaagtt ctgctgcacc gtgcgtacca cggcggtagc 1080

cgttattacc tgagcccgga ctgtttcctg ttctttatga gccgtctgct gaagcgcgcg 1140

aatgactcgg cgctgcaggc ccgttttcgc ccgcttttca tggaacgtgt gaaagagcgt 1200

gtgggcgcag ccggcgatag catggacctg gcgttccgca ttctggccgc tgcaaccatc 1260

ggcgttcatt gcccacaaga tctggagcgt ctggcagcag cgcagtgcga agatggtggc 1320

tgggatatgt gttggtttta tgcgtttggc agcacgggta tcaaggctgg caaccgcggt 1380

ctgaccaccg cgttggctgt cgccgcaatt cgtaccgcgc tgggtcgtcc gccttccccg 1440

agcccgagca atatttctag ctccagcaaa ctggacgcgc cgaactcctt cctgggcatc 1500

ccgcgtccga ccagcccgat ccgtttcggt gaactgtttc gtagctggcg taagaacaag 1560

ccgaccgcga aaagccagta a 1581

<210> 42

<211> 521

<212> PRT

<213> 药用拟层孔菌(Laricifomes officinalis)_LoTps1蛋白

<400> 42

Met Tyr Thr Ala Leu Ile Leu Asp Leu Gly Asp Val Leu Phe Ser Trp

1 5 10 15

Ser Ser Thr Thr Asn Thr Thr Ile Pro Pro Arg Gln Leu Lys Glu Ile

20 25 30

Leu Ser Ser Pro Ala Trp Phe Glu Tyr Glu Arg Gly Arg Ile Thr Gln

35 40 45

Ala Glu Cys Tyr Glu Arg Val Ser Ala Glu Phe Ser Leu Asp Ala Thr

50 55 60

Ala Val Ala Glu Ala Phe Arg Gln Ala Arg Asp Ser Leu Arg Pro Asn

65 70 75 80

Asp Lys Phe Leu Thr Leu Ile Arg Glu Leu Arg Gln Gln Ser His Gly

85 90 95

Glu Leu Thr Val Leu Ala Leu Ser Asn Ile Ser Leu Pro Asp Tyr Glu

100 105 110

Phe Ile Met Ala Leu Asp Ser Lys Trp Thr Ser Val Phe Asp Arg Val

115 120 125

Phe Pro Ser Ala Leu Val Gly Glu Arg Lys Pro His Leu Gly Ala Phe

130 135 140

Arg Gln Val Leu Ser Glu Met Asn Leu Asp Pro His Thr Thr Val Phe

145 150 155 160

Val Asp Asp Lys Leu Asp Asn Val Val Ser Ala Arg Ser Leu Gly Met

165 170 175

His Gly Val Val Phe Asp Ser Gln Asp Asn Val Phe Arg Met Leu Arg

180 185 190

Asn Ile Phe Gly Asp Pro Ile His Arg Gly Arg Asp Tyr Leu Arg Gln

195 200 205

His Ala Gly Arg Leu Glu Thr Ser Thr Asp Ala Gly Val Val Phe Glu

210 215 220

Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu Leu Thr Asn Asp Lys Ser

225 230 235 240

Leu Ile Thr Thr Ser Asn Cys Ala Arg Thr Trp Asn Phe Phe Arg Gly

245 250 255

Lys Pro Leu Phe Ser Ala Ser Phe Pro Asp Asp Met Asp Thr Thr Ser

260 265 270

Val Ala Leu Thr Val Leu Arg Leu Asp His Ala Leu Val Asn Ser Val

275 280 285

Leu Asp Glu Met Leu Lys Tyr Val Asp Ala Asp Gly Ile Met Gln Thr

290 295 300

Tyr Phe Asp His Thr Arg Pro Arg Met Asp Pro Phe Val Cys Val Asn

305 310 315 320

Val Leu Ser Leu Phe His Glu Gln Gly Arg Gly His Glu Leu Pro Asn

325 330 335

Thr Leu Glu Trp Val His Glu Val Leu Leu His Arg Ala Tyr Ile Gly

340 345 350

Gly Ser Arg Tyr Tyr Leu Ser Ala Asp Cys Phe Leu Phe Phe Met Ser

355 360 365

Arg Leu Leu Gln Arg Ile Thr Asp Pro Ser Val Leu Gly Arg Phe Arg

370 375 380

Pro Leu Phe Ile Glu Arg Val Arg Glu Arg Val Gly Ala Thr Gly Asp

385 390 395 400

Ser Ile Asp Leu Ala Phe Arg Ile Ile Ala Ala Ser Thr Val Gly Ile

405 410 415

Gln Cys Pro Arg Asp Leu Glu Ser Leu Leu Ala Ala Gln Cys Glu Asp

420 425 430

Gly Gly Trp Asp Leu Cys Trp Phe Tyr Gln Tyr Gly Ser Thr Gly Val

435 440 445

Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala Leu Ala Ile Lys Ala Ile

450 455 460

Asp Ser Ala Ile Ala Arg Pro Pro Ser Pro Ala Leu Ser Val Ala Ser

465 470 475 480

Ser Ser Lys Ser Glu Ile Pro Lys Pro Ile Gln Arg Ser Leu Arg Pro

485 490 495

Leu Ser Pro Arg Arg Phe Gly Gly Phe Leu Met Pro Trp Arg Arg Ser

500 505 510

Gln Arg Asn Gly Val Ala Val Ser Ser

515 520

<210> 43

<211> 2291

<212> DNA

<213> 药用拟层孔菌(Laricifomes officinalis)_LoTps1转录物（包括非编码序列）

<400> 43

gcgtctgctg cggtctctca ccgcgccgag cgacgggaag cggaggcttt ttgatgcagc 60

cagctcagcg ccatcctctc acgcaggggg tttgatccag atctgatcgc ctccgggttc 120

tcatctagaa cgcacggcgg ctcccaggaa gttctatcga ccctctgcgc gctggtcggc 180

ggcacgatgt ggctacacca gtcccaatca tatctcacac ccagcaccat catctcgggc 240

ctcttcgtca tgtaaccctc ccaagcctat ttttcagggc gttccccctc accggcgcgc 300

ttcttaaaga atcccgaaat gtatacggct cttatccttg acctcggcga cgttctgttc 360

tcttggtcgt cgacgaccaa cacgactatt ccccctcggc agctaaagga gatcctctca 420

tctcctgcct ggtttgagta cgagcgtggt cgcataacgc aagccgaatg ctacgagcgt 480

gtcagcgccg agttcagcct agacgccacc gccgtcgcgg aagcattccg gcaagctcgc 540

gactccttgc gcccgaacga caagttcctc acgttaattc gcgagcttcg acaacaatct 600

catggggagc tcacggtgct tgcgctgtcc aacatatccc ttcccgacta tgaattcatc 660

atggccctcg actcgaagtg gacttctgtc tttgaccgcg tcttcccttc tgccctcgtg 720

ggcgaacgga agccacacct tggagcgttt cgccaggttc tgtccgagat gaatcttgac 780

ccgcacacaa ctgtgttcgt cgatgacaag ctggacaatg tcgtctccgc acggtccctc 840

gggatgcacg gcgtcgtgtt cgactcccaa gacaatgtct ttcggatgct gagaaacatc 900

tttggcgatc ccattcatcg gggacgtgac tatctccgac agcacgccgg acgtctggag 960

acctccacgg atgccggtgt ggtcttcgaa gagaatttca cgcaactcat catctacgaa 1020

ctgacgaatg acaagtctct catcacgaca tcaaactgtg ctcgtacttg gaatttcttt 1080

cgtgggaagc ctttgttctc agcatcgttc cctgacgaca tggacacgac ctcggttgcc 1140

ttgactgtat tacgtttaga ccacgccctc gtgaactcgg ttttggacga gatgctaaag 1200

tatgtcgacg cagacggcat catgcagacc tacttcgacc atacacgccc acgcatggat 1260

ccatttgtct gcgtcaatgt gctctcgttg tttcacgaac aaggtcgtgg ccacgagctt 1320

ccgaacaccc tcgaatgggt ccatgaggtc ctcctccacc gcgcgtacat cgggggctcg 1380

cggtactacc tctccgcgga ctgcttcctc tttttcatga gccgcctcct gcagcgcatc 1440

accgacccgt ccgtccttgg ccgcttccgt ccactattca tagagcgcgt tcgggagcgt 1500

gtaggtgcga ccggggactc catcgatctc gcattccgca tcatcgccgc gtccacagta 1560

ggcatccagt gtccacgcga cttggaaagt ctcctcgccg cacagtgtga agacggtggc 1620

tgggacctgt gctggttcta ccagtacgga tcgaccggtg tcaaggcggg caaccgcggg 1680

ctcaccaccg ctctggcgat caaagctatt gactccgcca ttgcgaggcc accttcgcct 1740

gccctctcag tcgcttcgtc gtccaaatcg gagataccga aacccataca acggtccctt 1800

aggcccctta gcccccgccg gtttggcggt ttcctgatgc cgtggcgcag gtcacagcgc 1860

aatggcgtgg cggtctctag ttgaacactt gacccttgac acttcgcttt gcactgcctg 1920

ctcccctgcc aatcctcccc tacgatcgta tcatccctct cttgccctcg cctccccctc 1980

gtaccccctc tcatggggtg ccatttgtag atatgtacgt agcgtgatgt agcggtactc 2040

ggatcgttct cgtactcgtc ttgctctgcc gtcgcttcca gcccgtgctg ttctctcgtt 2100

caggctattc gttggttacg cgtatatcgt aatagaccgc cccggttcct cgcctacaga 2160

cactcgcccg tctcgccacg gactcggcta cggattcaga ctacatgagt ggcagttatc 2220

acacgcagat ccctccttgg tcgttctgta gtacccacat atgtaattgt accagtccac 2280

tgttgcagat c 2291

<210> 44

<211> 1566

<212> DNA

<213> 药用拟层孔菌(Laricifomes officinalis)_LoTps1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 44

atgtatacgg ctcttatcct tgacctcggc gacgttctgt tctcttggtc gtcgacgacc 60

aacacgacta ttccccctcg gcagctaaag gagatcctct catctcctgc ctggtttgag 120

tacgagcgtg gtcgcataac gcaagccgaa tgctacgagc gtgtcagcgc cgagttcagc 180

ctagacgcca ccgccgtcgc ggaagcattc cggcaagctc gcgactcctt gcgcccgaac 240

gacaagttcc tcacgttaat tcgcgagctt cgacaacaat ctcatgggga gctcacggtg 300

cttgcgctgt ccaacatatc ccttcccgac tatgaattca tcatggccct cgactcgaag 360

tggacttctg tctttgaccg cgtcttccct tctgccctcg tgggcgaacg gaagccacac 420

cttggagcgt ttcgccaggt tctgtccgag atgaatcttg acccgcacac aactgtgttc 480

gtcgatgaca agctggacaa tgtcgtctcc gcacggtccc tcgggatgca cggcgtcgtg 540

ttcgactccc aagacaatgt ctttcggatg ctgagaaaca tctttggcga tcccattcat 600

cggggacgtg actatctccg acagcacgcc ggacgtctgg agacctccac ggatgccggt 660

gtggtcttcg aagagaattt cacgcaactc atcatctacg aactgacgaa tgacaagtct 720

ctcatcacga catcaaactg tgctcgtact tggaatttct ttcgtgggaa gcctttgttc 780

tcagcatcgt tccctgacga catggacacg acctcggttg ccttgactgt attacgttta 840

gaccacgccc tcgtgaactc ggttttggac gagatgctaa agtatgtcga cgcagacggc 900

atcatgcaga cctacttcga ccatacacgc ccacgcatgg atccatttgt ctgcgtcaat 960

gtgctctcgt tgtttcacga acaaggtcgt ggccacgagc ttccgaacac cctcgaatgg 1020

gtccatgagg tcctcctcca ccgcgcgtac atcgggggct cgcggtacta cctctccgcg 1080

gactgcttcc tctttttcat gagccgcctc ctgcagcgca tcaccgaccc gtccgtcctt 1140

ggccgcttcc gtccactatt catagagcgc gttcgggagc gtgtaggtgc gaccggggac 1200

tccatcgatc tcgcattccg catcatcgcc gcgtccacag taggcatcca gtgtccacgc 1260

gacttggaaa gtctcctcgc cgcacagtgt gaagacggtg gctgggacct gtgctggttc 1320

taccagtacg gatcgaccgg tgtcaaggcg ggcaaccgcg ggctcaccac cgctctggcg 1380

atcaaagcta ttgactccgc cattgcgagg ccaccttcgc ctgccctctc agtcgcttcg 1440

tcgtccaaat cggagatacc gaaacccata caacggtccc ttaggcccct tagcccccgc 1500

cggtttggcg gtttcctgat gccgtggcgc aggtcacagc gcaatggcgt ggcggtctct 1560

agttga 1566

<210> 45

<211> 1566

<212> DNA

<213> 人工序列_LoTps1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 45

atgtacacgg cgctgatttt ggatttgggt gatgttctgt ttagctggag ctcaacgact 60

aacaccacca ttccgccgcg tcagctgaaa gaaatcttga gctccccggc gtggttcgag 120

tacgagcgtg gccgtatcac ccaggcagag tgttatgagc gtgtcagcgc agagtttagc 180

ctggatgcga cggccgtggc tgaggctttt cgtcaggcac gtgatagcct gcgtccgaac 240

gacaaatttc tgaccctgat ccgtgagctg cgtcaacaga gccacggtga attgaccgtt 300

ctggccttgt ctaacatcag cctgccggat tacgaattta ttatggcact ggactcgaag 360

tggaccagcg tgtttgatcg tgtgttcccg agcgccctgg tgggcgaacg caagccgcac 420

ctgggcgcgt tccgccaagt cctgtccgag atgaatttgg acccgcatac caccgttttt 480

gtggacgaca aactggacaa tgttgtcagc gcacgcagcc tgggtatgca cggtgtcgtg 540

ttcgacagcc aagacaatgt ttttcgtatg ctgcgtaaca ttttcggtga cccaattcac 600

cgcggtcgtg actatctgcg ccagcacgct ggtcgtcttg aaacgtccac cgatgcgggc 660

gttgtgttcg aagagaactt cacccaactg atcatttacg aactgaccaa cgataagagc 720

ctgatcacca cctctaattg cgcccgcacc tggaacttct tccgcggcaa acctctgttc 780

tccgcgagct ttccggacga tatggacact acgtcggtag cgctgaccgt gctgcgtctg 840

gaccatgcgc tggtgaatag cgttctggat gaaatgctga aatacgtcga tgctgacggt 900

attatgcaga cctactttga tcatacgcgt cctcgtatgg acccgttcgt ttgcgtcaat 960

gtgctgagcc tgtttcacga gcaaggtcgc ggtcatgaac tgccgaatac gctggaatgg 1020

gtgcatgaag tcctgctgca ccgtgcgtat atcggtggca gccgctatta tctgagcgcg 1080

gattgtttcc tgttctttat gagccgtctg ttgcaacgta ttaccgaccc gagcgtttta 1140

ggtagatttc gcccgctgtt catcgagcgt gttcgcgagc gcgttggcgc gactggcgac 1200

agcatcgacc tggcattccg tatcatcgcg gccagcacgg tcggcattca atgcccgcgt 1260

gacctggagt ctctgctggc agcacagtgc gaagatggtg gctgggatct gtgttggttt 1320

taccagtacg gcagcacggg tgttaaggcc ggtaaccgtg gtctgaccac ggcgttggcg 1380

atcaaagcga ttgacagcgc catcgcgcgt ccgccaagcc cggccctgtc cgttgcaagc 1440

tccagcaaga gcgagattcc gaagccgatt cagcgtagcc tccgcccgtt gagcccgcgt 1500

cgcttcggtg gcttcctgat gccgtggcgt cgtagccaac gcaatggtgt cgcggtgagc 1560

tcttaa 1566

<210> 46

<211> 527

<212> PRT

<213> 白腐菌(Obba rivulosa)_OCH93767.1蛋白

<400> 46

Met Ser Ala Ala Val Arg Tyr Thr Thr Leu Ile Leu Asp Leu Gly Asp

1 5 10 15

Val Leu Phe Thr Trp Ser Pro Lys Thr Lys Thr Ser Ile Ser Pro Arg

20 25 30

Ile Leu Lys Glu Ile Leu Asn Ser Ala Thr Trp Tyr Glu Tyr Glu Arg

35 40 45

Gly Ser Ile Thr Gln His Glu Cys Tyr Glu Arg Val Gly Val Glu Phe

50 55 60

Gly Ile Ala Pro Ser Glu Ile His Asn Ala Phe Lys Gln Ala Arg Asp

65 70 75 80

Ser Met Glu Ser Asn Asp Glu Leu Ile Ala Leu Val Arg Glu Leu Lys

85 90 95

Glu Gln Ser Asp Gly Glu Leu Leu Val Phe Ala Leu Ser Asn Ile Ser

100 105 110

Leu Pro Asp Tyr Glu Tyr Val Leu Thr Lys Pro Ala Asp Trp Ser Ile

115 120 125

Phe Asp Lys Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys Pro His

130 135 140

Leu Gly Ile Tyr Lys His Val Ile Ala Glu Thr Gly Val Asp Pro Arg

145 150 155 160

Thr Thr Val Phe Val Asp Asp Lys Ile Asp Asn Val Leu Ser Ala Arg

165 170 175

Ser Leu Gly Met His Gly Ile Val Phe Asp Lys His Glu Asp Val Met

180 185 190

Arg Ala Leu Arg Asn Ile Phe Gly Asp Pro Val Arg Arg Gly Arg Glu

195 200 205

Tyr Leu Arg Arg Asn Ala Arg Lys Leu Glu Ser Ile Thr Asp His Gly

210 215 220

Val Ala Phe Gly Glu Asn Phe Thr Gln Leu Leu Ile Leu Glu Leu Thr

225 230 235 240

Ser Asp Ala Ser Leu Val Thr Leu Pro Asp Arg Pro Arg Thr Trp Asn

245 250 255

Phe Phe Arg Gly Lys Pro Leu Phe Ser Glu Ala Phe Pro Asp Asp Leu

260 265 270

Asp Thr Thr Ser Leu Ala Leu Thr Val Leu Lys Arg Asp Ala Ala Thr

275 280 285

Val Ser Ser Val Met Asp Glu Met Leu Lys Tyr Arg Asp Ala Asp Gly

290 295 300

Ile Met Gln Thr Tyr Phe Asp Asn Gly Arg Gln Arg Leu Asp Pro Phe

305 310 315 320

Val Asn Ala Asn Val Leu Thr Leu Phe Tyr Ala Asn Gly Arg Gly His

325 330 335

Glu Leu Asp Gln Ser Leu Ser Trp Val Arg Glu Val Leu Leu Tyr Arg

340 345 350

Ala Tyr Leu Gly Gly Ser Arg Tyr Tyr Pro Ser Ala Asp Cys Phe Leu

355 360 365

Tyr Phe Ile Ser Arg Leu Phe Ala Cys Thr Ser Asp Pro Val Leu His

370 375 380

His Gln Leu Lys Pro Leu Phe Val Glu Arg Val His Glu Arg Ile Gly

385 390 395 400

Val Gln Gly Asp Ala Leu Glu Leu Ala Phe Arg Leu Leu Val Cys Ala

405 410 415

Ser Phe Asn Ile Ser Asn Gln Pro Asp Met Arg Lys Leu Leu Glu Met

420 425 430

Gln Cys Gln Asp Gly Gly Trp Asp Gly Gly Asn Leu Tyr Arg Phe Gly

435 440 445

Thr Thr Gly Leu Lys Val Thr Asn Arg Gly Leu Thr Thr Ala Ala Ala

450 455 460

Val Gln Ala Ile Glu Ala Thr Gln Leu Arg Pro Pro Ser Pro Ala Phe

465 470 475 480

Ser Val Glu Ser Pro Lys Ser Pro Val Thr Pro Val Thr Pro Met Leu

485 490 495

Glu Ile Pro Ala Leu Gly Leu Ser Ile Ser Arg Pro Ser Ser Pro Leu

500 505 510

Leu Gly Tyr Phe Lys Leu Pro Trp Lys Lys Ser Ala Glu Val His

515 520 525

<210> 47

<211> 1584

<212> DNA

<213> 白腐菌(Obba rivulosa)_OCH93767.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 47

atgtccgcag cagttcggta cacgaccctc atcctcgacc ttggcgacgt cttgttcact 60

tggtcaccga agacgaagac cagcatctcg cctcgtattc tgaaggagat cctgaattcc 120

gcgacctggt atgagtacga gcgcggtagt atcactcagc acgaatgtta cgaacgcgtt 180

ggcgtggagt tcggtattgc gccgagcgag atccacaacg cgttcaagca ggctcgggac 240

tctatggagt cgaatgacga gctgatcgcc cttgttcggg aactgaagga gcagtcagat 300

ggagagcttc tcgtcttcgc attatcgaac atctcactgc cggactacga atacgtcctg 360

acgaagcccg cggactggtc catcttcgac aaagtctttc cttccgctct cgtcggcgag 420

cgcaagcccc atctcggcat ctacaaacac gtcatcgcag agacgggcgt tgatccgcga 480

acaaccgtct tcgtggacga caagatcgac aatgtgcttt cggcgcggtc gctcggtatg 540

cacggcattg tcttcgacaa acacgaagac gtaatgcgcg ctctgcgaaa cattttcggt 600

gaccccgtgc gaagaggacg agaatatttg cgtcgaaatg caaggaaatt ggaatccatc 660

acagatcacg gcgtcgcctt cggggagaac ttcacccagc ttctgatcct cgaacttact 720

agtgatgcgt ccctcgttac tctccctgat cgtcctcgga catggaattt tttccgaggg 780

aagccgctct tttcggaggc cttccccgat gaccttgata ctacttcctt ggcactcact 840

gtcctgaaaa gagatgccgc cactgtatcg tccgtgatgg acgagatgct gaaatacagg 900

gacgcggacg gcatcatgca gacatacttc gacaacggtc ggcaacgact cgatccgttc 960

gtcaacgcca acgttttgac cctcttctac gccaacggtc gcggacacga gctggatcag 1020

agcctcagct gggttcgcga agtcttgctc taccgcgctt acctcggcgg ttcccgctac 1080

tacccctccg ccgactgctt cctatatttc atcagccgcc tcttcgcctg caccagcgac 1140

ccggtcctcc atcatcaact taagcccctc tttgttgagc gtgtgcacga gcggatagga 1200

gtgcagggcg acgcgctgga gctcgccttc cgcctgcttg tatgcgcgag cttcaacatc 1260

tcgaaccagc ctgacatgcg caagctgctc gagatgcagt gccaggacgg aggctgggat 1320

ggcggaaacc tgtatcgttt cggcaccacg ggcctcaagg tcacgaaccg gggtctgacc 1380

accgcagcag ccgtgcaagc catcgaggcg acgcagctgc gtccaccatc accggcgttc 1440

tctgtcgagt cgcctaagag cccggtgacg ccggtgacgc ccatgctgga gattccagcg 1500

ctgggtctca gcatctcgcg gccctccagt cctctgttgg ggtatttcaa gctcccgtgg 1560

aagaagtcag ccgaggttca ttga 1584

<210> 48

<211> 1584

<212> DNA

<213> 人工序列_OCH93767 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 48

atgtctgcag ctgttcgtta tactactctg atcctggatt tgggcgatgt tctgttcacc 60

tggtccccga aaaccaagac ctctatcagc ccacgtatcc tgaaagaaat cctgaacagc 120

gcgacctggt acgagtatga gcgtggcagc atcacccagc acgagtgcta cgagcgtgtt 180

ggcgtcgaat ttggtattgc gccgagcgag attcacaacg cgttcaaaca agcccgcgac 240

agcatggaat ccaacgacga actgattgct ctggtgcgtg agctgaaaga acagagcgat 300

ggtgagctgc tggtctttgc cctgagcaat atctctctgc cggattacga atacgttctg 360

accaaaccag cggactggtc aatcttcgat aaagtctttc cgagcgcttt ggtcggtgag 420

cgtaaaccgc atctgggtat ttacaaacac gttattgcgg aaaccggtgt tgacccgaga 480

acgaccgttt ttgttgacga taagattgac aacgtcctga gcgcacgcag cctgggtatg 540

catggtattg tctttgataa acacgaagat gtgatgcgtg ctctgcgcaa tatctttggc 600

gacccggtgc gtcgcggtcg tgagtatttg cgccgcaacg cgcgcaaatt ggagtccatt 660

accgatcatg gtgtcgcatt tggtgagaat ttcacccagc tcctgattct ggaactgacc 720

agcgacgcgt ccctggtgac gctgccggat cgtccgcgta cgtggaactt cttccgcggc 780

aagccgctgt ttagcgaagc gttcccggat gacctggaca ccacgagcct ggcactgacg 840

gtgctgaaac gcgatgcagc aactgtgagc tccgtcatgg acgaaatgct gaagtaccgc 900

gacgcggatg gcatcatgca gacgtatttc gacaacggtc gtcagcgtct ggacccgttt 960

gtcaacgcca atgttctgac gctgttttac gcgaatggcc gtggtcatga actggaccag 1020

agcttatcat gggtgcgtga agtgctgctg tatcgcgcct atctgggtgg cagccgctac 1080

tatccgagcg cggactgttt tctgtacttc attagccgct tgttcgcctg caccagcgat 1140

ccggttctgc atcaccaact gaagccattg ttcgtcgagc gtgtgcacga gcgtattggt 1200

gttcagggcg acgcactgga actggcgttc cgtctgttgg tgtgtgcgag cttcaacatt 1260

agcaatcagc cggatatgcg taagctgctg gaaatgcaat gccaagatgg cggctgggac 1320

ggtggtaatc tgtaccgttt tggcaccacc ggtttaaaag tgacgaatcg tggtttgacc 1380

accgctgcgg ccgttcaagc aattgaagca acgcaactgc gtccgccgag cccagcattt 1440

agcgtagagt cgcctaagag cccggttacg ccggtgacgc cgatgctgga aatcccggcg 1500

ctgggtctgt ctatcagccg tccgtcgagc ccgctgctgg gctatttcaa gttgccgtgg 1560

aagaaaagcg ccgaagtgca ctaa 1584

<210> 49

<211> 533

<212> PRT

<213> 弯孢胶化孔菌(Gelatoporia subvermispora)_EMD37666.1蛋白

<400> 49

Met Ser Ala Ala Ala Gln Tyr Thr Thr Leu Ile Leu Asp Leu Gly Asp

1 5 10 15

Val Leu Phe Thr Trp Ser Pro Lys Thr Lys Thr Ser Ile Pro Pro Arg

20 25 30

Thr Leu Lys Glu Ile Leu Asn Ser Ala Thr Trp Tyr Glu Tyr Glu Arg

35 40 45

Gly Arg Ile Ser Gln Asp Glu Cys Tyr Glu Arg Val Gly Thr Glu Phe

50 55 60

Gly Ile Ala Pro Ser Glu Ile Asp Asn Ala Phe Lys Gln Ala Arg Asp

65 70 75 80

Ser Met Glu Ser Asn Asp Glu Leu Ile Ala Leu Val Arg Glu Leu Lys

85 90 95

Thr Gln Leu Asp Gly Glu Leu Leu Val Phe Ala Leu Ser Asn Ile Ser

100 105 110

Leu Pro Asp Tyr Glu Tyr Val Leu Thr Lys Pro Ala Asp Trp Ser Ile

115 120 125

Phe Asp Lys Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys Pro His

130 135 140

Leu Gly Val Tyr Lys His Val Ile Ala Glu Thr Gly Ile Asp Pro Arg

145 150 155 160

Thr Thr Val Phe Val Asp Asp Lys Ile Asp Asn Val Leu Ser Ala Arg

165 170 175

Ser Val Gly Met His Gly Ile Val Phe Glu Lys Gln Glu Asp Val Met

180 185 190

Arg Ala Leu Arg Asn Ile Phe Gly Asp Pro Val Arg Arg Gly Arg Glu

195 200 205

Tyr Leu Arg Arg Asn Ala Met Arg Leu Glu Ser Val Thr Asp His Gly

210 215 220

Val Ala Phe Gly Glu Asn Phe Thr Gln Leu Leu Ile Leu Glu Leu Thr

225 230 235 240

Asn Asp Pro Ser Leu Val Thr Leu Pro Asp Arg Pro Arg Thr Trp Asn

245 250 255

Phe Phe Arg Gly Asn Gly Gly Arg Pro Ser Lys Pro Leu Phe Ser Glu

260 265 270

Ala Phe Pro Asp Asp Leu Asp Thr Thr Ser Leu Ala Leu Thr Val Leu

275 280 285

Gln Arg Asp Pro Gly Val Ile Ser Ser Val Met Asp Glu Met Leu Asn

290 295 300

Tyr Arg Asp Pro Asp Gly Ile Met Gln Thr Tyr Phe Asp Asp Gly Arg

305 310 315 320

Gln Arg Leu Asp Pro Phe Val Asn Val Asn Val Leu Thr Phe Phe Tyr

325 330 335

Thr Asn Gly Arg Gly His Glu Leu Asp Gln Cys Leu Thr Trp Val Arg

340 345 350

Glu Val Leu Leu Tyr Arg Ala Tyr Leu Gly Gly Ser Arg Tyr Tyr Pro

355 360 365

Ser Ala Asp Cys Phe Leu Tyr Phe Ile Ser Arg Leu Phe Ala Cys Thr

370 375 380

Asn Asp Pro Val Leu His His Gln Leu Lys Pro Leu Phe Val Glu Arg

385 390 395 400

Val Gln Glu Gln Ile Gly Val Glu Gly Asp Ala Leu Glu Leu Ala Phe

405 410 415

Arg Leu Leu Val Cys Ala Ser Leu Asp Val Gln Asn Ala Ile Asp Met

420 425 430

Arg Arg Leu Leu Glu Met Gln Cys Glu Asp Gly Gly Trp Glu Gly Gly

435 440 445

Asn Leu Tyr Arg Phe Gly Thr Thr Gly Leu Lys Val Thr Asn Arg Gly

450 455 460

Leu Thr Thr Ala Ala Ala Val Gln Ala Ile Glu Ala Ser Gln Arg Arg

465 470 475 480

Pro Pro Ser Pro Ser Pro Ser Val Glu Ser Thr Lys Ser Pro Ile Thr

485 490 495

Pro Val Thr Pro Met Leu Glu Val Pro Ser Leu Gly Leu Ser Ile Ser

500 505 510

Arg Pro Ser Ser Pro Leu Leu Gly Tyr Phe Arg Leu Pro Trp Lys Lys

515 520 525

Ser Ala Glu Val His

530

<210> 50

<211> 1602

<212> DNA

<213> 弯孢胶化孔菌(Gelatoporia subvermispora)_EMD37666.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 50

atgtccgcgg cagctcaata cacgaccctc attctcgacc ttggcgacgt cctgttcacc 60

tggtcaccga aaaccaagac gagcatcccc cctcggactc tgaaggagat tctcaattcc 120

gcgacatggt atgagtatga gcgcggccgc atctctcagg acgaatgtta cgaacgcgtt 180

ggcacggagt tcggaatcgc gcctagcgaa atcgacaacg cgttcaagca agctcgggat 240

tccatggaat ccaacgacga actgatcgcc cttgttcggg aactcaagac gcagttggac 300

ggcgaactcc ttgtcttcgc actctcaaat atctcgttgc ctgactacga gtacgtcctc 360

acgaaaccgg ccgactggtc catcttcgac aaggtcttcc cttccgccct cgtgggcgag 420

cgcaagccgc acctcggcgt ttacaagcac gtcattgcag aaacgggcat tgatccgcga 480

accaccgttt tcgtggacga caagatcgac aacgtgctct cagcgcggtc tgtaggtatg 540

catgggatcg ttttcgagaa gcaggaagac gtaatgcgcg ctctccgaaa catcttcgga 600

gacccggttc ggcgagggcg cgagtacttg cgccgtaatg ccatgaggct tgaatcggtt 660

acagaccatg gtgtggcgtt tggcgagaac ttcacacaac tccttatcct cgaactaacg 720

aacgatccct ccctcgttac gctccctgat cgtcctcgaa catggaattt cttccgaggt 780

aacgggggac gaccaagcaa accattattc tcggaggcct tccccgatga cttggacact 840

acttcactag cgttgactgt cctccaaaga gatcccggcg tcatctcttc tgtgatggac 900

gaaatgttga actacaggga tccggacggc attatgcaga catacttcga cgatggtcgg 960

caaagactcg atccatttgt caatgtcaat gtcttaacgt tcttctacac caacggacgt 1020

ggtcatgaac tggaccaatg ccttacatgg gtccgcgaag ttttgctcta tcgcgcctat 1080

ctcggcggct cacgttatta cccctccgcc gactgctttc tctacttcat cagccgcctt 1140

ttcgcatgca cgaatgaccc cgtgctacac caccaactca aaccgctctt cgtcgagcgc 1200

gtgcaggagc aaatcggcgt ggagggcgat gcgctcgagt tggcgttccg attgctcgtc 1260

tgtgcaagcc tggacgtcca aaacgcgatc gacatgcgca ggctgctcga gatgcaatgc 1320

gaagatggcg gctgggaggg cgggaacctt tataggtttg gcacgaccgg gctcaaggtg 1380

actaaccggg gcctgacgac tgcagcggcc gtacaggcca tcgaggcgtc ccaacggcgc 1440

ccaccatcac cgtccccctc cgtcgaatct acaaagagcc caataacccc tgtgacgccc 1500

atgctggagg tcccctcgct cggcctgagc atctcgaggc cgtccagccc tttactcggc 1560

tacttcaggc tcccgtggaa gaagtcggcc gaagtacact ga 1602

<210> 51

<211> 1602

<212> DNA

<213> 人工序列_EMD37666.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 51

atgtctgcgg cggctcaata cacgactttg attctggatc tgggtgatgt tctgttcact 60

tggtccccga aaaccaagac cagcatccct ccgcgtaccc tgaaagaaat cctgaatagc 120

gctacctggt atgagtacga gcgtggtcgc atttcccaag acgagtgtta cgaacgtgtg 180

ggcaccgagt tcggcattgc gccgagcgag attgacaacg cgttcaaaca agcgcgcgat 240

tcgatggaaa gcaatgatga actgatcgca ctggtccgtg agctgaaaac gcagctggac 300

ggtgagctgc tggttttcgc actgtccaat attagcctgc cggattacga atacgtcttg 360

accaaaccgg cggactggag catctttgac aaagtgttcc ctagcgcctt ggtgggcgag 420

cgtaagccgc atctgggcgt ttataaacac gttattgcgg aaacgggcat tgatccgcgc 480

acgacggttt tcgtggacga caagattgac aatgtgttaa gcgcacgcag cgtcggtatg 540

catggtatcg tgtttgagaa acaagaagat gtcatgcgtg cactgcgtaa catctttggt 600

gatccggtcc gtcgtggtcg tgagtatctg cgtagaaacg caatgcgtct ggagtccgtg 660

accgaccacg gcgtggcgtt tggtgagaac tttacccagt tgctgattct ggaattgacg 720

aacgacccga gcctggtcac cctgcctgat cgtccgcgta cctggaactt ttttcgcggc 780

aatggtggcc gcccgagcaa gccgctgttc agcgaagcgt tcccggatga tctggatacc 840

acgagcctgg cgctgaccgt gctgcagcgc gacccgggtg ttatcagcag cgttatggac 900

gaaatgctga attaccgtga cccggacggt atcatgcaga cttatttcga tgacggtcgc 960

caacgcttgg acccatttgt gaacgtcaat gttctgacct ttttctatac gaacggccgt 1020

ggtcacgaac tggaccagtg tctgacgtgg gtgcgtgaag tcctcttgta tcgtgcgtac 1080

cttggtggct cacgctacta cccatcggcg gattgcttcc tgtacttcat ctctcgtctg 1140

tttgcgtgta ccaatgaccc ggtgctgcac catcagctga agccactgtt tgttgagcgt 1200

gtccaagagc aaattggtgt cgagggtgat gcactggaac tggcttttcg tctgctggtc 1260

tgcgccagcc tggatgtcca gaatgccatc gacatgcgcc gtctgctgga aatgcagtgc 1320

gaagatggcg gttgggaggg tggtaacctc taccgcttcg gcaccacggg cctgaaagtt 1380

accaaccgcg gtctgacgac cgcagccgcc gttcaagcga tcgaagcgag ccaacgccgt 1440

ccgccgagcc cgagcccgtc tgtagagagc acgaaaagcc cgattacccc ggtgaccccg 1500

atgctggaag ttccaagcct gggcttatct atcagccgtc cgtccagccc gctgctgggt 1560

tatttccgtt tgccgtggaa gaaaagcgca gaagtgcact aa 1602

<210> 52

<211> 527

<212> PRT

<213> 弯孢胶化孔菌(Gelatoporia subvermispora)_EMD37666-B蛋白

<400> 52

Met Ser Ala Ala Ala Gln Tyr Thr Thr Leu Ile Leu Asp Leu Gly Asp

1 5 10 15

Val Leu Phe Thr Trp Ser Pro Lys Thr Lys Thr Ser Ile Pro Pro Arg

20 25 30

Thr Leu Lys Glu Ile Leu Asn Ser Ala Thr Trp Tyr Glu Tyr Glu Arg

35 40 45

Gly Arg Ile Ser Gln Asp Glu Cys Tyr Glu Arg Val Gly Thr Glu Phe

50 55 60

Gly Ile Ala Pro Ser Glu Ile Asp Asn Ala Phe Lys Gln Ala Arg Asp

65 70 75 80

Ser Met Glu Ser Asn Asp Glu Leu Ile Ala Leu Val Arg Glu Leu Lys

85 90 95

Thr Gln Leu Asp Gly Glu Leu Leu Val Phe Ala Leu Ser Asn Ile Ser

100 105 110

Leu Pro Asp Tyr Glu Tyr Val Leu Thr Lys Pro Ala Asp Trp Ser Ile

115 120 125

Phe Asp Lys Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys Pro His

130 135 140

Leu Gly Val Tyr Lys His Val Ile Ala Glu Thr Gly Ile Asp Pro Arg

145 150 155 160

Thr Thr Val Phe Val Asp Asp Lys Ile Asp Asn Val Leu Ser Ala Arg

165 170 175

Ser Val Gly Met His Gly Ile Val Phe Glu Lys Gln Glu Asp Val Met

180 185 190

Arg Ala Leu Arg Asn Ile Phe Gly Asp Pro Val Arg Arg Gly Arg Glu

195 200 205

Tyr Leu Arg Arg Asn Ala Met Arg Leu Glu Ser Val Thr Asp His Gly

210 215 220

Val Ala Phe Gly Glu Asn Phe Thr Gln Leu Leu Ile Leu Glu Leu Thr

225 230 235 240

Asn Asp Pro Ser Leu Val Thr Leu Pro Asp Arg Pro Arg Thr Trp Asn

245 250 255

Phe Phe Arg Gly Lys Pro Leu Phe Ser Glu Ala Phe Pro Asp Asp Leu

260 265 270

Asp Thr Thr Ser Leu Ala Leu Thr Val Leu Gln Arg Asp Pro Gly Val

275 280 285

Ile Ser Ser Val Met Asp Glu Met Leu Asn Tyr Arg Asp Pro Asp Gly

290 295 300

Ile Met Gln Thr Tyr Phe Asp Asp Gly Arg Gln Arg Leu Asp Pro Phe

305 310 315 320

Val Asn Val Asn Val Leu Thr Phe Phe Tyr Thr Asn Gly Arg Gly His

325 330 335

Glu Leu Asp Gln Cys Leu Thr Trp Val Arg Glu Val Leu Leu Tyr Arg

340 345 350

Ala Tyr Leu Gly Gly Ser Arg Tyr Tyr Pro Ser Ala Asp Cys Phe Leu

355 360 365

Tyr Phe Ile Ser Arg Leu Phe Ala Cys Thr Asn Asp Pro Val Leu His

370 375 380

His Gln Leu Lys Pro Leu Phe Val Glu Arg Val Gln Glu Gln Ile Gly

385 390 395 400

Val Glu Gly Asp Ala Leu Glu Leu Ala Phe Arg Leu Leu Val Cys Ala

405 410 415

Ser Leu Asp Val Gln Asn Ala Ile Asp Met Arg Arg Leu Leu Glu Met

420 425 430

Gln Cys Glu Asp Gly Gly Trp Glu Gly Gly Asn Leu Tyr Arg Phe Gly

435 440 445

Thr Thr Gly Leu Lys Val Thr Asn Arg Gly Leu Thr Thr Ala Ala Ala

450 455 460

Val Gln Ala Ile Glu Ala Ser Gln Arg Arg Pro Pro Ser Pro Ser Pro

465 470 475 480

Ser Val Glu Ser Thr Lys Ser Pro Ile Thr Pro Val Thr Pro Met Leu

485 490 495

Glu Val Pro Ser Leu Gly Leu Ser Ile Ser Arg Pro Ser Ser Pro Leu

500 505 510

Leu Gly Tyr Phe Arg Leu Pro Trp Lys Lys Ser Ala Glu Val His

515 520 525

<210> 53

<211> 1584

<212> DNA

<213> 人工序列_EMD37666-B 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 53

atgtctgcgg ctgctcaata tactactttg attctggatc tgggcgacgt tctgttcacg 60

tggagcccga aaaccaagac cagcattcca ccgcgtaccc tgaaggagat cctcaatagc 120

gcgacttggt acgagtatga gcgtggccgc atcagccaag acgagtgcta cgaacgcgtc 180

ggtacggaat ttggcattgc accaagcgag attgacaatg cgtttaaaca agcgcgtgac 240

agcatggaaa gcaatgacga actgatcgcg ctggtccgtg agctgaaaac ccagctggat 300

ggtgagctgt tggtgtttgc gctgtcgaac atctctctgc cggactacga gtatgttctg 360

accaaaccgg cggattggag catttttgat aaagtgtttc cgagcgcgct ggttggtgag 420

cgcaagccgc acctgggtgt gtacaaacac gttattgcag agactggcat cgacccgcgt 480

acgacggttt tcgttgacga caagatcgat aacgttctga gcgcacgtag cgtcggtatg 540

cacggtattg ttttcgaaaa acaagaagat gttatgcgcg cactgcgtaa tatcttcggc 600

gatccggtca gacgtggccg tgagtatctg cgccgcaatg cgatgcgtct ggaatcggtg 660

accgatcatg gtgtcgcctt tggcgagaat ttcacccagc tgctgatttt agagctgacc 720

aatgatccta gcctggtgac gctgccggat cgtccgcgta cctggaactt tttccgcggc 780

aagccgttgt tctccgaagc cttcccggac gacctggaca cgaccagcct ggcgctgacc 840

gtgctgcaac gtgatccggg tgtgatctct tccgtaatgg acgaaatgct gaactaccgt 900

gacccggacg gtatcatgca gacctatttt gacgacggtc gtcagcgtct ggacccgttt 960

gtgaacgtga atgtcctgac gttcttttac accaatggtc gcggtcacga actggatcag 1020

tgtctgacct gggtccgcga agtgctgctg tatcgtgcat acctgggtgg cagccgttat 1080

tacccgagcg ccgattgctt tctgtacttt atcagccgtc tgttcgcgtg cacgaacgat 1140

ccggttctgc atcaccagct gaagccgtta tttgttgagc gcgttcagga acaaattggt 1200

gtcgagggtg atgcgctgga attggcattc cgcctgttgg tctgcgccag ccttgatgtc 1260

cagaacgcca ttgacatgcg tcgcttgctc gaaatgcagt gtgaggacgg cggttgggag 1320

ggtggcaacc tgtaccgttt cggtacgacc ggcctgaaag tcacgaaccg tggtctgacg 1380

acggcagctg cggtgcaagc aattgaagcc agccaacgtc gtccgccatc cccgtcaccg 1440

agcgttgagt ccaccaagag cccgattacc cctgtgaccc cgatgcttga agttccgagc 1500

ctgggtctga gcatctcccg tcctagcagc ccgctgttgg gttacttccg cctgccgtgg 1560

aagaaaagcg ctgaggtgca ttaa 1584

<210> 54

<211> 486

<212> PRT

<213> 土曲霉(Aspergillus terreus)_XP_001217376.1蛋白

<400> 54

Met Ala Ile Thr Lys Gly Pro Val Lys Ala Leu Ile Leu Asp Phe Ser

1 5 10 15

Asn Val Leu Cys Ser Trp Lys Pro Pro Ser Asn Val Ala Val Pro Pro

20 25 30

Gln Ile Leu Lys Met Ile Met Ser Ser Asp Ile Trp His Asp Tyr Glu

35 40 45

Cys Gly Arg Tyr Ser Arg Glu Asp Cys Tyr Ala Arg Val Ala Asp Arg

50 55 60

Phe His Ile Ser Ala Ala Asp Met Glu Asp Thr Leu Lys Gln Ala Arg

65 70 75 80

Lys Ser Leu Gln Val His His Glu Thr Leu Leu Phe Ile Gln Gln Val

85 90 95

Lys Lys Asp Ala Gly Gly Glu Leu Met Val Cys Gly Met Thr Asn Thr

100 105 110

Pro Arg Pro Glu Gln Asp Val Met His Ser Ile Asn Ala Glu Tyr Pro

115 120 125

Val Phe Asp Arg Ile Tyr Ile Ser Gly Leu Met Gly Met Arg Lys Pro

130 135 140

Ser Ile Cys Phe Tyr Gln Arg Val Met Glu Glu Ile Gly Leu Ser Gly

145 150 155 160

Asp Ala Ile Met Phe Ile Asp Asp Lys Leu Glu Asn Val Ile Ala Ala

165 170 175

Gln Ser Val Gly Ile Arg Gly Val Leu Phe Gln Ser Gln Gln Asp Leu

180 185 190

Arg Arg Val Val Leu Asn Phe Leu Gly Asp Pro Val His Arg Gly Leu

195 200 205

Gln Phe Leu Ala Ala Asn Ala Lys Lys Met Asp Ser Val Thr Asn Thr

210 215 220

Gly Asp Thr Ile Gln Asp Asn Phe Ala Gln Leu Leu Ile Leu Glu Leu

225 230 235 240

Ala Gln Asp Arg Glu Leu Val Lys Leu Gln Ala Gly Lys Arg Thr Trp

245 250 255

Asn Tyr Phe Ile Gly Pro Pro Lys Leu Thr Thr Ala Thr Phe Pro Asp

260 265 270

Asp Met Asp Thr Thr Ser Met Ala Leu Ser Val Leu Pro Val Ala Glu

275 280 285

Asp Val Val Ser Ser Val Leu Asp Glu Met Leu Lys Phe Val Thr Asp

290 295 300

Asp Gly Ile Phe Met Thr Tyr Phe Asp Ser Ser Arg Pro Arg Val Asp

305 310 315 320

Pro Val Val Cys Ile Asn Val Leu Gly Val Phe Cys Arg His Asn Arg

325 330 335

Glu Arg Asp Val Leu Pro Thr Phe His Trp Ile Arg Asp Ile Leu Ile

340 345 350

Asn Arg Ala Tyr Leu Ser Gly Thr Arg Tyr Tyr Pro Ser Pro Asp Leu

355 360 365

Phe Leu Phe Phe Leu Ala Arg Leu Cys Leu Ala Val Arg Asn Gln Ser

370 375 380

Leu Arg Glu Gln Leu Val Leu Pro Leu Val Asp Arg Leu Arg Glu Arg

385 390 395 400

Val Gly Ala Pro Gly Glu Ala Val Ser Leu Ala Ala Arg Ile Leu Ala

405 410 415

Cys Arg Ser Phe Gly Ile Asp Ser Ala Arg Asp Met Asp Ser Leu Arg

420 425 430

Gly Lys Gln Cys Glu Asp Gly Gly Trp Pro Val Glu Trp Val Tyr Arg

435 440 445

Phe Ala Ser Phe Gly Leu Asn Val Gly Asn Arg Gly Leu Ala Thr Ala

450 455 460

Phe Ala Val Arg Ala Leu Glu Ser Pro Tyr Gly Glu Ser Ala Val Lys

465 470 475 480

Val Met Arg Arg Ile Val

485

<210> 55

<211> 1461

<212> DNA

<213> 土曲霉(Aspergillus terreus)_XP_001217376.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 55

atggctatca ccaagggtcc agttaaggcg cttattcttg acttttccaa tgttctctgc 60

tcgtggaagc ctcccagcaa tgttgcggtg ccgccccaga tactcaaaat gatcatgtcc 120

tctgacatat ggcatgacta cgagtgcgga cggtactcga gagaggactg ctatgccaga 180

gtggcagacc gttttcatat cagcgccgcg gacatggaag acacgctgaa acaggcgcgc 240

aagagcctgc aggttcacca tgagacactg ttgtttatcc agcaagtcaa gaaggatgcc 300

gggggcgagt tgatggtgtg tgggatgacc aacacgcccc ggccagagca agacgtaatg 360

cattcaatca acgcggagta tcctgtgttt gataggatat atatatccgg tctcatgggc 420

atgaggaagc cgagcatctg cttctaccag cgggtgatgg aggagattgg cctatcaggc 480

gatgcgatca tgtttataga tgacaagttg gagaatgtca tcgccgccca gtcggtaggg 540

atccgaggcg ttctatttca gagtcagcaa gatctccgtc gggttgtatt aaatttcttg 600

ggcgatccgg tccatcgcgg cctgcagttc ctagcggcca atgcgaaaaa gatggatagt 660

gtgaccaaca ccggcgatac tatccaagat aattttgctc agctcctcat cttggagctg 720

gcccaggaca gggaattggt gaagcttcag gctggaaaaa ggacttggaa ttacttcata 780

gggcctccca agctcacaac agccacgttc cccgatgaca tggacaccac atctatggct 840

ctctcggtcc ttcctgtggc cgaggatgtg gtctcttctg tcctggatga gatgcttaaa 900

ttcgtcaccg atgacggtat ctttatgact tacttcgatt cctcgcgccc tcgagtcgac 960

ccagtcgtat gtatcaacgt cttgggtgtt ttctgcaggc ataaccgaga gcgagacgtc 1020

cttccaacgt tccattggat tcgagacatc ctgatcaacc gggcatatct ctcgggcacc 1080

cgatactacc catcgcccga tttgtttttg tttttccttg cacgcctctg cctggcagtc 1140

cggaatcaga gcctacggga acaacttgtc ttgcctctgg tagaccgact gcgtgagcgg 1200

gtgggcgcac ctggagaagc ggtctcattg gcagcgcgga tccttgcctg ccgtagcttt 1260

ggtatcgaca gtgcgagaga catggacagc ttgaggggaa aacaatgcga ggatggcggc 1320

tggccagtgg agtgggttta ccggtttgcc tctttcggcc tgaacgtagg caatcggggt 1380

cttgctactg ccttcgcggt cagggcgctc gaaagcccct atggtgagtc ggcggtgaag 1440

gttatgagac gcatcgtctg a 1461

<210> 56

<211> 1461

<212> DNA

<213> 人工序列_XP_001217376.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 56

atggcaatca ctaagggccc agttaaagcg ctgattcttg atttttctaa cgttctgtgt 60

agctggaagc cgccgagcaa tgttgcggtc ccgcctcaaa ttctgaagat gattatgtcg 120

agcgacatct ggcatgatta tgagtgtggc cgttacagcc gtgaggactg ctacgcccgt 180

gttgctgacc gttttcatat cagcgcagcg gacatggaag ataccctgaa acaggcacgt 240

aagtccctgc aagtgcacca cgaaacgctg ctgttcatcc aacaggtgaa gaaagacgcg 300

ggtggtgagc tgatggtttg cggcatgacc aacacgccgc gtccggaaca agacgtgatg 360

cattccatca atgctgagta tccggtgttc gaccgtattt acattagcgg cctgatgggc 420

atgcgtaaac cgagcatttg tttctaccaa cgcgtaatgg aagagattgg tctgagcggt 480

gacgccatca tgttcattga cgataaactg gaaaatgtga ttgccgcaca gagcgtgggt 540

atccgcggtg tgctgttcca aagccagcaa gatctgcgtc gtgtcgtgct gaactttctg 600

ggcgatccgg tccaccgtgg tctgcagttc ttggcggcga acgcaaagaa aatggacagc 660

gtcacgaata ccggcgacac tatccaagac aatttcgcac agctgttgat cttagagctg 720

gcgcaggatc gcgaattggt gaaattgcag gccggtaaac gtacctggaa ctactttatt 780

ggtccgccga agctgaccac ggcgacgttt ccggatgata tggacacgac cagcatggcg 840

ctgtcggtgc tgcctgtcgc ggaagatgtc gtgagctctg ttctggacga gatgctgaag 900

ttcgtgaccg atgatggtat ctttatgacc tatttcgact ctagccgtcc gcgtgtcgat 960

ccggttgtct gcattaatgt gttgggtgtt ttctgccgcc acaatcgtga gcgcgacgtg 1020

ttgccgacct ttcactggat tcgtgatatt ctgatcaacc gcgcatatct gagcggcacg 1080

cgctattacc cgtccccgga tctgtttctg tttttcctgg ctcgtctgtg cctggccgtt 1140

cgcaaccaga gcctgcgcga acaactggtt ctcccgctgg ttgatcgtct gcgcgagcgt 1200

gttggtgctc cgggtgaggc tgtgagcctg gcggcacgta tcctggcgtg ccgtagcttc 1260

ggtatcgact cagcccgcga catggactcc ttgcgtggca aacagtgtga agatggtggt 1320

tggccggtcg aatgggtcta tcgcttcgcg agctttggtc tgaacgttgg caaccgtggt 1380

ttggccaccg cgtttgcggt tagagcgctg gagtccccat acggcgagag cgcagttaag 1440

gttatgcgcc gtatcgtgta a 1461

<210> 57

<211> 483

<212> PRT

<213> 棘孢曲霉(Aspergillus aculeatus)_OJJ98394.1蛋白

<400> 57

Met Pro Ser Val Lys Ala Leu Val Leu Asp Phe Ala Gly Val Leu Cys

1 5 10 15

Ser Trp Thr Pro Pro Ala Glu Ser Pro Leu Ser Pro Ala Gln Leu Lys

20 25 30

Gln Leu Met Ser Ser Glu Ile Trp Phe Glu Tyr Glu Arg Gly Arg Tyr

35 40 45

Ser Glu Glu Glu Cys Tyr Ala Lys Leu Val Glu Arg Phe Ser Ile Ser

50 55 60

Ala Ala Asp Met Ala Ser Thr Met Glu Gln Ala Arg Gln Ser Leu Glu

65 70 75 80

Leu Asn His Ala Val Leu Gln Leu Val Ser Glu Ile Arg Lys Arg Asn

85 90 95

Pro Gly Leu Lys Val Tyr Gly Met Thr Asn Thr Pro His Ala Glu Gln

100 105 110

Asp Cys Val Asn Arg Ile Val Asn Ser Tyr Pro Val Phe Asp His Val

115 120 125

Tyr Leu Ser Gly Leu Val Gly Met Arg Lys Pro Asp Leu Gly Phe Tyr

130 135 140

Arg Phe Val Leu Ala Glu Thr Gly Leu Arg Pro Asp Glu Val Val Phe

145 150 155 160

Val Asp Asp Lys Thr Glu Asn Val Leu Val Ala Gln Ser Val Gly Met

165 170 175

His Gly Val Val Phe Gln Asn Val Thr Asp Phe Lys Gln Gln Ile Ile

180 185 190

Asn Val Thr Gly Asp Pro Val Ser Arg Gly Leu Arg Tyr Leu Arg Ser

195 200 205

Asn Ala Lys Ser Leu Leu Thr Val Thr Ser Asn Asn Ser Val Ile His

210 215 220

Glu Asn Phe Ala Gln Leu Leu Ile Leu Glu Leu Thr Gly Asp Arg Asp

225 230 235 240

Leu Ile Glu Leu Glu Pro Trp Asp Arg Thr Trp Asn Tyr Phe Ile Gly

245 250 255

Val Pro Gln Ser Pro Thr Ser Thr Phe Pro Asn Asp Leu Asp Thr Thr

260 265 270

Ser Ile Ala Leu Ser Val Leu Pro Ile His Lys Asp Val Val Ala Asp

275 280 285

Val Met Asp Glu Ile Met Leu Leu Leu Asp Asn Asp Gly Ile Val Pro

290 295 300

Thr Tyr Phe Asp Pro Thr Arg Pro Arg Val Asp Pro Val Val Cys Val

305 310 315 320

Asn Val Leu Ser Leu Phe Ala Gln Asn Gly Arg Glu Ser Glu Leu Leu

325 330 335

Ala Thr Phe Asn Trp Val Leu Asp Val Leu Arg His Arg Ala Tyr Leu

340 345 350

Gln Gly Thr Arg Tyr Tyr Ile Ser Pro Asp Ala Phe Leu Tyr Phe Leu

355 360 365

Ala Arg Leu Ser Val Phe Leu Arg Met Ser Pro Leu Arg Ala Arg Leu

370 375 380

Met Pro Leu Leu Glu Glu Arg Val Tyr Glu Arg Ile Gly Ala His Gly

385 390 395 400

Asp Ala Ile Ser Leu Ala Met Arg Ile Tyr Thr Cys Lys Leu Leu Gly

405 410 415

Met Ser Asn Met Leu Asp Glu Arg Ala Leu Arg Asp Met Gln Cys Glu

420 425 430

Asp Gly Gly Phe Pro Thr Ser Trp Val Tyr Arg Phe Gly Ser Thr Gly

435 440 445

Val Lys Ile Gly Asn Arg Gly Leu Thr Thr Ala Leu Ala Ile Lys Ala

450 455 460

Ile Glu Met Pro Leu Ala Ser Leu Trp Lys Ser Trp Gly Leu Thr Thr

465 470 475 480

Asp Ile Arg

<210> 58

<211> 1452

<212> DNA

<213> 棘孢曲霉(Aspergillus aculeatus)_OJJ98394.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 58

atgccctccg tcaaagcact ggtcctggac ttcgccggag ttctatgctc atggaccccg 60

ccagccgaga gcccgctctc cccagcccag ctcaaacaac tcatgtcctc cgagatatgg 120

ttcgaatacg agcgcgggag atattccgaa gaagaatgtt atgcgaagct cgtcgaacgg 180

ttctccatca gcgctgcgga catggcttcc accatggaac aggcccgtca gagcctggaa 240

ctgaaccacg ccgtacttca gcttgtcagc gagataagga agcggaaccc cgggctcaaa 300

gtttatggca tgacgaacac gccccatgcg gaacaggatt gtgtgaatcg catcgtgaac 360

agctatcctg ttttcgacca tgtgtatctc tccgggctcg ttgggatgcg caaaccagat 420

cttggattct atcggtttgt tctcgcagag accgggttga ggcctgacga ggtcgtgttc 480

gtcgacgaca aaacggagaa tgtgttggtc gcgcagtccg tggggatgca cggcgtggtg 540

ttccagaacg ttacggattt caagcagcag atcataaacg tgacgggaga ccctgtctct 600

cggggcttga ggtatctccg ctcgaatgca aagagcctcc tcactgtgac tagcaataac 660

tccgtgatcc acgaaaactt tgcgcagttg ctgattctgg agctgacggg cgaccgagac 720

ttgatcgaac tcgagccttg ggatcgaaca tggaactact tcatcggggt tcctcagtcg 780

ccgacgagca ccttccccaa cgacctggac accacctcta tcgcgctctc ggtccttccc 840

attcataagg acgtcgttgc cgatgtgatg gacgagatta tgcttctcct agacaacgac 900

gggatagtcc caacatattt tgatcccact cgccctcgag tcgacccagt cgtgtgtgtg 960

aatgtactca gcctgtttgc ccaaaacggc cgagaatccg agttactcgc caccttcaac 1020

tgggtgctgg acgtgctgcg acatagagcc tacctgcagg gcacgagata ttacatcagt 1080

ccggacgcct tcttgtactt tctagccaga ctctcggtct ttctgaggat gagtccactc 1140

cgcgctcggc taatgcctct cctggaagaa agagtgtatg agcgaattgg tgcccatggc 1200

gacgccattt cgctggctat gcggatctat acgtgtaagc tgctcgggat gtcgaatatg 1260

ctcgatgaaa gagcattgcg ggacatgcag tgtgaggatg gcggcttccc tacaagttgg 1320

gtctatagat ttggatcgac cggagtgaag attgggaaca gggggttgac tactgcactt 1380

gcaataaagg ccattgagat gcctctcgct tcgctttgga agtcgtgggg attgacgact 1440

gacattcgat aa 1452

<210> 59

<211> 1452

<212> DNA

<213> 人工序列_OJJ98394.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 59

atgccgtcgg ttaaagcgtt ggttctggat tttgcgggtg tgttgtgttc ttggactcca 60

ccggcggaaa gcccgttgtc cccagcgcag ctgaagcagc tgatgagcag cgagatctgg 120

tttgagtatg agcgtggccg ctatagcgaa gaagagtgtt atgcaaaatt ggtggagcgt 180

ttctctatct cggccgcaga tatggcgagc acgatggaac aggcccgtca atcgctggag 240

ttgaaccacg ccgtgctgca attagtttcc gagattcgta aacgtaatcc gggcttaaag 300

gtttacggta tgactaatac cccgcatgca gagcaagatt gtgtgaaccg tattgtcaat 360

agctatccgg tttttgatca tgtctacctg agcggtctgg tgggtatgcg caaaccggat 420

ctgggctttt accgtttcgt tctggcagag actggtctgc gcccggatga agtcgtgttc 480

gttgacgaca agaccgaaaa tgtcctggtg gctcaatccg ttggcatgca tggtgtggtg 540

ttccaaaatg taaccgactt caaacaacag attatcaatg tcacgggtga tcctgtcagc 600

cgtggtttgc gctacttgcg ttccaacgcg aagtctctgc tcactgttac cagcaataac 660

agcgttatcc atgagaattt cgcgcagctg ctgatcctgg aactgacggg cgaccgtgac 720

ctgattgaac tggaaccgtg ggaccgtacg tggaactact ttatcggcgt gccgcaaagc 780

ccgaccagca cctttccgaa cgacctggat acgaccagca ttgccctgag cgttctgccg 840

attcacaaag atgtggttgc ggacgtgatg gatgagatta tgctgctgct ggacaatgac 900

ggtattgtcc cgacctactt cgatccaacc cgtccgcgtg ttgatcctgt tgtgtgcgtc 960

aacgttctga gcctgttcgc acagaacggt cgcgagtccg aattgctggc gacgttcaac 1020

tgggttttgg acgttctgag acaccgtgcg tatttgcagg gtacgcgcta ttatatcagc 1080

ccggatgcct ttctgtattt tctggcgcgc ctgtctgtgt ttctgcgtat gtctccgttg 1140

cgcgctcgtc tgatgccgct gctggaagaa cgcgtttatg agcgtatcgg cgcacacggc 1200

gatgctatta gcctggcgat gcgcatttac acctgtaagc tgctgggcat gagcaatatg 1260

ctggacgagc gtgcactgcg tgacatgcag tgtgaagatg gtggtttccc aaccagctgg 1320

gtgtaccgtt ttggtagcac gggcgtgaaa attggtaacc gtggcttgac gaccgcactg 1380

gccattaagg ccatcgaaat gccgctggcc agcctttgga aaagctgggg cctgaccacc 1440

gatattcgct aa 1452

<210> 60

<211> 485

<212> PRT

<213> 乌达加瓦曲霉(Aspergillus udagawae)_GAO87501.1蛋白

<400> 60

Met Thr Arg Gln Lys Ser Pro Gln Tyr Lys Ala Ile Ile Phe Asp Leu

1 5 10 15

Gly Asp Val Phe Phe Thr Trp Asp Ala Pro Lys Asp Thr Ala Val Leu

20 25 30

Pro Asn Leu Phe Lys Lys Met Leu Thr Ser Pro Thr Trp Ser Asp Tyr

35 40 45

Glu Arg Gly Lys Leu Ser Glu Glu Ser Cys Tyr Glu Arg Leu Ala Glu

50 55 60

Gln Phe Asp Val Asp Ser Ser Glu Ile Ala Arg Ser Leu Arg Lys Ala

65 70 75 80

Gln Gln Ser Leu Thr Thr Asp Ala Ala Ile Val Ser Leu Ile Ser Glu

85 90 95

Ile Arg Ala Leu Ala Gly His Ile Ala Ile Tyr Ala Met Ser Asn Ile

100 105 110

Ser Ala Pro Ala Tyr Ala Ala Val Leu Gln Thr Gln Pro Glu Met Gly

115 120 125

Ile Phe Asp Gly Val Phe Pro Ser Gly Cys Tyr Gly Thr Arg Lys Pro

130 135 140

Glu Leu Leu Phe Tyr Lys Lys Val Leu Gln Glu Ile Ala Val Pro Pro

145 150 155 160

Asn Gln Ile Ile Phe Ile Asp Asp Gln Leu Glu Asn Val Val Ser Ala

165 170 175

Gln Ser Thr Gly Met His Gly Ile Val Tyr Thr Gly Ala Gly Glu Leu

180 185 190

Ser Arg Gln Leu Arg Asn Leu Val Leu Asp Pro Val Gln Arg Gly Arg

195 200 205

Glu Phe Leu Arg Arg Asn Ala Gly Ala Leu Tyr Ser Ile Cys Glu Thr

210 215 220

Gly Gln Val Ile Arg Glu Asn Phe Ser Gln Leu Leu Ile Leu Glu Ala

225 230 235 240

Thr Gly Asp Arg Ser Leu Val Asn Leu Glu Tyr Gln Gln Arg Ser Trp

245 250 255

Asn Phe Phe Gln Gly Gly Pro Pro Ser Thr Ser Glu Thr Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Ile Ala Leu Met Ile Leu Pro Ala Asp Asp

275 280 285

Asn Thr Val Asn Ser Val Leu Gly Glu Ile Ser Glu Val Ala Asn Asp

290 295 300

Glu Gly Ile Val Asn Thr Tyr Phe Asp Gln Thr Arg Gln Arg Ile Asp

305 310 315 320

Pro Ala Val Cys Val Asn Val Leu Arg Leu Phe Tyr Thr Tyr Gly Arg

325 330 335

Gly Ala Thr Leu Pro Leu Thr Leu Gln Trp Val Ser Asp Val Leu Glu

340 345 350

His Arg Ala His Leu His Gly Thr Arg Tyr Tyr Pro Ser Pro Glu Val

355 360 365

Phe Leu Tyr Phe Val Ser Gln Leu Cys Arg Phe Ser Lys Arg Glu Pro

370 375 380

Thr Leu Gln Leu Leu Glu Thr Leu Leu Thr Asp Arg Leu Lys Glu Arg

385 390 395 400

Ile Gln Val Lys Ala Asp Thr Leu Ser Leu Ala Met Arg Ile Leu Ala

405 410 415

Cys Leu Ser Val Gly Ile Ser Gln Val Glu Val Asp Val Arg Glu Leu

420 425 430

Leu Ala Leu Gln Cys Lys Asp Gly Ser Trp Glu Pro Gly Ser Phe Tyr

435 440 445

Arg Phe Gly Ser Ser Lys Met Asn Val Gly Asn Arg Gly Leu Thr Thr

450 455 460

Ala Leu Ala Thr Arg Ala Val Glu Leu Tyr Gln Gly Thr Arg Ile Arg

465 470 475 480

Ser Lys Gly Thr Glu

485

<210> 61

<211> 1458

<212> DNA

<213> 乌达加瓦曲霉(Aspergillus udagawae)_GAO87501.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 61

atgacccgac agaaatcgcc tcaatacaaa gcaatcatct ttgacctagg ggatgtcttt 60

ttcacctggg acgcccccaa agacactgct gtcttgccca acctcttcaa gaaaatgctt 120

acctcgccaa cctggtcaga ttacgagcgc ggcaagttga gcgaagaaag ctgctacgag 180

agactggccg aacagtttga cgttgactcg tcggaaatcg cgcgcagctt aaggaaagca 240

cagcagtctc ttaccacaga cgcagcaatc gtgagcctga tatcagagat cagagcgttg 300

gccggacata ttgccatcta cgccatgtcc aacatttccg ccccagctta tgcagctgtg 360

ctccagactc agcccgaaat gggcatcttt gacggagtgt tcccgtctgg atgctatggg 420

acgaggaagc cggagctgtt gttctataag aaagtcttgc aggagattgc agtgccgcca 480

aatcagatca tctttattga tgatcagcta gagaatgtag tttctgcgca gtcaacaggt 540

atgcacggca ttgtctacac cggtgcgggt gagctcagtc gacagctcag aaatctggtg 600

ttggaccctg tacaaagggg tcgagagttt ctacggcgca atgctggggc attgtatagt 660

atctgcgaga ctggtcaagt catccgggaa aacttctcgc agctgctcat cctagaggcg 720

acgggtgata gaagcctggt caaccttgaa tatcagcagc ggagctggaa tttctttcaa 780

ggaggtcccc cttctacgtc ggaaacattc ccagatgatg tcgacacaac atccattgcc 840

ttgatgattc tccctgccga tgataacaca gtcaactcgg ttctcggcga gatttccgag 900

gtagctaatg acgagggcat tgtaaatacg tactttgacc agacccgaca gcgaatcgac 960

ccagcagtct gcgtcaatgt cctccgtctc ttttatacct acggccgggg cgccactctc 1020

ccattgaccc tccagtgggt gtccgacgtt cttgagcatc gtgcgcactt acatggtacg 1080

cgatactacc ccagcccgga ggttttcctc tactttgtca gtcaactctg ccggttctcc 1140

aagagggaac cgacgctgca gctgctggag acgttgctca cggatcgcct caaggagcgc 1200

attcaggtca aggcagacac tctgtcactg gctatgcgga tcctggcatg cttgtctgtg 1260

ggtatatcac aagttgaagt ggatgtccga gagctgctcg ccttgcaatg caaggatgga 1320

tcgtgggaac ccggctcgtt ttaccggttt gggtcgtcca agatgaacgt tggtaatcga 1380

ggtcttacga ctgcgttggc gactagggcg gttgagttgt accaggggac tagaatacgc 1440

tctaagggca ccgagtag 1458

<210> 62

<211> 1458

<212> DNA

<213> 人工序列_GAO87501.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 62

atgactcgcc aaaaaagccc tcaatacaaa gcaattatct tcgatctggg tgacgttttc 60

ttcacctggg atgcgccgaa agatacggcc gtactgccga acctgttcaa gaaaatgctg 120

acctcgccga cctggagcga ctatgagcgt ggtaagctgt ctgaggaaag ctgttacgaa 180

cgcttggccg agcaatttga cgtggacagc agcgagatcg cgcgtagcct ccgtaaagcg 240

cagcaaagcc tgacgaccga cgcagccatc gtgagcctga tcagcgagat ccgcgcattg 300

gcgggtcaca ttgctatcta tgctatgtct aacatttctg cgccagcata cgcagcggtg 360

ttacagaccc agccggaaat gggtatcttt gatggtgttt ttccgagcgg ctgctatggt 420

acgcgtaaac cggaactgct gttttacaaa aaagtgcttc aagaaattgc ggttccgccg 480

aatcagatta tcttcattga cgatcagctg gaaaacgtcg tcagcgcaca gtccacgggc 540

atgcatggca ttgtttacac cggtgccggt gagctgagcc gtcaactgcg taatctggtc 600

ctggacccgg tgcagcgtgg tcgtgagttc ctgcgccgta atgctggcgc cctgtacagc 660

atttgtgaga ctggccaagt tatccgtgag aacttcagcc agctgctgat tctggaagca 720

accggcgatc gttcgctggt gaacctggag tatcaacaac gttcctggaa cttctttcag 780

ggtggccctc catccacgag cgaaactttt ccggatgatg ttgacacgac ctcaatcgcg 840

ctgatgattt taccggcgga cgataatacc gtcaatagcg tcctgggtga aatcagcgaa 900

gtcgcgaatg acgagggcat tgtgaatacc tatttcgatc agacccgcca acgtatcgat 960

ccggccgtgt gtgtcaacgt gttgcgcctg ttttacacct atggtcgtgg cgctacgctg 1020

ccgttgaccc tgcaatgggt tagcgacgtg ctggagcacc gtgcgcatct gcacggcacc 1080

cgctactatc cgtccccaga ggttttcctg tactttgtct ctcagctgtg ccgtttttcc 1140

aagcgcgaac cgaccctgca gctgctggaa acgctgttga ccgacagact gaaggaacgc 1200

atccaagtta aggcagatac gctgagcttg gcaatgcgta ttttggcgtg cctgagcgtg 1260

ggcatcagcc aggttgaggt tgacgtccgc gaactgctgg cgctgcagtg caaggacggt 1320

agctgggagc cgggtagctt ctaccgtttc ggtagcagca agatgaatgt cggtaaccgc 1380

ggtctgacga ccgctttggc gacccgtgcg gttgagctgt accagggtac gcgtattcgt 1440

agcaagggca ccgagtaa 1458

<210> 63

<211> 524

<212> PRT

<213> 云芝栓孔菌(Trametes versicolor)_XP_008034151.1蛋白

<400> 63

Met Ala Ser Pro His Arg Arg Tyr Thr Thr Leu Ile Leu Asp Leu Gly

1 5 10 15

Asp Val Leu Phe Ser Trp Ser Ser Lys Thr Asn Thr Pro Ile Pro Pro

20 25 30

Lys Lys Leu Lys Glu Ile Leu Ser Ser Leu Thr Trp Phe Glu Tyr Glu

35 40 45

Arg Gly Arg Ile Ser Gln Ala Glu Cys Tyr Asp Arg Val Ser Ser Glu

50 55 60

Phe Ser Leu Asp Ala Ala Thr Ile Ala Glu Ala Phe Gln Gln Ala Arg

65 70 75 80

Asp Ser Leu Arg Pro Asn Glu Glu Phe Leu Ala Leu Ile Arg Glu Leu

85 90 95

Arg Gln Gln Thr His Gly Gln Leu Thr Val Leu Ala Leu Ser Asn Ile

100 105 110

Ser Leu Pro Asp Tyr Glu Tyr Ile Met Ala Leu Asp Ser Asp Trp Thr

115 120 125

Ser Val Phe Asp Arg Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys

130 135 140

Pro His Leu Gly Ala Tyr Arg Arg Val Ile Ser Glu Met His Leu Asp

145 150 155 160

Pro Glu Thr Thr Val Phe Val Asp Asp Lys Leu Asp Asn Val Val Ser

165 170 175

Ala Arg Ser Leu Gly Met His Gly Val Val Phe Asp Ser Gln Glu Asn

180 185 190

Val Phe Gln Thr Leu Arg Asn Ile Phe Gly Asp Pro Ile His Arg Gly

195 200 205

Arg Asp Tyr Leu Arg Arg His Ala Gly Arg Leu Glu Thr Ser Thr Asp

210 215 220

Ala Gly Val Val Phe Glu Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu

225 230 235 240

Leu Thr Asn Asp Lys Ser Leu Ile Thr Thr Ser Asp Cys Pro Arg Thr

245 250 255

Trp Asn Phe Phe Arg Gly Lys Pro Leu Phe Ser Ala Ser Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Val Ala Leu Thr Val Leu Arg Pro Pro Arg

275 280 285

Thr Leu Val Asn Ser Ile Leu Asp Glu Met Leu Glu Tyr Val Asp Ala

290 295 300

Asp Gly Ile Met Gln Thr Tyr Phe Asp His Ser Arg Pro Arg Met Asp

305 310 315 320

Pro Phe Val Cys Val Asn Val Leu Ser Leu Phe Tyr Glu Tyr Gly Arg

325 330 335

Gly Gln Asp Leu Pro Lys Thr Leu Glu Trp Val Tyr Glu Val Leu Leu

340 345 350

His Arg Ala Tyr Ile Gly Gly Ser Arg Tyr Tyr Met Ser Ala Asp Cys

355 360 365

Phe Leu Phe Phe Met Ser Arg Leu Leu Gln Arg Ile Thr Asp Pro Ala

370 375 380

Val Leu Asn Arg Leu Arg Pro Leu Phe Val Glu Arg Met His Glu Arg

385 390 395 400

Val Ser Ala Pro Gly Asp Ser Met Glu Leu Ala Phe Arg Ile Leu Ala

405 410 415

Gly Ser Ser Val Gly Ile Gln Phe Pro Arg Asp Leu Glu Lys Leu Leu

420 425 430

Ala Ala Gln Cys Ala Asp Gly Gly Trp Asp Leu Cys Trp Phe Tyr Gln

435 440 445

Tyr Gly Ser Thr Gly Val Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala

450 455 460

Leu Ala Ile Lys Ala Ile Glu Ser Ala Ile Ala Arg Pro Pro Ser Pro

465 470 475 480

Ala Leu Ser Ala Val Ser Ser Ser Lys Leu Glu Val Pro Lys Pro Ile

485 490 495

Leu Gln Arg Pro Leu Ser Pro Arg Arg Leu Gly Asp Phe Leu Met Pro

500 505 510

Trp Arg Arg Ala Gln Arg Glu Val Ala Val Ser Ser

515 520

<210> 64

<211> 1575

<212> DNA

<213> 云芝栓孔菌(Trametes versicolor)_XP_008034151.1 - cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 64

atggcttcac ctcaccgcag gtatacgaca ctcatcctag acctgggcga cgtcctcttc 60

tcttggtcat ccaagaccaa cacacctatc cctcccaaga agctgaagga gatcctctcg 120

tccctgacct ggttcgagta cgagcgcggt cggatatcac aggccgagtg ctatgaccgg 180

gtcagctccg agttcagtct tgacgctgcc accatcgcag aagcgttcca gcaggctcgc 240

gactctctgc gaccgaacga agagttcctg gcgttgattc gcgaactccg ccaacaaacg 300

catggtcagc ttaccgtcct cgcgctctcg aacatctcac tccccgacta tgaatacatc 360

atggctctcg actcggactg gacgtcggtc ttcgaccgcg tcttcccttc tgccctcgtc 420

ggcgagcgca agccacatct gggggcgtac cgccgtgtca tctctgagat gcacctagac 480

ccagaaacga ccgtctttgt ggacgacaag ctggacaacg tggtgtccgc gcgatcgctc 540

gggatgcacg gcgtggtctt cgactcccag gagaacgtct tccagacgct gaggaatatc 600

ttcggcgacc cgatacatcg cggacgtgac tatctccgca ggcatgccgg tcgtctggag 660

acatctacgg acgccggcgt tgtcttcgag gaaaacttta cgcagctcat catctacgaa 720

ctaacaaatg acaaatccct catcacgaca tcagactgtc cccgcacttg gaacttcttc 780

cgcgggaagc ccttgttctc ggcctcgttt cccgacgatg tggacacgac gtcggttgcc 840

ctgacagtgt tgcgcccacc ccgcacgctt gtcaactcga tcttggacga gatgctagag 900

tatgtcgacg ccgacggcat catgcagacc tacttcgacc actcgcgccc gcggatggat 960

ccgttcgtct gtgtcaacgt cctgtcgctg ttctacgagt acggccgggg acaggacctc 1020

ccgaagaccc tcgaatgggt atacgaggtt ctgctgcacc gcgcctacat cggcggctcg 1080

cggtactaca tgtccgcgga ctgcttcctc ttcttcatga gccgccttct ccaacgtatc 1140

accgacccag ccgtcctgaa ccgcctccgc ccgttgttcg tcgagcgcat gcacgaacgt 1200

gtcagcgcac cgggcgactc catggagctc gcgttccgca tcctcgctgg ctcgtccgtc 1260

ggcatccagt tcccacgtga cctggagaag ctcctcgccg cgcagtgcgc cgacggcggc 1320

tgggacctgt gctggttcta ccagtatggg tccaccggcg tgaaggcagg caaccgcggg 1380

ctcaccaccg cgctcgccat caaggctatc gagagcgcta tcgcgcgccc tccgtccccc 1440

gctctatcag ctgtatcgtc gtcgaaactg gaagtgccga aaccaattct ccagcgtccc 1500

ctcagcccgc gccggcttgg cgacttcctg atgccctgga ggagagcaca gcgcgaggtc 1560

gcggtttcca gctag 1575

<210> 65

<211> 1575

<212> DNA

<213> 人工序列_XP_008034151 - 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 65

atggctagcc cgcaccgtcg ctatactact ctgattctgg atttgggtga tgttttgttt 60

agctggagca gcaaaaccaa tacgcctatt ccgccgaaaa agctgaaaga aatcctgtct 120

agcctgacct ggttcgagta cgagcgcggt cgcatttctc aagccgagtg ctatgaccgt 180

gtgagctctg agtttagcct ggacgcagcg accattgcag aggcattcca acaggctcgt 240

gactcgctgc gcccgaacga agaatttctg gcgttgattc gtgagctgcg ccagcagacc 300

cacggccaac tcaccgttct ggcactgagc aacatctccc tgccggatta cgagtacatc 360

atggctctgg atagcgattg gaccagcgtc tttgatagag ttttcccgag cgcgctggtt 420

ggtgagcgta agccgcatct gggtgcttac cgtcgtgtca ttagcgagat gcatctggac 480

ccggagacta cggtgtttgt ggacgacaaa ctggacaacg ttgtctccgc gcgcagcctg 540

ggtatgcacg gcgtcgtttt tgactcacaa gaaaatgttt tccagacgct gcgtaacatt 600

ttcggtgacc ctatccaccg tggccgcgac tatttgcgtc gtcatgccgg tcgtttggaa 660

accagcaccg acgcgggcgt tgtttttgaa gaaaacttca cccagctgat catctacgaa 720

ctgacgaatg acaagagcct gatcaccacg agcgattgtc cgcgcacctg gaacttcttc 780

cgtggtaagc cgctgtttag cgcgtccttc ccagacgatg tcgatacgac ttcggtggcc 840

ctgaccgttc tgcgcccacc gcgcaccctg gtaaacagca tcctggacga aatgttagaa 900

tacgtcgatg cggatggtat tatgcagacc tatttcgacc acagccgtcc gcgcatggac 960

ccgtttgtgt gtgtgaatgt gttgagcctg ttctatgagt acggccgtgg tcaagatctg 1020

ccaaaaaccc tggaatgggt ctacgaagtc cttctgcatc gtgcctacat cggtggctcc 1080

cgttattaca tgagcgcaga ttgctttttg ttctttatgt ctcgtctgct gcagcgcatc 1140

acggaccctg ccgtgctgaa tcgtctgcgt ccgctgttcg tggagcgtat gcacgagcgc 1200

gtgtctgccc cgggtgacag catggaactg gcgttccgta tcctggcggg cagcagcgtg 1260

ggtattcaat ttccgcgtga tttggagaaa ctgctggctg cgcagtgtgc ggacggtggc 1320

tgggatctgt gctggtttta tcaatacggt agcaccggcg ttaaggccgg caatcgtggc 1380

ctgacgacgg cactggcaat taaggccatt gagtccgcga ttgcgcgtcc gccgagcccg 1440

gcattgagcg cggtcagcag cagcaaactg gaagtgccga agccgatctt gcagcgtcca 1500

ctgagcccgc gtcgtctggg tgacttcctg atgccgtggc gccgtgcgca acgcgaagtc 1560

gcggttagct cctaa 1575

<210> 66

<211> 527

<212> PRT

<213> 污叉丝孔菌(Dichomitus squalens)_XP_007369631.1蛋白

<400> 66

Met Ala Ser Ile His Arg Arg Tyr Thr Thr Leu Ile Leu Asp Leu Gly

1 5 10 15

Asp Val Leu Phe Arg Trp Ser Pro Lys Thr Glu Thr Ala Ile Pro Pro

20 25 30

Gln Gln Leu Lys Asp Ile Leu Ser Ser Val Thr Trp Phe Glu Tyr Glu

35 40 45

Arg Gly Arg Leu Ser Gln Glu Ala Cys Tyr Glu Arg Cys Ala Glu Glu

50 55 60

Phe Lys Ile Glu Ala Ser Val Ile Ala Glu Ala Phe Lys Gln Ala Arg

65 70 75 80

Gly Ser Leu Arg Pro Asn Glu Glu Phe Ile Ala Leu Ile Arg Asp Leu

85 90 95

Arg Arg Glu Met His Gly Asp Leu Thr Val Leu Ala Leu Ser Asn Ile

100 105 110

Ser Leu Pro Asp Tyr Glu Tyr Ile Met Ser Leu Ser Ser Asp Trp Thr

115 120 125

Thr Val Phe Asp Arg Val Phe Pro Ser Ala Leu Val Gly Glu Arg Lys

130 135 140

Pro His Leu Gly Cys Tyr Arg Lys Val Ile Ser Glu Met Asn Leu Glu

145 150 155 160

Pro Gln Thr Thr Val Phe Val Asp Asp Lys Leu Asp Asn Val Ala Ser

165 170 175

Ala Arg Ser Leu Gly Met His Gly Ile Val Phe Asp Asn Gln Ala Asn

180 185 190

Val Phe Arg Gln Leu Arg Asn Ile Phe Gly Asp Pro Ile Arg Arg Gly

195 200 205

Gln Glu Tyr Leu Arg Gly His Ala Gly Lys Leu Glu Ser Ser Thr Asp

210 215 220

Asn Gly Leu Ile Phe Glu Glu Asn Phe Thr Gln Leu Ile Ile Tyr Glu

225 230 235 240

Leu Thr Gln Asp Arg Thr Leu Ile Ser Leu Ser Glu Cys Pro Arg Thr

245 250 255

Trp Asn Phe Phe Arg Gly Glu Pro Leu Phe Ser Glu Thr Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Val Ala Leu Thr Val Leu Gln Pro Asp Arg

275 280 285

Ala Leu Val Asn Ser Val Leu Asp Glu Met Leu Glu Tyr Val Asp Ala

290 295 300

Asp Gly Ile Met Gln Thr Tyr Phe Asp Arg Ser Arg Pro Arg Met Asp

305 310 315 320

Pro Phe Val Cys Val Asn Val Leu Ser Leu Phe Tyr Glu Asn Gly Arg

325 330 335

Gly His Glu Leu Pro Arg Thr Leu Asp Trp Val Tyr Glu Val Leu Leu

340 345 350

His Arg Ala Tyr His Gly Gly Ser Arg Tyr Tyr Leu Ser Pro Asp Cys

355 360 365

Phe Leu Phe Phe Met Ser Arg Leu Leu Lys Arg Ala Asp Asp Pro Ala

370 375 380

Val Gln Ala Arg Leu Arg Pro Leu Phe Val Glu Arg Val Asn Glu Arg

385 390 395 400

Val Gly Ala Ala Gly Asp Ser Met Asp Leu Ala Phe Arg Ile Leu Ala

405 410 415

Ala Ala Ser Val Gly Val Gln Cys Pro Arg Asp Leu Glu Arg Leu Thr

420 425 430

Ala Gly Gln Cys Asp Asp Gly Gly Trp Asp Leu Cys Trp Phe Tyr Val

435 440 445

Phe Gly Ser Thr Gly Val Lys Ala Gly Asn Arg Gly Leu Thr Thr Ala

450 455 460

Leu Ala Val Thr Ala Ile Gln Thr Ala Ile Gly Arg Pro Pro Ser Pro

465 470 475 480

Ser Pro Ser Ala Ala Ser Ser Ser Phe Arg Pro Ser Ser Pro Tyr Lys

485 490 495

Phe Leu Gly Ile Ser Arg Pro Ala Ser Pro Ile Arg Phe Gly Asp Leu

500 505 510

Leu Arg Pro Trp Arg Lys Met Ser Arg Ser Asn Leu Lys Ser Gln

515 520 525

<210> 67

<211> 1584

<212> DNA

<213> 污叉丝孔菌(Dichomitus squalens)_XP_007369631.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 67

atggcctcaa tccaccgtcg atacactact ctcatcctcg acctcggcga cgtactcttt 60

cgttggtctc caaagactga gaccgccatt ccacctcaac aactcaagga tatcctctcc 120

tctgtcacct ggtttgagta cgaacgcggc agactatccc aggaagcatg ctacgagcgc 180

tgcgccgagg agttcaagat agaggcctcg gtcattgcag aagcctttaa gcaggctcgc 240

gggtcactgc ggcccaacga ggagttcatc gccttgatcc gtgacctccg ccgtgagatg 300

cacggtgacc ttaccgttct tgccctctcc aacatctccc tccccgacta cgaatacatc 360

atgtcgctaa gctcagattg gacgaccgtc ttcgatcgcg tattcccctc tgcactcgtt 420

ggcgagcgca agcctcatct gggatgctat cgcaaggtca tctcggagat gaacctagaa 480

cctcagacga ctgtgttcgt ggatgacaag cttgacaacg tcgcgtctgc tcgctcactt 540

ggtatgcacg gcatcgtgtt tgacaaccaa gccaacgtct tccgccaact ccgcaatatc 600

ttcggagacc ccatccgccg tggccaagag tatctccgtg ggcatgctgg caaactcgag 660

tcttcgaccg acaacgggtt gatcttcgag gagaacttca cacagctgat catctacgag 720

ttgacgcaag acaggactct catctcgctt tcagaatgtc ctcgtacttg gaatttcttc 780

cgaggcgaac cgctattctc ggagaccttc ccggatgatg tcgacacaac atctgtggcg 840

ttgacggtat tgcaaccgga cagagcactg gtcaactccg ttctagacga gatgctggag 900

tatgtcgacg ccgatggcat catgcagaca tacttcgatc gttcacgacc acgcatggac 960

cccttcgtct gcgtgaacgt actctccctg ttctacgaga acggtcgtgg tcacgagctc 1020

cctcgcacat tggactgggt ctacgaggtg ctcctccatc gcgcgtacca cggcggttcg 1080

cgttattacc tgtcgcccga ctgctttcta ttcttcatga gccgcctact caagcgcgca 1140

gacgatccag cagtccaggc tcggctccgc ccgctcttcg tcgagcgggt gaacgagcga 1200

gtaggcgccg ctggcgactc gatggacctc gccttccgca tcctcgccgc agcgtctgtt 1260

ggcgtccagt gcccccgcga tctggaaagg ttgactgccg ggcaatgcga cgacggtgga 1320

tgggacctct gctggttcta cgtgttcggc tcgacgggcg tgaaggcggg caaccgcggc 1380

ctcacaacgg ccctcgctgt cacggccata cagacggcca tcggacgccc cccttcgccc 1440

agtccctccg cggcctcctc gtctttcaga cctagttccc cttacaaatt cctaggcatt 1500

tcgcgcccag ctagccccat tcgctttggc gacttacttc gcccatggcg gaagatgagc 1560

aggtcgaact tgaagtctca atga 1584

<210> 68

<211> 1584

<212> DNA

<213> 人工序列_XP_007369631.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 68

atggcaagca ttcatcgtcg ctatactacg ctgattctgg acctgggtga tgttttgttc 60

cgctggagcc cgaaaaccga gactgcgatt cctccgcaac aactgaaaga catcctgagc 120

agcgtcacct ggttcgagta cgagcgtggc cgtctgagcc aagaggcttg ctacgagcgt 180

tgcgccgaag agttcaagat tgaagccagc gtgattgcgg aagcgttcaa acaagcgcgt 240

ggtagcctgc gtccgaacga agaatttatc gcactgatcc gtgatctgcg tcgcgagatg 300

catggtgacc tgaccgttct ggctctgagc aatatctcgt tgccggatta cgagtatatt 360

atgtctctga gcagcgactg gacgacggtc tttgatcgtg tgttcccgtc agctctggtg 420

ggcgagcgta aaccgcactt gggttgctat cgcaaggtca tcagcgagat gaacctggaa 480

cctcagacca cggtctttgt ggacgataaa ctggataatg tcgcaagcgc gcgtagcctg 540

ggtatgcacg gtatcgtgtt tgataatcaa gcgaatgtgt ttcgccagct gcgtaatatt 600

ttcggtgatc caatccgtcg cggtcaagag tatctgcgtg gccatgccgg taaattggag 660

agcagcacgg acaatggttt gatctttgaa gagaacttca cccagctgat catttatgaa 720

ctgacccagg accgcacgtt gatcagcctg tcggagtgtc cgcgtacctg gaacttcttc 780

cgtggcgagc cgttgttttc tgaaaccttc ccggacgacg tggacaccac gtccgttgca 840

ctgacggttc tgcaaccgga tcgcgcactg gttaacagcg tgctggacga aatgctggaa 900

tatgtcgatg cggatggcat catgcagacg tatttcgacc gctcgcgtcc gcgtatggac 960

ccgtttgttt gcgtcaacgt actgagcctg ttttacgaga acggtcgtgg tcacgaactg 1020

ccgcgcactc tggattgggt gtacgaagtc ctgctccacc gcgcctacca cggtggttcc 1080

cgttactacc tgagcccgga ctgtttcttg ttttttatga gccgtctgct gaaacgtgca 1140

gacgacccag cggttcaggc gagattgcgt ccgctgtttg tggaacgcgt taacgaacgt 1200

gttggcgcgg ccggtgatag catggacctg gcgtttcgca ttctggccgc agcgagcgtg 1260

ggtgtgcagt gtccgcgcga cctggagcgt ctgaccgctg gtcaatgcga tgatggcggc 1320

tgggatctgt gttggttcta cgttttcggc agcaccggcg ttaaggccgg taatcgtggt 1380

ctgaccacgg cgctggcagt caccgcgatc cagaccgcca tcggccgtcc gcctagcccg 1440

agcccgtccg cggcaagctc cagcttccgc ccgagcagcc cgtacaagtt tctgggtatt 1500

agccgtccgg cgtccccaat tcgcttcggt gaccttctgc gtccgtggcg taaaatgtct 1560

cgctctaacc tgaagtccca gtaa 1584

<210> 69

<211> 495

<212> PRT

<213> 牛樟芝(Antrodia cinnamomea)_ACg006372蛋白

<400> 69

Met Arg Arg Asn Val Leu Asn Lys Ala Thr His Ser Gln Ser Pro Leu

1 5 10 15

Lys Pro Asn Ile Thr Thr Leu Ile Phe Asp Leu Gly Asp Val Leu Leu

20 25 30

Thr Trp Ser Asp Ser Thr Pro Lys Ser Pro Leu Pro Pro Lys Ile Val

35 40 45

Lys Gly Ile Leu Arg Ser Leu Thr Trp Phe Glu Tyr Glu Lys Gly Asn

50 55 60

Leu Thr Glu Ser Gln Thr Tyr Gly Gln Val Ala Gln Glu Phe Gly Val

65 70 75 80

Asp Ala Ser Glu Val Lys Ala Ser Phe Glu Ala Ala Arg Asp Ser Leu

85 90 95

Lys Ser Asn Pro Met Leu Leu Gln Leu Ile Arg Ser Leu Lys Asp Ser

100 105 110

Gly His Val Ile Tyr Ala Met Ser Asn Ile Ser Ala Pro Asp Trp Glu

115 120 125

Phe Leu Lys Thr Arg Ala Asp Leu Ser Asp Trp Ala Leu Phe Asp Arg

130 135 140

Val Phe Pro Ser Ala Glu Ala His Asp Arg Lys Pro Asn Ile Gly Phe

145 150 155 160

Tyr Gln His Val Ile Asn Glu Thr Gly Leu Asn Pro Ser Asn Thr Val

165 170 175

Phe Val Asp Asp Arg Ile Glu Asn Val Val Ser Ala Arg Ser Ala Gly

180 185 190

Met His Gly Ile Val Phe Asp Asp Ile Asn Asn Val Ile Arg Gln Leu

195 200 205

Lys Asn Leu Cys Glu Asp Pro Ile His Arg Ala Arg Ser Phe Leu Tyr

210 215 220

Ala Asn Lys Lys Cys Leu Asn Thr Val Ser Thr Asp Gly Thr Ile Val

225 230 235 240

Ser Glu Asn Phe Ser Gln Leu Leu Ile Leu Glu Ala Ile Gly Asp Glu

245 250 255

Ser Leu Val Asp Phe Val Arg His Glu Gly Arg Phe Asn Phe Phe Gln

260 265 270

Gly Glu Ala Lys Leu Ile Met Thr Asn His Tyr Pro Asp Asp Phe Asp

275 280 285

Thr Thr Ser Ile Gly Leu Thr Val Val Pro Tyr Ile Asp Asp Lys Thr

290 295 300

Arg Asn Arg Val Met Asp Glu Ile Leu Ala Tyr Gln Ser Glu Asp Gly

305 310 315 320

Ile Val Leu Val Tyr Phe Asp His Lys Arg Pro Arg Ile Asp Pro Val

325 330 335

Val Cys Val Asn Val Leu Thr Leu Phe Tyr Arg Tyr Gly Arg Gly His

340 345 350

Gln Leu Gln Lys Thr Leu Asp Trp Val Glu Gln Val Leu Ile Asn Arg

355 360 365

Ala Cys Ala Ser Gly Thr Phe Tyr Tyr Ala Thr Glu Glu Gln Phe Leu

370 375 380

Phe Phe Leu Ser Arg Leu Ile Gln Ser Ser Pro Asp Val Arg Gln Arg

385 390 395 400

Leu Glu Gly Val Phe Lys Arg Arg Val Val Glu Arg Phe Gly Ala Asp

405 410 415

Gly Asp Ala Leu Ala Met Ala Met Arg Ile His Thr Ala Ala Ser Val

420 425 430

Gly Leu Val Asp His Val Asp Leu Asp Lys Leu Phe Ala Leu Gln Gln

435 440 445

Asn Asp Gly Ser Trp Arg Asp Ser Ala Phe Tyr Arg Phe Pro Ser Ala

450 455 460

Arg Gln Leu Ala Ser Asn Asp Gly Leu Thr Thr Ala Ile Ala Ile Gln

465 470 475 480

Ala Ile Gln Ala Ala Glu Arg Leu Arg Glu Asp Gly Asn Val Leu

485 490 495

<210> 70

<211> 1488

<212> DNA

<213> 牛樟芝(Antrodia cinnamomea)_ACg006372 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 70

atgaggcgaa acgtactcaa caaagcaaca cattctcagt caccattgaa gcccaacatc 60

acgacgctca tatttgactt gggcgacgta cttctcacgt ggtccgactc aacacctaaa 120

tctccactgc ccccaaaaat tgtcaaggga atactacgtt cactgacctg gtttgagtac 180

gagaaaggga acttgacaga gtcccagacc tacgggcaag ttgctcagga atttggagtg 240

gatgcttccg aagtcaaagc ttccttcgaa gcagctcgcg actcgctcaa gagcaaccca 300

atgcttctcc agttgatccg tagcctcaaa gactctggcc acgtcattta cgcaatgtct 360

aacatatctg ctcccgactg ggaatttttg aagacgcggg cagacctctc agattgggct 420

ctttttgaca gagtcttccc ttctgccgaa gcgcatgacc gcaagccgaa cattggtttc 480

tatcagcacg tcataaacga gactggtctg aacccgtcca acactgtctt tgtcgatgac 540

aggatcgaga atgttgtatc cgcacgctca gcaggaatgc acgggatcgt gtttgacgac 600

ataaataatg tgatccgaca gttgaaaaac ctctgcgagg atccgattca ccgcgcacga 660

tcttttcttt atgcaaataa gaagtgtttg aatacggtta gcacagatgg cacaattgtg 720

agcgagaact tctcgcaatt gttgatcctt gaggccattg gcgacgaaag cctagtcgac 780

tttgtgaggc atgagggccg attcaacttc ttccaggggg aggccaaact catcatgacg 840

aatcactacc ccgatgattt cgatactaca tccataggtt taaccgttgt tccatatatt 900

gacgacaaga ctagaaatag agttatggat gagatcctgg cctaccaaag cgaagacggc 960

attgtgctgg tatactttga ccacaagcgc cccaggattg atcctgttgt ctgtgtcaat 1020

gtcctcaccc tcttctatag gtatggccgt gggcaccagc ttcaaaagac actggattgg 1080

gtcgaacagg tcctgatcaa ccgtgcgtgt gcgtccggca cgttctatta cgcaacagag 1140

gaacaattcc tctttttcct ctcccgcctg atccaaagct ctccggacgt acgacagcgg 1200

ttggaagggg tctttaaaag aagagtagtc gagcggtttg gtgcagacgg cgacgctctc 1260

gctatggcga tgcgcattca caccgcggcg agcgtgggcc tcgttgacca tgtcgatctt 1320

gacaagctgt tcgcattgca gcaaaatgac ggttcttgga gagacagcgc tttctacaga 1380

tttccgtcgg ccaggcaact ggctagtaac gacggcttga cgactgcaat cgctattcag 1440

gccattcaag ctgcggagag gctcagggag gatgggaacg tgctttga 1488

<210> 71

<211> 1488

<212> DNA

<213> 人工序列_ACg006372 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 71

atgcgccgta atgtcctgaa caaagcaacc catagccagt caccgttgaa accgaatatc 60

accacgctga tttttgactt gggcgatgtc ctgctgacct ggagcgacag cactccgaaa 120

tctccgttgc cgccgaagat cgtcaagggc atcctgcgta gcctgacttg gttcgagtac 180

gaaaagggca atttgaccga aagccaaacg tatggtcagg tcgcgcaaga atttggtgtg 240

gatgcctctg aagtgaaggc cagctttgag gctgcgcgtg atagcttgaa atcgaatccg 300

atgctgctgc agctgattcg cagcctgaaa gattccggtc acgtgatcta cgccatgagc 360

aacatcagcg cgcctgattg ggaatttctg aaaacccgcg ctgacctgtc tgactgggcc 420

ctgtttgacc gcgtgttccc gtctgccgag gcacatgacc gcaaaccgaa cattggcttt 480

taccaacacg tgatcaatga aacgggtctg aatccatcca ataccgtgtt cgttgacgac 540

cgtattgaaa acgttgttag cgcacgtagc gctggtatgc acggtatcgt tttcgatgac 600

attaacaacg tcattcgcca gctgaagaat ctgtgcgagg acccaattca ccgtgcacgt 660

tcctttttgt atgcgaacaa aaagtgcctg aataccgtga gcaccgatgg tacgatcgtc 720

agcgagaact ttagccagct tctgattctg gaagccattg gtgacgagtc cctggtagac 780

ttcgtccgcc atgagggccg ttttaacttc ttccagggtg aggcaaagct gatcatgacc 840

aatcactacc cggacgattt cgataccacg agcattggtc tgaccgttgt cccgtatatc 900

gatgacaaaa cgcgtaatcg tgtgatggat gaaatcctgg cgtatcagtc cgaggatggt 960

atcgttctgg tgtacttcga tcacaagcgt ccgcgcattg acccggtcgt ttgtgtgaac 1020

gttctgacgc tgttctaccg ctatggtcgt ggccatcaac tgcagaaaac cctggactgg 1080

gttgagcaag tcctgattaa tcgtgcgtgt gcgagcggca cgttctacta cgcgaccgaa 1140

gaacagttcc tgtttttcct gagccgtctg attcagtcga gccctgacgt gcgccaacgt 1200

ctggaaggcg tgttcaagcg tcgtgtcgtt gagcgctttg gtgcggacgg tgatgccctg 1260

gcaatggcga tgcgtatcca taccgcagcg agcgttggcc tggtggacca cgtggatctg 1320

gataagctgt tcgcgctgca acagaacgac ggtagctggc gcgatagcgc gttttatcgt 1380

tttccgagcg cgcgtcaact cgcgagcaac gacggcttga ccacggcaat tgctattcag 1440

gccatccaag cggctgagag attacgtgag gatggtaacg ttctgtaa 1488

<210> 72

<211> 543

<212> PRT

<213> 焦曲霉(Aspergillus ustus)_KIA75676.1蛋白

<400> 72

Met Val Arg Ala Leu Ile Leu Asp Leu Gly Asp Val Leu Phe Asn Trp

1 5 10 15

Asp Ala Pro Lys Ser Thr Pro Val Ser Arg Lys Thr Leu Ser Gln Met

20 25 30

Leu His Ser Asp Ile Trp Gly Glu Tyr Glu Cys Gly Gln Leu Thr Glu

35 40 45

Pro Glu Ser Tyr Lys Ala Leu Ala Ser Arg Tyr Ser Cys Gln Ala Gln

50 55 60

Asp Val Ala Asp Thr Phe Tyr Leu Ala Arg Glu Ser Leu Arg Leu Asp

65 70 75 80

Ala Thr Phe Lys Thr Phe Leu Gln Asp Leu Lys Gln Arg Ala Asn Gly

85 90 95

Ser Leu Arg Val Tyr Gly Met Ser Asn Ile Ser Gln Pro Asp Tyr Glu

100 105 110

Val Leu Leu Ser Lys Ala Asp Asp Leu Ser Leu Phe Asp Lys Ile Phe

115 120 125

Pro Ser Gly His Val Gly Met Arg Lys Pro Asp Leu Ala Phe Phe Arg

130 135 140

His Val Leu Arg Glu Ile Ser Thr Ala Ser Glu Asp Ile Val Phe Val

145 150 155 160

Asp Asp Asn Leu Glu Asn Val Thr Ser Ala Arg Ser Leu Gly Met Gln

165 170 175

Gly Ile Val Phe Arg Asp Lys Glu Asp Val Gln Arg Gln Leu Arg Asn

180 185 190

Leu Phe Gly Ser Pro Ala Glu Arg Gly Arg Glu Tyr Leu Ser Ile Asn

195 200 205

Lys Thr Lys Leu Gln Ser Val Thr Thr Thr Asn Ile Pro Ile Leu Asp

210 215 220

Asn Phe Gly Gln Leu Leu Ile Leu Glu Ala Thr Arg Asp Pro Asp Leu

225 230 235 240

Val Ser Met His Pro Gly Gln Arg Thr Trp Asn Phe Phe Ile Gly Ser

245 250 255

Pro Thr Leu Thr Thr Asp Ala Phe Pro Asp Asp Met Asp Thr Thr Ser

260 265 270

Leu Gly Leu Ser Ile Ile Pro Pro Ser Pro Glu Ile Ala Ala Ser Val

275 280 285

Met Asp Glu Ile Val Thr Arg Leu Asn Lys Asp Gly Ile Val Pro Thr

290 295 300

Tyr Phe Asp Ser Thr Arg Pro Arg Val Asp Pro Ile Val Cys Val Asn

305 310 315 320

Val Leu Thr Leu Phe Ala Lys Tyr Gly Arg Glu Asp Glu Leu Ser Gly

325 330 335

Thr Ile Ala Trp Val Arg Asp Val Leu Tyr His Arg Ala Tyr Leu Ala

340 345 350

Gly Thr Arg Tyr Tyr Ala Ser Pro Glu Ala Phe Leu Phe Phe Phe Thr

355 360 365

Arg Phe Thr Arg Asn Leu Arg Pro Gly Pro Arg Lys Gln Glu Leu Thr

370 375 380

Ala Leu Leu Ser Gln Arg Leu Gln Glu Arg Asn Lys Thr Pro Val Asp

385 390 395 400

Ala Leu Ala Leu Ser Met Arg Ile Ile Ala Cys Leu Thr Leu Gly Ile

405 410 415

Glu Ser Pro Ala Asp Asp Val Ala Thr Leu Thr Gly Met Gln Cys Gly

420 425 430

Asp Gly Gly Trp Pro Ala Cys Val Ile Tyr Lys Tyr Gly Ala Gly Gly

435 440 445

Leu Gly Ile Thr Asn Arg Gly Val Ser Thr Ala Phe Ala Val Lys Ala

450 455 460

Ile Thr Thr Thr Pro Leu Ala Val Gln Pro Glu Val Ser Val Ser Ala

465 470 475 480

Gly Ala Gly Gly Ser Ser Arg Pro Val Gly Ala Asp Ala Ala Ala Val

485 490 495

Ser Leu Arg Pro Arg Trp Arg Ala Val Val Gln Ser Leu His Pro Leu

500 505 510

Ser Arg Val Gly Gly Leu Val Ala Val Ile Phe Ala Ala Leu His Phe

515 520 525

Asn Leu Ala Trp Leu Tyr Asn Val Ser Leu Ala Ser Arg Ile Val

530 535 540

<210> 73

<211> 1632

<212> DNA

<213> 焦曲霉(Aspergillus ustus)_KIA75676.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 73

atggtccgcg cactgattct cgatctcggc gacgtcctct tcaactggga cgccccaaag 60

tcaacccccg tttcccgcaa gacactcagc cagatgctgc atagcgacat ctggggcgaa 120

tacgaatgtg gccaactgac agagccggaa agctacaagg cgcttgccag ccgctattct 180

tgccaggctc aagatgttgc agataccttc tatctagccc gcgaatcgct gaggctcgat 240

gcgaccttca agaccttcct gcaggacttg aagcagaggg ccaacggctc acttcgcgta 300

tatgggatgt ccaacatctc ccagcccgat tatgaggtcc tgctgtccaa ggcggatgac 360

ttgagcctgt ttgacaagat cttcccatcc ggccacgtcg ggatgcgtaa gcctgacctt 420

gcgttttttc gacatgtcct gcgtgagatc tcgacggcca gcgaggatat tgtgtttgtt 480

gacgacaacc tggagaacgt gacatctgcc cggtctctgg gcatgcaggg gattgtcttt 540

cgcgacaagg aggatgtaca gagacagctg cggaacctct ttggcagtcc tgctgaacgt 600

ggaagggagt atttgtccat caacaagaca aagctccaga gcgtcacgac gaccaatatc 660

cccattctcg acaactttgg ccagctcctt atcctcgaag ccaccagaga cccagacctg 720

gtgtccatgc atcctggaca gaggacctgg aactttttca tcggatctcc aactctgaca 780

acggacgcct tcccagacga tatggacacc acctcacttg gcctttctat tataccccca 840

agtcccgaga ttgcagcgtc cgtgatggat gagattgtga cccgcctgaa caaggacggc 900

attgtcccaa catattttga cagcaccaga ccccgcgtcg acccgatcgt ctgcgtcaac 960

gttctcaccc tcttcgctaa atacggccgc gaagacgagc tgtccgggac catagcctgg 1020

gtgcgcgatg tgctgtatca cagggcctac cttgcaggga ccagatacta cgcatcccca 1080

gaagcattcc ttttcttctt cacgcgcttc acccgaaacc tgcgcccggg cccgcgcaag 1140

caggagctca cggcgctgct gtcccagcgc ctgcaggagc gcaacaagac gcccgttgac 1200

gcacttgcgc tctcgatgcg gattattgcg tgcctcacgc tgggtattga atcccccgct 1260

gacgacgtgg ctaccctcac gggcatgcag tgtggggatg gcgggtggcc ggcctgtgtc 1320

atctacaagt acggcgccgg tgggctgggg atcacgaaca ggggggtctc gaccgcgttt 1380

gctgtcaagg caatcactac tactcctttg gcggtgcagc ctgaagttag tgtcagcgca 1440

ggtgcaggag gcagcagtcg ccctgtgggt gccgatgctg ctgcagtctc gctccgcccg 1500

agatggcgag ctgttgtgca gagtctccat ccgctctctc gggttggtgg gttggtggcc 1560

gtcatttttg ctgcactgca tttcaacttg gcctggcttt ataatgtgtc ccttgctagt 1620

aggatcgttt ag 1632

<210> 74

<211> 1632

<212> DNA

<213> 人工序列_KIA75676.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 74

atggttcgtg cattgatttt ggatttgggt gatgtgttgt ttaactggga tgcgcctaag 60

agcaccccgg tttcccgcaa gactctgagc caaatgctgc actcggatat ttggggcgag 120

tacgagtgtg gtcaactgac tgagccggag tcctataaag ccctggcgag ccgctatagc 180

tgccaggcgc aagatgtcgc tgacaccttt tacctggcgc gtgagagcct gcgtctggac 240

gcaacgttta agaccttcct gcaagatctg aagcaacgcg ccaacggttc tctgcgtgtc 300

tatggtatga gcaatatcag ccagccggat tacgaagtcc tgctgagcaa agctgacgat 360

ctcagcctgt ttgacaaaat ctttccgtcg ggtcacgttg gtatgagaaa gcctgacctg 420

gcgtttttcc gtcacgttct gcgtgagatc agcacggcta gcgaagatat tgtgtttgtt 480

gacgacaatt tggaaaacgt cacgtctgca cgctccctgg gtatgcaagg catcgtcttt 540

cgtgataagg aagatgtcca gcgccagctg cgcaatctgt tcggttcccc ggcagagcgc 600

ggtcgtgagt atctgagcat taataagacc aaactgcaga gcgtgaccac caccaatatc 660

ccgattctgg acaacttcgg tcagttgctg atcctggaag ctacccgtga cccggattta 720

gtcagcatgc atccaggcca acgtacgtgg aacttcttca ttggcagccc gaccttgacg 780

accgacgcgt ttccggacga tatggacacg acttctctgg gcctgagcat catcccgccg 840

agcccggaaa ttgcagcaag cgttatggac gaaatcgtca cccgtctgaa taaagatggt 900

attgtgccga cctacttcga cagcacgcgt ccacgtgtgg acccgatcgt ctgcgttaac 960

gtcctgacct tgtttgcgaa atatggtcgt gaagatgaac tgagcggcac gattgcgtgg 1020

gtccgcgacg ttctgtatca tcgcgcatac ctggcgggca cgcgctacta cgcgtcccca 1080

gaggccttcc tgttcttctt tacgcgtttc acccgcaatc tgcgtccggg tccgcgtaaa 1140

caagaactta cggcgctgct gagccagcgt ctgcaggaac gcaacaagac gccggttgac 1200

gctctggccc tgagcatgcg tatcatcgcc tgtctgaccc tgggcattga gagcccggca 1260

gacgacgtgg ccaccctgac cggtatgcag tgtggtgatg gtggctggcc ggcgtgcgtg 1320

atctacaaat atggtgcggg tggcttgggt atcacgaatc gtggcgttag cactgccttc 1380

gcggtgaaag cgattacgac caccccgctg gcagtgcagc cagaagtcag cgtcagcgct 1440

ggtgccggcg gctccagccg cccggttggt gcggatgcgg cagcggttag cttgcgtccg 1500

cgttggcgtg cggttgtgca gagcctgcat ccgctgagcc gcgtgggtgg cctggttgcc 1560

gtgatcttcg cggcactgca ctttaacctg gcgtggctgt acaacgtaag cctggctagc 1620

cgtattgtgt aa 1632

<210> 75

<211> 477

<212> PRT

<213> 米曲霉(Aspergillus oryzae)_XP_001820867.2蛋白

<400> 75

Met Thr Arg Trp Lys Ser Ser Gln Tyr Gln Ala Ile Ile Phe Asp Leu

1 5 10 15

Gly Gly Val Ile Leu Thr Trp Asp Leu Pro Glu Asp Thr Val Ile Ser

20 25 30

Ala Gln Ile Phe Lys Arg Met Leu Thr Ser Gln Thr Trp Ser Asp Tyr

35 40 45

Glu Arg Gly Asn Leu Ser Glu Asn Gly Cys Tyr Gln Arg Leu Ala Glu

50 55 60

Asp Phe Gly Ile Asp Ser Ala Asp Ile Ala His Thr Val Arg Gln Ala

65 70 75 80

Arg Glu Ser Leu Val Thr Asp Thr Ala Ile Met Asn Ile Ile Ser Glu

85 90 95

Ile Arg Ala Gly Ala Asn His Ile Ala Ile Phe Ala Met Ser Asn Ile

100 105 110

Ser Gln Pro Asp Tyr Ala Ala Leu Leu Leu Asp His Arg Gly Met Cys

115 120 125

Ser Phe Asp Arg Val Phe Pro Ser Gly Cys Tyr Gly Thr Arg Lys Pro

130 135 140

Glu Leu Ser Phe Tyr Asn Lys Val Leu Arg Glu Ile Asp Thr Pro Pro

145 150 155 160

Glu Asn Val Ile Phe Val Asp Asp Gln Leu Glu Asn Val Ile Ser Ala

165 170 175

Gln Ser Ile Gly Ile His Gly Ile Ala Tyr Thr Asn Ala Ala Glu Leu

180 185 190

Gly Arg Gln Leu Arg Asn Leu Ile Phe Asp Pro Val Glu Arg Gly Arg

195 200 205

Glu Phe Leu Arg Arg Asn Ala Gly Glu Phe His Ser Ile Thr Glu Thr

210 215 220

Asp Gln Ile Val Arg Glu Asn Phe Ser Gln Leu Leu Ile Leu Glu Ala

225 230 235 240

Thr Gly Asp Lys Ser Leu Val Ser Leu Glu Tyr His Gln Lys Ser Trp

245 250 255

Asn Phe Phe Gln Gly Asn Pro Ile Leu Thr Thr Glu Thr Phe Pro Asp

260 265 270

Asp Val Asp Thr Thr Ser Leu Ala Leu Met Thr Leu Pro Thr Asp Thr

275 280 285

Lys Thr Ala Asn Leu Leu Leu Asp Gln Ile Leu Gly Leu Val Asn Ala

290 295 300

Asp Glu Ile Val Thr Thr Tyr Phe Asp Gln Thr Arg Glu Arg Ile Asp

305 310 315 320

Pro Val Val Cys Val Asn Val Leu Arg Leu Phe Cys Thr Tyr Gly Arg

325 330 335

Gly Ile Ala Leu Pro Leu Thr Leu Gln Trp Val Tyr Asp Val Leu Ala

340 345 350

His Arg Ala Tyr Ile Asn Gly Thr Arg Tyr Tyr Thr Ser Pro Glu Ser

355 360 365

Phe Leu Tyr Phe Val Gly Gln Leu Cys Arg Phe Ser Thr Gly Val Leu

370 375 380

Ala Leu Arg Pro Leu Glu Thr Leu Leu Ile Asp Arg Leu Lys Glu Arg

385 390 395 400

Leu Gln Val Lys Ala Asp Pro Leu Ser Leu Ala Met Arg Ile Leu Thr

405 410 415

Cys Leu Ser Val Gly Val Ser Gln Val Glu Val Asp Leu Arg Glu Leu

420 425 430

Leu Ser Met Gln Cys Glu Asp Gly Ser Trp Glu His Cys Pro Phe Thr

435 440 445

Arg Tyr Gly Leu Ser Lys Val Ser Ile Gly Asn Arg Gly Leu Thr Thr

450 455 460

Ala Phe Val Val Lys Ala Val Glu Met Cys Arg Gly Ser

465 470 475

<210> 76

<211> 1434

<212> DNA

<213> 米曲霉(Aspergillus oryzae)_XP_001820867.2 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 76

atgactcgat ggaaatcgtc ccaataccaa gcaattatct ttgacctagg cggtgtcatt 60

ttaacatggg acctcccgga agacactgtg atatcggccc agatctttaa gagaatgctc 120

acatcgcaga catggtcaga ttatgagcgc ggaaatctca gcgaaaatgg ttgctaccag 180

aggttggccg aggattttgg cattgactct gccgacattg cacataccgt tagacaagca 240

cgggaatccc ttgtcactga taccgctatc atgaacatta tatctgagat cagagctggg 300

gctaaccata ttgctatctt cgctatgtcg aacatctccc aaccagatta tgcggctctg 360

ctccttgatc atcgcgggat gtgcagtttt gaccgggtgt tcccatctgg atgctacggg 420

acaaggaaac cagagctctc attctataac aaagtcttgc gggagattga cacgccaccg 480

gaaaacgtca tctttgtcga tgatcagctg gaaaatgtga tctctgcgca gtccattggc 540

atacacggga ttgcctatac gaatgctgct gaactcggtc gacagcttag gaacctaata 600

tttgaccctg tagagagggg tagggaattc ttacggcgca atgctggaga gttccatagc 660

atcactgaaa ccgatcaaat tgttcgggaa aatttctcac agttgctcat tctagaagcg 720

actggtgata agagtctggt atctcttgaa tatcaccaga agagctggaa tttcttccaa 780

ggaaacccta ttctcacgac agagacattc ccagatgatg ttgacacaac atctcttgcc 840

ttgatgactc tacctacaga cacaaaaact gcaaatttgt tactcgacca gattttgggg 900

ctagtcaacg ctgatgaaat cgtaacaaca tactttgacc agacccgaga acggatcgat 960

ccagtagtct gcgtcaatgt ccttcgtctc ttttgcacct acggccgggg cattgcgctc 1020

cctttgactc ttcagtgggt gtacgacgtc ctcgctcatc gggcatatat aaacggtaca 1080

cgttactaca caagtcccga aagcttccta tacttcgtcg gtcaactttg tcgattctca 1140

acaggggtac tggcacttcg gccgctggaa acgttgctta tagatcgtct caaggaacgt 1200

cttcaggtca aagcagatcc tctatcactc gctatgcgga tcttgacctg tttgtccgtt 1260

ggtgtgtctc aagttgaagt cgatctccga gagttgctct cgatgcagtg tgaagatggc 1320

tcgtgggaac attgtccatt cacccggtat ggtttgtcca aagtgagcat tggcaatcgg 1380

ggccttacaa ctgcttttgt ggtcaaggcg gttgaaatgt gtcgaggcag ttag 1434

<210> 77

<211> 1434

<212> DNA

<213> 人工序列_XP_001820867.2 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 77

atgactcgtt ggaaaagctc tcaatatcag gcaatcattt tcgatctggg cggtgttatt 60

ctgacctggg acttgccgga agatacggtt atctccgcgc aaatctttaa gcgtatgctg 120

accagccaga cctggtccga ttatgagcgc ggtaatctga gcgagaacgg ctgctatcaa 180

cgtttggcgg aagatttcgg catcgatagc gccgatattg cccacaccgt ccgtcaggca 240

cgtgagtccc tggtgaccga caccgccatc atgaatatca tctccgagat ccgtgcaggc 300

gcgaaccaca tcgcaatttt cgcgatgagc aacatctcac agccggatta cgctgcgctg 360

ctgctggacc atcgcggtat gtgcagcttt gaccgcgtct ttccgagcgg ttgttacggc 420

acccgtaagc ctgagctgag cttctacaat aaagtgctgc gtgaaattga caccccgccg 480

gaaaatgtta ttttcgttga cgatcaattg gaaaatgtga ttagcgcgca aagcattggt 540

attcatggca ttgcgtatac gaatgccgcg gaactgggcc gccagctgag aaacctgatc 600

ttcgatccgg tggagcgcgg tcgtgagttc ctgcgtcgta acgctggtga gtttcactct 660

attacggaaa cggaccagat tgtgcgcgag aacttcagcc agctgctgat tctggaagcg 720

accggtgaca aaagcctggt tagcctggaa taccaccaaa agtcgtggaa cttcttccaa 780

ggtaacccaa tcctgacgac ggaaaccttc ccggacgatg ttgacactac tagcctggct 840

ctgatgacgc tgccgacgga caccaagacc gcgaatctgt tgctggacca gattctgggt 900

ttggttaatg ccgatgaaat tgtgactacg tacttcgacc agacccgtga gcgtatcgat 960

ccagtggtct gtgtgaatgt cctgcgcctg ttctgtacgt acggccgcgg catcgcgctg 1020

ccgctgaccc tgcaatgggt ctacgatgtg ctggcgcacc gcgcatacat taacggtacg 1080

cgttattaca ccagcccgga gagctttctg tattttgtcg gtcagctctg tcgttttagc 1140

accggtgtgc tggcactgcg tccgctggag actctgctga ttgatcgtct gaaagagcgc 1200

ctgcaagtta aagctgaccc gctgagcctg gcaatgcgca tccttacgtg cttatctgtc 1260

ggtgtcagcc aggttgaagt ggacttgcgt gagttgttga gcatgcagtg cgaggacggt 1320

agctgggagc attgcccgtt cacccgctac ggcctgagca aggtttccat cggtaaccgt 1380

ggcctgacca cggcgtttgt ggttaaagcc gtcgagatgt gccgtggcag ctaa 1434

<210> 78

<211> 528

<212> PRT

<213> 热焦曲霉(Aspergillus calidoustus)_CEN60542.1蛋白

<400> 78

Met Val Arg Ala Leu Ile Leu Asp Leu Gly Asp Val Leu Phe Asn Trp

1 5 10 15

Asp Ala Pro Ala Ser Thr Pro Ile Ser Arg Lys Thr Leu Gly Gln Met

20 25 30

Leu His Ser Glu Ile Trp Gly Glu Tyr Glu Arg Gly His Leu Thr Glu

35 40 45

Asp Glu Ala Tyr Asn Ala Leu Ala Lys Arg Tyr Ser Cys Glu Ala Lys

50 55 60

Asp Val Ala His Thr Phe Val Leu Ala Arg Glu Ser Leu Arg Leu Asp

65 70 75 80

Thr Lys Phe Lys Thr Phe Leu Gln Thr Leu Lys Gln Asn Ala Asn Gly

85 90 95

Ser Leu Arg Val Tyr Gly Met Ser Asn Ile Ser Lys Pro Asp Phe Glu

100 105 110

Val Leu Leu Gly Lys Ala Asp Asp Trp Thr Leu Phe Asp Lys Ile Phe

115 120 125

Pro Ser Gly His Val Gly Met Arg Lys Pro Asp Leu Ala Phe Phe Arg

130 135 140

Tyr Val Leu Lys Asp Ile Ser Thr Pro Val Glu Asp Val Val Phe Val

145 150 155 160

Asp Asp Asn Leu Asp Asn Val Thr Ser Ala Arg Ser Leu Gly Met Arg

165 170 175

Ser Val Leu Phe His Lys Lys Asp Glu Val Gln Arg Gln Leu Thr Asn

180 185 190

Ile Phe Gly Ser Pro Ala Glu Arg Gly Leu Glu Tyr Leu Ser Ala Asn

195 200 205

Lys Thr Asn Leu Gln Ser Ala Thr Thr Thr Asp Ile Pro Ile Gln Asp

210 215 220

Asn Phe Gly Gln Leu Leu Ile Leu Glu Ala Thr Glu Asp Pro Ser Leu

225 230 235 240

Val Arg Met Glu Pro Gly Lys Arg Thr Trp Asn Phe Phe Ile Gly Ser

245 250 255

Pro Ser Leu Thr Thr Asp Thr Phe Pro Asp Asp Leu Asp Thr Thr Ser

260 265 270

Leu Ala Leu Ser Ile Val Pro Thr Ser Pro Asp Val Val Asn Ser Val

275 280 285

Ile Asp Glu Ile Ile Ser Arg Arg Asp Lys Asp Gly Ile Val Pro Thr

290 295 300

Tyr Phe Asp Asn Thr Arg Pro Arg Val Asp Pro Ile Val Cys Val Asn

305 310 315 320

Val Leu Ser Met Phe Ala Lys Tyr Gly Arg Glu His Asp Leu Pro Ala

325 330 335

Thr Val Ala Trp Val Arg Asp Val Leu Tyr His Arg Ala Tyr Leu Gly

340 345 350

Gly Thr Arg Tyr Tyr Gly Ser Ala Glu Ala Phe Leu Phe Phe Phe Thr

355 360 365

Arg Phe Val Arg Asn Leu Arg Pro Gly Thr Leu Lys Gln Asp Leu His

370 375 380

Ala Leu Leu Ser Glu Arg Val Arg Glu Arg Leu Asn Thr Pro Val Asp

385 390 395 400

Ala Leu Ala Leu Ser Met Arg Ile Gln Ala Cys His Ala Leu Gly Phe

405 410 415

Asp Ala Pro Ala Asp Ile Ala Thr Leu Ile Thr Met Gln Asp Glu Asp

420 425 430

Gly Gly Trp Pro Ala Ala Val Ile Tyr Lys Tyr Gly Ala Gly Gly Leu

435 440 445

Gly Ile Thr Asn Arg Gly Val Ser Thr Ala Phe Ala Val Lys Ala Ile

450 455 460

Thr Gly Ser Pro Val Lys Thr Glu Thr Asn Ile Gly Gly Asp Gly Ala

465 470 475 480

Arg Ala Val Ser Ala Met Ser Ser Leu Glu Ala Arg Arg Leu Gln Pro

485 490 495

Ile Ser Ser Val Gly Asp Trp Val Arg Phe Ile Ile Ala Ser Leu His

500 505 510

Val His Leu Ala Trp Leu Trp Asn Val Leu Leu Leu Ser Lys Val Val

515 520 525

<210> 79

<211> 1587

<212> DNA

<213> 热焦曲霉(Aspergillus calidoustus)_CEN60542.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 79

atggtccgcg cactcatcct cgatctcggc gatgtcctct tcaactggga cgcgcctgcg 60

tccaccccca tttcacgcaa gaccctcggc cagatgctgc atagtgagat ctggggtgag 120

tatgaacgtg gccatttgac agaagacgag gcatacaacg cactcgcgaa gcggtattcc 180

tgcgaggcca aggatgtcgc acataccttt gtcctggcac gagaatcgct gcggctcgac 240

acgaaattca aaacgtttct gcagactcta aagcagaatg ccaacggctc ccttcgtgtc 300

tatggcatgt cgaatatatc gaaaccggat ttcgaagtcc tgctgggcaa ggccgatgac 360

tggactctgt ttgacaagat cttcccctct ggccatgtcg gtatgcgcaa gccagatctt 420

gccttcttcc gctatgtgct caaggacatt tcaacgcctg tcgaggatgt ggtgtttgtt 480

gacgataacc tggacaacgt gacgagtgct cggtctctgg gcatgcgcag cgtcctcttt 540

cataagaaag acgaggtcca gcgacagctc accaacatct ttggcagccc tgctgagcgg 600

ggcttggagt atctctccgc caacaagacg aatctgcaga gtgctaccac gacagatatc 660

ccaatccagg ataactttgg ccaacttctg attctcgagg ccactgaaga cccatcgctg 720

gtccgcatgg agcccggtaa gcgaacctgg aatttcttca tcggttctcc atccctcaca 780

accgacacct tccccgacga tctcgacacc acatcccttg ccctctccat cgtacccaca 840

agccccgacg tcgtcaactc ggtcatcgac gagattatca gccgtcgcga caaggacggt 900

atcgtcccga cttacttcga caacacccgc ccccgcgtgg acccaatcgt ctgcgtaaac 960

gtcctctcca tgttcgcaaa gtacggccgc gagcacgacc tccccgcaac agttgcgtgg 1020

gtccgcgacg tcttgtatca tcgagcatac ctcggcggaa cacggtacta cgggtcagct 1080

gaggccttcc tcttcttctt cactcgcttc gttcgcaacc tccgaccggg aactctcaag 1140

caggatctac acgcattgct atcagagcgc gtgcgcgagc gactcaatac ccccgtcgac 1200

gcactcgccc tgtcaatgcg catccaggcc tgtcatgcgc tgggctttga cgcccccgca 1260

gacattgcga cgctcatcac aatgcaggac gaggacggcg ggtggccggc agccgtcatc 1320

tacaagtacg gggccggggg gttggggatc acgaaccggg gtgtttcgac tgcgtttgcc 1380

gtaaaggcga ttacagggtc gcccgtgaag actgaaacca acataggcgg cgatggagct 1440

cgcgctgtct cggccatgtc ctccttggag gcgaggaggc tacagccgat ctcgtcggtt 1500

ggggactggg tgcggtttat cattgcgtcg ttgcatgtcc atctggcttg gctttggaat 1560

gttttgcttt tgagcaaggt tgtttga 1587

<210> 80

<211> 1587

<212> DNA

<213> 人工序列_CEN60542.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 80

atggttcgtg cgttgatttt ggatttgggt gatgtgttgt ttaattggga cgcccctgca 60

agcactccga tcagccgtaa gaccctgggc cagatgctgc attccgagat ttggggtgag 120

tatgagcgtg gtcacctgac cgaagatgaa gcgtacaacg cgctggcaaa gcgctacagc 180

tgcgaggcaa aagacgtggc gcatactttt gttttggcgc gtgaaagcct gcgcctggat 240

accaagttta agacttttct gcagaccctg aaacagaacg cgaacggctc gctgcgtgtt 300

tatggtatgt ccaatatcag caaaccggat tttgaagtgc tgctgggtaa agctgacgac 360

tggaccttgt tcgacaagat cttcccgagc ggtcatgtcg gtatgcgcaa accggacctg 420

gctttctttc gttacgtgct gaaagacatc agcaccccgg ttgaggatgt tgtgtttgtt 480

gacgataacc tggataatgt gacgtctgcc cgttccctgg gtatgcgtag cgtcctgttc 540

cacaaaaaag acgaagtcca acgtcagctg accaacattt tcggtagccc tgctgagcgc 600

ggtctggagt atctgtccgc gaacaagacc aatctgcaaa gcgcaaccac caccgacatc 660

cctatccaag acaactttgg tcaattactg attctggaag ccaccgaaga tccgagcctg 720

gtacgcatgg aaccgggcaa gcgtacctgg aatttcttca ttggctctcc gagcctgacg 780

acggatacct tcccggatga cctggacacg acgagcctcg cactgtccat cgtgccgacc 840

agcccagatg ttgttaatag cgtgatcgat gagatcatca gccgtcgcga caaggacggt 900

attgtgccga cgtactttga taacacgcgc ccgcgtgtgg acccgattgt ttgtgttaac 960

gttctgtcta tgttcgcgaa atatggccgt gagcacgatc tgccggcgac ggtcgcgtgg 1020

gtccgcgacg tcctctatca tcgcgcatac ctgggtggca ccagatacta cggtagcgcg 1080

gaagccttcc ttttcttctt tacgcgcttt gtgcgtaatc tgcgtccggg cacgctgaaa 1140

caagatctgc acgcgttgct gagcgagcgt gtccgtgagc gcctgaatac cccggtggat 1200

gcgctggcgc tgagcatgcg cattcaggct tgccacgcac tgggctttga cgccccagct 1260

gacatcgcga cgctgattac catgcaagat gaagatggtg gctggccggc ggcagttatc 1320

tacaaatatg gtgcgggtgg cctgggcatt acgaaccgtg gtgtgtccac ggcattcgcg 1380

gtgaaggcaa tcacgggtag cccggttaaa accgaaacca acatcggcgg cgacggtgcc 1440

cgtgcagtgt cggccatgag cagcctggaa gcccgtcgtt tgcagccgat ttctagcgtc 1500

ggcgactggg tccgtttcat catcgcatca ctgcacgtcc acctggcgtg gctgtggaat 1560

gtcctgctgc tgagcaaagt cgtttaa 1587

<210> 81

<211> 531

<212> PRT

<213> 不整异担子菌(Heterobasidion irregulare)_XP_009547469.1蛋白

<400> 81

Met Ser Met Ile Pro Arg Cys Ser Asn Leu Ile Leu Asp Ile Gly Asp

1 5 10 15

Val Leu Phe Thr Trp Ser Pro Lys Thr Ser Thr Ser Ile Ser Pro Arg

20 25 30

Thr Met Lys Ser Ile Leu Ser Ser Thr Thr Trp His Gln Tyr Glu Thr

35 40 45

Gly His Ile Ser Gln Gly Asp Cys Tyr Arg Leu Ile Gly Asn Gln Phe

50 55 60

Ser Ile Asp Pro Gln Glu Val Gly Leu Ala Phe Gln Gln Ala Arg Asp

65 70 75 80

Ser Leu Gln Pro Asn Val Asp Phe Ile His Phe Ile Arg Ala Leu Lys

85 90 95

Ala Glu Ser His Gly Thr Leu Arg Val Phe Ala Met Ser Asn Ile Ser

100 105 110

Gln Pro Asp Tyr Ala Val Leu Arg Thr Lys Asp Ala Asp Trp Ala Val

115 120 125

Phe Asp Asp Ile Phe Thr Ser Ala Asp Ala Gly Val Arg Lys Pro His

130 135 140

Leu Gly Phe Tyr Lys Leu Val Leu Gly Lys Ile Gly Ala Asp Pro Asn

145 150 155 160

Asp Thr Val Phe Val Asp Asp Lys Gly Asp Asn Val Leu Ser Ala Arg

165 170 175

Ser Leu Gly Leu His Gly Ile Val Phe Asp Ser Met Asp Asn Val Lys

180 185 190

Arg Ala Leu Arg Tyr Leu Ile Ser Asp Pro Ile Arg Arg Gly Arg Glu

195 200 205

Phe Leu Gln Ala Arg Ala Gly His Leu Glu Ser Glu Thr Asn Thr Gly

210 215 220

Ile Glu Ile Gly Asp Asn Phe Ala Gln Leu Leu Ile Leu Glu Ala Thr

225 230 235 240

Lys Asp Arg Thr Leu Val Asn Tyr Met Asp His Pro Asn Lys Trp Asn

245 250 255

Phe Phe Arg Asp Gln Pro Leu Leu Thr Thr Glu Glu Phe Pro Phe Asp

260 265 270

Leu Asp Thr Thr Ser Ile Gly Thr Leu Ala Thr Gln Arg Asp Asp Gly

275 280 285

Thr Ala Asn Leu Val Met Asp Glu Met Leu Gln Tyr Arg Asp Glu Asp

290 295 300

Gly Ile Ile Gln Thr Tyr Phe Asp His Glu Arg Pro Arg Ile Asp Pro

305 310 315 320

Ile Val Cys Val Asn Val Leu Ser Leu Phe Tyr Ser Arg Gly Arg Gly

325 330 335

Ser Glu Leu Ala Pro Thr Leu Glu Trp Val Arg Gly Val Leu Lys His

340 345 350

Arg Ala Tyr Leu Asp Gly Thr Arg Tyr Tyr Glu Thr Gly Glu Cys Phe

355 360 365

Leu Phe Phe Leu Ser Arg Leu Leu Gln Ser Thr Lys Asp Ala Ala Leu

370 375 380

His Ala Ser Leu Lys Ser Leu Phe Ala Glu Arg Val Lys Glu Arg Ile

385 390 395 400

Gly Ala Pro Gly Asp Ala Leu Ala Leu Ala Met Arg Ile Leu Ala Cys

405 410 415

Ala Ala Val Gly Val Arg Asp Glu Ile Asp Leu Arg Ser Leu Leu Pro

420 425 430

Leu Gln Cys Glu Asp Gly Gly Trp Glu Ala Gly Trp Val Tyr Lys Tyr

435 440 445

Gly Ser Ser Gly Val Lys Ile Gly Asn Arg Gly Leu Thr Thr Ala Leu

450 455 460

Ala Leu Asn Ala Ile Glu Ala Val Glu Gly Arg Arg Thr Arg Pro Lys

465 470 475 480

Ser Gly Lys Ile Ser Arg Val Ser Arg His Ser Glu Val Ala Ala Ala

485 490 495

Pro Arg Ser Ser Thr Ser Ser His Arg Ser Asn Arg Ser Ile Ser Arg

500 505 510

Thr Phe Gln Ala Tyr Phe Lys Ala Ser Trp Thr Ser Met Lys Gln Val

515 520 525

Ala Val Ala

530

<210> 82

<211> 1596

<212> DNA

<213> 不整异担子菌(Heterobasidion irregulare)_XP_009547469.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 82

atgtccatga tacccagatg ctcgaatctc atcctcgaca tcggggatgt tctcttcaca 60

tggtctccga agacgtccac ttcgatctcc ccccgcacca tgaagagcat actgtcatcg 120

acgacctggc accaatacga gaccgggcac atttcacagg gcgactgcta ccgcctcata 180

ggcaaccagt tctccatcga tcctcaggaa gtcggacttg cattccaaca agctcgggac 240

tcattgcagc ctaatgttga cttcattcac ttcatccgcg ccctcaaggc ggaatcacac 300

gggacgctgc gcgtcttcgc tatgtccaac atctctcagc ccgattacgc agttcttcgg 360

actaaggacg ccgactgggc cgtttttgac gatatattca cgtctgcaga tgctggggtt 420

cgaaagccac accttgggtt ctacaagttg gtactcggaa agatcggcgc cgatccaaac 480

gataccgtct tcgtcgatga caagggggac aatgtcctct ctgcacggtc tctcggcctt 540

catggaatcg tctttgacag tatggacaac gtcaagcgag ccctgcgcta cttgatcagc 600

gaccccatac ggcgaggacg agagtttctc caagcgcgag ccggccattt ggagtcggag 660

accaatacgg gcatcgaaat cggtgataat tttgcccagc tccttattct cgaggccacg 720

aaggatagga cactcgtcaa ttatatggac catccgaaca aatggaattt cttccgagat 780

caaccgctcc tcacaacgga ggagttccct ttcgatctcg atacgacatc tattggaacg 840

cttgcgacgc agcgcgatga tgggactgcc aatctagtaa tggatgagat gcttcagtac 900

cgtgatgagg atggcataat acaaacatat ttcgatcatg aacgaccgag gatagatccc 960

atcgtctgtg tcaacgtctt gagccttttc tactcccggg gtcgtggttc ggagctagca 1020

ccgacactag agtgggtgcg tggtgtcctc aagcaccgcg cgtatctcga tggaacgcga 1080

tactacgaga caggcgaatg cttccttttc ttcctcagcc ggctcttgca atcaaccaag 1140

gacgccgcct tgcacgcatc gttgaaatct ttgttcgccg aacgggtcaa ggagcgcata 1200

ggggcaccag gggacgcgct ggcgctggcg atgcgtatac tggcatgcgc agcagtgggc 1260

gtgcgggacg agatcgatct tcgatcacta ttacctctgc agtgcgagga tggggggtgg 1320

gaggcaggct gggtgtacaa gtatgggtct tcgggagtca agatcggcaa tcgtggcctc 1380

acgactgcgc ttgcgctcaa tgccatcgag gctgtggagg gacgtcgcac gaggccgaag 1440

tcgggtaaga tcagccgagt cagccgtcat tctgaggtcg cagcagcgcc acggtcttcc 1500

accagcagtc atcgttctaa tcgctcgatc tcaaggacat tccaggcgta cttcaaggcg 1560

tcgtggacat cgatgaaaca ggtggccgtg gcgtga 1596

<210> 83

<211> 1596

<212> DNA

<213> 人工序列_XP_009547469.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 83

atgagcatga ttccacgttg tagcaatctg attctcgaca tcggtgatgt gttgtttacg 60

tggagcccga aaaccagcac cagcattagc ccgcgtacca tgaaatctat cctgagctct 120

accacctggc atcaatatga gactggccac atcagccagg gtgattgcta ccgcctgatc 180

ggtaatcagt tctccatcga cccgcaagag gtcggtttgg ccttccagca agccagagac 240

agcctgcaac cgaatgttga tttcatccat ttcattcgtg ccctgaaagc tgagtcgcac 300

ggcaccctgc gcgtttttgc gatgagcaat atcagccaac ctgactatgc agtcctgcgt 360

acgaaagacg cggactgggc tgtttttgat gatatcttca cgagcgcgga tgctggtgtt 420

cgtaaaccgc acctgggttt ttataaactg gtcttaggca agattggcgc ggaccctaac 480

gacaccgttt ttgtggatga taagggtgac aacgtcctct ctgcacgttc cctgggtctg 540

cacggtatcg tttttgattc aatggacaac gtgaagcgcg cactgcgcta cctgattagc 600

gacccgatcc gccgcggccg tgaatttctg caggcccgtg cgggtcacct ggagtccgaa 660

acgaacacgg gtattgagat tggtgataat ttcgcgcaat tgctgatcct ggaagcgacc 720

aaagatcgta ctctggtgaa ctacatggac cacccgaaca agtggaactt cttccgtgac 780

cagccgctgc tgaccaccga agaatttccg ttcgacctgg acacgaccag cattggcacg 840

ctggccaccc aacgtgacga tggtacggcg aatctggtaa tggacgaaat gttgcagtat 900

cgtgacgaag atggcatcat tcagacctat ttcgatcatg agcgcccgcg tattgatccg 960

attgtttgtg tgaatgtgct gtctctgttc tacagccgtg gccgtggctc tgagttggcg 1020

ccgacgctgg aatgggtgcg cggtgtgttg aaacatcgtg cgtacctgga tggtacgcgt 1080

tattacgaga ctggtgagtg tttcctgttt ttcctgagcc gtctgctgca gagcaccaaa 1140

gacgcagccc tgcacgcgag cctgaagtcc ctgtttgcag agcgtgttaa agagcgcatc 1200

ggtgcgccgg gcgatgctct ggcgctggct atgcgcatcc tggcgtgcgc cgctgttggt 1260

gtgcgcgatg aaattgattt gcgtagcctg ctgccgctgc aatgcgaaga tggcggctgg 1320

gaagcgggct gggtctacaa atacggcagc agcggtgtga agattggcaa tcgcggtctt 1380

accacggcgc tggcattgaa tgctatcgaa gccgttgagg gccgtcgcac ccgcccaaag 1440

tccggtaaga tcagccgtgt tagccgtcat agcgaagtcg cagcggcacc gcgttcctcg 1500

acgagcagcc accgtagcaa ccgtagcatt agccgcacct tccaggcata ttttaaagcg 1560

agctggacca gcatgaaaca agtcgcagtg gcgtaa 1596

<210> 84

<211> 518

<212> PRT

<213> 近光彩裂孔菌(Schizopora paradoxa)_KLO09124.1蛋白

<400> 84

Met Ser Ile His Gly Ser Ser Met Ser Ser Tyr Ser Ser Thr Val Pro

1 5 10 15

Ser Met Thr Ser Ser Pro Ala Ser Thr Ser Thr Pro Ser Ser Pro Ala

20 25 30

Ser Ser Ile His Glu Ile Gly Pro Val Pro Glu Ala Arg Arg Lys Gly

35 40 45

Gln Cys Asn Ala Leu Ile Phe Asp Leu Gly Asp Val Leu Phe Thr Trp

50 55 60

Ser Ala Glu Thr Lys Thr Thr Ile Ser Pro Lys Leu Leu Lys Lys Ile

65 70 75 80

Leu Asn Ser Leu Thr Trp Phe Glu Tyr Glu Lys Gly Asn Ile Gly Glu

85 90 95

Gln Glu Ala Tyr Asp Ala Val Ala Lys Glu Phe Gly Val Pro Ser Ser

100 105 110

Glu Val Gly Ala Ala Phe Gln Cys Ala Arg Asp Ser Leu Gln Ser Asn

115 120 125

Pro Arg Leu Val Ser Leu Ile Arg Glu Leu Lys Ser Gln Tyr Asp Leu

130 135 140

Lys Val Tyr Ala Met Ser Asn Ile Ser Ala Pro Asp Trp Glu Val Leu

145 150 155 160

Arg Thr Lys Ala Thr Pro Glu Glu Trp Ala Met Phe Asp Arg Val Phe

165 170 175

Thr Ser Ala Ala Ala Arg Glu Arg Lys Pro Asn Leu Gly Phe Tyr Arg

180 185 190

Gln Val Val Glu Ala Thr Gly Val Asp Pro Ala Arg Ser Val Phe Val

195 200 205

Asp Asp Lys Leu Asp Asn Val Ile Ser Ala Arg Ser Val Gly Leu Asn

210 215 220

Ala Ile Ile Phe Asp Ser Phe Glu Asn Val Ala Arg Gln Leu Lys Asn

225 230 235 240

Tyr Val Ala Asp Pro Ile Gly Arg Ala Glu Ala Trp Leu Arg Asp Asn

245 250 255

Ala Lys Lys Met Leu Ser Ile Thr Asp Ala Gly Val Val Val Tyr Glu

260 265 270

Asn Phe Gly Gln Met Leu Ile Leu Glu Ala Thr Gly Asp Arg Ser Leu

275 280 285

Val Asp Tyr Val Glu Tyr Pro Arg Leu Phe Asn Phe Phe Gln Gly Asn

290 295 300

Gly Val Phe Thr Thr Glu Ser Phe Pro Cys Asp Leu Asp Ser Thr Ser

305 310 315 320

Ile Gly Leu Thr Val Thr Asn His Val Asp Glu Lys Thr Arg His Ser

325 330 335

Val Met Asp Glu Met Leu Thr Tyr Lys Asn Glu Asp Gly Ile Ile Ala

340 345 350

Thr Tyr Phe Asp Ala Thr Arg Pro Arg Ile Asp Pro Val Val Cys Ala

355 360 365

Asn Val Leu Thr Phe Phe Tyr Lys Asn Gly Arg Gly Glu Glu Leu Asn

370 375 380

Glu Thr Leu Asp Trp Val Tyr Asp Ile Leu Leu His Arg Ala Tyr Leu

385 390 395 400

Asp Gly Thr Arg Tyr Tyr Phe Gly Ser Asp Thr Phe Leu Phe Phe Leu

405 410 415

Ser Arg Leu Leu Ser Glu Ser Pro Ser Val Tyr Ala Arg Phe Ala Pro

420 425 430

Val Phe Gln Glu Arg Val Lys Glu Arg Met Gly Ala Thr Gly Asp Ala

435 440 445

Met Ser Leu Ala Met Arg Ile Ile Ala Ala Ala Thr Val Lys Ile Gln

450 455 460

Asp Arg Val Asp Cys Asp Ala Leu Leu Gln Thr Gln Glu Asp Asp Gly

465 470 475 480

Gly Phe Pro Ile Gly Trp Met Tyr Lys Tyr Gly Ala Thr Gly Met Leu

485 490 495

Leu Gly Asn Lys Gly Leu Ser Thr Ala Leu Ala Ile Gln Ala Ile Lys

500 505 510

Ala Val Glu Ser Phe Pro

515

<210> 85

<211> 1557

<212> DNA

<213> 近光彩裂孔菌(Schizopora paradoxa)_KLO09124.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 85

atgtcgattc acggttcttc tatgtcctcc tattcctcga ctgtgccgtc aatgacttcc 60

tctcccgcgt ccacttctac tccgtcgtct cctgcatcgt cgatccatga gattggtcct 120

gtcccagaag ctcgacgaaa gggacagtgc aacgcgctga tcttcgacct cggagacgtc 180

ctcttcacct ggtcggcaga gactaagacc accatttccc cgaaactcct gaaaaagatc 240

cttaactcct taacatggtt cgaatacgag aagggaaaca tcggggagca ggaggcgtat 300

gacgcagtcg caaaggagtt tggcgtcccg tcgtccgagg tcggggccgc tttccagtgc 360

gcgcgcgatt cgctacagag caatccccgc ctcgtctcgc tcatccgtga gctgaagtcg 420

caatatgatc tcaaggtgta cgccatgtcc aacatctctg cgccggactg ggaagtccta 480

aggacgaagg cgacccctga ggagtgggca atgtttgacc gcgtcttcac gagcgcggcc 540

gcgcgcgagc gtaagccaaa cctcggattc tacagacagg ttgttgaggc gaccggcgtc 600

gaccccgctc gctccgtgtt cgtcgacgat aaactcgaca atgtcatctc tgcgcgttca 660

gtcggattaa atgcgatcat cttcgactca tttgagaacg tcgcccggca gctcaaaaac 720

tatgtcgctg atcctatcgg acgggcggag gcgtggttgc gcgataacgc aaagaagatg 780

ttgtcaatta cggatgccgg ggtggtcgta tacgagaatt tcggccagat gctgatcttg 840

gaggcaacag gcgataggtc gcttgtggac tacgtcgagt accctcgtct cttcaacttc 900

ttccaaggca atggcgtctt tacgaccgag tcattccctt gcgaccttga ttcgacttcc 960

atcggcttaa ccgtcacgaa ccacgtcgat gagaaaacaa ggcacagcgt catggatgag 1020

atgctgacct acaaaaatga ggatggtatc attgcgactt actttgatgc cacgcgtccc 1080

cgaattgacc ccgtcgtctg cgccaatgtc ttgacgttct tctacaagaa cggccgaggg 1140

gaggagctca atgaaacact tgactgggtc tacgacatcc tccttcatcg cgcgtacctc 1200

gatggcacac gctattattt cggctcagac accttcctct tcttcctttc tcgacttctc 1260

tccgaatcgc catccgttta cgcccgtttc gctccggtgt tccaggagag agtcaaggag 1320

cgcatggggg cgacgggaga tgcgatgtcc cttgcgatgc gcatcatcgc ggccgcaact 1380

gtcaagatcc aagaccgagt cgactgcgac gctctgctgc agacgcagga agacgacggt 1440

ggattcccga taggttggat gtacaagtac ggggcgaccg ggatgcttct gggtaacaag 1500

ggcttgtcga cagctctggc aatccaagct atcaaagcgg tcgaatcttt cccttga 1557

<210> 86

<211> 1557

<212> DNA

<213> 人工序列_KLO09124.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 86

atgtcgattc acggtagcag catgtcgtct tatagcagca cggttccatc tatgactagc 60

agcccggctt ccacgagcac gccgtccagc ccggccagca gcatccacga aatcggcccg 120

gtccctgagg cgcgtcgcaa gggccaatgc aatgcactga tcttcgacct gggtgatgtt 180

ctgtttacct ggagcgcaga aaccaagacc acgatcagcc cgaagctgct gaaaaagatt 240

ctgaacagct tgacctggtt tgagtatgag aaaggcaaca tcggtgaaca agaagcctat 300

gacgccgttg cgaaagagtt cggtgtgccg agctctgagg ttggcgctgc gtttcaatgt 360

gcgcgtgact ccctgcaaag caatccgcgt ttggttagcc tgattcgtga gctgaagtcc 420

cagtacgacc tgaaagtgta cgctatgagc aatattagcg cgccagactg ggaagtgctg 480

cgtactaaag cgaccccgga agagtgggca atgttcgatc gtgtctttac ttctgcggcg 540

gcgcgtgagc gtaagccgaa cttgggcttt taccgccaag tcgtggaagc aaccggtgtc 600

gatccggcgc gtagcgtttt cgtcgatgat aaactggaca atgtgatcag cgcgcgctct 660

gtcggtctga acgctattat cttcgactcc ttcgaaaacg tcgcccgtca gctgaagaat 720

tacgtcgcag acccgattgg tcgcgctgag gcgtggctgc gcgacaacgc aaagaaaatg 780

ctgagcatca ccgatgcggg tgttgtggtt tacgagaatt ttggccagat gctgatcctg 840

gaagctaccg gtgaccgtag cctggtggac tatgtggagt atccgcgcct ctttaacttc 900

ttccagggta acggcgtttt tacgaccgag agctttccat gcgatctgga cagcaccagc 960

atcggtctga ctgtgaccaa tcatgtggac gaaaagactc gccacagcgt catggacgaa 1020

atgctgacct acaaaaatga agatggtatt attgcgacgt actttgacgc gacgcgcccg 1080

cgcattgacc ctgttgtctg tgccaatgtt ctgaccttct tctacaaaaa cggtcgtggt 1140

gaagaattga acgaaaccct ggattgggtg tacgacattc tgctgcatcg cgcgtatctg 1200

gacggtacgc gttattattt cggctccgat acgttcctgt ttttcctgag ccgtctgctg 1260

agcgagtctc cgagcgttta cgcgcgtttt gccccggtgt ttcaagagcg cgtgaaagag 1320

cgtatgggcg cgaccggtga tgcgatgagc ctggccatgc gtatcattgc agcagcaacc 1380

gtaaagatcc aggatcgtgt ggattgcgac gcactgttgc agacccaaga agatgatggc 1440

ggtttcccga ttggttggat gtacaaatat ggtgcgaccg gtatgttgct gggcaacaaa 1500

ggcctgagca cggccctggc gatccaggca attaaagccg tcgagtcgtt cccgtaa 1557

<210> 87

<211> 507

<212> PRT

<213> 杂色曲霉(Aspergillus versicolor)_OJI95797.1蛋白

<400> 87

Met Gly Ser Thr Lys Ala Leu Val Val Asp Phe Gly Asn Val Leu Cys

1 5 10 15

Thr Trp Thr Pro Pro Arg Glu Leu Ser Ile Pro Pro Lys Lys Leu Lys

20 25 30

Gln Ile Met Ser Ser Asp Ile Trp Leu Asp Tyr Glu Arg Gly Ile Tyr

35 40 45

Lys Ser Glu Asp Glu Cys Tyr Leu Ala Val Ala Thr Arg Phe Gly Val

50 55 60

Ser Pro Ser Asp Leu Ser Ser Val Met Lys Lys Ala Arg Glu Ser Leu

65 70 75 80

Gln Pro Asn Thr Ala Thr Leu Asn His Leu Ser His Leu Lys Lys Thr

85 90 95

Gln Pro Gly Leu Arg Ile Tyr Gly Leu Thr Asn Thr Pro Leu Pro Glu

100 105 110

Gln Ser Ser Val Arg Ser Ile Ala Gln Glu Trp Pro Ile Phe Asp His

115 120 125

Ile Tyr Ile Ser Gly Ile Leu Gly Met Arg Lys Pro Asp Ile Gly Cys

130 135 140

Tyr Arg Leu Val Leu Arg Lys Ile Gly Leu Pro Ala Glu Ser Val Val

145 150 155 160

Phe Ile Asp Asp Ser Pro Glu Asn Ile Leu Ala Ala Gln Ser Leu Gly

165 170 175

Val His Ser Ile Leu Phe Gln Ser His Asp Gln Leu Ser Arg Gln Leu

180 185 190

Gly Asn Val Leu Gly Asp Pro Ile Gln Arg Gly His Asn Phe Leu Leu

195 200 205

Ser Asn Ala Lys Gln Met Asn Ser Thr Thr Asp Lys Gly Val Ile Ile

210 215 220

Arg Asp Asn Phe Ala Gln Leu Leu Ile Ile Glu Leu Thr Gln Asn Pro

225 230 235 240

Asp Leu Val Ala Leu Glu Thr Trp Asp Arg Thr Trp Asn Phe Phe Ile

245 250 255

Gly Pro Pro Gln Leu Thr Thr Glu Ser Phe Pro Asn Asp Leu Asp Thr

260 265 270

Thr Ser Ile Ala Leu Ser Val Leu Pro Val Asp Lys Glu Val Val Trp

275 280 285

Ser Val Met Asp Glu Met Leu Thr Phe Thr Asn Ala Asp Gly Ile Phe

290 295 300

Met Thr Tyr Phe Asp Arg Ser Arg Pro Arg Val Asp Pro Val Val Cys

305 310 315 320

Thr Asn Val Leu Asn Leu Phe Cys Met His Gly Arg Glu Ser Glu Val

325 330 335

Ala Ala Thr Phe Asp Trp Val Leu Asp Val Leu Arg Asn Ser Ala Tyr

340 345 350

Leu Ser Gly Ser Arg Tyr Tyr Ser Ser Pro Asp Cys Phe Leu Tyr Phe

355 360 365

Leu Ser Arg Leu Ser Cys Val Val Arg Asp Gly Thr Arg Arg Arg Glu

370 375 380

Leu Lys Ser Leu Leu Lys Gln Gln Val Ser Gln Arg Ile Gly Ala Asp

385 390 395 400

Gly Asp Ser Val Ser Leu Ala Thr Arg Leu Leu Ala Ser Asn Ile Leu

405 410 415

Gly Ile Thr Asn Gly Arg Asp Arg Ser Arg Leu Leu Ala Leu Gln Glu

420 425 430

Thr Asp Gly Gly Trp Pro Ala Gly Trp Val Tyr Lys Phe Gly Ser Ser

435 440 445

Gly Val Gln Ile Gly Asn Arg Gly Leu Ser Thr Ala Leu Ala Leu Lys

450 455 460

Ser Ile Glu Arg Gln Lys Gly Pro Val Glu Ala Ile Ser Ser Glu Pro

465 470 475 480

Glu Ala Trp Trp Pro Ser Leu Arg Leu Asp Arg Leu Leu Asn Val Trp

485 490 495

Pro Phe Ile Asp Trp Lys Gly Tyr Ser Pro Ser

500 505

<210> 88

<211> 1524

<212> DNA

<213> 杂色曲霉(Aspergillus versicolor)_OJI95797.1 cDNA

<220>

<221> misc_feature

<223> cDNA

<400> 88

atgggttcca ccaaggctct tgttgttgac tttgggaatg ttttgtgtac ctggacacca 60

cccagggagt tatccatccc gcccaagaag ctgaaacaaa tcatgtcttc tgacatttgg 120

ctcgactatg aacggggtat ctataagtcg gaggacgagt gctacttggc ggttgcaact 180

cgcttcggcg tctctcccag cgacctctcc tcggtgatga aaaaggcccg cgagagcctg 240

caaccaaaca ccgcaaccct gaatcatctg tctcatctca aaaagaccca gcctggcctc 300

aggatatacg gtttgaccaa cacccctctc ccagaacaaa gcagtgtacg atccatcgcc 360

caggaatggc ctatcttcga ccatatctac atatcaggca tcctcggaat gcgcaagccg 420

gacattggct gctacaggct ggtgctgcga aagattgggc ttccagcgga gtccgtggtc 480

ttcattgatg attcacccga gaacatcctg gccgcgcagt cactgggagt acacagcata 540

ctgttccaaa gccacgacca gctctctcgt cagcttggca atgtgctggg tgatccaatc 600

cagcggggcc ataacttcct actctcgaac gcaaagcaaa tgaatagtac gaccgacaag 660

ggagttatta tccgggacaa ctttgcgcaa ctgctgatca tcgagctgac gcagaaccca 720

gaccttgtgg cgttagaaac atgggaccgt acctggaatt tttttattgg acctccacaa 780

ttgacaactg aaagctttcc caatgatctt gacactacct ccatcgctct ctcggttctt 840

ccggttgaca aagaagtggt atggtctgtg atggacgaga tgctaacgtt taccaatgcg 900

gatgggattt ttatgaccta tttcgaccga tcacgccctc gagttgatcc ggtagtttgc 960

accaatgtcc tgaatctttt ctgcatgcat ggacgggaaa gcgaagttgc agccacattt 1020

gactgggtgc tggacgttct tcgaaattcg gcctatttat caggatccag atactattct 1080

tcgcctgatt gctttctata ctttctttca cggctgagct gtgtggtccg agacggcacg 1140

cgacgcaggg agctcaagtc actgttgaaa caacaagtga gccagcgtat tggcgctgat 1200

ggtgattccg tctctctcgc cactaggcta cttgcatcga acattttagg aatcacaaat 1260

ggccgtgatc gctccaggct tcttgctctg caggaaactg acggtggatg gcctgctggg 1320

tgggtttata aattcggaag ctcgggggta cagattggca atcgggggct cagtacagcc 1380

ttggcgttaa aatcaattga gcgtcagaag gggcctgttg aggcgatatc cagtgagcca 1440

gaagcgtggt ggccatccct caggcttgac cgacttctca acgtttggcc tttcatcgac 1500

tggaagggat attcgccgag ttga 1524

<210> 89

<211> 1524

<212> DNA

<213> 人工序列_OJI95797.1 优化的cDNA

<220>

<223> 密码子优化的cDNA

<400> 89

atgggttcta cgaaagcgtt ggttgttgat tttggtaatg ttctgtgcac ttggacgcca 60

ccacgtgaat tgtccatccc gccgaagaaa ctgaagcaaa tcatgagcag cgacatttgg 120

ctggactatg agcgtggtat ctacaaatcg gaagatgagt gctacctggc agttgcgacg 180

cgctttggtg tcagcccgtc cgacctgagc tccgttatga aaaaagcccg tgagagcctg 240

cagccgaata ccgcaacgct gaaccacttg agccatctga agaaaaccca gcctggcctt 300

cgtatctacg gcctgacgaa caccccgttg ccggaacaga gctcagtccg tagcattgcg 360

caggaatggc cgatttttga ccacatctac attagcggca tcttgggtat gcgcaaaccg 420

gatattggtt gttaccgtct ggttctgcgt aagatcggtc tgccagcgga gtccgtcgta 480

ttcatcgacg acagcccgga gaacattctg gcagctcaat cgttgggtgt ccatagcatc 540

ctgttccagt cccacgatca gctgagccgt cagctgggca atgtgctggg tgatccgatt 600

cagcgcggtc acaacttcct cctgtccaac gcgaagcaaa tgaacagcac caccgataag 660

ggtgtgatta tccgcgacaa cttcgcccag ctgctgatta ttgagctgac ccaaaatccg 720

gatctggttg cgctggagac ttgggaccgt acgtggaatt tctttattgg tccgccgcaa 780

ctgaccaccg agagctttcc gaacgacctg gacaccacga gcattgccct gagcgtgttg 840

ccggtggata aagaagtcgt ttggtctgtg atggatgaga tgctgacctt caccaacgca 900

gacggcatct tcatgaccta tttcgatcgt agccgtccgc gtgttgaccc ggtcgtttgt 960

accaatgtcc tgaatctgtt ttgcatgcat ggtcgcgaga gcgaagtggc cgcgacgttc 1020

gactgggtgc tggacgtgct gcgcaacagc gcgtacctga gcggttcccg ttattacagc 1080

agcccggatt gttttctgta tttcctgtct cgtctgagct gcgtcgtccg tgatggcacg 1140

cgtcgtcgtg aactgaaaag cctgctgaag caacaagttt ctcaacgtat cggcgctgac 1200

ggtgattccg tcagcctggc cacccgtttg ctggcgagca acatcctggg cattactaac 1260

ggtcgtgacc gcagccgtct gctggcattg caagaaaccg atggtggctg gcctgcaggc 1320

tgggtctata agtttggtag cagcggcgtg caaattggca atcgcggtct gagcaccgcg 1380

ctggctctga agtctatcga gcgccagaaa ggtccggtgg aagcaatcag cagcgagccg 1440

gaagcgtggt ggcctagctt acgcttggac cgcttgctga atgtttggcc atttatcgac 1500

tggaagggct actccccgag ctaa 1524

<210> 90

<211> 540

<212> PRT

<213> 双孢蘑菇(Agaricus bisporus)_XP_006461126.1蛋白

<400> 90

Met Ala Pro Pro Gln Arg Pro Phe Thr Ala Ile Val Phe Asp Ile Gly

1 5 10 15

Asp Val Leu Phe Gln Trp Ser Ala Thr Thr Lys Thr Ser Ile Ser Pro

20 25 30

Lys Thr Leu Arg Ser Ile Leu Asn Cys Pro Thr Trp Phe Asp Tyr Glu

35 40 45

Arg Gly Arg Leu Ala Glu Asn Ala Cys Tyr Ala Ala Ile Ser Gln Glu

50 55 60

Phe Asn Val Asn Pro Asp Glu Val Arg Asp Ala Phe Ser Gln Ala Arg

65 70 75 80

Asp Ser Leu Gln Ala Asn His Asp Phe Ile Ser Leu Ile Arg Glu Leu

85 90 95

Lys Ala Gln Ala Asn Gly Arg Leu Arg Val Tyr Ala Met Ser Asn Ile

100 105 110

Ser Leu Pro Asp Trp Glu Val Leu Arg Met Lys Pro Ala Asp Trp Asp

115 120 125

Ile Phe Asp His Val Phe Thr Ser Gly Ala Val Gly Glu Arg Lys Pro

130 135 140

Asn Leu Ala Phe Tyr Arg His Val Ile Ala Ala Thr Asp Leu Gln Pro

145 150 155 160

His Gln Thr Ile Phe Val Asp Asp Lys Leu Glu Asn Val Leu Ser Ala

165 170 175

Arg Ser Leu Gly Phe Thr Gly Ile Val Phe Asp Glu Pro Ser Glu Val

180 185 190

Lys Arg Ala Leu Arg Asn Leu Ile Gly Asp Pro Val Gln Arg Gly Gly

195 200 205

Glu Phe Leu Val Arg Asn Ala Gly Lys Leu Gly Ser Ile Thr Arg Thr

210 215 220

Thr Ala Lys His Glu Ser Ile Pro Leu Asp Glu Asn Phe Ala Gln Leu

225 230 235 240

Leu Ile Leu Glu Ile Thr Gly Asn Arg Ala Leu Val Asn Leu Val Glu

245 250 255

His Pro Gln Thr Trp Asn Phe Phe Gln Gly Lys Gly Gln Leu Thr Thr

260 265 270

Glu Glu Phe Pro Phe Asp Leu Asp Thr Thr Ser Leu Gly Leu Thr Ile

275 280 285

Leu Lys Arg Ser Arg Glu Ile Ala Asp Ser Val Met Asp Glu Met Leu

290 295 300

Glu Tyr Val Asp Pro Asp Gly Ile Ile Gln Thr Tyr Phe Asp His Arg

305 310 315 320

Arg Pro Arg Phe Asp Pro Val Val Cys Val Asn Ala Leu Ser Leu Phe

325 330 335

Tyr Ala Tyr Gly Arg Gly Glu Gln Leu Arg Ser Thr Leu Thr Trp Val

340 345 350

His Glu Val Leu Leu Asn Arg Ala Tyr Leu Asp Gly Thr Arg Tyr Tyr

355 360 365

Glu Thr Ala Glu Cys Phe Leu Tyr Phe Met Ser Arg Leu Leu Ala Thr

370 375 380

Ser Gly Asp Pro Asp Leu His Ser Leu Leu Lys Pro Leu Leu Lys Glu

385 390 395 400

Arg Val Gln Glu Arg Ile Gly Ala Asp Gly Asp Ser Leu Ala Leu Ala

405 410 415

Met Arg Ile Leu Ala Cys Asp Phe Val Gly Ile Arg Asp Glu Val Asp

420 425 430

Leu Arg Thr Leu Leu Thr Leu Gln Cys Glu Asp Gly Gly Trp Glu Val

435 440 445

Gly Trp Met Tyr Lys Tyr Gly Ser Ser Gly Ile Ser Ile Gly Asn Arg

450 455 460

Gly Leu Ala Thr Ala Leu Ala Ile Lys Ala Val Asp Thr Met Phe Gln

465 470 475 480

Pro Gln Ile Arg Phe Ser Glu Ser Pro Thr Asp Thr Leu Val Glu Asn

485 490 495

Ala Ile His Lys Arg Arg Pro Ser Phe Ser Glu Lys Phe Leu Gly Lys

500 505 510

Arg Pro Arg Ser Gly Ser Phe Arg Lys Pro Leu Gln Trp Ile Leu Gln

515 520 525

Gly Ser Lys Leu Arg Lys Ser Val Glu Ile Gly Ser

530 535 540

<210> 91

<211> 1808

<212> DNA

<213> 双孢蘑菇(Agaricus bisporus)_XP_006461126.1 cDNA

<400> 91

atggctccgc ctcagcgacc ctttactgcg attgtctttg acatcgggga tgttctattc 60

caatggtctg caaccaccaa aacctctatc tcaccaaaga cactccgctc tattctcaac 120

tgtccgacat ggtttgacta tgaacgtgga cgcctggcag aaaacgcttg ttatgccgct 180

atctcacaag aattcaacgt caacccagac gaagttcgcg acgctttcag ccaagcgcgc 240

gactctctcc aagcaaacca cgacttcatc agtctcatcc gtgagctgaa ggcacaagca 300

aatggtcgtt tacgtgtgta cgccatgtcg aacatatctc ttcctgattg ggaagtgctg 360

cggatgaaac ctgctgattg ggatattttc gaccacgtct tcacatccgg tgcggttggg 420

gaacgcaagc ccaatctcgc cttttatcgc catgttatcg cggccaccga tctgcagcct 480

catcagacaa tatttgttga cgataagctg gagaatgttc tctcagcacg ttccctcggg 540

ttcacaggca tcgtgtttga cgagccctcc gaggtcaaac gtgcgcttcg taacctcatt 600

ggggatcctg ttcaacgagg aggtgaattc ttggttcgga atgccggaaa gcttggctct 660

atcacaagga ctactgcaaa gcacgagtca atccccctcg acgagaattt tgctcagctt 720

cttattctcg agataacggg gaacaggtgc gttagcttct tgtagggtct tctgtcgtaa 780

tactaaattt tttctggtgt ttagggcttt ggtcaacctc gttgagcatc ctcaaacgtg 840

gaatttcttc caaggtgcgc tgctaaaata aacatccagt tgcgtttcga agctcattgt 900

gggcgtcccg tcacaggcaa gggccagctg acaacagaag aatttccatt cgatctcgat 960

acaacttctc ttggtctcac gatcctcaag cgaagcaggg aaatcgccga ttcagtcatg 1020

gatgaaatgc tggagtatgt cgatcctgat ggtatcattc aggcaagttt catttatcgg 1080

cttgagaaaa taaagacaaa aacgttctga tggggggatg tttctagacg tatttcgatc 1140

atcggagacc acgttttgat ccagtcgtgt gtgtcaatgc attaagcctc ttctatgctt 1200

acggccgcgg ggagcaactg cggtcgactt tgacatgggt acatgaagtc cttctcaatc 1260

gagcctactt ggatggcaca cggtactacg aaacagccga atgcttcctc tatttcatga 1320

gccgacttct cgccacttca ggcgaccctg accttcactc ccttcttaaa cctcttctca 1380

aagaacgggt gcaagaacgc attggagctg atggagactc tcttgcactc gcaatgcgta 1440

ttctcgcctg tgatttcgtc ggaatcagag atgaagtgga tttacgcaca cttctgactt 1500

tgcaatgtga agatggaggt tgggaagtgg gttggatgta caagtatgga tcttccggta 1560

tcagtatcgg aaatcgtgga ctggccaccg cgctcgctat caaggccgtc gacacgatgt 1620

ttcaacccca aattcggttc tctgaatcac ccacagatac tttggttgaa aacgctatcc 1680

acaaacgccg tccctcattt tccgaaaaat tcctcggcaa acgtcctcgc agcggatcgt 1740

tcaggaaacc tttacagtgg atactgcaag gttccaagct tcgcaaatct gtcgaaatag 1800

gaagctaa 1808

<210> 92

<211> 1623

<212> DNA

<213> 双孢蘑菇(Agaricus bisporus)_XP_006461126.1 优化的cDNA

<400> 92

atggcaccac cgcaacgtcc gttcactgca attgttttcg atattggcga tgttttgttc 60

caatggtctg cgaccacgaa aaccagcatt agcccgaaaa ccctgcgcag cattctgaat 120

tgtccgacct ggtttgatta tgagcgcggc cgtctggcgg aaaatgcgtg ttacgctgcg 180

atcagccaag aatttaacgt caacccggac gaagttcgcg acgccttcag ccaagcgcgc 240

gacagcctgc aggcgaatca cgacttcatc agcctgattc gtgagctgaa agctcaggcg 300

aacggtcgtc tgcgtgtcta cgccatgtct aatatcagcc tgccggattg ggaagtcctg 360

cgtatgaagc cagccgattg ggacatcttt gaccatgtat ttaccagcgg tgcggtgggt 420

gagcgcaagc cgaacctggc cttttatcgt cacgtcatcg cggccacgga tctgcagccg 480

caccagacga tcttcgtgga tgacaaactg gaaaacgtgc tgtctgcgcg ctcgctgggc 540

ttcacgggta tcgtgttcga cgagccaagc gaagtcaaac gtgcgctgcg taatctgatc 600

ggcgacccgg tgcagcgtgg tggcgagttc ctggttcgta atgctggcaa actgggttct 660

atcacccgta cgaccgcaaa acatgagagc atcccgctgg atgagaattt tgcacaactg 720

ttgattctgg aaattactgg taaccgcgca ctggtcaatc tggttgagca cccgcagacg 780

tggaacttct tccagggtaa gggccagctg acgaccgaag aatttccttt tgacctggat 840

acgacgagcc tgggtctgac gatcctgaag cgtagccgcg agattgccga ctccgtcatg 900

gacgaaatgt tggaatacgt ggaccctgac ggcatcattc agacctactt cgatcatcgt 960

cgcccgcgct ttgacccggt tgtttgcgtt aatgccctga gcctgttcta tgcatacggc 1020

cgtggtgagc aactgcgttc caccttgacc tgggtgcacg aagttctgtt gaaccgtgcg 1080

tatttggatg gtacgcgtta ctatgaaacg gccgagtgct ttctgtattt catgtcccgt 1140

ctgctggcaa ccagcggtga cccggatctg cattccctgc tgaagccgtt gctgaaggaa 1200

cgcgtgcaag agcgcatcgg cgctgacggt gacagcctgg cgctggcgat gcgcattttg 1260

gcatgtgatt ttgttggcat ccgtgatgaa gtggatctgc gtaccctgct gaccttacag 1320

tgcgaggatg gcggttggga agtgggctgg atgtacaaat acggtagcag cggtattagc 1380

attggtaacc gtggtctggc aaccgcattg gcgatcaaag ctgttgacac catgtttcaa 1440

ccgcaaatcc gtttcagcga gagcccgacc gacactctgg tggagaacgc gattcacaag 1500

cgccgcccga gcttttcaga gaaattttta ggtaagcgtc cgcgttccgg ttcgttccgt 1560

aaaccgctgc aatggattct gcagggcagc aagctgcgca agagcgtcga gatcggtagc 1620

taa 1623

<210> 93

<211> 1584

<212> DNA

<213> XP_007369631.1 针对酿酒酵母表达而优化的cDNA

<400> 93

atggcttcta tccacagaag atacactact ttgatcttgg acttgggtga cgttttgttc 60

agatggtctc caaagactga aactgctatc ccaccacaac aattgaagga catcttgtct 120

tctgttactt ggttcgaata cgaaagaggt agattgtctc aagaagcttg ttacgaaaga 180

tgtgctgaag aattcaagat cgaagcttct gttatcgctg aagctttcaa gcaagctaga 240

ggttctttga gaccaaacga agaattcatc gctttgatca gagacttgag aagagaaatg 300

cacggtgact tgactgtttt ggctttgtct aacatctctt tgccagacta cgaatacatc 360

atgtctttgt cttctgactg gactactgtt ttcgacagag ttttcccatc tgctttggtt 420

ggtgaaagaa agccacactt gggttgttac agaaaggtta tctctgaaat gaacttggaa 480

ccacaaacta ctgttttcgt tgacgacaag ttggacaacg ttgcttctgc tagatctttg 540

ggtatgcacg gtatcgtttt cgacaaccaa gctaacgttt tcagacaatt gagaaacatc 600

ttcggtgacc caatcagaag aggtcaagaa tacttgagag gtcacgctgg taagttggaa 660

tcttctactg acaacggttt gatcttcgaa gaaaacttca ctcaattgat catctacgaa 720

ttgactcaag acagaacttt gatctctttg tctgaatgtc caagaacttg gaacttcttc 780

agaggtgaac cattgttctc tgaaactttc ccagacgacg ttgacactac ttctgttgct 840

ttgactgttt tgcaaccaga cagagctttg gttaactctg ttttggacga aatgttggaa 900

tacgttgacg ctgacggtat catgcaaact tacttcgaca gatctagacc aagaatggac 960

ccattcgttt gtgttaacgt tttgtctttg ttctacgaaa acggtagagg tcacgaattg 1020

ccaagaactt tggactgggt ttacgaagtt ttgttgcaca gagcttacca cggtggttct 1080

agatactact tgtctccaga ctgtttcttg ttcttcatgt ctagattgtt gaagagagct 1140

gacgacccag ctgttcaagc tagattgaga ccattgttcg ttgaaagagt taacgaaaga 1200

gttggtgctg ctggtgactc tatggacttg gctttcagaa tcttggctgc tgcttctgtt 1260

ggtgttcaat gtccaagaga cttggaaaga ttgactgctg gtcaatgtga cgacggtggt 1320

tgggacttgt gttggttcta cgttttcggt tctactggtg ttaaggctgg taacagaggt 1380

ttgactactg ctttggctgt tactgctatc caaactgcta tcggtagacc accatctcca 1440

tctccatctg ctgcttcttc ttctttcaga ccatcttctc catacaagtt cttgggtatc 1500

tctagaccag cttctccaat cagattcggt gacttgttga gaccatggag aaagatgtct 1560

agatctaact tgaagtctca ataa 1584

<210> 94

<211> 1623

<212> DNA

<213> XP_006461126 针对酿酒酵母表达而优化的cDNA

<400> 94

atggctccac cacaaagacc attcactgct atcgttttcg acatcggtga cgttttgttc 60

caatggtctg ctactactaa gacttctatc tctccaaaga ctttgagatc tatcttgaac 120

tgtccaactt ggttcgacta cgaaagaggt agattggctg aaaacgcttg ttacgctgct 180

atctctcaag aattcaacgt taacccagac gaagttagag acgctttctc tcaagctaga 240

gactctttgc aagctaacca cgacttcatc tctttgatca gagaattgaa ggctcaagct 300

aacggtagat tgagagttta cgctatgtct aacatctctt tgccagactg ggaagttttg 360

agaatgaagc cagctgactg ggacatcttc gaccacgttt tcacttctgg tgctgttggt 420

gaaagaaagc caaacttggc tttctacaga cacgttatcg ctgctactga cttgcaacca 480

caccaaacta tcttcgttga cgacaagttg gaaaacgttt tgtctgctag atctttgggt 540

ttcactggta tcgttttcga cgaaccatct gaagttaaga gagctttgag aaacttgatc 600

ggtgacccag ttcaaagagg tggtgaattc ttggttagaa acgctggtaa gttgggttct 660

atcactagaa ctactgctaa gcacgaatct atcccattgg acgaaaactt cgctcaattg 720

ttgatcttgg aaatcactgg taacagagct ttggttaact tggttgaaca cccacaaact 780

tggaacttct tccaaggtaa gggtcaattg actactgaag aattcccatt cgacttggac 840

actacttctt tgggtttgac tatcttgaag agatctagag aaatcgctga ctctgttatg 900

gacgaaatgt tggaatacgt tgacccagac ggtatcatcc aaacttactt cgaccacaga 960

agaccaagat tcgacccagt tgtttgtgtt aacgctttgt ctttgttcta cgcttacggt 1020

agaggtgaac aattgagatc tactttgact tgggttcacg aagttttgtt gaacagagct 1080

tacttggacg gtactagata ctacgaaact gctgaatgtt tcttgtactt catgtctaga 1140

ttgttggcta cttctggtga cccagacttg cactctttgt tgaagccatt gttgaaggaa 1200

agagttcaag aaagaatcgg tgctgacggt gactctttgg ctttggctat gagaatcttg 1260

gcttgtgact tcgttggtat cagagacgaa gttgacttga gaactttgtt gactttgcaa 1320

tgtgaagacg gtggttggga agttggttgg atgtacaagt acggttcttc tggtatctct 1380

atcggtaaca gaggtttggc tactgctttg gctatcaagg ctgttgacac tatgttccaa 1440

ccacaaatca gattctctga atctccaact gacactttgg ttgaaaacgc tatccacaag 1500

agaagaccat ctttctctga aaagttcttg ggtaagagac caagatctgg ttctttcaga 1560

aagccattgc aatggatctt gcaaggttct aagttgagaa agtctgttga aatcggttct 1620

taa 1623

<210> 95

<211> 1566

<212> DNA

<213> SEQ ID NO: 68 - LoTps1 针对酿酒酵母表达而优化的cDNA

<400> 95

atgtacactg ctttgatctt ggacttgggt gacgttttgt tctcttggtc ttctactact 60

aacactacta tcccaccaag acaattgaag gaaatcttgt cttctccagc ttggttcgaa 120

tacgaaagag gtagaatcac tcaagctgaa tgttacgaaa gagtttctgc tgaattctct 180

ttggacgcta ctgctgttgc tgaagctttc agacaagcta gagactcttt gagaccaaac 240

gacaagttct tgactttgat cagagaattg agacaacaat ctcacggtga attgactgtt 300

ttggctttgt ctaacatctc tttgccagac tacgaattca tcatggcttt ggactctaag 360

tggacttctg ttttcgacag agttttccca tctgctttgg ttggtgaaag aaagccacac 420

ttgggtgctt tcagacaagt tttgtctgaa atgaacttgg acccacacac tactgttttc 480

gttgacgaca agttggacaa cgttgtttct gctagatctt tgggtatgca cggtgttgtt 540

ttcgactctc aagacaacgt tttcagaatg ttgagaaaca tcttcggtga cccaatccac 600

agaggtagag actacttgag acaacacgct ggtagattgg aaacttctac tgacgctggt 660

gttgttttcg aagaaaactt cactcaattg atcatctacg aattgactaa cgacaagtct 720

ttgatcacta cttctaactg tgctagaact tggaacttct tcagaggtaa gccattgttc 780

tctgcttctt tcccagacga catggacact acttctgttg ctttgactgt tttgagattg 840

gaccacgctt tggttaactc tgttttggac gaaatgttga agtacgttga cgctgacggt 900

atcatgcaaa cttacttcga ccacactaga ccaagaatgg acccattcgt ttgtgttaac 960

gttttgtctt tgttccacga acaaggtaga ggtcacgaat tgccaaacac tttggaatgg 1020

gttcacgaag ttttgttgca cagagcttac atcggtggtt ctagatacta cttgtctgct 1080

gactgtttct tgttcttcat gtctagattg ttgcaaagaa tcactgaccc atctgttttg 1140

ggtagattca gaccattgtt catcgaaaga gttagagaaa gagttggtgc tactggtgac 1200

tctatcgact tggctttcag aatcatcgct gcttctactg ttggtatcca atgtccaaga 1260

gacttggaat ctttgttggc tgctcaatgt gaagacggtg gttgggactt gtgttggttc 1320

taccaatacg gttctactgg tgttaaggct ggtaacagag gtttgactac tgctttggct 1380

atcaaggcta tcgactctgc tatcgctaga ccaccatctc cagctttgtc tgttgcttct 1440

tcttctaagt ctgaaatccc aaagccaatc caaagatctt tgagaccatt gtctccaaga 1500

agattcggtg gtttcttgat gccatggaga agatctcaaa gaaacggtgt tgctgtttct 1560

tcttaa 1566

<210> 96

<211> 1602

<212> DNA

<213> EMD37666.1 针对酿酒酵母表达而优化的cDNA

<400> 96

atgtctgctg ctgctcaata cactactttg atcttggact tgggtgacgt tttgttcact 60

tggtctccaa agactaagac ttctatccca ccaagaactt tgaaggaaat cttgaactct 120

gctacttggt acgaatacga aagaggtaga atctctcaag acgaatgtta cgaaagagtt 180

ggtactgaat tcggtatcgc tccatctgaa atcgacaacg ctttcaagca agctagagac 240

tctatggaat ctaacgacga attgatcgct ttggttagag aattgaagac tcaattggac 300

ggtgaattgt tggttttcgc tttgtctaac atctctttgc cagactacga atacgttttg 360

actaagccag ctgactggtc tatcttcgac aaggttttcc catctgcttt ggttggtgaa 420

agaaagccac acttgggtgt ttacaagcac gttatcgctg aaactggtat cgacccaaga 480

actactgttt tcgttgacga caagatcgac aacgttttgt ctgctagatc tgttggtatg 540

cacggtatcg ttttcgaaaa gcaagaagac gttatgagag ctttgagaaa catcttcggt 600

gacccagtta gaagaggtag agaatacttg agaagaaacg ctatgagatt ggaatctgtt 660

actgaccacg gtgttgcttt cggtgaaaac ttcactcaat tgttgatctt ggaattgact 720

aacgacccat ctttggttac tttgccagac agaccaagaa cttggaactt cttcagaggt 780

aacggtggta gaccatctaa gccattgttc tctgaagctt tcccagacga cttggacact 840

acttctttgg ctttgactgt tttgcaaaga gacccaggtg ttatctcttc tgttatggac 900

gaaatgttga actacagaga cccagacggt atcatgcaaa cttacttcga cgacggtaga 960

caaagattgg acccattcgt taacgttaac gttttgactt tcttctacac taacggtaga 1020

ggtcacgaat tggaccaatg tttgacttgg gttagagaag ttttgttgta cagagcttac 1080

ttgggtggtt ctagatacta cccatctgct gactgtttct tgtacttcat ctctagattg 1140

ttcgcttgta ctaacgaccc agttttgcac caccaattga agccattgtt cgttgaaaga 1200

gttcaagaac aaatcggtgt tgaaggtgac gctttggaat tggctttcag attgttggtt 1260

tgtgcttctt tggacgttca aaacgctatc gacatgagaa gattgttgga aatgcaatgt 1320

gaagacggtg gttgggaagg tggtaacttg tacagattcg gtactactgg tttgaaggtt 1380

actaacagag gtttgactac tgctgctgct gttcaagcta tcgaagcttc tcaaagaaga 1440

ccaccatctc catctccatc tgttgaatct actaagtctc caatcactcc agttactcca 1500

atgttggaag ttccatcttt gggtttgtct atctctagac catcttctcc attgttgggt 1560

tacttcagat tgccatggaa gaagtctgct gaagttcact aa 1602

<210> 97

<211> 1461

<212> DNA

<213> XP_001217376.1 针对酿酒酵母表达而优化的cDNA

<400> 97

atggctatca ctaagggtcc agttaaggct ttgatcttgg acttctctaa cgttttgtgt 60

tcttggaagc caccatctaa cgttgctgtt ccaccacaaa tcttgaagat gatcatgtct 120

tctgacatct ggcacgacta cgaatgtggt agatactcta gagaagactg ttacgctaga 180

gttgctgaca gattccacat ctctgctgct gacatggaag acactttgaa gcaagctaga 240

aagtctttgc aagttcacca cgaaactttg ttgttcatcc aacaagttaa gaaggacgct 300

ggtggtgaat tgatggtttg tggtatgact aacactccaa gaccagaaca agacgttatg 360

cactctatca acgctgaata cccagttttc gacagaatct acatctctgg tttgatgggt 420

atgagaaagc catctatctg tttctaccaa agagttatgg aagaaatcgg tttgtctggt 480

gacgctatca tgttcatcga cgacaagttg gaaaacgtta tcgctgctca atctgttggt 540

atcagaggtg ttttgttcca atctcaacaa gacttgagaa gagttgtttt gaacttcttg 600

ggtgacccag ttcacagagg tttgcaattc ttggctgcta acgctaagaa gatggactct 660

gttactaaca ctggtgacac tatccaagac aacttcgctc aattgttgat cttggaattg 720

gctcaagaca gagaattggt taagttgcaa gctggtaaga gaacttggaa ctacttcatc 780

ggtccaccaa agttgactac tgctactttc ccagacgaca tggacactac ttctatggct 840

ttgtctgttt tgccagttgc tgaagacgtt gtttcttctg ttttggacga aatgttgaag 900

ttcgttactg acgacggtat cttcatgact tacttcgact cttctagacc aagagttgac 960

ccagttgttt gtatcaacgt tttgggtgtt ttctgtagac acaacagaga aagagacgtt 1020

ttgccaactt tccactggat cagagacatc ttgatcaaca gagcttactt gtctggtact 1080

agatactacc catctccaga cttgttcttg ttcttcttgg ctagattgtg tttggctgtt 1140

agaaaccaat ctttgagaga acaattggtt ttgccattgg ttgacagatt gagagaaaga 1200

gttggtgctc caggtgaagc tgtttctttg gctgctagaa tcttggcttg tagatctttc 1260

ggtatcgact ctgctagaga catggactct ttgagaggta agcaatgtga agacggtggt 1320

tggccagttg aatgggttta cagattcgct tctttcggtt tgaacgttgg taacagaggt 1380

ttggctactg ctttcgctgt tagagctttg gaatctccat acggtgaatc tgctgttaag 1440

gttatgagaa gaatcgttta a 1461

<210> 98

<211> 1680

<212> DNA

<213> 胡椒莓(Drimys lanceolata)_DlTps589 野生型DNA序列

<400> 98

atggatctta ttaatccctc cccagcggct tccaccctcc ctctcccagt tgatggagat 60

tcagaagttg ttaggcgatc tgccgggttt catccgacta tctggggcga tcacttcctc 120

tcctacaagc ccgatccaaa gaaaatagat gcatggaata aaagggttga agagctgaag 180

gaagaagtga agaagatatt aagcaatgca aaagggacgg tggaagagct gaatttgatt 240

gatgatctcg tacaccttgg gattagttat cattttgaga aggagattga tgatgctcta 300

caacacatct ttgataccca tcttgatgat tttcctaagg atgatctata tgtcgccgct 360

ctccgatttg gcgtcttaag gaaacagggg caccgtgttt ctccagatgt attcaaaaaa 420

ttcaaagatg agcaggggaa tttcaaggca gagttgagca ccgatgcgaa aggtttgcta 480

tgtttaaatg atgtggctta tctcagcaca agaggggaag atatcttgga tgaagccatt 540

cctttcactg aggagcacct taggtcttgt attagccatg tagattctca tatggcagca 600

aaaattgaac attctctcga gcttcccctt catcatcgca taccaaggct agagaacagg 660

cactacatct cagtctatga aggagacaag gaaaggaacg aagttgtcct tgagcttgcc 720

aatttagatt tcaatctgat tcaaatcttg caccaaagag agctgagaga catcacaatg 780

tggtggaagg agattgacct tgcagcaaag ctgcctttta ttagggatag gttggtggag 840

tgctactact ggatcatggg ggtctatttt gaaccaatat actcgagggc tagggttttt 900

tccaccaaaa tgacaatgtt ggtctcagtt gtggacgaca tatatgatgt gtatgctacc 960

gaggatgagc ttcaactatt cactgatgcc atctataggt gggatgctga tgacattgat 1020

cagctgcctc agtacttgaa agatgctttt atggtactct acaacactgt gaagactcta 1080

gaagaagaac ttgaaccaga aggaaactct tatcgtggat tctatgtaaa agatgcaatg 1140

aaggttttgg caagggatta ctttgtggag cacaaatggt ataacagaaa aattgtgcca 1200

tccgtagagg aatacttgaa aatttcttgc atcagtgtgg ccgttcatat ggctacagtt 1260

cactgtattg ctgggatgta tgaaattgca accaaagagg cattcgaatg gttgatgact 1320

gagcccaaac ttgttattga tgcatctctg attggtcgtc tccttgatga catgcagtcc 1380

acctcgtttg agcaacagag aggccacgtg tcatcagcag tacagtgtta catggctgaa 1440

tatggtgtaa cagcggaaga agcatgtgaa aagctccgag atatggctgc aattgcttgg 1500

aaagatgtga acgaggcatg ccttaggccc acggttttcc ctatgcctat ccttttgcct 1560

tctatcaact tggcacgtgt ggcagaagtc atctacctac gtggagatgg atacacgcac 1620

gctgggggtg agaccaagaa acacatcacg gccatgcttg ttaagccaat tgaagtctga 1680

<210> 99

<211> 559

<212> PRT

<213> 胡椒莓(Drimys lanceolata)_DlTps589蛋白

<400> 99

Met Asp Leu Ile Asn Pro Ser Pro Ala Ala Ser Thr Leu Pro Leu Pro

1 5 10 15

Val Asp Gly Asp Ser Glu Val Val Arg Arg Ser Ala Gly Phe His Pro

20 25 30

Thr Ile Trp Gly Asp His Phe Leu Ser Tyr Lys Pro Asp Pro Lys Lys

35 40 45

Ile Asp Ala Trp Asn Lys Arg Val Glu Glu Leu Lys Glu Glu Val Lys

50 55 60

Lys Ile Leu Ser Asn Ala Lys Gly Thr Val Glu Glu Leu Asn Leu Ile

65 70 75 80

Asp Asp Leu Val His Leu Gly Ile Ser Tyr His Phe Glu Lys Glu Ile

85 90 95

Asp Asp Ala Leu Gln His Ile Phe Asp Thr His Leu Asp Asp Phe Pro

100 105 110

Lys Asp Asp Leu Tyr Val Ala Ala Leu Arg Phe Gly Val Leu Arg Lys

115 120 125

Gln Gly His Arg Val Ser Pro Asp Val Phe Lys Lys Phe Lys Asp Glu

130 135 140

Gln Gly Asn Phe Lys Ala Glu Leu Ser Thr Asp Ala Lys Gly Leu Leu

145 150 155 160

Cys Leu Asn Asp Val Ala Tyr Leu Ser Thr Arg Gly Glu Asp Ile Leu

165 170 175

Asp Glu Ala Ile Pro Phe Thr Glu Glu His Leu Arg Ser Cys Ile Ser

180 185 190

His Val Asp Ser His Met Ala Ala Lys Ile Glu His Ser Leu Glu Leu

195 200 205

Pro Leu His His Arg Ile Pro Arg Leu Glu Asn Arg His Tyr Ile Ser

210 215 220

Val Tyr Glu Gly Asp Lys Glu Arg Asn Glu Val Val Leu Glu Leu Ala

225 230 235 240

Asn Leu Asp Phe Asn Leu Ile Gln Ile Leu His Gln Arg Glu Leu Arg

245 250 255

Asp Ile Thr Met Trp Trp Lys Glu Ile Asp Leu Ala Ala Lys Leu Pro

260 265 270

Phe Ile Arg Asp Arg Leu Val Glu Cys Tyr Tyr Trp Ile Met Gly Val

275 280 285

Tyr Phe Glu Pro Ile Tyr Ser Arg Ala Arg Val Phe Ser Thr Lys Met

290 295 300

Thr Met Leu Val Ser Val Val Asp Asp Ile Tyr Asp Val Tyr Ala Thr

305 310 315 320

Glu Asp Glu Leu Gln Leu Phe Thr Asp Ala Ile Tyr Arg Trp Asp Ala

325 330 335

Asp Asp Ile Asp Gln Leu Pro Gln Tyr Leu Lys Asp Ala Phe Met Val

340 345 350

Leu Tyr Asn Thr Val Lys Thr Leu Glu Glu Glu Leu Glu Pro Glu Gly

355 360 365

Asn Ser Tyr Arg Gly Phe Tyr Val Lys Asp Ala Met Lys Val Leu Ala

370 375 380

Arg Asp Tyr Phe Val Glu His Lys Trp Tyr Asn Arg Lys Ile Val Pro

385 390 395 400

Ser Val Glu Glu Tyr Leu Lys Ile Ser Cys Ile Ser Val Ala Val His

405 410 415

Met Ala Thr Val His Cys Ile Ala Gly Met Tyr Glu Ile Ala Thr Lys

420 425 430

Glu Ala Phe Glu Trp Leu Met Thr Glu Pro Lys Leu Val Ile Asp Ala

435 440 445

Ser Leu Ile Gly Arg Leu Leu Asp Asp Met Gln Ser Thr Ser Phe Glu

450 455 460

Gln Gln Arg Gly His Val Ser Ser Ala Val Gln Cys Tyr Met Ala Glu

465 470 475 480

Tyr Gly Val Thr Ala Glu Glu Ala Cys Glu Lys Leu Arg Asp Met Ala

485 490 495

Ala Ile Ala Trp Lys Asp Val Asn Glu Ala Cys Leu Arg Pro Thr Val

500 505 510

Phe Pro Met Pro Ile Leu Leu Pro Ser Ile Asn Leu Ala Arg Val Ala

515 520 525

Glu Val Ile Tyr Leu Arg Gly Asp Gly Tyr Thr His Ala Gly Gly Glu

530 535 540

Thr Lys Lys His Ile Thr Ala Met Leu Val Lys Pro Ile Glu Val

545 550 555

<210> 100

<211> 1680

<212> DNA

<213> 人工序列_来自胡椒莓(D. lanceolata)的DlTps589的密码子优化的DNA序列

<220>

<223> 来自胡椒莓(D. lanceolata)的DlTps589的密码子优化的DNA序列

<400> 100

atggacctga ttaacccgag ccctgctgca tccaccctgc cactgccagt cgatggtgat 60

agcgaagttg tgcgccgtag cgcgggtttc catccgacca tctggggtga ccactttctg 120

tcttataagc cggacccgaa aaagattgat gcgtggaaca agcgtgttga ggaactgaaa 180

gaagaggtca aaaagatttt gagcaatgcg aaaggcacgg ttgaggaact gaatttgatt 240

gacgacctgg tacacctggg tattagctat cactttgaga aagaaatcga cgacgcgctg 300

cagcatatct tcgatacgca cctggatgat ttcccgaaag atgacctcta cgtggctgcg 360

ctgcgttttg gcgtcctgcg taagcaaggc catcgtgtca gcccggacgt ctttaagaaa 420

ttcaaagacg agcaaggcaa cttcaaagcg gagctgtcaa ccgatgcaaa gggcctgttg 480

tgcctgaacg atgtggcgta cctgagcacc cgtggtgagg atatcctgga cgaagcgatc 540

ccgttcacgg aagaacattt gcgctcgtgc attagccacg ttgatagcca catggcagcg 600

aagattgagc actctctgga gctgccgctg caccatcgca ttccgcgttt agagaatcgc 660

cattacatct ccgtgtacga gggtgacaaa gagcgtaatg aagtcgttct ggagttggct 720

aacttggact ttaatcttat ccagatcctg caccagcgcg agctgcgcga catcacgatg 780

tggtggaaag aaattgatct ggccgcaaag ctgccgttta ttcgtgaccg tctggtggag 840

tgttactatt ggattatggg cgtgtacttc gagccgatct acagccgtgc gcgcgtgttt 900

agcaccaaga tgaccatgct ggttagcgtg gtggatgaca tctatgatgt ctacgctacg 960

gaagatgagt tgcagctgtt taccgacgcc atttacagat gggacgccga tgacattgat 1020

caactgccgc aatatctgaa agacgccttt atggttctgt acaacaccgt caaaaccctg 1080

gaagaagaac tggagccgga aggtaactct tatcgtggtt tctacgttaa agatgcgatg 1140

aaagttctgg cgcgtgacta tttcgttgag cataagtggt acaatcgtaa gatcgtcccg 1200

tccgttgaag agtacttgaa gattagctgt atcagcgtcg cagtccacat ggcgaccgtg 1260

cactgtatcg ccggcatgta tgagatcgcc acgaaagaag cattcgagtg gctgatgacc 1320

gagccgaaac tggtgattga cgcaagcctg attggtcgcc tgctggacga tatgcagagc 1380

acgagctttg agcagcagcg cggtcatgtt agctccgcag ttcaatgcta catggctgag 1440

tacggtgtga ctgccgaaga agcatgcgag aagctgcgtg atatggcggc cattgcgtgg 1500

aaagatgtga atgaagcatg cctgcgcccg accgttttcc cgatgccgat tttactgcct 1560

agcatcaacc tggcacgtgt ggcggaagtt atctatctgc gtggcgacgg ttatacgcac 1620

gcgggtggtg agactaagaa gcacatcacc gcgatgctgg tcaagccgat cgaagtgtaa 1680

<210> 101

<211> 1656

<212> DNA

<213> 辛酸八角木(Drimys winteri)_SCH51_3228_9 野生型DNA序列

<400> 101

atggcttcca ccctccctct cccagcttat ggagattcag aagttgttag gcgatctgcc 60

gggtttcatc cgacgatctg gggcgatcac ttcctctcct acaagcctga tccaacgaaa 120

atagatgaat ggaataaaag ggttgaagag ctgaaggaag aagtgaagaa gatattaagc 180

aatgcaaaag ggacagtgga agagctgaat ttgcttgatg atctcgtaca ccttgggatt 240

agttatcatt ttgagaagga gattgatgat gctttacaac aaatctttga tacccatctt 300

gatgtttttc ctaaggatga tctatatgcc accgctctcc gatttggcgt cttaaggaaa 360

caggggcacc gtgtttctcc agatgtattc aaaaaattca aagatgagca ggggaatttc 420

aaggcagagt tgagcaccga tgcgaagggt ttgctatgtt tatatgatgt ggcttatctc 480

agcacaagag gggaagatat cttggatgaa gccattcctt tcactaagga gcaccttagg 540

tcttgtatta gccatgtcga ttctcatatg gcagcaaaaa ttgagcattc tctagagctt 600

ccccttcatc atcgcatacc aaggctagag aacaggcact acatctcagt ctatgaagga 660

gacaaggaaa ggaatgaagt tgtccttgag cttgccaaat tagatttcaa tctgattcaa 720

atcttgcacc aaagagagct gagggacatc acaacgtggt ggaaggagat tgaccttgca 780

gcaaagctac cttttattag ggataggttg gtggagtgct actattggat catgggagtc 840

tattttgaac caatatactc aagggctaga gttttttcga ccaaaatgac aatcttggtc 900

tcagttgtgg acgacatata tgatgtatat gctacagagg atgagctcca acttttcact 960

gatgcaatct ataggtggga tgctgaggac attgagcagc ttccacagta cttgaaagat 1020

gcttttcttg tactctataa cactgtgaag gacctagaag aggaattgga accagaagga 1080

aactcttatc gtggatacta tgtaaaagat gcgatgaagg ttttggcaag ggattacttt 1140

gtggagcaca aatggtataa cagaaaaatt gtgccatcag tagaggacta cctgcgaatt 1200

tcttgcatta gtgttgccgt tcatatggcc acagttcatt gtattgctgg gatgtatgaa 1260

attgcaacca aagaggcatt cgaatggttg aagacggaac ctaaacttgt tatagatgca 1320

tcactgattg ggcgtctcct cgatgacatg cagtccacct cgtttgagca acagagaggt 1380

catgtgtcat cagcggtaca gtgttacatg atccaatatg gggtatcaca cgaagaagcg 1440

tgtgagaagt tgcgagaaat ggctgcaatt gcgtggaaag atgtaaacca agcatgcctt 1500

aggcccactg ttttccctat gcctattctt ctgccctcca tcaaccttgc acgtgtggca 1560

gaagtgattt acctacgcgg agatggatat acacatgcgg gtggtgagac caaaaaacat 1620

atcacggcca tgcttgttga tccaatcaaa gtctga 1656

<210> 102

<211> 551

<212> PRT

<213> 辛酸八角木(Drimys winteri)_SCH51_3228_9蛋白

<400> 102

Met Ala Ser Thr Leu Pro Leu Pro Ala Tyr Gly Asp Ser Glu Val Val

1 5 10 15

Arg Arg Ser Ala Gly Phe His Pro Thr Ile Trp Gly Asp His Phe Leu

20 25 30

Ser Tyr Lys Pro Asp Pro Thr Lys Ile Asp Glu Trp Asn Lys Arg Val

35 40 45

Glu Glu Leu Lys Glu Glu Val Lys Lys Ile Leu Ser Asn Ala Lys Gly

50 55 60

Thr Val Glu Glu Leu Asn Leu Leu Asp Asp Leu Val His Leu Gly Ile

65 70 75 80

Ser Tyr His Phe Glu Lys Glu Ile Asp Asp Ala Leu Gln Gln Ile Phe

85 90 95

Asp Thr His Leu Asp Val Phe Pro Lys Asp Asp Leu Tyr Ala Thr Ala

100 105 110

Leu Arg Phe Gly Val Leu Arg Lys Gln Gly His Arg Val Ser Pro Asp

115 120 125

Val Phe Lys Lys Phe Lys Asp Glu Gln Gly Asn Phe Lys Ala Glu Leu

130 135 140

Ser Thr Asp Ala Lys Gly Leu Leu Cys Leu Tyr Asp Val Ala Tyr Leu

145 150 155 160

Ser Thr Arg Gly Glu Asp Ile Leu Asp Glu Ala Ile Pro Phe Thr Lys

165 170 175

Glu His Leu Arg Ser Cys Ile Ser His Val Asp Ser His Met Ala Ala

180 185 190

Lys Ile Glu His Ser Leu Glu Leu Pro Leu His His Arg Ile Pro Arg

195 200 205

Leu Glu Asn Arg His Tyr Ile Ser Val Tyr Glu Gly Asp Lys Glu Arg

210 215 220

Asn Glu Val Val Leu Glu Leu Ala Lys Leu Asp Phe Asn Leu Ile Gln

225 230 235 240

Ile Leu His Gln Arg Glu Leu Arg Asp Ile Thr Thr Trp Trp Lys Glu

245 250 255

Ile Asp Leu Ala Ala Lys Leu Pro Phe Ile Arg Asp Arg Leu Val Glu

260 265 270

Cys Tyr Tyr Trp Ile Met Gly Val Tyr Phe Glu Pro Ile Tyr Ser Arg

275 280 285

Ala Arg Val Phe Ser Thr Lys Met Thr Ile Leu Val Ser Val Val Asp

290 295 300

Asp Ile Tyr Asp Val Tyr Ala Thr Glu Asp Glu Leu Gln Leu Phe Thr

305 310 315 320

Asp Ala Ile Tyr Arg Trp Asp Ala Glu Asp Ile Glu Gln Leu Pro Gln

325 330 335

Tyr Leu Lys Asp Ala Phe Leu Val Leu Tyr Asn Thr Val Lys Asp Leu

340 345 350

Glu Glu Glu Leu Glu Pro Glu Gly Asn Ser Tyr Arg Gly Tyr Tyr Val

355 360 365

Lys Asp Ala Met Lys Val Leu Ala Arg Asp Tyr Phe Val Glu His Lys

370 375 380

Trp Tyr Asn Arg Lys Ile Val Pro Ser Val Glu Asp Tyr Leu Arg Ile

385 390 395 400

Ser Cys Ile Ser Val Ala Val His Met Ala Thr Val His Cys Cys Ala

405 410 415

Gly Met Asp Glu Ile Ala Thr Lys Glu Ala Phe Glu Trp Leu Lys Thr

420 425 430

Glu Pro Lys Leu Val Ile Asp Ala Ser Leu Ile Gly Arg Leu Leu Asp

435 440 445

Asp Met Gln Ser Thr Ser Phe Glu Gln Gln Arg Gly His Val Ser Ser

450 455 460

Ala Val Gln Cys Tyr Met Ile Gln Tyr Gly Val Ser His Glu Glu Ala

465 470 475 480

Cys Glu Lys Leu Arg Glu Met Ala Ala Ile Ala Trp Lys Asp Val Asn

485 490 495

Gln Ala Cys Leu Arg Pro Thr Val Phe Pro Met Pro Ile Leu Leu Pro

500 505 510

Ser Ile Asn Leu Ala Arg Val Ala Glu Val Ile Tyr Leu Arg Gly Asp

515 520 525

Gly Tyr Thr His Ala Gly Gly Glu Thr Lys Lys His Ile Thr Ala Met

530 535 540

Leu Val Asp Pro Ile Lys Val

545 550

<210> 103

<211> 1656

<212> DNA

<213> 人工序列_SCH51-3228-9的密码子优化的DNA序列

<220>

<223> SCH51-3228-9的密码子优化的DNA序列

<400> 103

atggcaagca ccctgccgct gcctgcctat ggtgatagcg aagttgttcg tcgtagcgca 60

ggttttcatc cgaccatttg gggtgatcat tttctgagct ataaaccgga tccgaccaaa 120

attgatgaat ggaataaacg tgtcgaagaa ctgaaagaag aagtgaaaaa aatcctgagc 180

aatgccaaag gcaccgttga ggaactgaat ctgctggatg atctggttca tctgggtatc 240

agctatcact ttgagaaaga aatcgatgat gcactgcagc agatttttga tacccatctg 300

gatgttttcc cgaaagatga tctgtatgca accgcactgc gttttggtgt tctgcgtaaa 360

cagggtcatc gtgttagtcc ggatgtgttc aaaaaattca aagatgaaca gggcaacttc 420

aaagcagaac tgagcaccga tgcaaaaggt ctgctgtgtc tgtatgatgt tgcatatctg 480

agcacccgtg gtgaagatat tctggatgaa gcaattccgt ttaccaaaga acatctgcgt 540

agctgtatta gccatgttga tagccacatg gcagcgaaaa ttgaacatag cctggaactg 600

cctctgcatc accgtattcc gcgtctggaa aatcgtcact atattagcgt ttatgagggc 660

gataaagaac gcaatgaagt tgtgctggaa ctggcaaaac tggattttaa cctgattcag 720

attctgcatc agcgtgaact gcgtgatatt accacctggt ggaaagaaat tgatctggca 780

gcaaaactgc cgtttattcg tgatcgtctg gttgaatgct attattggat tatgggcgtg 840

tatttcgaac cgatttatag ccgtgcacgt gtttttagca ccaaaatgac cattctggtt 900

agcgtggtgg atgatatcta tgatgtttat gccaccgaag atgaactgca gctgtttacc 960

gatgccattt atcgttggga tgcagaagat attgaacagc tgccgcagta tctgaaagat 1020

gcatttctgg ttctgtacaa caccgtgaaa gatctggaag aagaactgga accggaaggt 1080

aatagctatc gtggttatta tgttaaagat gccatgaaag ttctggcacg cgattatttt 1140

gttgagcaca aatggtataa ccgcaaaatt gttccgagcg tggaagatta tctgcgtatt 1200

agctgcatta gcgttgcagt tcacatggca accgttcatt gttgtgcagg tatggatgaa 1260

attgcaacca aagaagcatt tgagtggctg aaaaccgaac cgaaactggt tattgatgca 1320

agcctgattg gtcgtctgct ggacgatatg cagagcacca gctttgaaca gcagcgtggt 1380

catgttagca gcgcagttca gtgttatatg attcagtatg gtgttagcca tgaagaagca 1440

tgcgaaaaac tgcgcgaaat ggcagcaatt gcatggaaag atgttaatca ggcatgtctg 1500

cgtccgaccg tttttccgat gccgattctg ctgccgagca ttaatctggc acgtgttgcc 1560

gaagttatct atctgcgtgg tgatggttat acccatgccg gtggtgaaac caaaaaacat 1620

attaccgcaa tgctggtcga tccgattaaa gtttaa 1656

<210> 104

<211> 1656

<212> DNA

<213> 辛酸八角木(Drimys winteri)_SCH51_3228_11 野生型DNA序列

<400> 104

atggcttcca ccctccctct cccagcttat ggagattcag aagttgttag gcgatctgcc 60

gggtttcatc cgacgatctg gggcgatcac ttcctctcct acaagcctga tccaacgaaa 120

atagatgaat ggaataaaag ggttgaagag ctgaaggaag aagtgaagaa gatattaagc 180

aatgcaaaag ggacagtgga agagctgaat ttgcttgatg atctcgtaca ccttgggatt 240

agttatcatt ttgagaagga gattgatgat gctttacaac aaatctttga tacccatctt 300

gatgtttttc ctaaggatga tctatatgcc accgctctcc gatttggcgt cttaaggaaa 360

caggggcacc gtgtttctcc agatgtattc aaaaaattca aagatgagca ggggaatttc 420

aaggcagagt tgagcaccga tgcgaagggt ttgctatgtt tatatgatgt ggcttatctc 480

agcacaagag gggaagatat cttggatgaa gccattcctt tcactaagga gcaccttagg 540

tcttgtatta gccatgtcga ttctcatatg gcagcaaaaa ttgagcattc tctagagctt 600

ccccttcatc atcgcatacc aaggctagag aacaggcact acatctcagt ctatgaagga 660

gacaaggaaa ggaatgaagt tgtccttgag cttgccaaat tagatttcaa tctgattcaa 720

atcttgcacc aaagagagct gagggacatc acaatgtggt ggaaggagat tgaccttgca 780

gcaaagctac cttttattag agataggttg gtggagtgct actactggat catgggggtc 840

tattttgaac caatatactc cagggctagg gttttttcca ctaaaatgac aatcttggtc 900

tcagttgtgg acgacatata tgatgtctat gctacggagg atgagcttca actattcact 960

gatgcaatct ataggtggga tgctgatgac attgatcagc tgcctcagta cttgaaagat 1020

gcttttatgg tactctataa cactgtgaag actctagaag aagaacttga accagaagga 1080

aactcttatc gtggatacta cgtaaaagat gcaatgaagg ttttggcaag agattacttt 1140

gtggaacaca aatggtataa cagacaaatt gtgccatccg tagaggaata cttgaaaatt 1200

tcttgcatta gtgtggctgt tcatatggct acagttcatt gtattgctgg gatgtatgaa 1260

attgctacca aagaggcatt cgaatggttg aagactgaac ccaaacttgt tatcgatgca 1320

tctctgatcg gtcgtcttct tgatgacatg cagtctacct cgtttgagca acaaagaggg 1380

cacgtgtcat cagcagtaca gtgttacatg gcccaatatg gagtaacagc agaagaagca 1440

tgtgaaaagc tacgagaaat ggctgcaatt gcttggaaag atgtgaatga agcatgcctt 1500

aggcccacgg tattccctat gcctatcctc ttgccttcta tcaacttggc acgtgtggca 1560

gaagtgatct acctacgtgg agatggatac acgcacgctg ggggtgagac caaaaaacac 1620

atcacggcca tgcttgttaa gccaattgaa gtctga 1656

<210> 105

<211> 551

<212> PRT

<213> 辛酸八角木(Drimys winteri)_SCH51_3228_11蛋白

<400> 105

Met Ala Ser Thr Leu Pro Leu Pro Ala Tyr Gly Asp Ser Glu Val Val

1 5 10 15

Arg Arg Ser Ala Gly Phe His Pro Thr Ile Trp Gly Asp His Phe Leu

20 25 30

Ser Tyr Lys Pro Asp Pro Thr Lys Ile Asp Glu Trp Asn Lys Arg Val

35 40 45

Glu Glu Leu Lys Glu Glu Val Lys Lys Ile Leu Ser Asn Ala Lys Gly

50 55 60

Thr Val Glu Glu Leu Asn Leu Leu Asp Asp Leu Val His Leu Gly Ile

65 70 75 80

Ser Tyr His Phe Glu Lys Glu Ile Asp Asp Ala Leu Gln Gln Ile Phe

85 90 95

Asp Thr His Leu Asp Val Phe Pro Lys Asp Asp Leu Tyr Ala Thr Ala

100 105 110

Leu Arg Phe Gly Val Leu Arg Lys Gln Gly His Arg Val Ser Pro Asp

115 120 125

Val Phe Lys Lys Phe Lys Asp Glu Gln Gly Asn Phe Lys Ala Glu Leu

130 135 140

Ser Thr Asp Ala Lys Gly Leu Leu Cys Leu Tyr Asp Val Ala Tyr Leu

145 150 155 160

Ser Thr Arg Gly Glu Asp Ile Leu Asp Glu Ala Ile Pro Phe Thr Lys

165 170 175

Glu His Leu Arg Ser Cys Ile Ser His Val Asp Ser His Met Ala Ala

180 185 190

Lys Ile Glu His Ser Leu Glu Leu Pro Leu His His Arg Ile Pro Arg

195 200 205

Leu Glu Asn Arg His Tyr Ile Ser Val Tyr Glu Gly Asp Lys Glu Arg

210 215 220

Asn Glu Val Val Leu Glu Leu Ala Lys Leu Asp Phe Asn Leu Ile Gln

225 230 235 240

Ile Leu His Gln Arg Glu Leu Arg Asp Ile Thr Met Trp Trp Lys Glu

245 250 255

Ile Asp Leu Ala Ala Lys Leu Pro Phe Ile Arg Asp Arg Leu Val Glu

260 265 270

Cys Tyr Tyr Trp Ile Met Gly Val Tyr Phe Glu Pro Ile Tyr Ser Arg

275 280 285

Ala Arg Val Phe Ser Thr Lys Met Thr Ile Leu Val Ser Val Val Asp

290 295 300

Asp Ile Tyr Asp Val Tyr Ala Thr Glu Asp Glu Leu Gln Leu Phe Thr

305 310 315 320

Asp Ala Ile Tyr Arg Trp Asp Ala Asp Asp Ile Asp Gln Leu Pro Gln

325 330 335

Tyr Leu Lys Asp Ala Phe Met Val Leu Tyr Asn Thr Val Lys Thr Leu

340 345 350

Glu Glu Glu Leu Glu Pro Glu Gly Asn Ser Tyr Arg Gly Tyr Tyr Val

355 360 365

Lys Asp Ala Met Lys Val Leu Ala Arg Asp Tyr Phe Val Glu His Lys

370 375 380

Trp Tyr Asn Arg Gln Ile Val Pro Ser Val Glu Glu Tyr Leu Lys Ile

385 390 395 400

Ser Cys Ile Ser Val Ala Val His Met Ala Thr Val His Cys Ile Ala

405 410 415

Gly Met Tyr Glu Ile Ala Thr Lys Glu Ala Phe Glu Trp Leu Lys Thr

420 425 430

Glu Pro Lys Leu Val Ile Asp Ala Ser Leu Ile Gly Arg Leu Leu Asp

435 440 445

Asp Met Gln Ser Thr Ser Phe Glu Gln Gln Arg Gly His Val Ser Ser

450 455 460

Ala Val Gln Cys Tyr Met Ala Gln Tyr Gly Val Thr Ala Glu Glu Ala

465 470 475 480

Cys Glu Lys Leu Arg Glu Met Ala Ala Ile Ala Trp Lys Asp Val Asn

485 490 495

Glu Ala Cys Leu Arg Pro Thr Val Phe Pro Met Pro Ile Leu Leu Pro

500 505 510

Ser Ile Asn Leu Ala Arg Val Ala Glu Val Ile Tyr Leu Arg Gly Asp

515 520 525

Gly Tyr Thr His Ala Gly Gly Glu Thr Lys Lys His Ile Thr Ala Met

530 535 540

Leu Val Lys Pro Ile Glu Val

545 550

<210> 106

<211> 1656

<212> DNA

<213> 人工序列_SCH51-3228-11的密码子优化的DNA序列

<220>

<223> SCH51-3228-11的密码子优化的DNA序列

<400> 106

atggcatcta ctcttccact gccggcttat ggtgattctg aggttgttcg tcgttccgcg 60

ggttttcacc ctaccatctg gggcgatcac tttctgtcct ataagccaga cccgaccaag 120

attgacgagt ggaataagcg tgtcgaggaa ctgaaagaag aagtgaaaaa gatcctgtcc 180

aacgcaaaag gtactgtcga ggagctgaat ctgctggatg acctggtgca tctgggcatc 240

agctatcact tcgaaaagga aattgacgac gctttgcagc aaatttttga tacgcacctg 300

gacgtctttc cgaaagatga cctgtatgcg accgcgctgc gctttggtgt gctgcgtaaa 360

cagggtcatc gcgtgtctcc tgatgtgttc aagaaattta aagatgaaca gggcaatttc 420

aaggccgagt tgagcacgga cgccaaaggt ttgctctgcc tgtacgacgt tgcatatctg 480

agcacccgtg gtgaagatat cctggacgaa gcgattccgt tcaccaagga acatctgcgc 540

tcgtgcattt cccatgtaga tagccacatg gcggccaaga tcgagcacag cctggagctg 600

cctttgcacc atcgtattcc gcgcctggag aatcgccatt acattagcgt ctatgagggt 660

gacaaagagc gcaacgaagt cgtgttagag ctggcgaagc tggacttcaa cctgattcaa 720

attctgcatc aacgcgagct gcgcgacatt accatgtggt ggaaagagat tgatctggca 780

gcgaagctgc cgttcatccg cgatcgtctg gttgagtgct actactggat catgggcgtc 840

tacttcgagc cgatctacag ccgcgctcgt gtgttttcga cgaagatgac catcctggtt 900

agcgttgttg atgacattta tgacgtttac gcgaccgaag atgaactgca gctgtttacg 960

gacgcaatct accgttggga cgcggatgat atcgaccagc tgccgcaata cttgaaagat 1020

gcgttcatgg ttttgtacaa caccgtcaaa acgctggaag aagaactgga gccggaaggc 1080

aacagctacc gtggttacta tgttaaagat gcgatgaaag ttctggcgcg cgactacttc 1140

gtcgagcaca agtggtataa ccgtcagatt gtgccgagcg tcgaggaata cctgaagatt 1200

agctgtatca gcgttgccgt tcacatggca acggtgcact gcatcgccgg tatgtacgag 1260

attgcgacga aagaagcctt cgaatggttg aaaaccgagc cgaagctggt tatcgacgcc 1320

agcctgatcg gtcgtttgct ggacgacatg caaagcacga gcttcgagca gcagcgcggc 1380

catgtgagca gcgctgttca gtgttatatg gcgcaatatg gcgtgaccgc agaagaagcg 1440

tgcgagaagc tgcgtgagat ggcagcaatt gcgtggaaag atgtgaatga agcctgtctg 1500

cgtccgactg tgtttccgat gccgatcctg ctgccgagca ttaacctggc gcgtgtggca 1560

gaggtcatct atctgcgtgg tgacggttac acccacgcgg gtggcgaaac caagaaacat 1620

atcaccgcaa tgctggttaa gccgattgaa gtgtaa 1656

<210> 107

<211> 1677

<212> DNA

<213> 辛酸八角木(Drimys winteri)_SCH51_998_28 野生型DNA序列

<400> 107

atggatctta gtacttcacc tgttctttct tcctcccccc ttccggtgga agacggaaaa 60

aatccggccg ttcgccgttc agctggattt caccccagta tttggggtga tcatttcctc 120

tcctacactg aagatcacaa gaagctggat gcatggagcg aaaggactca agtgttgaag 180

gaagaggtga ggagaatttt aatcaatgcc aaggggtcac tagaagagtt ggatttgttg 240

gatgcaatcc aacgccttgg ggtgaaatat cactttgaga aagagattga agaggcatta 300

caccatattt atgttgcaga aactcatgtt tctactgatg acttatattc cgtttctctc 360

cggtttcgac ttcttagaca acaagggtac aatgtatctg ctgatgtatt taaaaagttc 420

aaagatgaga ggggcaactt caaggcaagc ttaagtactg atgccagggg gttgctaagc 480

ttgtatgaag ctgcatttct cagcatacga ggagatgata tcttagatga agccataact 540

ttcacaagag agcagcttaa gtcttctatg acccatgttg atgcccctct tgccaaacaa 600

atagcccatg ccttagaggt accagcgcac aagcgcatac aaagactaga gaacattcgc 660

tacctcacaa tctaccaaga agagaaagga aggaatgatg tgttgcttga gcttgccaag 720

ttggatttca atatcttaca acaattgcat aagaaagaac tgagagacct tacaaagtgg 780

tggaaggaca cagacgttgc aggaaagcta cctttcatca gagataggtt ggtggaatgc 840

tattattgga tcttgggtgt gtattatgag ccagaatact ccagagctag aattttttct 900

accaaaatga caatcatggt ctcagttgtt gatgacatat atgacgtata tgctactgaa 960

gatgagctcc aactattcac tgatgcaatc tataggtggg atctggaggg cctagatcaa 1020

ctcccacagt tcttgaaaga ctgttttctt gtactctatg acaccgtcaa ggaattagaa 1080

gacgaactag aaccggaagg aaaatcctat cgtggatact atgtaaagga tgcgatgaag 1140

gttttggcta gagattactt cgttgagcac aaatggtata acagaaacat agtgccaagt 1200

gtagaagaat atctccgtgt ttcttgcatc agtgttgcag tccatatggc taacgtccat 1260

tgctgtgctg ggatgggaga tgtaatgagc aaagaggcat tcgaatggtt gaagagtgaa 1320

ccaaaggttg taatggatgc atcactaatt ggccgactgc tcgatgacat gcagtccacc 1380

gagtttgagc aaaagagagg ccatgttgca tcggctgtcc aatgttacat gaatgagtat 1440

ggagtgactt acaaagaagc gtgtgaaaag ctgcatgaaa tggctgccct tgcatggaaa 1500

gacgtaaacc aggcttgcct taaaccaact gttttccctc tccctgtatt tatgcctgca 1560

atcaaccttg cgcgagtggc tgaagtcatc taccttcgtg gagatgggta tactcattca 1620

ggaggagaga ctaaagaaaa tatcacgttg atgcttgtca atccaatctc tgtgtga 1677

<210> 108

<211> 558

<212> PRT

<213> 辛酸八角木(Drimys winteri)_SCH51_998_28蛋白

<400> 108

Met Asp Leu Ser Thr Ser Pro Val Leu Ser Ser Ser Pro Leu Pro Val

1 5 10 15

Glu Asp Gly Lys Asn Pro Ala Val Arg Arg Ser Ala Gly Phe His Pro

20 25 30

Ser Ile Trp Gly Asp His Phe Leu Ser Tyr Thr Glu Asp His Lys Lys

35 40 45

Leu Asp Ala Trp Ser Glu Arg Thr Gln Val Leu Lys Glu Glu Val Arg

50 55 60

Arg Ile Leu Ile Asn Ala Lys Gly Ser Leu Glu Glu Leu Asp Leu Leu

65 70 75 80

Asp Ala Ile Gln Arg Leu Gly Val Lys Tyr His Phe Glu Lys Glu Ile

85 90 95

Glu Glu Ala Leu His His Ile Tyr Val Ala Glu Thr His Val Ser Thr

100 105 110

Asp Asp Leu Tyr Ser Val Ser Leu Arg Phe Arg Leu Leu Arg Gln Gln

115 120 125

Gly Tyr Asn Val Ser Ala Asp Val Phe Lys Lys Phe Lys Asp Glu Arg

130 135 140

Gly Asn Phe Lys Ala Ser Leu Ser Thr Asp Ala Arg Gly Leu Leu Ser

145 150 155 160

Leu Tyr Glu Ala Ala Phe Leu Ser Ile Arg Gly Asp Asp Ile Leu Asp

165 170 175

Glu Ala Ile Thr Phe Thr Arg Glu Gln Leu Lys Ser Ser Met Thr His

180 185 190

Val Asp Ala Pro Leu Ala Lys Gln Ile Ala His Ala Leu Glu Val Pro

195 200 205

Ala His Lys Arg Ile Gln Arg Leu Glu Asn Ile Arg Tyr Leu Thr Ile

210 215 220

Tyr Gln Glu Glu Lys Gly Arg Asn Asp Val Leu Leu Glu Leu Ala Lys

225 230 235 240

Leu Asp Phe Asn Ile Leu Gln Gln Leu His Lys Lys Glu Leu Arg Asp

245 250 255

Leu Thr Lys Trp Trp Lys Asp Thr Asp Val Ala Gly Lys Leu Pro Phe

260 265 270

Ile Arg Asp Arg Leu Val Glu Cys Tyr Tyr Trp Ile Leu Gly Val Tyr

275 280 285

Tyr Glu Pro Glu Tyr Ser Arg Ala Arg Ile Phe Ser Thr Lys Met Thr

290 295 300

Ile Met Val Ser Val Val Asp Asp Ile Tyr Asp Val Tyr Ala Thr Glu

305 310 315 320

Asp Glu Leu Gln Leu Phe Thr Asp Ala Ile Tyr Arg Trp Asp Leu Glu

325 330 335

Gly Leu Asp Gln Leu Pro Gln Phe Leu Lys Asp Cys Phe Leu Val Leu

340 345 350

Tyr Asp Thr Val Lys Glu Leu Glu Asp Glu Leu Glu Pro Glu Gly Lys

355 360 365

Ser Tyr Arg Gly Tyr Tyr Val Lys Asp Ala Met Lys Val Leu Ala Arg

370 375 380

Asp Tyr Phe Val Glu His Lys Trp Tyr Asn Arg Asn Ile Val Pro Ser

385 390 395 400

Val Glu Glu Tyr Leu Arg Val Ser Cys Ile Ser Val Ala Val His Met

405 410 415

Ala Asn Val His Cys Cys Ala Gly Met Gly Asp Val Met Ser Lys Glu

420 425 430

Ala Phe Glu Trp Leu Lys Ser Glu Pro Lys Val Val Met Asp Ala Ser

435 440 445

Leu Ile Gly Arg Leu Leu Asp Asp Met Gln Ser Thr Glu Phe Glu Gln

450 455 460

Lys Arg Gly His Val Ala Ser Ala Val Gln Cys Tyr Met Asn Glu Tyr

465 470 475 480

Gly Val Thr Tyr Lys Glu Ala Cys Glu Lys Leu His Glu Met Ala Ala

485 490 495

Leu Ala Trp Lys Asp Val Asn Gln Ala Cys Leu Lys Pro Thr Val Phe

500 505 510

Pro Leu Pro Val Phe Met Pro Ala Ile Asn Leu Ala Arg Val Ala Glu

515 520 525

Val Ile Tyr Leu Arg Gly Asp Gly Tyr Thr His Ser Gly Gly Glu Thr

530 535 540

Lys Glu Asn Ile Thr Leu Met Leu Val Asn Pro Ile Ser Val

545 550 555

<210> 109

<211> 1677

<212> DNA

<213> 人工序列_SCH51-998-28的密码子优化的DNA序列

<220>

<223> SCH51-998-28的密码子优化的DNA序列

<400> 109

atggatctga gcaccagtcc ggttctgagc agctcaccgc tgccggttga agatggtaaa 60

aatccggcag ttcgtcgtag cgcaggtttt catccgagca tttggggtga tcattttctg 120

agctataccg aggatcacaa aaaactggat gcatggtcag aacgtaccca ggttctgaaa 180

gaagaagtgc gtcgtattct gattaatgca aaaggtagcc tggaagaact ggatctgctg 240

gatgcaattc agcgtctggg tgttaaatat cactttgaga aagaaatcga agaagccctg 300

catcatattt atgttgcaga aacccatgtg tcaaccgatg atctgtatag cgttagcctg 360

cgttttcgtc tgctgcgtca gcagggttat aatgttagcg cagatgtgtt caaaaaattc 420

aaagatgaac gcggtaactt caaagcaagc ctgagcaccg atgcacgtgg tctgctgagc 480

ctgtatgaag cagcatttct gagcattcgt ggtgatgata ttctggatga agcaattacc 540

tttacccgtg aacagctgaa aagcagcatg acccatgttg atgcaccgct ggcaaaacaa 600

attgcacatg cactggaagt tccggcacat aaacgtattc agcgcctgga aaatattcgc 660

tatctgacca tttaccaaga agagaaaggt cgtaacgatg ttctgctgga actggccaaa 720

ctggatttta acattctgca gcagctgcat aaaaaagaac tgcgtgatct gaccaaatgg 780

tggaaagata ccgatgttgc aggtaaactg ccgtttattc gtgatcgtct ggttgaatgc 840

tattattgga ttctgggcgt ttattatgag ccggaatata gccgtgcacg tatttttagc 900

accaaaatga ccattatggt tagcgtggtg gatgacatct atgatgttta tgcaaccgaa 960

gatgaactgc agctgtttac cgatgcaatt tatcgttggg atctggaagg tctggatcag 1020

ctgccgcagt tcctgaaaga ttgttttctg gttctgtatg ataccgtgaa agaactggaa 1080

gatgagctgg aaccggaagg taaaagctat cgtggttatt atgttaaaga tgccatgaaa 1140

gttctggcac gcgattattt tgttgagcac aaatggtata accgcaatat tgttccgagc 1200

gtggaagaat atctgcgtgt tagctgtatt agcgttgcag ttcacatggc aaatgttcat 1260

tgttgtgcag gtatgggtga tgtgatgagc aaagaagcat ttgaatggct gaaaagtgaa 1320

ccgaaagttg ttatggatgc cagcctgatt ggtcgcctgc tggacgatat gcagagcacc 1380

gaatttgaac agaaacgtgg tcatgttgca agcgcagttc agtgttatat gaatgaatat 1440

ggcgtgacct ataaagaggc atgcgaaaaa ctgcatgaaa tggcagcact ggcatggaaa 1500

gatgttaatc aggcatgtct gaaaccgacc gtttttccgc tgcctgtttt tatgcctgca 1560

attaatctgg cacgtgttgc cgaagttatt tacctgcgtg gggatggtta tacccatagc 1620

ggtggtgaaa ccaaagaaaa cattaccctg atgctggtta atccgattag cgtttaa 1677

<210> 110

<211> 1680

<212> DNA

<213> 胡椒莓(Drimys lanceolata)_SCH52_13163_6

<400> 110

atggatgttc taattccctc ccctgtggct tccactctcc ctctgcccga agatggaaac 60

ttggacgtcg ttcgcagatc cgccgggttt catccgacgg tctggggcga tcacttcctc 120

gcttactcgc ccgatccaac caaaatagat gcttggacta aaagagttga agagctgaag 180

caagaagtga agaggattct aagcaatgtg aaagggtcac tggaagagct gaacttgctt 240

gatgctatcc aacaccttgg gattggttat cattttgaga aagagattga tgatgcttta 300

caactaatct ttgattccca tattgatgct tttcctactg atgatctata tgtggctgcc 360

ctccgattta gcctactaag gcgacaaggg cactgtgttt cttcagatgt attcaaaaaa 420

ttcaaagatg agcaggggaa tttcaaggca gagctgagca ccgatgcgaa aggtttgctg 480

agtctctatg acgcggcgta tctcagtgta agaggggaag atatattgga tgaggccatt 540

cctttcacta gggagcacct taggacttgt attagccatg tagattctca tttggcagca 600

aaaattgagc attctctaga gcttcccctg catcatcgca taccaaggct agagaacagg 660

cactacatct cagtgtacga aggagagaag gaaaggaatg aagttgtact agagcttgcc 720

aaattagatt tcaatctgat tcaaatcttg caccaaagag agctgaggga catcacaacg 780

tggtggaatg agattgacct cgcagcaaag ctaccattta ttagggatag gttggtggag 840

tgctactatt ggatcatggg tgtctatttt gaaccaatat tctcaagggc tagagttttt 900

tcgaccaaaa tgacaatttt ggtctcagtt gtcgacgaca tatatgatgt ctacgctaca 960

gaggatgagc tccaactttt cactgacgca atctataggt gggatgccga ggacattgag 1020

cagcttccac agtacttgaa agattctttt cttgtactct ataacaccgt gaaggactta 1080

gaagaggagc tgaaaccaga aggaaactca tatcgtggag actatgtaaa agatgcgatg 1140

aaggttttgg caagagatta ctttgtggag cacaaatggt ataacagaaa aattgtaccg 1200

tcagtagagg actacctacg aatttcttgc attagtgttg ccgttcatat ggctacagtt 1260

cattgttgtg ctgggatgga tgaaattgca accaaagagg cattcgaatg gttgaagacc 1320

gaacctaaac ttgttataga tgcatcactg attgggcgtc tcctcgatga catgcagtcc 1380

acctcgtttg agcaacagag aggtcatgtg tcatcggcgg tacagtgtta catgatccaa 1440

tatggcgtat cacacgaaga agcgtgtgag aagttgacag aaatggctgc aattgcatgg 1500

aaagatgtaa accaagcatg ccttaggccc actgttttcc caatgcctat tcttctgcct 1560

tcaatcaacc ttgcacgtgt ggcagaagtc atctacctgc gcggagatgg atatacacat 1620

gctggtggtg agaccaaaaa acatatcacg gccatgcttg ttgaaccaat ccaagtctga 1680

<210> 111

<211> 559

<212> PRT

<213> 胡椒莓(Drimys lanceolata)_SCH52_13163_6蛋白

<400> 111

Met Asp Val Leu Ile Pro Ser Pro Val Ala Ser Thr Leu Pro Leu Pro

1 5 10 15

Glu Asp Gly Asn Leu Asp Val Val Arg Arg Ser Ala Gly Phe His Pro

20 25 30

Thr Val Trp Gly Asp His Phe Leu Ala Tyr Ser Pro Asp Pro Thr Lys

35 40 45

Ile Asp Ala Trp Thr Lys Arg Val Glu Glu Leu Lys Gln Glu Val Lys

50 55 60

Arg Ile Leu Ser Asn Val Lys Gly Ser Leu Glu Glu Leu Asn Leu Leu

65 70 75 80

Asp Ala Ile Gln His Leu Gly Ile Gly Tyr His Phe Glu Lys Glu Ile

85 90 95

Asp Asp Ala Leu Gln Leu Ile Phe Asp Ser His Ile Asp Ala Phe Pro

100 105 110

Thr Asp Asp Leu Tyr Val Ala Ala Leu Arg Phe Ser Leu Leu Arg Arg

115 120 125

Gln Gly His Cys Val Ser Ser Asp Val Phe Lys Lys Phe Lys Asp Glu

130 135 140

Gln Gly Asn Phe Lys Ala Glu Leu Ser Thr Asp Ala Lys Gly Leu Leu

145 150 155 160

Ser Leu Tyr Asp Ala Ala Tyr Leu Ser Val Arg Gly Glu Asp Ile Leu

165 170 175

Asp Glu Ala Ile Pro Phe Thr Arg Glu His Leu Arg Thr Cys Ile Ser

180 185 190

His Val Asp Ser His Leu Ala Ala Lys Ile Glu His Ser Leu Glu Leu

195 200 205

Pro Leu His His Arg Ile Pro Arg Leu Glu Asn Arg His Tyr Ile Ser

210 215 220

Val Tyr Glu Gly Glu Lys Glu Arg Asn Glu Val Val Leu Glu Leu Ala

225 230 235 240

Lys Leu Asp Phe Asn Leu Ile Gln Ile Leu His Gln Arg Glu Leu Arg

245 250 255

Asp Ile Thr Thr Trp Trp Asn Glu Ile Asp Leu Ala Ala Lys Leu Pro

260 265 270

Phe Ile Arg Asp Arg Leu Val Glu Cys Tyr Tyr Trp Ile Met Gly Val

275 280 285

Tyr Phe Glu Pro Ile Phe Ser Arg Ala Arg Val Phe Ser Thr Lys Met

290 295 300

Thr Ile Leu Val Ser Val Val Asp Asp Ile Tyr Asp Val Tyr Ala Thr

305 310 315 320

Glu Asp Glu Leu Gln Leu Phe Thr Asp Ala Ile Tyr Arg Trp Asp Ala

325 330 335

Glu Asp Ile Glu Gln Leu Pro Gln Tyr Leu Lys Asp Ser Phe Leu Val

340 345 350

Leu Tyr Asn Thr Val Lys Asp Leu Glu Glu Glu Leu Lys Pro Glu Gly

355 360 365

Asn Ser Tyr Arg Gly Asp Tyr Val Lys Asp Ala Met Lys Val Leu Ala

370 375 380

Arg Asp Tyr Phe Val Glu His Lys Trp Tyr Asn Arg Lys Ile Val Pro

385 390 395 400

Ser Val Glu Asp Tyr Leu Arg Ile Ser Cys Ile Ser Val Ala Val His

405 410 415

Met Ala Thr Val His Cys Cys Ala Gly Met Asp Glu Ile Ala Thr Lys

420 425 430

Glu Ala Phe Glu Trp Leu Lys Thr Glu Pro Lys Leu Val Ile Asp Ala

435 440 445

Ser Leu Ile Gly Arg Leu Leu Asp Asp Met Gln Ser Thr Ser Phe Glu

450 455 460

Gln Gln Arg Gly His Val Ser Ser Ala Val Gln Cys Tyr Met Ile Gln

465 470 475 480

Tyr Gly Val Ser His Glu Glu Ala Cys Glu Lys Leu Thr Glu Met Ala

485 490 495

Ala Ile Ala Trp Lys Asp Val Asn Gln Ala Cys Leu Arg Pro Thr Val

500 505 510

Phe Pro Met Pro Ile Leu Leu Pro Ser Ile Asn Leu Ala Arg Val Ala

515 520 525

Glu Val Ile Tyr Leu Arg Gly Asp Gly Tyr Thr His Ala Gly Gly Glu

530 535 540

Thr Lys Lys His Ile Thr Ala Met Leu Val Glu Pro Ile Gln Val

545 550 555

<210> 112

<211> 1680

<212> DNA

<213> 人工序列_SCH51-13163-6的密码子优化的DNA序列

<220>

<223> SCH51-13163-6的密码子优化的DNA序列

<400> 112

atggatgttc tgattccgag tccggttgca agcaccctgc cgctgccgga agatggtaat 60

ctggatgttg ttcgtcgtag cgcaggtttt catccgaccg tttggggtga tcattttctg 120

gcatatagtc cggatccgac caaaattgat gcatggacca aacgtgttga ggaactgaaa 180

caagaagtga aacgtattct gagcaatgtg aaaggtagcc tggaagaact gaatctgctg 240

gatgcaattc agcatctggg tattggttat cacttcgaga aagaaattga tgatgcactg 300

cagctgatct ttgatagcca tattgatgcc tttccgaccg atgatctgta tgttgcagca 360

ctgcgtttta gcctgctgcg tcgtcagggt cattgtgtta gcagtgatgt tttcaaaaaa 420

ttcaaagacg agcagggcaa ctttaaagca gaactgagca ccgatgcaaa aggtctgctg 480

agcctgtatg atgccgcata tctgagcgtt cgtggtgaag atattctgga tgaagcaatt 540

ccgtttaccc gtgaacatct gcgtacctgt attagccatg tggatagcca tctggcagca 600

aaaattgaac atagtctgga actgcctctg catcatcgta ttccgcgtct ggaaaatcgt 660

cactatatta gcgtttatga aggcgaaaaa gaacgcaatg aagttgtgct ggaactggca 720

aaactggatt ttaacctgat tcagattctg catcagcgtg aactgcgtga tattaccacc 780

tggtggaatg aaattgacct ggcagccaaa ctgccgttta ttcgtgatcg tctggttgaa 840

tgctattatt ggattatggg cgtgtatttt gaaccgattt ttagccgtgc acgtgtgttt 900

agcaccaaaa tgaccattct ggttagcgtg gtggatgata tctatgatgt ttatgcaacc 960

gaagatgagc tgcaactgtt taccgatgcc atttatcgtt gggatgcaga agatattgaa 1020

cagctgcctc agtatctgaa agatagcttt ctggttctgt acaacaccgt gaaagatctg 1080

gaagaagaac tgaaaccgga aggtaatagc tatcgtggtg attatgttaa agacgccatg 1140

aaagttctgg cacgcgatta ttttgttgag cacaaatggt ataaccgcaa aattgttccg 1200

agcgtggaag attatctgcg tattagctgc attagcgttg cagttcacat ggcaaccgtt 1260

cattgttgtg caggtatgga tgaaattgca accaaagaag catttgagtg gctgaaaacc 1320

gaaccgaaac tggttattga tgcaagcctg attggtcgtc tgctggacga tatgcagtca 1380

accagctttg aacagcagcg tggtcatgtt agcagcgcag ttcagtgtta tatgattcag 1440

tatggtgtta gccatgaaga agcatgcgaa aaactgaccg aaatggcagc aattgcatgg 1500

aaagatgtta atcaggcatg tctgcgtccg accgtgtttc ctatgccgat tctgctgccg 1560

agcattaatc tggcacgtgt tgccgaagtt atctatctgc gtggtgatgg ttatacccat 1620

gccggtggtg aaaccaaaaa acatattacc gcaatgctgg tagaaccgat tcaggtttaa 1680

<210> 113

<211> 1671

<212> DNA

<213> 黑水缬草(Valeriana amurensis)_VaTPS3

<400> 113

atgtctactg cattaaacag tgagcatgaa actgttcgtc cattagcaag ttttaaaccg 60

agtacatggg gcgatctttt catctcttat tctgaagata gccagcttaa ggaagtatat 120

ggtaaagagc acgaatgtct gaaacaacaa gtgaaaacaa tgttgttgga tctgacaaat 180

tatagaattt cggagaaaat cgctttcata aatacgttgg agagattagg ggtatctcat 240

gagtttgaga atgagattga agggctgctt catcaaatgt ttgatgctca ttctaaattc 300

caagatggca ttcaacactt tgatttgttc acattgggga tttactttag gattctcagg 360

caacatggct atagaatctc ttgtgatgtt ttcaacaagt tgaaagatag caacaatgaa 420

ttcaagaagg aacttaaaga ggacgctatt ggtttgctaa gtttgtacga agcgacacaa 480

gtaagagcac acgctgaaga aattttagac gaagccctca ttttcacaaa ggctcaactt 540

gaatccatag ccgcaacctc gagcttaagc ccatttgtcg agaagcaaat tactcatgct 600

ttggtccaag ctctccacaa aggaatccca agagtcgaat cgcgccattt catctctgtt 660

tatgaagaag atcctgacaa aaatgatttg ttgttgaggt tctcaaagat tgattacaat 720

cttgtacaaa tgcttcacaa gcaagaattg tgccatatct caaagtggtg gagagattcg 780

gagctcgaaa caaaactaac ttatgtgagg aatagagtgg cggaatgctt tttatggact 840

ctttgtgtgt accacgaacc aaagtactct ccggctcggc ttctgttagg caaactcata 900

aatatcatat cttgcactga tgacacatat gatgcgtatg gtacattaga ggaagttcag 960

atctttacag atgtcataca aaggttggat aggagttcta tggagcagct gccggattac 1020

atgaaaatcc tctacaaagc tgtccttgat ctttttgacg aagtagaagt tcagctatcg 1080

aaccatgaaa ctaataatac ttatcgtatg gcttatgcga aggaagagtt aaaagctatc 1140

gccaagtgct acgaaaagga gcacatatgg ttcagaaaat gtcacgtgcc cccattcgaa 1200

gaatatctag agaatgcggt agtgtcaatc ggtaatcgtt tggccgtacc tttttctttt 1260

ctgggaatgg atcaagtagc aggtgttgaa gcgttcgagt gggccaaaac tgatcccaaa 1320

atggtaaaat cgtgcggtaa agtcttacga cttgttgacg atgtaatgag ccacgaggag 1380

gaagatgtaa gaggacacgt ggcaacggga gtcgaatgct acatgaaaga acacggagtg 1440

agtagggaag aggccatcgt ggagttctac aagagggtcg agtacgcgtg gaaggatgtg 1500

aacgaggaat ttataacgcc gaaccatctg catatcgacc tcctcaaccg cgttcttaac 1560

cttacaagaa ttgcagacgt tgtttacaag tttgaagacg gctacacgca tcccgagaag 1620

actctgaaac atcatatcat ggcgttgttc gtcgaccccg tccccatata g 1671

<210> 114

<211> 556

<212> PRT

<213> 黑水缬草(Valeriana amurensis)_VaTPS3蛋白

<400> 114

Met Ser Thr Ala Leu Asn Ser Glu His Glu Thr Val Arg Pro Leu Ala

1 5 10 15

Ser Phe Lys Pro Ser Thr Trp Gly Asp Leu Phe Ile Ser Tyr Ser Glu

20 25 30

Asp Ser Gln Leu Lys Glu Val Tyr Gly Lys Glu His Glu Cys Leu Lys

35 40 45

Gln Gln Val Lys Thr Met Leu Leu Asp Leu Thr Asn Tyr Arg Ile Ser

50 55 60

Glu Lys Ile Ala Phe Ile Asn Thr Leu Glu Arg Leu Gly Val Ser His

65 70 75 80

Glu Phe Glu Asn Glu Ile Glu Gly Leu Leu His Gln Met Phe Asp Ala

85 90 95

His Ser Lys Phe Gln Asp Gly Ile Gln His Phe Asp Leu Phe Thr Leu

100 105 110

Gly Ile Tyr Phe Arg Ile Leu Arg Gln His Gly Tyr Arg Ile Ser Cys

115 120 125

Asp Val Phe Asn Lys Leu Lys Asp Ser Asn Asn Glu Phe Lys Lys Glu

130 135 140

Leu Lys Glu Asp Ala Ile Gly Leu Leu Ser Leu Tyr Glu Ala Thr Gln

145 150 155 160

Val Arg Ala His Ala Glu Glu Ile Leu Asp Glu Ala Leu Ile Phe Thr

165 170 175

Lys Ala Gln Leu Glu Ser Ile Ala Ala Thr Ser Ser Leu Ser Pro Phe

180 185 190

Val Glu Lys Gln Ile Thr His Ala Leu Val Gln Ala Leu His Lys Gly

195 200 205

Ile Pro Arg Val Glu Ser Arg His Phe Ile Ser Val Tyr Glu Glu Asp

210 215 220

Pro Asp Lys Asn Asp Leu Leu Leu Arg Phe Ser Lys Ile Asp Tyr Asn

225 230 235 240

Leu Val Gln Met Leu His Lys Gln Glu Leu Cys His Ile Ser Lys Trp

245 250 255

Trp Arg Asp Ser Glu Leu Glu Thr Lys Leu Thr Tyr Val Arg Asn Arg

260 265 270

Val Ala Glu Cys Phe Leu Trp Thr Leu Cys Val Tyr His Glu Pro Lys

275 280 285

Tyr Ser Pro Ala Arg Leu Leu Leu Gly Lys Leu Ile Asn Ile Ile Ser

290 295 300

Cys Thr Asp Asp Thr Tyr Asp Ala Tyr Gly Thr Leu Glu Glu Val Gln

305 310 315 320

Ile Phe Thr Asp Val Ile Gln Arg Leu Asp Arg Ser Ser Met Glu Gln

325 330 335

Leu Pro Asp Tyr Met Lys Ile Leu Tyr Lys Ala Val Leu Asp Leu Phe

340 345 350

Asp Glu Val Glu Val Gln Leu Ser Asn His Glu Thr Asn Asn Thr Tyr

355 360 365

Arg Met Ala Tyr Ala Lys Glu Glu Leu Lys Ala Ile Ala Lys Cys Tyr

370 375 380

Glu Lys Glu His Ile Trp Phe Arg Lys Cys His Val Pro Pro Phe Glu

385 390 395 400

Glu Tyr Leu Glu Asn Ala Val Val Ser Ile Gly Asn Arg Leu Ala Val

405 410 415

Pro Phe Ser Phe Leu Gly Met Asp Gln Val Ala Gly Val Glu Ala Phe

420 425 430

Glu Trp Ala Lys Thr Asp Pro Lys Met Val Lys Ser Cys Gly Lys Val

435 440 445

Leu Arg Leu Val Asp Asp Val Met Ser His Glu Glu Glu Asp Val Arg

450 455 460

Gly His Val Ala Thr Gly Val Glu Cys Tyr Met Lys Glu His Gly Val

465 470 475 480

Ser Arg Glu Glu Ala Ile Val Glu Phe Tyr Lys Arg Val Glu Tyr Ala

485 490 495

Trp Lys Asp Val Asn Glu Glu Phe Ile Thr Pro Asn His Leu His Ile

500 505 510

Asp Leu Leu Asn Arg Val Leu Asn Leu Thr Arg Ile Ala Asp Val Val

515 520 525

Tyr Lys Phe Glu Asp Gly Tyr Thr His Pro Glu Lys Thr Leu Lys His

530 535 540

His Ile Met Ala Leu Phe Val Asp Pro Val Pro Ile

545 550 555

<210> 115

<211> 1671

<212> DNA

<213> 人工序列_VaTPS3 nt序列，经密码子优化

<220>

<223> 密码子优化的序列

<400> 115

atgagcaccg cgttgaactc cgagcatgaa accgtccgtc cgctggctag ctttaaaccg 60

agcacgtggg gtgacctgtt catcagctac agcgaggaca gccagctgaa agaagtgtat 120

ggtaaagagc atgaatgtct taagcaacaa gttaagacca tgctgctgga cctgacgaat 180

taccgtatca gcgagaagat tgccttcatc aatacgctgg agcgcctggg tgtttctcac 240

gagttcgaga atgaaatcga aggcctcctg catcagatgt tcgacgcgca ctccaagttt 300

caagatggca ttcagcactt tgacctgttt accctgggca tttacttccg tattttgcgc 360

cagcacggtt atcgtatctc gtgcgatgtg tttaacaagc tgaaggactc taataacgaa 420

ttcaagaaag aactgaaaga agatgcaatt ggtctgctgt ctctgtatga agcgacccaa 480

gtgcgtgccc atgcagaaga gattttggac gaagcgctga tcttcaccaa ggctcagctg 540

gagagcatcg cggcgacgag cagcctgagc ccgtttgtcg agaaacagat tacccacgcc 600

ttggtgcaag cgttgcataa aggcatccca cgcgtggaga gccgccactt cattagcgtg 660

tacgaagagg acccggacaa gaacgatttg ctgctgcgtt tttccaagat tgactacaat 720

ttagttcaaa tgctgcacaa acaagagttg tgtcatatta gcaaatggtg gcgtgactcc 780

gagctggaga ctaaactgac ctacgtccgt aatcgcgtgg cagagtgttt tctgtggacc 840

ctgtgtgttt accacgagcc gaagtatagc ccggcacgtc tgctgctggg taaactgatc 900

aacatcattt cttgcacgga cgacacctat gatgcatacg gtacgctgga agaagtccaa 960

atctttaccg acgtgatcca gcgtttggac cgtagctcga tggagcagct gccggattac 1020

atgaagattc tgtataaagc tgttctggat ctgttcgatg aagttgaggt tcagctgagc 1080

aaccatgaga ctaacaatac ctaccgcatg gcgtacgcaa aagaagaact gaaggctatt 1140

gcgaaatgct acgagaaaga gcacatctgg tttcgcaagt gtcatgttcc accgttcgaa 1200

gagtatctgg agaacgccgt ggtgagcatc ggtaatcgtc tggcggtccc gttcagcttc 1260

ttgggtatgg accaggttgc gggcgtcgag gcctttgagt gggcaaagac cgatcctaaa 1320

atggttaaaa gctgcggtaa ggttctgcgc ctggtcgatg atgtcatgag ccatgaagaa 1380

gaagatgtgc gtggtcacgt ggcgacgggc gttgagtgct acatgaaaga gcacggtgtc 1440

agccgtgaag aggcgatcgt tgaattctat aagcgtgtcg agtatgcatg gaaagacgtc 1500

aacgaagagt tcattactcc gaatcacttg cacattgatc tgctgaaccg tgttctgaac 1560

ttaacccgca ttgccgatgt cgtatacaag tttgaagatg gctataccca cccggaaaag 1620

acgctgaaac accatatcat ggcgctgttc gtggacccgg tgccgatcta a 1671

<210> 116

<211> 1332

<212> DNA

<213> 香鳞毛蕨(Dryopteris fragrans)_DfACT13天然核苷酸序列

<400> 116

atggcctgtg gaggcggcgt gaggaatatt gaggtgaagg cgcaggagcc cgttttggtg 60

cagcctttat ctacagcaca aagctctgcc tataatcttc ttacaaaact ggaccagacg 120

ctcgcacaat tggtggtgca gattgtcttt gtcttcgatg tgaaaaaccc cgtaacccgc 180

cgaccccacg atggcgccga tcctgccaag cttctgaagg aggctctgag gaaggtcctt 240

gtgcccttct acccgctcgc tgggcgtctt tgcctctcgc ccgatgacgg cagtctgttc 300

attgactgca atgctcaggg ggtttccttt gttgaggcca atgccgacgc ggatatctcc 360

gaactcggcg acttctcgca gcccgatttc gcgactctgg gctcccttgt cttcccctta 420

cctcctatag cttcggatga tggtcctctt ctatctgcgc aggtgaccag attcaagtgt 480

ggaggatttg tgctgggctt cgtattccat cattgtttat ttgatggatt cgcactttcg 540

gaattcctaa atgcgtgggc ggagactgca tgcggcgtgc ctctttctac acctcctgtc 600

ctcgacagaa cttttccgag ggcgcgttct cccttgcaaa tcaagtatcc ccacaccgag 660

ttcctggaag tcgaagatgt ctctttgact caaaatatct ctaacgatgc catcaaccgg 720

tctttctgct ttacttcggc aagtctagag atactcaaga agaaagcctt ggaggatggg 780

gtgctatcaa aatgcactac ttttgaagct ttatctgggc tgatatggag ggcccgaact 840

agagccctgt ggagcgatta cccggagcac aaactaaagg tgctcattgt cgtcgacccg 900

agagcacgtt ttgagcctcg tgtggtgcca aaagggtatg tgggcaatgc ggtgcttttt 960

acatgtgctt tcgcaagcgc aagggagctg gaagaaaatc ccttgtcaca tgcagtgaag 1020

catgtgcaac atgccatcgg gcgtatgacg gaggagtaca tgttgtcgca aatcgactac 1080

atggagcatc agaaggtatg gtgcccaccg ctaggagcta gtacatcttt catgaccaaa 1140

tggtctaggt tggccttcaa tattctagac tttgggtggg gcaggccgaa gtatgtgggg 1200

ccggccacgt cgctgtcgat ggagacgact acttttgtat cttatggaaa gggcatgagt 1260

gtggtgttgg ctcttcctcc agaagcaatg cgcaaatttg aaaaaatcgt acatccctac 1320

ctcaatccat ga 1332

<210> 117

<211> 1332

<212> DNA

<213> 人工_DfACT13_针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1332)

<400> 117

atg gca tgt ggc ggt ggg gtc agg aat atc gaa gtg aag gct caa gag 48

Met Ala Cys Gly Gly Gly Val Arg Asn Ile Glu Val Lys Ala Gln Glu

1 5 10 15

cca gtc ttg gtg caa ccg tta agc act gct caa tcg tca gcc tac aat 96

Pro Val Leu Val Gln Pro Leu Ser Thr Ala Gln Ser Ser Ala Tyr Asn

20 25 30

ttg tta aca aag tta gac cag act ttg gcg caa ttg gtt gtc caa ata 144

Leu Leu Thr Lys Leu Asp Gln Thr Leu Ala Gln Leu Val Val Gln Ile

35 40 45

gtg ttt gtt ttc gat gtg aag aac cct gtg aca aga agg cct cat gat 192

Val Phe Val Phe Asp Val Lys Asn Pro Val Thr Arg Arg Pro His Asp

50 55 60

gga gcc gat cca gct aag tta ttg aaa gag gcc tta agg aaa gta cta 240

Gly Ala Asp Pro Ala Lys Leu Leu Lys Glu Ala Leu Arg Lys Val Leu

65 70 75 80

gta cca ttc tat ccc ctt gcc gga aga cta tgt ctt tcc cca gac gat 288

Val Pro Phe Tyr Pro Leu Ala Gly Arg Leu Cys Leu Ser Pro Asp Asp

85 90 95

ggt agt cta ttc ata gac tgc aac gct caa ggt gtt tca ttt gtc gaa 336

Gly Ser Leu Phe Ile Asp Cys Asn Ala Gln Gly Val Ser Phe Val Glu

100 105 110

gca aac gct gat gcc gac atc agt gag tta ggt gat ttc tcc caa ccg 384

Ala Asn Ala Asp Ala Asp Ile Ser Glu Leu Gly Asp Phe Ser Gln Pro

115 120 125

gat ttt gcg acc tta ggt tct tta gtc ttc cct ctt cca cca att gcg 432

Asp Phe Ala Thr Leu Gly Ser Leu Val Phe Pro Leu Pro Pro Ile Ala

130 135 140

tct gac gat gga cca ttg tta agt gct caa gtc aca aga ttc aaa tgt 480

Ser Asp Asp Gly Pro Leu Leu Ser Ala Gln Val Thr Arg Phe Lys Cys

145 150 155 160

ggt ggc ttc gtt cta ggt ttc gtc ttc cat cac tgt cta ttt gat ggt 528

Gly Gly Phe Val Leu Gly Phe Val Phe His His Cys Leu Phe Asp Gly

165 170 175

ttt gcc tta tct gaa ttt ctt aat gca tgg gct gag aca gcg tgt gga 576

Phe Ala Leu Ser Glu Phe Leu Asn Ala Trp Ala Glu Thr Ala Cys Gly

180 185 190

gtc ccg tta tca acc cca cct gtt tta gat aga aca ttt cct cgt gct 624

Val Pro Leu Ser Thr Pro Pro Val Leu Asp Arg Thr Phe Pro Arg Ala

195 200 205

aga tct ccc ctg caa ata aag tac cca cat aca gag ttc cta gag gta 672

Arg Ser Pro Leu Gln Ile Lys Tyr Pro His Thr Glu Phe Leu Glu Val

210 215 220

gaa gat gtt tca tta act cag aac atc tct aac gat gct atc aac aga 720

Glu Asp Val Ser Leu Thr Gln Asn Ile Ser Asn Asp Ala Ile Asn Arg

225 230 235 240

tcc ttc tgt ttt aca tca gcc tct ttg gaa att ttg aag aag aag gcg 768

Ser Phe Cys Phe Thr Ser Ala Ser Leu Glu Ile Leu Lys Lys Lys Ala

245 250 255

cta gaa gac ggg gtg tta agc aaa tgt acg acc ttc gaa gct cta tct 816

Leu Glu Asp Gly Val Leu Ser Lys Cys Thr Thr Phe Glu Ala Leu Ser

260 265 270

ggc tta ata tgg aga gca cgt acc aga gcc tta tgg agc gac tac ccc 864

Gly Leu Ile Trp Arg Ala Arg Thr Arg Ala Leu Trp Ser Asp Tyr Pro

275 280 285

gag cac aag tta aag gtc ctg att gtc gtg gac cct cgt gcc aga ttt 912

Glu His Lys Leu Lys Val Leu Ile Val Val Asp Pro Arg Ala Arg Phe

290 295 300

gaa ccg aga gtg gtg cca aaa ggc tac gta ggg aat gca gtc ttg ttt 960

Glu Pro Arg Val Val Pro Lys Gly Tyr Val Gly Asn Ala Val Leu Phe

305 310 315 320

act tgt gca ttt gct tca gcc aga gaa cta gaa gaa aat cca tta tct 1008

Thr Cys Ala Phe Ala Ser Ala Arg Glu Leu Glu Glu Asn Pro Leu Ser

325 330 335

cat gct gtt aaa cac gta cag cac gcg atc ggc cgt atg act gag gaa 1056

His Ala Val Lys His Val Gln His Ala Ile Gly Arg Met Thr Glu Glu

340 345 350

tat atg cta tca caa att gat tac atg gag cac cag aag gtt tgg tgt 1104

Tyr Met Leu Ser Gln Ile Asp Tyr Met Glu His Gln Lys Val Trp Cys

355 360 365

ccc cca cta ggt gcg tcg act tct ttt atg act aag tgg tca agg ctt 1152

Pro Pro Leu Gly Ala Ser Thr Ser Phe Met Thr Lys Trp Ser Arg Leu

370 375 380

gcc ttt aac atc tta gat ttt ggt tgg ggt aga cct aag tat gtt ggt 1200

Ala Phe Asn Ile Leu Asp Phe Gly Trp Gly Arg Pro Lys Tyr Val Gly

385 390 395 400

ccg gct act tca ttg tct atg gaa aca act aca ttc gtc tcc tat gga 1248

Pro Ala Thr Ser Leu Ser Met Glu Thr Thr Thr Phe Val Ser Tyr Gly

405 410 415

aag ggt atg agc gtg gtg tta gcc cta ccc cca gag gca atg aga aag 1296

Lys Gly Met Ser Val Val Leu Ala Leu Pro Pro Glu Ala Met Arg Lys

420 425 430

ttc gaa aag att gta cac cct tat ttg aac cct taa 1332

Phe Glu Lys Ile Val His Pro Tyr Leu Asn Pro

435 440

<210> 118

<211> 443

<212> PRT

<213> 人工_DfACT13_针对其在酿酒酵母中的表达经密码子优化

<400> 118

Met Ala Cys Gly Gly Gly Val Arg Asn Ile Glu Val Lys Ala Gln Glu

1 5 10 15

Pro Val Leu Val Gln Pro Leu Ser Thr Ala Gln Ser Ser Ala Tyr Asn

20 25 30

Leu Leu Thr Lys Leu Asp Gln Thr Leu Ala Gln Leu Val Val Gln Ile

35 40 45

Val Phe Val Phe Asp Val Lys Asn Pro Val Thr Arg Arg Pro His Asp

50 55 60

Gly Ala Asp Pro Ala Lys Leu Leu Lys Glu Ala Leu Arg Lys Val Leu

65 70 75 80

Val Pro Phe Tyr Pro Leu Ala Gly Arg Leu Cys Leu Ser Pro Asp Asp

85 90 95

Gly Ser Leu Phe Ile Asp Cys Asn Ala Gln Gly Val Ser Phe Val Glu

100 105 110

Ala Asn Ala Asp Ala Asp Ile Ser Glu Leu Gly Asp Phe Ser Gln Pro

115 120 125

Asp Phe Ala Thr Leu Gly Ser Leu Val Phe Pro Leu Pro Pro Ile Ala

130 135 140

Ser Asp Asp Gly Pro Leu Leu Ser Ala Gln Val Thr Arg Phe Lys Cys

145 150 155 160

Gly Gly Phe Val Leu Gly Phe Val Phe His His Cys Leu Phe Asp Gly

165 170 175

Phe Ala Leu Ser Glu Phe Leu Asn Ala Trp Ala Glu Thr Ala Cys Gly

180 185 190

Val Pro Leu Ser Thr Pro Pro Val Leu Asp Arg Thr Phe Pro Arg Ala

195 200 205

Arg Ser Pro Leu Gln Ile Lys Tyr Pro His Thr Glu Phe Leu Glu Val

210 215 220

Glu Asp Val Ser Leu Thr Gln Asn Ile Ser Asn Asp Ala Ile Asn Arg

225 230 235 240

Ser Phe Cys Phe Thr Ser Ala Ser Leu Glu Ile Leu Lys Lys Lys Ala

245 250 255

Leu Glu Asp Gly Val Leu Ser Lys Cys Thr Thr Phe Glu Ala Leu Ser

260 265 270

Gly Leu Ile Trp Arg Ala Arg Thr Arg Ala Leu Trp Ser Asp Tyr Pro

275 280 285

Glu His Lys Leu Lys Val Leu Ile Val Val Asp Pro Arg Ala Arg Phe

290 295 300

Glu Pro Arg Val Val Pro Lys Gly Tyr Val Gly Asn Ala Val Leu Phe

305 310 315 320

Thr Cys Ala Phe Ala Ser Ala Arg Glu Leu Glu Glu Asn Pro Leu Ser

325 330 335

His Ala Val Lys His Val Gln His Ala Ile Gly Arg Met Thr Glu Glu

340 345 350

Tyr Met Leu Ser Gln Ile Asp Tyr Met Glu His Gln Lys Val Trp Cys

355 360 365

Pro Pro Leu Gly Ala Ser Thr Ser Phe Met Thr Lys Trp Ser Arg Leu

370 375 380

Ala Phe Asn Ile Leu Asp Phe Gly Trp Gly Arg Pro Lys Tyr Val Gly

385 390 395 400

Pro Ala Thr Ser Leu Ser Met Glu Thr Thr Thr Phe Val Ser Tyr Gly

405 410 415

Lys Gly Met Ser Val Val Leu Ala Leu Pro Pro Glu Ala Met Arg Lys

420 425 430

Phe Glu Lys Ile Val His Pro Tyr Leu Asn Pro

435 440

<210> 119

<211> 1440

<212> DNA

<213> 碳核曲霉(Aspergillus sclerotiicarbonarius)_PYI04555.1天然核苷酸序列

<400> 119

atgggtgcca gcgtctcttt ccagcccttt gtgcccactc ccctggacca tgctatgccc 60

ccaatttacg tgtctcaatt tctatgcttt cctaccacaa ctccgcaatc cgctattcaa 120

agtctccaag tgggaatcga gagattattc gaacgcctgc cattcctggc gggagagatt 180

ctcatcaatg aacacaccgg agccatcaaa gtccaggctc ccagtgcttt gatccgggaa 240

attccctaca tggccctccg agcccatcct gatctttacc ttccagctaa gcaatgtgca 300

accacaccaa tcgagagaca gttgaagacc aacagccttg atgaatccta ccatccactc 360

ccggcggcac ttccactctc ccaaccccag cccgtcatcc gattccaagc aaacaccctc 420

gcagacggca ttctctttgc agtcagctat catcattgca tattcgatgg cactggatgc 480

ggacagattc tggaaatgtt ggctcagtgc tgttcggcct ccgacgataa gatctccctg 540

ccgactgatt gccacaccga tgtgctcctc cgcgaataca tctccaatct aagccctact 600

accaacatcc cccacgatta cacgcaagcg tatagcacta cggtgcaacc ggaccccgat 660

gcctcagacc cagacacgtc ccccgccata ccctcctcac tctacacaga agcattcacc 720

tttccctccc aacaaatcac cactctccgt gatgcatgca accacctctt gcccaaatta 780

cccagcacca gcaacgcaca tccccacaaa ccaacaccga atcccctatc atcaaatgac 840

gtcctcaccg cactaatagc cctatgcatc acacgcgcca ccaacaccac caccccaccc 900

ctccaaccca acaatcacag tctctcaatg gccgtcaacc tccgaacccg catccagccc 960

caagtaccag atcactttct gggaaacttc gccacgctac tcccaataca ctttaccagt 1020

ccagtccaca cccaacagtc tgatctcctc ctcaccacag aaccccccga ccccgccctc 1080

atccacctaa ccaccctcgc ctcccaaatc cggtccagcc tatccacagt caacaccgat 1140

tacatctgcg gtctcatgac ggatctccga acccggcgca atgcaggaga aaacagcagt 1200

cttctaattg aaggcattaa gatttccagt tggagacatc tatccgtcta caagccggac 1260

tttggccctg ggttggggaa aatagcgggg tttgagttcc aggcggggct tatggataat 1320

ttggtggtga ttttgccttg gagaaatggg gattgggatg tgcgtgttac gttgttggag 1380

agggatatgc gcgggtttag ggaggatcgg ttggttaggt gggcgttggg gtctgggtag 1440

<210> 120

<211> 1470

<212> DNA

<213> 人工_PYI04555.1_针对其在酿酒酵母中的表达经密码子优化_C-末端扩展

<220>

<221> CDS

<222> (1)..(1470)

<400> 120

atg ggt gcg tca gtt agt ttt caa cct ttc gtt cct acc ccc ttg gat 48

Met Gly Ala Ser Val Ser Phe Gln Pro Phe Val Pro Thr Pro Leu Asp

1 5 10 15

cac gcc atg cca ccg ata tac gtg tca cag ttc ttg tgt ttc cct act 96

His Ala Met Pro Pro Ile Tyr Val Ser Gln Phe Leu Cys Phe Pro Thr

20 25 30

acg acg ccg cag tca gca ata caa tcc cta cag gtc ggt att gag cgt 144

Thr Thr Pro Gln Ser Ala Ile Gln Ser Leu Gln Val Gly Ile Glu Arg

35 40 45

ttg ttt gaa aga cta cct ttt cta gcc ggg gaa att ctg ata aat gag 192

Leu Phe Glu Arg Leu Pro Phe Leu Ala Gly Glu Ile Leu Ile Asn Glu

50 55 60

cac aca ggt gca ata aaa gtt caa gct ccc tct gct ctg att aga gaa 240

His Thr Gly Ala Ile Lys Val Gln Ala Pro Ser Ala Leu Ile Arg Glu

65 70 75 80

att ccg tac atg gcc tta agg gct cat ccg gat ttg tat ctg cct gct 288

Ile Pro Tyr Met Ala Leu Arg Ala His Pro Asp Leu Tyr Leu Pro Ala

85 90 95

aag caa tgt gca act act cca ata gag agg cag ttg aaa act aac agc 336

Lys Gln Cys Ala Thr Thr Pro Ile Glu Arg Gln Leu Lys Thr Asn Ser

100 105 110

ctt gat gag tct tat cat cca tta cct gcg gca cta cca ttg tct caa 384

Leu Asp Glu Ser Tyr His Pro Leu Pro Ala Ala Leu Pro Leu Ser Gln

115 120 125

cca caa cca gtc atc aga ttc caa gct aat aca ctt gct gac gga ata 432

Pro Gln Pro Val Ile Arg Phe Gln Ala Asn Thr Leu Ala Asp Gly Ile

130 135 140

ctt ttc gct gtc agt tac cac cat tgt ata ttt gat gga acg ggc tgc 480

Leu Phe Ala Val Ser Tyr His His Cys Ile Phe Asp Gly Thr Gly Cys

145 150 155 160

gga cag atc ctt gaa atg tta gcc cag tgt tgt tcg gcc tca gac gat 528

Gly Gln Ile Leu Glu Met Leu Ala Gln Cys Cys Ser Ala Ser Asp Asp

165 170 175

aag atc agc ttg cct acg gac tgt cat aca gat gta ttg ttg aga gaa 576

Lys Ile Ser Leu Pro Thr Asp Cys His Thr Asp Val Leu Leu Arg Glu

180 185 190

tat att tcg aat cta tct cca acc acg aat atc ccg cat gac tac act 624

Tyr Ile Ser Asn Leu Ser Pro Thr Thr Asn Ile Pro His Asp Tyr Thr

195 200 205

caa gct tat tct act aca gtt caa cca gat cct gac gca tcc gat cct 672

Gln Ala Tyr Ser Thr Thr Val Gln Pro Asp Pro Asp Ala Ser Asp Pro

210 215 220

gat act agc cca gcc att ccg agt tcg ctg tac act gaa gca ttt acg 720

Asp Thr Ser Pro Ala Ile Pro Ser Ser Leu Tyr Thr Glu Ala Phe Thr

225 230 235 240

ttt cct tca caa cag att acc aca cta aga gat gcg tgc aac cat tta 768

Phe Pro Ser Gln Gln Ile Thr Thr Leu Arg Asp Ala Cys Asn His Leu

245 250 255

ttg cct aaa tta cca tct act tca aac gca cat ccc cac aaa cca aca 816

Leu Pro Lys Leu Pro Ser Thr Ser Asn Ala His Pro His Lys Pro Thr

260 265 270

cca aac ccg tta tcc tca aat gac gtt ttg acg gca ctg atc gca ttg 864

Pro Asn Pro Leu Ser Ser Asn Asp Val Leu Thr Ala Leu Ile Ala Leu

275 280 285

tgc atc acc aga gct act aat acg acg aca ccc cca tta caa cca aac 912

Cys Ile Thr Arg Ala Thr Asn Thr Thr Thr Pro Pro Leu Gln Pro Asn

290 295 300

aac cat tct ctt agc atg gcc gtc aat cta agg act cgt att caa ccc 960

Asn His Ser Leu Ser Met Ala Val Asn Leu Arg Thr Arg Ile Gln Pro

305 310 315 320

caa gtc ccg gac cac ttt ctt ggt aat ttt gcc acc ttg cta cct att 1008

Gln Val Pro Asp His Phe Leu Gly Asn Phe Ala Thr Leu Leu Pro Ile

325 330 335

cac ttc aca agt cca gtc cat acg cag caa tca gat tta tta ttg act 1056

His Phe Thr Ser Pro Val His Thr Gln Gln Ser Asp Leu Leu Leu Thr

340 345 350

act gag ccg cca gac cca gca ttg atc cac ctt aca acc ctt gca tcg 1104

Thr Glu Pro Pro Asp Pro Ala Leu Ile His Leu Thr Thr Leu Ala Ser

355 360 365

caa att agg tct agt tta tcg acc gtt aac acc gat tac ata tgt ggc 1152

Gln Ile Arg Ser Ser Leu Ser Thr Val Asn Thr Asp Tyr Ile Cys Gly

370 375 380

ttg atg aca gat ctg agg act agg aga aac gcc ggt gag aat tcc tct 1200

Leu Met Thr Asp Leu Arg Thr Arg Arg Asn Ala Gly Glu Asn Ser Ser

385 390 395 400

ttg tta ata gaa ggt att aag att tct tca tgg aga cat ctt agc gtt 1248

Leu Leu Ile Glu Gly Ile Lys Ile Ser Ser Trp Arg His Leu Ser Val

405 410 415

tac aag cca gat ttc gga cct gga tta ggg aag att gca gga ttt gag 1296

Tyr Lys Pro Asp Phe Gly Pro Gly Leu Gly Lys Ile Ala Gly Phe Glu

420 425 430

ttt caa gct ggt tta atg gac aac ctt gtt gtg ata ttg cct tgg aga 1344

Phe Gln Ala Gly Leu Met Asp Asn Leu Val Val Ile Leu Pro Trp Arg

435 440 445

aac ggt gat tgg gac gtg cgt gta act ctt ctg gag aga gat atg agg 1392

Asn Gly Asp Trp Asp Val Arg Val Thr Leu Leu Glu Arg Asp Met Arg

450 455 460

ggt ttt agg gaa gac aga tta gtc aga tgg gca tta ggt agc gga aca 1440

Gly Phe Arg Glu Asp Arg Leu Val Arg Trp Ala Leu Gly Ser Gly Thr

465 470 475 480

ggc ccc ttt tcc ttt gtc gat atc atg taa 1470

Gly Pro Phe Ser Phe Val Asp Ile Met

485

<210> 121

<211> 489

<212> PRT

<213> 人工_PYI04555.1_针对其在酿酒酵母中的表达经密码子优化_C-末端扩展

<400> 121

Met Gly Ala Ser Val Ser Phe Gln Pro Phe Val Pro Thr Pro Leu Asp

1 5 10 15

His Ala Met Pro Pro Ile Tyr Val Ser Gln Phe Leu Cys Phe Pro Thr

20 25 30

Thr Thr Pro Gln Ser Ala Ile Gln Ser Leu Gln Val Gly Ile Glu Arg

35 40 45

Leu Phe Glu Arg Leu Pro Phe Leu Ala Gly Glu Ile Leu Ile Asn Glu

50 55 60

His Thr Gly Ala Ile Lys Val Gln Ala Pro Ser Ala Leu Ile Arg Glu

65 70 75 80

Ile Pro Tyr Met Ala Leu Arg Ala His Pro Asp Leu Tyr Leu Pro Ala

85 90 95

Lys Gln Cys Ala Thr Thr Pro Ile Glu Arg Gln Leu Lys Thr Asn Ser

100 105 110

Leu Asp Glu Ser Tyr His Pro Leu Pro Ala Ala Leu Pro Leu Ser Gln

115 120 125

Pro Gln Pro Val Ile Arg Phe Gln Ala Asn Thr Leu Ala Asp Gly Ile

130 135 140

Leu Phe Ala Val Ser Tyr His His Cys Ile Phe Asp Gly Thr Gly Cys

145 150 155 160

Gly Gln Ile Leu Glu Met Leu Ala Gln Cys Cys Ser Ala Ser Asp Asp

165 170 175

Lys Ile Ser Leu Pro Thr Asp Cys His Thr Asp Val Leu Leu Arg Glu

180 185 190

Tyr Ile Ser Asn Leu Ser Pro Thr Thr Asn Ile Pro His Asp Tyr Thr

195 200 205

Gln Ala Tyr Ser Thr Thr Val Gln Pro Asp Pro Asp Ala Ser Asp Pro

210 215 220

Asp Thr Ser Pro Ala Ile Pro Ser Ser Leu Tyr Thr Glu Ala Phe Thr

225 230 235 240

Phe Pro Ser Gln Gln Ile Thr Thr Leu Arg Asp Ala Cys Asn His Leu

245 250 255

Leu Pro Lys Leu Pro Ser Thr Ser Asn Ala His Pro His Lys Pro Thr

260 265 270

Pro Asn Pro Leu Ser Ser Asn Asp Val Leu Thr Ala Leu Ile Ala Leu

275 280 285

Cys Ile Thr Arg Ala Thr Asn Thr Thr Thr Pro Pro Leu Gln Pro Asn

290 295 300

Asn His Ser Leu Ser Met Ala Val Asn Leu Arg Thr Arg Ile Gln Pro

305 310 315 320

Gln Val Pro Asp His Phe Leu Gly Asn Phe Ala Thr Leu Leu Pro Ile

325 330 335

His Phe Thr Ser Pro Val His Thr Gln Gln Ser Asp Leu Leu Leu Thr

340 345 350

Thr Glu Pro Pro Asp Pro Ala Leu Ile His Leu Thr Thr Leu Ala Ser

355 360 365

Gln Ile Arg Ser Ser Leu Ser Thr Val Asn Thr Asp Tyr Ile Cys Gly

370 375 380

Leu Met Thr Asp Leu Arg Thr Arg Arg Asn Ala Gly Glu Asn Ser Ser

385 390 395 400

Leu Leu Ile Glu Gly Ile Lys Ile Ser Ser Trp Arg His Leu Ser Val

405 410 415

Tyr Lys Pro Asp Phe Gly Pro Gly Leu Gly Lys Ile Ala Gly Phe Glu

420 425 430

Phe Gln Ala Gly Leu Met Asp Asn Leu Val Val Ile Leu Pro Trp Arg

435 440 445

Asn Gly Asp Trp Asp Val Arg Val Thr Leu Leu Glu Arg Asp Met Arg

450 455 460

Gly Phe Arg Glu Asp Arg Leu Val Arg Trp Ala Leu Gly Ser Gly Thr

465 470 475 480

Gly Pro Phe Ser Phe Val Asp Ile Met

485

<210> 122

<211> 1425

<212> DNA

<213> 鞭苔(Bazzania trilobata)_ERR364415-1_contig_8546天然核苷酸序列

<400> 122

atggcccggg caccaccacc tccgcctcct ggtctcagaa tgagagacac agtgctcagc 60

atcgtgaagc cgatacggaa gacacagcat ttggagacga tcgacgcaac gttcgtcgat 120

ttgatgagaa tggacagctt tatacccgtg attttcgcct acaggcctgc ggacaagtcc 180

gaggccgcgt actcgcgtct cgtgaatcgc atcaaggagt cgctccagaa ggttttggtc 240

ccctttttcg ggttcgcggg ccgctgggtt ccaagcagtg gcgggagcag gcggctcttg 300

tgcaacgatg agggcgttcc cttcattgaa gcgtttgtgg acgaagagtt ggactcggtg 360

gtgaaggctt ccgccgcatt ccagccggtt acggagctga atggcttggg cgtcctcgga 420

atggacatga cttcatacga tcaaaggatg ccaccggaag gtgggcaacc ttgcgtcgtt 480

gctcaagtca cacggttcaa atgtggggga gtggttctgg gggtggcttt caatcacact 540

cacactgacg gccagggatt ctacaccttc atgcgagcat ggtccgactt ctctcgaacc 600

aacggaacgg caatcaaggt ggaccacaac cgggccctgc cagaactggc ttccctctca 660

cagttcttca tcaaacagca cgaccgaata ggaggcaaaa cttctaccga tcgagtcaac 720

gatcattgtt ctaaagttcc ggaacggctg gctttgaaag ctttcgaggt tcgtgcgtct 780

aagatcaaag ccgcaaagct agcagccgaa gatggagggg ttgggtatgt cagcacggta 840

gattgcattg tggctcactt atggaaaact cttgccagat tgccgcccgt cgtgttggat 900

gggagggaga ttacggtctt ctcgcctgtg gaggggagga acagattctt ggacccgcca 960

agacccaata tgtgtggaaa ttgttttgca gcaatggtga cccccaaaat cccaacccag 1020

gagttgctgg agatgcctct cgctgcaatt gcaggcaagc aacgggagaa attatccaca 1080

acccgaaggg aggaatggtt tggacagcaa agctttaggg agctggcctc cgcgatgaac 1140

accagcaaat ctgctctact tattgtgacc tcgtggttca actttcccat gtatgagatc 1200

gactttggag ctggcaaacc attttttgca tccactacga acatgatttc tcctatcaac 1260

ggcgtgtgtt gtggagtcat tgcaccccca actcctggga gctgctcctc cattgccact 1320

ctgtacattt tgtgccttcc cgcggtacta gaggctcttg aaaatgttcc agatttccta 1380

tccttcttcg ttcctcaccc aaatcacaaa gataactcgc aatag 1425

<210> 123

<211> 1455

<212> DNA

<213> 人工_ERR364415-1_contig_8546_针对其在酿酒酵母中的表达经密码子优化_C-末端扩展

<220>

<221> CDS

<222> (1)..(1455)

<400> 123

atg gcc agg gct cca ccc cca cca cca ccg ggc ttg cgt atg aga gat 48

Met Ala Arg Ala Pro Pro Pro Pro Pro Pro Gly Leu Arg Met Arg Asp

1 5 10 15

acg gtc cta tca atc gtc aaa cct ata aga aag act caa cac cta gag 96

Thr Val Leu Ser Ile Val Lys Pro Ile Arg Lys Thr Gln His Leu Glu

20 25 30

aca att gat gca aca ttc gta gat tta atg aga atg gac tca ttt atc 144

Thr Ile Asp Ala Thr Phe Val Asp Leu Met Arg Met Asp Ser Phe Ile

35 40 45

cct gtg atc ttc gct tat aga cct gca gat aag agc gag gct gcg tac 192

Pro Val Ile Phe Ala Tyr Arg Pro Ala Asp Lys Ser Glu Ala Ala Tyr

50 55 60

tct aga tta gtt aat agg ata aaa gaa agc tta cag aaa gta cta gta 240

Ser Arg Leu Val Asn Arg Ile Lys Glu Ser Leu Gln Lys Val Leu Val

65 70 75 80

cca ttc ttt gga ttt gct gga agg tgg gtg cct tct tcc ggt ggc tca 288

Pro Phe Phe Gly Phe Ala Gly Arg Trp Val Pro Ser Ser Gly Gly Ser

85 90 95

agg cgt ctg cta tgc aat gac gaa ggt gtc cct ttt att gag gcg ttt 336

Arg Arg Leu Leu Cys Asn Asp Glu Gly Val Pro Phe Ile Glu Ala Phe

100 105 110

gta gac gaa gaa ctg gac tct gtc gtt aag gct tca gct gcc ttc caa 384

Val Asp Glu Glu Leu Asp Ser Val Val Lys Ala Ser Ala Ala Phe Gln

115 120 125

cct gta act gaa ctt aat ggt cta ggt gtg ttg ggt atg gat atg act 432

Pro Val Thr Glu Leu Asn Gly Leu Gly Val Leu Gly Met Asp Met Thr

130 135 140

agt tat gat caa aga atg ccc cct gaa ggc ggt caa ccg tgc gtc gta 480

Ser Tyr Asp Gln Arg Met Pro Pro Glu Gly Gly Gln Pro Cys Val Val

145 150 155 160

gct cag gta acg aga ttt aaa tgc ggc ggt gtg gta ttg ggt gta gca 528

Ala Gln Val Thr Arg Phe Lys Cys Gly Gly Val Val Leu Gly Val Ala

165 170 175

ttc aac cat acc cat act gac ggg cag ggc ttt tat act ttt atg cgt 576

Phe Asn His Thr His Thr Asp Gly Gln Gly Phe Tyr Thr Phe Met Arg

180 185 190

gca tgg tcg gat ttc agt aga acg aat ggt acc gca att aaa gtc gat 624

Ala Trp Ser Asp Phe Ser Arg Thr Asn Gly Thr Ala Ile Lys Val Asp

195 200 205

cac aac agg gca cta ccc gaa tta gca tct ttg agt cag ttc ttt att 672

His Asn Arg Ala Leu Pro Glu Leu Ala Ser Leu Ser Gln Phe Phe Ile

210 215 220

aag cag cat gat aga att ggt ggg aaa act tcc acc gat aga gtc aat 720

Lys Gln His Asp Arg Ile Gly Gly Lys Thr Ser Thr Asp Arg Val Asn

225 230 235 240

gac cac tgt agc aaa gtc cct gaa aga cta gca cta aag gct ttt gaa 768

Asp His Cys Ser Lys Val Pro Glu Arg Leu Ala Leu Lys Ala Phe Glu

245 250 255

gtt agg gcg tcc aaa atc aag gca gca aaa cta gcc gca gaa gat ggt 816

Val Arg Ala Ser Lys Ile Lys Ala Ala Lys Leu Ala Ala Glu Asp Gly

260 265 270

ggt gta ggc tac gtc tcg acg gtg gat tgt att gtt gct cat cta tgg 864

Gly Val Gly Tyr Val Ser Thr Val Asp Cys Ile Val Ala His Leu Trp

275 280 285

aag aca cta gct cgt ttg cca cca gtt gtg tta gac ggt cgt gag atc 912

Lys Thr Leu Ala Arg Leu Pro Pro Val Val Leu Asp Gly Arg Glu Ile

290 295 300

act gtg ttt agc cca gta gaa ggc aga aat agg ttt ctt gat ccg ccc 960

Thr Val Phe Ser Pro Val Glu Gly Arg Asn Arg Phe Leu Asp Pro Pro

305 310 315 320

cgt ccg aac atg tgt ggt aat tgt ttc gct gca atg gta acc cct aaa 1008

Arg Pro Asn Met Cys Gly Asn Cys Phe Ala Ala Met Val Thr Pro Lys

325 330 335

atc cca aca caa gaa ttg tta gag atg cca tta gcc gcc att gcc ggc 1056

Ile Pro Thr Gln Glu Leu Leu Glu Met Pro Leu Ala Ala Ile Ala Gly

340 345 350

aag cag aga gag aaa cta tct acc acg aga cgt gaa gag tgg ttc gga 1104

Lys Gln Arg Glu Lys Leu Ser Thr Thr Arg Arg Glu Glu Trp Phe Gly

355 360 365

cag caa tca ttc agg gag ttg gct tca gct atg aat act tct aaa tca 1152

Gln Gln Ser Phe Arg Glu Leu Ala Ser Ala Met Asn Thr Ser Lys Ser

370 375 380

gct ttg tta atc gtg aca tct tgg ttt aac ttc ccg atg tat gaa atc 1200

Ala Leu Leu Ile Val Thr Ser Trp Phe Asn Phe Pro Met Tyr Glu Ile

385 390 395 400

gat ttt ggt gcc ggt aag ccg ttc ttc gct agt acg act aat atg att 1248

Asp Phe Gly Ala Gly Lys Pro Phe Phe Ala Ser Thr Thr Asn Met Ile

405 410 415

tct cct ata aat gga gtt tgt tgt ggt gtc ata gcc ccg ccc acc ccc 1296

Ser Pro Ile Asn Gly Val Cys Cys Gly Val Ile Ala Pro Pro Thr Pro

420 425 430

ggt tcc tgt tca tcc ata gcg aca tta tac att tta tgt tta cca gcc 1344

Gly Ser Cys Ser Ser Ile Ala Thr Leu Tyr Ile Leu Cys Leu Pro Ala

435 440 445

gtg tta gaa gct ctt gaa aat gtc cca gat ttc ctt tcg ttc ttc gta 1392

Val Leu Glu Ala Leu Glu Asn Val Pro Asp Phe Leu Ser Phe Phe Val

450 455 460

ccg cat cca aac cat aaa gac aac agc caa aca ggc ccc ttt tcc ttt 1440

Pro His Pro Asn His Lys Asp Asn Ser Gln Thr Gly Pro Phe Ser Phe

465 470 475 480

gtc gat atc atg taa 1455

Val Asp Ile Met

<210> 124

<211> 484

<212> PRT

<213> 人工_ERR364415-1_contig_8546_针对其在酿酒酵母中的表达经密码子优化_C-末端扩展

<400> 124

Met Ala Arg Ala Pro Pro Pro Pro Pro Pro Gly Leu Arg Met Arg Asp

1 5 10 15

Thr Val Leu Ser Ile Val Lys Pro Ile Arg Lys Thr Gln His Leu Glu

20 25 30

Thr Ile Asp Ala Thr Phe Val Asp Leu Met Arg Met Asp Ser Phe Ile

35 40 45

Pro Val Ile Phe Ala Tyr Arg Pro Ala Asp Lys Ser Glu Ala Ala Tyr

50 55 60

Ser Arg Leu Val Asn Arg Ile Lys Glu Ser Leu Gln Lys Val Leu Val

65 70 75 80

Pro Phe Phe Gly Phe Ala Gly Arg Trp Val Pro Ser Ser Gly Gly Ser

85 90 95

Arg Arg Leu Leu Cys Asn Asp Glu Gly Val Pro Phe Ile Glu Ala Phe

100 105 110

Val Asp Glu Glu Leu Asp Ser Val Val Lys Ala Ser Ala Ala Phe Gln

115 120 125

Pro Val Thr Glu Leu Asn Gly Leu Gly Val Leu Gly Met Asp Met Thr

130 135 140

Ser Tyr Asp Gln Arg Met Pro Pro Glu Gly Gly Gln Pro Cys Val Val

145 150 155 160

Ala Gln Val Thr Arg Phe Lys Cys Gly Gly Val Val Leu Gly Val Ala

165 170 175

Phe Asn His Thr His Thr Asp Gly Gln Gly Phe Tyr Thr Phe Met Arg

180 185 190

Ala Trp Ser Asp Phe Ser Arg Thr Asn Gly Thr Ala Ile Lys Val Asp

195 200 205

His Asn Arg Ala Leu Pro Glu Leu Ala Ser Leu Ser Gln Phe Phe Ile

210 215 220

Lys Gln His Asp Arg Ile Gly Gly Lys Thr Ser Thr Asp Arg Val Asn

225 230 235 240

Asp His Cys Ser Lys Val Pro Glu Arg Leu Ala Leu Lys Ala Phe Glu

245 250 255

Val Arg Ala Ser Lys Ile Lys Ala Ala Lys Leu Ala Ala Glu Asp Gly

260 265 270

Gly Val Gly Tyr Val Ser Thr Val Asp Cys Ile Val Ala His Leu Trp

275 280 285

Lys Thr Leu Ala Arg Leu Pro Pro Val Val Leu Asp Gly Arg Glu Ile

290 295 300

Thr Val Phe Ser Pro Val Glu Gly Arg Asn Arg Phe Leu Asp Pro Pro

305 310 315 320

Arg Pro Asn Met Cys Gly Asn Cys Phe Ala Ala Met Val Thr Pro Lys

325 330 335

Ile Pro Thr Gln Glu Leu Leu Glu Met Pro Leu Ala Ala Ile Ala Gly

340 345 350

Lys Gln Arg Glu Lys Leu Ser Thr Thr Arg Arg Glu Glu Trp Phe Gly

355 360 365

Gln Gln Ser Phe Arg Glu Leu Ala Ser Ala Met Asn Thr Ser Lys Ser

370 375 380

Ala Leu Leu Ile Val Thr Ser Trp Phe Asn Phe Pro Met Tyr Glu Ile

385 390 395 400

Asp Phe Gly Ala Gly Lys Pro Phe Phe Ala Ser Thr Thr Asn Met Ile

405 410 415

Ser Pro Ile Asn Gly Val Cys Cys Gly Val Ile Ala Pro Pro Thr Pro

420 425 430

Gly Ser Cys Ser Ser Ile Ala Thr Leu Tyr Ile Leu Cys Leu Pro Ala

435 440 445

Val Leu Glu Ala Leu Glu Asn Val Pro Asp Phe Leu Ser Phe Phe Val

450 455 460

Pro His Pro Asn His Lys Asp Asn Ser Gln Thr Gly Pro Phe Ser Phe

465 470 475 480

Val Asp Ile Met

<210> 125

<211> 1530

<212> DNA

<213> 费希尔曲霉(Aspergillus fischeri)_XP_001258079天然核苷酸序列

<400> 125

atgaccgtga ccatcagttt cgagccatat gtgggctcct ctgtcgatgc tctaagcatc 60

cctctctatc ttcgatgtca actcgtcttc aaactttcta agccacttgc tgcggtgcct 120

ctgcttgagt ctggagttaa tcgtcttgta caagcgttac ccttcctctc gggcgagttc 180

acggccgtgc cagcatccga cggtgggaaa gaaattcttc tcgttcgccc tgtgctcaac 240

ttcgagctca gccgtatact caagatcaag taccatgaaa catccctacg acatgtatgc 300

aaacagatga acaggccaag cagccagggt ggtgaccttc cgcatgagcc atacatgccc 360

tacccacgac ttccagatcc ttcacgccct caacccatcg tcgggttcca agtcaacgtt 420

cacacggatg gcatcattct ctccgttgct acgcatcact gttcctttga cgcaacaggg 480

atgggatcaa tcgtccaaaa cctcgcggct tgttgccgtt ctcctccgag cgacgagcct 540

gacttgacca cgtcgccagc ccaggaagca gaagcaagaa aagtcctctc gcaagtccgc 600

gagacgcctt ttgatccaaa gatgttcccg gagtacagac ccttggacag tatgctgtcc 660

tattacaaag gcgtccagtc agcgctccag ggtcgtcaaa ccactatcgt caatcgatgt 720

ttcacaatcg ccgccgacaa gatcaacgcg ctcaaaaggc gctgcaatca actgataccg 780

gaaatggtga agaagtatgg gctttcaact gaggatgcca ttgggagcgc ctgggtctcg 840

agtaatgatg ttgttgctgc cctcttgtgg acatgtatca atcgagcgcg atatcccgaa 900

atccgcgagc gcagcgttca ccagctccca ccagacctcc tacatgcgac atctagcctg 960

ggtgtgccag tgaacgttcg ctctcgactg tcgccgccct tacccaaatc gactttaggt 1020

aacgccgtgt gtcttctccg ggagaaggtc ccgctgcaat ttttcgcttt gcctagtcat 1080

gccaacatgg aggccacttc tagcgtttgc gcagaccatt ccggagacga cgaatgggcc 1140

ttgtccttct gtcgagtcgc ctacggactc agagcgaagc tgaacgcaat tgatgacgac 1200

tatatccgcg actatatctc ctacgtgcaa aagtctccgt gccatctgtc agtgacactg 1260

gatacagaga acctgtacct cagtaactgg cgcgagatcg gtgtgtatga tgctgatttt 1320

ggaggcatgc tgggcaagcc gctacggatg agagctccgg atggatacac cgatggcctg 1380

atttttgtga tggcgcagcg gagcgaagat aagtctgcac cgtgggagtt taatatctcg 1440

ctggaggcat cgacaatgaa gcgtattgtg catgatcccc tctggtgcaa gtatgttgag 1500

ctggatgcgt tctggcatgg agaagaatga 1530

<210> 126

<211> 1530

<212> DNA

<213> 人工_XP_001258079_针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1530)

<400> 126

atg acc gtc acg att tca ttc gaa cca tat gtg gga tct agc gtg gac 48

Met Thr Val Thr Ile Ser Phe Glu Pro Tyr Val Gly Ser Ser Val Asp

1 5 10 15

gcg ctg tcc ata ccc ttg tat cta agg tgc caa ctg gtg ttc aaa tta 96

Ala Leu Ser Ile Pro Leu Tyr Leu Arg Cys Gln Leu Val Phe Lys Leu

20 25 30

tcc aag cct ctt gca gcc gtg ccc ctg cta gaa tct ggg gta aac cgt 144

Ser Lys Pro Leu Ala Ala Val Pro Leu Leu Glu Ser Gly Val Asn Arg

35 40 45

cta gta caa gca tta cca ttc ttg tct ggt gaa ttt act gct gtc cct 192

Leu Val Gln Ala Leu Pro Phe Leu Ser Gly Glu Phe Thr Ala Val Pro

50 55 60

gca agc gat ggt ggg aag gaa atc tta ctt gtt aga cca gtc tta aat 240

Ala Ser Asp Gly Gly Lys Glu Ile Leu Leu Val Arg Pro Val Leu Asn

65 70 75 80

ttc gaa cta agt cgt ata ctg aag atc aaa tac cac gaa aca tcc tta 288

Phe Glu Leu Ser Arg Ile Leu Lys Ile Lys Tyr His Glu Thr Ser Leu

85 90 95

aga cac gta tgc aag cag atg aac aga cca tcc tcg caa ggt ggt gac 336

Arg His Val Cys Lys Gln Met Asn Arg Pro Ser Ser Gln Gly Gly Asp

100 105 110

ttg cca cac gaa ccg tat atg cca tac ccc agg tta cca gat cca tct 384

Leu Pro His Glu Pro Tyr Met Pro Tyr Pro Arg Leu Pro Asp Pro Ser

115 120 125

agg cct caa cca atc gtg ggt ttt caa gtc aat gtc cac act gac ggt 432

Arg Pro Gln Pro Ile Val Gly Phe Gln Val Asn Val His Thr Asp Gly

130 135 140

ata atc ctg agt gta gca act cac cat tgc agt ttt gac gcc acg ggg 480

Ile Ile Leu Ser Val Ala Thr His His Cys Ser Phe Asp Ala Thr Gly

145 150 155 160

atg gga agt att gta cag aac ttg gcc gca tgc tgt aga tct ccg cca 528

Met Gly Ser Ile Val Gln Asn Leu Ala Ala Cys Cys Arg Ser Pro Pro

165 170 175

tcg gac gag cca gat tta act aca agc cct gct caa gaa gca gaa gct 576

Ser Asp Glu Pro Asp Leu Thr Thr Ser Pro Ala Gln Glu Ala Glu Ala

180 185 190

agg aag gtc ctg agc caa gtt aga gaa aca cca ttc gac cca aag atg 624

Arg Lys Val Leu Ser Gln Val Arg Glu Thr Pro Phe Asp Pro Lys Met

195 200 205

ttt ccc gaa tat agg ccc tta gac tct atg tta tct tat tac aaa ggt 672

Phe Pro Glu Tyr Arg Pro Leu Asp Ser Met Leu Ser Tyr Tyr Lys Gly

210 215 220

gtc cag tct gct ttg cag ggt cgt caa act act atc gtt aac aga tgt 720

Val Gln Ser Ala Leu Gln Gly Arg Gln Thr Thr Ile Val Asn Arg Cys

225 230 235 240

ttc act atc gct gct gat aag ata aac gcc tta aag agg aga tgt aac 768

Phe Thr Ile Ala Ala Asp Lys Ile Asn Ala Leu Lys Arg Arg Cys Asn

245 250 255

caa ctt att ccg gaa atg gta aag aaa tac gga ttg agt aca gaa gat 816

Gln Leu Ile Pro Glu Met Val Lys Lys Tyr Gly Leu Ser Thr Glu Asp

260 265 270

gct atc ggt tct gca tgg gtc tct tct aat gac gtg gtt gcc gca ctt 864

Ala Ile Gly Ser Ala Trp Val Ser Ser Asn Asp Val Val Ala Ala Leu

275 280 285

ttg tgg acc tgt att aat cgt gct aga tac cca gag att aga gaa aga 912

Leu Trp Thr Cys Ile Asn Arg Ala Arg Tyr Pro Glu Ile Arg Glu Arg

290 295 300

agt gta cat cag ctt cca cca gac ttg ttg cat gct aca tct tca ttg 960

Ser Val His Gln Leu Pro Pro Asp Leu Leu His Ala Thr Ser Ser Leu

305 310 315 320

ggt gta cct gtt aat gtt aga tct aga ctt tca cca ccc tta ccc aaa 1008

Gly Val Pro Val Asn Val Arg Ser Arg Leu Ser Pro Pro Leu Pro Lys

325 330 335

tcc act ctt ggg aat gcg gtc tgc ctg cta agg gag aag gta cct ttg 1056

Ser Thr Leu Gly Asn Ala Val Cys Leu Leu Arg Glu Lys Val Pro Leu

340 345 350

caa ttc ttc gct ctt ccg agt cac gca aac atg gaa gca acc agt tca 1104

Gln Phe Phe Ala Leu Pro Ser His Ala Asn Met Glu Ala Thr Ser Ser

355 360 365

gtt tgc gct gac cat tca ggg gat gat gaa tgg gcg ttg agc ttt tgt 1152

Val Cys Ala Asp His Ser Gly Asp Asp Glu Trp Ala Leu Ser Phe Cys

370 375 380

agg gta gct tat ggt ctg cgt gca aaa ttg aac gcg atc gat gat gat 1200

Arg Val Ala Tyr Gly Leu Arg Ala Lys Leu Asn Ala Ile Asp Asp Asp

385 390 395 400

tac ata agg gat tac att agt tat gta cag aag tcg ccc tgt cat ctt 1248

Tyr Ile Arg Asp Tyr Ile Ser Tyr Val Gln Lys Ser Pro Cys His Leu

405 410 415

tca gtt aca cta gat act gag aac tta tac ctg tct aat tgg aga gaa 1296

Ser Val Thr Leu Asp Thr Glu Asn Leu Tyr Leu Ser Asn Trp Arg Glu

420 425 430

ata ggc gtc tac gat gca gac ttc ggt ggt atg cta ggg aaa cca ttg 1344

Ile Gly Val Tyr Asp Ala Asp Phe Gly Gly Met Leu Gly Lys Pro Leu

435 440 445

cgt atg aga gct ccg gat ggc tac act gac ggt ttg att ttc gtt atg 1392

Arg Met Arg Ala Pro Asp Gly Tyr Thr Asp Gly Leu Ile Phe Val Met

450 455 460

gcc caa aga tct gaa gac aag tca gct ccg tgg gaa ttc aac ata tcc 1440

Ala Gln Arg Ser Glu Asp Lys Ser Ala Pro Trp Glu Phe Asn Ile Ser

465 470 475 480

ctt gag gct tct aca atg aaa agg att gta cat gat ccg ctg tgg tgt 1488

Leu Glu Ala Ser Thr Met Lys Arg Ile Val His Asp Pro Leu Trp Cys

485 490 495

aaa tat gtt gaa ttg gat gcc ttt tgg cat ggg gaa gag taa 1530

Lys Tyr Val Glu Leu Asp Ala Phe Trp His Gly Glu Glu

500 505

<210> 127

<211> 509

<212> PRT

<213> 人工_XP_001258079_针对其在酿酒酵母中的表达经密码子优化

<400> 127

Met Thr Val Thr Ile Ser Phe Glu Pro Tyr Val Gly Ser Ser Val Asp

1 5 10 15

Ala Leu Ser Ile Pro Leu Tyr Leu Arg Cys Gln Leu Val Phe Lys Leu

20 25 30

Ser Lys Pro Leu Ala Ala Val Pro Leu Leu Glu Ser Gly Val Asn Arg

35 40 45

Leu Val Gln Ala Leu Pro Phe Leu Ser Gly Glu Phe Thr Ala Val Pro

50 55 60

Ala Ser Asp Gly Gly Lys Glu Ile Leu Leu Val Arg Pro Val Leu Asn

65 70 75 80

Phe Glu Leu Ser Arg Ile Leu Lys Ile Lys Tyr His Glu Thr Ser Leu

85 90 95

Arg His Val Cys Lys Gln Met Asn Arg Pro Ser Ser Gln Gly Gly Asp

100 105 110

Leu Pro His Glu Pro Tyr Met Pro Tyr Pro Arg Leu Pro Asp Pro Ser

115 120 125

Arg Pro Gln Pro Ile Val Gly Phe Gln Val Asn Val His Thr Asp Gly

130 135 140

Ile Ile Leu Ser Val Ala Thr His His Cys Ser Phe Asp Ala Thr Gly

145 150 155 160

Met Gly Ser Ile Val Gln Asn Leu Ala Ala Cys Cys Arg Ser Pro Pro

165 170 175

Ser Asp Glu Pro Asp Leu Thr Thr Ser Pro Ala Gln Glu Ala Glu Ala

180 185 190

Arg Lys Val Leu Ser Gln Val Arg Glu Thr Pro Phe Asp Pro Lys Met

195 200 205

Phe Pro Glu Tyr Arg Pro Leu Asp Ser Met Leu Ser Tyr Tyr Lys Gly

210 215 220

Val Gln Ser Ala Leu Gln Gly Arg Gln Thr Thr Ile Val Asn Arg Cys

225 230 235 240

Phe Thr Ile Ala Ala Asp Lys Ile Asn Ala Leu Lys Arg Arg Cys Asn

245 250 255

Gln Leu Ile Pro Glu Met Val Lys Lys Tyr Gly Leu Ser Thr Glu Asp

260 265 270

Ala Ile Gly Ser Ala Trp Val Ser Ser Asn Asp Val Val Ala Ala Leu

275 280 285

Leu Trp Thr Cys Ile Asn Arg Ala Arg Tyr Pro Glu Ile Arg Glu Arg

290 295 300

Ser Val His Gln Leu Pro Pro Asp Leu Leu His Ala Thr Ser Ser Leu

305 310 315 320

Gly Val Pro Val Asn Val Arg Ser Arg Leu Ser Pro Pro Leu Pro Lys

325 330 335

Ser Thr Leu Gly Asn Ala Val Cys Leu Leu Arg Glu Lys Val Pro Leu

340 345 350

Gln Phe Phe Ala Leu Pro Ser His Ala Asn Met Glu Ala Thr Ser Ser

355 360 365

Val Cys Ala Asp His Ser Gly Asp Asp Glu Trp Ala Leu Ser Phe Cys

370 375 380

Arg Val Ala Tyr Gly Leu Arg Ala Lys Leu Asn Ala Ile Asp Asp Asp

385 390 395 400

Tyr Ile Arg Asp Tyr Ile Ser Tyr Val Gln Lys Ser Pro Cys His Leu

405 410 415

Ser Val Thr Leu Asp Thr Glu Asn Leu Tyr Leu Ser Asn Trp Arg Glu

420 425 430

Ile Gly Val Tyr Asp Ala Asp Phe Gly Gly Met Leu Gly Lys Pro Leu

435 440 445

Arg Met Arg Ala Pro Asp Gly Tyr Thr Asp Gly Leu Ile Phe Val Met

450 455 460

Ala Gln Arg Ser Glu Asp Lys Ser Ala Pro Trp Glu Phe Asn Ile Ser

465 470 475 480

Leu Glu Ala Ser Thr Met Lys Arg Ile Val His Asp Pro Leu Trp Cys

485 490 495

Lys Tyr Val Glu Leu Asp Ala Phe Trp His Gly Glu Glu

500 505

<210> 128

<211> 1323

<212> DNA

<213> 土曲霉(Aspergillus terreus) _XP_001217250天然核苷酸序列

<400> 128

atggcaacct tcgaccatat cgaggatgtc atcggccagc tacctatgct gaagagctac 60

acccatatct tgctgtgctt tcccctcgct gagagccaac tcaatgaagc catcgagagc 120

cttgaatctg ctgtacgtca ggttataaaa accttttcgt tcttggctgg caaagtagtc 180

aacgaaggca agggacccaa cagctcgggt actttcaggg tcgctccctg tgagacgtgg 240

gagtccccag atcatcaatt tgtgcgagtc gttgatcgct ctttcatgct ggcctcctac 300

gatgagatcc gcggagcaca ggcacctgct tccatgctcc caggaagtca actcgggtat 360

cgggtggctt ttccagcgca ttatcacgag acagaggacg atcccgcgcc ggtcctggac 420

attcagtgca atttgatacg gggcggactg ctacttgaca tcgctgccca acacaacatt 480

atcgatgcga gcggtatctt tcaaattgcc agcttgatcg ctctttccat gcgaggcgag 540

tcaattcctg aggatgtcat caaggaaggg aaccgtgatc gacgcaatat cattccacta 600

ctagaggcag atgagcctct tcttgatcac agcgagctca aagccagcag cgcggtgcaa 660

aatccgccgc ccgtcaactt cctccagggg tataaatggc aaatcttcaa gctctctgcc 720

gaggtgttaa ctcgaattac cgctgaagga cgacgacagc cacaggagtt tgtcccctcc 780

gtcacatttg tctcggcaaa cgattgcttg acagcctttc tgtggcaacg ggtgatagcc 840

atgcgcctga agcggctcca tacgcccgag gccgtatcca aactgagccg tgctgttgat 900

cttcggcggg cgatgggcat taccccagca tacatgggcc atatgattcg tgtcgcaaat 960

actagtctca cttttcaaga aattgtggca tgctctttat ccaggcttgc atctctgctc 1020

cgcaagagca tcatcgatgt cagccagccg tatgcgattc ggagctacgt gacctttatt 1080

gcaaatgaga cggataaatc aaagattgcg tatgcgggtg ctttcaatcc ttgcaccgac 1140

atgtcgtgct cgtccattgc acacatcact gctcctgaat tcggtcgttt gggagcgcct 1200

gactttataa ggaggcctac ctacgggcct ctgccgtgct gcacctatgt cgctcccgat 1260

aagaacgatg gagctttgga tcaaaaccag gcatggtcag acgttgtgaa gcgcatcggt 1320

tga 1323

<210> 129

<211> 1323

<212> DNA

<213> 人工_XP_001217250_针对其在酿酒酵母中的表达经密码子优化

<220>

<221> CDS

<222> (1)..(1323)

<400> 129

atg gcc acc ttc gac cac att gag gac gtg ata gga caa ttg ccc atg 48

Met Ala Thr Phe Asp His Ile Glu Asp Val Ile Gly Gln Leu Pro Met

1 5 10 15

cta aag agc tac aca cat att tta ctt tgc ttt ccg ttg gca gaa tct 96

Leu Lys Ser Tyr Thr His Ile Leu Leu Cys Phe Pro Leu Ala Glu Ser

20 25 30

caa ctg aac gag gcg att gaa tct ttg gag tca gca gtc aga caa gta 144

Gln Leu Asn Glu Ala Ile Glu Ser Leu Glu Ser Ala Val Arg Gln Val

35 40 45

att aag acg ttt tca ttc ctt gca ggt aag gtt gtt aat gaa ggc aaa 192

Ile Lys Thr Phe Ser Phe Leu Ala Gly Lys Val Val Asn Glu Gly Lys

50 55 60

ggt ccg aat tcc tct ggt acg ttc cgt gtt gct ccg tgt gaa act tgg 240

Gly Pro Asn Ser Ser Gly Thr Phe Arg Val Ala Pro Cys Glu Thr Trp

65 70 75 80

gaa tct cct gat cat caa ttt gtt cgt gtt gta gat agg agc ttt atg 288

Glu Ser Pro Asp His Gln Phe Val Arg Val Val Asp Arg Ser Phe Met

85 90 95

ttg gcc tcg tat gat gag atc cgt ggt gct caa gcc ccc gct tcc atg 336

Leu Ala Ser Tyr Asp Glu Ile Arg Gly Ala Gln Ala Pro Ala Ser Met

100 105 110

tta cct ggg tca caa ctg ggg tat agg gta gca ttc cca gcc cat tat 384

Leu Pro Gly Ser Gln Leu Gly Tyr Arg Val Ala Phe Pro Ala His Tyr

115 120 125

cat gaa act gaa gac gat cca gct cca gtt cta gat atc cag tgt aat 432

His Glu Thr Glu Asp Asp Pro Ala Pro Val Leu Asp Ile Gln Cys Asn

130 135 140

ttg ata aga ggt ggt ctg ctt cta gat ata gcg gcc caa cac aac ata 480

Leu Ile Arg Gly Gly Leu Leu Leu Asp Ile Ala Ala Gln His Asn Ile

145 150 155 160

att gat gct tcc ggg ata ttt caa atc gct agt ctg atc gcc tta agc 528

Ile Asp Ala Ser Gly Ile Phe Gln Ile Ala Ser Leu Ile Ala Leu Ser

165 170 175

atg aga gga gaa agt atc cct gaa gat gtt atc aaa gaa gga aac aga 576

Met Arg Gly Glu Ser Ile Pro Glu Asp Val Ile Lys Glu Gly Asn Arg

180 185 190

gat aga agg aat atc att ccg tta ttg gaa gcc gat gag cct tta tta 624

Asp Arg Arg Asn Ile Ile Pro Leu Leu Glu Ala Asp Glu Pro Leu Leu

195 200 205

gac cat agt gaa ttg aag gca tcc agc gcc gtt cag aac ccg cca cca 672

Asp His Ser Glu Leu Lys Ala Ser Ser Ala Val Gln Asn Pro Pro Pro

210 215 220

gtt aat ttc ttg caa ggt tat aaa tgg cag att ttc aaa ctg tcc gct 720

Val Asn Phe Leu Gln Gly Tyr Lys Trp Gln Ile Phe Lys Leu Ser Ala

225 230 235 240

gag gta ttg acc cgt att act gcc gaa gga cgt aga caa cca caa gaa 768

Glu Val Leu Thr Arg Ile Thr Ala Glu Gly Arg Arg Gln Pro Gln Glu

245 250 255

ttt gtg ccc tca gtg acc ttt gtg tcc gca aac gac tgc ttg act gct 816

Phe Val Pro Ser Val Thr Phe Val Ser Ala Asn Asp Cys Leu Thr Ala

260 265 270

ttc ttg tgg cag cgt gtg ata gct atg agg cta aag aga ttg cat acc 864

Phe Leu Trp Gln Arg Val Ile Ala Met Arg Leu Lys Arg Leu His Thr

275 280 285

ccc gag gcc gtt tcc aag cta tct aga gcc gtg gat tta aga agg gcc 912

Pro Glu Ala Val Ser Lys Leu Ser Arg Ala Val Asp Leu Arg Arg Ala

290 295 300

atg ggt ata acc cct gca tac atg ggc cat atg ata aga gtc gca aac 960

Met Gly Ile Thr Pro Ala Tyr Met Gly His Met Ile Arg Val Ala Asn

305 310 315 320

aca tcc tta acc ttt caa gaa att gtc gca tgt agt tta tca cgt ctt 1008

Thr Ser Leu Thr Phe Gln Glu Ile Val Ala Cys Ser Leu Ser Arg Leu

325 330 335

gcg agc tta ctt aga aaa tct atc att gat gtg tcc caa cca tat gcg 1056

Ala Ser Leu Leu Arg Lys Ser Ile Ile Asp Val Ser Gln Pro Tyr Ala

340 345 350

atc aga tca tac gtc acc ttc ata gca aac gaa aca gac aaa tct aag 1104

Ile Arg Ser Tyr Val Thr Phe Ile Ala Asn Glu Thr Asp Lys Ser Lys

355 360 365

ata gcc tat gcc ggg gca ttt aac ccg tgt act gat atg tca tgt tca 1152

Ile Ala Tyr Ala Gly Ala Phe Asn Pro Cys Thr Asp Met Ser Cys Ser

370 375 380

tcc ata gct cat att acg gca ccg gag ttt ggt agg tta ggg gcg cct 1200

Ser Ile Ala His Ile Thr Ala Pro Glu Phe Gly Arg Leu Gly Ala Pro

385 390 395 400

gac ttc att agg aga ccc act tat ggc cca cta cct tgc tgt act tac 1248

Asp Phe Ile Arg Arg Pro Thr Tyr Gly Pro Leu Pro Cys Cys Thr Tyr

405 410 415

gta gct cct gac aag aat gac ggt gca ttg gat cag aat caa gca tgg 1296

Val Ala Pro Asp Lys Asn Asp Gly Ala Leu Asp Gln Asn Gln Ala Trp

420 425 430

tca gat gtc gta aag agg ata ggt taa 1323

Ser Asp Val Val Lys Arg Ile Gly

435 440

<210> 130

<211> 440

<212> PRT

<213> 人工_XP_001217250_针对其在酿酒酵母中的表达经密码子优化

<400> 130

Met Ala Thr Phe Asp His Ile Glu Asp Val Ile Gly Gln Leu Pro Met

1 5 10 15

Leu Lys Ser Tyr Thr His Ile Leu Leu Cys Phe Pro Leu Ala Glu Ser

20 25 30

Gln Leu Asn Glu Ala Ile Glu Ser Leu Glu Ser Ala Val Arg Gln Val

35 40 45

Ile Lys Thr Phe Ser Phe Leu Ala Gly Lys Val Val Asn Glu Gly Lys

50 55 60

Gly Pro Asn Ser Ser Gly Thr Phe Arg Val Ala Pro Cys Glu Thr Trp

65 70 75 80

Glu Ser Pro Asp His Gln Phe Val Arg Val Val Asp Arg Ser Phe Met

85 90 95

Leu Ala Ser Tyr Asp Glu Ile Arg Gly Ala Gln Ala Pro Ala Ser Met

100 105 110

Leu Pro Gly Ser Gln Leu Gly Tyr Arg Val Ala Phe Pro Ala His Tyr

115 120 125

His Glu Thr Glu Asp Asp Pro Ala Pro Val Leu Asp Ile Gln Cys Asn

130 135 140

Leu Ile Arg Gly Gly Leu Leu Leu Asp Ile Ala Ala Gln His Asn Ile

145 150 155 160

Ile Asp Ala Ser Gly Ile Phe Gln Ile Ala Ser Leu Ile Ala Leu Ser

165 170 175

Met Arg Gly Glu Ser Ile Pro Glu Asp Val Ile Lys Glu Gly Asn Arg

180 185 190

Asp Arg Arg Asn Ile Ile Pro Leu Leu Glu Ala Asp Glu Pro Leu Leu

195 200 205

Asp His Ser Glu Leu Lys Ala Ser Ser Ala Val Gln Asn Pro Pro Pro

210 215 220

Val Asn Phe Leu Gln Gly Tyr Lys Trp Gln Ile Phe Lys Leu Ser Ala

225 230 235 240

Glu Val Leu Thr Arg Ile Thr Ala Glu Gly Arg Arg Gln Pro Gln Glu

245 250 255

Phe Val Pro Ser Val Thr Phe Val Ser Ala Asn Asp Cys Leu Thr Ala

260 265 270

Phe Leu Trp Gln Arg Val Ile Ala Met Arg Leu Lys Arg Leu His Thr

275 280 285

Pro Glu Ala Val Ser Lys Leu Ser Arg Ala Val Asp Leu Arg Arg Ala

290 295 300

Met Gly Ile Thr Pro Ala Tyr Met Gly His Met Ile Arg Val Ala Asn

305 310 315 320

Thr Ser Leu Thr Phe Gln Glu Ile Val Ala Cys Ser Leu Ser Arg Leu

325 330 335

Ala Ser Leu Leu Arg Lys Ser Ile Ile Asp Val Ser Gln Pro Tyr Ala

340 345 350

Ile Arg Ser Tyr Val Thr Phe Ile Ala Asn Glu Thr Asp Lys Ser Lys

355 360 365

Ile Ala Tyr Ala Gly Ala Phe Asn Pro Cys Thr Asp Met Ser Cys Ser

370 375 380

Ser Ile Ala His Ile Thr Ala Pro Glu Phe Gly Arg Leu Gly Ala Pro

385 390 395 400

Asp Phe Ile Arg Arg Pro Thr Tyr Gly Pro Leu Pro Cys Cys Thr Tyr

405 410 415

Val Ala Pro Asp Lys Asn Asp Gly Ala Leu Asp Gln Asn Gln Ala Trp

420 425 430