新型Cas13蛋白及其筛选方法和应用

相关申请

本申请要求于2021年6月4日提交的标题为“新型Cas13蛋白及其筛选方法和应用”的申请号为的申请的优先权，其全部内容通过引用并入本文。

技术领域

本公开内容涉及生物技术及医学领域。更具体地，本公开内容涉及新的Cas13家族蛋白、筛选新的Cas13家族蛋白的方法、以及相应的RNA编辑系统及其应用。本公开内容尤其涉及Cas13蛋白及相关的RNA编辑系统。

背景技术

CRISPR-Cas系统是原核生物(主要是细菌和古细菌)的一种适应性免疫系统，能够对外源病毒(例如噬菌体)的入侵进行防御。依据作用对像不同可以分为两大类，一类是靶向DNA的CRISPR-Cas系统，例如CRISPR-Cas9；另一类则是靶向RNA的CRISPR-Cas系统，例如Cas13家族，包括Cas13a、Cas13b、Cas13c、Cas13d、Cas13X、Cas13Y，等。与DNA编辑的CRISPR-Cas系统相比，CRISPR-Cas13系统主要由HEPN(higher eukaryotes and prokaryotesnucleotide)结构域来发挥切割和抵御外来入侵核酸，因而靶向RNA的CRISPR-Cas的作用更加温和且直接，能够在不改变基因组的情况下进行RNA转录本的调控，提高了基因编辑的安全性。然而当前已有发现的CRISPR-Cas系统的效应蛋白分子量都很大(>900个氨基酸)，多数都是需要多Cas蛋白组合才能发挥功能，而分子量太大不利于慢病毒和腺相关病毒(adeno-associated virus，AAV)的包装，极大了限制了CRISPR-Cas蛋白在真核生物(尤其是人源细胞)中的转染应用。因此，寻找分子量更低且特异性更强的新型CRISPR-Cas蛋白仍是本领域中的重要任务。

发明内容

针对现有筛选新型CRISPR-Cas蛋白技术的不足和实际需求，本公开内容提供了一种快速寻找分子量较低(氨基酸数目为300到950)并且包含较多的拓展的HEPN结构域(至少2个)的新型CRISPR-Cas13直系同源蛋白的方法并从生物信息分析层面(例如，序列比对、蛋白结构预测等)和实验层面验证了候选蛋白的RNA编辑活性。这些蛋白潜在应用于RNA层面的调控、编辑、检测等方面，具有广阔的学术价值和商业应用价值。

本公开内容所解决的技术问题是如何快速寻找新型的分子量较低且RNA酶切活性结构域(拓展的HEPN结构域)较多的候选CRISPR-Cas13蛋白及其系统；其次是验证候选CRISPR-Cas13蛋白及其系统的活性；并最终获得了多种新型Cas13蛋白。

本公开内容实现了以下技术效果：

(1)开发了快速筛选新型低分子量的Cas13家族蛋白的分析方法，该方法可以对新更新的原核微生物DNA序列和宏基因组序列进行CRIPSR array系统的分析和相关效应蛋白的筛选；

(2)筛选的低分子量Cas13家族成员，拓展CRISPR-Cas13的应用范围，能够更容易的被腺相关病毒等递送载体包装从而进行相关疾病诊疗，如神经相关退行性疾病的诊疗，在植物领域则可以开展育种，逆境胁迫等方面的研究，在微生物领域可以进行相关工程菌的改造等；

(3)低分子量的Cas13蛋白对于人工合成而言能够进一步节省成本；

(4)本方法在筛选过程中，除利用Cas13蛋白的已知HEPN结构域进行筛选外，还将其他种类的蛋白质中具备RNA切割活性的保守型结构域包括在内，从而提供了筛选新的Cas13蛋白的可能，并且由于这些新Cas13蛋白中这些新的功能结构域的鉴定，为进一步改造Cas13蛋白提供了新的思路和可能性。

在本公开内容的一个方面中，提供了一种鉴定新的功能性Cas13蛋白的方法，其包括：

a)CRISPR array区域的鉴定，其包括从细菌、古细菌基因组以及宏基因组序列中鉴定CRISPR array区域；

b)在所鉴定的CRISPR array区域附近搜寻Cas13候选蛋白，其包括在所鉴定区域的上游和下游检索包含目标结构域的蛋白质，所述目标结构域为HEPN结构域和其他具有RNA切割活性的结构域；

c)对检索到的蛋白质进行氨基酸(AA)长度分析，选取包含至少2个目标结构域并且长度小于950个氨基酸的Cas13候选蛋白；

d)从c)得到的结果中除去功能已知的蛋白质；以及

e)对候选蛋白进行功能验证，筛选出功能性Cas13蛋白。

在一个优选的实施方案中，所述目标结构域为氨基酸序列RX

在一个优选的实施方案中，所述HEPN结构域来自于表3中的序列。

在一个优选的实施方案中，所述其他具有RNA切割活性的结构域来自表2中的序列。

在一个优选的实施方案中，在步骤b)中，对所鉴定的CRISPR array区域上下游距离最近的各6个ORF进行目标结构域检索。

在一个优选的实施方案中，所述功能验证包括验证对RNA的切割活性，所述Cas13蛋白能够与所在所属物种基因组的CRISPR array上的同向重复(DR)序列和间隔区域结合形成复合物而发挥RNA切割作用

在本公开内容的另一个方面中，提供了通过上述方法筛选到的Cas13蛋白。

在一个优选的实施方案中，所述Cas13蛋白包含如SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列，或具有一个或更多个残基的保守氨基酸取代的SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24和25中任一项所述的氨基酸序列。

在一个优选的实施方案中，所述Cas13蛋白的RNA切割活性被保留。

在一个优选的实施方案中，所述Cas13蛋白的HEPN结构域或RNA切割结构域经进一步修饰或改造，而使其RNA切割活性降低或消除，成为RNA切割活性降低或消除的dCas13。

在一个优选的实施方案中，所述Cas13蛋白与一个或更多个异源功能性结构域融合。

在一个优选的实施方案中，所述融合在所述Cas13蛋白的N端、C端或者内部。

在一个优选的实施方案中，所述一个或更多个异源功能性结构域具有以下活性：脱氨酶如胞苷脱氨基酶和脱氧腺苷脱氨基酶、甲基化酶、去甲基化酶、转录激活、转录抑制、核酸酶、单链RNA裂解、双链RNA裂解、单链DNA裂解、双链DNA裂解、DNA或RNA连接酶、报告蛋白、检测蛋白、定位信号、或其任意组合。在本公开内容的另一个方面中，提供了一种核酸分子，其包含编码上述Cas13蛋白的核苷酸序列。

在一个优选的实施方案中，所述核酸分子针对在特定宿主细胞中的表达而进行了密码子优化。

在一个优选的实施方案中，所述宿主细胞是真核生物细胞，优选人细胞。

在一个优选的实施方案中，所述核酸分子包含与编码Cas13的核苷酸序列有效链接的启动子，其为组成型启动子、诱导型启动子、组织特异性启动子或发育特异性启动子。

在本公开内容的另一个方面中，提供了一种表达载体，其包含上述核酸分子。

在一个优选的实施方案中，所述表达载体为腺相关病毒(AAV)、腺病毒、慢病毒、逆转录病毒、单纯孢疹病毒、溶瘤病毒。

在本公开内容的另一个方面中，提供了一种递送系统，其包含(1)上述表达载体，或上述Cas13蛋白；以及(2)递送载体。

在一个优选的实施方案中，所述递送载体是纳米颗粒、脂质体、外泌体、微囊泡或基因枪。

在本公开内容的另一个方面中，提供了一种CRISPR-Cas系统，其包含：(1)上述Cas13蛋白或核酸分子，或者其衍生物或功能片段；(2)用于靶向目标RNA的gRNA序列。

在一个优选的实施方案中，其中所述gRNA序列包含同向重复(DR)序列和靶向靶RNA部分的间隔区域的序列。

在一个优选的实施方案中，其中所述DR序列为表4中所示序列；其中所述间隔区序列为15-60个核苷酸，优选25-50个核苷酸，更优选30个核苷酸。

在一个优选的实施方案中，所述DR序列可以是对应以下任一项的衍生物，其中所述衍生物(i)与表4中所示序列中的任一个相比，具有一个或多个(例如1、2、3、4、5、6、7、8、9或10)个核苷酸的添加、缺失、或取代；(ii)与表4中所示序列中任何一个具有至少20％、30％、40％、50％、60％、70％、80％、90％、95％或97％的序列同一性；(iii)在严格条件下与表4中所示序列任意一个，或与(i)和(ii)中的任意一个杂交；或(iv)是(i)-(iii)中任何一个的互补物，条件是所述衍生物非表4中所示序列中的任何一个，并且所述衍生物编码一个RNA，或本身即是一个RNA，所述RNA与SEQ ID NO：75-105编码的任意RNA基本保持相同的二级结构。

在一个优选的实施方案中，所述CRISPR-Cas系统还包含：(3)靶RNA。

在一个优选的实施方案中，所述CRISPR-Cas系统引起靶RNA序列的降解、切割或序列的改变。

在一个优选的实施方案中，所述靶RNA是mRNA或ncRNA，包括选自lncRNA、miRNA、misc_RNA、Mt_rRNA、Mt_tRNA、rRNA、scaRNA、scRNA、snoRNA、snRNA、sRNA的非编码RNA。

在本公开内容的另一个方面中，提供了一种细胞，其包含上述Cas13蛋白、核酸分子、表达载体、递送系统或CRISPR-Cas系统。

在一个优选的实施方案中，所述细胞为原核细胞或真核细胞，优选人细胞。

在本公开内容的另一个方面中，提供了一种降解或切割目的细胞中靶RNA、修饰目的细胞中靶RNA的序列的方法，其包括使用上述Cas13蛋白、核酸分子、表达载体、递送载体或CRISPR-Cas系统。

在一个优选的实施方案中，所述目的细胞为原核细胞或真核细胞，优选人细胞。

在一个优选的实施方案中，其中所述目的细胞为离体细胞、体外细胞或体内细胞。

附图说明

图1是从头寻找CRISPR-Cas系统低分子量蛋白流程图。

图2是体外细胞试验验证候选蛋白活性策略。

图3-11示出了随机对候选蛋白在细胞层面上的验证的结果。

图3示出了在哺乳动物细胞系中检测Cas13d[xdz9](阳性对照)酶切活性的实验结果：上图为含有cas13d蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组px261为实验组。

图4示出了在哺乳动物细胞系中检测候选蛋白DZ102酶切活性的实验结果：图4A为含有DZ102蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP487、AP489、AP491为实验组。图4B为共转染48h后进行流式分析的红光mcherry的knock down(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg4，其中sg1,sg3,sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ102潜在具有Rnase活性。

图5A示出了在哺乳动物细胞系检中测候选蛋白DZ78酶切活性实验结果：上图为含有DZ78蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP671、HP28、HP25为实验组。

图5B示出了在哺乳动物细胞系中检测候选蛋白DZ78酶切活性的实验结果：上图为含有DZ78蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系48h后进行流式分析细胞的结果，其中横坐标是指示绿色荧光；纵坐标表示红色荧光；px262为阴性对照组，AP671、HP28、HP25为实验组。

图6示出了在哺乳动物细胞系中检测候选蛋白DZ41酶切活性的实验结果：上图为含有DZ41蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP599、AP593为实验组。

图7示出了在哺乳动物细胞系检测候选蛋白DZ77酶切活性实验结果：图7A为含有DZ77蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP659为实验组。图7B则表示共转染48h后进行流式分析的红光mcherry的knockdown(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg5，其中sg2，sg3，sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ77潜在具有Rnase活性。

图8示出了在哺乳动物细胞系中检测候选蛋白DZ79酶切活性的实验结果：上图为含有DZ79蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP677为实验组。

图9示出了在哺乳动物细胞系中检测候选蛋白DZ81酶切活性的实验结果：图9A为含有DZ81蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP695为实验组。图9B则表示共转染48h后进行流式分析的红光mcherry的knock down(KD)的红光信号值，靶向mCherry不同位置的guide RNA即为sg，包括编号sg1～sg5，其中sg1，sg3，sg4都显示了一定程度的KD mcherry的效果，说明候选蛋白DZ81潜在具有Rnase活性。

图10示出了在哺乳动物细胞系中检测候选蛋白DZ82酶切活性的实验结果：上图为含有DZ82蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图；px262为阴性对照组，AP699、AP701为实验组。

图11展示了候选蛋白在293T细胞系上验证其Knock down mCherry的验证实验，其中图11A示出了在哺乳动物细胞系中检测候选蛋白DZ75、DZ76、DZ87、DZ89、DZ95、DZ103和DZ104的酶切活性的实验结果(实验组)：上图为含有DZ75、DZ76、DZ87、DZ89、DZ95、DZ103和DZ104蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中white light表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图。图11B显示了候选蛋白DZ87，DZ89，DZ95，DZ92，DZ97，DZ99，DZ101切割mCherry的流式分析结果，可以看到候选蛋白在与靶向mCherry的不同位置的sgRNA质粒共转染293T细胞系后48h的流式分析检测均表明它们具有一定程度的Rnase活性，效率不是很高，这可能与候选蛋白的潜在PFS有关。

图12示出了在哺乳动物细胞系中检测候选蛋白DZ89、DZ95和cas13d酶切活性的实验结果(对照组)：上图阴性对照为只含有DZ89、DZ95以及cas13d蛋白的质粒(不含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。而阳性对照为cas13d蛋白的质粒(含有对应靶向mCherry的sgRNA)与含有mCherry蛋白的质粒共转染293T细胞系24h后，10倍镜下观察到的荧光结果图。其中whitelight表示白光视野下的细胞结果；Green light表示绿色荧光下的结果图；Red light表示红色荧光下的结果图。

图13展示的是候选蛋白DZ77，DZ78，DZ81，DZ87，DZ89，DZ92，DZ95，DZ97，DZ99，DZ101，DZ102等蛋白的knock down mcherry红光的流式分析结果，从mCherry上随机筛选的3个sgRNA靶向spacer来看，候选蛋白DZ78的KD效果最好，其他蛋白则可能与其强PFS有关。

图14示出了DZ78候选蛋白切割内源基因的实验结果。图14A示出利用DZ78候选蛋白切割内源基因Ptbp1的切割效果，其中hP4、hP5、hP6为实验组不同sgRNA靶向内源基因Ptbp1的不同区域，而hP19则为阴性对照。可以发现与阴性对照相比，候选蛋白DZ78能够切割内源基因Ptbp1，而且hP4效果最好。图14B示出利用DZ78候选蛋白切割内源基因Rest的切割效果，其中hRT1、hRT2、hRT3为实验组不同sgRNA靶向内源基因Rest的不同区域，而hRT19则为阴性对照。可以发现与阴性对照相比，候选蛋白DZ78能够很好的切割内源基因Rest，而且hRT1效果最好。图14C则展示候选蛋白DZ78切割其他内源基因的knock down结果，涉及多个内源基因，包括EZH2，HRAS，EGFR，KRAS，NF2，NFKB1，NRAS，PPARG，RAF1，SMARCA4，STAT3。这些结果表明DZ78具有广泛切割RNA的能力。

图15示出了候选蛋白Direct repeat序列的RNA二级结构。图中展示的是候选蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82、DZ85和DZ102蛋白的DR二级结构。

图16示出了Cas蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102的拓展的HEPN位置模式图。

图17示出了候选蛋白DZ41、DZ77、DZ78、DZ79、DZ80、DZ82和DZ102的基因组模式图。

图18A展示候选蛋白DZ78的潜在PFS的验证实验：通过针对spacer前后设计4个连续相同碱基的sgRNA，然后进行靶向mCherry进行分别与候选蛋白共转染，转染48h后进行流式分选检测，可以发现候选蛋白DZ78在连续4碱基模式下，并没有显示很强的PFS偏好性，提示我们的候选蛋白DZ78潜在能靶向RNA更加任意的区域，具有更广泛的应用价值。

图18B展示了候选蛋白DZ78的sgRNA成熟的机制：为了验证候选蛋白引导RNA的成熟机制，我们设计4种sgRNA：包括DR-sg-DR(即为double DR)，DR-sg(即为5’方向DR)，sg-DR(即为3’方向DR)以及非靶向目标RNA的control sgRNA。同时为了验证其在细胞核内和细胞核外的Rnase活性，还对sgRNA进行添加入核信号(NLS)。从结果可以发现，不论是细胞核内还是细胞核外，候选蛋白DZ78只有在于双DR或者3’方向DR共转染时才能发挥其Rnase活性，表明候选蛋白DZ78的guide RNA成熟体系为3’方向。

图18C和图18D展示了候选蛋白DZ78的guide RNA长度对其Rnase活性的影响。通过设计共转染质粒(如图18C所示)，可以看到不同spacer长度对giude RNA引导候选蛋白DZ78靶向切割mCherry的影响很大(如图18D所示)，其中当spacer长度低于25bp的时候效率降低很多，而30bp～50bp间差异不是很大。

具体实施方式

下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于举例说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。

如在说明书中所使用的，没有数量词修饰的名词可意指一个/种或更多个/种。如在权利要求书中所使用的，当与词语“包含/包括”结合使用时，没有数量词修饰的名词可意指一个/种或多于一个/种。

权利要求书中术语“或/或者”的使用用于意指“和/或”，除非明确地指出仅指替代方案或替代方案是相互排斥的，尽管本公开内容支持仅指替代方案和“和/或”的限定。如本文中使用的“另一/另一些”可意指至少第二或更多个/种。

在整个本申请中，术语“约”用于表示值包括装置的误差、用于确定该值的方法的固有变化，或者存在于研究对象之间的固有变化。这样的固有变异可以是标注值的±10％的变异。

在整个申请中，除非另有说明，否则核苷酸序列以5’至3’方向列出，并且氨基酸序列以N端至C端方向列出。

通过以下详细描述，本发明的其他目的、特征和优点将变得明显。然而，应理解，尽管表明了本发明的一些优选实施方案，但是详细描述和具体实施例仅以举例说明的方式给出，因为根据该详细描述，在本发明的精神和范围内的多种变化和修改对于本领域技术人员而言将变得明显。

NCBI(https://www.ncbi.nlm.nih.gov/)是指美国国家生物信息中心，是一个面向全世界的公共数据库，本领域技术人员利用该数据库提供的核酸数据库进行下载原核生物的基因组，蛋白质组相关数据库等，也可以利用该数据提供的blast比对软件进行序列比对的分析。

IMG(https://img.jgi.doe.gov/)是指微生物基因组整合数据库，是新一代基因组数据库的代表，不仅能够完整收录现有数据库的内容，还提供了更完善的数据上传、注释和分析服务，将测序数据储存到IMG/M数据库。该数据可以下载纯培养细菌测序基因组、宏基因组、宏基因组组装基因组、单细胞测序基因组的数据。

CRISPR(cluster regularly interspaced short palindromic repeats)是原核生物，主要是指细菌和古细菌体内的一串DNA序列，包括DR区域和spacer区域。而CRIPSR系统除了包含CRISPR array外，还包括相关的Cas蛋白。它们一起构成了细菌低于外来病毒入侵的免疫系统。

Cas13家族是目前已知该能够靶向RNA的CRIPSR酶家族，其成员包括Cas13a、Cas13b、Cas13c、Cas13d、Cas13X和Cas13Y家族。与CRISPR/Cas9切割DNA的活性不同，CRISPR/Cas13能够用于切割细菌细胞中特定的RNA序列。

HEPN结构域是higher eukaryotes and prokaryotes nucleotide domain的简称，是CRISPR-Cas13酶系统中Cas13蛋白发挥切割和抵御外来入侵核酸的重要结构域。

ABE系统是Adenine base editors的简称，即嘌呤碱基转换技术，能够实现A/T到G/C的单碱基改变。最常用的酶是adar酶(adenosine deaminases acting on RNA，一种作用于RNA的腺苷脱氨酶)。主要是通过将腺嘌呤脱氨基成肌苷，在DNA或者RNA中进行读码的时候会被看成G，从而实现A/T到G/C的突变。由于细胞对肌苷的切出修复不敏感，因而这种突变可以维持较高的产物纯度。

CBE系统是Cytidine base editor的简称，即嘧啶碱基转换技术，目前有BE1、BE2和BE3个工具，其中BE3的效率最高，因而在基因治疗，动物模型制作以及功能基因筛选等领域被广泛应用。

真核细胞例如哺乳动物细胞，包括人类细胞(人类原代细胞或已建立的人类细胞系)。所述细胞可以是非人类哺乳动物细胞，例如来自非人类灵长类动物(例如猴子)、奶牛/公牛/家牛、绵羊、山羊、猪、马、狗、猫、啮齿动物(例如兔子、小、大鼠、仓鼠)等。所述细胞来自鱼(例如鲑鱼)、鸟(例如禽鸟，包括小鸡、鸭、鹅)、爬行动物、贝类(例如牡蛎、蛤、龙虾、虾)、昆虫、蠕虫、酵母等。所述细胞可以来自植物，例如单子叶植物或双子叶植物。所述植物可以是粮食作物，例如大麦、木薯、棉花、花生、玉米、小米、油棕果、土豆、豆类、油菜籽或低芥酸菜子、大米、黑麦、高粱、大豆、甘蔗、糖甜菜、向日葵和小麦。所述植物可以是谷物(例如大麦、玉米、小米、大米、黑麦、高粱和小麦)。所述植物可以是块茎(例如木薯和土豆)。在一些实施方案中，所述植物可以是糖料作物(例如甜菜和甘蔗)。所述植物可以是含油作物(例如大豆、花生、油菜籽或低芥酸菜子、向日葵和油棕果)。所述植物可以是纤维作物(例如棉花)。所述植物可以是树木，例如桃树或油桃树、苹果树、梨树、杏树、核桃树、开心果树、柑橘属树(例如橙子、葡萄柚或柠檬树)、草、蔬菜、水果或藻类。所述植物可以是茄属植物；芸苔属(Brassica)植物；莴苣属(Lactuca)植物；菠菜属(Spinacia)植物；辣椒属(Capsicum)植物；棉花、烟草、芦笋、胡萝卜、卷心菜、西兰花、花椰菜、番茄、茄子、胡椒、生菜、菠菜、草莓、蓝莓、覆盆子、黑莓、葡萄、咖啡、可可等。

CRISPR(成簇规律间隔短回文重复序列)/Cas13(CRISPR相关蛋白13)介导的RNA编辑正在成为用于疾病诊疗、植物育种等方面的有前景的工具。

CRISPR是包含碱基序列的短重复的DNA基因座。每个重复之后是来自先前暴露于病毒的“间隔区DNA”的短区段。在约40％的测序的真细菌基因组和90％的测序的古细菌中发现CRISPR。CRISPR通常与编码与CRISPR相关的蛋白质的Cas基因相关。CRISPR/Cas系统是原核免疫系统，其赋予对外来遗传元件(例如质粒和噬菌体)的抗性并提供获得性免疫的形式。CRISPR间隔区识别并沉默真核生物体中的这些外源遗传元件(例如RNAi)。

CRISPR重复序列的大小为24至48个碱基对。它们通常显示一些二重对称，这意味着形成二级结构例如发夹，但不是真正的回文结构。重复序列被相似长度的间隔区分开。一些CRISPR间隔区序列与来自质粒和噬菌体的序列准确地匹配，尽管一些间隔区与原核生物的基因组匹配。响应于噬菌体感染，可迅速添加新的间隔区。

指导RNA(gRNA)。作为RNA指导的蛋白，Cas13需要短RNA以指导RNA靶标的识别。

Cas核酸酶。CRISPR相关(Cas)基因通常与CRISPR重复-间隔区阵列相关。截至2013年，已描述了超过四十个不同的Cas蛋白家族。在这些蛋白家族之中，Cas1看来在不同的CRISPR/Cas系统中是普遍存在的。Cas基因和重复序列结构的特定组合已用于限定8种CRISPR亚型(Ecoli、Ypest、Nmeni、Dvulg、Tneap、Hmari、Apern和Mtube)，其中一些与编码重复序列相关神秘蛋白(repeat-associated mysterious protein，RAMP)的另外的基因模块相关。在单个基因组中可存在多于一种CRISPR亚型。CRISPR/Cas亚型的散发性分布(sporadic distribution)表明该系统在微生物进化期间经历水平基因转移。

外源DNA明显地由Cas基因编码的蛋白质加工成小元件(长度为约30个碱基对)，然后以某种方式将其插入到靠近前导序列的CRISPR基因座中。来自CRISPR基因座的RNA是组成型表达的，并且被Cas蛋白加工成由具有侧翼重复序列的单独外源来源序列元件构成的小RNA。RNA指导其他Cas蛋白在RNA或DNA水平上沉默外源遗传元件。证据表明CRISPR亚型之间的功能多样性。Cse(Cas亚型Ecoli)蛋白(在大肠杆菌(E.coli)中称为CasA-E)形成功能性复合体Cascade，其将CRISPR RNA转录物加工成保留Cascade的间隔区-重复序列单元。在另一些原核生物中，Cas6加工CRISPR转录物。有趣的是，大肠杆菌中基于CRISPR的噬菌体灭活需要Cascade和Cas3，但不需要Cas1和Cas2。在激烈火球菌(Pyrococcus furiosus)和另一些原核生物中发现的Cmr(Cas RAMP模块)蛋白与小的CRISPR RNA形成功能性复合体，其识别和切割互补靶RNA。RNA指导的CRISPR酶被分类为V型限制酶。

除了利用已知Cas13家族其他成员寻找其他直系同源Cas蛋白外，我们还进行了从头寻找CRISPR-Cas13其他家族成员，技术路线如图1所示。

简单来说，该分析系统包括2大块，一部分CRISPR array区域的鉴定，我们首先下载NCBI和IMG截止到2021年2月份的全部细菌，古细菌基因组以及宏基因组的序列，利用CRISPR array鉴定软件(如Pilercr)进行鉴定CRISPR array区域；另一部分是该区域上下游附近Cas相关蛋白的搜寻，即取该区域上下游临近的6个蛋白，共计12个蛋白进行目标结构域分析和氨基酸(amino acid,AA)长度分析(300到950AA)。唯有同时满足二者，且氨基酸个数更小的单个Cas效应蛋白才是候选新型小分子量的Cas潜在蛋白。最终候选蛋白的氨基酸序列、早期HEPN结构域、拓展的HEPN结构域和坐标等信息参见表1。

其中本筛选体系的HEPN结构域除了包含过去Cas13家族成员中发现的RxxxxH(R4xH)特征(我们记为早期HEPN结构域)外，还进行了拓展，包括其他具有RNA切割活性的结构域RxxxxxH(R5xH)和RxxxxxxH(R6xH)(我们将RxxxxxH(R5xH)和RxxxxxxH(R6xH)以及RxxxxH(R4xH)总体记为拓展的HEPN结构域)。其中临近R保守氨基酸优选为N、Q、H或D，例如R[NDQH]xxxH、R[NDQH]xxxxH、R[NDQH]xxxxxH等组合；其中R4xH优选R[NDQH]xxxH，而R5xH和R6xH则优选R[NDQH]xxxxH和R[NQDH]xxxxxH；其中x代表任意氨基酸，而中括号内N、D、Q和H为优先考虑保守氨基酸。

候选蛋白筛到后将进行功能验证，我们采用图2所示实验技术路线图来进行操作。简而言之，我们首先将候选蛋白的核酸序列，DR序列以及target spacer序列送公司进行合成，然后将其导入表达质粒中构建相应的质粒。然后通过质粒转化在DH5a大肠杆菌感受态细胞进行质粒扩增培养，然后抽提质粒后进行人源293T(能表达红光)细胞系的转染试验，与此同时我们还设计了对应的阴性和阳性对照组来进一步确认候选蛋白的切割活性。共转染质粒48h后进行流式细胞分析等试验来最终确定候选蛋白的RNA酶切活性。

按照上述研究策略，我们随机选择了部分蛋白进行了验证。

如图3至11中所示，从DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102以及Cas13d的结果表明，我们候选的具有Rnase活性的Cas13蛋白在瞬转哺乳动物细胞系能够很好的敲低mCherry(红光)蛋白，而阴性对照组(图12)则不能切割，表现为红色荧光高亮；表明我们的候选蛋白可以在哺乳动物等上发挥Rnase的作用。而DZ87、DZ89、DZ95则显示比较弱的Rnase活性(如图11B和图13)，此外，从DZ75、DZ76、DZ103和DZ104的结果表明，这些Cas13蛋白在瞬转哺乳动物细胞系后不能有效敲低mCherry(红光)蛋白，在哺乳动物等上无法发挥Rnase的作用但是它们都有发挥Rnase活性的HEPN结构域，造成这种结果可能与该类候选蛋白具有很强的PFS偏好性，与我们随机选择的靶向mCherry的位置有关。类似地，为了验证候选蛋白广泛的适用于切割内源基因的多种RNA，我们开展了验证它们切割内源基因的能力。图14中则示出了利用DZ78候选蛋白能有效切割内源基因，如Ptbp1，Rest，EGFR，NF2等基因。表明其具有靶向不同RNA的广泛的切割效果。

为了进一步研究候选蛋白DZ78的发挥Rnase活性的特性，我们从其靶向的偏好性(PFS)，向导RNA成熟机制以及靶向RNA的spacer长度等方向展开探索(如图18所示)：

为了探索DZ78的靶向RNA的位置偏好性，我们通过在mcherry末尾设计一段供sgRNA靶向切割且这个target位置前后各有4个连续相同的碱基的mCherry表达质粒，然后通过共转染靶向不同组合的mCherry表达质粒48h后进行流式分析，可以发现(如图18A所示)DZ78在靶向mcherry的target位置时并没有展示很强的碱基序列偏好性，提示DZ78蛋白在靶向RNA时的位置可能更加广泛，具有更加广泛的应用价值。

为了探索候选蛋白DZ78的guide RNA的成熟机制，通过设计不同4种sgRNA：包括DR-sg-DR(即为double DR)，DR-sg(即为5’方向DR)，sg-DR(即为3’方向DR)以及非靶向目标RNA的control sgRNA。同时为了验证其在细胞核内和细胞核外的Rnase活性，还对sgRNA进行添加入核信号(NLS)。从结果可以发现(如图18B所示)，不论是细胞核内还是细胞核外，候选蛋白DZ78只有在于双DR或者3’方向DR共转染时才能发挥其Rnase活性，表明候选蛋白DZ78的guide RNA成熟体系为3’方向。

为了探索候选蛋白靶向RNA的spacer长度对其发挥Rnase活性效率的影响，通过设计不同spacer长度的guide RNA共转染质粒(如图18C所示)，可以看到不同spacer长度对giude RNA引导候选蛋白DZ78靶向切割mCherry的影响很大(如图18D所示)，其中当spacer长度低于25bp的时候效率降低很多，而30bp～50bp间差异不是很大。表明候选蛋白DZ78在靶向RNA的时候其spacer长度会影响其切割RNA的效率。

当前用于单碱基编辑的系统主要有两种，一种是ABE系统，另一种是CBE系统。简单来说，通过候选Cas13蛋白的切割结构域(拓展的HEPN结构域)进行突变处理，获得只有结合RNA而没有切割活性的候选dCas13蛋白，然后融合adar酶序列，构建ABE单碱基编辑系统的质粒，然后对特定序列，比如TP53基因的转录本进行定点碱基突变处理的sgRNA设计并构建相应的质粒载体。然后通过共转染人源293T细胞系，48小时后进行流式细胞分选获得共转染的细胞系。然后进行RNA转录本的提取以及建库。然后进deep seq测序。测序结束后通过生物信息方法分析TP53基因转录本的突变情况就可以获得对应的ABE系统的单碱基编辑效能分析。从而通过不断的优化sgRNA来实现构建目标区域的最优单碱基编辑系统。

依据未知蛋白在已知蛋白的覆盖度越高且相似度占比越大则未知蛋白与已知蛋白的同源性越近的原理进行。对所筛选到的候选蛋白后，我们先从NCBI数据库以及专利文献中下载Cas13a,Cas13b,Cas13c，Cas13d，Cas13X，Cas13Y等的相关蛋白序列，然后与我们的数据一起合并构建本地blastp的索引文件，然后将候选蛋白序列比对到本地blastp索引库中进行蛋白序列比对分析。对于蛋白之间相似度(identity)小于20％或者没法比对到本地索引库的部分我们统一标注为20％；类似的，对于覆盖度(coverage)小于5％或者没法比对到本地索引库的标记为1％。从众多候选Cas13蛋白中选取与已知Cas13蛋白同源性低的新Cas13蛋白。随机筛选靶向mCherry蛋白kncok down实验具有Rnase活性的候选Cas13蛋白DZ41、DZ77、DZ78、DZ79、DZ81、DZ82和DZ102的同源性分析结果参见表5。从表5可以看出，本发明方法所鉴定出的新Cas13蛋白与已知各家族Cas13蛋白的同源性水平极低。

候选蛋白信息参见下表1。

筛选所用的RNA酶和相应保守型结构域的序列信息汇总参见下表2和表3。

具有Rnase活性的候选Cas13蛋白的DR序列信息参见下表4。

具有Rnase活性的候选Cas13蛋白的同源性分析结果参见下表5。

候选Cas蛋白验证实验所用引物和spacer序列参见下表6。

序列表

<110> 中国科学院脑科学与智能技术卓越创新中心

<120> 新型Cas13蛋白及其筛选方法和应用

<150> 202110625339.0

<151> 2021-06-04

<160> 303

<170> SIPOSequenceListing 1.0

<210> 1

<211> 524

<212> PRT

<213> 宏基因组(metagenomic)

<400> 1

Met Lys Val Ser Lys Val Lys Val Ser Val Gly Asn Asn Glu Lys Gln

1 5 10 15

Met Met Thr Met Phe Arg Asn Ser Asn Lys Gly Ala Leu Val Tyr Trp

20 25 30

Asp Asp Lys Ser Arg Asp Asp Gln Thr Glu Arg Ile Ile Pro Gly Gln

35 40 45

Lys Met Glu Asn Phe Ala Leu Ser Ile Leu Asn Gln Thr Leu Val Lys

50 55 60

Lys Gly Val Phe Leu Ser Met Leu Arg Met Gly Thr Ser Gly Lys Val

65 70 75 80

Ala Ser Lys His Ala Asn Gly Thr Glu Met Arg Val Thr His Lys Glu

85 90 95

Lys Glu Lys Ala Gly Lys Ala Tyr Glu Ser Ile Arg Ala Leu Leu Ala

100 105 110

Phe Val Leu Ser Ser Asp Phe Gly Ser Arg Glu Phe Lys Lys Asn Val

115 120 125

Pro Lys Glu Ile Glu Arg Ser Leu Leu Asp Cys Met Ile Thr Lys Lys

130 135 140

Phe Arg Glu Glu Ile Tyr Leu Met Asp Glu Lys Thr Gly Glu Lys Arg

145 150 155 160

Arg Leu Thr Asp Leu Ile Leu Glu Ala Leu Ser Ser Gly Asp Val Leu

165 170 175

Ile Leu Thr Pro Tyr Val Lys Trp Arg Asp Asp Phe Val Ala Leu Lys

180 185 190

Ser Ser Phe Leu Arg Arg Ser Ile His Asn Asn Arg Ile Thr Val Ala

195 200 205

Asn Gly Gly Ser Lys Arg Met Ser Val Leu Glu Ala Trp Ser Glu Ala

210 215 220

Leu Ile Ser Pro Glu Lys Asp Gln Thr Glu Lys Asn Lys Val Gln Gly

225 230 235 240

Phe Ser Lys Ile Asn Ala Ile Ser Glu Val Pro Thr Arg Tyr Asn Ile

245 250 255

Asp Leu Leu Ile Lys Asn Leu Asn Lys Val Glu Met Gly Glu Phe Lys

260 265 270

Asp Asn Gly Thr Leu Lys Arg Gly His Glu Phe His Lys Arg Leu Lys

275 280 285

Val Cys Leu Gln Thr His Gln Lys Thr Ile Phe Gly Thr Arg Asp Asn

290 295 300

Pro Asn Leu Thr Asn Arg Gly Asp Asn Glu Leu Tyr Cys Tyr Asn Leu

305 310 315 320

Glu Val Val Lys Tyr Leu Asn His Phe Phe Pro Ile Asn Val Pro Ser

325 330 335

Ala Lys Arg Leu Thr Lys Asp Arg Ile Leu Tyr Tyr Leu Asn Glu Lys

340 345 350

Thr Met Lys Arg Thr Ile Glu Ala Gln Leu His Asn Ala Leu Arg Ala

355 360 365

Asn Leu Ile Arg Asn Gly Lys Leu Arg Trp His Asp Leu Leu Gly Arg

370 375 380

Asp Asp Ile Thr Asn Lys Asp Leu Ile Thr Leu Lys Met Asp Glu Gly

385 390 395 400

Phe Leu Leu Ser Ile Ile Asp Ala Cys Ala Phe Ala Gly Asn Asn Val

405 410 415

Arg Asn Ile Ile Asp Arg Tyr Gln Thr Gly Asp Ile Ile Tyr Lys Asp

420 425 430

Ile Leu Lys Lys Ser Ile Glu Lys Gly Val Ser Asp Gly Pro Leu Phe

435 440 445

Gly Leu Phe Phe Asn Ile Glu Asp Ser Gln Pro Ile Leu Thr Lys Asp

450 455 460

Leu Trp Ala Leu Arg Gly Ala Val Gln Lys Ile Arg Asn Asp Ile Phe

465 470 475 480

His Tyr Met Phe Asn Leu Pro Asn Asn Asp Gly Gly Met His Asp Asp

485 490 495

Arg Ser Ala Thr Lys Val Lys Thr Ile Leu Asn Val Thr Glu Phe Glu

500 505 510

Tyr Asp Gly Asp Asn Lys Thr Asp Lys Ser Ser Arg

515 520

<210> 2

<211> 987

<212> PRT

<213> 宏基因组(metagenomic)

<400> 2

Met Glu Arg Ser Gly Lys Arg Val Ser Leu Glu Val Ala Gly Arg Val

1 5 10 15

Leu Phe Glu His Trp Pro Lys Val Phe Phe Asp Pro Leu Ser Arg Lys

20 25 30

Ala Leu Gly Ile Ala Asp Ala Arg Asn Ser His Pro Gly Leu Phe Ser

35 40 45

Leu His Met Ala Val Lys Asp Ala Tyr Ala Arg Val Leu Lys Arg His

50 55 60

Lys Lys Gly Ala Gly Ala Gly Ala Arg Lys Gly Ser Gln Ala Thr Ala

65 70 75 80

Arg Leu Ser Val Ile Leu Pro Lys Asp Met Pro Gly Leu Ile Arg Leu

85 90 95

Ile Thr Ala Gln Lys Asp Asn Gln Asn Ile Asn Arg Gln Ile Arg Leu

100 105 110

Gly Arg Ile Leu His Tyr Thr Ala Ser Gly Glu Trp Ser Asp Ser Thr

115 120 125

Thr Ala Val Asp Ala Lys Trp Pro Thr Asp Ile Leu Glu Ser Pro Phe

130 135 140

Trp Ala Ser Asp Gly Gln Ala Lys Ile Lys Arg Ala Glu Ala Phe Val

145 150 155 160

Arg Val Trp Arg His Gln Ile Ala Leu Ala Arg Leu Thr Leu Thr Asp

165 170 175

Trp Ala Ser Met Arg Lys Pro Leu Ser Arg Asp Ile Leu Gly Asp Arg

180 185 190

Ala Ala Ala Asn Leu Val Ile His Gln Asp Asn Phe Ser Ser Glu Leu

195 200 205

Phe Asp Arg Lys Ala Ala Leu Leu Phe Gly Val Gln Ser Lys Ile Phe

210 215 220

Ala Ala Asp Asp Ala Ser Lys Lys Glu Val Leu Lys Cys Val Ile Glu

225 230 235 240

Glu Met Ser Glu Leu Arg Asn Gln Ala Phe His Phe Lys Gly Leu Arg

245 250 255

Glu Phe Leu Val Ser Ile Asp Arg Leu Ser Phe Ser Asp Leu Val Gln

260 265 270

Lys Ser Ala Arg Gln Ile Trp Glu Ala Asp Ser Ser Arg Arg Ser His

275 280 285

Arg Leu Lys Glu Thr Leu Arg Ala Ala His Ala Glu Val Tyr Phe Ser

290 295 300

Ala Thr Gln Cys Glu Ala Leu Leu Arg Arg Val Thr Thr Gln Val Asp

305 310 315 320

Ser Asp Leu Pro Leu Pro Arg Phe Ser Arg Leu Leu Arg Arg Ala Lys

325 330 335

Ser Cys Pro Asp Arg Ala Ala Ile Lys Leu Pro Pro Pro Ala Asn Arg

340 345 350

Ser Asp Leu Glu Gln Pro Trp Arg Leu Cys Gln Tyr Thr Ala Leu Lys

355 360 365

Leu Leu Tyr Glu Arg Pro Phe Arg Thr Trp Leu Glu Ala Arg Ser Ala

370 375 380

Asp Glu Leu Asn Ala Trp Ile Gly Arg Ala Val Gln Arg Ala Thr Asp

385 390 395 400

Ala Ala His Ser Gln Asn Tyr Arg Lys Tyr Lys Leu Ala Gln Lys Val

405 410 415

Ile Ser Ala Arg Ala Ser Ser Leu Pro Arg Pro Thr Lys Gly Gln Lys

420 425 430

Ile Arg Asp Phe Phe Phe Ala Leu Ser Ser Ala Thr Ala Ser Glu Met

435 440 445

Arg Val Gln Arg Gly Tyr Glu Ser Asp Gly Glu Lys Ala Arg Asn Gln

450 455 460

Ala Asp Phe Ile Asp Asn Leu Leu Cys Asp Val Met Ser Leu Ala Leu

465 470 475 480

Cys Gln Phe Ile Ser Ser Glu Ala Phe Leu Trp Ile Leu Ile Ala Pro

485 490 495

Val Asp Pro Tyr Leu Val Gly Lys Arg Lys Cys Gln Leu Asp Ala Phe

500 505 510

Glu Leu Pro Ile Pro Ser Phe Glu Ala Lys Glu Trp Gln Val Ser Leu

515 520 525

Tyr Phe Leu Leu His Leu Ile Pro Val Gly Asp Val Ala Gln Leu His

530 535 540

His Gln Val Ala Lys Trp Glu Ile Thr Ala Gly Arg Asp Glu Gly Ile

545 550 555 560

Glu Leu Glu Asp Met Asn Arg Ile Leu Arg Leu Gln Thr Thr Leu Lys

565 570 575

Leu Tyr Met Asp Met His Asp Glu Lys Phe Glu Gly Asp Ala Arg Leu

580 585 590

Glu Gly Cys Glu Gly Phe Arg Asp Leu Phe Glu Thr Asn Ser Gly Phe

595 600 605

Glu Gln Val Phe Pro Lys Ser Ser Ser Pro Asp Asp Asp Arg His Leu

610 615 620

Pro Arg Arg Gly Leu Arg Glu Ile Met Arg Tyr Gly His Leu Gln Met

625 630 635 640

Val Met Gly Phe Leu Pro Lys Gln Lys Ile Ser Asp Gly Glu Val Ala

645 650 655

Glu Tyr Leu Glu Thr Met Arg Ala Ser Gly Pro Asn Gly Gln Ser Glu

660 665 670

Ile Ala Ile His Gln Ala Gln Arg Glu Glu Leu His Glu Lys Trp Ser

675 680 685

Arg Ala Arg Pro Gln Leu Leu Ser Gly Asp Glu Tyr Arg Ala Tyr Cys

690 695 700

Glu Val Leu Thr Lys Ile Val Lys His Arg Gln Ala Ala Ala Arg Ile

705 710 715 720

Asn Leu Thr Ala Ile Val His Phe His His Gln Val Met Thr Leu Leu

725 730 735

Gly Arg Leu Ala Asp Phe Ser Gly Leu Phe Glu Arg Asp Leu Tyr Phe

740 745 750

Val Thr Leu Ala Ile Leu Tyr Gln Tyr Gly Leu Ser Pro Gln Arg Ala

755 760 765

Phe Glu Asp Lys Gly Leu Gly Tyr Leu Lys Glu Gly Arg Ile Phe Lys

770 775 780

Ala Leu Glu Asn Leu Ile Ser Glu His Lys Gly Lys Ile Lys Val Glu

785 790 795 800

Leu Lys His Tyr Phe Gly Pro Glu Trp Asp Ser Trp Asp Gly Arg Arg

805 810 815

Gly Thr Arg Asn Arg Leu Ala His Phe Asn Met Leu Arg Ser His Ala

820 825 830

Pro Arg Leu Asp Met Thr Glu Trp Val Asn Ser Thr Arg Arg Leu Met

835 840 845

Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Gln Ser Ile Arg Glu

850 855 860

Met Met Lys Arg Asn Gly Ile Glu Leu Ser Trp Gln Met Asp Thr Gly

865 870 875 880

Gly Lys Asn His Glu Leu Thr Ser Ala Val Val Ser Ser Ala Ser Val

885 890 895

Val His Leu Gly Gly Ile Arg Leu Val Glu Phe Ser Gly Ser Ala Ser

900 905 910

Asn Ser Asn Lys Thr Gln Ala Ile Thr Glu Ala Leu His Gly Thr Pro

915 920 925

Tyr Val Gln Met Val Ala Ala Leu Phe Gly Gly Lys Thr Gln Gln Phe

930 935 940

Asp Asp Ile Thr Gly Arg Asp Leu Ser Asn Ile Asp Trp Gln Ser Thr

945 950 955 960

Glu Arg Lys Ala Gln Asn Thr Gly Gly Arg Gly Ala Met Gly Thr Leu

965 970 975

Arg Pro Arg Arg Glu Glu Lys Val Arg Arg Glu

980 985

<210> 3

<211> 844

<212> PRT

<213> 宏基因组(metagenomic)

<400> 3

Met Ala Thr Ala Val Ser Ile Gly Lys Leu Ile His Tyr Gln Gly Gly

1 5 10 15

Ile Glu Ala Ile Gly Asn Lys Glu Asp Leu Val Asn Ser Lys Phe Leu

20 25 30

Thr Asp Ala Gly Leu Thr Glu Ile Lys Gln Asn Glu Ser Phe Val Arg

35 40 45

Gln Trp Leu Glu Leu Ile Ala Ile Ala Asn Thr Thr Leu Ser Gln Leu

50 55 60

Val Asp Pro Asp Gly Lys His Glu Asp Ile Phe Ala Ala Thr Ser Phe

65 70 75 80

Asp Asp Ala Leu Lys Gly Leu Asn Asn Ile Ser Asp Phe Asp Ser Lys

85 90 95

Phe Lys Leu Leu Phe Gly Glu Asn His Arg Leu Tyr Asp Asp Asp Thr

100 105 110

Glu Arg Gln Lys Leu Leu Arg Ile Ile Tyr Asp Thr Ile Ser Ala Leu

115 120 125

Arg Asn Ala Ser Phe His Phe Lys Asn Ile Gln Gly Phe Asn Lys Ala

130 135 140

Leu Glu Asp Asn Leu Ser Thr Lys Gly Arg Arg Gln Lys Gly Val Val

145 150 155 160

Asp Lys Ile Ile Glu Tyr Thr Lys Val His Gln Gln Lys Gln His Glu

165 170 175

Leu Leu Ile Ala Asp Leu Lys Ala Ala Asn Val Glu Asp Tyr Leu Ser

180 185 190

Gln Leu Gln Leu Asp Tyr Leu Phe Glu Val Val Cys Lys Gly Glu Arg

195 200 205

Glu Leu Leu Asp Met Pro Lys Phe Ser Lys Leu Leu Leu Arg Ala Gly

210 215 220

Glu Ile Gly Glu Lys Ile Ile Ser Pro Val Asn Ala Thr Ala Met Glu

225 230 235 240

His Pro Ala Tyr Gln Cys Cys Phe Ile Ser Leu Lys Met Leu Tyr Asp

245 250 255

Gln Asp Phe Val Asn Trp Leu Lys Lys Gln Ser Asn Lys Asn Ile Ala

260 265 270

Ser Trp Met Asp Ser Ala Lys Thr Arg Ala Thr Asn Ala Ala Lys Lys

275 280 285

Ile Phe Arg Asn Gly Thr Asn Ile Ser Ser Lys Met Ala Arg Leu Arg

290 295 300

Asn Ile Val Glu Asp Glu Thr Leu Val Glu Tyr Phe Ser Phe Ile Thr

305 310 315 320

Ala Glu Thr Ala Asn Glu Phe Gln Val Gln Gln Gln Lys Asn Arg Tyr

325 330 335

Gln Ser Asn Ser Ala Ser Ala Lys Glu Gln Ser Asn Phe Val Glu Gln

340 345 350

Phe Lys Gln Asp Phe Leu Ile Tyr Ala Phe Lys Gly Tyr Ile Gly Asp

355 360 365

Ile Lys Phe Gly Lys Leu Asp Gln Gly Glu Lys Leu Leu Lys Cys Asn

370 375 380

Ala Lys Gly Ser Leu Leu Pro Glu Asn Asn Arg Ser Asp Thr Gly Ala

385 390 395 400

Glu Asp Ile Glu Arg Pro Trp Leu Tyr Leu Val Leu His Leu Val Pro

405 410 415

Ile Glu Val Val Asn Arg Ile Thr Leu Gln Ile Lys Lys His Ser Val

420 425 430

Leu Thr Asn Ser Thr Leu Asp Asp Thr Arg Ala Ala Tyr Thr Asp Ile

435 440 445

His Gln Ala Phe Ser Leu Tyr Leu Gly Val His Gly Ala Ile Leu Gly

450 455 460

Leu Gln Ser Leu Ser Asn Glu Pro Glu Leu Gln Val Phe Phe Glu Lys

465 470 475 480

Glu Ser Asp Phe Asn Asn Leu Phe Thr Asp Asn Gly Glu Gly Leu Val

485 490 495

Pro Val Lys Gly Leu Arg Asp Ile Leu Arg Phe Gly Asn Leu Glu Gln

500 505 510

Leu Lys Lys Met Phe Ser Asp Lys Lys Val Ala Ser Asp Asp Ile Thr

515 520 525

Asn Leu Lys Glu Tyr Leu Glu Ala Thr Gly Gly Lys Ser Lys Ile Ala

530 535 540

Arg Ala Gln Glu Lys Arg Ile Glu Leu His Lys Thr Leu Thr Glu Leu

545 550 555 560

Pro Lys Arg Leu Thr Lys Pro Arg Val Lys Gln Thr Gly Ile Asp Thr

565 570 575

Tyr Glu Lys Asn Asn Asn Ile Ser Ile Ser Gly Ser Ile Ser Glu Tyr

580 585 590

Arg Lys Asp Leu Lys Cys Ile Val Ser Tyr Arg Glu Leu Lys Asn Lys

595 600 605

Ile Tyr Leu Tyr Asn Val Lys Gln Ala His Gln Leu Ile Met Ala Thr

610 615 620

Gln Ala Arg Leu Leu Gly Tyr Ser Gln Ile Arg Glu Arg Asp Leu Tyr

625 630 635 640

Phe Val Leu Leu Thr Gln Leu Met Leu Arg Gly Val Thr Leu Glu Lys

645 650 655

Glu Lys Lys Lys Lys Asp Lys Asp Lys Leu Asn Ala Leu Asp Thr Asp

660 665 670

Thr Val Leu Tyr Glu Glu Glu Lys Lys Lys Leu Glu Lys Gln Ile Lys

675 680 685

Pro Glu Arg Ser Leu Lys Asp Leu Val Glu Lys Gly Leu Ile Phe Leu

690 695 700

Ala Leu Asp Gln Leu Ser Ser Ser Asp Lys Asp Leu Lys Ala Ile His

705 710 715 720

Ser Glu Ile Glu Asp Met Phe Val Gly Val Ser Pro Asp Ser Asp Asn

725 730 735

Arg Asn Leu Arg Asn Arg Leu Ala His Phe Lys Asp Leu Gly Asn Lys

740 745 750

Asn Leu Ile Asn Ile Thr Ser Gln Ile Asn Glu Val Arg Lys Met Met

755 760 765

Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys Ser Met Ile Asp

770 775 780

Leu Phe Glu Arg Tyr Asn Leu Ile Leu Ser Phe Lys Val Gln Ser His

785 790 795 800

Lys Leu Gln Leu Lys Asn Leu Lys Ser Lys Gln Ile Thr His Leu Asn

805 810 815

Asn Lys Gly Ile Thr Glu Asn Leu Leu Ser Asp Asp Tyr Val Ser Val

820 825 830

Ile Lys Arg Leu Leu Leu Thr Glu Gln Asn Pro Glu

835 840

<210> 4

<211> 512

<212> PRT

<213> 宏基因组(metagenomic)

<400> 4

Met Ala Asn Arg Leu Ile Leu Ala Val Leu Val Leu Val Val Leu Gly

1 5 10 15

Ala Leu Tyr Ala Ala Ala Gly Leu Asn Arg Pro Ile Thr Leu Thr Ser

20 25 30

Ala Ala Ala Ala Gly Ala Pro Gly Gln Leu Pro Val Thr Ser Ala Leu

35 40 45

Leu Ala Cys Pro Gly Pro Gly Ser Asp Gly Pro Thr Gly Gly Gly Leu

50 55 60

Ala Ile Ala Ser Ala Pro Ala Thr Ala Ser Thr Gly Gln Val Leu Leu

65 70 75 80

Thr Gly Leu Asn Pro Ala Gly Gly Thr Gly Ser Gly Asp Gly Ala Gly

85 90 95

Thr Thr Leu His Ile Leu Thr Gln Pro Gly Val Leu Thr Leu Ala Arg

100 105 110

Val Pro Lys Ala Pro Pro Val Pro Lys Asn Leu Val Ala Ala Gln Asn

115 120 125

Met Ala Gly Gly Leu Val Pro Thr Ser Thr Ile Arg Gly Gly Val Val

130 135 140

Ile Gln Ala Val Gly Ala Met Ala Gln Gly Leu Asp Ala Glu Gln Leu

145 150 155 160

Asn Pro Ala Gly Gln Pro Thr Ala Arg Cys Gly Ala Pro Gly Ser Asp

165 170 175

Phe Trp Phe Val Ser Pro Val Thr Leu Thr Leu His Ile Glu Leu Tyr

180 185 190

Leu Leu Asn Ala Asp Asn Val Pro Ala Ser Ala Asp Val Ser Val Gln

195 200 205

Thr Asp Ser Gly Pro Leu Leu Gly Ser Pro Asp Ser Gly Ile Val Val

210 215 220

Pro Pro His Ser Leu Val Ala Gln Ser Leu Asp Lys Leu Val His Ala

225 230 235 240

Ala Arg Ala Val Ser Leu His Val Thr Thr Ser Val Gly Arg Val Val

245 250 255

Ala Ala Val Arg Glu Thr Asn Ser Lys Thr Gln Pro Gly Ser Trp Leu

260 265 270

Pro Ala Ala Gln Pro Ala Ala Thr His Gln Val Leu Ala Gly Leu Pro

275 280 285

Gly Val Pro Gly Thr Pro Glu Leu Tyr Val Ala Val Pro Gly Asn Ala

290 295 300

Ala Ala Leu Val Lys Val Thr Ala Val Thr Pro Arg Gly Ser Tyr Gln

305 310 315 320

Pro Thr Gly Gly Asn Gly Ile Ser Leu Leu Gly His Leu Thr Thr Gly

325 330 335

Ile Pro Ile Pro Ser Leu Gly Gly Ile Pro Ala Ser Ile Ser Val Ser

340 345 350

Ala Asn Val Pro Val Thr Ala Val Leu Glu Ile Pro Gly Gly Pro Ala

355 360 365

Gly Ala Leu Gly Ala Val Ile Ser Gly Ser Asp Pro Ile Gln Glu Gln

370 375 380

Gly Val Val Ala Ala Ser Pro Val Gly His Gly Val Ala Thr Glu Leu

385 390 395 400

Val Leu Ser Ala Pro Gly Gln Ala Ala Ser Val Arg Ile Ala Gln Ala

405 410 415

Leu Pro Gly Ala Val Leu Thr Gly Gln Pro Gly Gln Val Val Gln Val

420 425 430

Pro Ala Arg Ser Thr Val Val Val His Val Thr Leu Pro Arg Arg Ser

435 440 445

Ala Ser Ala Ser Gln Val Ala Leu Val Val Thr Pro Leu Pro Lys Ser

450 455 460

Gly Pro Val Tyr Ala Ala Arg Ala Ala Phe Ser Gly Gly Thr Val Gln

465 470 475 480

Thr Ile Leu Pro Val Thr Thr Ala Pro Thr Gln Ile Ser Leu Pro Asp

485 490 495

Ala Arg Glu Ser Leu Ile Thr Ile Leu Gly Leu Ser Ser Ala Asp Arg

500 505 510

<210> 5

<211> 400

<212> PRT

<213> 宏基因组(metagenomic)

<400> 5

Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Asp Arg Pro Val Ser Ala

1 5 10 15

Gly Thr Gln Glu Ile Ile Ala Ser Met Glu Pro Arg Arg Ile Arg Arg

20 25 30

Gly Asn Ser Trp Ala Gly Leu Ser Asn Arg Lys Thr Cys Lys Arg Leu

35 40 45

Gln Trp Ser Arg Gly Glu Phe Ala Ala Glu Thr Cys Arg Ser Ala Arg

50 55 60

Gly Arg Thr Pro Pro Pro Ala Cys Phe Asn Gly Ala Ala Ala Asn Ser

65 70 75 80

Pro Arg Lys Arg Glu Gly Cys Arg Gly Gly Gln Gly His Arg Pro Ala

85 90 95

Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Ala Glu Leu Leu

100 105 110

Pro Pro Lys Gly Glu Leu Leu Gly Leu Gln Trp Ser Arg Gly Glu Phe

115 120 125

Ala Ala Glu Thr Cys Arg Arg Gly Gly Leu Trp Leu Ser Ala Gly Ala

130 135 140

Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Ser Leu Cys

145 150 155 160

Ser Trp Leu Arg Leu Arg Ser Ala Gly Phe Asn Gly Ala Ala Ala Asn

165 170 175

Ser Pro Arg Lys Arg Glu Cys Ala His Pro Glu His Thr Gly Met Trp

180 185 190

Val Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Gly Glu Arg

195 200 205

Trp Pro Val Leu Asp Asp Asp Asp Glu Ala Ser Met Glu Pro Arg Arg

210 215 220

Ile Arg Arg Gly Asn Arg Ala Val Arg Gly Arg Lys Arg Glu Ala Ala

225 230 235 240

Pro Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Arg Ala

245 250 255

Gln Pro Asn Pro Glu Lys Arg His Glu Arg Phe Asn Gly Ala Ala Ala

260 265 270

Asn Ser Pro Arg Lys Arg Ser Ala Arg Gly Leu Arg Ala Asp His Pro

275 280 285

Val Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Arg Lys Asp

290 295 300

Asp Asp Cys Val Arg Ser Asp Pro Asp Ser Phe Asn Gly Ala Ala Ala

305 310 315 320

Asn Ser Pro Arg Lys Pro Arg Ile Ala Arg Gln Arg Gln Leu Met Glu

325 330 335

Ser Arg Phe Asn Gly Ala Ala Ala Asn Ser Pro Arg Lys Arg Pro Gly

340 345 350

Ala Ala Leu Pro Arg Leu Gln Arg Arg Arg Phe Asn Gly Ala Ala Ala

355 360 365

Asn Ser Pro Arg Lys Pro Arg Pro Gly Ala Arg His Asp Arg Asp Ser

370 375 380

Gly Ala Ser Met Glu Pro Arg Arg Ile Arg Arg Gly Asn Ala Arg Ser

385 390 395 400

<210> 6

<211> 562

<212> PRT

<213> 宏基因组(metagenomic)

<400> 6

Met Glu Thr Ala Gln Lys Val Asn Asn Val Gly Lys Arg Ile Arg Tyr

1 5 10 15

Asn Pro Phe Lys Thr Val Asp Lys His Tyr Phe Gly Gly Phe Phe Asn

20 25 30

Leu Ala Glu Asn Asn Ile Gln Glu Val Phe Glu Glu Ile Asn Leu Arg

35 40 45

Leu Lys Asn Lys Asn Ala Pro Ile Lys Asn Ile Ile Glu Lys Tyr Thr

50 55 60

Asp Glu Asn Ile Ser Leu Val Glu Tyr Glu Lys Phe Ile Ala Ile Leu

65 70 75 80

Ser Glu Tyr Phe Pro Ile Ala Lys Glu Ile Asp Gln Lys Asn Lys Lys

85 90 95

Thr Phe Asn Asp Lys Ile Ile Glu Lys Thr Arg Ile Glu Arg Val Ser

100 105 110

Asp Phe Arg Lys Asp Phe Phe Leu Phe Ile Lys Ala Ile Glu Lys Leu

115 120 125

Arg Ser Tyr Tyr Thr His Phe Lys His Asp Asp Ile Ile Ile Asp Asn

130 135 140

Gln Leu Phe Asn Leu Leu Asp Asn Ile Leu Leu Asn Thr Val Leu Glu

145 150 155 160

Thr Lys Lys Lys Tyr Leu Lys Thr Asp Lys Thr Lys Glu Leu Leu Lys

165 170 175

Asp Ser Leu Gln Glu Glu Leu Glu Lys Leu Tyr Glu Leu Lys Ile Asn

180 185 190

Gln Leu Glu Glu Lys Lys Arg Glu Val Asn Ala Leu Ile Ala Glu Gln

195 200 205

Lys Lys Asn Gly Glu Glu Thr Gln Arg Pro Phe Arg Tyr Ser Thr Asp

210 215 220

Lys Asp Gln Ile Ile Asn Ser Ile Tyr Asn Asp Ala Ile Lys Pro Phe

225 230 235 240

Leu Tyr Glu Asn Ala Asn Lys Val Glu Ile Ser Asn Lys Lys Lys Thr

245 250 255

Ala Phe Asn Glu Lys Asp Glu Leu Val Lys Glu Gly Asp Phe Asn Leu

260 265 270

Pro Ile Ser Ser Ser Gly Ile Val Phe Leu Leu Ser Cys Phe Leu Asn

275 280 285

Arg Lys Glu Ile Glu Asp Leu Lys Ala Asn Ile Lys Gly Tyr Lys Gly

290 295 300

Lys Val Ile Lys Gly Asp Val Leu Asp Leu Glu Lys Asn Ser Ile Arg

305 310 315 320

Phe Met Ala Thr His Arg Ile Tyr Ser Val Met Cys Tyr Lys Gly Leu

325 330 335

Lys Asn Lys Ile Arg Thr Ser Gln Ser Thr Thr Lys Glu Thr Leu Leu

340 345 350

Met Gln Met Ile Asp Glu Leu Ser Lys Ile Leu Asp Val Leu Tyr Lys

355 360 365

Asn Ile Ser Glu Asp Leu Gln Asn Thr Phe Thr Glu Asp Trp Asn Glu

370 375 380

Tyr Tyr Lys Asp Asn Phe Glu Asn Leu Asp Asn Leu Glu Asn Ser Lys

385 390 395 400

Val Ile His Pro Val Ile Arg Lys Arg Tyr Glu Asp Lys Phe Asn Tyr

405 410 415

Phe Ala Ile Arg Phe Leu Asp Glu Phe Val Asp Phe Pro Ser Leu Arg

420 425 430

Phe Gln Val His Leu Gly Asn Tyr Ile Lys His Ser Met Ser Lys Asn

435 440 445

Val Gly Asn Val Ile Thr Thr Arg Glu Ile Lys Asn Lys Ile Phe Val

450 455 460

Phe Gly Arg Leu Asp Glu Ile Asn Gln Tyr Lys Ser Asp Phe Phe Asn

465 470 475 480

Lys Ile Lys Glu Asn Glu Glu Glu Thr Asn Trp Glu Leu Phe Pro Asn

485 490 495

Pro Asn Tyr His Phe Pro Met Glu Asn Ser Asp Glu Leu Lys Tyr Ala

500 505 510

Asn Lys Ile Gly Ile Tyr Leu Asp Leu Trp Lys Ser Lys Glu Ile Ile

515 520 525

Asn Asp Glu Ile Glu Asn Lys Asn Lys Gln Thr Ser Ser Tyr Lys Lys

530 535 540

Asp Leu Val His Gln Ile Thr Lys Glu Lys Lys Arg Tyr Ile Leu Gly

545 550 555 560

Asn Gln

<210> 7

<211> 522

<212> PRT

<213> 宏基因组(metagenomic)

<400> 7

Met Thr His Glu Pro Thr Gln Lys Ala Leu Phe Gly Ala Phe Leu Asn

1 5 10 15

Thr Ala Gln His Asn Ala Tyr Leu Ile Ile Asn Glu Val Asn Glu Lys

20 25 30

Leu Gly Lys Ala Asp Val Glu Glu Gly Lys Leu Asp Asn Asp Ala Tyr

35 40 45

Ala Leu His Ile Leu Thr Asn Lys Glu Ile Lys Thr Leu Pro Leu Lys

50 55 60

Ile Leu Ser Arg Arg Leu Leu Met Glu Gly Phe Pro Phe Leu Cys Ala

65 70 75 80

Leu Gly Phe Ser Gln Asp Ala Thr Gln Thr Asp Asn Phe Glu Ala Leu

85 90 95

Lys Thr Lys Leu Gln Lys Ala Leu Lys Thr Leu Asn Glu Tyr Arg Asn

100 105 110

Phe Tyr Ser His Tyr Tyr Glu Gln Ser Ile Asp Trp Gln Lys Cys Gln

115 120 125

Phe Ala Asp Leu Asp Leu Ile Arg Glu Asp Phe Ile Lys Thr Phe Thr

130 135 140

Thr Tyr Ala Ser Glu Glu Gln Val Ser Lys Ser Tyr Glu Glu Val Lys

145 150 155 160

Lys Lys Lys Glu Glu Leu Asp Lys Cys Lys Lys Gly Leu Ser Leu Ala

165 170 175

Lys Arg Lys Lys Gln Thr Trp Glu Ile Asn Asp Leu Lys Gln Lys Glu

180 185 190

Glu Val Leu Arg Asp Glu Leu Leu Lys Ala Gln Gln Ala His Phe Gln

195 200 205

Leu Lys Glu Lys Lys Glu Arg Lys Glu Asn Leu Leu Lys Arg Tyr Pro

210 215 220

Asn Ile Ser Gly Ala Glu Leu Asn Lys Leu Ile Gln Asp Lys Glu Ser

225 230 235 240

Pro Tyr His Ser Phe Trp Lys Lys Asn Asn Pro His Arg Phe Ser Lys

245 250 255

Lys Gly Leu Val Phe Phe Ile Cys Leu Phe Leu Ser Lys Glu Gln Ala

260 265 270

Asn Leu Phe Leu Ser Ser Ile Ser Gly Phe Lys Arg Thr Asp Ala Ser

275 280 285

Tyr Phe Trp Ala Val Arg Ala Met Tyr Met His His Cys Cys His Leu

290 295 300

Pro Gln Pro Arg Leu Glu Ser Ser Asp Met Leu Leu Asp Ile Leu Asn

305 310 315 320

Glu Leu Asn Arg Cys Pro Lys Val Leu Tyr Asn Leu Leu Ser Glu Thr

325 330 335

Asn Arg Ala Tyr Phe Glu Lys Asp Ile Asn Tyr Lys Glu Gly Ser Val

340 345 350

Leu Gln Thr Asp Glu Glu Gly Asn Leu Val Thr Ile Gln Lys Met Leu

355 360 365

Arg His Gln Asp Arg Leu Pro Tyr Phe Ile Leu Lys Tyr Leu Asp Glu

370 375 380

Thr Asn Ala Phe Pro Asp Leu Arg Phe Gln Ile Tyr Leu Gly Lys Leu

385 390 395 400

Val Thr Asp Val Tyr Lys Lys Pro Lys Met Leu Glu Lys Asn Glu Leu

405 410 415

Gly Glu Leu Val Glu Gln Asp Gly Gln Arg Leu Ile Leu Lys Glu Val

420 425 430

His Ala Phe Gly Lys Pro Ser Asp Phe Ala Asp Lys Ile Asn Leu Pro

435 440 445

Lys Glu Leu Glu Val Asn Glu Ile Gln Asp Lys Tyr Gly Glu Met Lys

450 455 460

Gln Glu Glu Leu Lys Val Gly Thr Ile Val Gln Phe Ser Pro Gln Tyr

465 470 475 480

His Ile Ser Ser Asn Arg Ile Ala Leu Lys Leu Phe Lys Thr Lys Asp

485 490 495

Gly Lys Phe Tyr Ser Glu Lys Pro Asp Asn Asp Lys Gln Ala Arg Leu

500 505 510

Ile Ile Leu Asn Lys Asn Trp Phe Trp Phe

515 520

<210> 8

<211> 873

<212> PRT

<213> 宏基因组(metagenomic)

<400> 8

Met Gly Ile Asp Tyr Ser Leu Thr Ser Asp Cys Tyr Arg Gly Ile Asn

1 5 10 15

Lys Ser Cys Phe Ala Val Ala Leu Asn Ile Ala Tyr Asp Asn Cys Asp

20 25 30

His Lys Gly Cys Arg Thr Leu Leu Ser Glu Val Leu Arg Ser Lys Gly

35 40 45

Gly Ile Ser Asp Glu Gln Ile Lys Ser Gln Val Val Asp Gly Ile Gln

50 55 60

Lys Arg Leu Lys Asp Ile Arg Asn Tyr Phe Ser His Tyr Tyr His Ala

65 70 75 80

Glu Asp Cys Leu Arg Phe Gly Asp Gln Asp Ala Val Lys Val Phe Leu

85 90 95

Glu Glu Ile Tyr Lys Asn Ala Glu Ser Lys Thr Val Gly Ala Thr Lys

100 105 110

Glu Ser Asp Tyr Lys Gly Val Val Pro Pro Leu Phe Glu Leu His Asn

115 120 125

Gly Thr Tyr Met Ile Thr Ala Ala Gly Val Ile Phe Leu Ala Ser Phe

130 135 140

Phe Cys His Arg Ser Asn Val Tyr Arg Met Leu Gly Ala Val Lys Gly

145 150 155 160

Phe Lys His Thr Gly Lys Glu Gln Leu Ser Asp Gly Gln Lys Arg Asp

165 170 175

Tyr Gly Phe Thr Arg Arg Leu Leu Ala Tyr Tyr Ala Leu Arg Asp Ser

180 185 190

Tyr Ser Val Gly Ala Glu Asp Lys Thr Arg Cys Phe Arg Glu Ile Leu

195 200 205

Ser Tyr Leu Ser Arg Val Pro Gln Leu Ala Val Asp Trp Leu Asn Glu

210 215 220

Gln Gln Leu Leu Thr Pro Glu Glu Lys Glu Ala Phe Leu Asn Gln Pro

225 230 235 240

Ala Glu Asp Glu Gly Gly Asp Ile Ser Asp Ser Ser Ser Ser Asp Lys

245 250 255

Asn Lys Lys Ser Lys Glu Lys Arg Arg Ser Leu Arg Arg Asp Glu Lys

260 265 270

Phe Ile Leu Phe Ala Ile Gln Phe Ile Glu Gly Trp Ala Ala Glu Gln

275 280 285

Gly Leu Asp Val Thr Phe Ala Arg Tyr Gln Lys Thr Val Glu Lys Ala

290 295 300

Glu Asn Lys Asn Gln Asp Gly Lys Gln Ala Arg Ala Val Gln Leu Lys

305 310 315 320

Tyr Arg Asn Gln Gly Leu Asn Pro Asp Phe Asn Asn Glu Trp Met Tyr

325 330 335

Tyr Ile Gln Asn Glu His Ala Ile Ile Gln Ile Lys Leu Asn Asn Lys

340 345 350

Lys Ala Val Ala Ala Arg Ile Ser Glu Asn Glu Leu Lys Tyr Leu Val

355 360 365

Leu Leu Ile Phe Glu Glu Lys Gly Asn Asp Ala Val Gln Lys Leu Asn

370 375 380

Cys Tyr Ile Tyr Ser Met Ser Gln Lys Ile Glu Gly Glu Trp Lys His

385 390 395 400

Arg Pro Glu Asp Glu Arg Trp Met Pro Ser Phe Thr Lys Arg Ala Asp

405 410 415

Arg Thr Val Thr Pro Glu Ala Val Gln Ser Arg Leu Ser Tyr Ile Arg

420 425 430

Lys Gln Leu Gln Glu Thr Ile Glu Lys Ile Gly Gln Glu Glu Pro Arg

435 440 445

Asn Asn Lys Trp Leu Ile Tyr Lys Gly Lys Lys Ile Ser Met Ile Leu

450 455 460

Lys Phe Ile Ser Asp Ser Ile Arg Asp Ile Gln Arg Arg Pro Asn Val

465 470 475 480

Lys Gln Tyr His Ile Leu Arg Asp Ala Leu Gln Arg Leu Asp Phe Asp

485 490 495

Gly Phe Tyr Lys Glu Leu Gln Asn Tyr Val Asn Asp Gly Arg Ile Ala

500 505 510

Val Ser Leu Tyr Asp Gln Ile Lys Gly Val Asn Asp Ile Ser Gly Leu

515 520 525

Cys Lys Lys Val Cys Glu Leu Thr Leu Glu Arg Leu Ala Gly Leu Glu

530 535 540

Ala Lys Asn Gly Ser Glu Leu Arg Arg Tyr Ile Gly Leu Glu Ala Gln

545 550 555 560

Glu Lys His Pro Lys Tyr Gly Glu Trp Asn Thr Leu Gln Glu Lys Ala

565 570 575

Lys Arg Phe Leu Glu Ser Gln Phe Ser Ile Gly Lys Asn Phe Leu Arg

580 585 590

Lys Met Phe Tyr Gly Asp Cys Cys Gln Lys Arg Cys Phe Asp Glu Glu

595 600 605

Lys Gly Tyr Asn Thr Gln Ala Lys Glu Arg Lys Ser Leu Tyr Ser Ile

610 615 620

Val Lys Glu Lys Leu Lys Asp Ile Lys Pro Ile His Asp Asp Arg Trp

625 630 635 640

Tyr Leu Ile Asp Arg Asn Pro Lys Asn Tyr Asp Asn Lys His Ser Arg

645 650 655

Ile Ile Arg Gln Met Cys Asn Thr Tyr Ile Gln Asp Val Leu Cys Met

660 665 670

Lys Met Ala Met Trp His Tyr Glu Lys Leu Ile Ser Ala Thr Glu Phe

675 680 685

Arg Asn Lys Leu Glu Trp Asn Cys Ile Gly Gln Gly Asn Met Gly Tyr

690 695 700

Glu Arg Tyr Ser Leu Trp Tyr Lys Thr Gly Cys Gly Val Val Ile Gln

705 710 715 720

Phe Thr Pro Ala Asp Phe Leu Arg Leu Asp Ile Ile Glu Lys Pro Ala

725 730 735

Met Ile Glu Asn Ile Cys Gln Cys Phe Val Leu Gly Asn Lys Lys Leu

740 745 750

Asn Ser Gly Ala Glu Lys Lys Ile Thr Trp Asp Lys Phe Asn Lys Asp

755 760 765

Gly Ile Ala Lys Tyr Arg Lys Arg Gln Ala Glu Ala Val Arg Ala Ile

770 775 780

Phe Ala Phe Glu Glu Gly Leu Lys Ile Gln Glu Asp Lys Trp Ser His

785 790 795 800

Glu Arg Tyr Phe Pro Phe Cys Asn Ile Leu Asp Glu Ala Val Lys Gln

805 810 815

Gly Lys Ile Lys Asp Thr Gly Lys Asp Lys Glu Ala Leu Asn Arg Gly

820 825 830

Arg Asn Asp Phe Phe His Glu Glu Phe Lys Ser Thr Glu Asp Gln Gln

835 840 845

Ala Ile Phe Gln Lys Tyr Phe Pro Ile Val Glu Arg Lys Asp Asp Thr

850 855 860

Lys Lys Arg Arg Asp Lys Lys Gln Lys

865 870

<210> 9

<211> 533

<212> PRT

<213> 宏基因组(metagenomic)

<400> 9

Met Ser Gly Leu Pro Leu Pro Lys Pro Leu Arg Arg Trp Leu Leu Gly

1 5 10 15

Thr Leu Ser Asn Gln Glu Arg Lys Ala Gln Asp Trp Leu Gln Ser Lys

20 25 30

Leu Glu Glu Asn Leu Arg Leu Leu Leu Glu Ala Lys Arg Gln Leu Arg

35 40 45

Lys Leu Ala Glu Pro Pro Asn Arg Pro Phe Leu Arg Ala Gly Asp Leu

50 55 60

Ala Thr Trp Ile Ala Arg Asp Ile Ala Tyr Trp Gln His Pro Asp Gly

65 70 75 80

Arg Gln Leu Asn Ser Glu Glu Tyr Gly Glu Leu Gln Ala Ala Leu Ala

85 90 95

Phe Phe Gly Arg Glu Gly Ala Glu Gln Val Tyr Glu Leu Phe Glu Lys

100 105 110

Leu Glu Leu Val Gly Gln Pro Asn Arg Asp His Pro Phe Leu Phe Arg

115 120 125

Leu Lys Asn Arg Met Cys Pro Ala Arg Ser Arg Lys Arg Gln Pro Lys

130 135 140

Pro Ile Arg Gly Ile Leu Glu Phe Tyr Ile Ala Tyr Leu Arg Ala Arg

145 150 155 160

Glu Lys Phe Leu Thr Arg Leu Gln Gly Arg Leu Lys Ser Gly Ser Asn

165 170 175

Pro Thr Pro Glu Met Glu His Trp Ile Pro Pro Leu Arg Ser Arg Ser

180 185 190

Leu Pro Pro Arg Met Asn Glu Arg Ala Val Val Leu Pro Arg Gly Leu

195 200 205

Phe Arg Arg Arg Phe Val Glu Leu Leu Pro Pro Pro Asp Gly Ser Pro

210 215 220

Ala Asp Thr Pro Leu Glu Gln Leu Thr Leu Met Lys Leu Leu Glu Pro

225 230 235 240

Tyr Leu Asp Thr Pro Gln Ala Phe Tyr Arg Tyr Glu Arg Arg Tyr Ala

245 250 255

Arg Asn Glu His Gln Tyr Thr Thr Asp Gly Cys Asn Leu Ser Glu Leu

260 265 270

Ile Pro Gln Ile Glu Asn Glu Ala Thr Gln Gly Ser Glu Glu Glu Asp

275 280 285

Pro Asp Phe Arg Arg Lys Asp Leu Leu Cys Leu Ala Arg Thr Ile Asn

290 295 300

Asp Thr Glu Arg Arg Leu Arg Arg Tyr Gln Leu Gln Asp Arg Leu Leu

305 310 315 320

Trp Met Ile Val Arg Gln Glu Leu Glu Arg Glu Lys Asn Met Asn Leu

325 330 335

Gln Gly Trp Thr Leu Glu Asn Val Gly Phe Asp Gln Pro Asp Ser Pro

340 345 350

Leu Lys Gln Glu Ile Thr Phe Gln Leu Glu Tyr Pro Leu Glu Gly Arg

355 360 365

Asn Glu Pro Ile Arg Ile Glu Ala Lys Leu Arg Pro Arg His Tyr Gly

370 375 380

Lys Met Arg Arg Thr Leu His Asp Arg Arg Leu Pro Gly Leu Leu Gln

385 390 395 400

Trp Leu Asn Thr Glu Ser Val Asp Trp Glu Arg Leu Gln Gln Glu Leu

405 410 415

Thr Lys Thr Ile Thr Arg Gln Asp Glu Val Leu Gln Leu Ile Phe Glu

420 425 430

Leu Glu Lys Ala Val His Ser Arg Phe Glu Lys Glu Cys Thr Ala Lys

435 440 445

Lys Asn Arg Asp Tyr Leu Lys His Arg Asn Tyr Leu Glu Val Leu Arg

450 455 460

Glu His Gln Ile Leu Arg Ser Asp Glu Glu Phe Glu His Leu Leu Asn

465 470 475 480

Leu Arg Asn Arg Phe Ala His Asn Glu Val Pro Ser Pro Ala Glu Leu

485 490 495

Pro Leu Trp Lys Asp Glu Arg Pro Gly Asn Gly Asn Leu Met Glu Trp

500 505 510

Leu Ile Glu Lys Ser Leu Gln Leu Tyr Arg Asp Leu Leu Glu Arg Val

515 520 525

Gln Gly Lys Gly Asp

530

<210> 10

<211> 841

<212> PRT

<213> 宏基因组(metagenomic)

<400> 10

Met Pro Val Asn Tyr Ser Leu Asp Gln Asp Tyr Tyr Lys Gly Thr His

1 5 10 15

Lys Ser Cys Phe Thr Val Pro Leu Asn Ile Ala Trp Asp Asn Gly Ser

20 25 30

Lys Lys Gly Cys Glu Asn Leu Leu Lys Glu Ala Met Arg Thr Arg Gly

35 40 45

Gly Phe Thr Gln Glu Asp Ile Glu Lys Val His Arg Ser Leu Ala Glu

50 55 60

Lys Leu Asn Gly Ile Arg Asp Tyr Phe Ser His Tyr Tyr His Glu Asp

65 70 75 80

Lys Pro Leu Glu Phe Lys Lys Gly Asp Asp Asp Ala Val Lys Asp Phe

85 90 95

Leu Glu Lys Thr Phe Ser Tyr Ala Ala Gly Glu Thr Gln Lys Arg Val

100 105 110

Lys Glu Ser Gly Tyr Gln Gly Ile Ile Pro Pro Ile Phe Glu Leu Cys

115 120 125

Gly Asp Gln Val Arg Ile Thr Ala Ala Gly Val Ile Phe Leu Ala Ser

130 135 140

Phe Phe Val Pro Arg Ser Thr Leu Glu Arg Met Phe Gly Ala Val Gln

145 150 155 160

Gly Phe Lys Arg Ser Asp Arg Gly Asp Leu Asp Thr Gly Gln Lys Arg

165 170 175

Asp Tyr Tyr Phe Thr Arg Ser Leu Leu Ser Phe Tyr Thr Leu Arg Asp

180 185 190

Ser Tyr Tyr Leu Gln Ala Asp Glu Thr Arg Pro Phe Arg Glu Ile Leu

195 200 205

Ser Tyr Leu Ser Cys Val Pro Phe Asp Ser Val Gln Trp Leu Gln Ala

210 215 220

His Gly Lys Leu Ser Lys Ser Glu Glu Lys Glu Phe Phe Gly Arg Pro

225 230 235 240

Val Glu Glu Gln Asp Glu Glu Asn Pro Ala Gln Thr Glu Lys Gln Thr

245 250 255

Ala Pro Ala Gly Arg Arg Met Arg Lys Lys Asn Lys Phe Ile Leu Phe

260 265 270

Ala Val Arg Phe Ile Glu Ala Trp Ala Arg Asn Glu Lys Leu Ser Val

275 280 285

Glu Phe Gly Arg Tyr Arg Asn Ile Gln Asn Glu Glu Asp Arg Arg Lys

290 295 300

Gln Ser Gly Lys Lys Val Arg Glu Val Phe Phe Pro Ser Ala Leu Asn

305 310 315 320

Asn Leu Ser Ala Glu Glu Gln Asp Leu Glu Gly Leu Leu Tyr Ile Arg

325 330 335

Asn Asn His Ala Leu Ile Arg Ile His Leu Lys Ala Lys Thr Pro Val

340 345 350

Thr Val Arg Ile Ser Glu His Glu Leu Met Tyr Leu Val Leu Ala Ile

355 360 365

Leu Ser Gly Lys Gly Gly Asn Ala Val Gln Lys Leu Ser Lys Tyr Val

370 375 380

Trp Asp Val Arg Met Arg Ser Arg Gly Pro Leu Thr Asn Met Pro Arg

385 390 395 400

Asn Phe Pro Ala Phe Leu Arg Ser Pro Ala Ser Glu Val Ser Glu Gln

405 410 415

Ala Val Gln Asn Arg Leu Asn Tyr Ile Arg Lys Thr Leu Lys Glu Ile

420 425 430

Gln Ala Asn Leu Gln Lys Glu Ala Gln Thr Gly Gln Trp Ile Leu Asp

435 440 445

Lys Gly Gln Lys Ile Arg His Ile Leu Arg Phe Ile Ser Asp Ser Met

450 455 460

Pro Asp Phe Arg Arg Arg Pro Ser Val Lys Glu Tyr Asn Glu Leu Arg

465 470 475 480

Glu Leu Leu Gln Thr Leu Ala Phe Asp Asp Phe Tyr Arg Lys Leu Ala

485 490 495

Ser Phe Gln Thr Glu Arg Lys Leu Asp Ala Ala Val Trp Asn Asn Leu

500 505 510

Ala Gln Cys Lys Ser Ile Asn Glu Leu Cys Glu Arg Cys Cys Gln Leu

515 520 525

Gln Gln Gln Arg Leu Asp Glu Leu Glu Lys Gln Gly Gly Asp Glu Leu

530 535 540

Lys Arg Tyr Ile Gly Leu Leu Pro Lys Glu Lys Gly Lys His Tyr Glu

545 550 555 560

Glu Gln Asn Thr Pro Ala Arg Lys Phe Glu Arg Phe Ile Glu Asn Gln

565 570 575

Leu Ser Val Pro Lys Tyr Phe Leu Arg Cys Lys Leu Phe Val Thr Gly

580 585 590

Gly Ser Arg Arg Thr Asn Leu Leu Lys Leu Val Gln Glu His Leu Lys

595 600 605

Pro Lys Thr Ser Val Phe His Glu Glu Arg Leu Tyr Leu Arg Glu Glu

610 615 620

Gln Pro Gly Asp Tyr Pro Trp Ser Asp Arg Lys Ile Ile Gln Lys Met

625 630 635 640

Tyr Tyr Leu Tyr Val Gln Asp Leu Leu Cys Met Gln Met Ala Gln Trp

645 650 655

His Tyr Glu His Leu Thr Pro Gln Val Lys Gly Lys Ile Asp Trp Glu

660 665 670

Ile Asn Ser Glu Ser Lys Glu Ser Asp Gly Tyr Asn Arg Phe Lys Val

675 680 685

Glu Tyr Lys Gly Pro Gln Gly Cys Arg Ile Ile Phe Arg Val Gln Asp

690 695 700

Phe Gly Arg Leu Asp Phe Leu Asn Lys Ala Pro Met Leu Asp Asn Ile

705 710 715 720

Cys Gln Trp Phe Leu Ser Gly Arg Lys Glu Ile Thr Trp Pro Glu Phe

725 730 735

Leu Arg Asp Gly Leu Gln Arg Tyr Arg Gln Arg Gln Ile Leu Val Val

740 745 750

Arg Ala Leu Phe Arg Phe Glu Glu Asn Leu Lys Ile Pro Glu Glu Glu

755 760 765

Trp Lys Gly Lys Ser His Leu Ser Phe Asp Glu Val Leu Glu Arg Phe

770 775 780

Ser Gly Lys Asn Arg Leu Ser Glu Glu Glu Lys Glu Ser Ile Arg Arg

785 790 795 800

Val Arg Asn Asp Phe Phe His Glu Glu Phe Glu Ala Thr Pro Ser Gln

805 810 815

Trp Arg Asp Phe Glu Arg Arg Met Ser Glu Tyr Leu Asn Lys Glu Lys

820 825 830

Arg Glu Lys Pro Lys Lys Lys Lys Arg

835 840

<210> 11

<211> 840

<212> PRT

<213> 宏基因组(metagenomic)

<400> 11

Met Arg Glu Asn Thr Tyr Ser Lys Gln Gln Lys Ser Lys Pro Lys Thr

1 5 10 15

Thr Thr Asp Tyr Gln Gly Ala Asp Lys Trp Cys Phe Ala Ile Val Leu

20 25 30

Asn Arg Ala Cys Asp Asn Tyr Lys Asp Asn His His Leu Phe Pro Glu

35 40 45

Ser Leu Leu Arg Tyr Glu Ser Val Asn Arg Arg Asn Trp Phe Glu Glu

50 55 60

Glu Ile Arg Thr Leu Ile Val Lys Met Asp Thr Glu Asp Pro Ser Leu

65 70 75 80

Arg Pro Arg Asp Pro Lys Lys Val Ile Ala Gly Ile Asn Tyr Ser Pro

85 90 95

Val Asn Arg Arg Leu Asn Asp Val Arg Asn Tyr Phe Ser His His His

100 105 110

His Glu Pro Ser Pro Leu Glu Phe Leu Lys Asp Asp Pro Ile Arg Lys

115 120 125

Ile Met Glu Lys Ala Tyr Glu Lys Ala Lys Phe Glu Glu Ile Lys Arg

130 135 140

Gln Tyr Arg Glu Pro Ser Ile Thr Phe Pro Asp Leu Phe Asp Ala Lys

145 150 155 160

Asn Gln Ile Thr Pro Ala Gly Ile Ile Phe Phe Ala Ser Phe Phe Val

165 170 175

Glu Arg Arg Ile Leu Ala Arg Leu Met Gly Ser Ile Gly Gly Phe Thr

180 185 190

Lys Thr Glu Gly Glu Tyr Asn Ile Thr Arg Asp Ile Phe Thr Thr Tyr

195 200 205

Cys Leu Lys Asp Ser Tyr Ser Ile His Thr Gln Asp Ala Lys Asp Val

210 215 220

Leu Phe Arg Asp Ile Ile Gly Tyr Leu Ser Arg Val Pro Ser Glu Tyr

225 230 235 240

Tyr Lys Tyr Asn Lys Asp His Cys Asp Lys Glu Gly Val Pro Glu Arg

245 250 255

Lys Thr Asp Lys Phe Ile Thr Phe Ala Leu Gln Tyr Leu Glu Ala Phe

260 265 270

Ala Ala Lys Glu Ala Lys Glu Tyr Thr Ile Ser Val Gly Arg Met Glu

275 280 285

Ile Val Arg Glu Glu Arg Lys Glu Ile Val Lys Gly Asp Lys Thr Phe

290 295 300

Lys Pro Cys Pro Asn Lys Gly Lys Ile Lys Val Leu Phe Asp Thr Gly

305 310 315 320

Ala Asn Asn Leu Pro Tyr Tyr Ile Asn His Asn Thr Ala Ile Leu Gln

325 330 335

Phe Gln Lys Asn Gly Gly Ser Arg Tyr Ser Cys Lys Ile Gly Ile Asn

340 345 350

Glu Leu Lys Tyr Leu Val Leu Leu Cys Ile Gln Gly Lys Thr Lys Glu

355 360 365

Ala Ala Glu Ala Leu Tyr His Tyr Leu Asp Glu Ile Gln Glu Arg Phe

370 375 380

Lys Asp Pro Ala Thr Lys Val Gly Ala Asp Asp Ser Glu Arg Phe Lys

385 390 395 400

Gln Arg Leu Pro Gly Phe Ile Lys Leu His Ser Asn Leu Glu Val Ala

405 410 415

Asp Arg Asp Ala Ala Ile Lys Ala Arg Tyr Asp Tyr Ile Ile Asn Lys

420 425 430

Trp Asn Lys Lys Arg Gly Thr Gly Asn Glu Ile Met Ala Asp Ala Lys

435 440 445

Leu His Gln Lys Ala Arg Asp Ile Leu Arg Tyr Ile Lys Trp His Ile

450 455 460

Ser Arg Pro Leu Ser Thr Gly Glu Tyr Asn Phe Leu Leu Ala Leu Leu

465 470 475 480

Ile Glu Lys Asn Phe Asp Lys Phe Asp Glu Arg Ile Thr Glu Leu Trp

485 490 495

Arg Thr Glu Arg Ile Thr Gln Glu Ile Leu Gly Gln Leu Arg Gly Asn

500 505 510

Lys Ser Leu Asp Met Leu Tyr Glu Lys Val Trp Ser Tyr Val Arg Asn

515 520 525

Glu Leu Gln Thr Leu Lys Glu Asn Asp Ile Ser Lys Leu Ala Glu Tyr

530 535 540

Ile Gly Val Val Arg Thr Pro Ala Glu Asn Ala Pro Thr Tyr Glu Thr

545 550 555 560

Lys Val Lys Thr Phe Ala Ala Gln Pro Met Ile Cys Lys Gly Tyr Leu

565 570 575

Arg Glu Gln Phe Phe Gly Thr Glu Lys Thr Phe Ala Lys Leu Val Glu

580 585 590

Asn Gln Leu Gly Thr Asn Pro Asp Val Pro Leu Gly Lys Gln Phe Tyr

595 600 605

His Val Ser Ser Leu Asp Gln Phe Ser Lys Asp Asn Ala Val Leu Tyr

610 615 620

Glu Thr Leu Ala Leu Asp Arg Leu Cys Val Met Met Ala Arg Lys Cys

625 630 635 640

Phe Ala Asp Ile Asn Ala Asn Leu Arg Thr Lys Gly Gln Glu Leu Arg

645 650 655

Trp Lys Asp Asn Asp Gly Lys Glu Leu Ile Glu Ile Asp Ile Pro Arg

660 665 670

Glu Asn Asn Ile Ser Thr Ala Arg His Val Ser Pro Arg Gly Phe Gly

675 680 685

Glu Thr Leu Lys Ile Pro Gln Thr Ser Ala Thr Arg Asp Val Phe Thr

690 695 700

Ile Arg Phe Asp Arg Lys His Tyr Thr Lys Leu Tyr Val Lys Asp Asp

705 710 715 720

Ala Glu Phe Leu Ala Gly Leu Met Lys Tyr Phe Phe Pro Gly Glu Thr

725 730 735

Val Lys Asp Tyr His Leu Leu Tyr Ser Asp Gly Ile Asn His Tyr Thr

740 745 750

Glu Leu Gln Arg Gln Ala Ile Thr Ser Leu Phe Glu Leu Glu Gly Glu

755 760 765

Ile Ile Glu Arg Ala Lys Met Asn Lys Ser Lys His Tyr Ile Arg Phe

770 775 780

Gly Asp Ile Met Lys Lys Ser Glu Phe Lys Pro Asn Glu Gln Asp Ala

785 790 795 800

Val Thr Asn Val Arg Asn Ser Leu Leu His Tyr Glu Leu Lys Phe Thr

805 810 815

Val Lys Asp Phe Arg Ile Phe Val Gln Val Met Asn Arg Glu Lys Met

820 825 830

Asn Lys Asp Trp Val Leu Lys Val

835 840

<210> 12

<211> 840

<212> PRT

<213> 宏基因组(metagenomic)

<400> 12

Met Ala Val Asp Tyr Ser Leu Lys Gln Pro Phe Tyr Gln Gly Val His

1 5 10 15

Lys Ser Cys Phe Thr Val Pro Leu Asn Ile Ala Ala Asp Asn Cys Lys

20 25 30

Gln Lys Gly Tyr Arg Asn Leu Leu Lys Glu Ala Gln Arg Ser Lys Gly

35 40 45

Gly Leu Ser Asp Gln Ser Ile Gln Glu Ala Ala Asp Leu Ile Glu Lys

50 55 60

Arg Leu Ser Ala Ile Arg Asn Tyr Phe Ser His Thr Tyr His Thr Asp

65 70 75 80

Ser Val Leu Thr Phe Gln Lys Glu Asp Pro Val Lys Lys Phe Leu Glu

85 90 95

Thr Ala Trp Ser Tyr Ala Val Ser Glu Thr Gln Lys Asp Ile Ala Glu

100 105 110

Ser Asp Tyr Thr Gly Ile Val Pro Pro Leu Phe Glu Asp Lys Glu Gly

115 120 125

Gln Phe Gln Ile Thr Ala Ala Gly Val Ile Phe Leu Met Ser Phe Phe

130 135 140

Cys His Arg Ser Val Leu Asn Arg Met Phe Gly Ser Val Lys Gly Leu

145 150 155 160

Lys Arg Ser Asp Arg Glu Gln Met Gly Thr Gly Glu Lys Arg Asp Tyr

165 170 175

Gln Phe Thr Arg Lys Leu Leu Ser Phe Tyr Ser Leu Arg Asp Ser Tyr

180 185 190

Ala Val Lys Ala Glu Ala Thr Arg Pro Phe Arg Glu Ile Leu Ser Tyr

195 200 205

Leu Ser Cys Val Pro His Glu Ser Leu Val Trp Leu Ser Ala Arg Gly

210 215 220

Lys Leu Thr Glu Lys Glu Lys Lys Ala Phe Arg His Phe Leu Asp Pro

225 230 235 240

Thr Val Pro Lys Glu Ala Leu Ser Glu Glu Ser Ala Gly Asp Gly Ser

245 250 255

Asp Ser Glu Arg Pro Gly Val Arg Lys Asn Asn Lys Phe Leu Leu Phe

260 265 270

Ala Val Gln Phe Ile Glu Ala Trp Ser Arg Lys Glu Lys Lys Gly Leu

275 280 285

Glu Phe Ala Arg Tyr Arg Lys Ser Arg Val Glu Ala Pro Gly Glu Asn

290 295 300

Gln Asp Gly Ser Glu Lys Arg Ile Val Arg Phe Arg Ser Glu Ile Arg

305 310 315 320

Asp Thr Gln Glu Asp Trp Pro Tyr Tyr Leu Arg Asn Asn His Ala Leu

325 330 335

Leu Arg Leu His Pro Gly Glu Asn Lys Glu Pro Val Asp Ala Arg Ile

340 345 350

Gly Glu Tyr Glu Leu Leu Tyr Leu Val Leu Ala Ile Phe Asp Gly Lys

355 360 365

Gly Ala Lys Ala Ile Gln Lys Leu Ala Asn Tyr Ile Phe Glu Ala Lys

370 375 380

Lys Gln Ile Gln Asn Ala Arg Val Tyr Asp Arg Tyr Gln Asp Leu Leu

385 390 395 400

Pro Ser Phe Leu Thr Ala Gly Asn Lys Pro Val Ser Ala Glu Thr Ile

405 410 415

Arg Asn Arg Leu Ala Tyr Ile Arg Gly Glu Leu Glu Lys Met Leu Glu

420 425 430

Ala Val Gln Lys Glu Lys Lys Ser Gly Arg Trp Glu Met His Lys Gly

435 440 445

Lys Lys Ile Gly His Ile Leu Arg Phe Leu Ser Asn Ser Ile Asp Asp

450 455 460

Ile Arg Arg Arg Pro Asn Val Lys Glu Tyr Asn Arg Leu Arg Asp Leu

465 470 475 480

Leu Gln Gln Leu Gln Trp Asp Glu Phe Asp Lys Ala Leu Gln Ser Tyr

485 490 495

Val Asn Glu Lys Leu Leu Asp Glu Thr Val Tyr Arg Gln Leu Arg Gly

500 505 510

Phe His Ser Leu Asp Glu Leu Phe Glu Arg Cys Cys Arg Leu Glu Leu

515 520 525

Lys Arg Leu Glu Asp Met Glu Lys Ala Gly Gly Asp Arg Leu Asn Arg

530 535 540

Tyr Ile Gly Leu Glu Pro Lys Gly Lys Pro Lys Asn Tyr Ala Asp Leu

545 550 555 560

Asn Thr Leu Gln Lys Lys Gly Glu Arg Phe Leu Lys Gly His Gln Leu

565 570 575

Ser Ile Pro Arg Tyr Phe Leu Arg Asn Ala Leu Tyr Lys Glu Tyr Gln

580 585 590

Ala Thr Glu Glu Arg Lys Pro Thr Ser Leu Tyr Gln Ile Val Arg Glu

595 600 605

Arg Leu Pro Arg Thr Asn Pro Ile Leu Pro Asp Arg Tyr Tyr Leu Leu

610 615 620

Glu Glu Asp Pro Lys Thr Tyr Ser Gly Ser Asp Ser Lys Ile Ile Arg

625 630 635 640

Glu Met Cys Phe Thr Tyr Ile Glu Asp Leu Leu Cys Met Arg Met Ala

645 650 655

Arg Trp His Tyr Glu Gln Leu Ser Glu Lys Leu Arg Lys Lys Leu Gln

660 665 670

Trp Lys Glu Val Gln Thr Gly Pro Ala Gly Tyr Glu Arg Phe Arg Leu

675 680 685

Ile Tyr Lys Ile Ser Asp Glu Leu Ser Ile Glu Phe His Pro Ser Asp

690 695 700

Leu Thr Arg Leu Asp Val Ile Glu Lys Asp Asp Met Leu Thr Asn Ile

705 710 715 720

Ser Gln His Phe Leu Thr Lys Lys Gly Thr Val Arg Trp Thr Glu Phe

725 730 735

Val Ser Gln Gly Met Lys His Tyr Arg Asp Arg Gln Lys Gln Gly Ile

740 745 750

Glu Ala Leu Phe Lys Trp Glu Glu Ser Leu Arg Ile Pro Glu Gly Leu

755 760 765

Trp Lys Glu Glu Gly Tyr Leu Gly Phe Glu Lys Val Leu Glu Glu Ala

770 775 780

Val Lys His Gly Lys Ile Gln Asp Lys Asp Lys Glu Ala Leu Lys Arg

785 790 795 800

Ile Arg Asn Asp Phe Phe His Glu His Phe Cys Gly Thr Pro Ala Asp

805 810 815

Trp Glu Val Phe Lys Arg Val Leu Lys Arg Phe Leu Asn Gln Gly Lys

820 825 830

Asn Glu Lys Lys Arg Phe Lys Lys

835 840

<210> 13

<211> 838

<212> PRT

<213> 宏基因组(metagenomic)

<400> 13

Met Gln Phe Glu Asn Ile Lys Asp Thr Gly Gln Lys Pro Ile Tyr Ser

1 5 10 15

Ile Asp Gln Tyr Glu Gly Ala Lys Lys Trp Cys Phe Ala Ile Val Leu

20 25 30

Asn Arg Ala Cys Asp Asn Tyr Glu Asp Asn Pro Gln Leu Phe Ser Glu

35 40 45

Ser Leu Leu Arg Phe Glu Glu Val Asn Arg Arg Asp Trp Phe Asp Lys

50 55 60

Asp Ile Arg Asp Leu Ile Lys Lys Ala Asp Thr Glu Asp Gln Ile Glu

65 70 75 80

Pro Lys Arg Lys Pro Asn Thr Pro Val Asn Arg Arg Leu His Asp Ile

85 90 95

Arg Asn Tyr Phe Ser His Ser Arg His Gln Asp Asp Cys Leu Tyr Phe

100 105 110

Lys Asn Asp Asp Pro Met Arg Cys Ile Met Glu Ala Ala Tyr Glu Lys

115 120 125

Ala Lys Ile His Ile Lys Gly Arg Gln Thr Glu Gln Ser Asp Ile Pro

130 135 140

Leu Pro Glu Leu Phe Asp Ala Asn Asn Lys Ile Thr Ser Ala Gly Val

145 150 155 160

Leu Phe Leu Ala Ser Phe Phe Val Glu Arg Gly Ile Leu His Arg Leu

165 170 175

Met Gly Asn Ile Gly Gly Phe Lys Asp Asn Arg Gly Lys Tyr Gly Leu

180 185 190

Thr His Asp Ile Phe Thr Thr Tyr Cys Leu Lys Asp Ser Tyr Ser Ile

195 200 205

His Ala Ser Asp Pro Lys Val Val Leu Phe Arg Asp Ile Ala Gly Tyr

210 215 220

Leu Ser Leu Val Ala Cys Glu Tyr Tyr Pro Thr Tyr Leu Ser Lys Ile

225 230 235 240

Pro Lys Glu Asn Ala Gly Glu Lys Ser Ser Asp Glu Glu Lys Tyr Ala

245 250 255

Glu Arg Lys Thr Asp Lys Phe Ile Leu Phe Ala Leu Lys Tyr Leu Glu

260 265 270

Glu Phe Val Leu Pro Ser Leu Lys Asp Asp Tyr Leu Val Asp Ile Gly

275 280 285

Arg Ile Asp Ile Ile Arg Glu Glu Ser Lys Glu Thr Glu Glu Lys Asp

290 295 300

Glu Gln Tyr Lys Pro His Pro Asn Gln Gly Lys Val Lys Val Val Phe

305 310 315 320

Asp Ser Ile Asn Lys Glu Leu Pro Tyr Tyr Ile Asn His Asn Thr Val

325 330 335

Ile Leu Arg Ile Gln Lys Asn Gly Val Met Ala Tyr Ser Cys Lys Ile

340 345 350

Gly Val Asn Asp Leu Lys Tyr Leu Leu Leu Leu Cys Leu Gln Gly Lys

355 360 365

Thr Asp Lys Ala Leu Asp Ala Ile Tyr Asn Tyr Leu His Ser Met Gln

370 375 380

Asp Pro Pro Glu Val Val Lys Ile Gly Ala Thr Asp Lys Leu Phe Gln

385 390 395 400

Gly Leu Pro Glu Phe Ile Leu Lys Gln Ser Gly Ile Lys Val Gln Asp

405 410 415

Lys Asn Lys Glu Lys Ala Ala Arg Ile Lys Tyr Ile Arg Asp Lys Trp

420 425 430

Glu Lys Lys Lys Ser Glu Ser Ala Asp Met Glu Leu His Arg Lys Gly

435 440 445

Arg Asp Ile Leu Arg Tyr Val Asn Trp His Cys Glu Thr Pro Leu Gly

450 455 460

Thr Glu Lys Tyr Asp Gln Leu Leu Val Leu Leu Val Asn Lys Asn Phe

465 470 475 480

Val Val Phe Gly Asp Glu Leu Asn Gln Leu Lys Arg Thr Glu Ile Ile

485 490 495

Ser Lys Asp Ile Leu Glu Lys Leu Ser Gly Phe Gln Thr Ile Asn Thr

500 505 510

Leu His Gln Lys Val Cys Asn Leu Val Leu Glu Glu Leu Ser Ser Leu

515 520 525

Glu Lys Asn Asp Pro Gly Lys Leu Ala Glu His Ile Gly Leu Val Arg

530 535 540

Lys Pro Ala Pro Glu Asn Asn Pro Pro Pro Glu Tyr Lys Glu Lys Val

545 550 555 560

Arg Arg Phe Val Glu Gln Pro Met Ile Tyr Lys Gly Phe Leu Arg Asp

565 570 575

Gln Phe Phe Val Asn Lys Asp Gln Asp Gly Lys Lys Leu Lys Glu Gln

580 585 590

Lys Thr Phe Ala Lys Leu Val Glu Glu Thr Leu Gly Gln Asn Ala Asp

595 600 605

Val Pro Leu Gly Lys Asp Phe Tyr Tyr Val Pro Asn Ile Glu Lys Asp

610 615 620

Glu Lys Lys Asn Arg Phe His Lys Asp Asn Ala Val Leu Tyr Glu Thr

625 630 635 640

Leu Ala Leu Asp Arg Leu Cys Ala Met Met Ala Arg Lys Cys Leu Thr

645 650 655

Gln Ile Asn Lys Asn Leu Ala Glu Lys Ser Glu Glu Ile Asp Trp Arg

660 665 670

Asn Glu Asp Gly Lys Asp Phe Ile Tyr Leu Lys Leu Val Lys Ser Asp

675 680 685

Arg Pro Gln Glu Thr Phe Lys Ile Arg Phe Lys Val Asn Asp Phe Ala

690 695 700

Lys Leu Tyr Val Met Asp Asp Pro Asp Phe Leu Gly Gly Leu Met Lys

705 710 715 720

His Phe Phe Pro Gln Glu His Ser Ile Glu Tyr His Lys Leu Tyr Arg

725 730 735

Asn Gly Ile Glu Arg Tyr Thr Asp Arg Gln Lys Asp Gly Ile Glu Ala

740 745 750

Ile Leu Arg Leu Glu Asp Ser Val Ile Arg Gln Lys Gly Met Lys Pro

755 760 765

Lys Pro Ala Lys Asn Tyr Ile Ser Phe Ser Glu Ile Met Ala Gln Thr

770 775 780

Asp Tyr Pro Glu His Asp Gln Lys Val Leu Asn Lys Val Arg Arg Ala

785 790 795 800

Val Leu His Tyr His Leu Lys Phe Glu Pro Ala Asp Tyr Asn Arg Phe

805 810 815

Val Asp Ile Met Lys Lys Asn Lys Phe Trp Asp Gly Glu Arg Lys Asn

820 825 830

Lys Glu Ser Arg Gly Arg

835

<210> 14

<211> 804

<212> PRT

<213> 宏基因组(metagenomic)

<400> 14

Met Glu Phe Glu Asn Ile Lys Lys Thr Ser Asn Lys Glu Val Tyr Ser

1 5 10 15

Ile Glu Gln Tyr Glu Gly Glu Lys Lys Trp Cys Phe Ala Ile Val Leu

20 25 30

Asn Arg Ala Gln Thr Asn Leu Glu Glu Asn Pro Lys Leu Phe Glu Gln

35 40 45

Thr Leu Thr Arg Phe Glu Lys Ile Met Lys Gln Asp Trp Phe Asn Glu

50 55 60

Glu Thr Lys Lys Leu Ile Tyr Glu Lys Glu Glu Glu Asn Lys Val Lys

65 70 75 80

Glu Glu Ile Gln Ile Ala Ala Ser Glu Arg Leu Lys Asn Leu Arg Asn

85 90 95

Tyr Phe Ser His Tyr Leu His Ala Pro Asp Cys Leu Ile Phe Asn Arg

100 105 110

Asn Asp Thr Ile Arg Ile Ile Met Glu Lys Ala Tyr Glu Lys Ser Arg

115 120 125

Phe Glu Ala Lys Lys Lys Gln Gln Glu Asp Ile Ser Ile Glu Phe Pro

130 135 140

Glu Leu Phe Glu Glu Glu Asp Lys Ile Thr Ser Ala Gly Val Val Phe

145 150 155 160

Phe Val Ser Phe Phe Ile Glu Arg Arg Phe Leu Asn Arg Leu Met Gly

165 170 175

Tyr Val Gln Gly Phe Arg Lys Thr Glu Gly Glu Tyr Asn Ile Thr Arg

180 185 190

Gln Val Phe Ser Lys Tyr Cys Leu Lys Asp Ser Tyr Ser Val Gln Ala

195 200 205

Gln Asp His Asp Ala Val Met Phe Arg Asp Ile Leu Gly Tyr Leu Ser

210 215 220

Arg Val Pro Thr Glu Ile Tyr Gln His Ile Lys Leu Thr Arg Lys Arg

225 230 235 240

Ser Gln Asp Gln Leu Ser Glu Arg Lys Thr Asp Lys Phe Ile Leu Phe

245 250 255

Ala Leu Lys Tyr Leu Glu Asp Tyr Gly Leu Lys Asp Leu Ala Asp Tyr

260 265 270

Thr Ala Cys Phe Ala Arg Ser Lys Ile Lys Arg Glu Asn Glu Asp Thr

275 280 285

Lys Glu Thr Asp Gly Asn Lys His Lys Phe His Arg Glu Lys Pro Val

290 295 300

Val Glu Ile His Phe Asp Lys Glu Lys Gln Asp Gln Phe Tyr Ile Lys

305 310 315 320

Arg Asn Asn Val Ile Leu Lys Ala Gln Lys Lys Gly Gly Gln Ser Asn

325 330 335

Val Phe Arg Met Gly Val Tyr Glu Leu Lys Tyr Leu Val Leu Leu Ser

340 345 350

Leu Leu Gly Lys Ala Glu Glu Ala Ile Gln Arg Ile Asp Arg Tyr Ile

355 360 365

Ser Ser Leu Lys Lys Gln Leu Pro Tyr Leu Asp Lys Ile Ser Asn Glu

370 375 380

Glu Ile Gln Lys Ser Ile Asn Phe Leu Pro Arg Phe Val Arg Ser Arg

385 390 395 400

Leu Gly Leu Leu Gln Val Asp Asp Glu Lys Arg Leu Lys Thr Arg Leu

405 410 415

Glu Tyr Val Lys Ala Lys Trp Thr Asp Lys Lys Glu Gly Ser Arg Lys

420 425 430

Leu Glu Leu His Arg Lys Gly Arg Asp Ile Leu Arg Tyr Ile Asn Glu

435 440 445

Arg Cys Asp Arg Pro Leu Ser Arg Lys Glu Tyr Asn Asn Ile Leu Lys

450 455 460

Phe Ile Val Asn Lys Asp Phe Ala Gly Phe Tyr Asn Glu Leu Glu Glu

465 470 475 480

Leu Lys Arg Thr Arg Arg Leu Asp Lys Asn Ile Ile Gln Lys Leu Ser

485 490 495

Gly His Thr Thr Leu Asn Ala Leu His Glu Arg Val Cys Asp Leu Val

500 505 510

Leu Gln Glu Leu Gly Ser Leu Gln Ser Glu Asn Leu Lys Glu Tyr Ile

515 520 525

Gly Leu Ile Pro Lys Glu Glu Lys Glu Val Thr Phe Arg Glu Lys Val

530 535 540

Asp Arg Ile Leu Glu Gln Pro Val Val Tyr Lys Gly Phe Leu Arg Tyr

545 550 555 560

Glu Phe Phe Lys Glu Asp Lys Lys Ser Phe Ala Arg Leu Val Glu Glu

565 570 575

Ala Ile Lys Thr Lys Trp Ser Asp Phe Asp Ile Pro Leu Gly Glu Glu

580 585 590

Tyr Tyr Asn Ile Pro Ser Leu Asp Arg Phe Asp Arg Thr Asn Lys Lys

595 600 605

Leu Tyr Glu Thr Leu Ala Met Asp Arg Leu Cys Leu Met Met Ala Arg

610 615 620

Gln Tyr Tyr Leu Arg Leu Asn Glu Lys Leu Ala Glu Lys Ala Gln His

625 630 635 640

Ile Tyr Trp Lys Lys Glu Asp Gly Arg Glu Val Ile Ile Phe Lys Phe

645 650 655

Gln Asn Pro Lys Glu Gln Lys Lys Ser Phe Ser Ile Arg Phe Ser Ile

660 665 670

Leu Asp Tyr Thr Lys Met Tyr Val Met Asp Asp Pro Glu Phe Leu Ser

675 680 685

Arg Leu Trp Glu Tyr Phe Ile Pro Lys Glu Ala Lys Glu Ile Asp Tyr

690 695 700

His Lys His Tyr Ala Arg Ala Phe Asp Lys Tyr Thr Asn Leu Gln Lys

705 710 715 720

Glu Gly Ile Asp Ala Ile Leu Lys Leu Glu Gly Arg Ile Ile Glu Arg

725 730 735

Arg Lys Ile Lys Pro Ala Lys Asn Tyr Ile Glu Phe Gln Glu Ile Met

740 745 750

Asn Arg Ser Gly Tyr Asn Asn Asp Gln Gln Val Ala Leu Lys Arg Val

755 760 765

Arg Asn Ala Leu Leu His Tyr Asn Leu Asn Phe Glu Arg Glu His Leu

770 775 780

Lys Arg Phe Tyr Gly Val Val Lys Arg Glu Gly Ile Glu Lys Lys Trp

785 790 795 800

Ser Leu Ile Val

<210> 15

<211> 799

<212> PRT

<213> 宏基因组(metagenomic)

<400> 15

Met Ile Asn Ile Glu Leu Lys Lys Glu Glu Ala Ala Phe Tyr Phe Asn

1 5 10 15

Gln Ala Asn Leu Asn Ile Ser Gly Leu Asp Glu Val Ile Glu Lys Gln

20 25 30

Leu Pro His Ile Gly Ser Lys Lys Glu Asn Ala Lys Lys Ala Ile Asp

35 40 45

Lys Ile Phe Asp Asn Ile Thr Val Leu Lys Lys Val Glu Asn Phe Val

50 55 60

Phe Tyr Phe Lys Asp Val Ala Lys Asn Glu Arg Val Glu Leu Asp Ala

65 70 75 80

Leu Leu Leu Lys Leu Ile Asp Leu Arg Asn Phe Tyr Ser His Tyr Val

85 90 95

His Asn Asp Asn Val Lys Ile Leu Ser Asp Gly Glu Glu Thr Leu Leu

100 105 110

Glu Lys Tyr Tyr Gln Ile Ala Ile Glu Ala Thr Gly Ser Lys Asp Val

115 120 125

Lys Leu Glu Ile Ile Asp Asn Glu Lys Lys Leu Thr Asp Ala Gly Ile

130 135 140

Leu Phe Leu Leu Cys Met Phe Leu Lys Lys Ser Gln Ala Asn Lys Leu

145 150 155 160

Ile Ser Ser Ile Ser Gly Phe Lys Arg Asn Asp Lys Glu Trp Gln Pro

165 170 175

Arg Arg Asn Leu Phe Thr Tyr Tyr Ser Leu Arg Glu Gly Tyr Lys Val

180 185 190

Val Pro Asp Met Glu Lys His Phe Leu Leu Phe Thr Leu Val Asn His

195 200 205

Leu Ser Thr Gln Asp Glu Asn Ile Glu Asn Thr Gln Pro Ser Asp Asp

210 215 220

Ile Gly Arg Gly Leu Phe Phe His Arg Ile Ala Ser Thr Phe Leu Asn

225 230 235 240

Ile Ser Gly Ile Phe Asn Asn Met Glu Phe Tyr Pro Tyr Gln Ser Asn

245 250 255

Arg Leu Lys Glu Arg Arg Gly Asp Ile Ala Pro Asp Lys Asp Ser Phe

260 265 270

Ala Trp Ile Glu Pro Phe Gln Gly Asn Ser Tyr Phe Lys Ile Asn Gly

275 280 285

Tyr Lys Gly Val Ile Gly Glu Asn Glu Leu Lys Glu Leu Cys Phe Ala

290 295 300

Val Leu Leu His Asn Lys Ser Lys Tyr Ala Val Glu Gln Ile Glu Lys

305 310 315 320

Phe Leu Lys Cys Phe Lys Glu Val Gln Ser Lys Gln Glu Ile Ile Glu

325 330 335

Cys Asp Ile Leu Asp Glu Cys Tyr Phe Pro Ala Asn Tyr Leu Asn Gln

340 345 350

Pro Glu Thr Lys Ser Leu Lys Glu Lys Leu Leu Ser Arg Ile Thr Gly

355 360 365

Lys Ile Asn Tyr Ser Phe Asp Thr Ala Glu Lys Ala Phe Asp Lys Met

370 375 380

Lys Asp Val Met Glu Phe Ile Asn Gly Cys Leu Pro Ser Asp Glu Lys

385 390 395 400

Leu Lys Arg Lys Asp Tyr Ser Arg Tyr Leu Lys Met Val Arg Phe Trp

405 410 415

Gly Gly Glu Lys Asp Asn Ile Lys Arg Glu Phe Glu Gly Lys Lys Trp

420 425 430

Thr Arg Phe Phe Pro Ser Glu Leu Trp His Lys Arg Thr Leu Glu Asp

435 440 445

Val Tyr Lys Phe Ala Leu Lys Lys Asn Lys Lys Arg Leu Glu Glu Leu

450 455 460

Lys Val Lys Ile Glu Gly Leu Asn Glu Asp Asp Leu Leu Lys Tyr Gln

465 470 475 480

Lys Val Asn Asn Ile Lys Asn Leu Glu Asn Leu Arg Leu Leu Ala His

485 490 495

Asp Leu Asp Leu Ser Trp Arg Glu Lys Asp Trp Gly Glu Tyr Ser Gly

500 505 510

Gln Ile Lys Lys Gln Ile Ser Asp Asn Gln Lys Leu Thr Ile Met Lys

515 520 525

Gln Arg Val Ile Ala Glu Leu Lys Lys Lys His Gly Ile Glu Asn Ile

530 535 540

Asn Leu Arg Ile Ser Leu Asp Ser Asn Lys Ser Ile Gln Ala Val Leu

545 550 555 560

Asn Arg Ile Ala Ile Pro Lys Gly Phe Ile Lys Arg His Val Leu His

565 570 575

Leu Gln Glu Asn Glu Lys Thr Ser Arg Lys Ile Arg Glu Ala Lys Cys

580 585 590

Lys Ile Leu Leu Ser Lys Lys Tyr Glu Tyr Leu Ser Arg Lys Phe Leu

595 600 605

Asp Glu Lys Asn Phe Asp Lys Leu Thr Gln Ile Asn Gly Leu Tyr Glu

610 615 620

Lys Asn Arg Leu Ile Ala Phe Met Val Ile Tyr Leu Leu Lys Gln Leu

625 630 635 640

Gly Leu Glu Leu Lys Asn Glu Thr Lys Leu Ile Glu Leu Lys Lys Thr

645 650 655

Arg Val Lys Tyr Lys Ile Ser Asp Lys Val Ala Glu Asp Ile Pro Leu

660 665 670

Ser His Tyr Pro Ser Leu Val Tyr Ala Met Ser Arg Lys Tyr Val Asp

675 680 685

Asn Ile Asp Asn Tyr Glu Phe Pro Asp Glu Tyr Ala Lys Lys Ala Ile

690 695 700

Leu Asp Lys Val Asp Ile Ile Glu Asn Gln Arg Met Glu Phe Ile Lys

705 710 715 720

Gln Val Leu Gly Phe Glu Lys Tyr Leu Phe Asp Asn Asn Ile Ile Asp

725 730 735

Lys Ser Lys Phe Thr Asp Val Glu Thr His Ile Ser Phe Val Lys Ile

740 745 750

His Asp Glu Leu Ile Glu Lys Gly Trp Asp Thr Glu Lys Leu Ser Lys

755 760 765

Leu Lys His Ala Arg Asn Lys Ala Leu His Gly Glu Ile Pro Gly Gly

770 775 780

Thr Ser Phe Glu Lys Ala Lys Leu Leu Ile Asn Glu Leu Lys Lys

785 790 795

<210> 16

<211> 650

<212> PRT

<213> 宏基因组(metagenomic)

<400> 16

Met Asp Thr Asn Glu Ala Tyr Thr Ala Tyr Asn Ser Arg Asn Ser Phe

1 5 10 15

Lys Arg Ile Phe Asp Phe Lys Gly Glu Ile Ala Pro Ile Ala Glu Lys

20 25 30

Ala Asn Leu Asn Tyr Asp Ile Lys Ala Lys Asn Ala Ile Asn Arg Glu

35 40 45

Gln Arg Leu His Tyr Phe Thr Val Gly His Thr Phe Lys Asn Ile Asp

50 55 60

Thr Glu His Val Phe Glu Ile Leu Leu Asp Glu Glu Thr Arg Glu Lys

65 70 75 80

Arg Pro Tyr Thr Phe Leu Ser Leu Gln Gln Phe Asn Thr Asp Phe Cys

85 90 95

Thr Ala Ile Lys Glu Val Ile Ser Asn Ile Arg His Ile Asn Ser His

100 105 110

Tyr Ile His Asp Phe Glu Arg Ile Lys Thr Asp Asn Ile Pro Pro Glu

115 120 125

Ile Ile Thr Phe Leu Lys Glu Ser Phe Glu Leu Ala Val Ile Gln Ile

130 135 140

Tyr Leu Lys Glu Asn Asn Ile Thr Tyr Leu Gln Phe Ile Glu Gln Lys

145 150 155 160

Asn Thr Asp Thr Thr Ile Val Lys Tyr Leu His Asp Lys Phe Tyr Ser

165 170 175

Leu Asp Asn Thr Lys Thr Asp Thr Lys Asn Asp Thr Ser Pro Ser Leu

180 185 190

Ala Glu Tyr Ile Ala Phe Arg Asn Thr Phe Lys Thr Leu Ser Lys Glu

195 200 205

Lys Ala Leu Asp Ser Leu Leu Phe Val Thr Val Asp Ala Gly Phe Pro

210 215 220

Trp Lys Leu Glu Glu Thr His Thr Ala Cys Thr Ile Thr Gln Gly Thr

225 230 235 240

Tyr Leu Ser Phe Asn Ala Cys Leu Phe Leu Leu Ser Leu Phe Leu Tyr

245 250 255

Lys Ser Glu Ala Asn Gln Leu Ile Ser Lys Ile Lys Gly Phe Lys Lys

260 265 270

Asn Lys Thr Asp Glu Glu Lys Ser Lys Arg Glu Ile Phe Ser Phe Phe

275 280 285

Ser Lys Lys Phe Ser Ser Gln Asp Ile Asp Ser Glu Glu Asn His Leu

290 295 300

Val Lys Phe Arg Asp Leu Ile Gln Tyr Ile Asn His Tyr Pro Val Glu

305 310 315 320

Trp Asn Lys Asp Leu Lys Leu Glu Ser Gly His Pro Leu Met Thr Asp

325 330 335

Lys Leu Ile Ala Lys Ile Thr Asp Met Glu Ile Asp Arg Ala Tyr Pro

340 345 350

Asp Tyr Ala Gly Asn Asn Lys Phe Gln Ala Tyr Ala Lys Glu Leu Leu

355 360 365

Trp Asn Val Pro Ser Lys Thr Thr Phe Thr Thr Glu Glu Ile Glu Ala

370 375 380

Phe Ala Phe Glu Ile Asn Lys Ser Pro Glu Leu Lys Asp Ala Lys Lys

385 390 395 400

Lys Leu His Asp Leu Gln Ala Lys Met Gly Leu Tyr Gly Phe Lys Lys

405 410 415

Val Lys Asn Glu Gln Glu Ile Ala Lys Thr Ile Lys Arg Ile Lys Trp

420 425 430

Ile Gln Asn Asp Leu Asn Pro Val Thr Glu Asn Val Lys Lys Arg Leu

435 440 445

Ala Gln Phe Ser Leu Tyr Gly Ser Tyr Gly Arg Asn Gln Asp Arg Phe

450 455 460

Met Asp Phe Ala Thr Arg Tyr Leu Ala Glu Gln Lys Tyr Phe Gly Val

465 470 475 480

Asp Ala Glu Phe Lys Met Tyr Lys Tyr Phe Thr Ser Glu Glu Gln Asn

485 490 495

Thr Glu Leu Ala Thr Tyr Glu Leu Pro Lys Asp Lys Lys Ala Tyr Asp

500 505 510

Lys Leu Arg Phe His Lys Gly Lys Leu Val His Phe Ser Thr Phe Glu

515 520 525

Asn His Leu Lys Lys Tyr Glu Ser Trp Asp Thr Pro Phe Val Ile Glu

530 535 540

Asn Asn Ala Ile Gln Val Lys Leu Ser Ile Arg Gln Asp Asn Lys Lys

545 550 555 560

Glu Pro Ile Glu Lys Ile Leu Ser Ile Gln Arg Ala Leu Met Leu Tyr

565 570 575

Phe Leu Glu Asp Ala Leu Phe Gln Thr Gly Asn Asn Asn Ile Ile Glu

580 585 590

Asn Lys Gly Arg Ile Leu Val Glu Gln Tyr Tyr Thr Val Tyr Asn Asn

595 600 605

Asp Phe Val Gln Ser Lys Thr Val Leu Glu Glu Asn Asp Ser Ile Ser

610 615 620

Pro Glu Gln Lys Asn Ala Leu Lys Lys Ile Val Pro Lys Arg Leu Leu

625 630 635 640

His Arg Tyr Phe Ala Arg Ser Asn Lys Leu

645 650

<210> 17

<211> 695

<212> PRT

<213> 宏基因组(metagenomic)

<400> 17

Leu Pro Lys Leu Leu Ala Leu Gln Leu Leu Thr Arg Thr Ala Glu Gln

1 5 10 15

Pro Ile Ile Asn Phe Ile Gln Thr Thr Asn Ala Lys Ile Phe Asn Phe

20 25 30

Gly Glu Leu Glu Lys Ile Lys Asp Arg Thr Asn Tyr Ile Pro Glu Val

35 40 45

Phe Thr Lys Arg Val Val Arg Glu Arg Glu Leu Ile Asn Asn Lys Gly

50 55 60

Gly Ile Thr Tyr Leu Ser Asn Lys Leu Glu Met Asn Ile Leu Asn Lys

65 70 75 80

Tyr His Leu Ser Ile Val Gln Leu Ala Ala Met Asp Lys Asp Ser Phe

85 90 95

Lys Lys Leu Thr Gly Lys Asn Lys Lys Asp Ile Glu Leu Leu Ser Gln

100 105 110

Ile Lys Tyr Gln Tyr Leu Leu Lys Gln Arg Arg Glu Glu Leu Lys Lys

115 120 125

His Leu Pro Thr Gly Leu Asn Leu Asp Met Leu Pro Lys Arg Val Leu

130 135 140

Asp Tyr Leu Met Gln Ser Asn Glu Arg Asn Glu Lys Lys Lys Ile His

145 150 155 160

Gln Lys Ile Lys Asp Ile Lys Asp Glu Ala Lys Leu Leu Asn Lys Ser

165 170 175

Ile Lys Lys Glu Ile Glu Lys Thr Gln Gln Glu Gln Arg Ile Lys Leu

180 185 190

Gly Glu Ile Ala Thr Phe Ile Ala Ser Asp Met Leu Asn Met Ile Ile

195 200 205

Asp Lys Glu Leu Lys Ser Arg Ile Thr Asn Pro Tyr Tyr Asn Lys Leu

210 215 220

Gln Asn Lys Ile Ala Tyr Phe Ser Leu Asn Lys Gly Glu Ile Ile Ser

225 230 235 240

Ile Cys Asp Glu Leu Ser Leu Phe Asp Thr Lys Lys Gly His Val Phe

245 250 255

Leu Thr Arg Asn Leu Ile Phe Lys Ser Thr Gly Ile Ile Asp Phe Tyr

260 265 270

Gln Asn Tyr Leu Glu Ala Lys Ile Ala Trp Ile Asp Asn Lys Leu Phe

275 280 285

Val Lys Gly Lys Met Gly Gly Tyr Thr Ile Pro Asn Asn Asn Val Pro

290 295 300

Tyr Ser Ile Leu Lys Ile Lys Asn Glu Ile Arg Thr Tyr Asn Phe Asn

305 310 315 320

Thr Trp Leu Lys Asn Lys Ile Ile Ser Pro Val Glu Leu Pro Asn Ser

325 330 335

Leu Met Asp Asp Gln Leu Ala Lys Val Leu Lys Ser Ala Leu Lys Lys

340 345 350

Glu Ser Lys Thr Tyr Asn Asp Gly Asp Lys Phe Ser Ile Leu Leu Ala

355 360 365

Lys Tyr Leu Ser Asn Asp Ser Gln Pro Phe Tyr Glu Tyr Thr Arg Asn

370 375 380

Tyr Thr Ile Asn Thr Glu Pro Val Cys Phe Asp Val Gln Gly Leu Ser

385 390 395 400

Ser Lys Asp Ile Gln Asp Lys Tyr Gly Asn Arg Val Ala Glu Asn Glu

405 410 415

Lys Leu Ile Arg Phe Ile Gln Thr Lys Asp Arg Ile Leu Lys Leu Val

420 425 430

Cys Asp Glu Leu Leu Leu Lys Asp Pro Ser Ile Gly Ser Lys Asp Arg

435 440 445

Phe Lys Leu Ser Glu Ile His Pro Thr Ser Leu His Asn Pro Leu Glu

450 455 460

Arg Gln Ala Glu Phe Lys His Lys Ile Ile Arg Lys Gly Thr Glu Val

465 470 475 480

Phe Phe Thr Val Val Ala Lys Asp Thr Lys Glu Gln Ile Gln Glu Val

485 490 495

Ile Glu Tyr Glu Lys Ile Thr Ser Ser Asp Glu Lys Glu Lys Tyr Pro

500 505 510

Gly Gln Lys Trp Tyr Gln Trp Thr Ile Lys Asp Phe Gly Arg Phe Lys

515 520 525

Arg Phe Leu Lys Asp Arg Arg Leu Pro Asn Leu Ser Glu Tyr Phe Glu

530 535 540

Asp Lys Asp Ile Thr Phe Asp Phe Leu Glu Tyr Gln Ile Lys Glu Tyr

545 550 555 560

Asp Lys Tyr Arg Asn Asn Val Phe Asp Leu Thr Phe Glu Leu Glu Lys

565 570 575

Ser Ile Ala Thr Ser Asp Leu Glu Gly Ile Arg Gln Ile Glu Gln Arg

580 585 590

Leu His Ile Arg Pro Lys Gly Phe Phe Glu Ile Glu Phe Asp Val Tyr

595 600 605

Val Glu Trp Leu Asp Arg Lys Gly Ile Gln Tyr Asn Lys Asp Leu Ile

610 615 620

Asn Glu Cys Arg Asn Arg Phe Ser His Ser Glu Phe Pro Lys Tyr Glu

625 630 635 640

His Leu Asn Gln Ile Pro Lys Ile Thr Arg Gln Gln Ile Ile Asp Phe

645 650 655

Glu Tyr Asn Lys Arg Thr Arg Glu Tyr Lys Asn Asn Ala Asp Ile Ser

660 665 670

Ile Ser Glu Lys Ile Thr Asn Gln Tyr Lys Thr Glu Ile Glu Gln Ile

675 680 685

Ile His Gln Ile Arg Leu Gln

690 695

<210> 18

<211> 436

<212> PRT

<213> 宏基因组(metagenomic)

<400> 18

Met Ala His Asp Lys Ile Ile Arg Leu Tyr Arg Asn Thr Leu Cys Val

1 5 10 15

Tyr Ser Asn Ile Ala Ala Asp Asn Ile Asp Lys Trp Leu His Lys Gln

20 25 30

Lys Pro Ser Lys Lys Ala Thr Phe Glu Glu Gln Thr Lys Ile Val Asn

35 40 45

Ala Ala Tyr Lys Asn Ile Tyr Phe Val Glu Trp Ala Ile Asn Asn Gly

50 55 60

Val Phe Phe Leu Pro Glu Ser Asn Asp Ser Ser Glu Gln Glu Lys Ala

65 70 75 80

Ile Lys Glu Asn Tyr Cys Arg Leu Leu Gly Val Ile Gln Leu Leu Asp

85 90 95

Asn Val Arg Asn Tyr Trp Ser His Leu Lys His Asn Lys Thr Ile Ile

100 105 110

Ala Ala Asn Leu Asp Gly Lys Asn Asn Glu Thr Val Lys Asn Lys Ser

115 120 125

Glu Asn Ser Asn Asn Glu Asp Thr Cys Asn Ala Val Leu Ser Gly Leu

130 135 140

Lys Asp Phe Ile Thr Gln Ala Tyr Val Ala Ala Cys Ser Glu Thr Gly

145 150 155 160

Val Lys Ile Pro Asp Lys Tyr Lys Ala Ser Glu Gly Leu Phe Ala Cys

165 170 175

Val Lys Lys Pro Glu Glu Asp Tyr Lys Val Ile Lys Ile Thr Ser Glu

180 185 190

Phe Thr Ile Thr Gly Phe Val Phe Tyr Ile Cys Leu Phe Leu Asp Gly

195 200 205

Gln Gln Ile Asn Glu Phe Leu Glu Ala Met Glu Gln Ser Val Tyr Asn

210 215 220

Phe Asp Glu Leu Ala Asp Arg Gln Asn Phe Arg Leu Thr His Pro Lys

225 230 235 240

Glu Lys Tyr Pro Glu Ser Leu Ser Lys Lys Ile Lys Asp Phe Phe Tyr

245 250 255

Ala Arg Asp Val Tyr Thr Tyr Trp Arg Val Arg Gly His Arg Asp Asn

260 265 270

Ile Leu Glu Asp Lys Thr Leu Asp Glu Lys Glu Asp Tyr Tyr Ser Leu

275 280 285

Leu Glu Tyr Leu Lys Arg Cys Pro Lys Glu Arg Leu Glu Leu Val Glu

290 295 300

Glu Ser Pro Ser Gly Lys Lys Glu Phe Val Ile Asp Asn Val Ser Tyr

305 310 315 320

Asp Val Arg Glu Lys Asn Lys Phe Met Glu Trp Gly Leu Glu Phe Trp

325 330 335

Asp Asn Glu Ile Glu Arg Leu Met Lys Ile Tyr Pro Asp Ser Lys Leu

340 345 350

Asp Met Trp Lys Trp Gly His His Gln Thr Ala Asp Tyr Lys His Asn

355 360 365

Ile Lys Leu Glu Leu Lys Gln Gln Ala Glu Glu Gln Gly Arg Pro Tyr

370 375 380

Gln Phe Pro Arg Tyr Glu Lys Val Ile Phe Asp Leu Pro Glu Thr Glu

385 390 395 400

Glu Glu Arg Arg Asn Tyr Arg Asn Asp Glu His Gly Phe Thr Tyr Tyr

405 410 415

Leu Leu Lys Asp Asp Ser Asn Thr Phe Ser Gln Ala Met Phe Ser Tyr

420 425 430

Gln Phe Leu Cys

435

<210> 19

<211> 866

<212> PRT

<213> 宏基因组(metagenomic)

<400> 19

Met Ala Val Asp Tyr Ser Leu Lys Asn Glu Trp Tyr Arg Glu Ile Asn

1 5 10 15

Lys Ser Cys Phe Thr Val Ala Leu Asn Val Ala Tyr Asp Asn Cys Lys

20 25 30

Ala Lys Gly His Glu Asn Leu Leu Arg Glu Ala Gln Arg Ser Lys Gly

35 40 45

Gly Ile Thr Asn Glu Gln Ile Lys Asn Val Gln Thr Glu Ile Lys Thr

50 55 60

Arg Leu Glu Asp Ile Arg Ser His Phe Ser His Phe Tyr His Asp Glu

65 70 75 80

Lys Ser Leu Ile Phe Glu Lys Asp Asn Ile Val Lys Asp Phe Leu Glu

85 90 95

Ser Ala Tyr Glu Lys Ala Gln Ser Ser Val Ile Gly Ser Thr Arg Gln

100 105 110

Ser Asp Tyr Lys Gly Val Val Pro Pro Leu Phe Glu Pro His Asp Gly

115 120 125

Met Ile Thr Ala Ala Gly Val Val Phe Leu Ala Ser Phe Phe Cys His

130 135 140

Arg Ser Asn Val Tyr Arg Met Leu Gly Ala Val Lys Gly Phe Lys His

145 150 155 160

Thr Gly Lys Glu Glu Leu Ser Asp Gly Ala Lys Arg Asp Tyr Gly Phe

165 170 175

Thr Arg Arg Leu Met Ala His Tyr Ser Leu Arg Asp Ser Tyr Val Ile

180 185 190

Lys Ala Glu Glu Thr Lys Ser Phe Arg Asp Leu Leu Gly Tyr Leu Ser

195 200 205

Arg Val Pro Gln Gln Ala Val Asp Trp Leu Asn Glu His Asn Gln Leu

210 215 220

Ser Glu Asp Glu Lys Lys Glu Phe Leu Asn Gln Lys Pro Ser Asp Glu

225 230 235 240

Glu Ser Gln Glu Gln Ser Lys Thr Glu Asn Thr Asp Arg Gln Ala Asp

245 250 255

Arg Met Pro Arg Arg Ser Leu Arg Lys Thr Asp Lys Phe Ile Leu Phe

260 265 270

Ala Ala Lys Phe Ile Glu Asp Trp Ala Gln Lys Glu Lys Met Asp Val

275 280 285

Thr Phe Ala Arg Tyr Gln Lys Thr Val Thr Glu Asp Glu Asn Lys Asn

290 295 300

Gln Asp Gly Lys Gln Val Arg Asp Val Gln Leu Lys Tyr Glu Lys Asp

305 310 315 320

Thr Lys Lys Leu Asn Pro Asp Phe Asp Tyr Lys Trp Thr Tyr Tyr Ile

325 330 335

Arg Asn Asn His Ala Ile Ile Gln Ile Lys Pro Asp Glu Tyr Lys Gln

340 345 350

Ala Val Ser Ala Arg Ile Ser Glu Asn Glu Leu Lys Tyr Leu Val Leu

355 360 365

Leu Ile Phe Gln Gly Lys Gly Trp Glu Ala Ile Lys Lys Ile Gly Asp

370 375 380

Tyr Ile Phe His Ile Gly Asn Lys Ile Lys Ile Gly Arg Phe Asp His

385 390 395 400

Asn Glu Glu Arg Arg Met Pro Ser Phe Leu Lys Asn Pro Pro Ala Asp

405 410 415

Ile Ile Gly Glu Met Val Glu Asn Arg Leu Lys Tyr Ile Arg Asp Glu

420 425 430

Leu Asn Lys Val Ile Glu Thr Ile Lys Lys Glu Glu Pro Gln Asn Asn

435 440 445

Lys Trp Leu Leu Tyr Lys Gly Lys Lys Ile Ser Ile Ile Leu Lys Phe

450 455 460

Ile Ser Asp Ser Ile Ser Asp Ile Lys Lys Arg Pro Asp Val Asn Glu

465 470 475 480

Tyr Asn Thr Leu Arg Asp Met Leu Gln Lys Leu Asp Phe Asp Asn Phe

485 490 495

Tyr Glu Arg Leu Lys Ser Tyr Val Ser Glu Gly Arg Ile Glu Gln Thr

500 505 510

Leu Tyr Asp Glu Ile Lys Gly Ile Lys Asp Ile Ser Thr Leu Cys Ile

515 520 525

Lys Ile Cys Glu Leu Arg Leu Ala Ala Leu Glu Glu Leu Glu Lys Glu

530 535 540

Gly Gly Asp Asp Leu Asn Lys Tyr Ile Gly Leu Ala Val Gln Glu Lys

545 550 555 560

His Lys Asn Tyr Asp Asp Ser Asn Thr Pro Gln Lys Lys Ala Glu Arg

565 570 575

Phe Leu Glu Ser Gln Phe Ser Val Gly Lys Asn Phe Leu Arg Glu Thr

580 585 590

Phe Tyr Asp Glu Tyr Ile Lys Asn Arg Lys Ser Leu Tyr Glu Ile Ile

595 600 605

Lys Glu Lys Ile Thr Gly Ile Thr Pro Leu Asn Glu Asn Arg Trp Tyr

610 615 620

Leu Met Asp Lys Asn Pro Lys Glu Phe Glu Ser Lys Asp Ser Lys Ile

625 630 635 640

Ile Arg Gly Leu Cys Asn Ile Tyr Ile Gln Asp Ile Leu Cys Met Lys

645 650 655

Ile Ala Leu Trp Tyr Tyr Glu Asn Leu Ser Pro Ser Tyr Lys Asn Lys

660 665 670

Leu Lys Trp Asp Phe Ile Gly Gln Gly Phe Gly Tyr Asp Arg Tyr Lys

675 680 685

Leu Ser Tyr Lys Thr Asp Cys Gly Ile Thr Ile Glu Phe Lys Leu Ala

690 695 700

Asp Leu Asn Arg Leu Asp Ile Ile Glu Lys Pro Lys Met Ile Glu Asn

705 710 715 720

Ile Cys His Ser Phe Ile Leu Glu Lys Asp Val Lys Lys Gln Thr Ile

725 730 735

Ser Trp His Glu Phe Arg Gln Asp Gly Ile Ala Lys Tyr Arg Lys Leu

740 745 750

Gln Lys Glu Val Val Glu Ala Val Phe Glu Phe Glu Asn Ser Leu Lys

755 760 765

Ile Pro Asp Lys Asn Trp Leu Thr Gln Gly Tyr Val Pro Phe Asn Lys

770 775 780

Asn Lys Arg Phe Glu Asp Lys Gly Phe Ser Thr Phe Ile Leu Glu Glu

785 790 795 800

Ala Val Arg Lys Gly Lys Ile Lys Ser Asp Asp Lys Glu Pro Leu Arg

805 810 815

Lys Val Arg Thr Asp Phe Phe His Glu Gln Phe Asp Ser Thr Asp Ala

820 825 830

Glu Arg Arg Ile Phe Asp Lys Tyr Met Pro Ala Lys His Asp Gly Lys

835 840 845

Asn Lys Gly Gly Lys Met Gln Glu Lys Gln Glu Lys Ser Tyr Thr Arg

850 855 860

Arg Ile

865

<210> 20

<211> 791

<212> PRT

<213> 宏基因组(metagenomic)

<400> 20

Met Asp Thr Ile Glu Leu Lys Lys Glu Glu Ala Ala Phe Tyr Phe Asn

1 5 10 15

Gln Ala Gly Phe Asn Leu Arg Ala Ile Glu Ser Asn Val Phe Asp Ala

20 25 30

Gly Lys Arg Lys Thr Leu Leu Glu Asn Pro Gln Val Leu Ala Lys Leu

35 40 45

Glu Asn Phe Ile Phe Asn Phe Arg Asp Val Thr Lys Asn Glu Lys Gly

50 55 60

Glu Ile Asp Val Leu Ile Ser Lys Leu Thr Asp Leu Arg Asn Tyr Tyr

65 70 75 80

Ser His Tyr Val His Thr Asp Asn Val Lys Val Leu Ser Lys Gly Glu

85 90 95

Gly Pro Ile Leu Ala Arg Tyr Tyr Gln Phe Ala Thr Glu Ala Thr Ala

100 105 110

Ser Thr Asn Val Lys Leu Glu Ile Met Asp Lys Gly Asn Lys Leu Thr

115 120 125

Asp Ala Gly Val Leu Phe Phe Leu Cys Met Phe Leu Lys Lys Ser Gln

130 135 140

Ala Asn Lys Leu Ile Ser Ser Ile Ser Gly Phe Lys Arg Thr Asp Ala

145 150 155 160

Glu Gly Gln Pro Arg Arg Asn Leu Phe Thr Tyr Phe Ser Val Arg Glu

165 170 175

Gly Tyr Lys Val Val Pro Glu Met Gln Lys His Phe Leu Leu Phe Asp

180 185 190

Leu Val Asn His Leu Ser Asn Gln Asp Glu Tyr Ile Glu Lys Ser Gln

195 200 205

Gln Thr Tyr Asp Ile Gly Glu Gly Leu Phe Phe His Arg Met Ala Ser

210 215 220

Lys Phe Leu Asn Thr Ser Gly Ile Leu Arg Gly Met Lys Phe Tyr Ala

225 230 235 240

Tyr Gln Ser Lys Arg Leu Glu Glu Lys Arg Gly Lys Ile Glu Pro Glu

245 250 255

Gly Asp Ser Phe Val Trp Ile Glu Pro Phe Gln Gly Asn Ser Tyr Phe

260 265 270

Glu Val Asp Gly His Lys Gly Val Ile Gly Glu Asp Glu Leu Lys Asp

275 280 285

Leu Cys Tyr Ala Leu Leu Val Ala Gly Lys Asn Ala Asn Glu Ala Glu

290 295 300

Gly Lys Ile Thr Gln Phe Leu Thr Lys Tyr Lys Lys Ala Asp Asn Ser

305 310 315 320

Gln Glu Ile Glu Lys Asp Glu Met Leu Cys Ile Asp Asn Phe Pro Ala

325 330 335

Asn Tyr Phe Asp Gly Pro Gly Val Gly Ser Ile Lys Asp Arg Val Leu

340 345 350

Asn Arg Leu Glu Arg Glu Ile Lys Ser His Lys Asp Asn Lys Ala Asp

355 360 365

Ser Lys Ala Tyr Asp Lys Met Lys Glu Val Met Asp Phe Ile Asn Asn

370 375 380

Arg Leu Pro Ala Ala Glu Lys Met Lys Gln Lys Asp Tyr Arg Arg Tyr

385 390 395 400

Leu Lys Met Val Arg Leu Trp Asn Arg Glu Lys Gly Asn Ile Glu Arg

405 410 415

Glu Phe Lys Ala Lys Glu Trp Ser Lys Tyr Phe Pro Ser Asn Phe Trp

420 425 430

Arg Ala Asn Asn Leu Glu Asp Val Tyr Lys Leu Ala Arg Gln Glu Asn

435 440 445

Ala Arg Ile Leu Gly Asn Leu Lys Ala Val Val Glu Gly Met Ser Glu

450 455 460

Gln Glu Phe Glu Lys Tyr Arg Gln Ile Asn Glu Ala Lys Asp Leu Ala

465 470 475 480

Gly Leu Arg Gln Leu Ala Gly Ser Phe Gly Val Lys Trp Glu Glu Lys

485 490 495

Asp Trp Glu Glu Tyr Ala Arg Gln Ile Lys Glu Arg Ile Thr Asp Arg

500 505 510

Gln Lys Leu Thr Ile Met Lys Gln Arg Ile Thr Ala Glu Leu Lys Lys

515 520 525

Arg His Ala Ile Glu Asn Leu Asn Leu Arg Ile Thr Ile Asp Ser Ser

530 535 540

Lys Ser Arg Ala Ala Val Leu Asn Arg Ile Ala Leu Pro Lys Gly Phe

545 550 555 560

Val Lys Thr His Ile Leu Gln Thr Pro Ser Asp Lys Ile Met Lys Lys

565 570 575

Thr Arg Glu Ala Glu Cys Lys Ile Leu Leu Ser Gly Lys Tyr Gly Asp

580 585 590

Leu Ser Lys Arg Phe Phe Asp Glu Lys Asn Leu Asp Lys Leu Thr Gln

595 600 605

Ile Asn Gly Leu Tyr Glu Lys Asn Lys Ile Ile Ala Phe Met Val Val

610 615 620

Tyr Leu Met Glu Gln Leu Lys Leu Gly Leu Lys Gly Lys Thr Lys Leu

625 630 635 640

Ala Glu Leu Lys Glu Thr Arg Ile Arg Tyr Lys Ile Ser Asp Lys Val

645 650 655

Thr Glu Asp Ile Gln Leu Ser Gln Tyr Pro Ser Leu Val Tyr Val Ile

660 665 670

Gly Arg Lys Tyr Ile Asp Asn Val Asp Arg Tyr Lys Phe Ala Gly Asp

675 680 685

Val Arg Gly Lys Pro Ile Leu Ser Lys Ile Asp Leu Ile Glu Lys Glu

690 695 700

Arg Leu Glu Phe Ile Arg Gln Val Leu Gly Phe Glu Lys Glu Leu Phe

705 710 715 720

Asp Asn Thr Val Ile Asp Lys Lys Asn Phe Thr Asp Thr Glu Thr His

725 730 735

Ile Lys Phe Arg Gln Ile Ile Asp Glu Leu Val Arg Lys Gly Trp Asp

740 745 750

Ala Thr Lys Leu Asn Lys Leu Lys Glu Ala Arg Asn Ala Ala Leu His

755 760 765

Gly Glu Ile Pro Glu Gly Thr Ser Phe Gln Glu Ala Asn Val Leu Ile

770 775 780

Asn Glu Leu Lys Asn Lys Lys

785 790

<210> 21

<211> 767

<212> PRT

<213> 宏基因组(metagenomic)

<400> 21

Leu Tyr Gly Phe Asp Lys Leu Gln Lys Phe Asn Glu Lys Asn Gln Pro

1 5 10 15

Glu Glu Trp Lys Asn Leu Ile Lys Asp Leu Asp Tyr Phe Glu Ser Ser

20 25 30

Asp Leu Pro Phe Val Ser Lys Thr Thr Pro His Tyr His Phe Ile Ala

35 40 45

Asn Lys Ile Gly Ile Lys Phe Val Tyr Arg Asn Asn Lys Lys Trp Pro

50 55 60

Ser Leu Glu Val Lys Glu Lys Ser Glu Gly Lys Ile Lys Tyr Asn Tyr

65 70 75 80

Asp Lys Gln Phe Val Ala Asp Ala Phe Ile Ser Val His Glu Leu Val

85 90 95

Pro Met Met Phe Tyr Tyr Leu Leu Thr Lys Asn Gly Asn Ser Ile Glu

100 105 110

Lys Ile Ile Lys Gln Thr Ile Asp Asp Val Tyr Asn Ile Tyr Asp Glu

115 120 125

Phe Lys Asp Asn Lys Ile Asn Asp Leu Asp Asp Leu Val Gln Lys Ile

130 135 140

Ser Lys Ser Asn Ile Leu Ile Gly His Leu Pro Lys Gln Met Ile Ser

145 150 155 160

Ile Leu Lys Lys Thr Gln Lys Asn Met Gln Asn Glu Ala Ser Ile Lys

165 170 175

Leu Ile Lys Leu Ile Glu Asp Thr Glu Lys Arg Lys Asn Lys Leu Glu

180 185 190

Asp Gln Ile Lys Gln Lys Ile Arg Ile Gly Lys Arg Asn Ser Gly Leu

195 200 205

Leu Lys Ser Gly Ile Ile Ala Asp Trp Leu Ile Lys Asp Leu Met Arg

210 215 220

Phe Gln Pro Val Ala Leu Asp Ser Asn Lys Asn Pro Leu Asn Arg Ser

225 230 235 240

Lys Ala Asn Ser Thr Glu Tyr Gln Leu Leu Gln Lys Thr Phe Ala Leu

245 250 255

Tyr Gly Gly Glu Lys Asp Arg Leu Pro Ala Tyr Phe Lys Gln Leu Asn

260 265 270

Leu Ile His Ser Asp Asn Pro His Pro Phe Leu Asn Ala Phe Asp Trp

275 280 285

Asp Asn Gln Pro Asn Ile Leu Ser Phe Tyr Glu Lys Tyr Leu Asn Ala

290 295 300

Lys Leu Asp Tyr Leu Lys Gly Leu Ser Pro Lys Asn Trp Gln Lys His

305 310 315 320

Gln His Phe Leu Leu Leu Lys Val Pro Lys Thr Asp Arg Asn Thr Leu

325 330 335

Val Ala Gly Trp Lys Asn Tyr Phe Asn Leu Pro Arg Gly Leu Phe Thr

340 345 350

Gln Ala Ile Lys Asp Trp Ile Ile Glu His Glu Glu Ser Pro Leu Lys

355 360 365

Lys Lys Ile Asn Ile Phe Asp Arg Pro Gly Phe Ile Val Lys Thr Ile

370 375 380

Pro Leu Tyr Phe Arg Glu Lys Asn Asn Asp Asp Val Pro Lys His Tyr

385 390 395 400

Gln Tyr Asp Cys Asn Val Ala Asn Pro Leu Lys Leu Lys Ala Ala Asn

405 410 415

Phe Ile Ser Ile Glu Asn Arg Asn Glu Tyr Leu Ile Lys Asn Lys Lys

420 425 430

Val Leu Asn Leu Gln Lys Thr Asn Asp Gln Ile Ile Lys Ile Asn Ser

435 440 445

Val Phe Asp Ser Val Glu Asn Asn Asp Arg Ser Trp Val Glu Lys Ile

450 455 460

Val Glu Leu Lys Lys Leu Met His Glu Leu Glu Leu Pro Lys Lys Leu

465 470 475 480

Lys Asp Lys Leu Glu Asn Arg Ile Asn Leu Lys Ser Leu Lys Glu Glu

485 490 495

Val Ile Ser Ala Ala Lys Lys Pro Tyr Leu Asp Phe Lys Lys Trp Arg

500 505 510

Lys Phe Glu Lys Glu Met Arg Leu Phe Lys Asn Gln Asp Ile Leu Met

515 520 525

Trp Leu Met Cys Lys Asp Leu Gln Thr Asp Lys Gln Met Thr Ser Leu

530 535 540

Ala Ile Asn Asp Leu Tyr Leu Lys Asn Ile Asn Thr Asp Val His Ser

545 550 555 560

Val Gly Ser Leu Asn Ile Leu Asn Arg Val Leu Pro Met Lys Leu Ser

565 570 575

Ile Asp Val Phe Pro Thr Asp Asp Lys Gly Asn Val Leu Arg Asn Lys

580 585 590

Asp Ser Ile Asn Lys Val Tyr Ile Glu Glu Lys Gln Thr Lys Val Leu

595 600 605

Lys Gln Gly Asn Phe Lys Ala Leu Met Lys Asp Arg Arg Leu Asn Cys

610 615 620

Leu Phe Ser Phe Ile Glu Ser Glu Gly Val Asp Met Glu Asn Asn Pro

625 630 635 640

Ile Ser Lys Leu Arg Leu Glu Tyr Glu Leu Ala Lys Tyr Gln Thr Ser

645 650 655

Arg Ile Asn Ile Phe Glu Ile Thr Leu Asp Ile Glu Lys Asn Leu Ile

660 665 670

Ser Lys His Pro Asn Leu Pro Ser Tyr Asn Phe Arg Glu Met Ile Glu

675 680 685

Val Trp Ile Lys Gln Lys Asp Asn Arg Asn Glu Leu Glu His Phe Thr

690 695 700

Lys Pro Leu Ile Ala Ile Arg Asn Ala Phe Ser His Asn Gln Tyr Pro

705 710 715 720

Met Tyr Asn Lys Thr Leu Phe Lys Asp Ile Arg Leu Phe Asn Ile Ile

725 730 735

Glu Pro Asn Ile Asp Glu Ser Gln Gly Leu Asn Ile Ala Phe Gln Leu

740 745 750

Thr Glu Leu Thr Lys Glu Leu Lys Thr Ile Ile Asn Lys Asn Ile

755 760 765

<210> 22

<211> 598

<212> PRT

<213> 宏基因组(metagenomic)

<400> 22

Met Gly Phe Ser Ile Lys Lys Leu Arg Glu Ile Ile Leu Glu Gly Thr

1 5 10 15

Glu Tyr Asn Asp Asn Lys Tyr Asp Thr Val Arg Asn Lys Leu Tyr Gln

20 25 30

Ile Val Asp Phe Ile Leu Tyr Arg Gly Tyr Ile Asn Glu Asn Ser Glu

35 40 45

Arg Ala Glu Val Leu Val Asn Ala Leu Arg Ser Thr Leu Asn Glu Asp

50 55 60

Asp Lys Thr Lys Leu Tyr Ser Ser Glu Ala Ala Phe Leu Lys Lys Lys

65 70 75 80

Tyr Met Lys Ile Ile Arg Lys Ala Ala Asp Ser Leu Asp Val Lys Lys

85 90 95

Leu Lys Asp Leu Lys Lys Lys Ala Phe Thr Ile Pro Asp Asn Glu Leu

100 105 110

Arg Lys Cys Phe Ile Ser Tyr Ala Asp Ser Val Ser Glu Phe Thr Lys

115 120 125

Leu Ile Tyr Leu Leu Thr Arg Phe Leu Ser Gly Lys Glu Ile Asn Asp

130 135 140

Leu Val Thr Thr Leu Ile Asn Lys Phe Asp Asn Ile Arg Ser Phe Leu

145 150 155 160

Glu Ile Met Asp Glu Leu Gly Leu Glu Arg Thr Phe Thr Asp Glu Tyr

165 170 175

Arg Phe Phe Glu Gly Ser Thr Lys Tyr Leu Ala Glu Leu Val Glu Leu

180 185 190

Asn Ser Phe Val Lys Ser Cys Ser Phe Asp Met Ser Ala Lys Arg Thr

195 200 205

Met Tyr Arg Asp Ala Leu Asp Ile Leu Gly Ile Glu Ser Asp Lys Thr

210 215 220

Glu Glu Asp Ile Glu Lys Met Ile Asp Asn Ile Leu Gln Val Asp Ala

225 230 235 240

Asn Gly Lys Lys Leu Pro Asn Lys Asn His Gly Leu Arg Asn Phe Ile

245 250 255

Ala Ser Asn Val Ile Asp Ser Asn Arg Phe Glu Tyr Leu Val Arg Tyr

260 265 270

Gly Asn Pro Lys Lys Ile Arg Glu Thr Ala Lys Cys Glu Pro Ala Val

275 280 285

Arg Phe Val Leu Asn Glu Ile Pro Asp Ala Gln Ile Glu Arg Tyr Tyr

290 295 300

Lys Ala Tyr Tyr Pro Asp Glu Lys Ser Leu Cys Leu Ala Asn Met Gln

305 310 315 320

Arg Asp Lys Leu Ala Gly Val Ile Ala Asp Ile Lys Phe Asp Asp Phe

325 330 335

Ser Asp Ala Gly Ser Tyr Gln Lys Ala Asn Ala Thr Ser Thr Arg Arg

340 345 350

Thr Ser Glu Ala Glu Ile Lys Arg Lys Asn Gln Ala Ile Ile Arg Leu

355 360 365

Tyr Leu Thr Val Met Tyr Ile Met Leu Lys Asn Leu Val Asn Val Asn

370 375 380

Ala Arg Tyr Val Ile Ala Phe His Cys Leu Glu Arg Asp Ala Lys Leu

385 390 395 400

Tyr Ser Glu Ser Val Leu Lys Val Gly Asn Thr Asn Glu Glu Ser Arg

405 410 415

Leu Gln Thr Gly Asn Thr Asn Glu Glu Lys Asn Lys Val Lys Leu Thr

420 425 430

Asn Leu Thr Asn Leu Thr Met Ala Val Met Gly Val Lys Leu Glu Asn

435 440 445

Gly Thr Ile Lys Thr Glu Phe Asp Lys Ser Leu Ala Glu Asn Ala Ala

450 455 460

Asn Arg Tyr Leu Arg Asn Ala Arg Trp Tyr Lys Leu Ile Leu Asp Asn

465 470 475 480

Leu Lys Lys Ser Glu Arg Ala Val Val Thr Glu Phe Arg Asn Thr Val

485 490 495

Cys His Leu Asn Ala Ile Arg Asn Ile Asn Ile Asn Ile Lys Glu Val

500 505 510

Lys Glu Val Glu Asn Tyr Phe Ala Leu Tyr His Tyr Leu Ile Gln Lys

515 520 525

His Leu Glu Asn Arg Phe Ala Asp Lys Lys Val Glu Arg Asp Thr Gly

530 535 540

Asp Phe Ile Ser Lys Leu Glu Glu His Lys Thr Tyr Cys Lys Asp Phe

545 550 555 560

Val Lys Ala Tyr Cys Thr Pro Phe Gly Tyr Asn Leu Val Arg Tyr Lys

565 570 575

Asn Leu Thr Ile Asp Gly Leu Phe Asp Lys Asn Tyr Pro Gly Lys Asp

580 585 590

Asp Ser Asp Lys Gln Lys

595

<210> 23

<211> 699

<212> PRT

<213> 宏基因组(metagenomic)

<400> 23

Met Asn His Gln Thr Arg Lys Ser Thr Ser Lys Arg Leu Gly Val Gln

1 5 10 15

Thr Ile Val Asn Lys Asn Asn Asn Ile Phe Val Phe Ser Asn Asn Gln

20 25 30

Pro Lys Thr Lys Ser Val Gln Leu Ala Asp Glu His Gln Gln Leu Ile

35 40 45

Glu Gln Asn Asn Asp Leu Asp Asn Phe Lys Thr Asp Leu Glu Tyr Gly

50 55 60

Asn Ile Lys Phe Gly Leu Ser Asn Asn Ala Leu Glu Ala Phe Tyr Pro

65 70 75 80

Asn Gln Tyr Thr Ile Arg Lys Lys Ser Ser Ser Ile Asn Gln Trp Leu

85 90 95

Val Gln Lys Tyr Cys Pro Thr Gln Cys Pro Asp Ser Asn Leu Ile Leu

100 105 110

Gln Leu Ile His Asn Asn Glu Asp Phe Lys Lys Ile Ile Ala Leu Tyr

115 120 125

Ser Asp Val Val Phe Ser Met Lys Val Phe Lys Gly Asn Arg Asn Glu

130 135 140

Asp Tyr Ile Ser Lys Ile Phe Asn Asn Met Asn Ala Lys Glu Ile Ile

145 150 155 160

Asp Lys Val Lys Ser Val Ile Asp Ser Ile Lys Lys Arg Pro Tyr Leu

165 170 175

Phe Gly Phe Asn Pro Lys Asp Ser Ala Ser Asp Gln Gln Ile Ala Tyr

180 185 190

His Leu Lys Asn Ile Ser Met Leu Arg Asn Tyr Ile Thr His Asp Ser

195 200 205

Asn Asp Asn Leu Arg Ile Asp Ser Asp Thr Tyr Ala Ser Leu Thr Glu

210 215 220

Gln Leu Asp Gln Phe Ile Glu Gln Glu Asn Lys Lys Phe Thr Glu Gln

225 230 235 240

Asn Gly His Asn Leu Ala Leu Ile Ser Gln Phe Tyr Pro Ser Trp Ser

245 250 255

Asp Glu Glu Val Val Lys Asn Tyr His Asp Tyr Tyr Phe Asn Met Leu

260 265 270

Asp Lys Asn Leu Gly Val Asn Leu Arg Gln Ile Ala Leu Leu Ile Arg

275 280 285

Glu Lys Asn Asn Ile Ser Gln Met Leu Asp Asp Asp Arg Arg Ala Lys

290 295 300

Phe Asn Val Val Leu Lys Phe Tyr Leu Tyr Thr Trp Leu Lys Ala Asn

305 310 315 320

Gln Ala Gln Leu Asp Asp Tyr Ile Gln Gln Leu Arg Gln Ala Gln Leu

325 330 335

Asp Asp Lys Asp Thr Ile Tyr Gln Lys Ile Ala Gln Asp Ile Pro Ala

340 345 350

Asn Asp Ile Asn Arg Leu Ile Lys Leu Cys Gln Gln Ile Asp Phe Gln

355 360 365

Ile Lys Pro Thr Arg Phe Lys Arg Ile Pro Ile Lys Thr Ile Asn Glu

370 375 380

Gln Trp Asp Phe Arg Val Leu Met Ile Tyr Ser Leu Thr Lys Phe Leu

385 390 395 400

Thr Ile Lys Glu Thr Asn Glu Leu Ile Ser Ala Leu Leu Asn Lys Ile

405 410 415

Glu Ala Met Ser Asn Leu Leu Glu Ile Asp Arg Ala Leu Lys Val Gln

420 425 430

Ala Val Asp Ala Ile Gln Pro Asn Gln Trp Phe Phe Val Ser Leu Pro

435 440 445

Met Pro Lys Lys Pro Val Lys Asn Thr Lys Gln Pro Lys Pro Lys His

450 455 460

Gln Ser Gln Ser Lys Pro Ala Asn Gly Ile Arg Gly Leu Ala Glu Leu

465 470 475 480

Arg Ala Gln Phe Gln Pro Ser Ile Thr His Ser Val Ser Ser Asp Asn

485 490 495

Gln Leu Ile Gln His Gln Pro Val Ile Thr Ser Leu Lys Ser Ile Asp

500 505 510

Tyr Gln Arg Ile Thr Arg Gln Leu Thr Ile Val Asn Arg Ile Arg Ser

515 520 525

Tyr His Gln Pro Lys His Ala Glu Tyr His Arg Gln Glu Leu Ile Asp

530 535 540

Ala Ile Ser Leu Phe Trp Gly Ser Asp Glu Leu Cys Ile Thr Asp Gly

545 550 555 560

Ile Phe Asp Glu Glu Ile Lys Val Leu Val Asn Gly Ser Thr Lys Pro

565 570 575

Val Ser Arg Lys Leu Lys Asn Ile Leu Arg Asn Asn Val Ile Lys Thr

580 585 590

Lys Phe Phe Asn Tyr Leu Val Arg Tyr Ile Glu Pro Lys Lys Val Ala

595 600 605

Asn Leu Leu His Asn Asn Tyr Gly Leu Ala Leu Phe Leu Val Gln Gln

610 615 620

Leu Thr Pro Ser Gln Leu Glu Ser Leu Met Ile Glu Tyr Ile Pro Leu

625 630 635 640

Glu Asp Glu Asn Gln Leu Val Gly Gln Thr Pro Arg Glu Glu Leu Thr

645 650 655

Glu Ala Gln Lys Gln Arg Leu Glu Gln Tyr Glu His Gln Leu Ala Met

660 665 670

Ile Phe Ser Glu Lys Ile Ser Leu Thr Tyr Cys Val Asn Arg Lys Met

675 680 685

Thr Val Gly Arg Leu Ala Leu Leu Asn His Ile

690 695

<210> 24

<211> 687

<212> PRT

<213> 宏基因组(metagenomic)

<400> 24

Met Glu Ser Glu Glu Phe Phe Lys Tyr Leu Lys Glu Gly Lys Tyr Glu

1 5 10 15

Lys Leu Ala Lys Leu Ile Asn Lys Asp Asp Thr Ala Leu Thr Phe Leu

20 25 30

Asp Glu Ala Leu Ser Ser Glu Val Lys Asp Leu Val Arg Arg Ser Phe

35 40 45

Leu Ile Leu Lys Arg Leu Asp Asp Glu Tyr Ile Val Arg Tyr Leu Pro

50 55 60

Tyr Ile Ile Leu Tyr Leu Lys Glu Lys Arg Ala Ile Ser Lys Glu Ala

65 70 75 80

Glu Glu Ile Leu Lys Asn Phe Thr Asn Ser Glu Asn Ile Asp Asp Ala

85 90 95

Ile Ile Asn Leu Leu Lys Met Pro Ile Asn Asp Ser Ile Leu Glu Glu

100 105 110

Leu Ile Tyr Leu Tyr Leu Glu Asn Ala Asn Ser Glu Asn Val Phe Tyr

115 120 125

Lys Ala Ile Lys Glu Tyr Ser Ala Ile Glu Glu Leu Met Lys Lys Tyr

130 135 140

Asp Ser Lys Arg Ile Leu Glu Ile Leu Thr His Lys Leu Tyr Ser Asp

145 150 155 160

Glu Lys Leu Glu Arg Asp Phe Ala Leu Asn Val Ile Tyr Asp Ile Val

165 170 175

Glu Leu Leu Ser Glu Asp Glu Lys Ala Ile Leu Lys Lys His Leu Asp

180 185 190

Ile Ser Ile Leu Gly Tyr Phe Asn Lys Arg Ser Tyr Lys Lys Ile Lys

195 200 205

His Leu Phe Glu Ile Leu Ser Ile Lys Pro Glu Ile Ser Glu Glu Asp

210 215 220

Ile Lys Lys Ile Leu Lys Thr Asp Gly Lys Ala Gly Leu Asn Ile Ile

225 230 235 240

Leu Arg Glu Asn Ile Ser Leu Ser Ser Lys Phe Tyr Asp Arg Glu Phe

245 250 255

Leu Arg Glu Tyr Leu Tyr Ser Gly Asn Glu Glu Lys Gln Phe Ile Gly

260 265 270

Val Lys Leu Ile Ser Leu Arg Ser Asp Ser Lys Ser Lys Ile Asp Leu

275 280 285

Leu Phe Lys Phe Leu Asn Tyr Gly Tyr Gly Lys Ala Lys Thr Ser Ala

290 295 300

Ile Arg Glu Leu Lys Lys Ile Ala Lys Asn Asn Ala Glu Leu Lys Ser

305 310 315 320

Tyr Ile Glu Asn Lys Thr Leu Met Tyr Ala Lys Lys Met Asn Leu Ser

325 330 335

Leu Lys Ile Ser Ser Leu Arg Ile Leu Lys Glu Ile Ala Lys Lys Glu

340 345 350

His Leu Asn Phe Leu Ile Asn Glu His Lys Arg Leu Lys Asn Leu Ala

355 360 365

Tyr Arg Leu Glu Glu Glu Lys Phe Met Gly Gly Phe Arg His Met Ile

370 375 380

Met Met Glu Glu Glu Ile Arg Lys Cys Asn Val Ala Met Asn Leu Ile

385 390 395 400

Glu Glu Thr Val Ala Arg Ile Cys Leu Asp Asn Asn Ile His Tyr Lys

405 410 415

Thr Leu Lys Ile Ala Glu Asn Leu Gly Tyr Glu Phe Tyr Lys Thr Ile

420 425 430

Glu Ile Ile Gly Ser Lys Asn Leu Asp Leu Ile Asn Ile Asn Glu Leu

435 440 445

Leu Asp Asp Ile Lys Arg Asp Gly Glu Leu Ile Ile Tyr Leu Thr Asn

450 455 460

Ile Ile Ala Asn Asn Arg Asn Ile Asp Glu His Leu Lys Asn Glu Ile

465 470 475 480

Ile Lys Val Ile Glu Asn Ile Asp Met Ala Asp Arg Asp Ile Leu Asn

485 490 495

Ala Asn Lys Ile Ile Ile Tyr Ser Ser Phe Asn Met Val Glu Lys Ile

500 505 510

Gly Glu Ile Ile Asn Met Ala Glu Gly Tyr Tyr Ser Lys Leu Ala Phe

515 520 525

Ile Asn Ala Val Lys Lys Phe Ile Glu Ser Gly Gln Phe Lys Glu Asp

530 535 540

His Leu Cys Tyr Val Val Pro Lys Ile Ala Glu Met Leu Tyr Ser Thr

545 550 555 560

Lys Lys Leu Arg Leu Met Ala Leu Glu Phe Phe Lys Glu Tyr Pro Asn

565 570 575

Glu Ile Ala Leu Pro Ile Leu Leu Lys Glu Leu Gly Asn His Arg Arg

580 585 590

Glu Glu Lys Leu Thr Ile Glu Val Ile Ser Asn Ile Ile Glu Lys Phe

595 600 605

Pro Tyr Ser Val Ile Lys Ile Lys Asn Leu Leu Asn Thr Glu Lys Arg

610 615 620

Glu Leu Ala Leu Lys Ile Leu Phe Lys Ile Ser Lys Asn Asn Pro Glu

625 630 635 640

Ile Leu Lys Asp Phe Thr Tyr Leu Leu Ala Gly Ile Tyr Glu Asn Ala

645 650 655

Ser Arg Glu Glu Lys Ser Leu Ile Gly Lys Ile Leu Arg Asn Ile Ile

660 665 670

Asn Glu Glu Gln Lys Leu Ile Leu Lys Pro Ile Ile Gly Asp Ile

675 680 685

<210> 25

<211> 768

<212> PRT

<213> 宏基因组(metagenomic)

<400> 25

Leu Asn Glu Ile Arg Gln Trp Thr Ala His Tyr Glu Ser Ala Tyr Lys

1 5 10 15

Ile Phe Ser Gly Ser Glu Leu Ile Lys Glu Glu Leu Gly Ala Asn Lys

20 25 30

Glu Pro Gln Asn Asn Arg Trp Met Val Ile Glu Ser Tyr Tyr Lys Gly

35 40 45

Leu Val Asn Arg Ile Asn Arg Gly Phe Leu Asn Asn Ser Ala Leu Tyr

50 55 60

Asn Phe Pro Leu Leu Phe Asp Leu Val Lys Pro Lys Asp Glu Asn Glu

65 70 75 80

Lys Lys Lys Leu Ile Glu Glu Tyr Tyr Lys Phe Ser Val Leu Lys Asp

85 90 95

Gly Lys Asn Val Gly Val Asn Leu Arg Thr Val Arg Glu Ala Met Phe

100 105 110

Lys Asn Gly Phe His Asp Val Lys Glu Lys Glu His Asp Thr Tyr Arg

115 120 125

Ser Lys Phe Tyr Gly Ile Val Asp Phe Leu Leu Tyr Arg Ser Glu Ser

130 135 140

Lys Asn Phe Ser Gly Trp Ser Glu Glu Leu Arg Lys Thr Val Asp Lys

145 150 155 160

Val Ala Lys Glu Lys Cys Tyr Asp Lys Trp Gly Glu Ser Val Ala Ala

165 170 175

Arg Met Arg Pro Lys Val Thr Ala Leu Leu Asp Lys Leu Ser Lys Met

180 185 190

Glu Trp Lys Lys Lys Pro Asn Lys Glu Asp Leu Glu Lys Leu Ala Glu

195 200 205

Pro Tyr Lys Ser Tyr Leu Ala Asp Val Gln Leu Ser Ala Asp Asp Ala

210 215 220

Asp Pro Leu Val Lys Leu Leu Ala Phe Leu Cys Asn Phe Trp Glu Gly

225 230 235 240

Lys Glu Ile Asn Glu Leu Leu Ser Ala Tyr Ile His Lys Phe Glu Asn

245 250 255

Ile Gln Glu Phe Ile Asp Thr Ile Glu Lys Leu Glu Gly Lys Lys Pro

260 265 270

Thr Phe Ser Glu Lys Tyr Ala Leu Phe Asn Glu Pro Ala Arg Arg Arg

275 280 285

Thr Glu Glu Val Ala Glu Ile Pro Gly Thr Arg Ala Gly Glu Ile Ala

290 295 300

Gln Asn Leu Arg Val Leu Ala Ser Ile Gly Lys Met Lys Pro Asp Leu

305 310 315 320

Gly Asp Ala Lys Arg Gln Leu Tyr Lys Ala Ala Ile Glu Met Leu Gly

325 330 335

Val Pro Asp Asp Ser Gln Phe Val Ser Asp Gln Trp Leu Ala Glu Asn

340 345 350

Val Leu Leu Asp Lys Thr Gln Ser Gly Tyr Ala Asn Arg Lys Thr Glu

355 360 365

Val Asn Pro Phe Arg Asn Phe Val Ala Lys Gln Val Ile Glu Ser Arg

370 375 380

Arg Phe Met Tyr Leu Val Arg Tyr Thr Lys Pro Lys Thr Val Arg Thr

385 390 395 400

Leu Met Glu Asn Lys Ala Val Val His Tyr Val Leu Ser Arg Ile Asp

405 410 415

Asn Ser Lys Asn Val Lys Gly Lys Glu Asp Ile Ile Thr Ser Tyr Tyr

420 425 430

Lys Thr Leu Lys Glu Tyr Asp Pro Gln Lys His Gln His Leu Pro Thr

435 440 445

Asp Lys Ala Glu Arg Glu Lys Glu Ile Asn Arg Arg Ile Asp Ala Leu

450 455 460

Ala Asp Tyr Leu Cys Lys Tyr Ser Phe Glu Lys Asn Val Leu Lys Gln

465 470 475 480

Lys Asp Gly Ile Val Arg Asn Thr Lys Ser Ala Thr Lys Asn Val Glu

485 490 495

Ile Glu Arg Leu Lys Ala Leu Thr Gly Leu Tyr Leu Thr Val Ala Tyr

500 505 510

Ile Ala Ile Lys Asn Leu Val Lys Ala Asn Ala Arg Tyr Tyr Ile Ala

515 520 525

Phe Ser Ile Phe Glu Arg Asp Tyr Ala Leu Phe Glu Lys Lys Leu Gly

530 535 540

Arg Asp Ala Leu Asp Glu Arg Pro Lys Tyr Pro Asp Asp Lys Gly Lys

545 550 555 560

Glu Thr Glu Ala Asp Tyr Asn Phe Phe Ala Leu Thr Lys Tyr Leu Leu

565 570 575

Asp Lys Asp Asp Lys Ala Phe Gly Ser Trp Lys Pro Tyr Gln Trp Asp

580 585 590

Glu Ser Lys Asn Lys Gly Glu Asn Trp Lys Ala Leu Arg Leu His Ile

595 600 605

Lys Gln His Lys Lys Glu Asn Gln Arg His Phe Ser Gln Lys Trp Leu

610 615 620

Asn Phe Phe Arg Gln Gln Ile Glu Asn Ala Lys Asn Ile Ser Glu Thr

625 630 635 640

Gly Tyr Leu Leu Thr Ala Ala Arg Asn His Ala Glu His Leu Asn Val

645 650 655

Leu Thr Ala Leu Pro Lys Phe Val Gly Glu Phe Arg Lys Thr Glu Gly

660 665 670

Lys Met Thr Ser Tyr Phe Glu Leu Tyr His Phe Leu Leu Gln Lys Leu

675 680 685

Met Leu Ala Asp Ala Gly Leu Lys Asn Leu Asp Lys Tyr Arg Glu Arg

690 695 700

Ile Asn Lys Tyr Gln Thr Ser Cys Lys Asp Leu Ile Lys Ile Thr Tyr

705 710 715 720

Val Ser Leu Gly Tyr Asn Leu Ala Arg Tyr Lys Asn Leu Thr Ile Glu

725 730 735

Ala Leu Phe Asp Ser Asp Ser Glu Asn Gly Lys Ala Leu Thr Lys Glu

740 745 750

Arg Glu Lys Lys Ala Glu Glu Arg Ala Lys Glu Arg Ala Lys Lys His

755 760 765

<210> 26

<211> 340

<212> RNA

<213> 宏基因组(metagenomic)

<400> 26

aauccaugac caaagaaucg ucacaaaucg aagcuuacaa aauggaguaa aauuuuuuuu 60

acucaguaau augcuuuggg uugaaagucu cccaccaauu cguaugcgga aaacguaaug 120

agauuuaaaa auuuuaaauu guuuaaauca acucauuaag gaggaugccc uuggguauuc 180

ugcuucuuga ccugguaccu cuauugcagg guacuggugu uuucuucggu acuggauucc 240

guuuguaugg aaucuaaacc auaguuauga cgauugcucu uucccgugcu ggaucgagua 300

acccaaugga gcuuacuauu cuugguccau ggauucaccc 340

<210> 27

<211> 699

<212> PRT

<213> 宏基因组(metagenomic)

<400> 27

Ala Ala Val Glu Asp Asn His Leu Leu Ile Lys Ala Val Gln Asn Glu

1 5 10 15

Asp Val Asp Leu Val Gln Gln Leu Leu Glu Gly Gly Ala Asn Val Asn

20 25 30

Phe Gln Glu Glu Glu Gly Gly Trp Thr Pro Leu His Asn Ala Val Gln

35 40 45

Met Ser Arg Glu Asp Ile Val Glu Leu Leu Leu Arg His Gly Ala Asp

50 55 60

Pro Val Leu Arg Lys Lys Asn Gly Ala Thr Pro Phe Ile Leu Ala Ala

65 70 75 80

Ile Ala Gly Ser Val Lys Leu Leu Lys Leu Phe Leu Ser Lys Gly Ala

85 90 95

Asp Val Asn Glu Cys Asp Phe Tyr Gly Phe Thr Ala Phe Met Glu Ala

100 105 110

Ala Val Tyr Gly Lys Val Lys Ala Leu Lys Phe Leu Tyr Lys Arg Gly

115 120 125

Ala Asn Val Asn Leu Arg Arg Lys Thr Lys Glu Asp Gln Glu Arg Leu

130 135 140

Arg Lys Gly Gly Ala Thr Ala Leu Met Asp Ala Ala Glu Lys Gly His

145 150 155 160

Val Glu Val Leu Lys Ile Leu Leu Asp Glu Met Gly Ala Asp Val Asn

165 170 175

Ala Cys Asp Asn Met Gly Arg Asn Ala Leu Ile His Ala Leu Leu Ser

180 185 190

Ser Asp Asp Ser Asp Val Glu Ala Ile Thr His Leu Leu Leu Asp His

195 200 205

Gly Ala Asp Val Asn Val Arg Gly Glu Arg Gly Lys Thr Pro Leu Ile

210 215 220

Leu Ala Val Glu Lys Lys His Leu Gly Leu Val Gln Arg Leu Leu Glu

225 230 235 240

Gln Glu His Ile Glu Ile Asn Asp Thr Asp Ser Asp Gly Lys Thr Ala

245 250 255

Leu Leu Leu Ala Val Glu Leu Lys Leu Lys Lys Ile Ala Glu Leu Leu

260 265 270

Cys Lys Arg Gly Ala Ser Thr Asp Cys Gly Asp Leu Val Met Thr Ala

275 280 285

Arg Arg Asn Tyr Asp His Ser Leu Val Lys Val Leu Leu Ser His Gly

290 295 300

Ala Lys Glu Asp Phe His Pro Pro Ala Glu Asp Trp Lys Pro Gln Ser

305 310 315 320

Ser His Trp Gly Ala Ala Leu Lys Asp Leu His Arg Ile Tyr Arg Pro

325 330 335

Met Ile Gly Lys Leu Lys Phe Phe Ile Asp Glu Lys Tyr Lys Ile Ala

340 345 350

Asp Thr Ser Glu Gly Gly Ile Tyr Leu Gly Phe Tyr Glu Lys Gln Glu

355 360 365

Val Ala Val Lys Thr Phe Cys Glu Gly Ser Pro Arg Ala Gln Arg Glu

370 375 380

Val Ser Cys Leu Gln Ser Ser Arg Glu Asn Ser His Leu Val Thr Phe

385 390 395 400

Tyr Gly Ser Glu Ser His Arg Gly His Leu Phe Val Cys Val Thr Leu

405 410 415

Cys Glu Gln Thr Leu Glu Ala Cys Leu Asp Val His Arg Gly Glu Asp

420 425 430

Val Glu Asn Glu Glu Asp Glu Phe Ala Arg Asn Val Leu Ser Ser Ile

435 440 445

Phe Lys Ala Val Gln Glu Leu His Leu Ser Cys Gly Tyr Thr His Gln

450 455 460

Asp Leu Gln Pro Gln Asn Ile Leu Ile Asp Ser Lys Lys Ala Ala His

465 470 475 480

Leu Ala Asp Phe Asp Lys Ser Ile Lys Trp Ala Gly Asp Pro Gln Glu

485 490 495

Val Lys Arg Asp Leu Glu Asp Leu Gly Arg Leu Val Leu Tyr Val Val

500 505 510

Lys Lys Gly Ser Ile Ser Phe Glu Asp Leu Lys Ala Gln Ser Asn Glu

515 520 525

Glu Val Val Gln Leu Ser Pro Asp Glu Glu Thr Lys Asp Leu Ile His

530 535 540

Arg Leu Phe His Pro Gly Glu His Val Arg Asp Cys Leu Ser Asp Leu

545 550 555 560

Leu Gly His Pro Phe Phe Trp Thr Trp Glu Ser Arg Tyr Arg Thr Leu

565 570 575

Arg Asn Val Gly Asn Glu Ser Asp Ile Lys Thr Arg Lys Ser Glu Ser

580 585 590

Glu Ile Leu Arg Leu Leu Gln Pro Gly Pro Ser Glu His Ser Lys Ser

595 600 605

Phe Asp Lys Trp Thr Thr Lys Ile Asn Glu Cys Val Met Lys Lys Met

610 615 620

Asn Lys Phe Tyr Glu Lys Arg Gly Asn Phe Tyr Gln Asn Thr Val Gly

625 630 635 640

Asp Leu Leu Lys Phe Ile Arg Asn Leu Gly Glu Asn Ile Asp Glu Glu

645 650 655

Lys His Lys Lys Met Lys Leu Lys Ile Gly Asp Pro Ser Leu Tyr Phe

660 665 670

Gln Lys Thr Phe Pro Asp Leu Val Ile Tyr Val Tyr Thr Lys Leu Gln

675 680 685

Asn Thr Glu Tyr Arg Lys His Phe Pro Gln Thr

690 695

<210> 28

<211> 104

<212> PRT

<213> 宏基因组(metagenomic)

<400> 28

Ala Cys Asp Tyr Thr Cys Gly Ser Asn Cys Tyr Ser Ser Ser Asp Val

1 5 10 15

Ser Thr Ala Gln Ala Ala Gly Tyr Lys Leu His Glu Asp Gly Glu Thr

20 25 30

Val Gly Ser Asn Ser Tyr Pro His Glu Phe Arg Asn Trp Asn Gly Phe

35 40 45

Asp Phe Ser Val Ser Ser Pro Tyr Tyr Glu Tyr Pro Ile Leu Ser Ser

50 55 60

Gly Asp Val Tyr Ser Gly Gly Ser Pro Gly Ala Asp Arg Val Val Phe

65 70 75 80

Asn Glu Asn Asn Gln Leu Ala Gly Val Ile Thr His Thr Gly Ala Ser

85 90 95

Gly Asn Asn Phe Val Glu Cys Thr

100

<210> 29

<211> 501

<212> PRT

<213> 宏基因组(metagenomic)

<400> 29

Gly Ala Gly His Met Ala Ser Pro Ser Glu Asn Leu Ser Arg Lys Ala

1 5 10 15

Lys Lys Lys Ala Ile Gln Gln Ser Pro Glu Ala Leu Leu Lys Gln Lys

20 25 30

Leu Asp Met Cys Ser Lys Lys Gly Asp Val Leu Glu Ala Leu Arg Leu

35 40 45

Tyr Asp Glu Ala Arg Arg Asn Gly Val Gln Leu Ser Gln Tyr His Tyr

50 55 60

Asn Val Leu Leu Tyr Val Cys Ser Leu Ala Glu Ala Ala Thr Glu Ser

65 70 75 80

Ser Pro Asn Pro Gly Leu Ser Arg Gly Phe Asp Ile Phe Lys Gln Met

85 90 95

Ile Val Asp Lys Val Val Pro Asn Glu Ala Thr Phe Thr Asn Gly Ala

100 105 110

Arg Leu Ala Val Ala Lys Asp Asp Pro Glu Met Ala Phe Asp Met Val

115 120 125

Lys Gln Met Lys Ala Phe Gly Ile Gln Pro Arg Leu Arg Ser Tyr Gly

130 135 140

Pro Ala Leu Phe Gly Phe Cys Arg Lys Gly Asp Ala Asp Lys Ala Tyr

145 150 155 160

Glu Val Asp Ala His Met Val Glu Ser Glu Val Val Pro Glu Glu Pro

165 170 175

Glu Leu Ala Ala Leu Leu Lys Val Ser Met Asp Thr Lys Asn Ala Asp

180 185 190

Lys Val Tyr Lys Thr Leu Gln Arg Leu Arg Asp Leu Val Arg Gln Val

195 200 205

Ser Lys Ser Thr Phe Asp Met Ile Glu Glu Trp Phe Lys Ser Glu Val

210 215 220

Ala Thr Lys Thr Gly Val Lys Lys Trp Asp Val Lys Lys Ile Arg Asp

225 230 235 240

Ala Val Val Ser Gly Gly Gly Gly Trp His Gly Gln Gly Trp Leu Gly

245 250 255

Thr Gly Lys Trp Asn Val Lys Arg Thr Glu Met Asp Glu Asn Gly Val

260 265 270

Cys Lys Cys Cys Lys Glu Lys Leu Val Cys Ile Asp Ile Asn Pro Val

275 280 285

Glu Thr Glu Thr Phe Ala Ala Ser Leu Thr Arg Leu Ala Cys Glu Arg

290 295 300

Glu Val Lys Ala Asn Phe Asn Gln Phe Gln Glu Trp Leu Glu Arg His

305 310 315 320

Gly Pro Phe Asp Ala Val Ile Asp Gly Ala Asn Met Gly Leu Val Asn

325 330 335

Gln Arg Ser Phe Ser Phe Phe Gln Leu Asn Asn Thr Val Gln Arg Cys

340 345 350

Gln Gln Ile Ser Pro Ser Lys Arg Leu Pro Leu Val Ile Leu His Lys

355 360 365

Ser Arg Val Asn Gly Gly Pro Ala Thr Tyr Pro Lys Asn Arg Ala Leu

370 375 380

Leu Glu Lys Trp Lys Asn Ala Gly Ala Leu Tyr Ala Thr Pro Pro Gly

385 390 395 400

Ser Asn Asp Asp Trp Tyr Trp Leu Tyr Ala Ala Val Ser Cys Lys Cys

405 410 415

Leu Leu Val Thr Asn Asp Glu Met Arg Asp His Leu Phe Gln Leu Leu

420 425 430

Gly Asn Ser Phe Phe Pro Arg Trp Lys Glu Lys His Gln Val Arg Ile

435 440 445

Ser Val Thr Arg Glu Asp Gly Leu Lys Leu Asn Met Pro Pro Pro Tyr

450 455 460

Ser Ile Val Ile Gln Glu Ser Glu Asp Gly Thr Trp His Val Pro Met

465 470 475 480

Ser Val Glu Asp Asp Leu Gln Thr Ser Arg Gln Trp Leu Cys Ala Lys

485 490 495

Arg Ser Lys Thr Pro

500

<210> 30

<211> 96

<212> PRT

<213> 宏基因组(metagenomic)

<400> 30

Gly Ala His Arg Gly Arg Val Thr Arg Arg Asn Ile Ile Trp His Glu

1 5 10 15

Leu Ile Gly Leu Arg Val Arg Ile Val Gly Ser Thr His Pro Ala Phe

20 25 30

Val Gly Ile Glu Gly Tyr Val Ile Asp Glu Thr Arg Asn Met Leu Val

35 40 45

Ile Ala Gly Asp Arg Ile Trp Lys Val Pro Lys Asp Val Ser Ile Phe

50 55 60

Glu Phe Glu Ala Asp Asp Gly Thr Lys Ile Lys Ile Pro Gly Glu Arg

65 70 75 80

Leu Val Gly Arg Pro Glu Met Arg Leu Lys Lys Arg Trp Lys Lys Trp

85 90 95

<210> 31

<211> 165

<212> PRT

<213> 宏基因组(metagenomic)

<400> 31

Gly Ala Met Gly Gln Tyr Glu Gly Val Phe Tyr Thr Asp Gly Ser Ala

1 5 10 15

Ile Lys Ser Pro Asp Pro Thr Lys Ser Asn Asn Ala Gly Met Gly Ile

20 25 30

Val His Ala Thr Tyr Lys Pro Glu Tyr Gln Val Leu Asn Gln Trp Ser

35 40 45

Ile Pro Leu Gly Asn His Thr Ala Gln Met Ala Glu Ile Ala Ala Val

50 55 60

Glu Phe Ala Cys Lys Lys Ala Leu Lys Ile Pro Gly Pro Val Leu Val

65 70 75 80

Ile Thr Asp Ser Phe Tyr Val Ala Glu Ser Ala Asn Lys Glu Leu Pro

85 90 95

Tyr Trp Lys Ser Asn Gly Phe Val Asn Asn Lys Lys Lys Pro Leu Lys

100 105 110

His Ile Ser Lys Trp Lys Ser Ile Ala Glu Cys Leu Ser Met Lys Pro

115 120 125

Asp Ile Thr Ile Gln His Glu Lys Gly His Gln Pro Thr Asn Thr Ser

130 135 140

Ile His Thr Glu Gly Asn Ala Leu Ala Asp Lys Leu Ala Thr Gln Gly

145 150 155 160

Ser Tyr Val Val Asn

165

<210> 32

<211> 27

<212> RNA

<213> 宏基因组(metagenomic)

<400> 32

ggaagaccgg ucuucggacc ggcuucc 27

<210> 33

<211> 27

<212> RNA

<213> 宏基因组(metagenomic)

<400> 33

ggaagcucgg ucuucggacc ggcuucc 27

<210> 34

<211> 46

<212> RNA

<213> 宏基因组(metagenomic)

<400> 34

ggacucagua auaugcuuug gaaacgaagc uuacaaaaug gagucc 46

<210> 35

<211> 347

<212> RNA

<213> 宏基因组(metagenomic)

<400> 35

ggagaggagc aggcggucgc gggggcgcac accugcgcuu cccgaggaaa guccggacuc 60

uggagcgggg ugccggguaa cgcccgggag gggugacccu cggacagggc cauagagaag 120

aagaccgccc ggggggaaac uuccgggcaa ggguggaacg gugggguaag agcccaccag 180

cgucggggca acccggcggc uuggcaaccc ccaccuggag caaggccaag caggggguug 240

ggucgcuccc ccuauucccc cggguuggcc gcuugaggug ugcgguaacg cacaccccag 300

auugaugacc gcccacgaca gaauccggcu uaugcuccuc ucccgug 347

<210> 36

<211> 338

<212> RNA

<213> 宏基因组(metagenomic)

<400> 36

ggagaggagc aggcggucgc gggggcgcac accugcgcuu cccgaggaaa guccggacuc 60

uggagcgggg ugccggguaa cgcccgggag gggugacccu cggacagggc cauagagaag 120

aagaccgccc guugagggca aggguggaac ggugggguaa gagcccacca gcgucggggc 180

aacccggcgg cuuggcaacc cccaccugga gcaaggccaa gcaggggguu gggucgcucc 240

cccuauuccc ccggguuggc cgcuugaggu gugcgguaac gcacacccca gauugaugac 300

cgcccacgac agaauccggc uuaugcuccu cucccgug 338

<210> 37

<211> 284

<212> PRT

<213> 宏基因组(metagenomic)

<400> 37

Met Val Leu Met Ile Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val

1 5 10 15

Ala Leu Arg Ala Leu Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu

20 25 30

Pro Val Val Leu Leu Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu

35 40 45

Ile Ser Ala Ala Val Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro

50 55 60

Glu Ile Phe Glu Gln Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro

65 70 75 80

Gln Leu Leu Phe Leu Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr

85 90 95

Ser Asp Thr Arg Arg Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu

100 105 110

Glu Ser Ala Ile Asp Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser

115 120 125

Arg Ala Asp Leu Ile Val Asp Thr Ser Glu Met Ser Val His Glu Leu

130 135 140

Ala Glu Met Leu Arg Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu

145 150 155 160

Thr Met Val Phe Glu Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp

165 170 175

Ala Asp Tyr Val Phe Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp

180 185 190

Pro Lys Leu Arg Pro Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe

195 200 205

Leu Asp Arg His Thr Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser

210 215 220

Tyr Leu Glu Leu Trp Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr

225 230 235 240

Leu Thr Val Ala Ile Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr

245 250 255

Ile Ala Glu Gln Leu Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val

260 265 270

Gln Ser Arg His Arg Thr Leu Glu Lys Arg Lys Pro

275 280

<210> 38

<211> 165

<212> PRT

<213> 宏基因组(metagenomic)

<400> 38

Gly Ser Ile Leu Ala Glu Thr His Gly Thr Arg Pro Asp Leu Thr Asp

1 5 10 15

Gln Pro Ile Pro Asp Ala Asp Tyr Thr Trp Tyr Thr Asp Gly Ser Ser

20 25 30

Phe Leu Gln Glu Gly Gln Arg Arg Ala Gly Ala Ala Val Thr Thr Glu

35 40 45

Thr Glu Val Ile Trp Ala Arg Ala Leu Pro Ala Gly Thr Ser Ala Gln

50 55 60

Arg Ala Glu Leu Ile Ala Leu Thr Gln Ala Leu Lys Met Ala Glu Gly

65 70 75 80

Lys Lys Leu Asn Val Tyr Thr Asp Ser Arg Tyr Ala Phe Ala Thr Ala

85 90 95

His Val His Ser Glu Gly Arg Glu Ile Lys Asn Lys Asn Glu Ile Leu

100 105 110

Ala Leu Leu Lys Ala Leu Phe Leu Pro Lys Arg Leu Ser Ile Ile His

115 120 125

Cys Pro Gly His Gln Lys Gly Asn Ser Ala Glu Ala Arg Gly Asn Arg

130 135 140

Met Ala Asp Gln Ala Ala Arg Glu Ala Ala Met Lys Ala Val Leu Glu

145 150 155 160

Thr Ser Thr Leu Leu

165

<210> 39

<211> 123

<212> PRT

<213> 宏基因组(metagenomic)

<400> 39

Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala

1 5 10 15

Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr His His Pro

20 25 30

Tyr Ile Tyr Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu

35 40 45

Gly Val Ala Ala Met Asp Ser Gly Gly Gly Gly Thr Leu Tyr Ala Asp

50 55 60

Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Lys Gly Lys Asn Thr

65 70 75 80

Val Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Thr Tyr

85 90 95

Tyr Cys Ala Ala Gly Gly His His Leu Arg Asp His Thr Tyr Gly Gln

100 105 110

Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser

115 120

<210> 40

<211> 268

<212> RNA

<213> 宏基因组(metagenomic)

<400> 40

guuaaucaug cucggguaau cgcugcggcc gguuucggcc guagaggaaa guccaugcuc 60

gcacggugcu gagaugcccg uaguguucgu ggaaacacga gcgagaaacc caaaugaugg 120

uaggggcacc uucccgaagg aaaugaacgg agggaaggac aggcggcgca ugcagccugu 180

agauagauga uuaccgccgg aguacgaggc gcaaagccgc uugcaguacg aagguacaga 240

acauggcuua uagagcauga uuaacguc 268

<210> 41

<211> 231

<212> PRT

<213> 宏基因组(metagenomic)

<400> 41

Lys His Pro Pro Leu Pro Phe Ile Lys Asp Gln Thr Leu Tyr Glu Arg

1 5 10 15

Val Phe Val His Lys Ser Val Val Asn Gly Lys Thr Tyr Leu Asp Gln

20 25 30

Asn Asp Leu Ile Asn Ser His Asn Glu Arg Leu Glu Phe Leu Gly Asp

35 40 45

Ser Val Leu Asn Asn Leu Val Thr Leu Ile Ile Tyr Asp Lys Phe Pro

50 55 60

Ser Ala Ser Glu Gly Lys Leu Thr Lys Met Arg Ser Gln Leu Ile Asp

65 70 75 80

Asn His Thr Leu Thr Gln Phe Ser Phe Glu Tyr Gly Phe Asp Lys Arg

85 90 95

Leu Lys Thr Lys Thr Asp Glu Glu Ile Leu Lys Thr Gly Asp Gln Lys

100 105 110

Val Tyr Ala Asp Ile Phe Glu Ala Tyr Ile Gly Ala Leu Ser Val Glu

115 120 125

Arg Gly Leu Asp Leu Arg Glu Ile Lys Asp Trp Leu Glu Lys Leu Tyr

130 135 140

Ala Pro Lys Leu Glu Ala Phe Lys Val Asn Phe Leu Gln Glu Ser Val

145 150 155 160

Asn Lys Glu Ala Lys Ser Glu Leu Tyr Ser Ile Val Gly Thr Ala Ser

165 170 175

Ser His Pro Leu Tyr Val Val Val Glu Glu Gly Asn Gly Ser His Asp

180 185 190

Phe Val Val Glu Cys Arg Met Gly Asn Asp Val Leu Gly Arg Ala Lys

195 200 205

Ala Pro Ser Gln Lys Glu Ala Gly Leu Arg Ala Ala Met Asp Ala Leu

210 215 220

Lys Asn Arg Gln Leu Leu Glu

225 230

<210> 42

<211> 267

<212> PRT

<213> 宏基因组(metagenomic)

<400> 42

Met Ala Ala Leu Thr Pro Ser Arg Ser Val Phe Ala Ser Thr Cys Lys

1 5 10 15

Thr Ile Ala Gln Gln Cys Ser Arg Arg Pro Val Ala Val Gln Gln Leu

20 25 30

Ala Ala Val Pro Val Ala Arg Gln Val Ser Ser Ser Ser Ala Val Gln

35 40 45

Gln Glu Gln Asp Ala Ser Gly Thr Ser Ser Ser Gln Gln Pro Arg Pro

50 55 60

Arg Trp Ser Tyr Thr Pro Glu Arg Met Lys Gly Pro Gly Phe Ser Leu

65 70 75 80

Asn Leu Val Lys Asp Pro Arg Arg Lys Gln Trp Met Val Asn Ser Asp

85 90 95

Pro Ala Lys Leu Asp Ala Phe Tyr Asp Ala Phe Leu Gly Gln Gly Gly

100 105 110

Ser Arg Met Leu Ser Glu Glu Thr Lys Trp Leu Ala Val Thr His Lys

115 120 125

Ser Phe Asp Tyr Gly Arg Arg Gly Tyr Asn Thr Arg Leu Ala Phe Leu

130 135 140

Gly Arg Gln Ile Ile Ala Leu Glu Thr Thr Arg Ser Ile Leu Thr Ser

145 150 155 160

Pro Val Leu Asn Glu Pro Ile Val Asp Lys Tyr Gly Arg Thr Pro Tyr

165 170 175

Asn His Ala Ala Leu Ala Asn Ile Asp Lys Leu Ile Tyr Thr Gln Pro

180 185 190

Leu Asp Ile Met Asp Lys Thr Lys Ile Ala Arg Met Gly Ile Asp Phe

195 200 205

Gly Leu Leu Thr Val Met Arg Trp Lys Pro Arg Met Pro Glu Asp Leu

210 215 220

Glu Ser Ser Gly Val Val Val Val Leu Asn Ser Thr Leu Phe Ala Ile

225 230 235 240

Ile Gly Ala Ile Ser Leu Glu Lys Gly Ala Ala Val Ala Gln Arg Ile

245 250 255

Val Arg Glu Lys Ile Leu Lys Arg Leu Gly Ala

260 265

<210> 43

<211> 221

<212> PRT

<213> 宏基因组(metagenomic)

<400> 43

Met Ala Tyr Val Gly Gln Ser Arg Asn Val Ile Arg His Val Val Ser

1 5 10 15

Arg Gly Thr Ala Tyr His Lys Tyr Glu Asn Ala Ile His His Pro Leu

20 25 30

Leu Phe Ala Cys Gln Gly Val Arg Tyr Arg Lys Leu Glu Val Ile Leu

35 40 45

Thr Thr Gly Ile Glu Lys Leu Gly Lys Ala Gly Glu Thr Val Lys Val

50 55 60

Ala Pro Gly Tyr Phe Arg Asn His Leu Met Pro Lys Leu Leu Ala Val

65 70 75 80

Pro Asn Ile Asp Lys Tyr Ala Tyr Leu Ile Arg Glu Gln Arg Lys Met

85 90 95

Tyr Asn His Glu Glu Val Lys Glu Glu Val Lys Val Val His Lys Thr

100 105 110

Ser Glu Val Gln Thr Lys Glu Tyr Glu Lys Ala Ala Lys Arg Leu Ala

115 120 125

Asn Ala Asn Leu Val Leu Arg Lys Leu Val Asp Lys Glu Lys Phe Lys

130 135 140

Asn Arg Ser Ser Lys Asp Asp Lys Pro Asp Val Gln Thr Pro Ile Thr

145 150 155 160

Lys Glu Glu Ile Val Ser Glu Val Ala Arg Gln Leu Cys Val Lys Ile

165 170 175

Asp Pro Asp Asn Val Val Leu Ala Ala Pro Leu Glu Thr Phe Gly Glu

180 185 190

Tyr Glu Val Pro Leu Lys Phe Pro Lys Thr Ile Pro Leu Pro Gln Gly

195 200 205

Thr Val Gln Trp Ile Leu Lys Val Lys Val Arg Gly His

210 215 220

<210> 44

<211> 279

<212> PRT

<213> 宏基因组(metagenomic)

<400> 44

Met Asp Arg Thr Gln Thr Phe Ile Lys Asp Cys Leu Phe Thr Lys Cys

1 5 10 15

Leu Glu Asp Pro Glu Lys Pro Phe Asn Glu Asn Arg Phe Gln Asp Thr

20 25 30

Leu Leu Leu Leu Pro Thr Asp Gly Gly Leu Thr Ser Arg Leu Gln Arg

35 40 45

Gln Gln Arg Lys Ser Lys Leu Asn Leu Asp Asn Leu Gln Lys Val Ser

50 55 60

Gln Leu Glu Ser Ala Asp Lys Gln Leu Glu Lys Arg Asp Tyr Gln Arg

65 70 75 80

Ile Asn Lys Asn Ser Lys Ile Ala Leu Arg Glu Tyr Ile Asn Asn Cys

85 90 95

Lys Lys Asn Thr Lys Lys Cys Leu Lys Leu Ala Tyr Glu Asn Lys Ile

100 105 110

Thr Asp Lys Glu Asp Leu Leu His Tyr Ile Glu Glu Lys His Pro Thr

115 120 125

Ile Tyr Glu Ser Leu Pro Gln Tyr Val Asp Phe Val Pro Met Tyr Lys

130 135 140

Glu Leu Trp Ile Asn Tyr Ile Lys Glu Leu Leu Asn Ile Thr Lys Asn

145 150 155 160

Leu Lys Thr Phe Asn Gly Ser Leu Ala Leu Leu Lys Leu Ser Met Ala

165 170 175

Asp Tyr Asn Gly Ala Leu Leu Arg Val Thr Lys Ser Lys Asn Lys Thr

180 185 190

Leu Ile Gly Leu Gln Gly Ile Val Ile Trp Asp Ser Gln Lys Phe Phe

195 200 205

Ile Met Ile Val Lys Gly Asn Ile Ile Asp Glu Ile Lys Cys Ile Pro

210 215 220

Lys Lys Gly Thr Val Phe Gln Phe Glu Ile Pro Ile Ser Asp Asp Asp

225 230 235 240

Asp Ser Ala Leu Arg Tyr Ser Ile Leu Gly Asp Arg Phe Lys Tyr Arg

245 250 255

Ser Val Asp Arg Ala Gly Arg Lys Phe Lys Ser Arg Arg Cys Asp Asp

260 265 270

Met Leu Tyr Tyr Ile Gln Asn

275

<210> 45

<211> 538

<212> PRT

<213> 宏基因组(metagenomic)

<400> 45

Met Glu Gly Glu Val Ile His Arg Tyr Lys Val Asn Gly Phe Lys Leu

1 5 10 15

Phe Gly Leu Pro Thr Pro Lys Asn Asn Thr Ile Leu Gly Val Leu Gly

20 25 30

Lys Asn Gly Val Gly Lys Thr Thr Val Leu Lys Ile Leu Ala Gly Glu

35 40 45

Ile Ile Pro Asn Phe Gly Asp Pro Asn Ser Lys Val Gly Lys Asp Glu

50 55 60

Val Leu Lys Arg Phe Arg Gly Lys Glu Ile Tyr Asn Tyr Phe Lys Glu

65 70 75 80

Leu Tyr Ser Asn Glu Leu Lys Ile Val His Lys Ile Gln Tyr Val Glu

85 90 95

Tyr Ala Ser Lys Phe Leu Lys Gly Thr Val Asn Glu Ile Leu Thr Lys

100 105 110

Ile Asp Glu Arg Gly Lys Lys Asp Glu Val Lys Glu Leu Leu Asn Met

115 120 125

Thr Asn Leu Trp Asn Lys Asp Ala Asn Ile Leu Ser Gly Gly Gly Leu

130 135 140

Gln Arg Leu Leu Val Ala Ala Ser Leu Leu Arg Glu Ala Asp Val Tyr

145 150 155 160

Ile Phe Asp Gln Pro Ser Ser Tyr Leu Asp Val Arg Glu Arg Met Asn

165 170 175

Met Ala Lys Ala Ile Arg Glu Leu Leu Lys Asn Lys Tyr Val Ile Val

180 185 190

Val Asp His Asp Leu Ile Val Leu Asp Tyr Leu Thr Asp Leu Ile His

195 200 205

Ile Ile Tyr Gly Glu Ser Ser Val Tyr Gly Arg Val Ser Lys Ser Tyr

210 215 220

Ala Ala Arg Val Gly Ile Asn Asn Phe Leu Lys Gly Tyr Leu Pro Ala

225 230 235 240

Glu Asn Met Lys Ile Arg Pro Asp Glu Ile Lys Phe Met Leu Lys Glu

245 250 255

Val Ser Asp Leu Asp Leu Ser Lys Asp Leu Lys Thr Lys Met Lys Trp

260 265 270

Thr Lys Ile Ile Lys Lys Leu Gly Asp Phe Gln Leu Val Val Asp Asn

275 280 285

Gly Glu Ala Lys Glu Gly Glu Ile Ile Gly Ile Leu Gly Pro Asn Gly

290 295 300

Ile Gly Lys Thr Thr Phe Ala Arg Ile Leu Val Gly Glu Ile Thr Ala

305 310 315 320

Asp Glu Gly Ser Val Thr Pro Glu Lys Gln Ile Leu Ser Tyr Lys Pro

325 330 335

Gln Arg Ile Phe Pro Asn Tyr Asp Gly Thr Val Gln Gln Tyr Leu Glu

340 345 350

Asn Ala Ser Lys Asp Ala Leu Ser Thr Ser Ser Trp Phe Phe Glu Glu

355 360 365

Val Thr Lys Arg Leu Asn Leu His Arg Leu Leu Glu Ser Asn Val Asn

370 375 380

Asp Leu Ser Gly Gly Glu Leu Gln Lys Leu Tyr Ile Ala Ala Thr Leu

385 390 395 400

Ala Lys Glu Ala Asp Leu Tyr Val Leu Asp Gln Pro Ser Ser Tyr Leu

405 410 415

Asp Val Glu Glu Arg Tyr Ile Val Ala Lys Ala Ile Lys Arg Val Thr

420 425 430

Arg Glu Arg Lys Ala Val Thr Phe Ile Ile Asp His Asp Leu Ser Ile

435 440 445

His Asp Tyr Ile Ala Asp Arg Ile Ile Val Phe Lys Gly Glu Pro Glu

450 455 460

Lys Ala Gly Leu Ala Thr Ser Pro Val Thr Leu Lys Thr Gly Met Asn

465 470 475 480

Glu Phe Leu Arg Glu Leu Glu Val Thr Phe Arg Arg Asp Ala Glu Thr

485 490 495

Gly Arg Pro Arg Val Asn Lys Ile Gly Ser Tyr Leu Asp Arg Val Gln

500 505 510

Lys Glu Arg Gly Asp Tyr Tyr Ser Met Val Leu Ser Thr Gln Gly Ser

515 520 525

Ile Glu Gly Arg His His His His His His

530 535

<210> 46

<211> 165

<212> PRT

<213> 宏基因组(metagenomic)

<400> 46

Met Gly His His His His His His Gly Gly Met Ile Thr Tyr Gly Ser

1 5 10 15

Gly Asp Leu Leu Arg Ala Asp Thr Glu Ala Leu Val Asn Thr Val Asn

20 25 30

Cys Val Gly Val Met Gly Lys Gly Ile Ala Leu Gln Phe Lys Arg Arg

35 40 45

Tyr Pro Glu Met Phe Thr Ala Tyr Glu Lys Ala Cys Lys Arg Gly Glu

50 55 60

Val Thr Ile Gly Lys Met Phe Val Val Asp Thr Gly Gln Leu Asp Gly

65 70 75 80

Pro Lys His Ile Ile Asn Phe Pro Thr Lys Lys His Trp Arg Ala Pro

85 90 95

Ser Lys Leu Ala Tyr Ile Asp Ala Gly Leu Ile Asp Leu Ile Arg Val

100 105 110

Ile Arg Glu Leu Asn Ile Ala Ser Val Ala Val Pro Pro Leu Gly Val

115 120 125

Gly Asn Gly Gly Leu Asp Trp Glu Asp Val Glu Gln Arg Leu Val Ser

130 135 140

Ala Phe Gln Gln Leu Pro Asp Val Asp Ala Val Ile Tyr Pro Pro Ser

145 150 155 160

Gly Gly Ser Arg Ala

165

<210> 47

<211> 470

<212> PRT

<213> 宏基因组(metagenomic)

<400> 47

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro

1 5 10 15

Arg Gly Ser His Met Ala Ser Thr Glu Ile Gly Ile Ile Ala Val Gly

20 25 30

Gly Tyr Asn Glu Met Gly Arg Asn Met Thr Ala Ile Arg Val Asn Glu

35 40 45

Asp Ile Ile Ile Ile Asp Met Gly Ile Arg Leu Asp Arg Val Gln Ile

50 55 60

His Glu Asp Val Asp Thr Asp Arg Met His Ser Leu Glu Leu Ile Glu

65 70 75 80

Met Gly Ala Ile Pro Asp Asp Thr Ile Met Asn Glu Val Asn Gly Asn

85 90 95

Val Arg Ala Ile Val Cys Thr His Gly His Leu Asp His Ile Gly Ala

100 105 110

Ile Pro Lys Leu Ala His Arg Tyr Ala Ala Pro Ile Ile Ala Thr Pro

115 120 125

Tyr Thr Thr Ala Leu Ile Lys His Gln Ile Asp Ser Glu Arg Lys Phe

130 135 140

Gly Val Lys Asn Asn Ile Val Ala Leu Lys Ala Gly Glu Thr Leu Glu

145 150 155 160

Ile Thr Lys Asp Ile Thr Ile Glu Phe Ile Asn Thr Gln His Ser Ile

165 170 175

Ile Asp Thr Val Phe Val Ala Ile His Thr Pro Ser Gly Ala Val Val

180 185 190

Tyr Ala Cys Asp Phe Lys Phe Asp Arg Thr Pro Thr Leu Gly Glu Val

195 200 205

Pro Asp Phe Asp Arg Leu Lys Glu Leu Gly Lys Glu Gly Val Ile Ala

210 215 220

Leu Ile Thr Glu Ser Thr Asn Ala Gly Arg Asn Gly Lys Thr Pro Ser

225 230 235 240

Glu Leu Ile Ala His Met Met Leu Lys Asp Val Leu Leu Gly Thr Glu

245 250 255

Glu Ser Ala Val Gly Met Ile Val Thr Thr Phe Ala Ala His Ile Ala

260 265 270

Arg Val Asn Ser Ile Val Gln Phe Ala Gln Glu Met Gly Arg Ile Pro

275 280 285

Val Leu Leu Gly Arg Ser Met Glu Arg Tyr Val Gly Thr Ala Tyr Gln

290 295 300

Leu Gly Tyr Ile Asp Leu Pro Glu Asn Val Glu Ile Tyr Gly Ser Arg

305 310 315 320

Arg Asp Ile Asp Asn Ala Leu Lys Lys Ile Met Glu Ala Gly Lys Asp

325 330 335

Lys Tyr Leu Pro Val Met Thr Gly His Gln Gly Glu Pro Gly Ala Val

340 345 350

Leu Gly Arg Ile Ala Asn Gly Glu Thr Pro Phe Lys Val Glu Thr Gly

355 360 365

Asp Arg Ile Ile Phe Ser Ala Asn Val Ile Pro Asn Pro Met Thr Gln

370 375 380

Ala Asn Arg Tyr Ala Leu Glu Thr Lys Leu Lys Met Lys Gly Ala Arg

385 390 395 400

Ile Tyr Asp Asn Val His Val Ser Gly His Ala Tyr Arg Glu Asp His

405 410 415

Trp Glu Leu Leu Arg Met Leu Lys Pro Glu His Val Ile Pro Ala His

420 425 430

Gly Thr Ile Gln Met His Ser Glu Tyr Ile Gln Met Ala Glu Asp Ala

435 440 445

Gly Tyr Ser Leu Gly Asp Thr Leu His Leu Leu Arg Asn Gly Glu Glu

450 455 460

Leu Tyr Ile Glu Glu Asp

465 470

<210> 48

<211> 538

<212> PRT

<213> 宏基因组(metagenomic)

<400> 48

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro

1 5 10 15

Arg Ser His Met Glu Gln Leu Glu Glu Asp Cys Val His Arg Tyr Gly

20 25 30

Val Asn Ala Phe Val Leu Tyr Arg Leu Pro Val Val Lys Glu Gly Met

35 40 45

Val Val Gly Ile Val Gly Pro Asn Gly Thr Gly Lys Ser Thr Ala Val

50 55 60

Lys Ile Leu Ala Gly Gln Leu Ile Pro Asn Leu Cys Gly Asp Asn Asp

65 70 75 80

Ser Trp Asp Gly Val Ile Arg Ala Phe Arg Gly Asn Glu Leu Gln Asn

85 90 95

Tyr Phe Glu Lys Leu Lys Asn Gly Glu Ile Arg Pro Val Val Lys Pro

100 105 110

Gln Tyr Val Asp Leu Ile Pro Lys Ala Val Lys Gly Lys Val Ile Glu

115 120 125

Leu Leu Lys Lys Ala Asp Glu Thr Gly Lys Leu Glu Glu Val Val Lys

130 135 140

Ala Leu Glu Leu Glu Asn Val Leu Glu Arg Glu Ile Gln His Leu Ser

145 150 155 160

Gly Gly Glu Leu Gln Arg Val Ala Ile Ala Ala Ala Leu Leu Arg Asn

165 170 175

Ala Thr Phe Tyr Phe Phe Asp Glu Pro Ser Ser Tyr Leu Asp Ile Arg

180 185 190

Gln Arg Leu Asn Ala Ala Arg Ala Ile Arg Arg Leu Ser Glu Glu Gly

195 200 205

Lys Ser Val Leu Val Val Glu His Asp Leu Ala Val Leu Asp Tyr Leu

210 215 220

Ser Asp Ile Ile His Val Val Tyr Gly Glu Pro Gly Val Tyr Gly Ile

225 230 235 240

Phe Ser Gln Pro Lys Gly Thr Arg Asn Gly Ile Asn Glu Phe Leu Arg

245 250 255

Gly Tyr Leu Lys Asp Glu Asn Val Arg Phe Arg Pro Tyr Glu Ile Lys

260 265 270

Phe Thr Lys Thr Gly Glu Arg Val Glu Ile Glu Arg Glu Thr Leu Val

275 280 285

Thr Tyr Pro Arg Leu Val Lys Asp Tyr Gly Ser Phe Arg Leu Glu Val

290 295 300

Glu Pro Gly Glu Ile Lys Lys Gly Glu Val Ile Gly Ile Val Gly Pro

305 310 315 320

Asn Gly Ile Gly Lys Thr Thr Phe Val Lys Met Leu Ala Gly Val Glu

325 330 335

Glu Pro Thr Glu Gly Lys Ile Glu Trp Asp Leu Thr Val Ala Tyr Lys

340 345 350

Pro Gln Tyr Ile Lys Ala Asp Tyr Glu Gly Thr Val Tyr Glu Leu Leu

355 360 365

Ser Lys Ile Asp Ala Ser Lys Leu Asn Ser Asn Phe Tyr Lys Thr Glu

370 375 380

Leu Leu Lys Pro Leu Gly Ile Ile Asp Leu Tyr Asp Arg Glu Val Asn

385 390 395 400

Glu Leu Ser Gly Gly Glu Leu Gln Arg Val Ala Ile Ala Ala Thr Leu

405 410 415

Leu Arg Asp Ala Asp Ile Tyr Leu Leu Asp Glu Pro Ser Ala Tyr Leu

420 425 430

Asp Val Glu Gln Arg Leu Ala Val Ser Arg Ala Ile Arg His Leu Met

435 440 445

Glu Lys Asn Glu Lys Thr Ala Leu Val Val Glu His Asp Val Leu Met

450 455 460

Ile Asp Tyr Val Ser Asp Arg Leu Met Val Phe Glu Gly Glu Pro Gly

465 470 475 480

Lys Tyr Gly Arg Ala Leu Pro Pro Met Gly Met Arg Glu Gly Met Asn

485 490 495

Arg Phe Leu Ala Ser Ile Gly Ile Thr Phe Arg Arg Asp Pro Asp Thr

500 505 510

Gly Arg Pro Arg Ala Asn Lys Glu Gly Ser Val Lys Asp Arg Glu Gln

515 520 525

Lys Glu Lys Gly Glu Tyr Tyr Tyr Ile Ala

530 535

<210> 49

<211> 572

<212> PRT

<213> 宏基因组(metagenomic)

<400> 49

Met Gly Ser Ser His His His His His His Ser Ser Pro Ile Ser Pro

1 5 10 15

Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met Asp Gly Pro Lys

20 25 30

Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys Ala Leu Val Glu

35 40 45

Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser Lys Ile Gly Pro

50 55 60

Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys Lys Lys Asp Ser

65 70 75 80

Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu Asn Lys Arg Thr

85 90 95

Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His Pro Ala Gly Leu

100 105 110

Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val Gly Asp Ala Tyr Phe

115 120 125

Ser Val Pro Leu Asp Glu Asp Phe Arg Lys Tyr Thr Ala Phe Thr Ile

130 135 140

Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr Gln Tyr Asn Val

145 150 155 160

Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe Gln Ser Ser Met

165 170 175

Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro Asp Ile Val Ile

180 185 190

Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp Leu Glu Ile Gly

195 200 205

Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln His Leu Leu Arg Trp

210 215 220

Gly Leu Thr Thr Pro Asp Lys Lys His Gln Lys Glu Pro Pro Phe Leu

225 230 235 240

Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr Val Gln Pro Ile

245 250 255

Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp Ile Cys Lys Leu

260 265 270

Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Pro Gly Ile Lys Val

275 280 285

Arg Gln Leu Ser Lys Leu Leu Arg Gly Thr Lys Ala Leu Thr Glu Val

290 295 300

Ile Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala Glu Asn Arg Glu

305 310 315 320

Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp Pro Ser Lys Asp

325 330 335

Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln Trp Thr Tyr Gln

340 345 350

Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly Lys Tyr Ala Arg

355 360 365

Met Arg Gly Ala His Thr Asn Asp Val Lys Gln Leu Thr Glu Ala Val

370 375 380

Gln Lys Ile Thr Thr Glu Ser Ile Val Ile Trp Gly Lys Thr Pro Lys

385 390 395 400

Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Thr Trp Trp Thr Glu

405 410 415

Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe Val Asn Thr Pro

420 425 430

Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu Pro Ile Val Gly

435 440 445

Ala Glu Thr Phe Tyr Val Asp Gly Ala Ala Asn Arg Glu Thr Lys Leu

450 455 460

Gly Lys Ala Gly Tyr Val Thr Asn Arg Gly Arg Gln Lys Val Val Thr

465 470 475 480

Leu Thr Asp Thr Thr Asn Gln Lys Thr Glu Leu Gln Ala Ile Tyr Leu

485 490 495

Ala Leu Gln Asp Ser Gly Leu Glu Val Asn Ile Val Thr Asp Ser Gln

500 505 510

Tyr Ala Leu Gly Ile Ile Gln Ala Gln Pro Asp Gln Ser Glu Ser Glu

515 520 525

Leu Val Asn Gln Ile Ile Glu Gln Leu Ile Lys Lys Glu Lys Val Tyr

530 535 540

Leu Ala Trp Val Pro Ala His Lys Gly Ile Gly Gly Asn Glu Gln Val

545 550 555 560

Asp Lys Leu Val Ser Ala Gly Ile Arg Lys Val Leu

565 570

<210> 50

<211> 126

<212> PRT

<213> 宏基因组(metagenomic)

<400> 50

Met His Val Lys Glu Arg Tyr Lys Asn Phe Leu Asn Gln His Val Gly

1 5 10 15

Pro Asp Met Ser Val Gln Arg Cys Asn Ser Glu Ile Gly Pro Asn Asn

20 25 30

Arg Lys Ile Thr Leu Ser Gly Thr Asp Asn Gly Cys Lys Pro Val Asn

35 40 45

Thr Phe Ile Leu Ala Asn Lys Arg Leu Ile Lys Thr Val Cys Gly Arg

50 55 60

Ala Gly Ser Pro Gln Gly Asn Met Val Arg Ser Asn Gln Pro Phe Pro

65 70 75 80

Val Val Lys Cys Val Leu Asn Asn Gly Glu Arg His Pro Tyr Cys Glu

85 90 95

Tyr Arg Gly Thr Arg Ser Thr Arg Tyr Ile Val Leu Lys Cys Glu Glu

100 105 110

Gly Trp Pro Val His Tyr His Glu Asp Glu Val Asn Val Gly

115 120 125

<210> 51

<211> 130

<212> PRT

<213> 宏基因组(metagenomic)

<400> 51

Met Lys Val Pro Pro Asp Val Asp Pro Arg Tyr Gln Lys Phe Leu Arg

1 5 10 15

Gln His Val Asp Ala Asp Met Ser Val Gln Lys Cys Asp Arg Ala Met

20 25 30

Ser Ile Lys Lys Ile Thr Ala Gly Thr Gly Asn Asp Cys Lys Glu Val

35 40 45

Asn Thr Phe Ile Gln Ala Thr Lys Asp Arg Ile Thr Thr Val Cys Gly

50 55 60

Asp Ala Gly Thr Pro Val Asn Asn Leu Phe Lys Ser Asn Gln Pro Phe

65 70 75 80

Pro Val Val Thr Cys Lys Leu Lys Ser Gly Asn Arg Arg Pro Asn Cys

85 90 95

Gln Tyr Arg Gly Thr Ser Ser Thr Arg Tyr Ile Val Leu Gly Cys Asp

100 105 110

Lys Gly Trp Pro Val His Tyr Asp Glu Gly Ile Ile Asp Val Asn Arg

115 120 125

Ser Gly

130

<210> 52

<211> 280

<212> PRT

<213> 宏基因组(metagenomic)

<400> 52

Met Asn Ile Ile Gly Phe Ser Lys Ala Leu Phe Ser Thr Trp Ile Tyr

1 5 10 15

Tyr Ser Pro Glu Arg Ile Leu Phe Asp Ala Gly Glu Gly Val Ser Thr

20 25 30

Thr Leu Gly Ser Lys Val Tyr Ala Phe Lys Tyr Val Phe Leu Thr His

35 40 45

Gly His Val Asp His Ile Ala Gly Leu Trp Gly Val Val Asn Ile Arg

50 55 60

Asn Asn Gly Met Gly Asp Arg Glu Lys Pro Leu Asp Val Phe Tyr Pro

65 70 75 80

Glu Gly Asn Arg Ala Val Glu Glu Tyr Thr Glu Phe Ile Lys Arg Ala

85 90 95

Asn Pro Asp Leu Arg Phe Ser Phe Asn Val His Pro Leu Lys Glu Gly

100 105 110

Glu Arg Val Phe Leu Arg Asn Ala Gly Gly Phe Lys Arg Tyr Val Gln

115 120 125

Pro Phe Arg Thr Lys His Val Ser Ser Glu Val Ser Phe Gly Tyr His

130 135 140

Ile Phe Glu Val Arg Arg Lys Leu Lys Lys Glu Phe Gln Gly Leu Asp

145 150 155 160

Ser Lys Glu Ile Ser Arg Leu Val Lys Glu Lys Gly Arg Asp Phe Val

165 170 175

Thr Glu Glu Tyr His Lys Lys Val Leu Thr Ile Ser Gly Asp Ser Leu

180 185 190

Ala Leu Asp Pro Glu Glu Ile Arg Gly Thr Glu Leu Leu Ile His Glu

195 200 205

Cys Thr Phe Leu Asp Ala Arg Asp Arg Arg Tyr Lys Asn His Ala Ala

210 215 220

Ile Asp Glu Val Met Glu Ser Val Lys Ala Ala Gly Val Lys Lys Val

225 230 235 240

Ile Leu Tyr His Ile Ser Thr Arg Tyr Ile Arg Gln Leu Lys Ser Val

245 250 255

Ile Lys Lys Tyr Arg Glu Glu Met Pro Asp Val Glu Ile Leu Tyr Met

260 265 270

Asp Pro Arg Lys Val Phe Glu Met

275 280

<210> 53

<211> 107

<212> PRT

<213> 宏基因组(metagenomic)

<400> 53

Met Gln Asp Trp Ala Thr Phe Lys Lys Lys His Leu Thr Asp Thr Trp

1 5 10 15

Asp Val Asp Cys Asp Asn Leu Met Pro Thr Ser Leu Phe Asp Cys Lys

20 25 30

Asp Lys Asn Thr Phe Ile Tyr Ser Leu Pro Gly Pro Val Lys Ala Leu

35 40 45

Cys Arg Gly Val Ile Phe Ser Ala Asp Val Leu Ser Asn Ser Glu Phe

50 55 60

Tyr Leu Ala Glu Cys Asn Val Lys Pro Arg Lys Pro Cys Lys Tyr Lys

65 70 75 80

Leu Lys Lys Ser Ser Asn Arg Ile Cys Ile Arg Cys Glu His Glu Leu

85 90 95

Pro Val His Phe Ala Gly Val Gly Ile Cys Pro

100 105

<210> 54

<211> 106

<212> PRT

<213> 宏基因组(metagenomic)

<400> 54

Met Gln Asn Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Arg

1 5 10 15

Asp Val Lys Cys Asp Ala Glu Met Lys Lys Ala Leu Phe Asp Cys Lys

20 25 30

Gln Lys Asn Thr Phe Ile Tyr Ala Arg Pro Gly Arg Val Gln Ala Leu

35 40 45

Cys Lys Asn Ile Ile Val Ser Lys Asn Val Leu Ser Thr Asp Glu Phe

50 55 60

Tyr Leu Ser Asp Cys Asn Arg Ile Lys Leu Pro Cys His Tyr Lys Leu

65 70 75 80

Lys Lys Ser Ser Asn Thr Ile Cys Ile Thr Cys Glu Asn Lys Leu Pro

85 90 95

Val His Phe Val Ala Val Glu Glu Cys Pro

100 105

<210> 55

<211> 284

<212> PRT

<213> 宏基因组(metagenomic)

<400> 55

Met Val Leu Met Ile Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val

1 5 10 15

Ala Leu Arg Ala Leu Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu

20 25 30

Pro Val Val Leu Leu Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu

35 40 45

Ile Ser Ala Ala Val Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro

50 55 60

Glu Ile Phe Glu Gln Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro

65 70 75 80

Gln Leu Leu Phe Leu Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr

85 90 95

Ser Asp Thr Arg Arg Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu

100 105 110

Glu Ser Ala Ile Asp Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser

115 120 125

Arg Ala Asp Leu Ile Val Asp Thr Ser Glu Met Ser Val His Glu Leu

130 135 140

Ala Glu Met Leu Arg Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu

145 150 155 160

Thr Met Val Phe Glu Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp

165 170 175

Ala Asp Tyr Val Phe Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp

180 185 190

Pro Lys Leu Arg Pro Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe

195 200 205

Leu Asp Arg His Thr Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser

210 215 220

Tyr Leu Glu Leu Trp Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr

225 230 235 240

Leu Thr Val Ala Ile Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr

245 250 255

Ile Ala Glu Gln Leu Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val

260 265 270

Gln Ser Arg His Arg Thr Leu Glu Lys Arg Lys Pro

275 280

<210> 56

<211> 295

<212> PRT

<213> 宏基因组(metagenomic)

<400> 56

Met Trp Ser His Pro Gln Phe Glu Lys Ala Ser Met Val Leu Met Ile

1 5 10 15

Val Ser Gly Arg Ser Gly Ser Gly Lys Ser Val Ala Leu Arg Ala Leu

20 25 30

Glu Asp Met Gly Phe Tyr Cys Val Asp Asn Leu Pro Val Val Leu Leu

35 40 45

Pro Asp Leu Ala Arg Thr Leu Ala Asp Arg Glu Ile Ser Ala Ala Val

50 55 60

Ser Ile Asp Val Arg Asn Met Pro Glu Ser Pro Glu Ile Phe Glu Gln

65 70 75 80

Ala Met Ser Asn Leu Pro Asp Ala Phe Ser Pro Gln Leu Leu Phe Leu

85 90 95

Asp Ala Asp Arg Asn Thr Leu Ile Arg Arg Tyr Ser Asp Thr Arg Arg

100 105 110

Leu His Pro Leu Ser Ser Lys Asn Leu Ser Leu Glu Ser Ala Ile Asp

115 120 125

Lys Glu Ser Asp Leu Leu Glu Pro Leu Arg Ser Arg Ala Asp Leu Ile

130 135 140

Val Asp Thr Ser Glu Met Ser Val His Glu Leu Ala Glu Met Leu Arg

145 150 155 160

Thr Arg Leu Leu Gly Lys Arg Glu Arg Glu Leu Thr Met Val Phe Glu

165 170 175

Ser Phe Gly Phe Lys His Gly Ile Pro Ile Asp Ala Asp Tyr Val Phe

180 185 190

Asp Val Arg Phe Leu Pro Asn Pro His Trp Asp Pro Lys Leu Arg Pro

195 200 205

Met Thr Gly Leu Asp Lys Pro Val Ala Ala Phe Leu Asp Arg His Thr

210 215 220

Glu Val His Asn Phe Ile Tyr Gln Thr Arg Ser Tyr Leu Glu Leu Trp

225 230 235 240

Leu Pro Met Leu Glu Thr Asn Asn Arg Ser Tyr Leu Thr Val Ala Ile

245 250 255

Gly Cys Thr Gly Gly Lys His Arg Ser Val Tyr Ile Ala Glu Gln Leu

260 265 270

Ala Asp Tyr Phe Arg Ser Arg Gly Lys Asn Val Gln Ser Arg His Arg

275 280 285

Thr Leu Glu Lys Arg Lys Pro

290 295

<210> 57

<211> 150

<212> PRT

<213> 宏基因组(metagenomic)

<400> 57

Met Tyr Gln Leu Glu Lys Glu Pro Ile Val Gly Ala Glu Thr Phe Tyr

1 5 10 15

Val Asp Gly Ala Ala Asn Arg Glu Thr Lys Leu Gly Lys Ala Gly Tyr

20 25 30

Val Thr Asn Arg Gly Arg Gln Lys Val Val Thr Leu Thr Asp Thr Thr

35 40 45

Asn Gln Lys Thr Glu Leu Gln Ala Ile Tyr Leu Ala Leu Gln Asp Ser

50 55 60

Gly Leu Glu Val Asn Ile Val Thr Asp Ser Gln Tyr Ala Leu Gly Ile

65 70 75 80

Ile Thr Gln Trp Ile His Asn Trp Lys Lys Arg Gly Trp Lys Thr Ala

85 90 95

Asp Lys Lys Pro Val Lys Asn Val Asp Leu Val Asn Gln Ile Ile Glu

100 105 110

Gln Leu Ile Lys Lys Glu Lys Val Tyr Leu Ala Trp Val Pro Ala His

115 120 125

Lys Gly Ile Gly Gly Asn Glu Gln Val Asp Lys Leu Val Ser Ala Gly

130 135 140

Ile Arg Lys Val Leu Phe

145 150

<210> 58

<211> 157

<212> PRT

<213> 宏基因组(metagenomic)

<400> 58

Pro Asp Leu Thr Asp Gln Pro Ile Pro Asp Ala Asp Tyr Thr Trp Tyr

1 5 10 15

Thr Asp Gly Ser Ser Phe Leu Gln Glu Gly Gln Arg Arg Ala Gly Ala

20 25 30

Ala Val Thr Thr Glu Thr Glu Val Ile Trp Ala Arg Ala Leu Pro Ala

35 40 45

Gly Thr Ser Ala Gln Arg Ala Glu Leu Ile Ala Leu Thr Gln Ala Leu

50 55 60

Lys Met Ala Glu Gly Lys Lys Leu Asn Val Tyr Thr Asp Ser Arg Tyr

65 70 75 80

Ala Phe Ala Thr Ala His Val His Gly Glu Ile Tyr Arg Arg Arg Gly

85 90 95

Leu Leu Thr Ser Glu Gly Arg Glu Ile Lys Asn Lys Asn Glu Ile Leu

100 105 110

Ala Leu Leu Lys Ala Leu Phe Leu Pro Lys Arg Leu Ser Ile Ile His

115 120 125

Cys Pro Gly His Gln Lys Gly Asn Ser Ala Glu Ala Arg Gly Asn Arg

130 135 140

Met Ala Asp Gln Ala Ala Arg Glu Ala Ala Met Lys Ala

145 150 155

<210> 59

<211> 440

<212> PRT

<213> 宏基因组(metagenomic)

<400> 59

Pro Ile Ser Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met

1 5 10 15

Asp Gly Pro Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys

20 25 30

Ala Leu Val Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser

35 40 45

Lys Ile Gly Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys

50 55 60

Lys Lys Asp Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu

65 70 75 80

Asn Lys Arg Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His

85 90 95

Pro Ala Gly Leu Lys Lys Lys Lys Ser Val Thr Val Leu Asp Val Gly

100 105 110

Asp Ala Tyr Phe Ser Val Pro Leu Asp Glu Asp Phe Arg Lys Tyr Thr

115 120 125

Ala Phe Thr Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr

130 135 140

Gln Tyr Asn Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe

145 150 155 160

Gln Ser Ser Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro

165 170 175

Asp Ile Val Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp

180 185 190

Leu Glu Ile Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln His

195 200 205

Leu Leu Arg Trp Gly Leu Thr Thr Pro Asp Lys Lys His Gln Lys Glu

210 215 220

Pro Pro Phe Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr

225 230 235 240

Val Gln Pro Ile Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp

245 250 255

Ile Gln Lys Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Pro

260 265 270

Gly Ile Lys Val Arg Gln Leu Ser Lys Leu Leu Arg Gly Thr Lys Ala

275 280 285

Leu Thr Glu Val Ile Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala

290 295 300

Glu Asn Arg Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp

305 310 315 320

Pro Ser Lys Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln

325 330 335

Trp Thr Tyr Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly

340 345 350

Lys Tyr Ala Arg Met Arg Gly Ala His Thr Asn Asp Val Lys Gln Leu

355 360 365

Thr Glu Ala Val Gln Lys Ile Thr Thr Glu Ser Ile Val Ile Trp Gly

370 375 380

Lys Thr Pro Lys Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Thr

385 390 395 400

Trp Trp Thr Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe

405 410 415

Val Asn Thr Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu

420 425 430

Pro Ile Val Gly Ala Glu Thr Phe

435 440

<210> 60

<211> 105

<212> PRT

<213> 宏基因组(metagenomic)

<400> 60

Gln Asp Trp Glu Thr Phe Gln Lys Lys His Leu Thr Asp Thr Lys Lys

1 5 10 15

Val Lys Cys Asp Val Glu Met Ala Lys Ala Leu Phe Asp Cys Lys Lys

20 25 30

Thr Asn Thr Phe Ile Tyr Ala Leu Pro Gly Arg Val Lys Ala Leu Cys

35 40 45

Lys Asn Ile Arg Asp Asn Thr Asp Val Leu Ser Arg Asp Ala Phe Leu

50 55 60

Leu Pro Gln Cys Asp Arg Ile Lys Leu Pro Cys His Tyr Lys Leu Ser

65 70 75 80

Ser Ser Thr Asn Thr Ile Cys Ile Thr Cys Val Asn Gln Leu Pro Ile

85 90 95

His Phe Ala Gly Val Gly Ser Cys Pro

100 105

<210> 61

<211> 28

<212> PRT

<213> 宏基因组(metagenomic)

<400> 61

Gln Ser Pro Met Pro Leu Thr Val Ala Ala Ala Ser Pro Glu Leu Ala

1 5 10 15

Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile Val Arg

20 25

<210> 62

<211> 531

<212> PRT

<213> 宏基因组(metagenomic)

<400> 62

Ser Asn Ala Met Ala Ala Ser Asp Gln His Arg Ser Arg Arg His Asp

1 5 10 15

Glu Ser Ser Ser Arg Pro Asn Lys Lys Lys Lys Val Ser Arg Asn Pro

20 25 30

Glu Thr Asn Leu Leu Phe Asn Leu Asn Ser Cys Ser Lys Ser Lys Asp

35 40 45

Leu Ser Ala Ala Leu Ala Leu Tyr Asp Ala Ala Ile Thr Ser Ser Glu

50 55 60

Val Arg Leu Ser Gln Gln His Phe Gln Thr Leu Leu Tyr Leu Cys Ser

65 70 75 80

Ala Ser Ile Thr Asp Ile Ser Leu Gln Tyr Leu Ala Ile Asp Arg Gly

85 90 95

Phe Glu Ile Phe Asp Arg Met Val Ser Ser Gly Ile Ser Pro Asn Glu

100 105 110

Ala Ser Val Thr Ser Val Ala Arg Leu Ala Ala Ala Lys Gly Asn Gly

115 120 125

Asp Tyr Ala Phe Lys Val Val Lys Glu Phe Val Ser Val Gly Gly Val

130 135 140

Ser Ile Pro Arg Leu Arg Thr Tyr Ala Pro Ala Leu Leu Cys Phe Cys

145 150 155 160

Glu Lys Leu Glu Ala Glu Lys Gly Tyr Glu Val Glu Glu His Met Glu

165 170 175

Ala Ala Gly Ile Ala Leu Glu Glu Ala Glu Ile Ser Ala Leu Leu Lys

180 185 190

Val Ser Ala Ala Thr Gly Arg Glu Asn Lys Val Tyr Arg Tyr Leu His

195 200 205

Lys Leu Arg Glu Tyr Val Gly Cys Val Ser Glu Glu Thr Leu Lys Ile

210 215 220

Ile Glu Glu Trp Phe Cys Gly Glu Lys Ala Gly Glu Val Gly Asp Asn

225 230 235 240

Gly Ile Gly Ser Asp Val Gly Met Leu Arg Glu Ala Val Leu Asn Asn

245 250 255

Gly Gly Gly Trp His Gly His Gly Trp Val Gly Glu Gly Lys Trp Thr

260 265 270

Val Lys Lys Gly Asn Val Ser Ser Thr Gly Arg Cys Leu Ser Cys Ser

275 280 285

Glu Gln Leu Ala Cys Val Asp Thr Asn Glu Val Glu Thr Gln Lys Phe

290 295 300

Val Asp Ser Leu Val Ala Leu Ala Met Asp Arg Lys Thr Lys Met Asn

305 310 315 320

Ser Cys Glu Thr Asn Val Val Phe Ser Glu Phe Gln Asp Trp Leu Glu

325 330 335

Lys His Gly Asp Tyr Glu Ala Ile Val Asp Gly Ala Asn Ile Gly Leu

340 345 350

Tyr Gln Gln Asn Phe Val Asp Gly Ser Phe Ser Leu Ser Gln Leu Glu

355 360 365

Ser Val Met Lys Glu Leu Tyr Arg Glu Ser Gly Asn Asn Lys Trp Pro

370 375 380

Leu Ile Leu Leu His Lys Arg Arg Val Lys Thr Leu Leu Glu Asn Pro

385 390 395 400

Thr His Arg Asn Leu Val Glu Glu Trp Ile Ser Asn Gly Val Leu Tyr

405 410 415

Ala Thr Pro Pro Gly Ser Asn Asp Asp Trp Tyr Trp Leu Tyr Ala Ala

420 425 430

Ala Lys Leu Lys Cys Leu Leu Val Thr Asn Asp Glu Met Arg Asp His

435 440 445

Ile Phe Glu Leu Leu Gly Ser Thr Phe Phe Gln Lys Trp Lys Glu Arg

450 455 460

His Gln Val Arg Tyr Thr Phe Val Lys Gly Asn Leu Lys Leu Glu Met

465 470 475 480

Pro Ser Pro Phe Ser Val Val Ile Gln Glu Ser Glu Lys Gly Ser Trp

485 490 495

His Phe Pro Val Ser Cys Glu Asn Asn Glu Glu Ser Ser Arg Thr Trp

500 505 510

Met Cys Ile Ser Arg Gln Ser Ile Leu Asp Ser Pro Lys Ser Asn Gly

515 520 525

Lys Ile Pro

530

<210> 63

<211> 208

<212> PRT

<213> 宏基因组(metagenomic)

<400> 63

Ser Ser Arg Lys Ala Lys Lys Lys Ala Ile Gln Gln Ser Pro Glu Ala

1 5 10 15

Leu Leu Lys Gln Lys Leu Asp Met Cys Ser Lys Lys Gly Asp Val Leu

20 25 30

Glu Ala Leu Arg Leu Tyr Asp Glu Ala Arg Arg Asn Gly Val Gln Leu

35 40 45

Ser Gln Tyr His Tyr Asn Val Leu Leu Tyr Val Cys Ser Leu Ala Ser

50 55 60

Ala Ser Ser Asn Pro Gly Leu Ser Arg Gly Phe Asp Ile Phe Lys Gln

65 70 75 80

Met Ile Val Asp Lys Val Val Pro Asn Glu Ala Thr Phe Thr Asn Gly

85 90 95

Ala Arg Leu Ala Val Ala Lys Asp Asp Pro Glu Met Ala Phe Asp Met

100 105 110

Val Lys Gln Met Lys Ala Phe Gly Ile Gln Pro Arg Leu Arg Ser Tyr

115 120 125

Gly Pro Ala Leu Phe Gly Phe Cys Arg Lys Gly Asp Ala Asp Lys Ala

130 135 140

Tyr Glu Val Asp Ala His Met Val Glu Ser Glu Val Val Pro Glu Glu

145 150 155 160

Pro Glu Leu Ala Ala Leu Leu Lys Val Ser Met Asp Thr Lys Asn Ala

165 170 175

Asp Lys Val Asn Lys Thr Leu Gln Arg Leu Arg Asp Leu Val Arg Gln

180 185 190

Val Ser Lys Ser Thr Gln Asp Met Ile Glu Glu Trp Gln Lys Ser Glu

195 200 205

<210> 64

<211> 6

<212> PRT

<213> 宏基因组(metagenomic)

<400> 64

Ser Ser Thr Ser Ala Ala

1 5

<210> 65

<211> 200

<212> PRT

<213> 宏基因组(metagenomic)

<400> 65

Tyr Asp Tyr Phe Gln Phe Thr Gln Gln Tyr Gln Leu Ala Val Cys Asn

1 5 10 15

Ser Asn Arg Thr Leu Cys Lys Asp Pro Pro Asp Lys Leu Phe Thr Val

20 25 30

His Gly Leu Trp Pro Ser Asn Met Val Gly Pro Asp Pro Ser Lys Cys

35 40 45

Pro Ile Lys Asn Ile Arg Lys Arg Glu Lys Leu Leu Glu His Gln Leu

50 55 60

Glu Ile Ile Trp Pro Asn Val Phe Asp Arg Thr Lys Asn Asn Leu Phe

65 70 75 80

Trp Asp Lys Glu Trp Met Lys His Gly Ser Cys Gly Tyr Pro Thr Ile

85 90 95

Asp Asn Glu Asn His Tyr Phe Glu Thr Val Ile Lys Met Tyr Ile Ser

100 105 110

Lys Lys Gln Asn Val Ser Arg Ile Leu Ser Lys Ala Lys Ile Glu Pro

115 120 125

Asp Gly Lys Lys Arg Ala Leu Leu Asp Ile Glu Asn Ala Ile Arg Asn

130 135 140

Gly Ala Asp Asn Lys Lys Pro Lys Leu Lys Cys Gln Lys Lys Gly Thr

145 150 155 160

Thr Thr Glu Leu Val Glu Ile Thr Leu Cys Ser Asp Lys Ser Gly Glu

165 170 175

His Phe Ile Asp Cys Pro His Pro Phe Glu Pro Ile Ser Pro His Tyr

180 185 190

Cys Pro Thr Asn Asn Ile Lys Tyr

195 200

<210> 66

<211> 217

<212> PRT

<213> 宏基因组(metagenomic)

<400> 66

Tyr Val Glu Phe Ala Gln Asp Phe Asp Phe Phe Tyr Phe Val Gln Gln

1 5 10 15

Trp Pro Ala Ser Tyr Cys Asp Thr Arg Arg Ser Cys Cys Tyr Pro Thr

20 25 30

Thr Gly Lys Pro Asp Glu Asp Phe Ser Ile His Gly Leu Trp Pro Asn

35 40 45

Tyr Glu Asn Gly Lys Trp Pro Gln Asn Cys Asp Arg Glu Ser Ser Leu

50 55 60

Asp Glu Ser Glu Ile Ser Asp Leu Ile Ser Thr Met Glu Lys Asn Trp

65 70 75 80

Pro Ser Leu Ala Cys Pro Ser Ser Asp Gly Val Arg Phe Trp Ser His

85 90 95

Glu Trp Leu Lys His Gly Thr Cys Ser Ala Leu Gly Glu Arg Ala Tyr

100 105 110

Phe Gln Ala Ala Leu Asp Phe Arg Lys Lys Ser Asn Leu Leu Glu Asn

115 120 125

Leu Lys Asn Ala Glu Ile Thr Pro Arg Asn Gly Glu His Tyr Thr Leu

130 135 140

Glu Ser Ile Lys Lys Ala Ile Glu Glu Gly Val Gly His Ser Pro Tyr

145 150 155 160

Ile Glu Cys Asn Val Asp Thr Gln Gly Asn His Gln Ile Tyr Gln Val

165 170 175

Tyr Leu Cys Val Asp Lys Thr Ala Thr Asp Phe Ile Asp Cys Pro Ile

180 185 190

Phe Pro His Gly Arg Gly Cys Gly Ser Lys Ile Glu Phe Pro Pro Phe

195 200 205

Ser Ser Glu Ser Asp His Asp Glu Phe

210 215

<210> 67

<211> 1440

<212> PRT

<213> 宏基因组(metagenomic)

<400> 67

Ser Asn Ala Met Lys Ile Ser Lys Val Arg Glu Glu Asn Arg Gly Ala

1 5 10 15

Lys Leu Thr Val Asn Ala Lys Thr Ala Val Val Ser Glu Asn Arg Ser

20 25 30

Gln Glu Gly Ile Leu Tyr Asn Asp Pro Ser Arg Tyr Gly Lys Ser Arg

35 40 45

Lys Asn Asp Glu Asp Arg Asp Arg Tyr Ile Glu Ser Arg Leu Lys Ser

50 55 60

Ser Gly Lys Leu Tyr Arg Ile Phe Asn Glu Asp Lys Asn Lys Arg Glu

65 70 75 80

Thr Asp Glu Leu Gln Trp Phe Leu Ser Glu Ile Val Lys Lys Ile Asn

85 90 95

Arg Arg Asn Gly Leu Val Leu Ser Asp Met Leu Ser Val Asp Asp Arg

100 105 110

Ala Phe Glu Lys Ala Phe Glu Lys Tyr Ala Glu Leu Ser Tyr Thr Asn

115 120 125

Arg Arg Asn Lys Val Ser Gly Ser Pro Ala Phe Glu Thr Cys Gly Val

130 135 140

Asp Ala Ala Thr Ala Glu Arg Leu Lys Gly Ile Ile Ser Glu Thr Asn

145 150 155 160

Phe Ile Asn Arg Ile Lys Asn Asn Ile Asp Asn Lys Val Ser Glu Asp

165 170 175

Ile Ile Asp Arg Ile Ile Ala Lys Tyr Leu Lys Lys Ser Leu Cys Arg

180 185 190

Glu Arg Val Lys Arg Gly Leu Lys Lys Leu Leu Met Asn Ala Phe Asp

195 200 205

Leu Pro Tyr Ser Asp Pro Asp Ile Asp Val Gln Arg Asp Phe Ile Asp

210 215 220

Tyr Val Leu Glu Asp Phe Tyr His Val Arg Ala Lys Ser Gln Val Ser

225 230 235 240

Arg Ser Ile Lys Asn Met Asn Met Pro Val Gln Pro Glu Gly Asp Gly

245 250 255

Lys Phe Ala Ile Thr Val Ser Lys Gly Gly Thr Glu Ser Gly Asn Lys

260 265 270

Arg Ser Ala Glu Lys Glu Ala Phe Lys Lys Phe Leu Ser Asp Tyr Ala

275 280 285

Ser Leu Asp Glu Arg Val Arg Asp Asp Met Leu Arg Arg Met Arg Arg

290 295 300

Leu Val Val Leu Tyr Phe Tyr Gly Ser Asp Asp Ser Lys Leu Ser Asp

305 310 315 320

Val Asn Glu Lys Phe Asp Val Trp Glu Asp His Ala Ala Arg Arg Val

325 330 335

Asp Asn Arg Glu Phe Ile Lys Leu Pro Leu Glu Asn Lys Leu Ala Asn

340 345 350

Gly Lys Thr Asp Lys Asp Ala Glu Arg Ile Arg Lys Asn Thr Val Lys

355 360 365

Glu Leu Tyr Arg Asn Gln Asn Ile Gly Cys Tyr Arg Gln Ala Val Lys

370 375 380

Ala Val Glu Glu Asp Asn Asn Gly Arg Tyr Phe Asp Asp Lys Met Leu

385 390 395 400

Asn Met Phe Phe Ile His Arg Ile Glu Tyr Gly Val Glu Lys Ile Tyr

405 410 415

Ala Asn Leu Lys Gln Val Thr Glu Phe Lys Ala Arg Thr Gly Tyr Leu

420 425 430

Ser Glu Lys Ile Trp Lys Asp Leu Ile Asn Tyr Ile Ser Ile Lys Tyr

435 440 445

Ile Ala Met Gly Lys Ala Val Tyr Asn Tyr Ala Met Asp Glu Leu Asn

450 455 460

Ala Ser Asp Lys Lys Glu Ile Glu Leu Gly Lys Ile Ser Glu Glu Tyr

465 470 475 480

Leu Ser Gly Ile Ser Ser Phe Asp Tyr Glu Leu Ile Lys Ala Glu Glu

485 490 495

Met Leu Gln Arg Glu Thr Ala Val Tyr Val Ala Phe Ala Ala Arg His

500 505 510

Leu Ser Ser Gln Thr Val Glu Leu Asp Ser Glu Asn Ser Asp Phe Leu

515 520 525

Leu Leu Lys Pro Lys Gly Thr Met Asp Lys Asn Asp Lys Asn Lys Leu

530 535 540

Ala Ser Asn Asn Ile Leu Asn Phe Leu Lys Asp Lys Glu Thr Leu Arg

545 550 555 560

Asp Thr Ile Leu Gln Tyr Phe Gly Gly His Ser Leu Trp Thr Asp Phe

565 570 575

Pro Phe Asp Lys Tyr Leu Ala Gly Gly Lys Asp Asp Val Asp Phe Leu

580 585 590

Thr Asp Leu Lys Asp Val Ile Tyr Ser Met Arg Asn Asp Ser Phe His

595 600 605

Tyr Ala Thr Glu Asn His Asn Asn Gly Lys Trp Asn Lys Glu Leu Ile

610 615 620

Ser Ala Met Phe Glu His Glu Thr Glu Arg Met Thr Val Val Met Lys

625 630 635 640

Asp Lys Phe Tyr Ser Asn Asn Leu Pro Met Phe Tyr Lys Asn Asp Asp

645 650 655

Leu Lys Lys Leu Leu Ile Asp Leu Tyr Lys Asp Asn Val Glu Arg Ala

660 665 670

Ser Gln Val Pro Ser Phe Asn Lys Val Phe Val Arg Lys Asn Phe Pro

675 680 685

Ala Leu Val Arg Asp Lys Asp Asn Leu Gly Ile Glu Leu Asp Leu Lys

690 695 700

Ala Asp Ala Asp Lys Gly Glu Asn Glu Leu Lys Phe Tyr Asn Ala Leu

705 710 715 720

Tyr Tyr Met Phe Lys Glu Ile Tyr Tyr Asn Ala Phe Leu Asn Asp Lys

725 730 735

Asn Val Arg Glu Arg Phe Ile Thr Lys Ala Thr Lys Val Ala Asp Asn

740 745 750

Tyr Asp Arg Asn Lys Glu Arg Asn Leu Lys Asp Arg Ile Lys Ser Ala

755 760 765

Gly Ser Asp Glu Lys Lys Lys Leu Arg Glu Gln Leu Gln Asn Tyr Ile

770 775 780

Ala Glu Asn Asp Phe Gly Gln Arg Ile Lys Asn Ile Val Gln Val Asn

785 790 795 800

Pro Asp Tyr Thr Leu Ala Gln Ile Cys Gln Leu Ile Met Thr Glu Tyr

805 810 815

Asn Gln Gln Asn Asn Gly Cys Met Gln Lys Lys Ser Ala Ala Arg Lys

820 825 830

Asp Ile Asn Lys Asp Ser Tyr Gln His Tyr Lys Met Leu Leu Leu Val

835 840 845

Asn Leu Arg Lys Ala Phe Leu Glu Phe Ile Lys Glu Asn Tyr Ala Phe

850 855 860

Val Leu Lys Pro Tyr Lys His Asp Leu Cys Asp Lys Ala Asp Phe Val

865 870 875 880

Pro Asp Phe Ala Lys Tyr Val Lys Pro Tyr Ala Gly Leu Ile Ser Arg

885 890 895

Val Ala Gly Ser Ser Glu Leu Gln Lys Trp Tyr Ile Val Ser Arg Phe

900 905 910

Leu Ser Pro Ala Gln Ala Asn His Met Leu Gly Phe Leu His Ser Tyr

915 920 925

Lys Gln Tyr Val Trp Asp Ile Tyr Arg Arg Ala Ser Glu Thr Gly Thr

930 935 940

Glu Ile Asn His Ser Ile Ala Glu Asp Lys Ile Ala Gly Val Asp Ile

945 950 955 960

Thr Asp Val Asp Ala Val Ile Asp Leu Ser Val Lys Leu Cys Gly Thr

965 970 975

Ile Ser Ser Glu Ile Ser Asp Tyr Phe Lys Asp Asp Glu Val Tyr Ala

980 985 990

Glu Tyr Ile Ser Ser Tyr Leu Asp Phe Glu Tyr Asp Gly Gly Asn Tyr

995 1000 1005

Lys Asp Ser Leu Asn Arg Phe Cys Asn Ser Asp Ala Val Asn Asp Gln

1010 1015 1020

Lys Val Ala Leu Tyr Tyr Asp Gly Glu His Pro Lys Leu Asn Arg Asn

1025 1030 1035 1040

Ile Ile Leu Ser Lys Leu Tyr Gly Glu Arg Arg Phe Leu Glu Lys Ile

1045 1050 1055

Thr Asp Arg Val Ser Arg Ser Asp Ile Val Glu Tyr Tyr Lys Leu Lys

1060 1065 1070

Lys Glu Thr Ser Gln Tyr Gln Thr Lys Gly Ile Phe Asp Ser Glu Asp

1075 1080 1085

Glu Gln Lys Asn Ile Lys Lys Phe Gln Glu Met Lys Asn Ile Val Glu

1090 1095 1100

Phe Arg Asp Leu Met Asp Tyr Ser Glu Ile Ala Asp Glu Leu Gln Gly

1105 1110 1115 1120

Gln Leu Ile Asn Trp Ile Tyr Leu Arg Glu Arg Asp Leu Met Asn Phe

1125 1130 1135

Gln Leu Gly Tyr His Tyr Ala Cys Leu Asn Asn Asp Ser Asn Lys Gln

1140 1145 1150

Ala Thr Tyr Val Thr Leu Asp Tyr Gln Gly Lys Lys Asn Arg Lys Ile

1155 1160 1165

Asn Gly Ala Ile Leu Tyr Gln Ile Cys Ala Met Tyr Ile Asn Gly Leu

1170 1175 1180

Pro Leu Tyr Tyr Val Asp Lys Asp Ser Ser Glu Trp Thr Val Ser Asp

1185 1190 1195 1200

Gly Lys Glu Ser Thr Gly Ala Lys Ile Gly Glu Phe Tyr Arg Tyr Ala

1205 1210 1215

Lys Ser Phe Glu Asn Thr Ser Asp Cys Tyr Ala Ser Gly Leu Glu Ile

1220 1225 1230

Phe Glu Asn Ile Ser Glu His Asp Asn Ile Thr Glu Leu Arg Asn Tyr

1235 1240 1245

Ile Glu His Phe Arg Tyr Tyr Ser Ser Phe Asp Arg Ser Phe Leu Gly

1250 1255 1260

Ile Tyr Ser Glu Val Phe Asp Arg Phe Phe Thr Tyr Asp Leu Lys Tyr

1265 1270 1275 1280

Arg Lys Asn Val Pro Thr Ile Leu Tyr Asn Ile Leu Leu Gln His Phe

1285 1290 1295

Val Asn Val Arg Phe Glu Phe Val Ser Gly Lys Lys Met Ile Gly Ile

1300 1305 1310

Asp Lys Lys Asp Arg Lys Ile Ala Lys Glu Lys Glu Cys Ala Arg Ile

1315 1320 1325

Thr Ile Arg Glu Lys Asn Gly Val Tyr Ser Glu Gln Phe Thr Tyr Lys

1330 1335 1340

Leu Lys Asn Gly Thr Val Tyr Val Asp Ala Arg Asp Lys Arg Tyr Leu

1345 1350 1355 1360

Gln Ser Ile Ile Arg Leu Leu Phe Tyr Pro Glu Lys Val Asn Met Asp

1365 1370 1375

Glu Met Ile Glu Val Lys Glu Lys Lys Lys Pro Ser Asp Asn Asn Thr

1380 1385 1390

Gly Lys Gly Tyr Ser Lys Arg Asp Arg Gln Gln Asp Arg Lys Glu Tyr

1395 1400 1405

Asp Lys Tyr Lys Glu Lys Lys Lys Lys Glu Gly Asn Phe Leu Ser Gly

1410 1415 1420

Met Gly Gly Asn Ile Asn Trp Asp Glu Ile Asn Ala Gln Leu Lys Asn

1425 1430 1435 1440

<210> 68

<211> 1159

<212> PRT

<213> 宏基因组(metagenomic)

<400> 68

Met Lys Val Thr Lys Val Gly Gly Ile Ser His Lys Lys Tyr Thr Ser

1 5 10 15

Glu Gly Arg Leu Val Lys Ser Glu Ser Glu Glu Asn Arg Thr Asp Glu

20 25 30

Arg Leu Ser Ala Leu Leu Asn Met Arg Leu Asp Met Tyr Ile Lys Asn

35 40 45

Pro Ser Ser Thr Glu Thr Lys Glu Asn Gln Lys Arg Ile Gly Lys Leu

50 55 60

Lys Lys Phe Phe Ser Asn Lys Met Val Tyr Leu Lys Asp Asn Thr Leu

65 70 75 80

Ser Leu Lys Asn Gly Lys Lys Glu Asn Ile Asp Arg Glu Tyr Ser Glu

85 90 95

Thr Asp Ile Leu Glu Ser Asp Val Arg Asp Lys Lys Asn Phe Ala Val

100 105 110

Leu Lys Lys Ile Tyr Leu Asn Glu Asn Val Asn Ser Glu Glu Leu Glu

115 120 125

Val Phe Arg Asn Asp Ile Lys Lys Lys Leu Asn Lys Ile Asn Ser Leu

130 135 140

Lys Tyr Ser Phe Glu Lys Asn Lys Ala Asn Tyr Gln Lys Ile Asn Glu

145 150 155 160

Asn Asn Ile Glu Lys Val Glu Gly Lys Ser Lys Arg Asn Ile Ile Tyr

165 170 175

Asp Tyr Tyr Arg Glu Ser Ala Lys Arg Asp Ala Tyr Val Ser Asn Val

180 185 190

Lys Glu Ala Phe Asp Lys Leu Tyr Lys Glu Glu Asp Ile Ala Lys Leu

195 200 205

Val Leu Glu Ile Glu Asn Leu Thr Lys Leu Glu Lys Tyr Lys Ile Arg

210 215 220

Glu Phe Tyr His Glu Ile Ile Gly Arg Lys Asn Asp Lys Glu Asn Phe

225 230 235 240

Ala Lys Ile Ile Tyr Glu Glu Ile Gln Asn Val Asn Asn Met Lys Glu

245 250 255

Leu Ile Glu Lys Val Pro Asp Met Ser Glu Leu Lys Lys Ser Gln Val

260 265 270

Phe Tyr Lys Tyr Tyr Leu Asp Lys Glu Glu Leu Asn Asp Lys Asn Ile

275 280 285

Lys Tyr Ala Phe Cys His Phe Val Glu Ile Glu Met Ser Gln Leu Leu

290 295 300

Lys Asn Tyr Val Tyr Lys Arg Leu Ser Asn Ile Ser Asn Asp Lys Ile

305 310 315 320

Lys Arg Ile Phe Glu Tyr Gln Asn Leu Lys Lys Leu Ile Glu Asn Lys

325 330 335

Leu Leu Asn Lys Leu Asp Thr Tyr Val Arg Asn Cys Gly Lys Tyr Asn

340 345 350

Tyr Tyr Leu Gln Asp Gly Glu Ile Ala Thr Ser Asp Phe Ile Ala Arg

355 360 365

Asn Arg Gln Asn Glu Ala Phe Leu Arg Asn Ile Ile Gly Val Ser Ser

370 375 380

Val Ala Tyr Phe Ser Leu Arg Asn Ile Leu Glu Thr Glu Asn Glu Asn

385 390 395 400

Asp Ile Thr Gly Arg Met Arg Gly Lys Thr Val Lys Asn Asn Lys Gly

405 410 415

Glu Glu Lys Tyr Val Ser Gly Glu Val Asp Lys Ile Tyr Asn Glu Asn

420 425 430

Lys Lys Asn Glu Val Lys Glu Asn Leu Lys Met Phe Tyr Ser Tyr Asp

435 440 445

Phe Asn Met Asp Asn Lys Asn Glu Ile Glu Asp Phe Phe Ala Asn Ile

450 455 460

Asp Glu Ala Ile Ser Ser Ile Arg His Gly Ile Val His Phe Asn Leu

465 470 475 480

Glu Leu Glu Gly Lys Asp Ile Phe Ala Phe Lys Asn Ile Ala Pro Ser

485 490 495

Glu Ile Ser Lys Lys Met Phe Gln Asn Glu Ile Asn Glu Lys Lys Leu

500 505 510

Lys Leu Lys Ile Phe Arg Gln Leu Asn Ser Ala Asn Val Phe Arg Tyr

515 520 525

Leu Glu Lys Tyr Lys Ile Leu Asn Tyr Leu Lys Arg Thr Arg Phe Glu

530 535 540

Phe Val Asn Lys Asn Ile Pro Phe Val Pro Ser Phe Thr Lys Leu Tyr

545 550 555 560

Ser Arg Ile Asp Asp Leu Lys Asn Ser Leu Gly Ile Tyr Trp Lys Thr

565 570 575

Pro Lys Thr Asn Asp Asp Asn Lys Thr Lys Glu Ile Ile Asp Ala Gln

580 585 590

Ile Tyr Leu Leu Lys Asn Ile Tyr Tyr Gly Glu Phe Leu Asn Tyr Phe

595 600 605

Met Ser Asn Asn Gly Asn Phe Phe Glu Ile Ser Lys Glu Ile Ile Glu

610 615 620

Leu Asn Lys Asn Asp Lys Arg Asn Leu Lys Thr Gly Phe Tyr Lys Leu

625 630 635 640

Gln Lys Phe Glu Asp Ile Gln Glu Lys Ile Pro Lys Glu Tyr Leu Ala

645 650 655

Asn Ile Gln Ser Leu Tyr Met Ile Asn Ala Gly Asn Gln Asp Glu Glu

660 665 670

Glu Lys Asp Thr Tyr Ile Asp Phe Ile Gln Lys Ile Phe Leu Lys Gly

675 680 685

Phe Met Thr Tyr Leu Ala Asn Asn Gly Arg Leu Ser Leu Ile Tyr Ile

690 695 700

Gly Ser Asp Glu Glu Thr Asn Thr Ser Leu Ala Glu Lys Lys Gln Glu

705 710 715 720

Phe Asp Lys Phe Leu Lys Lys Tyr Glu Gln Asn Asn Asn Ile Lys Ile

725 730 735

Pro Tyr Glu Ile Asn Glu Phe Leu Arg Glu Ile Lys Leu Gly Asn Ile

740 745 750

Leu Lys Tyr Thr Glu Arg Leu Asn Met Phe Tyr Leu Ile Leu Lys Leu

755 760 765

Leu Asn His Lys Glu Leu Thr Asn Leu Lys Gly Ser Leu Glu Lys Tyr

770 775 780

Gln Ser Ala Asn Lys Glu Glu Ala Phe Ser Asp Gln Leu Glu Leu Ile

785 790 795 800

Asn Leu Leu Asn Leu Asp Asn Asn Arg Val Thr Glu Asp Phe Glu Leu

805 810 815

Glu Ala Asp Glu Ile Gly Lys Phe Leu Asp Phe Asn Gly Asn Lys Val

820 825 830

Lys Asp Asn Lys Glu Leu Lys Lys Phe Asp Thr Asn Lys Ile Tyr Phe

835 840 845

Asp Gly Glu Asn Ile Ile Lys His Arg Ala Phe Tyr Asn Ile Lys Lys

850 855 860

Tyr Gly Met Leu Asn Leu Leu Glu Lys Ile Ala Asp Lys Ala Gly Tyr

865 870 875 880

Lys Ile Ser Ile Glu Glu Leu Lys Lys Tyr Ser Asn Lys Lys Asn Glu

885 890 895

Ile Glu Lys Asn His Lys Met Gln Glu Asn Leu His Arg Lys Tyr Ala

900 905 910

Arg Pro Arg Lys Asp Glu Lys Phe Thr Asp Glu Asp Tyr Glu Ser Tyr

915 920 925

Lys Gln Ala Ile Glu Asn Ile Glu Glu Tyr Thr His Leu Lys Asn Lys

930 935 940

Val Glu Phe Asn Glu Leu Asn Leu Leu Gln Gly Leu Leu Leu Arg Ile

945 950 955 960

Leu His Arg Leu Val Gly Tyr Thr Ser Ile Trp Glu Arg Asp Leu Arg

965 970 975

Phe Arg Leu Lys Gly Glu Phe Pro Glu Asn Gln Tyr Ile Glu Glu Ile

980 985 990

Phe Asn Phe Glu Asn Lys Lys Asn Val Lys Tyr Lys Gly Gly Gln Ile

995 1000 1005

Val Glu Lys Tyr Ile Lys Phe Tyr Lys Glu Leu His Gln Asn Asp Glu

1010 1015 1020

Val Lys Ile Asn Lys Tyr Ser Ser Ala Asn Ile Lys Val Leu Lys Gln

1025 1030 1035 1040

Glu Lys Lys Asp Leu Tyr Ile Ala Asn Tyr Ile Ala Ala Phe Asn Tyr

1045 1050 1055

Ile Pro His Ala Glu Ile Ser Leu Leu Glu Val Leu Glu Asn Leu Arg

1060 1065 1070

Lys Leu Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Met Lys Ser

1075 1080 1085

Val Val Asp Ile Leu Lys Glu Tyr Gly Phe Val Ala Thr Phe Lys Ile

1090 1095 1100

Gly Ala Asp Lys Lys Ile Gly Ile Gln Thr Leu Glu Ser Glu Lys Ile

1105 1110 1115 1120

Val His Leu Lys Asn Leu Lys Lys Lys Lys Leu Met Thr Asp Arg Asn

1125 1130 1135

Ser Glu Glu Leu Cys Lys Leu Val Lys Ile Met Phe Glu Tyr Lys Met

1140 1145 1150

Glu Glu Lys Lys Ser Glu Asn

1155

<210> 69

<211> 1232

<212> PRT

<213> 宏基因组(metagenomic)

<400> 69

Met Glu Asn Lys Thr Ser Leu Gly Asn Asn Ile Tyr Tyr Asn Pro Phe

1 5 10 15

Lys Pro Gln Asp Lys Ser Tyr Phe Ala Gly Tyr Phe Asn Ala Ala Met

20 25 30

Glu Asn Thr Asp Ser Val Phe Arg Glu Leu Gly Lys Arg Leu Lys Gly

35 40 45

Lys Glu Tyr Thr Ser Glu Asn Phe Phe Asp Ala Ile Phe Lys Glu Asn

50 55 60

Ile Ser Leu Val Glu Tyr Glu Arg Tyr Val Lys Leu Leu Ser Asp Tyr

65 70 75 80

Phe Pro Met Ala Arg Leu Leu Asp Lys Lys Glu Val Pro Ile Lys Glu

85 90 95

Arg Lys Glu Asn Phe Lys Lys Asn Phe Lys Gly Ile Ile Lys Ala Val

100 105 110

Arg Asp Leu Arg Asn Phe Tyr Thr His Lys Glu His Gly Glu Val Glu

115 120 125

Ile Thr Asp Glu Ile Phe Gly Val Leu Asp Glu Met Leu Lys Ser Thr

130 135 140

Val Leu Thr Val Lys Lys Lys Lys Val Lys Thr Asp Lys Thr Lys Glu

145 150 155 160

Ile Leu Lys Lys Ser Ile Glu Lys Gln Leu Asp Ile Leu Cys Gln Lys

165 170 175

Lys Leu Glu Tyr Leu Arg Asp Thr Ala Arg Lys Ile Glu Glu Lys Arg

180 185 190

Arg Asn Gln Arg Glu Arg Gly Glu Lys Glu Leu Val Ala Pro Phe Lys

195 200 205

Tyr Ser Asp Lys Arg Asp Asp Leu Ile Ala Ala Ile Tyr Asn Asp Ala

210 215 220

Phe Asp Val Tyr Ile Asp Lys Lys Lys Asp Ser Leu Lys Glu Ser Ser

225 230 235 240

Lys Ala Lys Tyr Asn Thr Lys Ser Asp Pro Gln Gln Glu Glu Gly Asp

245 250 255

Leu Lys Ile Pro Ile Ser Lys Asn Gly Val Val Phe Leu Leu Ser Leu

260 265 270

Phe Leu Thr Lys Gln Glu Ile His Ala Phe Lys Ser Lys Ile Ala Gly

275 280 285

Phe Lys Ala Thr Val Ile Asp Glu Ala Thr Val Ser Glu Ala Thr Val

290 295 300

Ser His Gly Lys Asn Ser Ile Cys Phe Met Ala Thr His Glu Ile Phe

305 310 315 320

Ser His Leu Ala Tyr Lys Lys Leu Lys Arg Lys Val Arg Thr Ala Glu

325 330 335

Ile Asn Tyr Gly Glu Ala Glu Asn Ala Glu Gln Leu Ser Val Tyr Ala

340 345 350

Lys Glu Thr Leu Met Met Gln Met Leu Asp Glu Leu Ser Lys Val Pro

355 360 365

Asp Val Val Tyr Gln Asn Leu Ser Glu Asp Val Gln Lys Thr Phe Ile

370 375 380

Glu Asp Trp Asn Glu Tyr Leu Lys Glu Asn Asn Gly Asp Val Gly Thr

385 390 395 400

Met Glu Glu Glu Gln Val Ile His Pro Val Ile Arg Lys Arg Tyr Glu

405 410 415

Asp Lys Phe Asn Tyr Phe Ala Ile Arg Phe Leu Asp Glu Phe Ala Gln

420 425 430

Phe Pro Thr Leu Arg Phe Gln Val His Leu Gly Asn Tyr Leu His Asp

435 440 445

Ser Arg Pro Lys Glu Asn Leu Ile Ser Asp Arg Arg Ile Lys Glu Lys

450 455 460

Ile Thr Val Phe Gly Arg Leu Ser Glu Leu Glu His Lys Lys Ala Leu

465 470 475 480

Phe Ile Lys Asn Thr Glu Thr Asn Glu Asp Arg Glu His Tyr Trp Glu

485 490 495

Ile Phe Pro Asn Pro Asn Tyr Asp Phe Pro Lys Glu Asn Ile Ser Val

500 505 510

Asn Asp Lys Asp Phe Pro Ile Ala Gly Ser Ile Leu Asp Arg Glu Lys

515 520 525

Gln Pro Val Ala Gly Lys Ile Gly Ile Lys Val Lys Leu Leu Asn Gln

530 535 540

Gln Tyr Val Ser Glu Val Asp Lys Ala Val Lys Ala His Gln Leu Lys

545 550 555 560

Gln Arg Lys Ala Ser Lys Pro Ser Ile Gln Asn Ile Ile Glu Glu Ile

565 570 575

Val Pro Ile Asn Glu Ser Asn Pro Lys Glu Ala Ile Val Phe Gly Gly

580 585 590

Gln Pro Thr Ala Tyr Leu Ser Met Asn Asp Ile His Ser Ile Leu Tyr

595 600 605

Glu Phe Phe Asp Lys Trp Glu Lys Lys Lys Glu Lys Leu Glu Lys Lys

610 615 620

Gly Glu Lys Glu Leu Arg Lys Glu Ile Gly Lys Glu Leu Glu Lys Lys

625 630 635 640

Ile Val Gly Lys Ile Gln Ala Gln Ile Gln Gln Ile Ile Asp Lys Asp

645 650 655

Thr Asn Ala Lys Ile Leu Lys Pro Tyr Gln Asp Gly Asn Ser Thr Ala

660 665 670

Ile Asp Lys Glu Lys Leu Ile Lys Asp Leu Lys Gln Glu Gln Asn Ile

675 680 685

Leu Gln Lys Leu Lys Asp Glu Gln Thr Val Arg Glu Lys Glu Tyr Asn

690 695 700

Asp Phe Ile Ala Tyr Gln Asp Lys Asn Arg Glu Ile Asn Lys Val Arg

705 710 715 720

Asp Arg Asn His Lys Gln Tyr Leu Lys Asp Asn Leu Lys Arg Lys Tyr

725 730 735

Pro Glu Ala Pro Ala Arg Lys Glu Val Leu Tyr Tyr Arg Glu Lys Gly

740 745 750

Lys Val Ala Val Trp Leu Ala Asn Asp Ile Lys Arg Phe Met Pro Thr

755 760 765

Asp Phe Lys Asn Glu Trp Lys Gly Glu Gln His Ser Leu Leu Gln Lys

770 775 780

Ser Leu Ala Tyr Tyr Glu Gln Cys Lys Glu Glu Leu Lys Asn Leu Leu

785 790 795 800

Pro Glu Lys Val Phe Gln His Leu Pro Phe Lys Leu Gly Gly Tyr Phe

805 810 815

Gln Gln Lys Tyr Leu Tyr Gln Phe Tyr Thr Cys Tyr Leu Asp Lys Arg

820 825 830

Leu Glu Tyr Ile Ser Gly Leu Val Gln Gln Ala Glu Asn Phe Lys Ser

835 840 845

Glu Asn Lys Val Phe Lys Lys Val Glu Asn Glu Cys Phe Lys Phe Leu

850 855 860

Lys Lys Gln Asn Tyr Thr His Lys Glu Leu Asp Ala Arg Val Gln Ser

865 870 875 880

Ile Leu Gly Tyr Pro Ile Phe Leu Glu Arg Gly Phe Met Asp Glu Lys

885 890 895

Pro Thr Ile Ile Lys Gly Lys Thr Phe Lys Gly Asn Glu Ala Leu Phe

900 905 910

Ala Asp Trp Phe Arg Tyr Tyr Lys Glu Tyr Gln Asn Phe Gln Thr Phe

915 920 925

Tyr Asp Thr Glu Asn Tyr Pro Leu Val Glu Leu Glu Lys Lys Gln Ala

930 935 940

Asp Arg Lys Arg Lys Thr Lys Ile Tyr Gln Gln Lys Lys Asn Asp Val

945 950 955 960

Phe Thr Leu Leu Met Ala Lys His Ile Phe Lys Ser Val Phe Lys Gln

965 970 975

Asp Ser Ile Asp Gln Phe Ser Leu Glu Asp Leu Tyr Gln Ser Arg Glu

980 985 990

Glu Arg Leu Gly Asn Gln Glu Arg Ala Arg Gln Thr Gly Glu Arg Asn

995 1000 1005

Thr Asn Tyr Ile Trp Asn Lys Thr Val Asp Leu Lys Leu Cys Asp Gly

1010 1015 1020

Lys Ile Thr Val Glu Asn Val Lys Leu Lys Asn Val Gly Asp Phe Ile

1025 1030 1035 1040

Lys Tyr Glu Tyr Asp Gln Arg Val Gln Ala Phe Leu Lys Tyr Glu Glu

1045 1050 1055

Asn Ile Glu Trp Gln Ala Phe Leu Ile Lys Glu Ser Lys Glu Glu Glu

1060 1065 1070

Asn Tyr Pro Tyr Val Val Glu Arg Glu Ile Glu Gln Tyr Glu Lys Val

1075 1080 1085

Arg Arg Glu Glu Leu Leu Lys Glu Val His Leu Ile Glu Glu Tyr Ile

1090 1095 1100

Leu Glu Lys Val Lys Asp Lys Glu Ile Leu Lys Lys Gly Asp Asn Gln

1105 1110 1115 1120

Asn Phe Lys Tyr Tyr Ile Leu Asn Gly Leu Leu Lys Gln Leu Lys Asn

1125 1130 1135

Glu Asp Val Glu Ser Tyr Lys Val Phe Asn Leu Asn Thr Glu Pro Glu

1140 1145 1150

Asp Val Asn Ile Asn Gln Leu Lys Gln Glu Ala Thr Asp Leu Glu Gln

1155 1160 1165

Lys Ala Phe Val Leu Thr Tyr Ile Ala Asn Lys Phe Ala His Asn Gln

1170 1175 1180

Leu Pro Lys Lys Glu Phe Trp Asp Tyr Cys Gln Glu Lys Tyr Gly Lys

1185 1190 1195 1200

Ile Glu Lys Glu Lys Thr Tyr Ala Glu Tyr Phe Ala Glu Val Phe Lys

1205 1210 1215

Lys Glu Lys Glu Ala Leu Ile Lys Leu Glu His His His His His His

1220 1225 1230

<210> 70

<211> 954

<212> PRT

<213> 宏基因组(metagenomic)

<400> 70

Met Gly Lys Lys Ile His Ala Arg Asp Leu Arg Glu Gln Arg Lys Thr

1 5 10 15

Asp Arg Thr Glu Lys Phe Ala Asp Gln Asn Lys Lys Arg Glu Ala Glu

20 25 30

Arg Ala Val Pro Lys Lys Asp Ala Ala Val Ser Val Lys Ser Val Ser

35 40 45

Ser Val Ser Ser Lys Lys Asp Asn Val Thr Lys Ser Met Ala Lys Ala

50 55 60

Ala Gly Val Lys Ser Val Phe Ala Val Gly Asn Thr Val Tyr Met Thr

65 70 75 80

Ser Phe Gly Arg Gly Asn Asp Ala Val Leu Glu Gln Lys Ile Val Asp

85 90 95

Thr Ser His Glu Pro Leu Asn Ile Asp Asp Pro Ala Tyr Gln Leu Asn

100 105 110

Val Val Thr Met Asn Gly Tyr Ser Val Thr Gly His Arg Gly Glu Thr

115 120 125

Val Ser Ala Val Thr Asp Asn Pro Leu Arg Arg Phe Asn Gly Arg Lys

130 135 140

Lys Asp Glu Pro Glu Gln Ser Val Pro Thr Asp Met Leu Cys Leu Lys

145 150 155 160

Pro Thr Leu Glu Lys Lys Phe Phe Gly Lys Glu Phe Asp Asp Asn Ile

165 170 175

His Ile Gln Leu Ile Tyr Asn Ile Leu Asp Ile Glu Lys Ile Leu Ala

180 185 190

Val Tyr Ser Thr Asn Ala Ile Tyr Ala Leu Asn Asn Met Ser Ala Asp

195 200 205

Glu Asn Ile Glu Asn Ser Asp Phe Phe Met Lys Arg Thr Thr Asp Glu

210 215 220

Thr Phe Asp Asp Phe Glu Lys Lys Lys Glu Ser Thr Asn Ser Arg Glu

225 230 235 240

Lys Ala Asp Phe Asp Ala Phe Glu Lys Phe Ile Gly Asn Tyr Arg Leu

245 250 255

Ala Tyr Phe Ala Asp Ala Phe Tyr Val Asn Lys Lys Asn Pro Lys Gly

260 265 270

Lys Ala Lys Asn Val Leu Arg Glu Asp Lys Glu Leu Tyr Ser Val Leu

275 280 285

Thr Leu Ile Gly Lys Leu Arg His Trp Cys Val His Ser Glu Glu Gly

290 295 300

Arg Ala Glu Phe Trp Leu Tyr Lys Leu Asp Glu Leu Lys Asp Asp Phe

305 310 315 320

Lys Asn Val Leu Asp Val Val Tyr Asn Arg Pro Val Glu Glu Ile Asn

325 330 335

Asn Arg Phe Ile Glu Asn Asn Lys Val Asn Ile Gln Ile Leu Gly Ser

340 345 350

Val Tyr Lys Asn Thr Asp Ile Ala Glu Leu Val Arg Ser Tyr Tyr Glu

355 360 365

Phe Leu Ile Thr Lys Lys Tyr Lys Asn Met Gly Phe Ser Ile Lys Lys

370 375 380

Leu Arg Glu Ser Met Leu Glu Gly Lys Gly Tyr Ala Asp Lys Glu Tyr

385 390 395 400

Asp Ser Val Arg Asn Lys Leu Tyr Gln Met Thr Asp Phe Ile Leu Tyr

405 410 415

Thr Gly Tyr Ile Asn Glu Asp Ser Asp Arg Ala Asp Asp Leu Val Asn

420 425 430

Thr Leu Arg Ser Ser Leu Lys Glu Asp Asp Lys Thr Thr Val Tyr Cys

435 440 445

Lys Glu Ala Asp Tyr Leu Trp Lys Lys Tyr Arg Glu Ser Ile Arg Glu

450 455 460

Val Ala Asp Ala Leu Asp Gly Asp Asn Ile Lys Lys Leu Ser Lys Ser

465 470 475 480

Asn Ile Glu Ile Gln Glu Asp Lys Leu Arg Lys Cys Phe Ile Ser Tyr

485 490 495

Ala Asp Ser Val Ser Glu Phe Thr Lys Leu Ile Tyr Leu Leu Thr Arg

500 505 510

Phe Leu Ser Gly Lys Glu Ile Asn Asp Leu Val Thr Thr Leu Ile Asn

515 520 525

Lys Phe Asp Asn Ile Arg Ser Phe Leu Glu Ile Met Asp Glu Leu Gly

530 535 540

Leu Asp Arg Thr Phe Thr Ala Glu Tyr Ser Phe Phe Glu Gly Ser Thr

545 550 555 560

Lys Tyr Leu Ala Glu Leu Val Glu Leu Asn Ser Phe Val Lys Ser Cys

565 570 575

Ser Phe Asp Ile Asn Ala Lys Arg Thr Met Tyr Arg Asp Ala Leu Asp

580 585 590

Ile Leu Gly Ile Glu Ser Asp Lys Thr Glu Glu Asp Ile Glu Lys Met

595 600 605

Ile Asp Asn Ile Leu Gln Ile Asp Ala Asn Gly Asp Lys Lys Leu Lys

610 615 620

Lys Asn Asn Gly Leu Arg Asn Phe Ile Ala Ser Asn Val Ile Asp Ser

625 630 635 640

Asn Arg Phe Lys Tyr Leu Val Arg Tyr Gly Asn Pro Lys Lys Ile Arg

645 650 655

Glu Thr Ala Lys Cys Lys Pro Ala Val Arg Phe Val Leu Asn Glu Ile

660 665 670

Pro Asp Ala Gln Ile Glu Arg Tyr Tyr Glu Ala Cys Cys Pro Lys Asn

675 680 685

Thr Ala Leu Cys Ser Ala Asn Lys Arg Arg Glu Lys Leu Ala Asp Met

690 695 700

Ile Ala Glu Ile Lys Phe Glu Asn Phe Ser Asp Ala Gly Asn Tyr Gln

705 710 715 720

Lys Ala Asn Val Thr Ser Arg Thr Ser Glu Ala Glu Ile Lys Arg Lys

725 730 735

Asn Gln Ala Ile Ile Arg Leu Tyr Leu Thr Val Met Tyr Ile Met Leu

740 745 750

Lys Asn Leu Val Asn Val Asn Ala Arg Tyr Val Ile Ala Phe His Cys

755 760 765

Val Glu Arg Asp Thr Lys Leu Tyr Ala Glu Ser Gly Leu Glu Val Gly

770 775 780

Asn Ile Glu Lys Asn Lys Thr Asn Leu Thr Met Ala Val Met Gly Val

785 790 795 800

Lys Leu Glu Asn Gly Ile Ile Lys Thr Glu Phe Asp Lys Ser Phe Ala

805 810 815

Glu Asn Ala Ala Asn Arg Tyr Leu Arg Asn Ala Arg Trp Tyr Lys Leu

820 825 830

Ile Leu Asp Asn Leu Lys Lys Ser Glu Arg Ala Val Val Asn Glu Phe

835 840 845

Arg Asn Thr Val Cys His Leu Asn Ala Ile Arg Asn Ile Asn Ile Asn

850 855 860

Ile Lys Glu Ile Lys Glu Val Glu Asn Tyr Phe Ala Leu Tyr His Tyr

865 870 875 880

Leu Ile Gln Lys His Leu Glu Asn Arg Phe Ala Asp Lys Lys Val Glu

885 890 895

Arg Asp Thr Gly Asp Phe Ile Ser Lys Leu Glu Glu His Lys Thr Tyr

900 905 910

Cys Lys Asp Phe Val Lys Ala Tyr Cys Thr Pro Phe Gly Tyr Asn Leu

915 920 925

Val Arg Tyr Lys Asn Leu Thr Ile Asp Gly Leu Phe Asp Lys Asn Tyr

930 935 940

Pro Gly Lys Asp Asp Ser Asp Glu Gln Lys

945 950

<210> 71

<211> 930

<212> PRT

<213> 宏基因组(metagenomic)

<400> 71

Met Ala Lys Lys Asn Lys Met Lys Pro Arg Glu Leu Arg Glu Ala Gln

1 5 10 15

Lys Lys Ala Arg Gln Leu Lys Ala Ala Glu Ile Asn Asn Asn Ala Ala

20 25 30

Pro Ala Ile Ala Ala Met Pro Ala Ala Glu Val Ile Ala Pro Val Ala

35 40 45

Glu Lys Lys Lys Ser Ser Val Lys Ala Ala Gly Met Lys Ser Ile Leu

50 55 60

Val Ser Glu Asn Lys Met Tyr Ile Thr Ser Phe Gly Lys Gly Asn Ser

65 70 75 80

Ala Val Leu Glu Tyr Glu Val Asp Asn Asn Asp Tyr Asn Lys Thr Gln

85 90 95

Leu Ser Ser Lys Asp Asn Ser Asn Ile Glu Leu Gly Asp Val Asn Glu

100 105 110

Val Asn Ile Thr Phe Ser Ser Lys His Gly Phe Gly Ser Gly Val Glu

115 120 125

Ile Asn Thr Ser Asn Pro Thr His Arg Ser Gly Glu Ser Ser Pro Val

130 135 140

Arg Gly Asp Met Leu Gly Leu Lys Ser Glu Leu Glu Lys Arg Phe Phe

145 150 155 160

Gly Lys Thr Phe Asp Asp Asn Ile His Ile Gln Leu Ile Tyr Asn Ile

165 170 175

Leu Asp Ile Glu Lys Ile Leu Ala Val Tyr Val Thr Asn Ile Val Tyr

180 185 190

Ala Leu Asn Asn Met Leu Gly Ile Lys Asp Ser Glu Ser Tyr Asp Asp

195 200 205

Phe Met Gly Tyr Leu Ser Ala Arg Asn Thr Tyr Glu Val Phe Thr His

210 215 220

Pro Asp Lys Ser Asn Leu Ser Asp Lys Val Lys Gly Asn Ile Lys Lys

225 230 235 240

Ser Leu Ser Lys Phe Asn Asp Leu Leu Lys Thr Lys Arg Leu Gly Tyr

245 250 255

Phe Gly Leu Glu Glu Pro Lys Thr Lys Asp Thr Arg Ala Ser Glu Ala

260 265 270

Tyr Lys Lys Arg Val Tyr His Met Leu Ala Ile Val Gly Gln Ile Ala

275 280 285

Gln Cys Val Phe His Asp Lys Ser Gly Ala Lys Arg Phe Asp Leu Tyr

290 295 300

Ser Phe Ile Asn Asn Ile Asp Pro Glu Tyr Arg Asp Thr Leu Asp Tyr

305 310 315 320

Leu Val Glu Glu Arg Leu Lys Ser Ile Asn Lys Asp Phe Ile Glu Gly

325 330 335

Asn Lys Val Asn Ile Ser Leu Leu Ile Asp Met Met Lys Gly Tyr Glu

340 345 350

Ala Asp Asp Ile Ile Arg Leu Tyr Tyr Asp Phe Ile Val Leu Lys Ser

355 360 365

Gln Lys Asn Leu Gly Phe Ser Ile Lys Lys Leu Arg Glu Lys Met Leu

370 375 380

Glu Glu Tyr Gly Phe Arg Phe Lys Asp Lys Gln Tyr Asp Ser Val Arg

385 390 395 400

Ser Lys Met Tyr Lys Leu Met Asp Phe Leu Leu Phe Cys Asn Tyr Tyr

405 410 415

Arg Asn Asp Val Ala Ala Gly Glu Ala Leu Val Arg Lys Leu Arg Phe

420 425 430

Ser Met Thr Asp Asp Glu Lys Glu Gly Ile Tyr Ala Asp Glu Ala Ala

435 440 445

Lys Leu Trp Gly Lys Phe Arg Asn Asp Phe Glu Asn Ile Ala Asp His

450 455 460

Met Asn Gly Asp Val Ile Lys Glu Leu Gly Lys Ala Asp Met Asp Phe

465 470 475 480

Asp Glu Lys Ile Leu Asp Ser Glu Lys Lys Asn Ala Ser Asp Leu Leu

485 490 495

Tyr Phe Ser Lys Met Ile Tyr Met Leu Thr Tyr Phe Leu Asp Gly Lys

500 505 510

Glu Ile Asn Asp Leu Leu Thr Thr Leu Ile Ser Lys Phe Asp Asn Ile

515 520 525

Lys Glu Phe Leu Lys Ile Met Lys Ser Ser Ala Val Asp Val Glu Cys

530 535 540

Glu Leu Thr Ala Gly Tyr Lys Leu Phe Asn Asp Ser Gln Arg Ile Thr

545 550 555 560

Asn Glu Leu Phe Ile Val Lys Asn Ile Ala Ser Met Arg Lys Pro Ala

565 570 575

Ala Ser Ala Lys Leu Thr Met Phe Arg Asp Ala Leu Thr Ile Leu Gly

580 585 590

Ile Asp Asp Asn Ile Thr Asp Asp Arg Ile Ser Glu Ile Leu Lys Leu

595 600 605

Lys Glu Lys Gly Lys Gly Ile His Gly Leu Arg Asn Phe Ile Thr Asn

610 615 620

Asn Val Ile Glu Ser Ser Arg Phe Val Tyr Leu Ile Lys Tyr Ala Asn

625 630 635 640

Ala Gln Lys Ile Arg Glu Val Ala Lys Asn Glu Lys Val Val Met Phe

645 650 655

Val Leu Gly Gly Ile Pro Asp Thr Gln Ile Glu Arg Tyr Tyr Lys Ser

660 665 670

Cys Val Glu Phe Pro Asp Met Asn Ser Ser Leu Glu Ala Lys Arg Ser

675 680 685

Glu Leu Ala Arg Met Ile Lys Asn Ile Ser Phe Asp Asp Phe Lys Asn

690 695 700

Val Lys Gln Gln Ala Lys Gly Arg Glu Asn Val Ala Lys Glu Arg Ala

705 710 715 720

Lys Ala Val Ile Gly Leu Tyr Leu Thr Val Met Tyr Leu Leu Val Lys

725 730 735

Asn Leu Val Asn Val Asn Ala Arg Tyr Val Ile Ala Ile His Cys Leu

740 745 750

Glu Arg Asp Phe Gly Leu Tyr Lys Glu Ile Ile Pro Glu Leu Ala Ser

755 760 765

Lys Asn Leu Lys Asn Asp Tyr Arg Ile Leu Ser Gln Thr Leu Cys Glu

770 775 780

Leu Cys Asp Asp Arg Asn Glu Ser Ser Asn Leu Phe Leu Lys Lys Asn

785 790 795 800

Lys Arg Leu Arg Lys Cys Val Glu Val Asp Ile Asn Asn Ala Asp Ser

805 810 815

Ser Met Thr Arg Lys Tyr Ala Asn Cys Ile Ala His Leu Thr Val Val

820 825 830

Arg Glu Leu Lys Glu Tyr Ile Gly Asp Ile Arg Thr Val Asp Ser Tyr

835 840 845

Phe Ser Ile Tyr His Tyr Val Met Gln Arg Cys Ile Thr Lys Arg Gly

850 855 860

Asp Asp Thr Lys Gln Glu Glu Lys Ile Lys Tyr Glu Asp Asp Leu Leu

865 870 875 880

Lys Asn His Gly Tyr Thr Lys Asp Phe Val Lys Ala Leu Asn Ser Pro

885 890 895

Phe Gly Tyr Asn Ile Pro Arg Phe Lys Asn Leu Ser Ile Glu Gln Leu

900 905 910

Phe Asp Arg Asn Glu Tyr Leu Thr Glu Lys Leu Glu His His His His

915 920 925

His His

930

<210> 72

<211> 1087

<212> PRT

<213> 宏基因组(metagenomic)

<400> 72

Thr Met Arg Ile Thr Lys Val Glu Val Asp Arg Lys Lys Val Leu Ile

1 5 10 15

Ser Arg Asp Lys Asn Gly Gly Lys Leu Val Tyr Glu Asn Glu Met Gln

20 25 30

Asp Asn Thr Glu Gln Ile Met His His Lys Lys Ser Ser Phe Tyr Lys

35 40 45

Ser Val Val Asn Lys Thr Ile Cys Arg Pro Glu Gln Lys Gln Met Lys

50 55 60

Lys Leu Val His Gly Leu Leu Gln Glu Asn Ser Gln Glu Lys Ile Lys

65 70 75 80

Val Ser Asp Val Thr Lys Leu Asn Ile Ser Asn Phe Leu Asn His Arg

85 90 95

Phe Lys Lys Ser Leu Tyr Tyr Phe Pro Glu Asn Ser Pro Asp Lys Ser

100 105 110

Glu Glu Tyr Arg Ile Glu Ile Asn Leu Ser Gln Leu Leu Glu Asp Ser

115 120 125

Leu Lys Lys Gln Gln Gly Thr Phe Ile Cys Trp Glu Ser Phe Ser Lys

130 135 140

Asp Met Glu Leu Tyr Ile Asn Trp Ala Glu Asn Tyr Ile Ser Ser Lys

145 150 155 160

Thr Lys Leu Ile Lys Lys Ser Ile Arg Asn Asn Arg Ile Gln Ser Thr

165 170 175

Glu Ser Arg Ser Gly Gln Leu Met Asp Arg Tyr Met Lys Asp Ile Leu

180 185 190

Asn Lys Asn Lys Pro Phe Asp Ile Gln Ser Val Ser Glu Lys Tyr Gln

195 200 205

Leu Glu Lys Leu Thr Ser Ala Leu Lys Ala Thr Phe Lys Glu Ala Lys

210 215 220

Lys Asn Asp Lys Glu Ile Asn Tyr Lys Leu Lys Ser Thr Leu Gln Asn

225 230 235 240

His Glu Arg Gln Ile Ile Glu Glu Leu Lys Glu Asn Ser Glu Leu Asn

245 250 255

Gln Phe Asn Ile Glu Ile Arg Lys His Leu Glu Thr Tyr Phe Pro Ile

260 265 270

Lys Lys Thr Asn Arg Lys Val Gly Asp Ile Arg Asn Leu Glu Ile Gly

275 280 285

Glu Ile Gln Lys Ile Val Asn His Arg Leu Lys Asn Lys Ile Val Gln

290 295 300

Arg Ile Leu Gln Glu Gly Lys Leu Ala Ser Tyr Glu Ile Glu Ser Thr

305 310 315 320

Val Asn Ser Asn Ser Leu Gln Lys Ile Lys Ile Glu Glu Ala Phe Ala

325 330 335

Leu Lys Phe Ile Asn Ala Cys Leu Phe Ala Ser Asn Asn Leu Arg Asn

340 345 350

Met Val Tyr Pro Val Cys Lys Lys Asp Ile Leu Met Ile Gly Glu Phe

355 360 365

Lys Asn Ser Phe Lys Glu Ile Lys His Lys Lys Phe Ile Arg Gln Trp

370 375 380

Ser Gln Phe Phe Ser Gln Glu Ile Thr Val Asp Asp Ile Glu Leu Ala

385 390 395 400

Ser Trp Gly Leu Arg Gly Ala Ile Ala Pro Ile Arg Asn Glu Ile Ile

405 410 415

His Leu Lys Lys His Ser Trp Lys Lys Phe Phe Asn Asn Pro Thr Phe

420 425 430

Lys Val Lys Lys Ser Lys Ile Ile Asn Gly Lys Thr Lys Asp Val Thr

435 440 445

Ser Glu Phe Leu Tyr Lys Glu Thr Leu Phe Lys Asp Tyr Phe Tyr Ser

450 455 460

Glu Leu Asp Ser Val Pro Glu Leu Ile Ile Asn Lys Met Glu Ser Ser

465 470 475 480

Lys Ile Leu Asp Tyr Tyr Ser Ser Asp Gln Leu Asn Gln Val Phe Thr

485 490 495

Ile Pro Asn Phe Glu Leu Ser Leu Leu Thr Ser Ala Val Pro Phe Ala

500 505 510

Pro Ser Phe Lys Arg Val Tyr Leu Lys Gly Phe Asp Tyr Gln Asn Gln

515 520 525

Asp Glu Ala Gln Pro Asp Tyr Asn Leu Lys Leu Asn Ile Tyr Asn Glu

530 535 540

Lys Ala Phe Asn Ser Glu Ala Phe Gln Ala Gln Tyr Ser Leu Phe Lys

545 550 555 560

Met Val Tyr Tyr Gln Val Phe Leu Pro Gln Phe Thr Thr Asn Asn Asp

565 570 575

Leu Phe Lys Ser Ser Val Asp Phe Ile Leu Thr Leu Asn Lys Glu Arg

580 585 590

Lys Gly Tyr Ala Lys Ala Phe Gln Asp Ile Arg Lys Met Asn Lys Asp

595 600 605

Glu Lys Pro Ser Glu Tyr Met Ser Tyr Ile Gln Ser Gln Leu Met Leu

610 615 620

Tyr Gln Lys Lys Gln Glu Glu Lys Glu Lys Ile Asn His Phe Glu Lys

625 630 635 640

Phe Ile Asn Gln Val Phe Ile Lys Gly Phe Asn Ser Phe Ile Glu Lys

645 650 655

Asn Arg Leu Thr Tyr Ile Cys His Pro Thr Lys Asn Thr Val Pro Glu

660 665 670

Asn Asp Asn Ile Glu Ile Pro Phe His Thr Asp Met Asp Asp Ser Asn

675 680 685

Ile Ala Phe Trp Leu Met Cys Lys Leu Leu Asp Ala Lys Gln Leu Ser

690 695 700

Glu Leu Arg Asn Glu Met Ile Lys Phe Ser Cys Ser Leu Gln Ser Thr

705 710 715 720

Glu Glu Ile Ser Thr Phe Thr Lys Ala Arg Glu Val Ile Gly Leu Ala

725 730 735

Leu Leu Asn Gly Glu Lys Gly Cys Asn Asp Trp Lys Glu Leu Phe Asp

740 745 750

Asp Lys Glu Ala Trp Lys Lys Asn Met Ser Leu Tyr Val Ser Glu Glu

755 760 765

Leu Leu Gln Ser Leu Pro Tyr Thr Gln Glu Asp Gly Gln Thr Pro Val

770 775 780

Ile Asn Arg Ser Ile Asp Leu Val Lys Lys Tyr Gly Thr Glu Thr Ile

785 790 795 800

Leu Glu Lys Leu Phe Ser Ser Ser Asp Asp Tyr Lys Val Ser Ala Lys

805 810 815

Asp Ile Ala Lys Leu His Glu Tyr Asp Val Thr Glu Lys Ile Ala Gln

820 825 830

Gln Glu Ser Leu His Lys Gln Trp Ile Glu Lys Pro Gly Leu Ala Arg

835 840 845

Asp Ser Ala Trp Thr Lys Lys Tyr Gln Asn Val Ile Asn Asp Ile Ser

850 855 860

Asn Tyr Gln Trp Ala Lys Thr Lys Val Glu Leu Thr Gln Val Arg His

865 870 875 880

Leu His Gln Leu Thr Ile Asp Leu Leu Ser Arg Leu Ala Gly Tyr Met

885 890 895

Ser Ile Ala Asp Arg Asp Phe Gln Phe Ser Ser Asn Tyr Ile Leu Glu

900 905 910

Arg Glu Asn Ser Glu Tyr Arg Val Thr Ser Trp Ile Leu Leu Ser Glu

915 920 925

Asn Lys Asn Lys Asn Lys Tyr Asn Asp Tyr Glu Leu Tyr Asn Leu Lys

930 935 940

Asn Ala Ser Ile Lys Val Ser Ser Lys Asn Asp Pro Gln Leu Lys Val

945 950 955 960

Asp Leu Lys Gln Leu Arg Leu Thr Leu Glu Tyr Leu Glu Leu Phe Asp

965 970 975

Asn Arg Leu Lys Glu Lys Arg Asn Asn Ile Ser His Phe Asn Tyr Leu

980 985 990

Asn Gly Gln Leu Gly Asn Ser Ile Leu Glu Leu Phe Asp Asp Ala Arg

995 1000 1005

Asp Val Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys Ser

1010 1015 1020

Leu Lys Glu Ile Leu Ser Ser His Gly Met Glu Val Thr Phe Lys Pro

1025 1030 1035 1040

Leu Tyr Gln Thr Asn His His Leu Lys Ile Asp Lys Leu Gln Pro Lys

1045 1050 1055

Lys Ile His His Leu Gly Glu Lys Ser Thr Val Ser Ser Asn Gln Val

1060 1065 1070

Ser Asn Glu Tyr Cys Gln Leu Val Arg Thr Leu Leu Thr Met Lys

1075 1080 1085

<210> 73

<211> 1126

<212> PRT

<213> 宏基因组(metagenomic)

<400> 73

Met Trp Ile Ser Ile Lys Thr Leu Ile His His Leu Gly Val Leu Phe

1 5 10 15

Phe Cys Asp Tyr Met Tyr Asn Arg Arg Glu Lys Lys Ile Ile Glu Val

20 25 30

Lys Thr Met Arg Ile Thr Lys Val Glu Val Asp Arg Lys Lys Val Leu

35 40 45

Ile Ser Arg Asp Lys Asn Gly Gly Lys Leu Val Tyr Glu Asn Glu Met

50 55 60

Gln Asp Asn Thr Glu Gln Ile Met His His Lys Lys Ser Ser Phe Tyr

65 70 75 80

Lys Ser Val Val Asn Lys Thr Ile Cys Arg Pro Glu Gln Lys Gln Met

85 90 95

Lys Lys Leu Val His Gly Leu Leu Gln Glu Asn Ser Gln Glu Lys Ile

100 105 110

Lys Val Ser Asp Val Thr Lys Leu Asn Ile Ser Asn Phe Leu Asn His

115 120 125

Arg Phe Lys Lys Ser Leu Tyr Tyr Phe Pro Glu Asn Ser Pro Asp Lys

130 135 140

Ser Glu Glu Tyr Arg Ile Glu Ile Asn Leu Ser Gln Leu Leu Glu Asp

145 150 155 160

Ser Leu Lys Lys Gln Gln Gly Thr Phe Ile Cys Trp Glu Ser Phe Ser

165 170 175

Lys Asp Met Glu Leu Tyr Ile Asn Trp Ala Glu Asn Tyr Ile Ser Ser

180 185 190

Lys Thr Lys Leu Ile Lys Lys Ser Ile Arg Asn Asn Arg Ile Gln Ser

195 200 205

Thr Glu Ser Arg Ser Gly Gln Leu Met Asp Arg Tyr Met Lys Asp Ile

210 215 220

Leu Asn Lys Asn Lys Pro Phe Asp Ile Gln Ser Val Ser Glu Lys Tyr

225 230 235 240

Gln Leu Glu Lys Leu Thr Ser Ala Leu Lys Ala Thr Phe Lys Glu Ala

245 250 255

Lys Lys Asn Asp Lys Glu Ile Asn Tyr Lys Leu Lys Ser Thr Leu Gln

260 265 270

Asn His Glu Arg Gln Ile Ile Glu Glu Leu Lys Glu Asn Ser Glu Leu

275 280 285

Asn Gln Phe Asn Ile Glu Ile Arg Lys His Leu Glu Thr Tyr Phe Pro

290 295 300

Ile Lys Lys Thr Asn Arg Lys Val Gly Asp Ile Arg Asn Leu Glu Ile

305 310 315 320

Gly Glu Ile Gln Lys Ile Val Asn His Arg Leu Lys Asn Lys Ile Val

325 330 335

Gln Arg Ile Leu Gln Glu Gly Lys Leu Ala Ser Tyr Glu Ile Glu Ser

340 345 350

Thr Val Asn Ser Asn Ser Leu Gln Lys Ile Lys Ile Glu Glu Ala Phe

355 360 365

Ala Leu Lys Phe Ile Asn Ala Cys Leu Phe Ala Ser Asn Asn Leu Arg

370 375 380

Asn Met Val Tyr Pro Val Cys Lys Lys Asp Ile Leu Met Ile Gly Glu

385 390 395 400

Phe Lys Asn Ser Phe Lys Glu Ile Lys His Lys Lys Phe Ile Arg Gln

405 410 415

Trp Ser Gln Phe Phe Ser Gln Glu Ile Thr Val Asp Asp Ile Glu Leu

420 425 430

Ala Ser Trp Gly Leu Arg Gly Ala Ile Ala Pro Ile Arg Asn Glu Ile

435 440 445

Ile His Leu Lys Lys His Ser Trp Lys Lys Phe Phe Asn Asn Pro Thr

450 455 460

Phe Lys Val Lys Lys Ser Lys Ile Ile Asn Gly Lys Thr Lys Asp Val

465 470 475 480

Thr Ser Glu Phe Leu Tyr Lys Glu Thr Leu Phe Lys Asp Tyr Phe Tyr

485 490 495

Ser Glu Leu Asp Ser Val Pro Glu Leu Ile Ile Asn Lys Met Glu Ser

500 505 510

Ser Lys Ile Leu Asp Tyr Tyr Ser Ser Asp Gln Leu Asn Gln Val Phe

515 520 525

Thr Ile Pro Asn Phe Glu Leu Ser Leu Leu Thr Ser Ala Val Pro Phe

530 535 540

Ala Pro Ser Phe Lys Arg Val Tyr Leu Lys Gly Phe Asp Tyr Gln Asn

545 550 555 560

Gln Asp Glu Ala Gln Pro Asp Tyr Asn Leu Lys Leu Asn Ile Tyr Asn

565 570 575

Glu Lys Ala Phe Asn Ser Glu Ala Phe Gln Ala Gln Tyr Ser Leu Phe

580 585 590

Lys Met Val Tyr Tyr Gln Val Phe Leu Pro Gln Phe Thr Thr Asn Asn

595 600 605

Asp Leu Phe Lys Ser Ser Val Asp Phe Ile Leu Thr Leu Asn Lys Glu

610 615 620

Arg Lys Gly Tyr Ala Lys Ala Phe Gln Asp Ile Arg Lys Met Asn Lys

625 630 635 640

Asp Glu Lys Pro Ser Glu Tyr Met Ser Tyr Ile Gln Ser Gln Leu Met

645 650 655

Leu Tyr Gln Lys Lys Gln Glu Glu Lys Glu Lys Ile Asn His Phe Glu

660 665 670

Lys Phe Ile Asn Gln Val Phe Ile Lys Gly Phe Asn Ser Phe Ile Glu

675 680 685

Lys Asn Arg Leu Thr Tyr Ile Cys His Pro Thr Lys Asn Thr Val Pro

690 695 700

Glu Asn Asp Asn Ile Glu Ile Pro Phe His Thr Asp Met Asp Asp Ser

705 710 715 720

Asn Ile Ala Phe Trp Leu Met Cys Lys Leu Leu Asp Ala Lys Gln Leu

725 730 735

Ser Glu Leu Arg Asn Glu Met Ile Lys Phe Ser Cys Ser Leu Gln Ser

740 745 750

Thr Glu Glu Ile Ser Thr Phe Thr Lys Ala Arg Glu Val Ile Gly Leu

755 760 765

Ala Leu Leu Asn Gly Glu Lys Gly Cys Asn Asp Trp Lys Glu Leu Phe

770 775 780

Asp Asp Lys Glu Ala Trp Lys Lys Asn Met Ser Leu Tyr Val Ser Glu

785 790 795 800

Glu Leu Leu Gln Ser Leu Pro Tyr Thr Gln Glu Asp Gly Gln Thr Pro

805 810 815

Val Ile Asn Arg Ser Ile Asp Leu Val Lys Lys Tyr Gly Thr Glu Thr

820 825 830

Ile Leu Glu Lys Leu Phe Ser Ser Ser Asp Asp Tyr Lys Val Ser Ala

835 840 845

Lys Asp Ile Ala Lys Leu His Glu Tyr Asp Val Thr Glu Lys Ile Ala

850 855 860

Gln Gln Glu Ser Leu His Lys Gln Trp Ile Glu Lys Pro Gly Leu Ala

865 870 875 880

Arg Asp Ser Ala Trp Thr Lys Lys Tyr Gln Asn Val Ile Asn Asp Ile

885 890 895

Ser Asn Tyr Gln Trp Ala Lys Thr Lys Val Glu Leu Thr Gln Val Arg

900 905 910

His Leu His Gln Leu Thr Ile Asp Leu Leu Ser Arg Leu Ala Gly Tyr

915 920 925

Met Ser Ile Ala Asp Arg Asp Phe Gln Phe Ser Ser Asn Tyr Ile Leu

930 935 940

Glu Arg Glu Asn Ser Glu Tyr Arg Val Thr Ser Trp Ile Leu Leu Ser

945 950 955 960

Glu Asn Lys Asn Lys Asn Lys Tyr Asn Asp Tyr Glu Leu Tyr Asn Leu

965 970 975

Lys Asn Ala Ser Ile Lys Val Ser Ser Lys Asn Asp Pro Gln Leu Lys

980 985 990

Val Asp Leu Lys Gln Leu Arg Leu Thr Leu Glu Tyr Leu Glu Leu Phe

995 1000 1005

Asp Asn Arg Leu Lys Glu Lys Arg Asn Asn Ile Ser His Phe Asn Tyr

1010 1015 1020

Leu Asn Gly Gln Leu Gly Asn Ser Ile Leu Glu Leu Phe Asp Asp Ala

1025 1030 1035 1040

Arg Asp Val Leu Ser Tyr Asp Arg Lys Leu Lys Asn Ala Val Ser Lys

1045 1050 1055

Ser Leu Lys Glu Ile Leu Ser Ser His Gly Met Glu Val Thr Phe Lys

1060 1065 1070

Pro Leu Tyr Gln Thr Asn His His Leu Lys Ile Asp Lys Leu Gln Pro

1075 1080 1085

Lys Lys Ile His His Leu Gly Glu Lys Ser Thr Val Ser Ser Asn Gln

1090 1095 1100

Val Ser Asn Glu Tyr Cys Gln Leu Val Arg Thr Leu Leu Thr Met Lys

1105 1110 1115 1120

His His His His His His

1125

<210> 74

<211> 1391

<212> PRT

<213> 宏基因组(metagenomic)

<400> 74

Gly Ser Met Gly Asn Leu Phe Gly His Lys Arg Trp Tyr Glu Val Arg

1 5 10 15

Asp Lys Lys Asp Phe Lys Ile Lys Arg Lys Val Lys Val Lys Arg Asn

20 25 30

Tyr Asp Gly Asn Lys Tyr Ile Leu Asn Ile Asn Glu Asn Asn Asn Lys

35 40 45

Glu Lys Ile Asp Asn Asn Lys Phe Ile Arg Lys Tyr Ile Asn Tyr Lys

50 55 60

Lys Asn Asp Asn Ile Leu Lys Glu Phe Thr Arg Lys Phe His Ala Gly

65 70 75 80

Asn Ile Leu Phe Lys Leu Lys Gly Lys Glu Gly Ile Ile Arg Ile Glu

85 90 95

Asn Asn Asp Asp Phe Leu Glu Thr Glu Glu Val Val Leu Tyr Ile Glu

100 105 110

Ala Tyr Gly Lys Ser Glu Lys Leu Lys Ala Leu Gly Ile Thr Lys Lys

115 120 125

Lys Ile Ile Asp Glu Ala Ile Arg Gln Gly Ile Thr Lys Asp Asp Lys

130 135 140

Lys Ile Glu Ile Lys Arg Gln Glu Asn Glu Glu Glu Ile Glu Ile Asp

145 150 155 160

Ile Arg Asp Glu Tyr Thr Asn Lys Thr Leu Asn Asp Cys Ser Ile Ile

165 170 175

Leu Arg Ile Ile Glu Asn Asp Glu Leu Glu Thr Lys Lys Ser Ile Tyr

180 185 190

Glu Ile Phe Lys Asn Ile Asn Met Ser Leu Tyr Lys Ile Ile Glu Lys

195 200 205

Ile Ile Glu Asn Glu Thr Glu Lys Val Phe Glu Asn Arg Tyr Tyr Glu

210 215 220

Glu His Leu Arg Glu Lys Leu Leu Lys Asp Asp Lys Ile Asp Val Ile

225 230 235 240

Leu Thr Asn Phe Met Glu Ile Arg Glu Lys Ile Lys Ser Asn Leu Glu

245 250 255

Ile Leu Gly Phe Val Lys Phe Tyr Leu Asn Val Gly Gly Asp Lys Lys

260 265 270

Lys Ser Lys Asn Lys Lys Met Leu Val Glu Lys Ile Leu Asn Ile Asn

275 280 285

Val Asp Leu Thr Val Glu Asp Ile Ala Asp Phe Val Ile Lys Glu Leu

290 295 300

Glu Phe Trp Asn Ile Thr Lys Arg Ile Glu Lys Val Lys Lys Val Asn

305 310 315 320

Asn Glu Phe Leu Glu Lys Arg Arg Asn Arg Thr Tyr Ile Lys Ser Tyr

325 330 335

Val Leu Leu Asp Lys His Glu Lys Phe Lys Ile Glu Arg Glu Asn Lys

340 345 350

Lys Asp Lys Ile Val Lys Phe Phe Val Glu Asn Ile Lys Asn Asn Ser

355 360 365

Ile Lys Glu Lys Ile Glu Lys Ile Leu Ala Glu Phe Lys Ile Asp Glu

370 375 380

Leu Ile Lys Lys Leu Glu Lys Glu Leu Lys Lys Gly Asn Cys Asp Thr

385 390 395 400

Glu Ile Phe Gly Ile Phe Lys Lys His Tyr Lys Val Asn Phe Asp Ser

405 410 415

Lys Lys Phe Ser Lys Lys Ser Asp Glu Glu Lys Glu Leu Tyr Lys Ile

420 425 430

Ile Tyr Arg Tyr Leu Lys Gly Arg Ile Glu Lys Ile Leu Val Asn Glu

435 440 445

Gln Lys Val Arg Leu Lys Lys Met Glu Lys Ile Glu Ile Glu Lys Ile

450 455 460

Leu Asn Glu Ser Ile Leu Ser Glu Lys Ile Leu Lys Arg Val Lys Gln

465 470 475 480

Tyr Thr Leu Glu His Ile Met Tyr Leu Gly Lys Leu Arg His Asn Asp

485 490 495

Ile Asp Met Thr Thr Val Asn Thr Asp Asp Phe Ser Arg Leu His Ala

500 505 510

Lys Glu Glu Leu Asp Leu Glu Leu Ile Thr Phe Phe Ala Ser Thr Asn

515 520 525

Met Glu Leu Asn Lys Ile Phe Ser Arg Glu Asn Ile Asn Asn Asp Glu

530 535 540

Asn Ile Asp Phe Phe Gly Gly Asp Arg Glu Lys Asn Tyr Val Leu Asp

545 550 555 560

Lys Lys Ile Leu Asn Ser Lys Ile Lys Ile Ile Arg Asp Leu Asp Phe

565 570 575

Ile Asp Asn Lys Asn Asn Ile Thr Asn Asn Phe Ile Arg Lys Phe Thr

580 585 590

Lys Ile Gly Thr Asn Glu Arg Asn Arg Ile Leu His Ala Ile Ser Lys

595 600 605

Glu Arg Asp Leu Gln Gly Thr Gln Asp Asp Tyr Asn Lys Val Ile Asn

610 615 620

Ile Ile Gln Asn Leu Lys Ile Ser Asp Glu Glu Val Ser Lys Ala Leu

625 630 635 640

Asn Leu Asp Val Val Phe Lys Asp Lys Lys Asn Ile Ile Thr Lys Ile

645 650 655

Asn Asp Ile Lys Ile Ser Glu Glu Asn Asn Asn Asp Ile Lys Tyr Leu

660 665 670

Pro Ser Phe Ser Lys Val Leu Pro Glu Ile Leu Asn Leu Tyr Arg Asn

675 680 685

Asn Pro Lys Asn Glu Pro Phe Asp Thr Ile Glu Thr Glu Lys Ile Val

690 695 700

Leu Asn Ala Leu Ile Tyr Val Asn Lys Glu Leu Tyr Lys Lys Leu Ile

705 710 715 720

Leu Glu Asp Asp Leu Glu Glu Asn Glu Ser Lys Asn Ile Phe Leu Gln

725 730 735

Glu Leu Lys Lys Thr Leu Gly Asn Ile Asp Glu Ile Asp Glu Asn Ile

740 745 750

Ile Glu Asn Tyr Tyr Lys Asn Ala Gln Ile Ser Ala Ser Lys Gly Asn

755 760 765

Asn Lys Ala Ile Lys Lys Tyr Gln Lys Lys Val Ile Glu Cys Tyr Ile

770 775 780

Gly Tyr Leu Arg Lys Asn Tyr Glu Glu Leu Phe Asp Phe Ser Asp Phe

785 790 795 800

Lys Met Asn Ile Gln Glu Ile Lys Lys Gln Ile Lys Asp Ile Asn Asp

805 810 815

Asn Lys Thr Tyr Glu Arg Ile Thr Val Lys Thr Ser Asp Lys Thr Ile

820 825 830

Val Ile Asn Asp Asp Phe Glu Tyr Ile Ile Ser Ile Phe Ala Leu Leu

835 840 845

Asn Ser Asn Ala Val Ile Asn Lys Ile Arg Asn Arg Phe Phe Ala Thr

850 855 860

Ser Val Trp Leu Asn Thr Ser Glu Tyr Gln Asn Ile Ile Asp Ile Leu

865 870 875 880

Asp Glu Ile Met Gln Leu Asn Thr Leu Arg Asn Glu Cys Ile Thr Glu

885 890 895

Asn Trp Asn Leu Asn Leu Glu Glu Phe Ile Gln Lys Met Lys Glu Ile

900 905 910

Glu Lys Asp Phe Asp Asp Phe Lys Ile Gln Thr Lys Lys Glu Ile Phe

915 920 925

Asn Asn Tyr Tyr Glu Asp Ile Lys Asn Asn Ile Leu Thr Glu Phe Lys

930 935 940

Asp Asp Ile Asn Gly Cys Asp Val Leu Glu Lys Lys Leu Glu Lys Ile

945 950 955 960

Val Ile Phe Asp Asp Glu Thr Lys Phe Glu Ile Asp Lys Lys Ser Asn

965 970 975

Ile Leu Gln Asp Glu Gln Arg Lys Leu Ser Asn Ile Asn Lys Lys Asp

980 985 990

Leu Lys Lys Lys Val Asp Gln Tyr Ile Lys Asp Lys Asp Gln Glu Ile

995 1000 1005

Lys Ser Lys Ile Leu Cys Arg Ile Ile Phe Asn Ser Asp Phe Leu Lys

1010 1015 1020

Lys Tyr Lys Lys Glu Ile Asp Asn Leu Ile Glu Asp Met Glu Ser Glu

1025 1030 1035 1040

Asn Glu Asn Lys Phe Gln Glu Ile Tyr Tyr Pro Lys Glu Arg Lys Asn

1045 1050 1055

Glu Leu Tyr Ile Tyr Lys Lys Asn Leu Phe Leu Asn Ile Gly Asn Pro

1060 1065 1070

Asn Phe Asp Lys Ile Tyr Gly Leu Ile Ser Asn Asp Ile Lys Met Ala

1075 1080 1085

Asp Ala Lys Phe Leu Phe Asn Ile Asp Gly Lys Asn Ile Arg Lys Asn

1090 1095 1100

Lys Ile Ser Glu Ile Asp Ala Ile Leu Lys Asn Leu Asn Asp Lys Leu

1105 1110 1115 1120

Asn Gly Tyr Ser Lys Glu Tyr Lys Glu Lys Tyr Ile Lys Lys Leu Lys

1125 1130 1135

Glu Asn Asp Asp Phe Phe Ala Lys Asn Ile Gln Asn Lys Asn Tyr Lys

1140 1145 1150

Ser Phe Glu Lys Asp Tyr Asn Arg Val Ser Glu Tyr Lys Lys Ile Arg

1155 1160 1165

Asp Leu Val Glu Phe Asn Tyr Leu Asn Lys Ile Glu Ser Tyr Leu Ile

1170 1175 1180

Asp Ile Asn Trp Lys Leu Ala Ile Gln Met Ala Arg Phe Glu Arg Asp

1185 1190 1195 1200

Met His Tyr Ile Val Asn Gly Leu Arg Glu Leu Gly Ile Ile Lys Leu

1205 1210 1215

Ser Gly Tyr Asn Thr Gly Ile Ser Arg Ala Tyr Pro Lys Arg Asn Gly

1220 1225 1230

Ser Asp Gly Phe Tyr Thr Thr Thr Ala Tyr Tyr Lys Phe Phe Asp Glu

1235 1240 1245

Glu Ser Tyr Lys Lys Phe Glu Lys Ile Cys Tyr Gly Phe Gly Ile Asp

1250 1255 1260

Leu Ser Glu Asn Ser Glu Ile Asn Lys Pro Glu Asn Glu Ser Ile Arg

1265 1270 1275 1280

Asn Tyr Ile Ser His Phe Tyr Ile Val Arg Asn Pro Phe Ala Asp Tyr

1285 1290 1295

Ser Ile Ala Glu Gln Ile Asp Arg Val Ser Asn Leu Leu Ser Tyr Ser

1300 1305 1310

Thr Arg Tyr Asn Asn Ser Thr Tyr Ala Ser Val Phe Glu Val Phe Lys

1315 1320 1325

Lys Asp Val Asn Leu Asp Tyr Asp Glu Leu Lys Lys Lys Phe Lys Leu

1330 1335 1340

Ile Gly Asn Asn Asp Ile Leu Glu Arg Leu Met Lys Pro Lys Lys Val

1345 1350 1355 1360

Ser Val Leu Glu Leu Glu Ser Tyr Asn Ser Asp Tyr Ile Lys Asn Leu

1365 1370 1375

Ile Ile Glu Leu Leu Thr Lys Ile Glu Asn Thr Asn Asp Thr Leu

1380 1385 1390

<210> 75

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 75

gctgtgatag gccccgattt gtggggtagt aacagc 36

<210> 76

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 76

gctggagcag cccccgattt gtggggtaat cacagc 36

<210> 77

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 77

ggctggagca gccctcgatt tgcagggtaa tcacagc 37

<210> 78

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 78

gctggagcag ccctcgattt gcagggtaat cacagc 36

<210> 79

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 79

gctggagcag cctcggattt gcggggtgat tacagc 36

<210> 80

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 80

gctggagtag cccccgattt gtggggtgat tacagc 36

<210> 81

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 81

gctgaagaag cctccgattt gtggggtgat tacagc 36

<210> 82

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 82

gctgtgatgg acctcgattt gtggggtagt aacagct 37

<210> 83

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 83

gctggagcag cccccgattt gtggggtaat cacagc 36

<210> 84

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 84

gttgtggttt gattaaagat tagagaacac gatatt 36

<210> 85

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 85

gttgtgactg ctcttatttt gaagggtaaa cacaac 36

<210> 86

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 86

ctttccattc cactatggtg caattttggg 30

<210> 87

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 87

atttccattc cactatggtg caattttggg 30

<210> 88

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 88

gagtccagat gacccccaga ttgaggggga ctaca 35

<210> 89

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 89

gctgtactca cccttcaaat aaagggcttt tacagc 36

<210> 90

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 90

gtcttagttc ccctttatgt ggggataatt tcaac 35

<210> 91

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 91

gttgaaatta tccccacata aaggggaact aagac 35

<210> 92

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 92

gttggagcag cccccgtttt gtggggtaat cacaac 36

<210> 93

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 93

gaactacacc cgtgcaaaat tgcaggggtc taaaac 36

<210> 94

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 94

tggtatagca ctctactgtt tgacagatgt ctaaaac 37

<210> 95

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 95

gttttagaca tctgtcaaac agtagagtgc tatacca 37

<210> 96

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 96

gttgtaactg ctctctgttt agagggtata aacaac 36

<210> 97

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 97

tgttgtaact gctcttagtt tgaagggtaa aaacaac 37

<210> 98

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 98

attaaaataa gcacgtttcg tgatggaaat 30

<210> 99

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 99

gagatagacc cttgttaact cgtaaggttc tgtgact 37

<210> 100

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 100

gtctggccgg gtgccgtcgg gtgcctggcc 30

<210> 101

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 101

gtttccgcgg cgaattcgcc gcggctccat tgaagc 36

<210> 102

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 102

gttacaacag ccccatagat tcgtggggtt ctgaaac 37

<210> 103

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 103

gttgtaactg cccttatttt gaagggtaaa aacaac 36

<210> 104

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 104

gctggagcag ccctcgattt gctgggtaat cacagc 36

<210> 105

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 105

gctgtgatta cccagcaaat cgagggctgc tccagc 36

<210> 106

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 106

tcctcgaagt tcatcacgcg ctcccacttg 30

<210> 107

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 107

caagtgggag cgcgtgatga acttcgagga 30

<210> 108

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 108

tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 109

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 109

cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60

cctacttgaa gctgtccttc ccc 83

<210> 110

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 110

tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60

tgcttcacgt aggccttgga gccgtacatg 90

<210> 111

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 111

cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60

ccatgtacgg ctccaaggcc tac 83

<210> 112

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 112

tcttgtggaa aggacgaaac accggctgtg ataggccccg atttgtgggg tagtaacagc 60

tcctcgaagt tcatcacgcg ctcccacttg 90

<210> 113

<211> 81

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 113

cctaatggat cctagtacaa aaaaagctgt tactacccca caaatcgggg cctatcacag 60

ccaagtggga gcgcgtgatg a 81

<210> 114

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 114

cagcgaagcg catgaactcc ttgatgatgg ccat 34

<210> 115

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 115

cagcatggcc atcatcaagg agttcatgcg cttc 34

<210> 116

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 116

cagcagccgt acatgaactg aggggacagg atgt 34

<210> 117

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 117

cagcacatcc tgtcccctca gttcatgtac ggct 34

<210> 118

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 118

cagcttcatc acgcgctccc acttgaagcc ctcg 34

<210> 119

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 119

cagccgaggg cttcaagtgg gagcgcgtga tgaa 34

<210> 120

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 120

cagcggtgcc gcgcagcttc accttgtaga tgaa 34

<210> 121

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 121

cagcttcatc tacaaggtga agctgcgcgg cacc 34

<210> 122

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 122

tcttgtggaa aggacgaaac accggctgga gcagcccccg atttgtgggg taatcacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 123

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 123

cctaatggat cctagtacaa aaaaagctgt gattacccca caaatcgggg gctgctccag 60

cctacttgaa gctgtccttc ccc 83

<210> 124

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 124

cagcggtgcc gcgcagcttc accttgtaga tgaa 34

<210> 125

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 125

cagcttcatc tacaaggtga agctgcgcgg cacc 34

<210> 126

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 126

cagcgaagcg catgaactcc ttgatgatgg ccat 34

<210> 127

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 127

cagcatggcc atcatcaagg agttcatgcg cttc 34

<210> 128

<211> 91

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 128

tcttgtggaa aggacgaaac accgggctgg agcagccctc gatttgcagg gtaatcacag 60

caagccctcg gggaaggaca gcttcaagta g 91

<210> 129

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 129

cctaatggat cctagtacaa aaaaagctgt gattaccctg caaatcgagg gctgctccag 60

ccctacttga agctgtcctt cccc 84

<210> 130

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 130

tcttgtggaa aggacgaaac accggctgga gcagccctcg atttgcaggg taatcacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 131

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 131

cctaatggat cctagtacaa aaaaagctgt gattaccctg caaatcgagg gctgctccag 60

cctacttgaa gctgtccttc ccc 83

<210> 132

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 132

tcttgtggaa aggacgaaac accggctgga gcagcctcgg atttgcgggg tgattacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 133

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 133

cctaatggat cctagtacaa aaaaagctgt aatcaccccg caaatccgag gctgctccag 60

cctacttgaa gctgtccttc ccc 83

<210> 134

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 134

cagcgaagcg catgaactcc ttgatgatgg ccat 34

<210> 135

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 135

cagcatggcc atcatcaagg agttcatgcg cttc 34

<210> 136

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 136

cagcagccgt acatgaactg aggggacagg atgt 34

<210> 137

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 137

cagcacatcc tgtcccctca gttcatgtac ggct 34

<210> 138

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 138

cagcttcatc acgcgctccc acttgaagcc ctcg 34

<210> 139

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 139

cagccgaggg cttcaagtgg gagcgcgtga tgaa 34

<210> 140

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 140

cagcggtgcc gcgcagcttc accttgtaga tgaa 34

<210> 141

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 141

cagcttcatc tacaaggtga agctgcgcgg cacc 34

<210> 142

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 142

cagcttgtag gtggtcttga cctcagcgtc gta 33

<210> 143

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 143

cagctacgac gctgaggtca agaccaccta caa 33

<210> 144

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 144

tcttgtggaa aggacgaaac accggctgga gtagcccccg atttgtgggg tgattacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 145

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 145

cctaatggat cctagtacaa aaaaagctgt aatcacccca caaatcgggg gctactccag 60

cctacttgaa gctgtccttc ccc 83

<210> 146

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 146

tcttgtggaa aggacgaaac accggctgaa gaagcctccg atttgtgggg tgattacagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 147

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 147

cctaatggat cctagtacaa aaaaagctgt aatcacccca caaatcggag gcttcttcag 60

cctacttgaa gctgtccttc ccc 83

<210> 148

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 148

cagcgaagcg catgaactcc ttgatgatgg ccat 34

<210> 149

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 149

cagcatggcc atcatcaagg agttcatgcg cttc 34

<210> 150

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 150

cagcagccgt acatgaactg aggggacagg atgt 34

<210> 151

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 151

cagcacatcc tgtcccctca gttcatgtac ggct 34

<210> 152

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 152

cagcttcatc acgcgctccc acttgaagcc ctcg 34

<210> 153

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 153

cagccgaggg cttcaagtgg gagcgcgtga tgaa 34

<210> 154

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 154

cagcggtgcc gcgcagcttc accttgtaga tgaa 34

<210> 155

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 155

cagcttcatc tacaaggtga agctgcgcgg cacc 34

<210> 156

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 156

cagcttgtag gtggtcttga cctcagcgtc gta 33

<210> 157

<211> 33

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 157

cagctacgac gctgaggtca agaccaccta caa 33

<210> 158

<211> 91

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 158

tcttgtggaa aggacgaaac accggctgtg atggacctcg atttgtgggg tagtaacagc 60

taagccctcg gggaaggaca gcttcaagta g 91

<210> 159

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 159

cctaatggat cctagtacaa aaaaaagctg ttactacccc acaaatcgag gtccatcaca 60

gcctacttga agctgtcctt cccc 84

<210> 160

<211> 91

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 160

tcttgtggaa aggacgaaac accggctgtg atggacctcg atttgtgggg tagtaacagc 60

ttgcttcacg taggccttgg agccgtacat g 91

<210> 161

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 161

cctaatggat cctagtacaa aaaaaagctg ttactacccc acaaatcgag gtccatcaca 60

gccatgtacg gctccaaggc ctac 84

<210> 162

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 162

tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60

atcctcgaag ttcatcacgc gctcccactt 90

<210> 163

<211> 82

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 163

cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60

accaagtggg agcgcgtgat ga 82

<210> 164

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 164

tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60

atgcttcacg taggccttgg agccgtacat 90

<210> 165

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 165

cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60

accatgtacg gctccaaggc ctac 84

<210> 166

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 166

tcttgtggaa aggacgaaac accggtttta gacatctgtc aaacagtaga gtgctatacc 60

aaagccctcg gggaaggaca gcttcaagta 90

<210> 167

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 167

cctaatggat cctagtacaa aaaaatggta tagcactcta ctgtttgaca gatgtctaaa 60

acctacttga agctgtcctt cccc 84

<210> 168

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 168

tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60

tcctcgaagt tcatcacgcg ctcccacttg 90

<210> 169

<211> 81

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 169

cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60

ccaagtggga gcgcgtgatg a 81

<210> 170

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 170

tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60

tgcttcacgt aggccttgga gccgtacatg 90

<210> 171

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 171

cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60

ccatgtacgg ctccaaggcc tac 83

<210> 172

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 172

tcttgtggaa aggacgaaac accggttgta actgctctct gtttagaggg tataaacaac 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 173

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 173

cctaatggat cctagtacaa aaaaagttgt ttataccctc taaacagaga gcagttacaa 60

cctacttgaa gctgtccttc ccc 83

<210> 174

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 174

tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaattcctcg 60

aagttcatca cgcgctccca cttg 84

<210> 175

<211> 75

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 175

cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatcaagt 60

gggagcgcgt gatga 75

<210> 176

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 176

tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaattgcttc 60

acgtaggcct tggagccgta catg 84

<210> 177

<211> 77

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 177

cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatcatgt 60

acggctccaa ggcctac 77

<210> 178

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 178

tcttgtggaa aggacgaaac accgattaaa ataagcacgt ttcgtgatgg aaataagccc 60

tcggggaagg acagcttcaa gtag 84

<210> 179

<211> 77

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 179

cctaatggat cctagtacaa aaaaaatttc catcacgaaa cgtgcttatt ttaatctact 60

tgaagctgtc cttcccc 77

<210> 180

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 180

tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60

ctcctcgaag ttcatcacgc gctcccactt 90

<210> 181

<211> 82

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 181

cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60

cacaagtggg agcgcgtgat ga 82

<210> 182

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 182

tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60

ctgcttcacg taggccttgg agccgtacat 90

<210> 183

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 183

cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60

cacatgtacg gctccaaggc ctac 84

<210> 184

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 184

tcttgtggaa aggacgaaac accgtgttgt aactgctctt agtttgaagg gtaaaaacaa 60

caagccctcg gggaaggaca gcttcaagta 90

<210> 185

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 185

cctaatggat cctagtacaa aaaaagttgt ttttaccctt caaactaaga gcagttacaa 60

cactacttga agctgtcctt cccc 84

<210> 186

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 186

tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60

ttcctcgaag ttcatcacgc gctcccactt 90

<210> 187

<211> 82

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 187

cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60

tccaagtggg agcgcgtgat ga 82

<210> 188

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 188

tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60

ttgcttcacg taggccttgg agccgtacat 90

<210> 189

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 189

cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60

tccatgtacg gctccaaggc ctac 84

<210> 190

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 190

tcttgtggaa aggacgaaac accggagata gacccttgtt aactcgtaag gttctgtgac 60

taagccctcg gggaaggaca gcttcaagta 90

<210> 191

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 191

cctaatggat cctagtacaa aaaaaagtca cagaacctta cgagttaaca agggtctatc 60

tcctacttga agctgtcctt cccc 84

<210> 192

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 192

tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggcctcctcg 60

aagttcatca cgcgctccca cttg 84

<210> 193

<211> 75

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 193

cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagaccaagt 60

gggagcgcgt gatga 75

<210> 194

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 194

tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggcctgcttc 60

acgtaggcct tggagccgta catg 84

<210> 195

<211> 77

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 195

cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagaccatgt 60

acggctccaa ggcctac 77

<210> 196

<211> 84

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 196

tcttgtggaa aggacgaaac accggtctgg ccgggtgccg tcgggtgcct ggccaagccc 60

tcggggaagg acagcttcaa gtag 84

<210> 197

<211> 77

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 197

cctaatggat cctagtacaa aaaaaggcca ggcacccgac ggcacccggc cagacctact 60

tgaagctgtc cttcccc 77

<210> 198

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 198

tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60

tcctcgaagt tcatcacgcg ctcccacttg 90

<210> 199

<211> 81

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 199

cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60

ccaagtggga gcgcgtgatg a 81

<210> 200

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 200

tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60

tgcttcacgt aggccttgga gccgtacatg 90

<210> 201

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 201

cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60

ccatgtacgg ctccaaggcc tac 83

<210> 202

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 202

tcttgtggaa aggacgaaac accggtttcc gcggcgaatt cgccgcggct ccattgaagc 60

aagccctcgg ggaaggacag cttcaagtag 90

<210> 203

<211> 83

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 203

cctaatggat cctagtacaa aaaaagcttc aatggagccg cggcgaattc gccgcggaaa 60

cctacttgaa gctgtccttc ccc 83

<210> 204

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 204

cagcactctc tctgggttga ggttgctgac cagc 34

<210> 205

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 205

cagcgctggt cagcaacctc aacccagaga gagt 34

<210> 206

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 206

cagcaggctt tggggtgtga ctctctctgg gttg 34

<210> 207

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 207

cagccaaccc agagagagtc acaccccaaa gcct 34

<210> 208

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 208

cagccagctg cacgttctgg tgcttcgag 29

<210> 209

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 209

cagcctcgaa gcaccagaac gtgcagctg 29

<210> 210

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 210

cagccactga tcacatttaa atggcttctc acctg 35

<210> 211

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 211

cagccaggtg agaagccatt taaatgtgat cagtg 35

<210> 212

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 212

cagcaggcca cataactgca ctgatcacat tt 32

<210> 213

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 213

cagcaaatgt gatcagtgca gttatgtggc ct 32

<210> 214

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 214

cagcgttact tcatgttgat tagaggccac at 32

<210> 215

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 215

cagcatgtgg cctctaatca acatgaagta ac 32

<210> 216

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 216

acagccaaat gctggtaaca ctgtggtcca caagg 35

<210> 217

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 217

cagcccttgt ggaccacagt gttaccagca tttgg 35

<210> 218

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 218

acagcgagag cagcagcaaa ctcctttgct ccctc 35

<210> 219

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 219

cagcgaggga gcaaaggagt ttgctgctgc tctcg 35

<210> 220

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 220

acagctttgc tccctccaaa tgctggtaac actgt 35

<210> 221

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 221

cagcacagtg ttaccagcat ttggagggag caaag 35

<210> 222

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 222

acagcctgta ctggtggatg tcctcaaaag acttg 35

<210> 223

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 223

cagccaagtc ttttgaggac atccaccagt acagg 35

<210> 224

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 224

acagctccga gtccttcacc cgtttgatct gctcc 35

<210> 225

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 225

cagcggagca gatcaaacgg gtgaaggact cggag 35

<210> 226

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 226

acagctgatc tgctccctgt actggtggat gtcct 35

<210> 227

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 227

cagcaggaca tccaccagta cagggagcag atcag 35

<210> 228

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 228

acagcgtttc tggcagttct cctctcctgc acccc 35

<210> 229

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 229

cagcggggtg caggagagga gaactgccag aaacg 35

<210> 230

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 230

acagcagcac tgctgggcac agatgatttt ggtca 35

<210> 231

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 231

cagctgacca aaatcatctg tgcccagcag tgctg 35

<210> 232

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 232

acagcgattt tggtcagttt ctggcagttc tcctc 35

<210> 233

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 233

cagcgaggag aactgccaga aactgaccaa aatcg 35

<210> 234

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 234

acagcctata atggtgaata tcttcaaatg attta 35

<210> 235

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 235

cagctaaatc atttgaagat attcaccatt atagg 35

<210> 236

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 236

acagctcaga gtccttaact cttttaattt gttct 35

<210> 237

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 237

cagcagaaca aattaaaaga gttaaggact ctgag 35

<210> 238

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 238

acagctaatt tgttctctat aatggtgaat atctt 35

<210> 239

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 239

cagcaagata ttcaccatta tagagaacaa attag 35

<210> 240

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 240

acagccttgg cctggacggc gtaagaagcc aggag 35

<210> 241

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 241

cagcctcctg gcttcttacg ccgtccaggc caagg 35

<210> 242

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 242

acagccttgt gaacactggg gtcgtagtca ccata 35

<210> 243

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 243

cagctatggt gactacgacc ccagtgttca caagg 35

<210> 244

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 244

acagctagtc accatacttg gcctggacgg cgtaa 35

<210> 245

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 245

cagcttacgc cgtccaggcc aagtatggtg actag 35

<210> 246

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 246

acagcctcat agttgtccat aagtgttttg gaagg 35

<210> 247

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 247

cagcccttcc aaaacactta tggacaacta tgagg 35

<210> 248

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 248

acagccacca gctctctgac tgtaccccca gagac 35

<210> 249

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 249

cagcgtctct gggggtacag tcagagagct ggtgg 35

<210> 250

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 250

acagcccccc agagacctca tagttgtcca taagt 35

<210> 251

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 251

cagcacttat ggacaactat gaggtctctg ggggg 35

<210> 252

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 252

acagcctgtc tggtcttggc tgaggtttca atgaa 35

<210> 253

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 253

cagcttcatt gaaacctcag ccaagaccag acagg 35

<210> 254

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 254

acagctacca gtgtgtaaaa agcatcttca acacc 35

<210> 255

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 255

cagcggtgtt gaagatgctt tttacacact ggtag 35

<210> 256

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 256

acagctcttc aacaccctgt ctggtcttgg ctgag 35

<210> 257

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 257

cagcctcagc caagaccaga cagggtgttg aagag 35

<210> 258

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 258

acagccatta tgagacatcc ccactgcaag gcatt 35

<210> 259

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 259

cagcaatgcc ttgcagtggg gatgtctcat aatgg 35

<210> 260

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 260

acagccggcc tgtggcatcc gcccaaacct gatgg 35

<210> 261

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 261

cagcccatca ggtttgggcg gatgccacag gccgg 35

<210> 262

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 262

acagcaaacc tgatggcatt atgagacatc cccac 35

<210> 263

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 263

cagcgtgggg atgtctcata atgccatcag gtttg 35

<210> 264

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 264

acagcctcaa tcatcctgct gtccacaggc agggt 35

<210> 265

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 265

cagcaccctg cctgtggaca gcaggatgat tgagg 35

<210> 266

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 266

acagcggctg attcgctgtg acttcgaatt gcatc 35

<210> 267

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 267

cagcgatgca attcgaagtc acagcgaatc agccg 35

<210> 268

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 268

acagccgaat tgcatcctca atcatcctgc tgtcc 35

<210> 269

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 269

cagcggacag caggatgatt gaggatgcaa ttcgg 35

<210> 270

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 270

acagcctggt gaggattcca gtcgctgtca aaaat 35

<210> 271

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 271

cagcattttt gacagcgact ggaatcctca ccagg 35

<210> 272

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 272

acagcgcggt gggctcggtc ctgcgcttgc aggtc 35

<210> 273

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 273

cagcgacctg caagcgcagg accgagccca ccgcg 35

<210> 274

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 274

acagcgcttg caggtcctgg tgaggattcc agtcg 35

<210> 275

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 275

cagccgactg gaatcctcac caggacctgc aagcg 35

<210> 276

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 276

acagcatcac aattggctcg gcccccattc ccaca 35

<210> 277

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 277

cagctgtggg aatgggggcc gagccaattg tgatg 35

<210> 278

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 278

acagcgtgca gctcctcagt cacaatcagg gaagc 35

<210> 279

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 279

cagcgcttcc ctgattgtga ctgaggagct gcacg 35

<210> 280

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 280

acagcatcag ggaagcatca caattggctc ggccc 35

<210> 281

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 281

cagcgggccg agccaattgt gatgcttccc tgatg 35

<210> 282

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 282

ttattccttc catcatcccg acagcagtgg 30

<210> 283

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 283

ccactgctgt cgggatgatg gaaggaataa 30

<210> 284

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 284

aagccctcgg ggaaggacag cttcaagtag 30

<210> 285

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 285

ctacttgaag ctgtccttcc ccgagggctt 30

<210> 286

<211> 10

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 286

ctcccacttg 10

<210> 287

<211> 10

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 287

caagtgggag 10

<210> 288

<211> 15

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 288

acgcgctccc acttg 15

<210> 289

<211> 15

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 289

caagtgggag cgcgt 15

<210> 290

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 290

tcatcacgcg ctcccacttg 20

<210> 291

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 291

caagtgggag cgcgtgatga 20

<210> 292

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 292

gaagttcatc acgcgctccc acttg 25

<210> 293

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 293

caagtgggag cgcgtgatga acttc 25

<210> 294

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 294

tcctcgaagt tcatcacgcg ctcccacttg 30

<210> 295

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 295

caagtgggag cgcgtgatga acttcgagga 30

<210> 296

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 296

cgccgtcctc gaagttcatc acgcgctccc acttg 35

<210> 297

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 297

caagtgggag cgcgtgatga acttcgagga cggcg 35

<210> 298

<211> 40

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 298

cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg 40

<210> 299

<211> 40

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 299

caagtgggag cgcgtgatga acttcgagga cggcggcgtg 40

<210> 300

<211> 45

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 300

gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttg 45

<210> 301

<211> 45

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 301

caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgac 45

<210> 302

<211> 50

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 302

tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg 50

<210> 303

<211> 50

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 303

caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 50